Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001661

Physical Location & Seq

Genomic Coordinates Reverse (-)

27919872 .. 27928159

Gene Structure 8288 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1179500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGTGAAAGCCATTAGAGTTCATGAACTTGGTGGTCCTCAGGTCTTGAAATGGGAAGAAGTGGAAATAGGAGAGCCGAAGGAGGGGGAGGTTCGTGTGAAGAAGAAGGCGATAGGGGTTAATTTCATCGATGTATACTTCCGCAAAGGACTTTATTATAAGGCTGATACAATGCCCTTCTCCCCAGGTATGGAAGCTTGTGGAGTTGTGACAGCCGTAGGACCTGGAGTAACAGACAGGCGAGTTGGAGATCTGGTGGCCTATGTTGGCACTCCGATGGGGTCATATGCGGAAGAGCAGATCCTTCCTGAAAACAAAGTGGTGCCGGTTCCCCCTTCAATTGATCCTATTGTTGCAGCATCGGTATTGCTTAAGGGTATGACAGCTCAGGTTCTACTACGTCAGTCTTTCAAGGTCAAACCAGGACACACAGTCCTTGTTCACGCAGCAGCTGGTGGAGTTGGATCCCTTTTATGCCAGTGGGCTAATGCGCTTGGTGCAACTGTCATTGGAACTGTATCAACTAAAGAGAAGACAGCTCAGGCCAAGGATGATGGGTGTCACCATGTCATAGTCTACACGAAAGAGGATTTTGTTGCTCGAGTGACAGAAATAACATCTGACAATGGGGTTGATGTTGTCTATGATTCTGTAGGGAAGGATACCTTTCAGGGATCATTGGCATGCTTGAAGACTCGTGGATACATGGTGAGTTTCGGACAAGCATCAGGTGCAATTGATCCCGTCCCACTATCAGCTCTTGCAGCAAACTCACTCTTCCTGACCAGACCGGCCCTGTTCCAGTACACTGCAGCTCGAGACAAGTTGTTAGAGGTTGCGGGAGAGGTATTTGCTAATGTTGCATCAGGCGTATTGCGAGTTCGAGTAAATCACAAATACCCATTGTCTCACGCAGCACAAGCGCATGCAGACCTCGAGAACAGGAAAACATCAGGCTCGGCTGTGCTTATACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003960 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009653 GO:0009664 GO:0009826 GO:0009827 GO:0009828 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016491 GO:0016651 GO:0016655 GO:0017091 GO:0030312 GO:0032502 GO:0032989 GO:0033554 GO:0034599 GO:0040007 GO:0042221 GO:0042545 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051716 GO:0055114 GO:0060560 GO:0070887 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

326

Amino Acids

34.6

Weight (kDa)

8.23

Isoelectric Point (pI)

27.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	28 - 111	2.3e-15	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	153 - 258	8.5e-25	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	186 - 323	5.4e-21	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61510 AT5G61510
fragaria_vesca	FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27140
malus_domestica	MD01G1178400.v1.1 MD01G1179500.v1.1 MD01G1180600.v1.1 MD07G1250800.v1.1 MD07G1251000.v1.1
prunus_persica	Prupe.2G133300_v2.0.a1 Prupe.2G133300_v2.0.a1 Prupe.2G274500_v2.0.a1 Prupe.2G274600_v2.0.a1
pyrus_communis	pycom01g18920 pycom01g19010 pycom01g19080 pycom07g22370
rosa_chinensis	RchiOBHm_Chr1g0363511 RchiOBHm_Chr1g0373931 RchiOBHm_Chr1g0373941
rosa_laevigata	RLG00000026793 RLG00000026794 RLG00000027560
rosa_multiflora	Rmu_sc0002022.1_g000001 Rmu_sc0002740.1_g000012 Rmu_sc0008820.1_g000007
rosa_roxburghii	Rroxscaffold_4G00283890 Rroxscaffold_4G00293100
rosa_rugosa	Rorug01G0308000 Rorug01G0379800 Rorug01G0379900
rosa_samantha	Rh1AG316700 Rh1AG390100 Rh1AG390200 Rh1BG280100 Rh1BG354400 Rh1BG354500 Rh1CG296200 Rh1CG367500 Rh1CG367600 Rh1DG311300 Rh1DG385100 Rh1DG385200
rosa_wichuraiana	Rw1G028070 Rw1G034500 Rw1G034510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	159
AccB1I	GGYRCC	1 cut(s)	322
AccI	GTMKAC	2 cut(s)	135, 576
AciI	CCGC	3 cut(s)	142, 290, 839
AclWI	GGATC	6 cut(s)	295, 338, 459, 472, 682, 734
AfaI	GTAC	1 cut(s)	806
AfiI	CCNNNNNNNGG	2 cut(s)	83, 190
AflII	CTTAAG	1 cut(s)	371
AgsI	TTSAA	4 cut(s)	49, 339, 412, 691
AhdI	GACNNNNNGTC	1 cut(s)	431
AjnI	CCWGG	3 cut(s)	184, 223, 421
AluBI	AGCT	6 cut(s)	197, 386, 452, 539, 758, 815
AluI	AGCT	6 cut(s)	197, 386, 452, 539, 758, 815
Alw26I	GTCTC	2 cut(s)	813, 912
AlwI	GGATC	6 cut(s)	295, 338, 459, 472, 682, 734
AlwNI	CAGNNNCTG	1 cut(s)	452
Ama87I	CYCGRG	3 cut(s)	600, 816, 935
AoxI	GGCC	3 cut(s)	258, 543, 792
ApeKI	GCWGC	6 cut(s)	356, 446, 449, 764, 812, 914
AspLEI	GCGC	2 cut(s)	493, 925
AspS9I	GGNCC	3 cut(s)	35, 221, 793
AsuHPI	GGTGA	3 cut(s)	16, 554, 721
AvaI	CYCGRG	3 cut(s)	600, 816, 935
AvaII	GGWCC	2 cut(s)	35, 221
AxyI	CCTNAGG	1 cut(s)	39
BamHI	GGATCC	1 cut(s)	464
BanI	GGYRCC	1 cut(s)	322
BauI	CACGAG	1 cut(s)	696
BbsI	GAAGAC	2 cut(s)	539, 698
BbvI	GCAGC	6 cut(s)	368, 458, 461, 776, 824, 926
BccI	CCATC	2 cut(s)	271, 548
BceAI	ACGGC	1 cut(s)	200
BciT130I	CCWGG	3 cut(s)	186, 225, 423
BciVI	GTATCC	2 cut(s)	655, 695
BcoDI	GTCTC	2 cut(s)	813, 912
BfmI	CTRYAG	2 cut(s)	651, 810
BfrI	CTTAAG	1 cut(s)	371
BfuI	GTATCC	2 cut(s)	655, 695
BglII	AGATCT	1 cut(s)	250
BisI	GCNGC	6 cut(s)	357, 447, 450, 765, 813, 915
BlsI	GCNGC	6 cut(s)	358, 448, 451, 766, 814, 916
Bme1390I	CCNGG	3 cut(s)	186, 225, 423
Bme18I	GGWCC	2 cut(s)	35, 221
BmeRI	GACNNNNNGTC	1 cut(s)	431
BmeT110I	CYCGRG	3 cut(s)	600, 816, 935
BmgT120I	GGNCC	3 cut(s)	35, 221, 793
BmiI	GGNNCC	3 cut(s)	324, 330, 466
BmrFI	CCNGG	3 cut(s)	186, 225, 423
BmsI	GCATC	3 cut(s)	368, 734, 872
BpiI	GAAGAC	2 cut(s)	539, 698
BpmI	CTGGAG	1 cut(s)	246
Bpu10I	CCTNAGC	2 cut(s)	387, 540
Bsa29I	ATCGAT	1 cut(s)	129
BsaJI	CCNNGG	2 cut(s)	184, 546
BsaXI	ACNNNNNCTCC	2 cut(s)	240, 270
Bsc4I	CCNNNNNNNGG	2 cut(s)	83, 190
Bse118I	RCCGGY	2 cut(s)	325, 790
Bse1I	ACTGG	2 cut(s)	478, 802
Bse21I	CCTNAGG	1 cut(s)	39
BseBI	CCWGG	3 cut(s)	186, 225, 423
BseCI	ATCGAT	1 cut(s)	129
BseDI	CCNNGG	2 cut(s)	184, 546
BseGI	GGATG	1 cut(s)	556
BseLI	CCNNNNNNNGG	2 cut(s)	83, 190
BseMII	CTCAG	3 cut(s)	53, 401, 554
BseNI	ACTGG	2 cut(s)	478, 802
BseXI	GCAGC	6 cut(s)	368, 458, 461, 776, 824, 926
BshFI	GGCC	3 cut(s)	260, 545, 794
BshNI	GGYRCC	1 cut(s)	322
BshVI	ATCGAT	1 cut(s)	129
BsiHKCI	CYCGRG	3 cut(s)	600, 816, 935
BsiSI	CCGG	2 cut(s)	326, 791
BslFI	GGGAC	1 cut(s)	731
BslI	CCNNNNNNNGG	2 cut(s)	83, 190
BsmAI	GTCTC	2 cut(s)	813, 912
BsmFI	GGGAC	1 cut(s)	731
BsnI	GGCC	3 cut(s)	260, 545, 794
BsoBI	CYCGRG	3 cut(s)	600, 816, 935
Bsp143I	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
BspACI	CCGC	3 cut(s)	142, 290, 839
BspANI	GGCC	3 cut(s)	260, 545, 794
BspCNI	CTCAG	3 cut(s)	52, 400, 553
BspDI	ATCGAT	1 cut(s)	129
BspHI	TCATGA	1 cut(s)	22
BspLI	GGNNCC	3 cut(s)	324, 330, 466
BspMAI	CTGCAG	1 cut(s)	814
BspPI	GGATC	6 cut(s)	295, 338, 459, 472, 682, 734
BspQI	GCTCTTC	1 cut(s)	288
BspT107I	GGYRCC	1 cut(s)	322
BspTI	CTTAAG	1 cut(s)	371
BsrFI	RCCGGY	2 cut(s)	325, 790
BsrI	ACTGG	2 cut(s)	478, 802
BssAI	RCCGGY	2 cut(s)	325, 790
BssECI	CCNNGG	2 cut(s)	184, 546
BssMI	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
BssNAI	GTATAC	1 cut(s)	136
BssSI	CACGAG	1 cut(s)	696
BssT1I	CCWWGG	1 cut(s)	546
Bst1107I	GTATAC	1 cut(s)	136
Bst2BI	CACGAG	1 cut(s)	696
Bst2UI	CCWGG	3 cut(s)	186, 225, 423
Bst4CI	ACNGT	3 cut(s)	433, 505, 517
Bst6I	CTCTTC	2 cut(s)	288, 782
BstAFI	CTTAAG	1 cut(s)	371
BstAPI	GCANNNNNTGC	1 cut(s)	731
BstC8I	GCNNGC	2 cut(s)	685, 927
BstDEI	CTNAG	3 cut(s)	39, 387, 540
BstF5I	GGATG	1 cut(s)	556
BstHHI	GCGC	2 cut(s)	493, 925
BstKTI	GATC	6 cut(s)	253, 303, 346, 467, 677, 742
BstMAI	GTCTC	2 cut(s)	813, 912
BstMBI	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
BstMWI	GCNNNNNNNGC	5 cut(s)	497, 731, 764, 860, 920
BstNI	CCWGG	3 cut(s)	186, 225, 423
BstNSI	RCATGY	2 cut(s)	687, 929
BstSCI	CCNGG	3 cut(s)	184, 223, 421
BstSFI	CTRYAG	2 cut(s)	651, 810
BstV1I	GCAGC	6 cut(s)	368, 458, 461, 776, 824, 926
BstV2I	GAAGAC	2 cut(s)	539, 698
BstX2I	RGATCY	3 cut(s)	250, 300, 464
BstYI	RGATCY	3 cut(s)	250, 300, 464
BstZ17I	GTATAC	1 cut(s)	136
Bsu15I	ATCGAT	1 cut(s)	129
Bsu36I	CCTNAGG	1 cut(s)	39
BsuI	GTATCC	2 cut(s)	655, 695
BsuRI	GGCC	3 cut(s)	260, 545, 794
BsuTUI	ATCGAT	1 cut(s)	129
BtsCI	GGATG	1 cut(s)	556
BtsI	GCAGTG	1 cut(s)	807
BtsIMutI	CAGTG	2 cut(s)	485, 807
Cac8I	GCNNGC	2 cut(s)	685, 927
CaiI	CAGNNNCTG	1 cut(s)	452
CciI	TCATGA	1 cut(s)	22
CfoI	GCGC	2 cut(s)	493, 925
Cfr10I	RCCGGY	2 cut(s)	325, 790
Cfr13I	GGNCC	3 cut(s)	35, 221, 793
ClaI	ATCGAT	1 cut(s)	129
Csp6I	GTAC	1 cut(s)	805
CviAII	CATG	5 cut(s)	23, 566, 684, 706, 926
CviQI	GTAC	1 cut(s)	805
DdeI	CTNAG	3 cut(s)	39, 387, 540
DpnI	GATC	6 cut(s)	252, 302, 345, 466, 676, 741
DpnII	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
DriI	GACNNNNNGTC	1 cut(s)	431
Eam1104I	CTCTTC	2 cut(s)	288, 782
Eam1105I	GACNNNNNGTC	1 cut(s)	431
EarI	CTCTTC	2 cut(s)	288, 782
Eco130I	CCWWGG	1 cut(s)	546
Eco47I	GGWCC	2 cut(s)	35, 221
Eco81I	CCTNAGG	1 cut(s)	39
Eco88I	CYCGRG	3 cut(s)	600, 816, 935
EcoO109I	RGGNCCY	1 cut(s)	221
EcoRII	CCWGG	3 cut(s)	184, 223, 421
EcoT14I	CCWWGG	1 cut(s)	546
ErhI	CCWWGG	1 cut(s)	546
FaeI	CATG	5 cut(s)	26, 569, 687, 709, 929
FalI	AAGNNNNNCTT	2 cut(s)	650, 682
FaqI	GGGAC	1 cut(s)	731
FatI	CATG	5 cut(s)	22, 565, 683, 705, 925
FauI	CCCGC	1 cut(s)	832
FauNDI	CATATG	1 cut(s)	286
FblI	GTMKAC	2 cut(s)	135, 576
Fnu4HI	GCNGC	6 cut(s)	357, 447, 450, 765, 813, 915
FokI	GGATG	1 cut(s)	563
Fsp4HI	GCNGC	6 cut(s)	357, 447, 450, 765, 813, 915
GlaI	GCGC	2 cut(s)	492, 924
GluI	GCNGC	6 cut(s)	357, 447, 450, 765, 813, 915
GsuI	CTGGAG	1 cut(s)	246
HaeIII	GGCC	3 cut(s)	260, 545, 794
HapII	CCGG	2 cut(s)	326, 791
HhaI	GCGC	2 cut(s)	493, 925
Hin1II	CATG	5 cut(s)	26, 569, 687, 709, 929
Hin6I	GCGC	2 cut(s)	491, 923
HinP1I	GCGC	2 cut(s)	491, 923
HindIII	AAGCTT	1 cut(s)	195
HinfI	GANTC	2 cut(s)	647, 694
HpaII	CCGG	2 cut(s)	326, 791
HphI	GGTGA	3 cut(s)	16, 554, 721
Hpy166II	GTNNAC	4 cut(s)	136, 442, 577, 807
Hpy188I	TCNGA	3 cut(s)	276, 622, 719
Hpy188III	TCNNGA	6 cut(s)	23, 46, 308, 781, 818, 937
Hpy8I	GTNNAC	4 cut(s)	136, 442, 577, 807
HpyAV	CCTTC	6 cut(s)	73, 100, 187, 314, 345, 652
HpyCH4III	ACNGT	3 cut(s)	433, 505, 517
HpyCH4IV	ACGT	1 cut(s)	400
HpyCH4V	TGCA	7 cut(s)	356, 500, 734, 764, 812, 863, 929
HpyF10VI	GCNNNNNNNGC	5 cut(s)	497, 731, 764, 860, 920
HpyF3I	CTNAG	3 cut(s)	39, 387, 540
HpySE526I	ACGT	1 cut(s)	400
Hsp92II	CATG	5 cut(s)	26, 569, 687, 709, 929
HspAI	GCGC	2 cut(s)	491, 923
Kzo9I	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
LguI	GCTCTTC	1 cut(s)	288
Lsp1109I	GCAGC	6 cut(s)	368, 458, 461, 776, 824, 926
LweI	GCATC	3 cut(s)	368, 734, 872
MaeII	ACGT	1 cut(s)	400
MaeIII	GTNAC	4 cut(s)	208, 229, 560, 604
MalI	GATC	6 cut(s)	252, 302, 345, 466, 676, 741
MboI	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
MboII	GAAGA	7 cut(s)	68, 112, 115, 305, 544, 703, 769
MfeI	CAATTG	2 cut(s)	339, 735
MflI	RGATCY	3 cut(s)	250, 300, 464
MluCI	AATT	3 cut(s)	121, 339, 735
MlyI	GAGTC	1 cut(s)	688
MmeI	TCCRAC	2 cut(s)	226, 442
MnlI	CCTC	7 cut(s)	48, 76, 82, 580, 826, 838, 944
MseI	TTAA	2 cut(s)	120, 372
MslI	CAYNNNNRTG	2 cut(s)	275, 682
MspA1I	CMGCKG	1 cut(s)	452
MspCI	CTTAAG	1 cut(s)	371
MspI	CCGG	2 cut(s)	326, 791
MspR9I	CCNGG	3 cut(s)	186, 225, 423
MunI	CAATTG	2 cut(s)	339, 735
MvaI	CCWGG	3 cut(s)	186, 225, 423
MwoI	GCNNNNNNNGC	5 cut(s)	497, 731, 764, 860, 920
NdeI	CATATG	1 cut(s)	286
NdeII	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
NlaIII	CATG	5 cut(s)	26, 569, 687, 709, 929
NlaIV	GGNNCC	3 cut(s)	324, 330, 466
NmeAIII	GCCGAG	1 cut(s)	938
NmuCI	GTSAC	3 cut(s)	208, 560, 604
NspI	RCATGY	2 cut(s)	687, 929
PaeI	GCATGC	2 cut(s)	687, 929
PaeR7I	CTCGAG	3 cut(s)	600, 816, 935
PagI	TCATGA	1 cut(s)	22
PciSI	GCTCTTC	1 cut(s)	288
PfeI	GAWTC	1 cut(s)	647
PkrI	GCNGC	6 cut(s)	358, 448, 451, 766, 814, 916
PleI	GAGTC	1 cut(s)	688
PpsI	GAGTC	1 cut(s)	688
PpuMI	RGGWCCY	1 cut(s)	221
PsiI	TTATAA	1 cut(s)	159
Psp5II	RGGWCCY	1 cut(s)	221
Psp6I	CCWGG	3 cut(s)	184, 223, 421
PspGI	CCWGG	3 cut(s)	184, 223, 421
PspN4I	GGNNCC	3 cut(s)	324, 330, 466
PspPI	GGNCC	3 cut(s)	35, 221, 793
PspPPI	RGGWCCY	1 cut(s)	221
PspXI	VCTCGAGB	1 cut(s)	600
PsrI	GAACNNNNNNTAC	2 cut(s)	864, 896
PstI	CTGCAG	1 cut(s)	814
PstNI	CAGNNNCTG	1 cut(s)	452
PsuI	RGATCY	3 cut(s)	250, 300, 464
PvuII	CAGCTG	1 cut(s)	452
RsaI	GTAC	1 cut(s)	806
RsaNI	GTAC	1 cut(s)	805
RseI	CAYNNNNRTG	2 cut(s)	275, 682
SapI	GCTCTTC	1 cut(s)	288
SaqAI	TTAA	2 cut(s)	120, 372
SatI	GCNGC	6 cut(s)	357, 447, 450, 765, 813, 915
Sau3AI	GATC	6 cut(s)	250, 300, 343, 464, 674, 739
Sau96I	GGNCC	3 cut(s)	35, 221, 793
SchI	GAGTC	1 cut(s)	688
ScrFI	CCNGG	3 cut(s)	186, 225, 423
SfaNI	GCATC	3 cut(s)	368, 734, 872
SfcI	CTRYAG	2 cut(s)	651, 810
Sfr274I	CTCGAG	3 cut(s)	600, 816, 935
SinI	GGWCC	2 cut(s)	35, 221
SlaI	CTCGAG	3 cut(s)	600, 816, 935
SmiMI	CAYNNNNRTG	2 cut(s)	275, 682
SmlI	CTYRAG	4 cut(s)	371, 600, 816, 935
SmoI	CTYRAG	4 cut(s)	371, 600, 816, 935
SphI	GCATGC	2 cut(s)	687, 929
Sse9I	AATT	3 cut(s)	121, 339, 735
SsiI	CCGC	3 cut(s)	142, 290, 839
StyD4I	CCNGG	3 cut(s)	184, 223, 421
StyI	CCWWGG	1 cut(s)	546
TaaI	ACNGT	3 cut(s)	433, 505, 517
TaiI	ACGT	1 cut(s)	403
TaqI	TCGA	5 cut(s)	129, 601, 817, 883, 936
TasI	AATT	3 cut(s)	121, 339, 735
TatI	WGTACW	1 cut(s)	804
TfiI	GAWTC	1 cut(s)	647
Tru1I	TTAA	2 cut(s)	120, 372
Tru9I	TTAA	2 cut(s)	120, 372
TscAI	CASTG	2 cut(s)	485, 814
TseFI	GTSAC	3 cut(s)	208, 560, 604
TseI	GCWGC	6 cut(s)	356, 446, 449, 764, 812, 914
Tsp45I	GTSAC	3 cut(s)	208, 560, 604
TspDTI	ATGAA	3 cut(s)	11, 39, 115
TspRI	CASTG	2 cut(s)	485, 814
Vha464I	CTTAAG	1 cut(s)	371
VpaK11BI	GGWCC	2 cut(s)	35, 221
XceI	RCATGY	2 cut(s)	687, 929
XhoI	CTCGAG	3 cut(s)	600, 816, 935
XmiI	GTMKAC	2 cut(s)	135, 576

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.