Rroxscaffold_4G00277150

Serine threonine-protein phosphatase 4 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002676
Physical Location & Seq
Reverse (-)
592863 .. 596282
3420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277150.1

Sequence Viewer

Length: 726 bp
ATGGAGATACCAGCAAATGAAATTCCAGAGCCCTCGGCGAATTCAACCGGTGATGCTGAGCAGCAGCAACATGATGTTGTTGTGGTTCCTGAGGCATTAAATCACGGATCCAAGGACTCCAAGCACGAGATTGCTGATGGTGAAGTTAGAGGCATACTAGAAGTTATTGCATCCACTGGGAGATTTTGGCATGATTGGGACAAATTAAAGAGCATGCTATCCTTTCAGCTGAAGCAGGTTCTGTCAGAGTATCCTGATGCAGAAAAGACTAGTGAGCAGCAAATTACTACTTTAGGAGAAACCTACCTAGAACTGGTGAAGAGGTTGGATGAAGCTCTTCATGGTTTTACCGAAGGTCCTCCATTTACCCTTCAGAGGCTTTGTGAGATCCTATTGGATGCACGCAGCATCTACCCGAACCTCTCAAAGCTTGCTCTAGCTCTAGAAAAGAACCTATTGGTGACATCTATGCTGACAATCTCAACGGATCCGTATCCGCAATCAGTGTTGCAAAAGACAGATGAACCAGAAAAAGCAAGTGAAGAACCTAAACTTCACTCTGACTTGGTGCAAAATGGGGTGGAACCTATGGTAGGTGACAGGGATGAAGTAATGGTAGAGGTTGAGCAGGCCGATATTGACGATGACATGGCCATTGCCATGGAAGCTTTTGAAGATATAGTTGGGTCATCAGAAACAAATTCAGTGCAGACCAATAACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPP4R2

Protein Analysis

241

Amino Acids

26.75

Weight (kDa)

4.31

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP4R2 PF09184 39 - 159 8.9e-13 PPP4R2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 226
AciI CCGC 1 cut(s) 497
AclWI GGATC 5 cut(s) 102, 115, 382, 482, 495
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 3 cut(s) 21, 40, 700
AcuI CTGAAG 2 cut(s) 251, 356
AfiI CCNNNNNNNGG 3 cut(s) 313, 375, 593
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 2 cut(s) 45, 674
AhlI ACTAGT 1 cut(s) 269
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 5 cut(s) 229, 335, 430, 440, 668
AluI AGCT 5 cut(s) 229, 335, 430, 440, 668
AlwI GGATC 5 cut(s) 102, 115, 382, 482, 495
AlwNI CAGNNNCTG 1 cut(s) 241
AoxI GGCC 2 cut(s) 630, 651
ApeKI GCWGC 4 cut(s) 61, 64, 277, 405
ApoI RAATTY 3 cut(s) 21, 40, 700
AsiGI ACCGGT 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 336
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 5 cut(s) 62, 152, 328, 472, 608
AvaII GGWCC 1 cut(s) 356
AxyI CCTNAGG 1 cut(s) 90
BalI TGGCCA 1 cut(s) 653
BamHI GGATCC 2 cut(s) 107, 487
BanII GRGCYC 1 cut(s) 33
BauI CACGAG 1 cut(s) 125
BbvI GCAGC 4 cut(s) 73, 76, 289, 417
BccI CCATC 1 cut(s) 131
BciVI GTATCC 2 cut(s) 261, 504
BcuI ACTAGT 1 cut(s) 269
BfaI CTAG 5 cut(s) 158, 270, 308, 437, 443
BfuAI ACCTGC 1 cut(s) 226
BfuI GTATCC 2 cut(s) 261, 504
BisI GCNGC 4 cut(s) 62, 65, 278, 406
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 4 cut(s) 63, 66, 279, 407
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 4 cut(s) 87, 109, 489, 585
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 5 cut(s) 43, 179, 247, 388, 417
BmuI ACTGGG 1 cut(s) 186
Bpu1102I GCTNAGC 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 33, 111, 660
BsaWI WCCGGW 1 cut(s) 47
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 375, 593
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 2 cut(s) 181, 318
Bse21I CCTNAGG 1 cut(s) 90
Bse3DI GCAATG 1 cut(s) 654
Bse8I GATNNNNATC 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 33, 111, 660
BseGI GGATG 4 cut(s) 170, 334, 403, 610
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 3 cut(s) 313, 375, 593
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 2 cut(s) 48, 81
BseNI ACTGG 2 cut(s) 181, 318
BseXI GCAGC 4 cut(s) 73, 76, 289, 417
BshFI GGCC 2 cut(s) 632, 653
BshTI ACCGGT 1 cut(s) 47
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 3 cut(s) 313, 375, 593
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 2 cut(s) 632, 653
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 3 cut(s) 107, 387, 487
Bsp1720I GCTNAGC 1 cut(s) 57
Bsp19I CCATGG 1 cut(s) 660
BspACI CCGC 1 cut(s) 497
BspANI GGCC 2 cut(s) 632, 653
BspCNI CTCAG 2 cut(s) 49, 82
BspLI GGNNCC 4 cut(s) 87, 109, 489, 585
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 5 cut(s) 102, 115, 382, 482, 495
BspQI GCTCTTC 1 cut(s) 342
BsrDI GCAATG 1 cut(s) 654
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 2 cut(s) 181, 318
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 33, 111, 660
BssMI GATC 3 cut(s) 107, 387, 487
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 2 cut(s) 111, 660
Bst2BI CACGAG 1 cut(s) 125
Bst6I CTCTTC 2 cut(s) 314, 342
BstC8I GCNNGC 4 cut(s) 215, 403, 432, 630
BstDEI CTNAG 3 cut(s) 57, 90, 723
BstDSI CCRYGG 1 cut(s) 660
BstENI CCTNNNNNAGG 1 cut(s) 591
BstF5I GGATG 4 cut(s) 170, 334, 403, 610
BstKTI GATC 3 cut(s) 110, 390, 490
BstMBI GATC 3 cut(s) 107, 387, 487
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNSI RCATGY 1 cut(s) 217
BstV1I GCAGC 4 cut(s) 73, 76, 289, 417
BstX2I RGATCY 3 cut(s) 107, 387, 487
BstXI CCANNNNNNTGG 1 cut(s) 661
BstYI RGATCY 3 cut(s) 107, 387, 487
Bsu36I CCTNAGG 1 cut(s) 90
BsuI GTATCC 2 cut(s) 261, 504
BsuRI GGCC 2 cut(s) 632, 653
BtgI CCRYGG 1 cut(s) 660
BtsCI GGATG 4 cut(s) 170, 334, 403, 610
BtsIMutI CAGTG 3 cut(s) 174, 510, 711
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 4 cut(s) 215, 403, 432, 630
CaiI CAGNNNCTG 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 356
CspAI ACCGGT 1 cut(s) 47
CviAII CATG 6 cut(s) 71, 191, 214, 341, 649, 661
CviJI RGCY 9 cut(s) 31, 229, 335, 379, 430, 440, 632, 653, 668
CviKI_1 RGCY 9 cut(s) 31, 229, 335, 379, 430, 440, 632, 653, 668
DdeI CTNAG 3 cut(s) 57, 90, 723
DpnI GATC 3 cut(s) 109, 389, 489
DpnII GATC 3 cut(s) 107, 387, 487
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 2 cut(s) 314, 342
EarI CTCTTC 2 cut(s) 314, 342
Eco130I CCWWGG 2 cut(s) 111, 660
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 356
Eco57I CTGAAG 2 cut(s) 251, 356
Eco81I CCTNAGG 1 cut(s) 90
EcoNI CCTNNNNNAGG 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 111, 660
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 111, 660
FaeI CATG 6 cut(s) 74, 194, 217, 344, 652, 664
FaqI GGGAC 1 cut(s) 212
FatI CATG 6 cut(s) 70, 190, 213, 340, 648, 660
Fnu4HI GCNGC 4 cut(s) 62, 65, 278, 406
FokI GGATG 4 cut(s) 157, 341, 410, 617
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 4 cut(s) 62, 65, 278, 406
FspBI CTAG 5 cut(s) 158, 270, 308, 437, 443
GluI GCNGC 4 cut(s) 62, 65, 278, 406
HaeIII GGCC 2 cut(s) 632, 653
HapII CCGG 1 cut(s) 48
Hin1II CATG 6 cut(s) 74, 194, 217, 344, 652, 664
HindIII AAGCTT 2 cut(s) 428, 666
HinfI GANTC 1 cut(s) 116
HpaII CCGG 1 cut(s) 48
HphI GGTGA 5 cut(s) 62, 152, 328, 472, 608
Hpy188I TCNGA 4 cut(s) 247, 375, 562, 694
Hpy188III TCNNGA 4 cut(s) 26, 89, 254, 443
HpyAV CCTTC 2 cut(s) 347, 380
HpyCH4V TGCA 6 cut(s) 170, 260, 401, 511, 571, 709
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 3 cut(s) 57, 90, 723
Hsp92II CATG 6 cut(s) 74, 194, 217, 344, 652, 664
Kzo9I GATC 3 cut(s) 107, 387, 487
LguI GCTCTTC 1 cut(s) 342
Lsp1109I GCAGC 4 cut(s) 73, 76, 289, 417
LweI GCATC 5 cut(s) 43, 179, 247, 388, 417
MaeI CTAG 5 cut(s) 158, 270, 308, 437, 443
MaeIII GTNAC 2 cut(s) 460, 596
MalI GATC 3 cut(s) 109, 389, 489
MboI GATC 3 cut(s) 107, 387, 487
MboII GAAGA 4 cut(s) 329, 331, 554, 686
MflI RGATCY 3 cut(s) 107, 387, 487
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 5 cut(s) 21, 40, 203, 282, 700
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 8 cut(s) 43, 85, 143, 315, 369, 369, 431, 613
Mox20I TGGCCA 1 cut(s) 653
MroXI GAANNNNTTC 1 cut(s) 336
MscI TGGCCA 1 cut(s) 653
MseI TTAA 2 cut(s) 98, 206
MslI CAYNNNNRTG 1 cut(s) 659
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 1 cut(s) 229
MspI CCGG 1 cut(s) 48
MwoI GCNNNNNNNGC 1 cut(s) 665
NcoI CCATGG 1 cut(s) 660
NdeII GATC 3 cut(s) 107, 387, 487
NlaIII CATG 6 cut(s) 74, 194, 217, 344, 652, 664
NlaIV GGNNCC 4 cut(s) 87, 109, 489, 585
NmeAIII GCCGAG 1 cut(s) 14
NmuCI GTSAC 2 cut(s) 460, 596
NspI RCATGY 1 cut(s) 217
PaeI GCATGC 1 cut(s) 217
PciSI GCTCTTC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 336
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 4 cut(s) 63, 66, 279, 407
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PpuMI RGGWCCY 1 cut(s) 356
Psp5II RGGWCCY 1 cut(s) 356
PspN4I GGNNCC 4 cut(s) 87, 109, 489, 585
PspPI GGNCC 1 cut(s) 356
PspPPI RGGWCCY 1 cut(s) 356
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 3 cut(s) 107, 387, 487
PvuII CAGCTG 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 659
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 98, 206
SatI GCNGC 4 cut(s) 62, 65, 278, 406
Sau3AI GATC 3 cut(s) 107, 387, 487
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 1 cut(s) 33
SfaNI GCATC 5 cut(s) 43, 179, 247, 388, 417
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 659
SpeI ACTAGT 1 cut(s) 269
SphI GCATGC 1 cut(s) 217
Sse9I AATT 5 cut(s) 21, 40, 203, 282, 700
SsiI CCGC 1 cut(s) 497
SspMI CTAG 5 cut(s) 158, 270, 308, 437, 443
StyI CCWWGG 2 cut(s) 111, 660
TasI AATT 5 cut(s) 21, 40, 203, 282, 700
Tru1I TTAA 2 cut(s) 98, 206
Tru9I TTAA 2 cut(s) 98, 206
TscAI CASTG 3 cut(s) 181, 510, 711
TseFI GTSAC 2 cut(s) 460, 596
TseI GCWGC 4 cut(s) 61, 64, 277, 405
Tsp45I GTSAC 2 cut(s) 460, 596
TspDTI ATGAA 5 cut(s) 33, 329, 345, 537, 621
TspGWI ACGGA 3 cut(s) 120, 480, 500
TspRI CASTG 3 cut(s) 181, 510, 711
VpaK11BI GGWCC 1 cut(s) 356
XagI CCTNNNNNAGG 1 cut(s) 591
XapI RAATTY 3 cut(s) 21, 40, 700
XbaI TCTAGA 1 cut(s) 442
XceI RCATGY 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 5 cut(s) 158, 270, 308, 437, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.