MD01G1234200.v1.1

Serine threonine-protein phosphatase 4 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002676
Physical Location & Seq
Forward (+)
32197497 .. 32200591
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1234200.v1.1.491

Sequence Viewer

Length: 714 bp
ATGCCATCAAATGAAAATTCACAGCCCTCAATGAATTCTACCGGTGATGCTGATCATCAACAGGAGGCCATGCTTCCCGACGGCGTAGATAATCTATGTAAAGAGTCGAAGCTGGAGGTTTCTGAAGTTAGAGGCGTACTACAAGTTATTGCATCCACTGGGAAATTTTGGCAGGATTGGGACAAATTAAAGAGCATGCTATCCTTTCAACTGAAGCAGGTTCTATTGGAGTATCCTGAGGAAGAAATGACAAGTGAGCAGCAAATTGCTTCTTTAGGAGAAACCTATCCTGAGCTGGTGAAGAGGTTGGATGAAGCTCTTCATAATTTTACCGAAGGTCCTCCGTTTACCCTTCAGAGGCTTTGCGAGATCCTATTGGATGCAAAAAGAATCTATCCAAATCTCTCAAAGCTTGCTCTTGCATTAGAAAAGAATCTATTGGTGACATCTATGCTGACAGCCTCAACGGATCCATATCCACAATCCACGGTGCAAAATTCAGTTGAACCAGACAAAGCAATTGAAGAAGCTAAAATTCACTCTGATTCAGTAGCGCAAAATGGGGTGGAACCTATGGTAAGTGGCGATAGGGATGAAGTTATGTCAGAGGTAGTGCAAGCTGATATCGATAATGACATGACCATCGCGATGGAAGCTTTTGAAGATATAGTGGGATCATCAGACGCAAATTCAGTGCAGACGTATGATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPP4R2

Protein Analysis

238

Amino Acids

26.29

Weight (kDa)

4.22

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP4R2 PF09184 39 - 176 4.5e-15 PPP4R2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 208
AccII CGCG 1 cut(s) 647
AclWI GGATC 4 cut(s) 364, 464, 477, 682
AcsI RAATTY 6 cut(s) 16, 34, 164, 496, 534, 688
AcuI CTGAAG 3 cut(s) 144, 233, 338
AfaI GTAC 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 357
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 4 cut(s) 209, 506, 524, 662
AluBI AGCT 7 cut(s) 112, 295, 317, 412, 530, 620, 656
AluI AGCT 7 cut(s) 112, 295, 317, 412, 530, 620, 656
AlwI GGATC 4 cut(s) 364, 464, 477, 682
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 259
ApoI RAATTY 6 cut(s) 16, 34, 164, 496, 534, 688
AsiGI ACCGGT 1 cut(s) 41
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 1 cut(s) 556
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 3 cut(s) 56, 310, 454
AvaII GGWCC 1 cut(s) 338
AxyI CCTNAGG 1 cut(s) 237
BamHI GGATCC 1 cut(s) 469
BbvI GCAGC 1 cut(s) 271
BccI CCATC 3 cut(s) 13, 643, 650
BceAI ACGGC 1 cut(s) 97
BciVI GTATCC 1 cut(s) 243
BclI TGATCA 1 cut(s) 52
BfuAI ACCTGC 1 cut(s) 208
BfuI GTATCC 1 cut(s) 243
BisI GCNGC 1 cut(s) 260
BlsI GCNGC 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 2 cut(s) 471, 570
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 3 cut(s) 37, 161, 370
BmuI ACTGGG 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 291
Bsa29I ATCGAT 1 cut(s) 627
BsaBI GATNNNNATC 2 cut(s) 51, 474
BsaJI CCNNGG 1 cut(s) 486
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 163
Bse21I CCTNAGG 1 cut(s) 237
Bse8I GATNNNNATC 2 cut(s) 51, 474
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 4 cut(s) 152, 316, 385, 598
BseJI GATNNNNATC 2 cut(s) 51, 474
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMII CTCAG 2 cut(s) 228, 282
BseNI ACTGG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 271
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 1 cut(s) 68
BshTI ACCGGT 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 627
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 1 cut(s) 68
Bsp143I GATC 4 cut(s) 52, 369, 469, 674
Bsp68I TCGCGA 1 cut(s) 647
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 2 cut(s) 229, 283
BspDI ATCGAT 1 cut(s) 627
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 2 cut(s) 471, 570
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 4 cut(s) 364, 464, 477, 682
BspQI GCTCTTC 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 4 cut(s) 52, 369, 469, 674
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 2 cut(s) 296, 324
BstC8I GCNNGC 3 cut(s) 197, 414, 618
BstDEI CTNAG 3 cut(s) 237, 291, 711
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 4 cut(s) 152, 316, 385, 598
BstFNI CGCG 1 cut(s) 647
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 4 cut(s) 55, 372, 472, 677
BstMBI GATC 4 cut(s) 52, 369, 469, 674
BstMWI GCNNNNNNNGC 1 cut(s) 653
BstNSI RCATGY 1 cut(s) 199
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 1 cut(s) 271
BstX2I RGATCY 2 cut(s) 369, 469
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 2 cut(s) 369, 469
Bsu15I ATCGAT 1 cut(s) 627
Bsu36I CCTNAGG 1 cut(s) 237
BsuI GTATCC 1 cut(s) 243
BsuRI GGCC 1 cut(s) 68
BsuTUI ATCGAT 1 cut(s) 627
BtgI CCRYGG 1 cut(s) 486
BtgZI GCGATG 2 cut(s) 628, 662
BtsCI GGATG 4 cut(s) 152, 316, 385, 598
BtsIMutI CAGTG 2 cut(s) 156, 699
BtuMI TCGCGA 1 cut(s) 647
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 3 cut(s) 197, 414, 618
CfoI GCGC 1 cut(s) 556
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 338
ClaI ATCGAT 1 cut(s) 627
CseI GACGC 1 cut(s) 692
Csp6I GTAC 1 cut(s) 137
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 3 cut(s) 70, 196, 637
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 3 cut(s) 237, 291, 711
DpnI GATC 4 cut(s) 54, 371, 471, 676
DpnII GATC 4 cut(s) 52, 369, 469, 674
Eam1104I CTCTTC 2 cut(s) 296, 324
EarI CTCTTC 2 cut(s) 296, 324
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 338
Eco57I CTGAAG 3 cut(s) 144, 233, 338
Eco81I CCTNAGG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 338
EcoRI GAATTC 1 cut(s) 34
EcoRV GATATC 1 cut(s) 625
FaeI CATG 3 cut(s) 73, 199, 640
FaqI GGGAC 1 cut(s) 194
FatI CATG 3 cut(s) 69, 195, 636
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 1 cut(s) 260
FokI GGATG 4 cut(s) 139, 323, 392, 605
Fsp4HI GCNGC 1 cut(s) 260
GlaI GCGC 1 cut(s) 555
GluI GCNGC 1 cut(s) 260
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 42
HgaI GACGC 1 cut(s) 692
HhaI GCGC 1 cut(s) 556
Hin1II CATG 3 cut(s) 73, 199, 640
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HindIII AAGCTT 2 cut(s) 410, 654
HinfI GANTC 5 cut(s) 104, 390, 433, 545, 707
HpaII CCGG 1 cut(s) 42
HphI GGTGA 3 cut(s) 56, 310, 454
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 5 cut(s) 124, 357, 544, 607, 682
Hpy188III TCNNGA 4 cut(s) 77, 236, 290, 646
Hpy8I GTNNAC 1 cut(s) 348
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 329, 362
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 1 cut(s) 701
HpyCH4V TGCA 6 cut(s) 152, 383, 422, 493, 616, 697
HpyF10VI GCNNNNNNNGC 1 cut(s) 653
HpyF3I CTNAG 3 cut(s) 237, 291, 711
HpySE526I ACGT 1 cut(s) 701
Hsp92II CATG 3 cut(s) 73, 199, 640
HspAI GCGC 1 cut(s) 554
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 369, 469, 674
LguI GCTCTTC 1 cut(s) 324
Lsp1109I GCAGC 1 cut(s) 271
LweI GCATC 3 cut(s) 37, 161, 370
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 1 cut(s) 442
MalI GATC 4 cut(s) 54, 371, 471, 676
MboI GATC 4 cut(s) 52, 369, 469, 674
MboII GAAGA 5 cut(s) 254, 311, 313, 536, 674
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 2 cut(s) 369, 469
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 288
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 1 cut(s) 188
MslI CAYNNNNRTG 1 cut(s) 647
MspI CCGG 1 cut(s) 42
MunI CAATTG 1 cut(s) 519
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 1 cut(s) 653
NdeII GATC 4 cut(s) 52, 369, 469, 674
NlaIII CATG 3 cut(s) 73, 199, 640
NlaIV GGNNCC 2 cut(s) 471, 570
NmuCI GTSAC 1 cut(s) 442
NruI TCGCGA 1 cut(s) 647
NspI RCATGY 1 cut(s) 199
PaeI GCATGC 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 4 cut(s) 390, 433, 545, 707
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 1 cut(s) 261
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 338
PspN4I GGNNCC 2 cut(s) 471, 570
PspPI GGNCC 1 cut(s) 338
PspPPI RGGWCCY 1 cut(s) 338
PsuI RGATCY 2 cut(s) 369, 469
RruI TCGCGA 1 cut(s) 647
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 1 cut(s) 260
Sau3AI GATC 4 cut(s) 52, 369, 469, 674
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 1 cut(s) 113
SfaNI GCATC 3 cut(s) 37, 161, 370
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 647
SphI GCATGC 1 cut(s) 199
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 1 cut(s) 704
TaqI TCGA 2 cut(s) 107, 627
TfiI GAWTC 4 cut(s) 390, 433, 545, 707
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TscAI CASTG 2 cut(s) 163, 699
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 5 cut(s) 27, 47, 311, 327, 609
TspGWI ACGGA 2 cut(s) 333, 482
TspRI CASTG 2 cut(s) 163, 699
VpaK11BI GGWCC 1 cut(s) 338
XapI RAATTY 6 cut(s) 16, 34, 164, 496, 534, 688
XceI RCATGY 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.