Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0003384

Physical Location & Seq

Genomic Coordinates Reverse (-)

14113866 .. 14116281

Gene Structure 2416 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027702

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGCGCCATGGAAGGCCTCTATTCCAACCCAAACCCCACATCGGCGGCGCTGCCGCTGCTTCTGAACTTGGAGACATACTCCTGGTGGCCTCCATCACCAAGACCCTCTCCCAGTCCGGCACCCGCAACCTCCCCCACCCGCCTCCTCCTCCTCTCTCCGACCCTCTCCTCCTCCAAATCCTCCGCACCCAATCCCTCCACCCCTCCAAGAAACTCGACTTCTTCAAATGGTCCTCTCTCACCCATTCCATCAAACACTCCCCCGCCACTTTCTCCCACATCCTCCGCACCGCCTGCCGCGCCGGGTTCCTCTCCGAAATCCCGGGACTACTTAACATCATGAAACAGCATGCTGTAATAGTCGATTCCGGAACCTTCAAGTCTTTGCTGGACGCCTTCATCAGGGCAGGTAAGTTTGACATGGCAATCGAAATTTTAGACATCATGCAAGAACTGGGTGCCGAGCTGAGTACTGATATGTACAACTCGGTGCTGGTTGCTTTAGTTAGGAAGGGTCAGTTGGGTTTGGGTATGTCTATTTTGGTTAAGCTTCTAGAAGGTGGGTCTTCTCAAGTACCCAATTGCATTGCTTGTAATGAGTTGTTGGTGGGTCTTAGGAAAGGTGACATGAGGGTAGAGTTTAAGCAGGTGTTTGATAAGCTTAGGGGGAATAAGTGGTTTGAGATGGATACTTGGGGTTATAATATTTGTATTCATGCGTTTGGGTGTTGGGGTGATTTGGGTACTAGTTTGAGTCTGTTTAAAGAGATGAAAGAGTTGAACTCCGACTCGGTTTTTCCTGATTTGTCTACTTATAATAGCCTCATTCATGTGCTGTGTTTGGTTGGGAAGGTGGATGATGCAGTTACTGTATGGGAGGAGTTGAAGTGTTCTGGTCATGAGCCGGATGCCATTACATACCGGATTCTTATTCAGGGGTGTTGTAAGTGTTATCGAATAAAGGAAGCTACTAAGATTTTTAGTGAAATGCAGAACAATGGGTACAATCCGGATACGGTTGTTTATAATTCGTTGATGGATGGGTTGTTCAAGGCGAGGAAAGTTAATGAAGGGTGTCAGATGTTTGAGAGGATGGTTCAGTATGGGGTGAGAGCCTCGACGTGGACGTATAATATTCTGATTGATGGGTTGTTTAGGAATGCAAGGGCCGAGGCTGCTTACACTATATTTTGTGACTTGAAGAAGAAGGGTCAGTTTGTGGATGGTGTTACATATAGCATTGTTGTTCTGCAACTTTGTAGGGAGGGTCTGCTCGAGGAGGCTCTAGAATTGGTGGAAGAAATGGAAATGCGAGGCTTTACTGTTGATTTGGTTACTATATCAACACTCATGATTGGGCTGTATAAGCATAGTCGATGGGATTGGACAGACAAGCTCATGAAGCGTATTAGGGATGGTAATCTGCTGCCTAGTGTTCTTAGATGGAAAGTAGATATGGAAGCTACAATGAAAGCTCCACAGAGCAAGAAAAAGGACCTTACACCACTGTTCCCAAGTAAAGGTGATTTCAGCGACGTTTTGAGTCTAATAAGTTCTGCTGCCTCGAAAATGGATGGAGTCATTGAATCAGATGATGCTGGGGTTAAAGATGATAATAATTCGTCTACAGATATTGATCAGTGGTCATCATCTCCACATATGGATCACTTGGCCAATCAATTGAAGTCCACTGACCACGCCTCTCAGCTGTTTTCACTATCTAGAGGGCAACGAGTTCAAGCCAAAGGAGAAAGCACTTTTGATATTGATATGGTAAATACTTTCTTATCCCTATTTTTGGCCAAGGGGAAGCTGAGCATAGCTTGCAAATTGTTTGAGATTTTCAGTGATGCGGGTGCAAACCCTATGAGTTACACTTATAATTCCATCTTGAGTTCGTTTGTCAAGAAGGGCTACTTCAATGAGGCATGGGGTGTGCTCAGTGAAATGGGAGAAAAAGTTTGCCCCACAGATATAGCAACATACAATATGATAATTCAAGGCTTGGGGAAGATGGGAAGGGCCGATCTAGCCAGTAGTGTTCTGGATAAGCTAATGAAGCAGGGTGGTTATCTTGATGTTGTCATGTACAACACCTTGATTAATGCGCTGGGGAAGGCTAACCGGATTGATGAAGTAAACAAGCTATTTAAGCAGATGAAGAGTAGTGGAATAAATCCTGATGTTGTCACTTTCAATACGCTTATTGAAGTTCATAGTAAAGCAGGTCGACTGAAAGATGCATATAAATTTCTGAAAATGATGCTGGATTCAGGCTGCACACCAAACCATGTCACTGACACCACTTTGGATTTTCTGGGGAAGGAGATTGAGAAATCGAGGTACCAAAAGGCATCATTTGTACGAAATAAGGATGACTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

795

Amino Acids

88.56

Weight (kDa)

7.51

Isoelectric Point (pI)

32.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	92 - 171	7e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	259 - 314	9.9e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	265 - 295	6e-09	PPR repeat
PPR_2	PF13041	267 - 316	4.1e-16	PPR repeat family
PPR_1	PF12854	299 - 331	7.2e-10	PPR repeat
PPR	PF01535	305 - 334	4.7e-06	PPR repeat
PPR_2	PF13041	309 - 340	8.2e-06	PPR repeat family
PPR_3	PF13812	325 - 382	1.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	333 - 366	1.3e-07	PPR repeat
PPR_2	PF13041	337 - 385	2.7e-14	PPR repeat family
PPR_2	PF13041	374 - 421	2.4e-10	PPR repeat family
PPR_1	PF12854	404 - 436	3e-07	PPR repeat
PPR	PF01535	410 - 440	8.1e-06	PPR repeat
PPR_2	PF13041	419 - 456	3.2e-06	PPR repeat family
PPR_2	PF13041	624 - 671	1.3e-11	PPR repeat family
PPR_3	PF13812	680 - 739	1.5e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	692 - 721	5.1e-07	PPR repeat
PPR_2	PF13041	693 - 736	1.8e-15	PPR repeat family
PPR	PF01535	695 - 725	3e-08	PPR repeat
PPR_3	PF13812	715 - 766	1.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	724 - 755	5.1e-10	PPR repeat
PPR_2	PF13041	727 - 770	3.4e-12	PPR repeat family
PPR	PF01535	730 - 760	3.1e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003384)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01570
fragaria_vesca	FvH4_7g18170
malus_domestica	MD01G1095900.v1.1 MD01G1096200.v1.1 MD01G1096300.v1.1 MD01G1097600.v1.1 MD01G1131700.v1.1
prunus_persica	Prupe.2G203600_v2.0.a1
pyrus_communis	pycom01g11790 pycom01g11820 pycom01g11830 pycom01g11890 pycom01g11900 pycom01g11920 pycom01g11930 pycom01g12020 pycom01g12110 pycom01g12190 pycom01g12290 pycom01g12360 pycom01g12370 pycom01g12440 pycom01g12450 pycom01g12460 pycom01g12490
rosa_chinensis	RchiOBHm_Chr1g0361811
rosa_laevigata	RLG00000027702
rosa_multiflora	Rmu_co8192272.1_g000001 Rmu_ssc0000124.1_g000020
rosa_roxburghii	Rroxscaffold_4G00294690 Rroxscaffold_5G00338610
rosa_rugosa	Rorug01G0291500
rosa_samantha	Rh1AG302000 Rh1BG265000 Rh1DG294900
rosa_wichuraiana	Rw1G026760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	702, 816, 1026, 1881
AarI	CACCTGC	1 cut(s)	637
Acc36I	ACCTGC	3 cut(s)	398, 637, 2216
Acc65I	GGTACC	1 cut(s)	2343
AccB1I	GGYRCC	3 cut(s)	119, 458, 2343
AccI	GTMKAC	3 cut(s)	809, 1625, 2230
AccII	CGCG	1 cut(s)	300
AccIII	TCCGGA	2 cut(s)	368, 1009
AclWI	GGATC	1 cut(s)	1671
AcoI	YGGCCR	2 cut(s)	1671, 1800
AcsI	RAATTY	2 cut(s)	432, 2249
AcyI	GRCGYC	1 cut(s)	393
AfaI	GTAC	8 cut(s)	472, 482, 576, 745, 1004, 2090, 2345, 2364
AfiI	CCNNNNNNNGG	5 cut(s)	41, 402, 1122, 1520, 1798
AhlI	ACTAGT	1 cut(s)	746
AjiI	CACGTC	1 cut(s)	1122
AjnI	CCWGG	1 cut(s)	81
AjuI	GAANNNNNNNTTGG	2 cut(s)	503, 535
AloI	GAACNNNNNNTCC	2 cut(s)	1031, 1063
Alw21I	GWGCWC	1 cut(s)	1941
Alw26I	GTCTC	1 cut(s)	66
AlwI	GGATC	1 cut(s)	1671
AlwNI	CAGNNNCTG	1 cut(s)	869
Ama87I	CYCGRG	2 cut(s)	322, 1274
Aor13HI	TCCGGA	2 cut(s)	368, 1009
AoxI	GGCC	6 cut(s)	14, 87, 1167, 1671, 1800, 2022
ApeKI	GCWGC	6 cut(s)	50, 56, 1175, 1426, 1559, 2277
ApoI	RAATTY	2 cut(s)	432, 2249
AseI	ATTAAT	1 cut(s)	2103
Asp718I	GGTACC	1 cut(s)	2343
AspLEI	GCGC	4 cut(s)	6, 50, 302, 2110
AspS9I	GGNCC	4 cut(s)	231, 1167, 1495, 2022
AsuC2I	CCSGG	3 cut(s)	304, 323, 324
AsuHPI	GGTGA	6 cut(s)	88, 232, 635, 746, 1120, 1535
AvaI	CYCGRG	2 cut(s)	322, 1274
AvaII	GGWCC	2 cut(s)	231, 1495
BaeI	ACNNNNGTAYC	2 cut(s)	1005, 1038
BalI	TGGCCA	2 cut(s)	1673, 1802
BanI	GGYRCC	3 cut(s)	119, 458, 2343
BbsI	GAAGAC	1 cut(s)	558
Bbv12I	GWGCWC	1 cut(s)	1941
BbvI	GCAGC	6 cut(s)	37, 43, 1162, 1413, 1546, 2264
BciT130I	CCWGG	1 cut(s)	83
BciVI	GTATCC	2 cut(s)	682, 1006
BclI	TGATCA	1 cut(s)	1636
BcnI	CCSGG	3 cut(s)	304, 323, 324
BcoDI	GTCTC	1 cut(s)	66
BcuI	ACTAGT	1 cut(s)	746
BfaI	CTAG	6 cut(s)	554, 747, 1286, 1431, 1722, 2030
BfmI	CTRYAG	1 cut(s)	1626
BfoI	RGCGCY	1 cut(s)	51
BfuAI	ACCTGC	3 cut(s)	398, 637, 2216
BfuI	GTATCC	2 cut(s)	682, 1006
BisI	GCNGC	9 cut(s)	46, 51, 54, 57, 298, 1176, 1427, 1560, 2278
BlpI	GCTNAGC	1 cut(s)	1814
BlsI	GCNGC	9 cut(s)	47, 52, 55, 58, 299, 1177, 1428, 1561, 2279
BmcAI	AGTACT	1 cut(s)	472
Bme1390I	CCNGG	4 cut(s)	83, 304, 323, 324
Bme18I	GGWCC	2 cut(s)	231, 1495
BmeT110I	CYCGRG	2 cut(s)	322, 1274
BmgBI	CACGTC	1 cut(s)	1122
BmgT120I	GGNCC	4 cut(s)	231, 1167, 1495, 2022
BmiI	GGNNCC	5 cut(s)	121, 308, 373, 460, 2345
BmrFI	CCNGG	4 cut(s)	83, 304, 323, 324
BmrI	ACTGGG	2 cut(s)	106, 464
BmsI	GCATC	7 cut(s)	850, 898, 1585, 1840, 2230, 2253, 2363
BmuI	ACTGGG	2 cut(s)	106, 464
BpiI	GAAGAC	1 cut(s)	558
BplI	GAGNNNNNCTC	4 cut(s)	63, 95, 767, 799
Bpu10I	CCTNAGC	1 cut(s)	662
Bpu1102I	GCTNAGC	1 cut(s)	1814
BpuEI	CTTGAG	2 cut(s)	555, 1912
BpuMI	CCSGG	3 cut(s)	304, 323, 324
BsaBI	GATNNNNATC	2 cut(s)	1419, 1635
BsaHI	GRCGYC	1 cut(s)	393
BsaJI	CCNNGG	4 cut(s)	7, 322, 1170, 1803
BsaWI	WCCGGW	4 cut(s)	368, 921, 1009, 2124
BsaXI	ACNNNNNCTCC	4 cut(s)	872, 902, 1944, 1974
Bsc4I	CCNNNNNNNGG	5 cut(s)	41, 402, 1122, 1520, 1798
Bse1I	ACTGG	3 cut(s)	112, 459, 2034
Bse3DI	GCAATG	1 cut(s)	585
Bse8I	GATNNNNATC	2 cut(s)	1419, 1635
BseAI	TCCGGA	2 cut(s)	368, 1009
BseBI	CCWGG	1 cut(s)	83
BseDI	CCNNGG	4 cut(s)	7, 322, 1170, 1803
BseGI	GGATG	9 cut(s)	279, 862, 913, 1045, 1098, 1228, 1420, 1579, 2380
BseJI	GATNNNNATC	2 cut(s)	1419, 1635
BseLI	CCNNNNNNNGG	5 cut(s)	41, 402, 1122, 1520, 1798
BseMI	GCAATG	1 cut(s)	585
BseMII	CTCAG	4 cut(s)	458, 1718, 1805, 1954
BseNI	ACTGG	3 cut(s)	112, 459, 2034
BseRI	GAGGAG	7 cut(s)	135, 138, 141, 158, 161, 893, 1292
BseXI	GCAGC	6 cut(s)	37, 43, 1162, 1413, 1546, 2264
BseYI	CCCAGC	2 cut(s)	1598, 2110
BsgI	GTGCAG	1 cut(s)	2263
Bsh1236I	CGCG	1 cut(s)	300
BshFI	GGCC	6 cut(s)	16, 89, 1169, 1673, 1802, 2024
BshNI	GGYRCC	3 cut(s)	119, 458, 2343
BsiHKAI	GWGCWC	1 cut(s)	1941
BsiHKCI	CYCGRG	2 cut(s)	322, 1274
BsiSI	CCGG	8 cut(s)	117, 303, 323, 369, 905, 922, 1010, 2125
BslFI	GGGAC	1 cut(s)	339
BslI	CCNNNNNNNGG	5 cut(s)	41, 402, 1122, 1520, 1798
BsmAI	GTCTC	1 cut(s)	66
BsmFI	GGGAC	1 cut(s)	339
BsmI	GAATGC	1 cut(s)	1165
BsnI	GGCC	6 cut(s)	16, 89, 1169, 1673, 1802, 2024
BsoBI	CYCGRG	2 cut(s)	322, 1274
Bsp1286I	GDGCHC	1 cut(s)	1941
Bsp13I	TCCGGA	2 cut(s)	368, 1009
Bsp1407I	TGTACA	2 cut(s)	480, 2088
Bsp143I	GATC	3 cut(s)	1636, 1663, 2026
Bsp1720I	GCTNAGC	1 cut(s)	1814
Bsp19I	CCATGG	1 cut(s)	7
BspANI	GGCC	6 cut(s)	16, 89, 1169, 1673, 1802, 2024
BspCNI	CTCAG	4 cut(s)	459, 1717, 1806, 1953
BspEI	TCCGGA	2 cut(s)	368, 1009
BspFNI	CGCG	1 cut(s)	300
BspHI	TCATGA	4 cut(s)	339, 898, 1350, 1398
BspLI	GGNNCC	5 cut(s)	121, 308, 373, 460, 2345
BspMI	ACCTGC	3 cut(s)	398, 637, 2216
BspPI	GGATC	1 cut(s)	1671
BspT107I	GGYRCC	3 cut(s)	119, 458, 2343
BsrDI	GCAATG	1 cut(s)	585
BsrGI	TGTACA	2 cut(s)	480, 2088
BsrI	ACTGG	3 cut(s)	112, 459, 2034
BssECI	CCNNGG	4 cut(s)	7, 322, 1170, 1803
BssMI	GATC	3 cut(s)	1636, 1663, 2026
BssNI	GRCGYC	1 cut(s)	393
BssT1I	CCWWGG	2 cut(s)	7, 1803
Bst2UI	CCWGG	1 cut(s)	83
Bst4CI	ACNGT	4 cut(s)	871, 1018, 1324, 1509
Bst6I	CTCTTC	1 cut(s)	2156
BstACI	GRCGYC	1 cut(s)	393
BstAPI	GCANNNNNTGC	2 cut(s)	294, 1824
BstAUI	TGTACA	2 cut(s)	480, 2088
BstC8I	GCNNGC	3 cut(s)	295, 351, 1825
BstDEI	CTNAG	8 cut(s)	467, 614, 662, 972, 1439, 1704, 1814, 1940
BstDSI	CCRYGG	1 cut(s)	7
BstENI	CCTNNNNNAGG	1 cut(s)	400
BstF5I	GGATG	9 cut(s)	279, 862, 913, 1045, 1098, 1228, 1420, 1579, 2380
BstFNI	CGCG	1 cut(s)	300
BstH2I	RGCGCY	1 cut(s)	51
BstHHI	GCGC	4 cut(s)	6, 50, 302, 2110
BstKTI	GATC	3 cut(s)	1639, 1666, 2029
BstMAI	GTCTC	1 cut(s)	66
BstMBI	GATC	3 cut(s)	1636, 1663, 2026
BstNI	CCWGG	1 cut(s)	83
BstNSI	RCATGY	1 cut(s)	353
BstSCI	CCNGG	4 cut(s)	81, 302, 321, 322
BstSFI	CTRYAG	1 cut(s)	1626
BstUI	CGCG	1 cut(s)	300
BstV1I	GCAGC	6 cut(s)	37, 43, 1162, 1413, 1546, 2264
BstV2I	GAAGAC	1 cut(s)	558
BsuI	GTATCC	2 cut(s)	682, 1006
BsuRI	GGCC	6 cut(s)	16, 89, 1169, 1673, 1802, 2024
BtgI	CCRYGG	1 cut(s)	7
BtrI	CACGTC	1 cut(s)	1122
BtsCI	GGATG	9 cut(s)	279, 862, 913, 1045, 1098, 1228, 1420, 1579, 2380
BtsIMutI	CAGTG	6 cut(s)	1505, 1646, 1689, 1852, 1948, 2295
BveI	ACCTGC	3 cut(s)	398, 637, 2216
Cac8I	GCNNGC	3 cut(s)	295, 351, 1825
CaiI	CAGNNNCTG	1 cut(s)	869
CciI	TCATGA	4 cut(s)	339, 898, 1350, 1398
CfoI	GCGC	4 cut(s)	6, 50, 302, 2110
Cfr13I	GGNCC	4 cut(s)	231, 1167, 1495, 2022
Cfr9I	CCCGGG	1 cut(s)	322
CseI	GACGC	1 cut(s)	401
Csp6I	GTAC	8 cut(s)	471, 481, 575, 744, 1003, 2089, 2344, 2363
CviQI	GTAC	8 cut(s)	471, 481, 575, 744, 1003, 2089, 2344, 2363
DdeI	CTNAG	8 cut(s)	467, 614, 662, 972, 1439, 1704, 1814, 1940
DpnI	GATC	3 cut(s)	1638, 1665, 2028
DpnII	GATC	3 cut(s)	1636, 1663, 2026
DraI	TTTAAA	1 cut(s)	763
EaeI	YGGCCR	2 cut(s)	1671, 1800
Eam1104I	CTCTTC	1 cut(s)	2156
EarI	CTCTTC	1 cut(s)	2156
Eco130I	CCWWGG	2 cut(s)	7, 1803
Eco147I	AGGCCT	1 cut(s)	16
Eco47I	GGWCC	2 cut(s)	231, 1495
Eco88I	CYCGRG	2 cut(s)	322, 1274
EcoNI	CCTNNNNNAGG	1 cut(s)	400
EcoO109I	RGGNCCY	1 cut(s)	1495
EcoRII	CCWGG	1 cut(s)	81
EcoT14I	CCWWGG	2 cut(s)	7, 1803
EcoT22I	ATGCAT	1 cut(s)	2245
ErhI	CCWWGG	2 cut(s)	7, 1803
FalI	AAGNNNNNCTT	2 cut(s)	1901, 1933
FaqI	GGGAC	1 cut(s)	339
FauI	CCCGC	4 cut(s)	131, 147, 271, 1846
FauNDI	CATATG	1 cut(s)	1659
FbaI	TGATCA	1 cut(s)	1636
FblI	GTMKAC	3 cut(s)	809, 1625, 2230
Fnu4HI	GCNGC	9 cut(s)	46, 51, 54, 57, 298, 1176, 1427, 1560, 2278
FokI	GGATG	8 cut(s)	266, 869, 920, 1052, 1105, 1235, 1427, 1586
Fsp4HI	GCNGC	9 cut(s)	46, 51, 54, 57, 298, 1176, 1427, 1560, 2278
FspBI	CTAG	6 cut(s)	554, 747, 1286, 1431, 1722, 2030
GlaI	GCGC	4 cut(s)	5, 49, 301, 2109
GluI	GCNGC	9 cut(s)	46, 51, 54, 57, 298, 1176, 1427, 1560, 2278
GsaI	CCCAGC	2 cut(s)	1602, 2114
HaeII	RGCGCY	1 cut(s)	51
HaeIII	GGCC	6 cut(s)	16, 89, 1169, 1673, 1802, 2024
HapII	CCGG	8 cut(s)	117, 303, 323, 369, 905, 922, 1010, 2125
HgaI	GACGC	1 cut(s)	401
HhaI	GCGC	4 cut(s)	6, 50, 302, 2110
Hin1I	GRCGYC	1 cut(s)	393
Hin6I	GCGC	4 cut(s)	4, 48, 300, 2108
HinP1I	GCGC	4 cut(s)	4, 48, 300, 2108
HincII	GTYRAC	1 cut(s)	2231
HindII	GTYRAC	1 cut(s)	2231
HindIII	AAGCTT	2 cut(s)	548, 659
HinfI	GANTC	9 cut(s)	365, 754, 788, 925, 1543, 1578, 1586, 2270, 2378
HpaII	CCGG	8 cut(s)	117, 303, 323, 369, 905, 922, 1010, 2125
HphI	GGTGA	6 cut(s)	88, 232, 635, 746, 1120, 1535
Hpy166II	GTNNAC	6 cut(s)	810, 1125, 1626, 1689, 2140, 2231
Hpy188I	TCNGA	8 cut(s)	64, 160, 316, 787, 1080, 1140, 1591, 2256
Hpy8I	GTNNAC	6 cut(s)	810, 1125, 1626, 1689, 2140, 2231
Hpy99I	CGWCG	2 cut(s)	1123, 1538
HpyCH4III	ACNGT	4 cut(s)	871, 1018, 1324, 1509
HpyCH4IV	ACGT	3 cut(s)	1121, 1127, 1536
HpyF3I	CTNAG	8 cut(s)	467, 614, 662, 972, 1439, 1704, 1814, 1940
HpySE526I	ACGT	3 cut(s)	1121, 1127, 1536
Hsp92I	GRCGYC	1 cut(s)	393
HspAI	GCGC	4 cut(s)	4, 48, 300, 2108
Kpn2I	TCCGGA	2 cut(s)	368, 1009
KpnI	GGTACC	1 cut(s)	2347
Ksp22I	TGATCA	1 cut(s)	1636
Kzo9I	GATC	3 cut(s)	1636, 1663, 2026
LmnI	GCTCC	1 cut(s)	1480
Lsp1109I	GCAGC	6 cut(s)	37, 43, 1162, 1413, 1546, 2264
LweI	GCATC	7 cut(s)	850, 898, 1585, 1840, 2230, 2253, 2363
MaeI	CTAG	6 cut(s)	554, 747, 1286, 1431, 1722, 2030
MaeII	ACGT	3 cut(s)	1121, 1127, 1536
MaeIII	GTNAC	8 cut(s)	623, 865, 1193, 1228, 1333, 1871, 2188, 2293
MalI	GATC	3 cut(s)	1638, 1665, 2028
MboI	GATC	3 cut(s)	1636, 1663, 2026
MboII	GAAGA	7 cut(s)	214, 558, 1213, 1216, 1310, 2023, 2173
MfeI	CAATTG	2 cut(s)	580, 1679
MhlI	GDGCHC	1 cut(s)	1941
MlsI	TGGCCA	2 cut(s)	1673, 1802
MluNI	TGGCCA	2 cut(s)	1673, 1802
MlyI	GAGTC	5 cut(s)	763, 782, 1552, 1587, 2372
MmeI	TCCRAC	3 cut(s)	49, 183, 810
Mox20I	TGGCCA	2 cut(s)	1673, 1802
Mph1103I	ATGCAT	1 cut(s)	2245
MroI	TCCGGA	2 cut(s)	368, 1009
MscI	TGGCCA	2 cut(s)	1673, 1802
MseI	TTAA	8 cut(s)	333, 546, 642, 762, 1065, 1605, 2103, 2151
MslI	CAYNNNNRTG	1 cut(s)	830
Msp20I	TGGCCA	2 cut(s)	1673, 1802
MspA1I	CMGCKG	2 cut(s)	56, 1708
MspI	CCGG	8 cut(s)	117, 303, 323, 369, 905, 922, 1010, 2125
MspR9I	CCNGG	4 cut(s)	83, 304, 323, 324
MunI	CAATTG	2 cut(s)	580, 1679
Mva1269I	GAATGC	1 cut(s)	1165
MvaI	CCWGG	1 cut(s)	83
MvnI	CGCG	1 cut(s)	300
NciI	CCSGG	3 cut(s)	304, 323, 324
NcoI	CCATGG	1 cut(s)	7
NdeI	CATATG	1 cut(s)	1659
NdeII	GATC	3 cut(s)	1636, 1663, 2026
NlaIV	GGNNCC	5 cut(s)	121, 308, 373, 460, 2345
NmeAIII	GCCGAG	2 cut(s)	487, 1195
NmuCI	GTSAC	4 cut(s)	623, 1193, 2188, 2293
NsiI	ATGCAT	1 cut(s)	2245
NspI	RCATGY	1 cut(s)	353
PaeI	GCATGC	1 cut(s)	353
PaeR7I	CTCGAG	1 cut(s)	1274
PagI	TCATGA	4 cut(s)	339, 898, 1350, 1398
PaqCI	CACCTGC	1 cut(s)	637
PceI	AGGCCT	1 cut(s)	16
PcsI	WCGNNNNNNNCGW	1 cut(s)	1124
PctI	GAATGC	1 cut(s)	1165
PfeI	GAWTC	4 cut(s)	365, 925, 1586, 2270
PkrI	GCNGC	9 cut(s)	47, 52, 55, 58, 299, 1177, 1428, 1561, 2279
PleI	GAGTC	5 cut(s)	762, 782, 1551, 1586, 2372
PpsI	GAGTC	5 cut(s)	762, 782, 1551, 1586, 2372
PpuMI	RGGWCCY	1 cut(s)	1495
PshBI	ATTAAT	1 cut(s)	2103
PsiI	TTATAA	4 cut(s)	702, 816, 1026, 1881
Psp5II	RGGWCCY	1 cut(s)	1495
Psp6I	CCWGG	1 cut(s)	81
PspFI	CCCAGC	2 cut(s)	1598, 2110
PspGI	CCWGG	1 cut(s)	81
PspN4I	GGNNCC	5 cut(s)	121, 308, 373, 460, 2345
PspPI	GGNCC	4 cut(s)	231, 1167, 1495, 2022
PspPPI	RGGWCCY	1 cut(s)	1495
PspXI	VCTCGAGB	1 cut(s)	1274
PsrI	GAACNNNNNNTAC	2 cut(s)	986, 1018
PstNI	CAGNNNCTG	1 cut(s)	869
PvuII	CAGCTG	1 cut(s)	1708
RsaI	GTAC	8 cut(s)	472, 482, 576, 745, 1004, 2090, 2345, 2364
RsaNI	GTAC	8 cut(s)	471, 481, 575, 744, 1003, 2089, 2344, 2363
RseI	CAYNNNNRTG	1 cut(s)	830
SalI	GTCGAC	1 cut(s)	2229
SaqAI	TTAA	8 cut(s)	333, 546, 642, 762, 1065, 1605, 2103, 2151
SatI	GCNGC	9 cut(s)	46, 51, 54, 57, 298, 1176, 1427, 1560, 2278
Sau3AI	GATC	3 cut(s)	1636, 1663, 2026
Sau96I	GGNCC	4 cut(s)	231, 1167, 1495, 2022
ScaI	AGTACT	1 cut(s)	472
SchI	GAGTC	5 cut(s)	763, 782, 1552, 1587, 2372
ScrFI	CCNGG	4 cut(s)	83, 304, 323, 324
SduI	GDGCHC	1 cut(s)	1941
SfaNI	GCATC	7 cut(s)	850, 898, 1585, 1840, 2230, 2253, 2363
SfcI	CTRYAG	1 cut(s)	1626
Sfr274I	CTCGAG	1 cut(s)	1274
SinI	GGWCC	2 cut(s)	231, 1495
SlaI	CTCGAG	1 cut(s)	1274
SmaI	CCCGGG	1 cut(s)	324
SmiMI	CAYNNNNRTG	1 cut(s)	830
SmlI	CTYRAG	3 cut(s)	570, 1274, 1891
SmoI	CTYRAG	3 cut(s)	570, 1274, 1891
SpeI	ACTAGT	1 cut(s)	746
SphI	GCATGC	1 cut(s)	353
SseBI	AGGCCT	1 cut(s)	16
SspI	AATATT	2 cut(s)	706, 1135
SspMI	CTAG	6 cut(s)	554, 747, 1286, 1431, 1722, 2030
StuI	AGGCCT	1 cut(s)	16
StyD4I	CCNGG	4 cut(s)	81, 302, 321, 322
StyI	CCWWGG	2 cut(s)	7, 1803
TaaI	ACNGT	4 cut(s)	871, 1018, 1324, 1509
TaiI	ACGT	3 cut(s)	1124, 1130, 1539
TatI	WGTACW	3 cut(s)	470, 480, 2088
TauI	GCSGC	3 cut(s)	48, 56, 300
TfiI	GAWTC	4 cut(s)	365, 925, 1586, 2270
Tru1I	TTAA	8 cut(s)	333, 546, 642, 762, 1065, 1605, 2103, 2151
Tru9I	TTAA	8 cut(s)	333, 546, 642, 762, 1065, 1605, 2103, 2151
TscAI	CASTG	6 cut(s)	1512, 1646, 1696, 1852, 1948, 2302
TseFI	GTSAC	4 cut(s)	623, 1193, 2188, 2293
TseI	GCWGC	6 cut(s)	50, 56, 1175, 1426, 1559, 2277
Tsp45I	GTSAC	4 cut(s)	623, 1193, 2188, 2293
TspMI	CCCGGG	1 cut(s)	322
TspRI	CASTG	6 cut(s)	1512, 1646, 1696, 1852, 1948, 2302
VpaK11BI	GGWCC	2 cut(s)	231, 1495
VspI	ATTAAT	1 cut(s)	2103
XagI	CCTNNNNNAGG	1 cut(s)	400
XapI	RAATTY	2 cut(s)	432, 2249
XbaI	TCTAGA	3 cut(s)	553, 1285, 1721
XceI	RCATGY	1 cut(s)	353
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2041
XhoI	CTCGAG	1 cut(s)	1274
XmaI	CCCGGG	1 cut(s)	322
XmiI	GTMKAC	3 cut(s)	809, 1625, 2230
XspI	CTAG	6 cut(s)	554, 747, 1286, 1431, 1722, 2030
ZrmI	AGTACT	1 cut(s)	472
Zsp2I	ATGCAT	1 cut(s)	2245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.