Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001661

Physical Location & Seq

Genomic Coordinates Reverse (-)

31936132 .. 31939051

Gene Structure 2920 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1251000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGGTCAAAGCCATTCGAGTCCATGAACTTGGCGGTCCTGAGGTGTTGAAATGGGAAGATGTGGAAGTAGAGGAGCCAAAGGAGGGTGAGATTCGCGTGAAGAACAAGGCTATCGGGCTTAATTTCATCGATGTACACTTCCGCCAAGGCATTTACAAGGCTCCTACGCTTCCCTACACCCCAGGTGTCGAGGCTTGTGGAGTTGTGACGGCTGTGGGACCTGGACTAACAGGCAGGCAAGTTGGAGATATAGTAGCAGTAGCATATGCTGGTCAGCCAATGGGCTCATATGCGGAAGAGCAGATCCTTCCTGCAAACAGAGCTGTGCCTGTTCCTGCTTCCATTGATCCTACAGTCGCAGCATCCCTTTTGCTTAAGGGTATGACAACTCAGTATCTAGTGCGCCGTTGTTTCAAGGTCGAACCTGGACACACAGTCCTTGTTCATGCAGCAGCTGGTGGAGTCGGATCTCTTCTATGCCAGTGGGCTAATGCCCTTGGTGCCACTGTGATTGGAACTGTATCAACTAAAGAGAAGGCAGCTCAAGCCAAGGAGGATGGATGCCACCATGTCATAATCTATAAGGAAGAGGACTTTGTTGCTCGTGTGAAAGAAATAACATCCGACAAAGGGGTTGAGGTTGTCTACGATTCTGTTGGGAAGGATACATTTGAGGGATCACTAGCGGTCTTAAAACCTCGGGGATACATGGTGAGTTTTGGGCAGTCATCAGGCGTGCCTGATCCAGTTTCATTACCAGCTCTTCAAGCGAAATCACTCTTCTTGACAAGGCCTACCCTCTTTCATTACACTGTTACGCAAGACGAATTGTTAGAGACTGCAGGAGATGTATTTGCTAATGTTGCATCGGGTGTACTGCGTGTTCGAGTGAATCACACCTACTCGTTATCTGAAGCAGCACAGGCACACGCAGACCTCGAGAACAGGAGAACATCTGGATCTGTTGTGCTTATCCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003960 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009653 GO:0009664 GO:0009826 GO:0009827 GO:0009828 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016491 GO:0016651 GO:0016655 GO:0017091 GO:0030312 GO:0032502 GO:0032989 GO:0033554 GO:0034599 GO:0040007 GO:0042221 GO:0042545 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051716 GO:0055114 GO:0060560 GO:0070887 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

327

Amino Acids

34.81

Weight (kDa)

6.01

Isoelectric Point (pI)

33.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	28 - 112	4.1e-15	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	154 - 276	2.4e-26	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	187 - 324	2.5e-22	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61510 AT5G61510
fragaria_vesca	FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27140
malus_domestica	MD01G1178400.v1.1 MD01G1179500.v1.1 MD01G1180600.v1.1 MD07G1250800.v1.1 MD07G1251000.v1.1
prunus_persica	Prupe.2G133300_v2.0.a1 Prupe.2G133300_v2.0.a1 Prupe.2G274500_v2.0.a1 Prupe.2G274600_v2.0.a1
pyrus_communis	pycom01g18920 pycom01g19010 pycom01g19080 pycom07g22370
rosa_chinensis	RchiOBHm_Chr1g0363511 RchiOBHm_Chr1g0373931 RchiOBHm_Chr1g0373941
rosa_laevigata	RLG00000026793 RLG00000026794 RLG00000027560
rosa_multiflora	Rmu_sc0002022.1_g000001 Rmu_sc0002740.1_g000012 Rmu_sc0008820.1_g000007
rosa_roxburghii	Rroxscaffold_4G00283890 Rroxscaffold_4G00293100
rosa_rugosa	Rorug01G0308000 Rorug01G0379800 Rorug01G0379900
rosa_samantha	Rh1AG316700 Rh1AG390100 Rh1AG390200 Rh1BG280100 Rh1BG354400 Rh1BG354500 Rh1CG296200 Rh1CG367500 Rh1CG367600 Rh1DG311300 Rh1DG385100 Rh1DG385200
rosa_wichuraiana	Rw1G028070 Rw1G034500 Rw1G034510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	500
AccI	GTMKAC	1 cut(s)	645
AccII	CGCG	1 cut(s)	96
AciI	CCGC	4 cut(s)	33, 142, 293, 686
AclWI	GGATC	6 cut(s)	298, 341, 475, 685, 737, 967
AcuI	CTGAAG	1 cut(s)	933
AfaI	GTAC	2 cut(s)	135, 876
AfiI	CCNNNNNNNGG	2 cut(s)	83, 630
AflII	CTTAAG	1 cut(s)	374
AgsI	TTSAA	3 cut(s)	49, 415, 767
AhdI	GACNNNNNGTC	1 cut(s)	434
AjnI	CCWGG	3 cut(s)	181, 220, 424
AluBI	AGCT	4 cut(s)	323, 455, 542, 761
AluI	AGCT	4 cut(s)	323, 455, 542, 761
Alw26I	GTCTC	1 cut(s)	830
AlwI	GGATC	6 cut(s)	298, 341, 475, 685, 737, 967
AlwNI	CAGNNNCTG	1 cut(s)	455
Ama87I	CYCGRG	2 cut(s)	699, 938
AoxI	GGCC	1 cut(s)	791
ApeKI	GCWGC	5 cut(s)	359, 449, 452, 539, 917
AspLEI	GCGC	1 cut(s)	405
AspS9I	GGNCC	2 cut(s)	35, 218
AsuHPI	GGTGA	2 cut(s)	98, 724
AvaI	CYCGRG	2 cut(s)	699, 938
AvaII	GGWCC	2 cut(s)	35, 218
AxyI	CCTNAGG	1 cut(s)	39
BanI	GGYRCC	1 cut(s)	500
BanII	GRGCYC	1 cut(s)	287
BauI	CACGAG	1 cut(s)	603
BbvI	GCAGC	5 cut(s)	371, 461, 464, 551, 929
BccI	CCATC	1 cut(s)	551
BceAI	ACGGC	2 cut(s)	225, 390
BciT130I	CCWGG	3 cut(s)	183, 222, 426
BciVI	GTATCC	2 cut(s)	658, 698
BcoDI	GTCTC	1 cut(s)	830
BfaI	CTAG	3 cut(s)	398, 683, 979
BfmI	CTRYAG	2 cut(s)	351, 840
BfrI	CTTAAG	1 cut(s)	374
BfuI	GTATCC	2 cut(s)	658, 698
BisI	GCNGC	5 cut(s)	360, 450, 453, 540, 918
BlsI	GCNGC	5 cut(s)	361, 451, 454, 541, 919
Bme1390I	CCNGG	3 cut(s)	183, 222, 426
Bme18I	GGWCC	2 cut(s)	35, 218
BmeRI	GACNNNNNGTC	1 cut(s)	434
BmeT110I	CYCGRG	2 cut(s)	699, 938
BmgT120I	GGNCC	2 cut(s)	35, 218
BmiI	GGNNCC	4 cut(s)	75, 162, 219, 502
BmrFI	CCNGG	3 cut(s)	183, 222, 426
BmsI	GCATC	3 cut(s)	371, 551, 875
BpuEI	CTTGAG	1 cut(s)	528
Bsa29I	ATCGAT	1 cut(s)	129
BsaJI	CCNNGG	5 cut(s)	145, 181, 496, 549, 698
BsaXI	ACNNNNNCTCC	2 cut(s)	237, 267
Bsc4I	CCNNNNNNNGG	2 cut(s)	83, 630
Bse1I	ACTGG	2 cut(s)	481, 746
Bse21I	CCTNAGG	1 cut(s)	39
BseBI	CCWGG	3 cut(s)	183, 222, 426
BseCI	ATCGAT	1 cut(s)	129
BseDI	CCNNGG	5 cut(s)	145, 181, 496, 549, 698
BseGI	GGATG	4 cut(s)	362, 562, 566, 620
BseLI	CCNNNNNNNGG	2 cut(s)	83, 630
BseMII	CTCAG	2 cut(s)	30, 404
BseNI	ACTGG	2 cut(s)	481, 746
BseRI	GAGGAG	1 cut(s)	86
BseXI	GCAGC	5 cut(s)	371, 461, 464, 551, 929
Bsh1236I	CGCG	1 cut(s)	96
BshFI	GGCC	1 cut(s)	793
BshNI	GGYRCC	1 cut(s)	500
BshVI	ATCGAT	1 cut(s)	129
BsiHKCI	CYCGRG	2 cut(s)	699, 938
BslFI	GGGAC	1 cut(s)	231
BslI	CCNNNNNNNGG	2 cut(s)	83, 630
BsmAI	GTCTC	1 cut(s)	830
BsmFI	GGGAC	1 cut(s)	231
BsnI	GGCC	1 cut(s)	793
BsoBI	CYCGRG	2 cut(s)	699, 938
Bsp1286I	GDGCHC	1 cut(s)	287
Bsp1407I	TGTACA	1 cut(s)	133
Bsp143I	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
BspACI	CCGC	4 cut(s)	33, 142, 293, 686
BspANI	GGCC	1 cut(s)	793
BspCNI	CTCAG	2 cut(s)	31, 403
BspDI	ATCGAT	1 cut(s)	129
BspFNI	CGCG	1 cut(s)	96
BspLI	GGNNCC	4 cut(s)	75, 162, 219, 502
BspMAI	CTGCAG	1 cut(s)	844
BspPI	GGATC	6 cut(s)	298, 341, 475, 685, 737, 967
BspQI	GCTCTTC	2 cut(s)	291, 768
BspT107I	GGYRCC	1 cut(s)	500
BspTI	CTTAAG	1 cut(s)	374
BsrGI	TGTACA	1 cut(s)	133
BsrI	ACTGG	2 cut(s)	481, 746
BssECI	CCNNGG	5 cut(s)	145, 181, 496, 549, 698
BssMI	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
BssSI	CACGAG	1 cut(s)	603
BssT1I	CCWWGG	3 cut(s)	145, 496, 549
Bst2BI	CACGAG	1 cut(s)	603
Bst2UI	CCWGG	3 cut(s)	183, 222, 426
Bst4CI	ACNGT	5 cut(s)	355, 436, 508, 520, 814
Bst6I	CTCTTC	5 cut(s)	291, 477, 582, 768, 785
BstAFI	CTTAAG	1 cut(s)	374
BstAUI	TGTACA	1 cut(s)	133
BstC8I	GCNNGC	2 cut(s)	236, 737
BstDEI	CTNAG	2 cut(s)	39, 390
BstF5I	GGATG	4 cut(s)	362, 562, 566, 620
BstFNI	CGCG	1 cut(s)	96
BstHHI	GCGC	1 cut(s)	405
BstKTI	GATC	6 cut(s)	306, 349, 470, 680, 745, 962
BstMAI	GTCTC	1 cut(s)	830
BstMBI	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
BstMWI	GCNNNNNNNGC	6 cut(s)	320, 500, 545, 767, 863, 923
BstNI	CCWGG	3 cut(s)	183, 222, 426
BstSCI	CCNGG	3 cut(s)	181, 220, 424
BstSFI	CTRYAG	2 cut(s)	351, 840
BstUI	CGCG	1 cut(s)	96
BstV1I	GCAGC	5 cut(s)	371, 461, 464, 551, 929
BstX2I	RGATCY	3 cut(s)	303, 467, 959
BstXI	CCANNNNNNTGG	1 cut(s)	29
BstYI	RGATCY	3 cut(s)	303, 467, 959
Bsu15I	ATCGAT	1 cut(s)	129
Bsu36I	CCTNAGG	1 cut(s)	39
BsuI	GTATCC	2 cut(s)	658, 698
BsuRI	GGCC	1 cut(s)	793
BsuTUI	ATCGAT	1 cut(s)	129
BtsCI	GGATG	4 cut(s)	362, 562, 566, 620
BtsIMutI	CAGTG	3 cut(s)	488, 504, 810
Cac8I	GCNNGC	2 cut(s)	236, 737
CaiI	CAGNNNCTG	1 cut(s)	455
CfoI	GCGC	1 cut(s)	405
Cfr13I	GGNCC	2 cut(s)	35, 218
ClaI	ATCGAT	1 cut(s)	129
Csp6I	GTAC	2 cut(s)	134, 875
CspCI	CAANNNNNGTGG	2 cut(s)	493, 528
CviAII	CATG	4 cut(s)	23, 446, 569, 709
CviQI	GTAC	2 cut(s)	134, 875
DdeI	CTNAG	2 cut(s)	39, 390
DpnI	GATC	6 cut(s)	305, 348, 469, 679, 744, 961
DpnII	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
DriI	GACNNNNNGTC	1 cut(s)	434
Eam1104I	CTCTTC	5 cut(s)	291, 477, 582, 768, 785
Eam1105I	GACNNNNNGTC	1 cut(s)	434
EarI	CTCTTC	5 cut(s)	291, 477, 582, 768, 785
EciI	GGCGGA	1 cut(s)	131
Eco130I	CCWWGG	3 cut(s)	145, 496, 549
Eco147I	AGGCCT	1 cut(s)	793
Eco24I	GRGCYC	1 cut(s)	287
Eco47I	GGWCC	2 cut(s)	35, 218
Eco57I	CTGAAG	1 cut(s)	933
Eco81I	CCTNAGG	1 cut(s)	39
Eco88I	CYCGRG	2 cut(s)	699, 938
EcoO109I	RGGNCCY	1 cut(s)	218
EcoRII	CCWGG	3 cut(s)	181, 220, 424
EcoT14I	CCWWGG	3 cut(s)	145, 496, 549
EcoT38I	GRGCYC	1 cut(s)	287
ErhI	CCWWGG	3 cut(s)	145, 496, 549
FaeI	CATG	4 cut(s)	26, 449, 572, 712
FaqI	GGGAC	1 cut(s)	231
FatI	CATG	4 cut(s)	22, 445, 568, 708
FauNDI	CATATG	2 cut(s)	265, 289
FblI	GTMKAC	1 cut(s)	645
Fnu4HI	GCNGC	5 cut(s)	360, 450, 453, 540, 918
FokI	GGATG	4 cut(s)	349, 569, 573, 607
FriOI	GRGCYC	1 cut(s)	287
Fsp4HI	GCNGC	5 cut(s)	360, 450, 453, 540, 918
FspBI	CTAG	3 cut(s)	398, 683, 979
GlaI	GCGC	1 cut(s)	404
GluI	GCNGC	5 cut(s)	360, 450, 453, 540, 918
HaeIII	GGCC	1 cut(s)	793
HhaI	GCGC	1 cut(s)	405
Hin1II	CATG	4 cut(s)	26, 449, 572, 712
Hin6I	GCGC	1 cut(s)	403
HinP1I	GCGC	1 cut(s)	403
HinfI	GANTC	5 cut(s)	18, 91, 462, 650, 892
HphI	GGTGA	2 cut(s)	98, 724
Hpy166II	GTNNAC	3 cut(s)	136, 646, 875
Hpy188I	TCNGA	3 cut(s)	467, 625, 913
Hpy188III	TCNNGA	4 cut(s)	38, 784, 940, 957
Hpy8I	GTNNAC	3 cut(s)	136, 646, 875
HpyAV	CCTTC	3 cut(s)	317, 529, 655
HpyCH4III	ACNGT	5 cut(s)	355, 436, 508, 520, 814
HpyCH4V	TGCA	4 cut(s)	314, 449, 842, 866
HpyF10VI	GCNNNNNNNGC	6 cut(s)	320, 500, 545, 767, 863, 923
HpyF3I	CTNAG	2 cut(s)	39, 390
Hsp92II	CATG	4 cut(s)	26, 449, 572, 712
HspAI	GCGC	1 cut(s)	403
Kzo9I	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
LguI	GCTCTTC	2 cut(s)	291, 768
LmnI	GCTCC	2 cut(s)	73, 166
Lsp1109I	GCAGC	5 cut(s)	371, 461, 464, 551, 929
LweI	GCATC	3 cut(s)	371, 551, 875
MaeI	CTAG	3 cut(s)	398, 683, 979
MaeIII	GTNAC	2 cut(s)	205, 814
MalI	GATC	6 cut(s)	305, 348, 469, 679, 744, 961
MboI	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
MboII	GAAGA	7 cut(s)	68, 112, 308, 464, 599, 755, 772
MflI	RGATCY	3 cut(s)	303, 467, 959
MhlI	GDGCHC	1 cut(s)	287
MluCI	AATT	2 cut(s)	121, 827
MlyI	GAGTC	2 cut(s)	27, 471
MmeI	TCCRAC	3 cut(s)	223, 445, 648
MseI	TTAA	3 cut(s)	120, 375, 692
MspA1I	CMGCKG	1 cut(s)	455
MspCI	CTTAAG	1 cut(s)	374
MspR9I	CCNGG	3 cut(s)	183, 222, 426
MvaI	CCWGG	3 cut(s)	183, 222, 426
MvnI	CGCG	1 cut(s)	96
MwoI	GCNNNNNNNGC	6 cut(s)	320, 500, 545, 767, 863, 923
NdeI	CATATG	2 cut(s)	265, 289
NdeII	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
NlaIII	CATG	4 cut(s)	26, 449, 572, 712
NlaIV	GGNNCC	4 cut(s)	75, 162, 219, 502
NmuCI	GTSAC	1 cut(s)	205
PaeR7I	CTCGAG	1 cut(s)	938
PceI	AGGCCT	1 cut(s)	793
PciSI	GCTCTTC	2 cut(s)	291, 768
PcsI	WCGNNNNNNNCGW	1 cut(s)	936
PfeI	GAWTC	3 cut(s)	91, 650, 892
PkrI	GCNGC	5 cut(s)	361, 451, 454, 541, 919
PleI	GAGTC	2 cut(s)	26, 470
PpsI	GAGTC	2 cut(s)	26, 470
PpuMI	RGGWCCY	1 cut(s)	218
Psp5II	RGGWCCY	1 cut(s)	218
Psp6I	CCWGG	3 cut(s)	181, 220, 424
PspGI	CCWGG	3 cut(s)	181, 220, 424
PspN4I	GGNNCC	4 cut(s)	75, 162, 219, 502
PspPI	GGNCC	2 cut(s)	35, 218
PspPPI	RGGWCCY	1 cut(s)	218
PsrI	GAACNNNNNNTAC	2 cut(s)	867, 899
PstI	CTGCAG	1 cut(s)	844
PstNI	CAGNNNCTG	1 cut(s)	455
PsuI	RGATCY	3 cut(s)	303, 467, 959
PvuII	CAGCTG	1 cut(s)	455
RsaI	GTAC	2 cut(s)	135, 876
RsaNI	GTAC	2 cut(s)	134, 875
SapI	GCTCTTC	2 cut(s)	291, 768
SaqAI	TTAA	3 cut(s)	120, 375, 692
SatI	GCNGC	5 cut(s)	360, 450, 453, 540, 918
Sau3AI	GATC	6 cut(s)	303, 346, 467, 677, 742, 959
Sau96I	GGNCC	2 cut(s)	35, 218
SchI	GAGTC	2 cut(s)	27, 471
ScrFI	CCNGG	3 cut(s)	183, 222, 426
SduI	GDGCHC	1 cut(s)	287
SfaNI	GCATC	3 cut(s)	371, 551, 875
SfcI	CTRYAG	2 cut(s)	351, 840
Sfr274I	CTCGAG	1 cut(s)	938
SinI	GGWCC	2 cut(s)	35, 218
SlaI	CTCGAG	1 cut(s)	938
SmlI	CTYRAG	3 cut(s)	374, 543, 938
SmoI	CTYRAG	3 cut(s)	374, 543, 938
Sse9I	AATT	2 cut(s)	121, 827
SseBI	AGGCCT	1 cut(s)	793
SsiI	CCGC	4 cut(s)	33, 142, 293, 686
SspMI	CTAG	3 cut(s)	398, 683, 979
StuI	AGGCCT	1 cut(s)	793
StyD4I	CCNGG	3 cut(s)	181, 220, 424
StyI	CCWWGG	3 cut(s)	145, 496, 549
TaaI	ACNGT	5 cut(s)	355, 436, 508, 520, 814
TaqI	TCGA	6 cut(s)	16, 129, 189, 420, 886, 939
TasI	AATT	2 cut(s)	121, 827
TatI	WGTACW	2 cut(s)	133, 874
TfiI	GAWTC	3 cut(s)	91, 650, 892
Tru1I	TTAA	3 cut(s)	120, 375, 692
Tru9I	TTAA	3 cut(s)	120, 375, 692
TscAI	CASTG	3 cut(s)	488, 511, 817
TseFI	GTSAC	1 cut(s)	205
TseI	GCWGC	5 cut(s)	359, 449, 452, 539, 917
Tsp45I	GTSAC	1 cut(s)	205
TspDTI	ATGAA	5 cut(s)	39, 115, 434, 741, 794
TspRI	CASTG	3 cut(s)	488, 511, 817
Vha464I	CTTAAG	1 cut(s)	374
VpaK11BI	GGWCC	2 cut(s)	35, 218
XhoI	CTCGAG	1 cut(s)	938
XmiI	GTMKAC	1 cut(s)	645
XspI	CTAG	3 cut(s)	398, 683, 979

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.