Rh1DG459500

Serine threonine-protein phosphatase 4 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002676
Physical Location & Seq
Forward (+)
66371744 .. 66375284
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG459500.1

Sequence Viewer

Length: 732 bp
ATGGAGATACCATCAAATGAAATTCCAGAGCCCTCGGCGAATTCAACCGGTGATGATGCTGAGCAGCAGCAACATCATGTTGTTGTGGTGGTTCCTGACGCATTAAATCACGGATCCAAGGACTCCAAGCACGAGATTGCTGATGGTGAAGTTAGAGGCATACTAGAAGTTATTGCATCCACTGGGAGATTTTGGCATGATTGGGACAAATTAAAGAGCATGCTATCCTTTCAGCTGAAGCAGGTTCTGTCAGAGTATCCTGATGCAGAAAAGACTAGTGAGCAGCAAATTACTACTTTAGGAGAAACCTACCTAGAACTGGTGAAGAGGTTGGATGAAGCTCTTCATGGTTTTACTGAAGGTCCTCCATTTACCCTTCAGAGGCTTTGTGAGATCCTATTGGATGCACGCAGCATCTACCCGAACCTCTCAAAGCTTGCTCTAGCTCTAGAAAAGAACCTATTGGTGACATCTGTGCTGACAATCTCAACGGATCCGTATCCGCAATCAGTGTTGCAAAAGACAGATGAACCAGAAAAAGCAAGTGAAGAACCTAAACTTCACTCTGACTTAGTGCAAAATGGGGTGGAACCTATGGTAGGTGACAGGGATGAAGTAATGGTAGAGGTTGAGCAGGCCGATATCGACGATGACATGGCCATTGCCATGGAAGCTTTTGAAGATATAGTTGGGTCATCAGAAACAAATTCAGTGCAGACCAATAACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPP4R2

Protein Analysis

243

Amino Acids

26.95

Weight (kDa)

4.34

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP4R2 PF09184 41 - 161 1.6e-12 PPP4R2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
AciI CCGC 1 cut(s) 503
AclWI GGATC 5 cut(s) 108, 121, 388, 488, 501
AcoI YGGCCR 1 cut(s) 657
AcsI RAATTY 3 cut(s) 21, 40, 706
AcuI CTGAAG 3 cut(s) 257, 362, 378
AfiI CCNNNNNNNGG 3 cut(s) 319, 381, 599
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 2 cut(s) 45, 680
AhlI ACTAGT 1 cut(s) 275
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
AluBI AGCT 5 cut(s) 235, 341, 436, 446, 674
AluI AGCT 5 cut(s) 235, 341, 436, 446, 674
AlwI GGATC 5 cut(s) 108, 121, 388, 488, 501
AlwNI CAGNNNCTG 1 cut(s) 247
AoxI GGCC 2 cut(s) 636, 657
ApeKI GCWGC 4 cut(s) 64, 67, 283, 411
ApoI RAATTY 3 cut(s) 21, 40, 706
AsiGI ACCGGT 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 362
AsuHPI GGTGA 5 cut(s) 62, 158, 334, 478, 614
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 659
BamHI GGATCC 2 cut(s) 113, 493
BanII GRGCYC 1 cut(s) 33
BauI CACGAG 1 cut(s) 131
BbvI GCAGC 4 cut(s) 76, 79, 295, 423
BccI CCATC 2 cut(s) 19, 137
BciVI GTATCC 2 cut(s) 267, 510
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 5 cut(s) 164, 276, 314, 443, 449
BfuAI ACCTGC 1 cut(s) 232
BfuI GTATCC 2 cut(s) 267, 510
BisI GCNGC 4 cut(s) 65, 68, 284, 412
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 4 cut(s) 66, 69, 285, 413
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 362
BmiI GGNNCC 4 cut(s) 93, 115, 495, 591
BmrI ACTGGG 1 cut(s) 192
BmsI GCATC 5 cut(s) 46, 185, 253, 394, 423
BmuI ACTGGG 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 498
BsaJI CCNNGG 3 cut(s) 33, 117, 666
BsaWI WCCGGW 1 cut(s) 47
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 381, 599
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 2 cut(s) 187, 324
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 498
BseDI CCNNGG 3 cut(s) 33, 117, 666
BseGI GGATG 4 cut(s) 176, 340, 409, 616
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 3 cut(s) 319, 381, 599
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 2 cut(s) 187, 324
BseXI GCAGC 4 cut(s) 76, 79, 295, 423
BshFI GGCC 2 cut(s) 638, 659
BshTI ACCGGT 1 cut(s) 47
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 3 cut(s) 319, 381, 599
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 2 cut(s) 638, 659
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 3 cut(s) 113, 393, 493
Bsp1720I GCTNAGC 1 cut(s) 60
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 1 cut(s) 503
BspANI GGCC 2 cut(s) 638, 659
BspCNI CTCAG 1 cut(s) 52
BspLI GGNNCC 4 cut(s) 93, 115, 495, 591
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 5 cut(s) 108, 121, 388, 488, 501
BspQI GCTCTTC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 2 cut(s) 187, 324
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 33, 117, 666
BssMI GATC 3 cut(s) 113, 393, 493
BssSI CACGAG 1 cut(s) 131
BssT1I CCWWGG 2 cut(s) 117, 666
Bst2BI CACGAG 1 cut(s) 131
Bst6I CTCTTC 2 cut(s) 320, 348
BstC8I GCNNGC 4 cut(s) 221, 409, 438, 636
BstDEI CTNAG 3 cut(s) 60, 571, 729
BstDSI CCRYGG 1 cut(s) 666
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 4 cut(s) 176, 340, 409, 616
BstKTI GATC 3 cut(s) 116, 396, 496
BstMBI GATC 3 cut(s) 113, 393, 493
BstMWI GCNNNNNNNGC 1 cut(s) 671
BstNSI RCATGY 1 cut(s) 223
BstV1I GCAGC 4 cut(s) 76, 79, 295, 423
BstX2I RGATCY 3 cut(s) 113, 393, 493
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 3 cut(s) 113, 393, 493
BsuI GTATCC 2 cut(s) 267, 510
BsuRI GGCC 2 cut(s) 638, 659
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 4 cut(s) 176, 340, 409, 616
BtsIMutI CAGTG 3 cut(s) 180, 516, 717
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 4 cut(s) 221, 409, 438, 636
CaiI CAGNNNCTG 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 362
CseI GACGC 1 cut(s) 107
CspAI ACCGGT 1 cut(s) 47
CviAII CATG 6 cut(s) 77, 197, 220, 347, 655, 667
CviJI RGCY 9 cut(s) 31, 235, 341, 385, 436, 446, 638, 659, 674
CviKI_1 RGCY 9 cut(s) 31, 235, 341, 385, 436, 446, 638, 659, 674
DdeI CTNAG 3 cut(s) 60, 571, 729
DpnI GATC 3 cut(s) 115, 395, 495
DpnII GATC 3 cut(s) 113, 393, 493
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 2 cut(s) 320, 348
EarI CTCTTC 2 cut(s) 320, 348
Eco130I CCWWGG 2 cut(s) 117, 666
Eco24I GRGCYC 1 cut(s) 33
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 362
Eco57I CTGAAG 3 cut(s) 257, 362, 378
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 40
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 117, 666
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 117, 666
FaeI CATG 6 cut(s) 80, 200, 223, 350, 658, 670
FaiI YATR 9 cut(s) 78, 161, 198, 221, 348, 596, 656, 668, 686
FaqI GGGAC 1 cut(s) 218
FatI CATG 6 cut(s) 76, 196, 219, 346, 654, 666
Fnu4HI GCNGC 4 cut(s) 65, 68, 284, 412
FokI GGATG 4 cut(s) 163, 347, 416, 623
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 4 cut(s) 65, 68, 284, 412
FspBI CTAG 5 cut(s) 164, 276, 314, 443, 449
GluI GCNGC 4 cut(s) 65, 68, 284, 412
HaeIII GGCC 2 cut(s) 638, 659
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 107
Hin1II CATG 6 cut(s) 80, 200, 223, 350, 658, 670
HindIII AAGCTT 2 cut(s) 434, 672
HinfI GANTC 1 cut(s) 122
HpaII CCGG 1 cut(s) 48
HphI GGTGA 5 cut(s) 62, 158, 334, 478, 614
Hpy188I TCNGA 4 cut(s) 253, 381, 568, 700
Hpy188III TCNNGA 4 cut(s) 26, 95, 260, 449
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 2 cut(s) 353, 386
HpyCH4V TGCA 6 cut(s) 176, 266, 407, 517, 577, 715
HpyF10VI GCNNNNNNNGC 1 cut(s) 671
HpyF3I CTNAG 3 cut(s) 60, 571, 729
Hsp92II CATG 6 cut(s) 80, 200, 223, 350, 658, 670
Kzo9I GATC 3 cut(s) 113, 393, 493
LguI GCTCTTC 1 cut(s) 348
Lsp1109I GCAGC 4 cut(s) 76, 79, 295, 423
LweI GCATC 5 cut(s) 46, 185, 253, 394, 423
MaeI CTAG 5 cut(s) 164, 276, 314, 443, 449
MaeIII GTNAC 2 cut(s) 466, 602
MalI GATC 3 cut(s) 115, 395, 495
MboI GATC 3 cut(s) 113, 393, 493
MboII GAAGA 4 cut(s) 335, 337, 560, 692
MflI RGATCY 3 cut(s) 113, 393, 493
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 659
MluCI AATT 5 cut(s) 21, 40, 209, 288, 706
MluNI TGGCCA 1 cut(s) 659
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 7 cut(s) 43, 149, 321, 375, 375, 437, 619
Mox20I TGGCCA 1 cut(s) 659
MroXI GAANNNNTTC 1 cut(s) 342
MscI TGGCCA 1 cut(s) 659
MseI TTAA 2 cut(s) 104, 212
MslI CAYNNNNRTG 1 cut(s) 665
Msp20I TGGCCA 1 cut(s) 659
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 1 cut(s) 48
MwoI GCNNNNNNNGC 1 cut(s) 671
NcoI CCATGG 1 cut(s) 666
NdeII GATC 3 cut(s) 113, 393, 493
NlaIII CATG 6 cut(s) 80, 200, 223, 350, 658, 670
NlaIV GGNNCC 4 cut(s) 93, 115, 495, 591
NmeAIII GCCGAG 1 cut(s) 14
NmuCI GTSAC 2 cut(s) 466, 602
NspI RCATGY 1 cut(s) 223
PaeI GCATGC 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 342
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 4 cut(s) 66, 69, 285, 413
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PpuMI RGGWCCY 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 362
PspN4I GGNNCC 4 cut(s) 93, 115, 495, 591
PspPI GGNCC 1 cut(s) 362
PspPPI RGGWCCY 1 cut(s) 362
PstNI CAGNNNCTG 1 cut(s) 247
PsuI RGATCY 3 cut(s) 113, 393, 493
PvuII CAGCTG 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 665
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 2 cut(s) 104, 212
SatI GCNGC 4 cut(s) 65, 68, 284, 412
Sau3AI GATC 3 cut(s) 113, 393, 493
Sau96I GGNCC 1 cut(s) 362
SchI GAGTC 1 cut(s) 116
SduI GDGCHC 1 cut(s) 33
SfaNI GCATC 5 cut(s) 46, 185, 253, 394, 423
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 665
SpeI ACTAGT 1 cut(s) 275
SphI GCATGC 1 cut(s) 223
Sse9I AATT 5 cut(s) 21, 40, 209, 288, 706
SsiI CCGC 1 cut(s) 503
SspMI CTAG 5 cut(s) 164, 276, 314, 443, 449
StyI CCWWGG 2 cut(s) 117, 666
TaqI TCGA 1 cut(s) 645
TasI AATT 5 cut(s) 21, 40, 209, 288, 706
Tru1I TTAA 2 cut(s) 104, 212
Tru9I TTAA 2 cut(s) 104, 212
TscAI CASTG 3 cut(s) 187, 516, 717
TseFI GTSAC 2 cut(s) 466, 602
TseI GCWGC 4 cut(s) 64, 67, 283, 411
Tsp45I GTSAC 2 cut(s) 466, 602
TspDTI ATGAA 5 cut(s) 33, 335, 351, 543, 627
TspGWI ACGGA 3 cut(s) 126, 486, 506
TspRI CASTG 3 cut(s) 187, 516, 717
VpaK11BI GGWCC 1 cut(s) 362
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 3 cut(s) 21, 40, 706
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 5 cut(s) 164, 276, 314, 443, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.