Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001661

Physical Location & Seq

Genomic Coordinates Reverse (-)

27991803 .. 27995546

Gene Structure 3744 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1180600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGGTTTACTTCCAATTAGCATCCAGAACCGCGGTTTAAGTTTAACCCCTTTCTTAATCACAAGTCCACCGCCCTATTTCCAGTTGCTGTCGGCTCCAAAATTCACACATTTTTCACAGATTTCCGGCAACAATTTCATCAATTGTTTACCTTCCAGGACCCAGAGATCATCAGTAGCAGTTAGATCAGTTGCCACTGAAGCAGCAGCATCACCACCAGCAGCAAAGATGGTCAAAGCCATCCGAATCCATGAACATGGTGGCCCTGAGGTGTTGAAATGGGAAGATGTGGAAGTAGGGGAGCCAAAGGAGGGCGAGATTCGCGTCAAGAATGAGGCCATCGGGATTAATTTCATTGATGTGTACTACCGCAAGGGCCTTTATAAGGCTGCTACGCTTCCCTACACCCCAGGTGTCGAGGCTTGTGGAGTCGTGACAGCTGTGGGACCAGGTCTAACGGGCAGGCAAGTTGGAGATCTTGTAGCCTATGCTGGTCAGCAAATGGGCTCATATGCGCAAGAGCAGATCCTTCCTGCAAACAGAGCTGTGCCTGTTCCTCCTTCCATTGATCCTAAAGTTGCAGCCTCCCTCATGCTTAAGGGTACGACAGCTCAGTTTCTACTACGCCGCTGTTTCAAGGTCGAACCTGGACACACAGTCCTTGTTCATGCAGCAGCTGGTGGAGTTGGATCCCTTTTATGCCAGTGGGCTAATGCCCTTGGTGCGACTGTCATTGGGACTGTATCAACTAAAGAAAAGGCAGTTCAGGCCAAGGAGGATGGATGCCACCACGTCATAATATATAAGGACGAGGACTTTGTCGCTCGTGTGAAAGAAATAACATCCAACAATGGGGTTGAAGTTGTCTACGATTCTGTAGGGAAGGATACGTTTGAGGGATCACTGGAAGTCTTAAAACTTCGGGGATATATGGTGAATTTTGGGCAGTCATCAGGTGCGCCTGATCCGGTTCCATTATCAGCTATTGCAGCGAAATCACTCTTCTTGACTCGGCCTAGCCTCTTTCATTACGCTGCAACTCGAGAGGAATTGTTAGAGGCCGCAGGAGAGGTATTTGCTAATGTTGCATCGGGTATATTGCGGGTTCGAGTGAATCACTCCTACTCATTATCTGAAGCAGCACAGGCACACACAGACCTTGAGAACAGGAAAACATCTGGATCTATCGTTCTTATCCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003960 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009653 GO:0009664 GO:0009826 GO:0009827 GO:0009828 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016491 GO:0016651 GO:0016655 GO:0017091 GO:0030312 GO:0032502 GO:0032989 GO:0033554 GO:0034599 GO:0040007 GO:0042221 GO:0042545 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051716 GO:0055114 GO:0060560 GO:0070887 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

401

Amino Acids

42.74

Weight (kDa)

8.18

Isoelectric Point (pI)

36.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	104 - 186	2.7e-14	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	228 - 337	4.9e-24	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	261 - 398	1.4e-19	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61510 AT5G61510
fragaria_vesca	FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27140
malus_domestica	MD01G1178400.v1.1 MD01G1179500.v1.1 MD01G1180600.v1.1 MD07G1250800.v1.1 MD07G1251000.v1.1
prunus_persica	Prupe.2G133300_v2.0.a1 Prupe.2G133300_v2.0.a1 Prupe.2G274500_v2.0.a1 Prupe.2G274600_v2.0.a1
pyrus_communis	pycom01g18920 pycom01g19010 pycom01g19080 pycom07g22370
rosa_chinensis	RchiOBHm_Chr1g0363511 RchiOBHm_Chr1g0373931 RchiOBHm_Chr1g0373941
rosa_laevigata	RLG00000026793 RLG00000026794 RLG00000027560
rosa_multiflora	Rmu_sc0002022.1_g000001 Rmu_sc0002740.1_g000012 Rmu_sc0008820.1_g000007
rosa_roxburghii	Rroxscaffold_4G00283890 Rroxscaffold_4G00293100
rosa_rugosa	Rorug01G0308000 Rorug01G0379800 Rorug01G0379900
rosa_samantha	Rh1AG316700 Rh1AG390100 Rh1AG390200 Rh1BG280100 Rh1BG354400 Rh1BG354500 Rh1CG296200 Rh1CG367500 Rh1CG367600 Rh1DG311300 Rh1DG385100 Rh1DG385200
rosa_wichuraiana	Rw1G028070 Rw1G034500 Rw1G034510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	384
Acc16I	TGCGCA	1 cut(s)	516
AccI	GTMKAC	1 cut(s)	867
AccII	CGCG	2 cut(s)	33, 324
AciI	CCGC	7 cut(s)	31, 33, 71, 370, 628, 1062, 1102
AclWI	GGATC	7 cut(s)	520, 563, 684, 697, 907, 959, 1189
AcsI	RAATTY	2 cut(s)	101, 937
AcuI	CTGAAG	2 cut(s)	219, 1155
AfaI	GTAC	2 cut(s)	365, 604
AfiI	CCNNNNNNNGG	3 cut(s)	311, 385, 852
AflII	CTTAAG	1 cut(s)	596
AgsI	TTSAA	3 cut(s)	277, 637, 860
AhdI	GACNNNNNGTC	1 cut(s)	656
AjiI	CACGTC	1 cut(s)	793
AjnI	CCWGG	4 cut(s)	155, 409, 448, 646
AloI	GAACNNNNNNTCC	1 cut(s)	1173
AluBI	AGCT	5 cut(s)	440, 545, 611, 677, 983
AluI	AGCT	5 cut(s)	440, 545, 611, 677, 983
AlwI	GGATC	7 cut(s)	520, 563, 684, 697, 907, 959, 1189
AlwNI	CAGNNNCTG	2 cut(s)	88, 677
Ama87I	CYCGRG	1 cut(s)	1041
AoxI	GGCC	6 cut(s)	262, 336, 376, 768, 1013, 1059
ApoI	RAATTY	2 cut(s)	101, 937
ArsI	GACNNNNNNTTYG	2 cut(s)	800, 832
AseI	ATTAAT	1 cut(s)	348
AspLEI	GCGC	2 cut(s)	517, 961
AspS9I	GGNCC	4 cut(s)	159, 263, 376, 446
AsuHPI	GGTGA	2 cut(s)	204, 946
AvaI	CYCGRG	1 cut(s)	1041
AvaII	GGWCC	2 cut(s)	159, 446
AxyI	CCTNAGG	1 cut(s)	267
BamHI	GGATCC	1 cut(s)	689
BanII	GRGCYC	1 cut(s)	509
BauI	CACGAG	1 cut(s)	825
BccI	CCATC	4 cut(s)	223, 248, 347, 773
BciT130I	CCWGG	4 cut(s)	157, 411, 450, 648
BciVI	GTATCC	1 cut(s)	880
BfaI	CTAG	2 cut(s)	1017, 1201
BfmI	CTRYAG	1 cut(s)	876
BfrI	CTTAAG	1 cut(s)	596
BfuI	GTATCC	1 cut(s)	880
BglII	AGATCT	1 cut(s)	475
Bme1390I	CCNGG	4 cut(s)	157, 411, 450, 648
Bme18I	GGWCC	2 cut(s)	159, 446
BmeRI	GACNNNNNGTC	1 cut(s)	656
BmeT110I	CYCGRG	1 cut(s)	1041
BmgBI	CACGTC	1 cut(s)	793
BmgT120I	GGNCC	4 cut(s)	159, 263, 376, 446
BmiI	GGNNCC	6 cut(s)	96, 161, 303, 447, 691, 972
BmrFI	CCNGG	4 cut(s)	157, 411, 450, 648
BmsI	GCATC	4 cut(s)	30, 218, 773, 1097
BpuEI	CTTGAG	1 cut(s)	1181
BsaJI	CCNNGG	4 cut(s)	31, 409, 718, 771
BsaWI	WCCGGW	1 cut(s)	967
BsaXI	ACNNNNNCTCC	2 cut(s)	465, 495
Bsc4I	CCNNNNNNNGG	3 cut(s)	311, 385, 852
Bse1I	ACTGG	3 cut(s)	82, 703, 909
Bse21I	CCTNAGG	1 cut(s)	267
BseBI	CCWGG	4 cut(s)	157, 411, 450, 648
BseDI	CCNNGG	4 cut(s)	31, 409, 718, 771
BseGI	GGATG	5 cut(s)	21, 240, 784, 788, 842
BseLI	CCNNNNNNNGG	3 cut(s)	311, 385, 852
BseMII	CTCAG	2 cut(s)	258, 626
BseNI	ACTGG	3 cut(s)	82, 703, 909
Bsh1236I	CGCG	2 cut(s)	33, 324
BshFI	GGCC	6 cut(s)	264, 338, 378, 770, 1015, 1061
BsiHKCI	CYCGRG	1 cut(s)	1041
BsiSI	CCGG	2 cut(s)	126, 968
BslFI	GGGAC	2 cut(s)	459, 751
BslI	CCNNNNNNNGG	3 cut(s)	311, 385, 852
BsmFI	GGGAC	2 cut(s)	459, 751
BsnI	GGCC	6 cut(s)	264, 338, 378, 770, 1015, 1061
BsoBI	CYCGRG	1 cut(s)	1041
Bsp1286I	GDGCHC	1 cut(s)	509
Bsp143I	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
BspACI	CCGC	7 cut(s)	31, 33, 71, 370, 628, 1062, 1102
BspANI	GGCC	6 cut(s)	264, 338, 378, 770, 1015, 1061
BspCNI	CTCAG	2 cut(s)	259, 625
BspFNI	CGCG	2 cut(s)	33, 324
BspLI	GGNNCC	6 cut(s)	96, 161, 303, 447, 691, 972
BspPI	GGATC	7 cut(s)	520, 563, 684, 697, 907, 959, 1189
BspTI	CTTAAG	1 cut(s)	596
BsrI	ACTGG	3 cut(s)	82, 703, 909
BssECI	CCNNGG	4 cut(s)	31, 409, 718, 771
BssMI	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
BssSI	CACGAG	1 cut(s)	825
BssT1I	CCWWGG	2 cut(s)	718, 771
Bst2BI	CACGAG	1 cut(s)	825
Bst2UI	CCWGG	4 cut(s)	157, 411, 450, 648
Bst4CI	ACNGT	3 cut(s)	658, 730, 742
Bst6I	CTCTTC	1 cut(s)	1007
BstAFI	CTTAAG	1 cut(s)	596
BstC8I	GCNNGC	1 cut(s)	464
BstDEI	CTNAG	2 cut(s)	267, 612
BstDSI	CCRYGG	1 cut(s)	31
BstENI	CCTNNNNNAGG	1 cut(s)	383
BstF5I	GGATG	5 cut(s)	21, 240, 784, 788, 842
BstFNI	CGCG	2 cut(s)	33, 324
BstHHI	GCGC	2 cut(s)	517, 961
BstKTI	GATC	9 cut(s)	170, 188, 478, 528, 571, 692, 902, 967, 1184
BstMBI	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
BstMWI	GCNNNNNNNGC	8 cut(s)	200, 321, 542, 722, 767, 989, 1085, 1145
BstNI	CCWGG	4 cut(s)	157, 411, 450, 648
BstSCI	CCNGG	4 cut(s)	155, 409, 448, 646
BstSFI	CTRYAG	1 cut(s)	876
BstUI	CGCG	2 cut(s)	33, 324
BstX2I	RGATCY	4 cut(s)	475, 525, 689, 1181
BstXI	CCANNNNNNTGG	1 cut(s)	257
BstYI	RGATCY	4 cut(s)	475, 525, 689, 1181
Bsu36I	CCTNAGG	1 cut(s)	267
BsuI	GTATCC	1 cut(s)	880
BsuRI	GGCC	6 cut(s)	264, 338, 378, 770, 1015, 1061
BtgI	CCRYGG	1 cut(s)	31
BtrI	CACGTC	1 cut(s)	793
BtsCI	GGATG	5 cut(s)	21, 240, 784, 788, 842
BtsIMutI	CAGTG	3 cut(s)	195, 710, 902
Cac8I	GCNNGC	1 cut(s)	464
CaiI	CAGNNNCTG	2 cut(s)	88, 677
CfoI	GCGC	2 cut(s)	517, 961
Cfr13I	GGNCC	4 cut(s)	159, 263, 376, 446
Cfr42I	CCGCGG	1 cut(s)	34
CseI	GACGC	1 cut(s)	313
CsiI	ACCWGGT	1 cut(s)	448
Csp6I	GTAC	2 cut(s)	364, 603
CviAII	CATG	4 cut(s)	251, 257, 592, 668
CviQI	GTAC	2 cut(s)	364, 603
DdeI	CTNAG	2 cut(s)	267, 612
DpnI	GATC	9 cut(s)	169, 187, 477, 527, 570, 691, 901, 966, 1183
DpnII	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
DriI	GACNNNNNGTC	1 cut(s)	656
Eam1104I	CTCTTC	1 cut(s)	1007
Eam1105I	GACNNNNNGTC	1 cut(s)	656
EarI	CTCTTC	1 cut(s)	1007
Eco130I	CCWWGG	2 cut(s)	718, 771
Eco24I	GRGCYC	1 cut(s)	509
Eco47I	GGWCC	2 cut(s)	159, 446
Eco57I	CTGAAG	2 cut(s)	219, 1155
Eco81I	CCTNAGG	1 cut(s)	267
Eco88I	CYCGRG	1 cut(s)	1041
EcoNI	CCTNNNNNAGG	1 cut(s)	383
EcoO109I	RGGNCCY	2 cut(s)	159, 376
EcoRII	CCWGG	4 cut(s)	155, 409, 448, 646
EcoT14I	CCWWGG	2 cut(s)	718, 771
EcoT38I	GRGCYC	1 cut(s)	509
ErhI	CCWWGG	2 cut(s)	718, 771
FaeI	CATG	4 cut(s)	254, 260, 595, 671
FaqI	GGGAC	2 cut(s)	459, 751
FatI	CATG	4 cut(s)	250, 256, 591, 667
FauI	CCCGC	1 cut(s)	1095
FauNDI	CATATG	1 cut(s)	511
FblI	GTMKAC	1 cut(s)	867
FokI	GGATG	5 cut(s)	8, 227, 791, 795, 829
FriOI	GRGCYC	1 cut(s)	509
FspBI	CTAG	2 cut(s)	1017, 1201
FspI	TGCGCA	1 cut(s)	516
GlaI	GCGC	2 cut(s)	516, 960
HaeIII	GGCC	6 cut(s)	264, 338, 378, 770, 1015, 1061
HapII	CCGG	2 cut(s)	126, 968
HgaI	GACGC	1 cut(s)	313
HhaI	GCGC	2 cut(s)	517, 961
Hin1II	CATG	4 cut(s)	254, 260, 595, 671
Hin6I	GCGC	2 cut(s)	515, 959
HinP1I	GCGC	2 cut(s)	515, 959
HinfI	GANTC	6 cut(s)	246, 319, 429, 872, 1009, 1114
HpaII	CCGG	2 cut(s)	126, 968
HphI	GGTGA	2 cut(s)	204, 946
Hpy166II	GTNNAC	5 cut(s)	8, 68, 149, 364, 868
Hpy188I	TCNGA	2 cut(s)	245, 1135
Hpy188III	TCNNGA	7 cut(s)	25, 328, 343, 433, 1006, 1043, 1179
Hpy8I	GTNNAC	5 cut(s)	8, 68, 149, 364, 868
HpyAV	CCTTC	4 cut(s)	162, 539, 570, 877
HpyCH4III	ACNGT	3 cut(s)	658, 730, 742
HpyCH4IV	ACGT	2 cut(s)	792, 890
HpyCH4V	TGCA	6 cut(s)	536, 581, 671, 989, 1037, 1088
HpyF10VI	GCNNNNNNNGC	8 cut(s)	200, 321, 542, 722, 767, 989, 1085, 1145
HpyF3I	CTNAG	2 cut(s)	267, 612
HpySE526I	ACGT	2 cut(s)	792, 890
Hsp92II	CATG	4 cut(s)	254, 260, 595, 671
HspAI	GCGC	2 cut(s)	515, 959
KspI	CCGCGG	1 cut(s)	34
Kzo9I	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
LmnI	GCTCC	2 cut(s)	100, 301
LweI	GCATC	4 cut(s)	30, 218, 773, 1097
MabI	ACCWGGT	1 cut(s)	448
MaeI	CTAG	2 cut(s)	1017, 1201
MaeII	ACGT	2 cut(s)	792, 890
MaeIII	GTNAC	1 cut(s)	433
MalI	GATC	9 cut(s)	169, 187, 477, 527, 570, 691, 901, 966, 1183
MboI	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
MboII	GAAGA	2 cut(s)	296, 994
MfeI	CAATTG	1 cut(s)	142
MflI	RGATCY	4 cut(s)	475, 525, 689, 1181
MhlI	GDGCHC	1 cut(s)	509
MluCI	AATT	7 cut(s)	15, 101, 133, 142, 349, 937, 1049
MlyI	GAGTC	2 cut(s)	438, 1003
MmeI	TCCRAC	3 cut(s)	451, 667, 870
MseI	TTAA	6 cut(s)	38, 44, 56, 348, 597, 914
MslI	CAYNNNNRTG	2 cut(s)	255, 359
MspA1I	CMGCKG	4 cut(s)	33, 440, 630, 677
MspCI	CTTAAG	1 cut(s)	596
MspI	CCGG	2 cut(s)	126, 968
MspR9I	CCNGG	4 cut(s)	157, 411, 450, 648
MunI	CAATTG	1 cut(s)	142
MvaI	CCWGG	4 cut(s)	157, 411, 450, 648
MvnI	CGCG	2 cut(s)	33, 324
MwoI	GCNNNNNNNGC	8 cut(s)	200, 321, 542, 722, 767, 989, 1085, 1145
NdeI	CATATG	1 cut(s)	511
NdeII	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
NlaIII	CATG	4 cut(s)	254, 260, 595, 671
NlaIV	GGNNCC	6 cut(s)	96, 161, 303, 447, 691, 972
NmeAIII	GCCGAG	1 cut(s)	991
NmuCI	GTSAC	1 cut(s)	433
NsbI	TGCGCA	1 cut(s)	516
PaeR7I	CTCGAG	1 cut(s)	1041
PfeI	GAWTC	4 cut(s)	246, 319, 872, 1114
PflFI	GACNNNGTC	2 cut(s)	450, 818
PfoI	TCCNGGA	1 cut(s)	155
PleI	GAGTC	2 cut(s)	437, 1003
PpsI	GAGTC	2 cut(s)	437, 1003
PpuMI	RGGWCCY	1 cut(s)	159
PshBI	ATTAAT	1 cut(s)	348
PsiI	TTATAA	1 cut(s)	384
Psp5II	RGGWCCY	1 cut(s)	159
Psp6I	CCWGG	4 cut(s)	155, 409, 448, 646
PspGI	CCWGG	4 cut(s)	155, 409, 448, 646
PspN4I	GGNNCC	6 cut(s)	96, 161, 303, 447, 691, 972
PspPI	GGNCC	4 cut(s)	159, 263, 376, 446
PspPPI	RGGWCCY	1 cut(s)	159
PstNI	CAGNNNCTG	2 cut(s)	88, 677
PsuI	RGATCY	4 cut(s)	475, 525, 689, 1181
PsyI	GACNNNGTC	2 cut(s)	450, 818
PvuII	CAGCTG	2 cut(s)	440, 677
RsaI	GTAC	2 cut(s)	365, 604
RsaNI	GTAC	2 cut(s)	364, 603
RseI	CAYNNNNRTG	2 cut(s)	255, 359
SacII	CCGCGG	1 cut(s)	34
SaqAI	TTAA	6 cut(s)	38, 44, 56, 348, 597, 914
Sau3AI	GATC	9 cut(s)	167, 185, 475, 525, 568, 689, 899, 964, 1181
Sau96I	GGNCC	4 cut(s)	159, 263, 376, 446
SchI	GAGTC	2 cut(s)	438, 1003
ScrFI	CCNGG	4 cut(s)	157, 411, 450, 648
SduI	GDGCHC	1 cut(s)	509
SexAI	ACCWGGT	1 cut(s)	448
SfaNI	GCATC	4 cut(s)	30, 218, 773, 1097
SfcI	CTRYAG	1 cut(s)	876
Sfr274I	CTCGAG	1 cut(s)	1041
Sfr303I	CCGCGG	1 cut(s)	34
SgrBI	CCGCGG	1 cut(s)	34
SinI	GGWCC	2 cut(s)	159, 446
SlaI	CTCGAG	1 cut(s)	1041
SmiMI	CAYNNNNRTG	2 cut(s)	255, 359
SmlI	CTYRAG	3 cut(s)	596, 1041, 1160
SmoI	CTYRAG	3 cut(s)	596, 1041, 1160
Sse9I	AATT	7 cut(s)	15, 101, 133, 142, 349, 937, 1049
SsiI	CCGC	7 cut(s)	31, 33, 71, 370, 628, 1062, 1102
SspMI	CTAG	2 cut(s)	1017, 1201
StyD4I	CCNGG	4 cut(s)	155, 409, 448, 646
StyI	CCWWGG	2 cut(s)	718, 771
TaaI	ACNGT	3 cut(s)	658, 730, 742
TaiI	ACGT	2 cut(s)	795, 893
TaqI	TCGA	4 cut(s)	417, 642, 1042, 1108
TasI	AATT	7 cut(s)	15, 101, 133, 142, 349, 937, 1049
TatI	WGTACW	1 cut(s)	363
TauI	GCSGC	2 cut(s)	630, 1064
TfiI	GAWTC	4 cut(s)	246, 319, 872, 1114
Tru1I	TTAA	6 cut(s)	38, 44, 56, 348, 597, 914
Tru9I	TTAA	6 cut(s)	38, 44, 56, 348, 597, 914
TscAI	CASTG	3 cut(s)	202, 710, 909
TseFI	GTSAC	1 cut(s)	433
Tsp45I	GTSAC	1 cut(s)	433
TspDTI	ATGAA	5 cut(s)	127, 267, 343, 656, 1016
TspRI	CASTG	3 cut(s)	202, 710, 909
Tth111I	GACNNNGTC	2 cut(s)	450, 818
Vha464I	CTTAAG	1 cut(s)	596
VpaK11BI	GGWCC	2 cut(s)	159, 446
VspI	ATTAAT	1 cut(s)	348
XagI	CCTNNNNNAGG	1 cut(s)	383
XapI	RAATTY	2 cut(s)	101, 937
XcmI	CCANNNNNNNNNTGG	1 cut(s)	257
XhoI	CTCGAG	1 cut(s)	1041
XmiI	GTMKAC	1 cut(s)	867
XspI	CTAG	2 cut(s)	1017, 1201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.