Rh1BG384300

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002046
Physical Location & Seq
Reverse (-)
51240791 .. 51245755
4965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG384300.1

Sequence Viewer

Length: 546 bp
ATGGAAACACAGACAGTACCCTTGCTGATCATCAAACCTAAATCAGATGAGACTGAAGACTATGTGCCGGTGAGTAGCTTTGAGGAGGCCTTTTTGGTCTTTTGGAAAGAGACAGTGAAGCTGTGGAAGATTGCAGCTCCAGTTGCAATAACAATGTTGTTTCAGTACTTGATTCAATCTGTCACCACCATATTTGTAGGCCATCTCGGAGACCTTGAACTCTCTGCTGTTTCACTGGCTCACAATGTCATTGGCAACATCTCTTATGGTGTTGCTGTTGGAGGTGGATGGCAGGTGATGGTAGCTTATATAAATTTGGGTTGTTATTATTTGTTTGGTCTTCCACTTGCAATCCTTCTTGGGTTTAAAGCAAATCTAGGTGCCATGGGACTATGGGGTGGCATGATGAGTGGAAGTGCTCTTCAAATCCTGTTCCTACTGATTCTGACTTCTAGAACCAACTGGGACAATGAGGTGGAGCAAACCACAGAGCGCATAAGGAAGTGGGGGAACCAAGAAAATGAGACTCAGAATATAGCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

181

Amino Acids

20.07

Weight (kDa)

4.83

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 47 - 84 2.5e-06 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 283
Acc36I ACCTGC 1 cut(s) 283
AccB1I GGYRCC 1 cut(s) 380
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 2 cut(s) 18, 167
AgsI TTSAA 3 cut(s) 176, 218, 425
AluBI AGCT 5 cut(s) 78, 121, 137, 305, 539
AluI AGCT 5 cut(s) 78, 121, 137, 305, 539
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 4 cut(s) 44, 104, 204, 518
AoxI GGCC 2 cut(s) 87, 199
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 313
AspLEI GCGC 1 cut(s) 495
AsuHPI GGTGA 3 cut(s) 82, 175, 307
BanI GGYRCC 1 cut(s) 380
BbsI GAAGAC 2 cut(s) 63, 332
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 1 cut(s) 146
BccI CCATC 3 cut(s) 210, 282, 292
BclI TGATCA 1 cut(s) 27
BcoDI GTCTC 4 cut(s) 44, 104, 204, 518
BfaI CTAG 2 cut(s) 377, 453
BfuAI ACCTGC 1 cut(s) 283
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
BmcAI AGTACT 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 382, 512
BmrI ACTGGG 1 cut(s) 472
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 2 cut(s) 63, 332
BpmI CTGGAG 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 384
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 140, 240, 467
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 3 cut(s) 140, 240, 467
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 1 cut(s) 146
BshFI GGCC 2 cut(s) 89, 201
BshNI GGYRCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 402, 479
BsmAI GTCTC 4 cut(s) 44, 104, 204, 518
BsmFI GGGAC 2 cut(s) 402, 479
BsnI GGCC 2 cut(s) 89, 201
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 1 cut(s) 27
Bsp19I CCATGG 1 cut(s) 384
BspANI GGCC 2 cut(s) 89, 201
BspCNI CTCAG 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 382, 512
BspMI ACCTGC 1 cut(s) 283
BspQI GCTCTTC 1 cut(s) 426
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 3 cut(s) 140, 240, 467
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 2 cut(s) 16, 115
Bst6I CTCTTC 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 1 cut(s) 528
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 293
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 4 cut(s) 44, 104, 204, 518
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 146
BstV2I GAAGAC 2 cut(s) 63, 332
BsuRI GGCC 2 cut(s) 89, 201
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 1 cut(s) 293
BtsIMutI CAGTG 2 cut(s) 120, 233
BveI ACCTGC 1 cut(s) 283
Cac8I GCNNGC 1 cut(s) 541
CfoI GCGC 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 67
Csp6I GTAC 2 cut(s) 17, 166
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 2 cut(s) 385, 403
CviJI RGCY 8 cut(s) 78, 89, 121, 137, 201, 239, 305, 539
CviKI_1 RGCY 8 cut(s) 78, 89, 121, 137, 201, 239, 305, 539
CviQI GTAC 2 cut(s) 17, 166
DdeI CTNAG 1 cut(s) 528
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
DraI TTTAAA 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 384
Eco147I AGGCCT 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 2 cut(s) 388, 406
FaqI GGGAC 2 cut(s) 402, 479
FatI CATG 2 cut(s) 384, 402
FbaI TGATCA 1 cut(s) 27
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 1 cut(s) 300
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 2 cut(s) 377, 453
GlaI GCGC 1 cut(s) 494
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 2 cut(s) 89, 201
HapII CCGG 1 cut(s) 68
HhaI GCGC 1 cut(s) 495
Hin1II CATG 2 cut(s) 388, 406
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 3 cut(s) 172, 442, 526
HpaII CCGG 1 cut(s) 68
HphI GGTGA 3 cut(s) 82, 175, 307
Hpy188I TCNGA 4 cut(s) 46, 209, 447, 531
Hpy188III TCNNGA 1 cut(s) 453
HpyAV CCTTC 1 cut(s) 365
HpyCH4III ACNGT 2 cut(s) 16, 115
HpyCH4V TGCA 3 cut(s) 134, 146, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 528
Hsp92II CATG 2 cut(s) 388, 406
HspAI GCGC 1 cut(s) 493
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 1 cut(s) 27
LguI GCTCTTC 1 cut(s) 426
LmnI GCTCC 2 cut(s) 142, 478
LpnPI CCDG 6 cut(s) 81, 153, 221, 278, 443, 448
Lsp1109I GCAGC 1 cut(s) 146
MaeI CTAG 2 cut(s) 377, 453
MaeIII GTNAC 1 cut(s) 181
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 4 cut(s) 68, 139, 332, 413
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 1 cut(s) 313
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 4 cut(s) 76, 79, 275, 466
MseI TTAA 1 cut(s) 366
MspI CCGG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 143
NcoI CCATGG 1 cut(s) 384
NdeII GATC 1 cut(s) 27
NlaIII CATG 2 cut(s) 388, 406
NlaIV GGNNCC 2 cut(s) 382, 512
NmuCI GTSAC 1 cut(s) 181
PaqCI CACCTGC 1 cut(s) 283
PceI AGGCCT 1 cut(s) 89
PciSI GCTCTTC 1 cut(s) 426
PfeI GAWTC 2 cut(s) 172, 442
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 382, 512
RsaI GTAC 2 cut(s) 18, 167
RsaNI GTAC 2 cut(s) 17, 166
SapI GCTCTTC 1 cut(s) 426
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 1 cut(s) 27
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 1 cut(s) 520
SduI GDGCHC 1 cut(s) 421
Sse9I AATT 1 cut(s) 313
SseBI AGGCCT 1 cut(s) 89
SspMI CTAG 2 cut(s) 377, 453
StuI AGGCCT 1 cut(s) 89
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 2 cut(s) 16, 115
TasI AATT 1 cut(s) 313
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 2 cut(s) 172, 442
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 2 cut(s) 120, 240
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 181
TspRI CASTG 2 cut(s) 120, 240
XapI RAATTY 1 cut(s) 313
XbaI TCTAGA 1 cut(s) 452
XspI CTAG 2 cut(s) 377, 453
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.