MD07G1306400.v1.1

Serine threonine-protein phosphatase 4 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002676
Physical Location & Seq
Forward (+)
36297132 .. 36300710
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1306400.v1.1.491

Sequence Viewer

Length: 717 bp
ATGCCATCAAATGAAAATTCACAGCCCTCAATGAATTCTACCAGCAATGCTGATCATCAACAGGAGGCCATGCTTCCCGATGGCGTAGATAATCTATGTAAAGAGTCGAAGCTGGAGTTTTCTGAAGCAGAAGTTAGAGACGTGCTAGAAGTTATTGCATCCACTGGGAAATTTTGGCATGATTGGGACAAATTAAAGAGCATGCTATCCTTTCAGTTGAAGCAGGTTCTATTGGAGTATCCTGAGGAAAAAATGGCAAGTGAGCAGCAAATTACTTCTTTAGGAGAAACCTATCCTGAGCTGGTGAAGAGGTTGGATGAAGCTCTTCATAGTTTTACCGAAGGTCCTCTGTTTACCCTTCAGAGGCTTTGCGAGATCCTTTTGGATGCAAAAAGAATCTATCCAAATCTCTCAAAGCTTGTTCTTGCATTAGAAAAGAATCTATTGGTGACATCTATGCTGACAGTCTCAACGGATCCATATCCACAAGCCACGGTGCAAAATTCAGTTGAACCAGACAATGCAATTGAAGAACCTAAAATTCACTCTGATTCAGTGCAAAATGGGGTGGAACCTGTGGTAGGCGGCGATAGCGATGAAGTTATGGCAGAGGTAGAGCAAGCTGATATCGACGATGACATGACCATTGCCGTGGAAGCTTTTGAAGATATAGTTGGATCATCAGACGCAAATTCAGTGCAGACCAATAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPP4R2

Protein Analysis

239

Amino Acids

26.41

Weight (kDa)

4.19

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP4R2 PF09184 42 - 160 9.6e-15 PPP4R2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 214
AciI CCGC 1 cut(s) 585
AclWI GGATC 4 cut(s) 370, 470, 483, 685
AcsI RAATTY 6 cut(s) 16, 34, 170, 502, 540, 691
AcuI CTGAAG 2 cut(s) 144, 344
AfiI CCNNNNNNNGG 2 cut(s) 363, 581
AgsI TTSAA 4 cut(s) 220, 512, 530, 665
AjiI CACGTC 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 6 cut(s) 112, 301, 323, 418, 623, 659
AluI AGCT 6 cut(s) 112, 301, 323, 418, 623, 659
Alw26I GTCTC 2 cut(s) 132, 472
AlwI GGATC 4 cut(s) 370, 470, 483, 685
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 265
ApoI RAATTY 6 cut(s) 16, 34, 170, 502, 540, 691
Asp700I GAANNNNTTC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 316, 460
AvaII GGWCC 1 cut(s) 344
AxyI CCTNAGG 1 cut(s) 243
BamHI GGATCC 1 cut(s) 475
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 13, 74
BceAI ACGGC 1 cut(s) 635
BciVI GTATCC 1 cut(s) 249
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 132, 472
BfaI CTAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 214
BfuI GTATCC 1 cut(s) 249
BisI GCNGC 2 cut(s) 266, 586
BlsI GCNGC 2 cut(s) 267, 587
Bme18I GGWCC 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 2 cut(s) 477, 573
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 167, 376
BmuI ACTGGG 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 2 cut(s) 492, 651
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 581
Bse1I ACTGG 1 cut(s) 169
Bse21I CCTNAGG 1 cut(s) 243
Bse3DI GCAATG 2 cut(s) 52, 645
Bse8I GATNNNNATC 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 492, 651
BseGI GGATG 3 cut(s) 158, 322, 391
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 2 cut(s) 363, 581
BseMI GCAATG 2 cut(s) 52, 645
BseMII CTCAG 2 cut(s) 234, 288
BseNI ACTGG 1 cut(s) 169
BseXI GCAGC 1 cut(s) 277
BshFI GGCC 1 cut(s) 68
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 363, 581
BsmAI GTCTC 2 cut(s) 132, 472
BsmBI CGTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 1 cut(s) 68
Bsp143I GATC 4 cut(s) 52, 375, 475, 677
BspACI CCGC 1 cut(s) 585
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 2 cut(s) 235, 289
BspLI GGNNCC 2 cut(s) 477, 573
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 4 cut(s) 370, 470, 483, 685
BspQI GCTCTTC 1 cut(s) 330
BsrDI GCAATG 2 cut(s) 52, 645
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 492, 651
BssMI GATC 4 cut(s) 52, 375, 475, 677
Bst4CI ACNGT 2 cut(s) 466, 496
Bst6I CTCTTC 2 cut(s) 302, 330
BstC8I GCNNGC 2 cut(s) 203, 621
BstDEI CTNAG 3 cut(s) 243, 297, 714
BstDSI CCRYGG 2 cut(s) 492, 651
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 3 cut(s) 158, 322, 391
BstKTI GATC 4 cut(s) 55, 378, 478, 680
BstMAI GTCTC 2 cut(s) 132, 472
BstMBI GATC 4 cut(s) 52, 375, 475, 677
BstMWI GCNNNNNNNGC 2 cut(s) 591, 656
BstNSI RCATGY 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 2 cut(s) 375, 475
BstXI CCANNNNNNTGG 1 cut(s) 652
BstYI RGATCY 2 cut(s) 375, 475
Bsu36I CCTNAGG 1 cut(s) 243
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 2 cut(s) 492, 651
BtgZI GCGATG 1 cut(s) 609
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 3 cut(s) 158, 322, 391
BtsIMutI CAGTG 3 cut(s) 162, 561, 702
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 203, 621
Cfr13I GGNCC 1 cut(s) 344
CseI GACGC 1 cut(s) 695
CviAII CATG 4 cut(s) 70, 179, 202, 640
DdeI CTNAG 3 cut(s) 243, 297, 714
DpnI GATC 4 cut(s) 54, 377, 477, 679
DpnII GATC 4 cut(s) 52, 375, 475, 677
Eam1104I CTCTTC 2 cut(s) 302, 330
EarI CTCTTC 2 cut(s) 302, 330
Eco32I GATATC 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 2 cut(s) 144, 344
Eco81I CCTNAGG 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 344
EcoRI GAATTC 1 cut(s) 34
EcoRV GATATC 1 cut(s) 628
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 4 cut(s) 73, 182, 205, 643
FaqI GGGAC 1 cut(s) 200
FatI CATG 4 cut(s) 69, 178, 201, 639
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 266, 586
FokI GGATG 3 cut(s) 145, 329, 398
Fsp4HI GCNGC 2 cut(s) 266, 586
FspBI CTAG 1 cut(s) 146
GluI GCNGC 2 cut(s) 266, 586
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 1 cut(s) 68
HgaI GACGC 1 cut(s) 695
Hin1II CATG 4 cut(s) 73, 182, 205, 643
HindIII AAGCTT 2 cut(s) 416, 657
HinfI GANTC 4 cut(s) 104, 396, 439, 551
HphI GGTGA 2 cut(s) 316, 460
Hpy166II GTNNAC 1 cut(s) 354
Hpy188I TCNGA 4 cut(s) 124, 363, 550, 685
Hpy188III TCNNGA 3 cut(s) 77, 242, 296
Hpy8I GTNNAC 1 cut(s) 354
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 2 cut(s) 335, 368
HpyCH4III ACNGT 2 cut(s) 466, 496
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 7 cut(s) 158, 389, 428, 499, 524, 559, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 591, 656
HpyF3I CTNAG 3 cut(s) 243, 297, 714
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 4 cut(s) 73, 182, 205, 643
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 375, 475, 677
LguI GCTCTTC 1 cut(s) 330
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 2 cut(s) 167, 376
MaeI CTAG 1 cut(s) 146
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 1 cut(s) 448
MalI GATC 4 cut(s) 54, 377, 477, 679
MboI GATC 4 cut(s) 52, 375, 475, 677
MboII GAAGA 4 cut(s) 317, 319, 542, 677
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 2 cut(s) 375, 475
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 294, 655
MnlI CCTC 7 cut(s) 37, 58, 238, 303, 357, 357, 604
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 650
MunI CAATTG 1 cut(s) 525
MwoI GCNNNNNNNGC 2 cut(s) 591, 656
NdeII GATC 4 cut(s) 52, 375, 475, 677
NlaIII CATG 4 cut(s) 73, 182, 205, 643
NlaIV GGNNCC 2 cut(s) 477, 573
NmuCI GTSAC 1 cut(s) 448
NspI RCATGY 1 cut(s) 205
PaeI GCATGC 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 3 cut(s) 396, 439, 551
PkrI GCNGC 2 cut(s) 267, 587
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 344
Psp5II RGGWCCY 1 cut(s) 344
PspN4I GGNNCC 2 cut(s) 477, 573
PspPI GGNCC 1 cut(s) 344
PspPPI RGGWCCY 1 cut(s) 344
PsuI RGATCY 2 cut(s) 375, 475
RseI CAYNNNNRTG 1 cut(s) 650
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 194
SatI GCNGC 2 cut(s) 266, 586
Sau3AI GATC 4 cut(s) 52, 375, 475, 677
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 1 cut(s) 113
SfaNI GCATC 2 cut(s) 167, 376
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 650
SphI GCATGC 1 cut(s) 205
SsiI CCGC 1 cut(s) 585
SspMI CTAG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 466, 496
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 107, 630
TauI GCSGC 1 cut(s) 588
TfiI GAWTC 3 cut(s) 396, 439, 551
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TscAI CASTG 3 cut(s) 169, 561, 702
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 5 cut(s) 27, 47, 317, 333, 612
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 3 cut(s) 169, 561, 702
VpaK11BI GGWCC 1 cut(s) 344
XagI CCTNNNNNAGG 1 cut(s) 579
XapI RAATTY 6 cut(s) 16, 34, 170, 502, 540, 691
XceI RCATGY 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.