pycom01g21630

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002046
Physical Location & Seq
Reverse (-)
19579908 .. 19580355
448 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21630.2

Sequence Viewer

Length: 345 bp
ATGAATTTTTGGAATTGGGAAATTATGTGGTTGCTTGGAATAAATGCTGCTATAAGCACACGAGTCTCGAACGAAATTGGGATGGGGCGCCCGAGAGCAGCCAAATACTCCGTCTGTGTTGCAGTCTTGCAAGCTCTCATCGTCGGGGTTTTAGCCATGGTTGCTATCTTCATAAGCAGAGACTACAGTGCCATGATTTTCACCAGCAGTGGAGAGATGCAGCTCGCCGTTGCTCGTTTAGCATACCTTCTTGGTGTAACCATGCTTCTTACCAGTGCTACACAAGTCTTAACAGGTATAATCACTTCAACCAGTTTTAACCTTCCAATGAGTCTCCCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

115

Amino Acids

12.38

Weight (kDa)

9.1

Isoelectric Point (pI)

19.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 4
AcyI GRCGYC 1 cut(s) 88
AgsI TTSAA 1 cut(s) 309
AluBI AGCT 2 cut(s) 134, 223
AluI AGCT 2 cut(s) 134, 223
Alw26I GTCTC 3 cut(s) 70, 174, 338
Ama87I CYCGRG 1 cut(s) 91
ApeKI GCWGC 3 cut(s) 47, 98, 220
ApoI RAATTY 1 cut(s) 4
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AspLEI GCGC 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 91
BanI GGYRCC 1 cut(s) 87
BarI GAAGNNNNNNTAC 4 cut(s) 249, 281, 289, 321
BauI CACGAG 1 cut(s) 60
BbvI GCAGC 3 cut(s) 34, 110, 232
BccI CCATC 1 cut(s) 76
BceAI ACGGC 1 cut(s) 212
BcoDI GTCTC 3 cut(s) 70, 174, 338
BfmI CTRYAG 1 cut(s) 184
BfoI RGCGCY 1 cut(s) 91
BisI GCNGC 3 cut(s) 48, 99, 221
BlsI GCNGC 3 cut(s) 49, 100, 222
BmeT110I CYCGRG 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 89
BmsI GCATC 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 273, 312
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 273, 312
BseXI GCAGC 3 cut(s) 34, 110, 232
BshNI GGYRCC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 91
BsmAI GTCTC 3 cut(s) 70, 174, 338
BsoBI CYCGRG 1 cut(s) 91
Bsp19I CCATGG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 87
BsrI ACTGG 2 cut(s) 273, 312
BssECI CCNNGG 1 cut(s) 156
BssNI GRCGYC 1 cut(s) 88
BssSI CACGAG 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 132, 225
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstMAI GTCTC 3 cut(s) 70, 174, 338
BstMWI GCNNNNNNNGC 2 cut(s) 161, 239
BstSFI CTRYAG 1 cut(s) 184
BstV1I GCAGC 3 cut(s) 34, 110, 232
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 87
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 3 cut(s) 193, 214, 280
Cac8I GCNNGC 2 cut(s) 132, 225
CfoI GCGC 1 cut(s) 90
CviAII CATG 3 cut(s) 157, 193, 262
CviJI RGCY 4 cut(s) 101, 134, 155, 223
CviKI_1 RGCY 4 cut(s) 101, 134, 155, 223
DinI GGCGCC 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 156
EgeI GGCGCC 1 cut(s) 89
EheI GGCGCC 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 3 cut(s) 160, 196, 265
FaiI YATR 8 cut(s) 26, 53, 158, 173, 194, 244, 263, 299
FatI CATG 3 cut(s) 156, 192, 261
Fnu4HI GCNGC 3 cut(s) 48, 99, 221
FokI GGATG 1 cut(s) 94
Fsp4HI GCNGC 3 cut(s) 48, 99, 221
GlaI GCGC 1 cut(s) 89
GluI GCNGC 3 cut(s) 48, 99, 221
HaeII RGCGCY 1 cut(s) 91
HhaI GCGC 1 cut(s) 90
Hin1I GRCGYC 1 cut(s) 88
Hin1II CATG 3 cut(s) 160, 196, 265
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HinfI GANTC 2 cut(s) 63, 331
HphI GGTGA 1 cut(s) 193
Hpy188III TCNNGA 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 2 cut(s) 257, 332
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4V TGCA 3 cut(s) 122, 130, 220
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 239
Hsp92I GRCGYC 1 cut(s) 88
Hsp92II CATG 3 cut(s) 160, 196, 265
HspAI GCGC 1 cut(s) 88
KasI GGCGCC 1 cut(s) 87
LpnPI CCDG 4 cut(s) 217, 279, 286, 325
Lsp1109I GCAGC 3 cut(s) 34, 110, 232
LweI GCATC 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 256
MboII GAAGA 1 cut(s) 160
MluCI AATT 5 cut(s) 4, 13, 21, 75, 340
Mly113I GGCGCC 1 cut(s) 88
MlyI GAGTC 2 cut(s) 72, 340
MseI TTAA 2 cut(s) 290, 318
MwoI GCNNNNNNNGC 2 cut(s) 161, 239
NarI GGCGCC 1 cut(s) 88
NcoI CCATGG 1 cut(s) 156
NlaIII CATG 3 cut(s) 160, 196, 265
NlaIV GGNNCC 1 cut(s) 89
PkrI GCNGC 3 cut(s) 49, 100, 222
PleI GAGTC 2 cut(s) 71, 339
PluTI GGCGCC 1 cut(s) 91
PpsI GAGTC 2 cut(s) 71, 339
PspN4I GGNNCC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 290, 318
SatI GCNGC 3 cut(s) 48, 99, 221
SchI GAGTC 2 cut(s) 72, 340
SetI ASST 5 cut(s) 136, 225, 249, 298, 324
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 184
SfoI GGCGCC 1 cut(s) 89
Sse9I AATT 5 cut(s) 4, 13, 21, 75, 340
SspDI GGCGCC 1 cut(s) 87
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 188
TaqI TCGA 1 cut(s) 68
TasI AATT 5 cut(s) 4, 13, 21, 75, 340
Tru1I TTAA 2 cut(s) 290, 318
Tru9I TTAA 2 cut(s) 290, 318
TscAI CASTG 3 cut(s) 193, 214, 280
TseI GCWGC 3 cut(s) 47, 98, 220
TspDTI ATGAA 2 cut(s) 17, 160
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 3 cut(s) 193, 214, 280
XapI RAATTY 1 cut(s) 4
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.