Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001661

Physical Location & Seq

Genomic Coordinates Reverse (-)

31925443 .. 31928117

Gene Structure 2675 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1250800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 975 bp

ATGGTCAAAGCCATTCGAGTCCATGAACTTGGCGGTCCTGAGGTGTTGAAATGGCAAGATGTGGAAGTAGAGGAGCCAAAGGAGGGTGAGATTCGTGTGAAGAACAAGGCTATCGGGCTTAATTTCATCGATGTATACTTCCGCAAAGGCATTTACAAGGCTCCTACGCTTCCCTACACCCCAGGTGTCGAGGCTTGTGGAGTTGTGACGGCTGTGGGACCTGGACTAACAGGCAGGCAAGTTGGAGATATAGTAGCATATGCTGGTCAGCCAATGGGCTCATATGCGGAAGAGCAGATCCTTCCTGCAAACAGAGCTGTGCCTGTTCCTGCTTCCATTGATCCTACAGTCGCAGCATCCCTCTTGCTTAAGGGTATGACTGCTCAGTTTCTAGTACGCCGTTGTTTCAAGGTCGAACCTGGACACACAGTCCTTGTTCATGCAGCAGCTGGTGGAGTCGGATCCCTTCTATGCCAGTGGGCTAATGCCCTTGGTGCCACTGTCATTGGAACTGTCTCAACTAAAGAGAAGGCAGCTCAAGCCAAGGAGGATGGATGCCACCATGTCATAATCTATAAGGAAGAGGACTTTGTCGCTCGTGTGAAAGAAATAACATCCGACAAGGGGGTTGAGGTTGTCTACGATTCTGTTGGGAAGGATACATTTGAGGGATCACTAGCGGTCTTAAAACTTCAAGGATACATGGTGAGTTTTGGGCAGTCATCAGGCGTGCCTGATCCAGTTCCATTACCAGCTCTTCAAGCGAAATCACTCTTCTTGACAAGGCCTGGCCTCTTTCATTACACTGTTACTCAAGACGAATTGTTAGAGACTGCAGAAGATGTATTTGCTAATGTTGCATCGGGTGTACTGCGTGTTCGAGTGAATCACACCTACTCGTTATCTGAAGCAGCACAGGCACACGCAGACCTCGAGAACAGGAGAACATCTGGATCTGTTGTGCTTATCCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003960 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009653 GO:0009664 GO:0009826 GO:0009827 GO:0009828 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016491 GO:0016651 GO:0016655 GO:0017091 GO:0030312 GO:0032502 GO:0032989 GO:0033554 GO:0034599 GO:0040007 GO:0042221 GO:0042545 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051716 GO:0055114 GO:0060560 GO:0070887 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

325

Amino Acids

34.65

Weight (kDa)

5.94

Isoelectric Point (pI)

36.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	28 - 110	3.6e-14	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	152 - 256	8.2e-25	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	185 - 322	2.6e-21	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61510 AT5G61510
fragaria_vesca	FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27132 FvH4_7g27140
malus_domestica	MD01G1178400.v1.1 MD01G1179500.v1.1 MD01G1180600.v1.1 MD07G1250800.v1.1 MD07G1251000.v1.1
prunus_persica	Prupe.2G133300_v2.0.a1 Prupe.2G133300_v2.0.a1 Prupe.2G274500_v2.0.a1 Prupe.2G274600_v2.0.a1
pyrus_communis	pycom01g18920 pycom01g19010 pycom01g19080 pycom07g22370
rosa_chinensis	RchiOBHm_Chr1g0363511 RchiOBHm_Chr1g0373931 RchiOBHm_Chr1g0373941
rosa_laevigata	RLG00000026793 RLG00000026794 RLG00000027560
rosa_multiflora	Rmu_sc0002022.1_g000001 Rmu_sc0002740.1_g000012 Rmu_sc0008820.1_g000007
rosa_roxburghii	Rroxscaffold_4G00283890 Rroxscaffold_4G00293100
rosa_rugosa	Rorug01G0308000 Rorug01G0379800 Rorug01G0379900
rosa_samantha	Rh1AG316700 Rh1AG390100 Rh1AG390200 Rh1BG280100 Rh1BG354400 Rh1BG354500 Rh1CG296200 Rh1CG367500 Rh1CG367600 Rh1DG311300 Rh1DG385100 Rh1DG385200
rosa_wichuraiana	Rw1G028070 Rw1G034500 Rw1G034510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	494
AccI	GTMKAC	2 cut(s)	135, 639
AciI	CCGC	4 cut(s)	33, 142, 287, 680
AclWI	GGATC	7 cut(s)	292, 335, 456, 469, 679, 731, 961
AcuI	CTGAAG	1 cut(s)	927
AfaI	GTAC	2 cut(s)	396, 870
AfiI	CCNNNNNNNGG	2 cut(s)	83, 624
AflII	CTTAAG	1 cut(s)	368
AgsI	TTSAA	4 cut(s)	49, 409, 695, 761
AhdI	GACNNNNNGTC	1 cut(s)	428
AjnI	CCWGG	4 cut(s)	181, 220, 418, 787
AluBI	AGCT	4 cut(s)	317, 449, 536, 755
AluI	AGCT	4 cut(s)	317, 449, 536, 755
Alw26I	GTCTC	2 cut(s)	520, 824
AlwI	GGATC	7 cut(s)	292, 335, 456, 469, 679, 731, 961
AlwNI	CAGNNNCTG	1 cut(s)	449
Ama87I	CYCGRG	1 cut(s)	932
AoxI	GGCC	2 cut(s)	785, 790
ApeKI	GCWGC	5 cut(s)	353, 443, 446, 533, 911
AspS9I	GGNCC	2 cut(s)	35, 218
AsuHPI	GGTGA	2 cut(s)	98, 718
AvaI	CYCGRG	1 cut(s)	932
AvaII	GGWCC	2 cut(s)	35, 218
AxyI	CCTNAGG	1 cut(s)	39
BamHI	GGATCC	1 cut(s)	461
BanI	GGYRCC	1 cut(s)	494
BanII	GRGCYC	1 cut(s)	281
BauI	CACGAG	1 cut(s)	597
BbvI	GCAGC	5 cut(s)	365, 455, 458, 545, 923
BccI	CCATC	1 cut(s)	545
BceAI	ACGGC	2 cut(s)	225, 384
BciT130I	CCWGG	4 cut(s)	183, 222, 420, 789
BciVI	GTATCC	2 cut(s)	652, 692
BcoDI	GTCTC	2 cut(s)	520, 824
BfaI	CTAG	3 cut(s)	392, 677, 973
BfmI	CTRYAG	2 cut(s)	345, 834
BfrI	CTTAAG	1 cut(s)	368
BfuI	GTATCC	2 cut(s)	652, 692
BisI	GCNGC	5 cut(s)	354, 444, 447, 534, 912
BlsI	GCNGC	5 cut(s)	355, 445, 448, 535, 913
Bme1390I	CCNGG	4 cut(s)	183, 222, 420, 789
Bme18I	GGWCC	2 cut(s)	35, 218
BmeRI	GACNNNNNGTC	1 cut(s)	428
BmeT110I	CYCGRG	1 cut(s)	932
BmgT120I	GGNCC	2 cut(s)	35, 218
BmiI	GGNNCC	5 cut(s)	75, 162, 219, 463, 496
BmrFI	CCNGG	4 cut(s)	183, 222, 420, 789
BmsI	GCATC	3 cut(s)	365, 545, 869
BpuEI	CTTGAG	2 cut(s)	522, 798
Bsa29I	ATCGAT	1 cut(s)	129
BsaJI	CCNNGG	3 cut(s)	181, 490, 543
BsaXI	ACNNNNNCTCC	2 cut(s)	237, 267
Bsc4I	CCNNNNNNNGG	2 cut(s)	83, 624
Bse1I	ACTGG	2 cut(s)	475, 740
Bse21I	CCTNAGG	1 cut(s)	39
BseBI	CCWGG	4 cut(s)	183, 222, 420, 789
BseCI	ATCGAT	1 cut(s)	129
BseDI	CCNNGG	3 cut(s)	181, 490, 543
BseGI	GGATG	4 cut(s)	356, 556, 560, 614
BseLI	CCNNNNNNNGG	2 cut(s)	83, 624
BseMII	CTCAG	2 cut(s)	30, 398
BseNI	ACTGG	2 cut(s)	475, 740
BseRI	GAGGAG	1 cut(s)	86
BseXI	GCAGC	5 cut(s)	365, 455, 458, 545, 923
BshFI	GGCC	2 cut(s)	787, 792
BshNI	GGYRCC	1 cut(s)	494
BshVI	ATCGAT	1 cut(s)	129
BsiHKCI	CYCGRG	1 cut(s)	932
BslFI	GGGAC	1 cut(s)	231
BslI	CCNNNNNNNGG	2 cut(s)	83, 624
BsmAI	GTCTC	2 cut(s)	520, 824
BsmFI	GGGAC	1 cut(s)	231
BsnI	GGCC	2 cut(s)	787, 792
BsoBI	CYCGRG	1 cut(s)	932
Bsp1286I	GDGCHC	1 cut(s)	281
Bsp143I	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
BspACI	CCGC	4 cut(s)	33, 142, 287, 680
BspANI	GGCC	2 cut(s)	787, 792
BspCNI	CTCAG	2 cut(s)	31, 397
BspDI	ATCGAT	1 cut(s)	129
BspLI	GGNNCC	5 cut(s)	75, 162, 219, 463, 496
BspMAI	CTGCAG	1 cut(s)	838
BspPI	GGATC	7 cut(s)	292, 335, 456, 469, 679, 731, 961
BspQI	GCTCTTC	2 cut(s)	285, 762
BspT107I	GGYRCC	1 cut(s)	494
BspTI	CTTAAG	1 cut(s)	368
BsrI	ACTGG	2 cut(s)	475, 740
BssECI	CCNNGG	3 cut(s)	181, 490, 543
BssMI	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
BssNAI	GTATAC	1 cut(s)	136
BssSI	CACGAG	1 cut(s)	597
BssT1I	CCWWGG	2 cut(s)	490, 543
Bst1107I	GTATAC	1 cut(s)	136
Bst2BI	CACGAG	1 cut(s)	597
Bst2UI	CCWGG	4 cut(s)	183, 222, 420, 789
Bst4CI	ACNGT	5 cut(s)	349, 430, 502, 514, 808
Bst6I	CTCTTC	4 cut(s)	285, 576, 762, 779
BstAFI	CTTAAG	1 cut(s)	368
BstC8I	GCNNGC	2 cut(s)	236, 731
BstDEI	CTNAG	2 cut(s)	39, 384
BstF5I	GGATG	4 cut(s)	356, 556, 560, 614
BstKTI	GATC	6 cut(s)	300, 343, 464, 674, 739, 956
BstMAI	GTCTC	2 cut(s)	520, 824
BstMBI	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
BstMWI	GCNNNNNNNGC	6 cut(s)	314, 494, 539, 761, 857, 917
BstNI	CCWGG	4 cut(s)	183, 222, 420, 789
BstSCI	CCNGG	4 cut(s)	181, 220, 418, 787
BstSFI	CTRYAG	2 cut(s)	345, 834
BstV1I	GCAGC	5 cut(s)	365, 455, 458, 545, 923
BstX2I	RGATCY	3 cut(s)	297, 461, 953
BstXI	CCANNNNNNTGG	1 cut(s)	29
BstYI	RGATCY	3 cut(s)	297, 461, 953
BstZ17I	GTATAC	1 cut(s)	136
Bsu15I	ATCGAT	1 cut(s)	129
Bsu36I	CCTNAGG	1 cut(s)	39
BsuI	GTATCC	2 cut(s)	652, 692
BsuRI	GGCC	2 cut(s)	787, 792
BsuTUI	ATCGAT	1 cut(s)	129
BtsCI	GGATG	4 cut(s)	356, 556, 560, 614
BtsIMutI	CAGTG	3 cut(s)	482, 498, 804
Cac8I	GCNNGC	2 cut(s)	236, 731
CaiI	CAGNNNCTG	1 cut(s)	449
Cfr13I	GGNCC	2 cut(s)	35, 218
ClaI	ATCGAT	1 cut(s)	129
Csp6I	GTAC	2 cut(s)	395, 869
CspCI	CAANNNNNGTGG	2 cut(s)	487, 522
CviAII	CATG	4 cut(s)	23, 440, 563, 703
CviQI	GTAC	2 cut(s)	395, 869
DdeI	CTNAG	2 cut(s)	39, 384
DpnI	GATC	6 cut(s)	299, 342, 463, 673, 738, 955
DpnII	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
DriI	GACNNNNNGTC	1 cut(s)	428
Eam1104I	CTCTTC	4 cut(s)	285, 576, 762, 779
Eam1105I	GACNNNNNGTC	1 cut(s)	428
EarI	CTCTTC	4 cut(s)	285, 576, 762, 779
Eco130I	CCWWGG	2 cut(s)	490, 543
Eco147I	AGGCCT	1 cut(s)	787
Eco24I	GRGCYC	1 cut(s)	281
Eco47I	GGWCC	2 cut(s)	35, 218
Eco57I	CTGAAG	1 cut(s)	927
Eco81I	CCTNAGG	1 cut(s)	39
Eco88I	CYCGRG	1 cut(s)	932
EcoO109I	RGGNCCY	1 cut(s)	218
EcoRII	CCWGG	4 cut(s)	181, 220, 418, 787
EcoT14I	CCWWGG	2 cut(s)	490, 543
EcoT38I	GRGCYC	1 cut(s)	281
ErhI	CCWWGG	2 cut(s)	490, 543
FaeI	CATG	4 cut(s)	26, 443, 566, 706
FaqI	GGGAC	1 cut(s)	231
FatI	CATG	4 cut(s)	22, 439, 562, 702
FauNDI	CATATG	2 cut(s)	259, 283
FblI	GTMKAC	2 cut(s)	135, 639
Fnu4HI	GCNGC	5 cut(s)	354, 444, 447, 534, 912
FokI	GGATG	4 cut(s)	343, 563, 567, 601
FriOI	GRGCYC	1 cut(s)	281
Fsp4HI	GCNGC	5 cut(s)	354, 444, 447, 534, 912
FspBI	CTAG	3 cut(s)	392, 677, 973
GluI	GCNGC	5 cut(s)	354, 444, 447, 534, 912
HaeIII	GGCC	2 cut(s)	787, 792
Hin1II	CATG	4 cut(s)	26, 443, 566, 706
HinfI	GANTC	5 cut(s)	18, 91, 456, 644, 886
HphI	GGTGA	2 cut(s)	98, 718
Hpy166II	GTNNAC	3 cut(s)	136, 640, 869
Hpy188I	TCNGA	3 cut(s)	461, 619, 907
Hpy188III	TCNNGA	5 cut(s)	38, 778, 815, 934, 951
Hpy8I	GTNNAC	3 cut(s)	136, 640, 869
HpyAV	CCTTC	4 cut(s)	311, 476, 523, 649
HpyCH4III	ACNGT	5 cut(s)	349, 430, 502, 514, 808
HpyCH4V	TGCA	4 cut(s)	308, 443, 836, 860
HpyF10VI	GCNNNNNNNGC	6 cut(s)	314, 494, 539, 761, 857, 917
HpyF3I	CTNAG	2 cut(s)	39, 384
Hsp92II	CATG	4 cut(s)	26, 443, 566, 706
Kzo9I	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
LguI	GCTCTTC	2 cut(s)	285, 762
LmnI	GCTCC	2 cut(s)	73, 166
Lsp1109I	GCAGC	5 cut(s)	365, 455, 458, 545, 923
LweI	GCATC	3 cut(s)	365, 545, 869
MaeI	CTAG	3 cut(s)	392, 677, 973
MaeIII	GTNAC	2 cut(s)	205, 808
MalI	GATC	6 cut(s)	299, 342, 463, 673, 738, 955
MboI	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
MboII	GAAGA	6 cut(s)	112, 302, 593, 749, 766, 851
MflI	RGATCY	3 cut(s)	297, 461, 953
MhlI	GDGCHC	1 cut(s)	281
MluCI	AATT	2 cut(s)	121, 821
MlyI	GAGTC	2 cut(s)	27, 465
MmeI	TCCRAC	3 cut(s)	223, 439, 642
MseI	TTAA	3 cut(s)	120, 369, 686
MspA1I	CMGCKG	1 cut(s)	449
MspCI	CTTAAG	1 cut(s)	368
MspR9I	CCNGG	4 cut(s)	183, 222, 420, 789
MvaI	CCWGG	4 cut(s)	183, 222, 420, 789
MwoI	GCNNNNNNNGC	6 cut(s)	314, 494, 539, 761, 857, 917
NdeI	CATATG	2 cut(s)	259, 283
NdeII	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
NlaIII	CATG	4 cut(s)	26, 443, 566, 706
NlaIV	GGNNCC	5 cut(s)	75, 162, 219, 463, 496
NmuCI	GTSAC	1 cut(s)	205
PaeR7I	CTCGAG	1 cut(s)	932
PceI	AGGCCT	1 cut(s)	787
PciSI	GCTCTTC	2 cut(s)	285, 762
PcsI	WCGNNNNNNNCGW	1 cut(s)	930
PfeI	GAWTC	3 cut(s)	91, 644, 886
PflFI	GACNNNGTC	1 cut(s)	590
PkrI	GCNGC	5 cut(s)	355, 445, 448, 535, 913
PleI	GAGTC	2 cut(s)	26, 464
PpsI	GAGTC	2 cut(s)	26, 464
PpuMI	RGGWCCY	1 cut(s)	218
Psp5II	RGGWCCY	1 cut(s)	218
Psp6I	CCWGG	4 cut(s)	181, 220, 418, 787
PspGI	CCWGG	4 cut(s)	181, 220, 418, 787
PspN4I	GGNNCC	5 cut(s)	75, 162, 219, 463, 496
PspPI	GGNCC	2 cut(s)	35, 218
PspPPI	RGGWCCY	1 cut(s)	218
PsrI	GAACNNNNNNTAC	2 cut(s)	861, 893
PstI	CTGCAG	1 cut(s)	838
PstNI	CAGNNNCTG	1 cut(s)	449
PsuI	RGATCY	3 cut(s)	297, 461, 953
PsyI	GACNNNGTC	1 cut(s)	590
PvuII	CAGCTG	1 cut(s)	449
RsaI	GTAC	2 cut(s)	396, 870
RsaNI	GTAC	2 cut(s)	395, 869
SapI	GCTCTTC	2 cut(s)	285, 762
SaqAI	TTAA	3 cut(s)	120, 369, 686
SatI	GCNGC	5 cut(s)	354, 444, 447, 534, 912
Sau3AI	GATC	6 cut(s)	297, 340, 461, 671, 736, 953
Sau96I	GGNCC	2 cut(s)	35, 218
SchI	GAGTC	2 cut(s)	27, 465
ScrFI	CCNGG	4 cut(s)	183, 222, 420, 789
SduI	GDGCHC	1 cut(s)	281
SfaNI	GCATC	3 cut(s)	365, 545, 869
SfcI	CTRYAG	2 cut(s)	345, 834
Sfr274I	CTCGAG	1 cut(s)	932
SinI	GGWCC	2 cut(s)	35, 218
SlaI	CTCGAG	1 cut(s)	932
SmlI	CTYRAG	4 cut(s)	368, 537, 813, 932
SmoI	CTYRAG	4 cut(s)	368, 537, 813, 932
Sse9I	AATT	2 cut(s)	121, 821
SseBI	AGGCCT	1 cut(s)	787
SsiI	CCGC	4 cut(s)	33, 142, 287, 680
SspMI	CTAG	3 cut(s)	392, 677, 973
StuI	AGGCCT	1 cut(s)	787
StyD4I	CCNGG	4 cut(s)	181, 220, 418, 787
StyI	CCWWGG	2 cut(s)	490, 543
TaaI	ACNGT	5 cut(s)	349, 430, 502, 514, 808
TaqI	TCGA	6 cut(s)	16, 129, 189, 414, 880, 933
TasI	AATT	2 cut(s)	121, 821
TatI	WGTACW	1 cut(s)	868
TfiI	GAWTC	3 cut(s)	91, 644, 886
Tru1I	TTAA	3 cut(s)	120, 369, 686
Tru9I	TTAA	3 cut(s)	120, 369, 686
TscAI	CASTG	3 cut(s)	482, 505, 811
TseFI	GTSAC	1 cut(s)	205
TseI	GCWGC	5 cut(s)	353, 443, 446, 533, 911
Tsp45I	GTSAC	1 cut(s)	205
TspDTI	ATGAA	4 cut(s)	39, 115, 428, 788
TspRI	CASTG	3 cut(s)	482, 505, 811
Tth111I	GACNNNGTC	1 cut(s)	590
Vha464I	CTTAAG	1 cut(s)	368
VpaK11BI	GGWCC	2 cut(s)	35, 218
XhoI	CTCGAG	1 cut(s)	932
XmiI	GTMKAC	2 cut(s)	135, 639
XspI	CTAG	3 cut(s)	392, 677, 973

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.