pycom07g25200

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002046
Physical Location & Seq
Reverse (-)
25611921 .. 25613348
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25200.4

Sequence Viewer

Length: 810 bp
ATGAATTTTCAGAATTGGGAAATTATGTTGTTGCTTGGAATAAGTGCTGCCGTAAGTATCACATTGTTAATGGTCTACCATCTGCACATAGCAGCTCTCGCCATTGGGTCTTTAGCCATGGTTGCTATCTTCGTAAGCAGAGACTACTTTGCCATGATTTTCACCAGCAGTGGAGATATGCAACTCGCCGTTTCTCGTTTAGCTTACCTTCTTGGTGTAACGATGCTTCTTACCAGTGCTACACAAGTATTAACAGGAGTTGCTATTGGAGGTGGATGGCAAGTGATGGTGGCTTATATAAATTTTGGTAGTTATTATTTGGTTGGGCTCCCATTAGCAATCTTTCTTGGCTTCAAAGTAGATCTGGGAGCTGTGGGACTCTGGGGTGGGATGATGATTGGAAACGCTCTTCAGATCTTCTTCTTGCTGATTCTGACTTGTAGAACCAACTGGGACAGAGAGTTGAGGCGTTCCGCCTCCACTACTCAATCATCTAGTCCGACCAAAAAGAACGAAAAGGTCGCAATGAGACGACCAACAGGTTCCGAGCTGATGAGATTTTCGTCTCCATTTCTCAAAATCAATCCTTTTTCTCTAGGTTACAAGTTTATCGTTTATCCGACTTCTCCGCTTACATTGACCACAACTCGTTTACCTCGAAGACCTTATGCGGTTACCAATACAACGAGAACTATCAACCTTCCCACAGTAACAAAATCTTGTTGCATCATGTTTCCGCCTCTTCTAGCAAGGATATTGTGTAGGTTTTACTGTGGTTTTAAGCTTTTTGAGTTTTTGGTAACATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

270

Amino Acids

29.92

Weight (kDa)

9.94

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 75
AciI CCGC 4 cut(s) 474, 629, 671, 737
AcsI RAATTY 2 cut(s) 4, 301
AcuI CTGAAG 1 cut(s) 395
AgsI TTSAA 1 cut(s) 355
AluBI AGCT 5 cut(s) 95, 203, 371, 550, 784
AluI AGCT 5 cut(s) 95, 203, 371, 550, 784
Alw26I GTCTC 3 cut(s) 135, 523, 570
ApeKI GCWGC 2 cut(s) 47, 92
ApoI RAATTY 2 cut(s) 4, 301
AsuHPI GGTGA 1 cut(s) 154
BanII GRGCYC 1 cut(s) 330
BarI GAAGNNNNNNTAC 2 cut(s) 210, 242
BbsI GAAGAC 1 cut(s) 667
BbvI GCAGC 2 cut(s) 34, 104
BccI CCATC 3 cut(s) 87, 270, 280
BceAI ACGGC 2 cut(s) 35, 173
BcoDI GTCTC 3 cut(s) 135, 523, 570
BfaI CTAG 4 cut(s) 495, 596, 746, 808
BglII AGATCT 2 cut(s) 361, 414
BisI GCNGC 2 cut(s) 48, 93
BlsI GCNGC 2 cut(s) 49, 94
BmiI GGNNCC 2 cut(s) 329, 544
BmrI ACTGGG 1 cut(s) 460
BmsI GCATC 2 cut(s) 213, 735
BmuI ACTGGG 1 cut(s) 460
BpiI GAAGAC 1 cut(s) 667
BsaJI CCNNGG 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 234, 455
Bse3DI GCAATG 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 2 cut(s) 281, 396
BseMI GCAATG 1 cut(s) 531
BseNI ACTGG 2 cut(s) 234, 455
BseXI GCAGC 2 cut(s) 34, 104
BsgI GTGCAG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 390, 467
BsmAI GTCTC 3 cut(s) 135, 523, 570
BsmBI CGTCTC 2 cut(s) 523, 570
BsmFI GGGAC 2 cut(s) 390, 467
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 2 cut(s) 361, 414
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 4 cut(s) 474, 629, 671, 737
BspLI GGNNCC 2 cut(s) 329, 544
BspQI GCTCTTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 2 cut(s) 234, 455
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 361, 414
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 709, 773
Bst6I CTCTTC 2 cut(s) 414, 747
BstDSI CCRYGG 1 cut(s) 117
BstEII GGTNACC 1 cut(s) 673
BstF5I GGATG 2 cut(s) 281, 396
BstKTI GATC 2 cut(s) 364, 417
BstMAI GTCTC 3 cut(s) 135, 523, 570
BstMBI GATC 2 cut(s) 361, 414
BstMWI GCNNNNNNNGC 2 cut(s) 98, 122
BstPI GGTNACC 1 cut(s) 673
BstV1I GCAGC 2 cut(s) 34, 104
BstV2I GAAGAC 1 cut(s) 667
BstX2I RGATCY 2 cut(s) 361, 414
BstYI RGATCY 2 cut(s) 361, 414
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 2 cut(s) 281, 396
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 175, 241
CviAII CATG 3 cut(s) 118, 154, 730
CviJI RGCY 9 cut(s) 95, 116, 203, 293, 328, 351, 371, 550, 784
CviKI_1 RGCY 9 cut(s) 95, 116, 203, 293, 328, 351, 371, 550, 784
DpnI GATC 2 cut(s) 363, 416
DpnII GATC 2 cut(s) 361, 414
Eam1104I CTCTTC 2 cut(s) 414, 747
EarI CTCTTC 2 cut(s) 414, 747
EciI GGCGGA 2 cut(s) 463, 726
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 395
Eco91I GGTNACC 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 117
Esp3I CGTCTC 2 cut(s) 523, 570
FaeI CATG 3 cut(s) 121, 157, 733
FaiI YATR 9 cut(s) 26, 89, 119, 155, 179, 297, 299, 669, 731
FaqI GGGAC 2 cut(s) 390, 467
FatI CATG 3 cut(s) 117, 153, 729
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 2 cut(s) 48, 93
FokI GGATG 2 cut(s) 288, 403
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 2 cut(s) 48, 93
FspBI CTAG 4 cut(s) 495, 596, 746, 808
GluI GCNGC 2 cut(s) 48, 93
Hin1II CATG 3 cut(s) 121, 157, 733
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 2 cut(s) 378, 430
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 76, 653
Hpy188I TCNGA 6 cut(s) 12, 414, 435, 501, 547, 621
Hpy8I GTNNAC 2 cut(s) 76, 653
HpyAV CCTTC 2 cut(s) 218, 710
HpyCH4III ACNGT 2 cut(s) 709, 773
HpyCH4V TGCA 3 cut(s) 85, 181, 726
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 122
Hsp92II CATG 3 cut(s) 121, 157, 733
Kzo9I GATC 2 cut(s) 361, 414
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 333, 368
LpnPI CCDG 7 cut(s) 178, 240, 247, 350, 367, 436, 525
Lsp1109I GCAGC 2 cut(s) 34, 104
LweI GCATC 2 cut(s) 213, 735
MaeI CTAG 4 cut(s) 495, 596, 746, 808
MaeIII GTNAC 5 cut(s) 217, 599, 673, 709, 799
MalI GATC 2 cut(s) 363, 416
MboI GATC 2 cut(s) 361, 414
MboII GAAGA 6 cut(s) 121, 401, 409, 412, 672, 734
MflI RGATCY 2 cut(s) 361, 414
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 4 cut(s) 4, 13, 21, 301
MlyI GAGTC 1 cut(s) 372
MmeI TCCRAC 2 cut(s) 524, 644
MnlI CCTC 5 cut(s) 263, 459, 487, 666, 750
MseI TTAA 3 cut(s) 68, 251, 780
MwoI GCNNNNNNNGC 2 cut(s) 98, 122
NcoI CCATGG 1 cut(s) 117
NdeII GATC 2 cut(s) 361, 414
NlaIII CATG 3 cut(s) 121, 157, 733
NlaIV GGNNCC 2 cut(s) 329, 544
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PfeI GAWTC 1 cut(s) 430
PkrI GCNGC 2 cut(s) 49, 94
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PspEI GGTNACC 1 cut(s) 673
PspN4I GGNNCC 2 cut(s) 329, 544
PsuI RGATCY 2 cut(s) 361, 414
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 3 cut(s) 68, 251, 780
SatI GCNGC 2 cut(s) 48, 93
Sau3AI GATC 2 cut(s) 361, 414
SchI GAGTC 1 cut(s) 372
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 2 cut(s) 213, 735
Sse9I AATT 4 cut(s) 4, 13, 21, 301
SsiI CCGC 4 cut(s) 474, 629, 671, 737
SspMI CTAG 4 cut(s) 495, 596, 746, 808
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 709, 773
TaqI TCGA 1 cut(s) 658
TasI AATT 4 cut(s) 4, 13, 21, 301
TfiI GAWTC 1 cut(s) 430
Tru1I TTAA 3 cut(s) 68, 251, 780
Tru9I TTAA 3 cut(s) 68, 251, 780
TscAI CASTG 2 cut(s) 175, 241
TseI GCWGC 2 cut(s) 47, 92
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 175, 241
XapI RAATTY 2 cut(s) 4, 301
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 4 cut(s) 495, 596, 746, 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.