MD01G1206400.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002046
Physical Location & Seq
Reverse (-)
30178376 .. 30184226
5851 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1206400.v1.1.491

Sequence Viewer

Length: 651 bp
ATGTCGAGCTCTCTGCTGTCTCTCTCTCCCTCAGTGTCATTTTCATCATCCCTTTTGGTTTCCTGCATGAATTTTTGGAATTGGGAAGTTATGTTGTTGCTTATGTTGTTGCTTGGAATAAATGCTGCTATAAGCACACGAGTCTCGAACGAACTTGGGATGGGGCGCCCGAGAGCAGCCAAATACTCCGTCTGCGTTGCAGTCTTGCAAGCTCTCATCGTCGGGGTTTTAGCCATGGTTGCTATCTTCATAAGCAGAGACTACAGTGCCATGATTTTCACCAGCAGTGGAGAGATGCAGCTCGCCGTTGCTCGTTTAGCATACCTTCTTGGTGTAACCATGCTTCTTAATTACCAGTGCTACACAAGAGTTGCTATTGGAGGTGGATGGCAAGTGATGGCGGCTTATATAAATTTTGGTAGTTATTTGGTTGGGCTCCCGTTAGCCATCTTTCTTGGCTTCAAAGCAAATCTTGGAGCTGTGGGACTTTGGGGTGGGATGATGAGTGGAAATGCTCTTCAGATCTTCTTCCTGCTGATTCTGACTTGTAGAATTAACTGGGACAGAGAGGTGGAGCAAACAACAAAGCGCATAAAGAAGTGGGGAAGCCAAGAAATAAAAACTGACAAGATATATAAAAATATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

217

Amino Acids

23.74

Weight (kDa)

9.51

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 25 - 150 1.5e-13 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 165
AciI CCGC 1 cut(s) 401
AcsI RAATTY 2 cut(s) 70, 412
AcuI CTGAAG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 166
AgsI TTSAA 1 cut(s) 463
AluBI AGCT 4 cut(s) 9, 212, 301, 479
AluI AGCT 4 cut(s) 9, 212, 301, 479
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 3 cut(s) 24, 148, 252
Ama87I CYCGRG 1 cut(s) 169
ApeKI GCWGC 3 cut(s) 125, 176, 298
ApoI RAATTY 2 cut(s) 70, 412
ArsI GACNNNNNNTTYG 2 cut(s) 174, 206
AspLEI GCGC 2 cut(s) 168, 591
AsuHPI GGTGA 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 169
BanI GGYRCC 1 cut(s) 165
BanII GRGCYC 2 cut(s) 11, 438
BarI GAAGNNNNNNTAC 2 cut(s) 327, 359
BauI CACGAG 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 112, 188, 310
BccI CCATC 4 cut(s) 154, 381, 391, 455
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 1 cut(s) 29
BcoDI GTCTC 3 cut(s) 24, 148, 252
BfmI CTRYAG 1 cut(s) 262
BfoI RGCGCY 1 cut(s) 169
BglII AGATCT 1 cut(s) 522
BisI GCNGC 4 cut(s) 126, 177, 299, 402
BlsI GCNGC 4 cut(s) 127, 178, 300, 403
BmeT110I CYCGRG 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 167, 437
BmrI ACTGGG 1 cut(s) 568
BmsI GCATC 1 cut(s) 285
BmuI ACTGGG 1 cut(s) 568
BsaHI GRCGYC 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 234
Bse1I ACTGG 2 cut(s) 355, 563
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 4 cut(s) 47, 165, 392, 504
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 2 cut(s) 355, 563
BseXI GCAGC 3 cut(s) 112, 188, 310
BshNI GGYRCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 169
BslFI GGGAC 2 cut(s) 498, 575
BsmAI GTCTC 3 cut(s) 24, 148, 252
BsmFI GGGAC 2 cut(s) 498, 575
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 11, 438
Bsp143I GATC 1 cut(s) 522
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 1 cut(s) 401
BspCNI CTCAG 1 cut(s) 44
BspHI TCATGA 1 cut(s) 647
BspLI GGNNCC 2 cut(s) 167, 437
BspQI GCTCTTC 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 165
BsrI ACTGG 2 cut(s) 355, 563
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 1 cut(s) 522
BssNI GRCGYC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 234
Bst2BI CACGAG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 522
BstACI GRCGYC 1 cut(s) 166
BstC8I GCNNGC 2 cut(s) 210, 303
BstDEI CTNAG 1 cut(s) 31
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 4 cut(s) 47, 165, 392, 504
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 2 cut(s) 168, 591
BstKTI GATC 1 cut(s) 525
BstMAI GTCTC 3 cut(s) 24, 148, 252
BstMBI GATC 1 cut(s) 522
BstMWI GCNNNNNNNGC 2 cut(s) 239, 317
BstSFI CTRYAG 1 cut(s) 262
BstV1I GCAGC 3 cut(s) 112, 188, 310
BstX2I RGATCY 1 cut(s) 522
BstYI RGATCY 1 cut(s) 522
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 4 cut(s) 47, 165, 392, 504
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 4 cut(s) 39, 271, 292, 362
Cac8I GCNNGC 2 cut(s) 210, 303
CciI TCATGA 1 cut(s) 647
CfoI GCGC 2 cut(s) 168, 591
CviAII CATG 5 cut(s) 67, 235, 271, 340, 648
DdeI CTNAG 1 cut(s) 31
DinI GGCGCC 1 cut(s) 167
DpnI GATC 1 cut(s) 524
DpnII GATC 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 522
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 2 cut(s) 11, 438
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 2 cut(s) 11, 438
EgeI GGCGCC 1 cut(s) 167
EheI GGCGCC 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 5 cut(s) 70, 238, 274, 343, 651
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 2 cut(s) 498, 575
FatI CATG 5 cut(s) 66, 234, 270, 339, 647
Fnu4HI GCNGC 4 cut(s) 126, 177, 299, 402
FokI GGATG 4 cut(s) 34, 172, 399, 511
FriOI GRGCYC 2 cut(s) 11, 438
Fsp4HI GCNGC 4 cut(s) 126, 177, 299, 402
GlaI GCGC 2 cut(s) 167, 590
GluI GCNGC 4 cut(s) 126, 177, 299, 402
HaeII RGCGCY 1 cut(s) 169
HhaI GCGC 2 cut(s) 168, 591
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 5 cut(s) 70, 238, 274, 343, 651
Hin6I GCGC 2 cut(s) 166, 589
HinP1I GCGC 2 cut(s) 166, 589
HinfI GANTC 2 cut(s) 141, 538
HphI GGTGA 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 522, 543
Hpy188III TCNNGA 2 cut(s) 145, 648
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4V TGCA 4 cut(s) 66, 200, 208, 298
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 317
HpyF3I CTNAG 1 cut(s) 31
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 5 cut(s) 70, 238, 274, 343, 651
HspAI GCGC 2 cut(s) 166, 589
KasI GGCGCC 1 cut(s) 165
Kzo9I GATC 1 cut(s) 522
LguI GCTCTTC 1 cut(s) 522
LmnI GCTCC 3 cut(s) 441, 476, 574
LpnPI CCDG 5 cut(s) 76, 295, 368, 544, 545
Lsp1109I GCAGC 3 cut(s) 112, 188, 310
LweI GCATC 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 334
MalI GATC 1 cut(s) 524
MboI GATC 1 cut(s) 522
MboII GAAGA 4 cut(s) 238, 509, 517, 520
MflI RGATCY 1 cut(s) 522
MhlI GDGCHC 2 cut(s) 11, 438
MluCI AATT 5 cut(s) 70, 79, 349, 412, 552
Mly113I GGCGCC 1 cut(s) 166
MlyI GAGTC 1 cut(s) 150
MnlI CCTC 3 cut(s) 40, 374, 562
MseI TTAA 2 cut(s) 348, 555
MwoI GCNNNNNNNGC 2 cut(s) 239, 317
NarI GGCGCC 1 cut(s) 166
NcoI CCATGG 1 cut(s) 234
NdeII GATC 1 cut(s) 522
NlaIII CATG 5 cut(s) 70, 238, 274, 343, 651
NlaIV GGNNCC 2 cut(s) 167, 437
PagI TCATGA 1 cut(s) 647
PciSI GCTCTTC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 538
PkrI GCNGC 4 cut(s) 127, 178, 300, 403
PleI GAGTC 1 cut(s) 149
PluTI GGCGCC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 167, 437
PsuI RGATCY 1 cut(s) 522
SacI GAGCTC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 522
SaqAI TTAA 2 cut(s) 348, 555
SatI GCNGC 4 cut(s) 126, 177, 299, 402
Sau3AI GATC 1 cut(s) 522
SchI GAGTC 1 cut(s) 150
SduI GDGCHC 2 cut(s) 11, 438
SetI ASST 7 cut(s) 11, 214, 303, 327, 385, 481, 573
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 262
SfoI GGCGCC 1 cut(s) 167
Sse9I AATT 5 cut(s) 70, 79, 349, 412, 552
SsiI CCGC 1 cut(s) 401
SspDI GGCGCC 1 cut(s) 165
SstI GAGCTC 1 cut(s) 11
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 266
TaqI TCGA 2 cut(s) 5, 146
TasI AATT 5 cut(s) 70, 79, 349, 412, 552
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 1 cut(s) 538
Tru1I TTAA 2 cut(s) 348, 555
Tru9I TTAA 2 cut(s) 348, 555
TscAI CASTG 4 cut(s) 39, 271, 292, 362
TseI GCWGC 3 cut(s) 125, 176, 298
TspDTI ATGAA 3 cut(s) 33, 83, 238
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 4 cut(s) 39, 271, 292, 362
XapI RAATTY 2 cut(s) 70, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.