MD02G1160900.v1.1

Cell division cycle-associated protein 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001920
Physical Location & Seq
Reverse (-)
13492593 .. 13495986
3394 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1160900.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGCCAACTCTGGGAATGAAGACTGTTGCCCTAGAATCCTCAGCAAACCCTAACCCTCAAAGCAATAATGAGCACCCCCAAACCCCCAAGTCTCTGTACGAGCAGTCCAGAGAAGATCGAATCAGAGAAAACCGCGAAAGAATGCAAAAGCTCGGCATCGTTGATATCTCTCTTCAGCTCAAATCTAACTTTCAATCGAATAGATCAACGAAGAGCTATTCCGGCCGAAGTACGATGCCGTCTGGACCCACTTCCATTCGGTCACGAGGACCCACTCGTCGTTCGTCTAGAATGTGGATTGGATTGAAGAATGCGACACCAGTGAGCTATGCAGAGGTGCATGTGTCGAAGAAGGATGAGGCTTCGTACATGAAAGGTATTATGCTGGAGGAAGGTGGTAGGCCGGAGATTTACACAGAGGAGCATGAGAAGCTGTTGGGCAACACTGATAAGACCTGGACTCTATTTGTGGATGGTTATGGAAAAGATGGAAAGCGGTTTTATGACCCGGATAGAGGAAAGACTTGCCATCAATGCAGGCAGAAAACTCTGGGCCATCATACTCAATGCAACCAATGCAACAGGTTCCAAGGGCAGTTTTGTGGCGATTGTCTCTACATGCGATATGGGGAACATGTAATTGAAGCCAATCAGAATCCAGATTGGATTTGCCCTGTCTGCCGTGGAATTTGCAACTGTAGTTTCTGTCGGACAAAAAAAGGATGGCCTCCTACTGGCATCCTCTACAAGAAGATATCACAACTAGGTTTCAAATCAGTTGCACACTATCTCATTCACACCCAACGAGGACAAACGGATTCAGAAGAAAATCTGGAAACCACCAATCAAGTTTCTGCAAAGAGGTCATTGCAATTCCCAGATGTAGATGCATCATGTGAAGAAATTGTCGAGGACAACAATGATATTGGAGCAATAAAACTTCCAACTTGGCAAGAAAGAGATGATGAGTTTAAGAGTGAGAAGGAGAATGATATCGAGAATAGCACAAATCTAGATATTAACAAACAAACTTCTGCAAAGAGGGCATTGTCCTTTTCAGATGTGGTACCACAACCTGGAAGTTTTGGATCACCTGAGGTTGACCACAAGGTTGGTGACCATCTTGGATTGTCAAAGCTTCAATCTGAGACTGAGAGTAGCAGAGATGATCGTCAAGGTGAAAAAGAGAATACAATGCCTTTGATGGATATGAAACTTGGTAATGGCAATACATTGGAAACTAGCTCAAAGTCTGAAACGAAGCCTGCTCTGGATATTGAGCCAAGCACTGATAGTATTGGTGGAAGACTAAGGCAGAGACGCAGGAAAGGAAACAAGCTTAAGGATGATGACTTACAAGAGGAAAAAATGGAAATTCCAGATAGCAGTCCTGCATTGGGCACAGAGCTCAAGAAGAAACGTGTTCTTGCTGAACCAAGTCCAGACAGCATTGGTGGAAGATTGAGGCAGAGGCGCAAGGGCAACGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-4CXXC_R1

Protein Analysis

497

Amino Acids

55.98

Weight (kDa)

8.32

Isoelectric Point (pI)

54.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 168 - 264 3.9e-41 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 276
Acc65I GGTACC 1 cut(s) 1066
AccB1I GGYRCC 1 cut(s) 1066
AccB7I CCANNNNNTGG 1 cut(s) 1076
AccII CGCG 1 cut(s) 135
AciI CCGC 2 cut(s) 133, 496
AclWI GGATC 1 cut(s) 1096
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 2 cut(s) 687, 1374
AcuI CTGAAG 1 cut(s) 158
AfaI GTAC 4 cut(s) 98, 232, 368, 1068
AfiI CCNNNNNNNGG 5 cut(s) 11, 515, 734, 1076, 1397
AflII CTTAAG 1 cut(s) 1340
AflIII ACRYGT 2 cut(s) 634, 1420
AgsI TTSAA 5 cut(s) 194, 307, 644, 772, 1142
AjnI CCWGG 2 cut(s) 455, 1075
AluBI AGCT 9 cut(s) 151, 178, 216, 327, 433, 1138, 1245, 1339, 1408
AluI AGCT 9 cut(s) 151, 178, 216, 327, 433, 1138, 1245, 1339, 1408
Alw21I GWGCWC 2 cut(s) 75, 1410
Alw26I GTCTC 4 cut(s) 96, 617, 1142, 1312
AlwI GGATC 1 cut(s) 1096
AoxI GGCC 4 cut(s) 223, 401, 553, 725
ApoI RAATTY 2 cut(s) 687, 1374
Asp718I GGTACC 1 cut(s) 1066
AspLEI GCGC 1 cut(s) 1476
AspS9I GGNCC 3 cut(s) 245, 269, 553
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 3 cut(s) 1083, 1127, 1190
AvaII GGWCC 2 cut(s) 245, 269
AxyI CCTNAGG 1 cut(s) 1095
BaeGI GKGCMC 1 cut(s) 1403
BanI GGYRCC 1 cut(s) 1066
BanII GRGCYC 1 cut(s) 1410
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 2 cut(s) 26, 1312
Bbv12I GWGCWC 2 cut(s) 75, 1410
BbvCI CCTCAGC 1 cut(s) 40
BccI CCATC 7 cut(s) 467, 482, 537, 564, 717, 1128, 1198
BceAI ACGGC 2 cut(s) 223, 666
BciT130I CCWGG 2 cut(s) 457, 1077
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 4 cut(s) 96, 617, 1142, 1312
BfaI CTAG 5 cut(s) 32, 288, 764, 1013, 1242
BfmI CTRYAG 1 cut(s) 697
BfrI CTTAAG 1 cut(s) 1340
Bme1390I CCNGG 3 cut(s) 457, 509, 1077
Bme18I GGWCC 2 cut(s) 245, 269
BmgT120I GGNCC 3 cut(s) 245, 269, 553
BmiI GGNNCC 4 cut(s) 247, 271, 587, 1068
BmrFI CCNGG 3 cut(s) 457, 509, 1077
BmsI GCATC 5 cut(s) 165, 225, 747, 877, 899
BpiI GAAGAC 2 cut(s) 26, 1312
BpmI CTGGAG 1 cut(s) 407
Bpu10I CCTNAGC 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 1 cut(s) 509
BsaJI CCNNGG 2 cut(s) 589, 682
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 515, 734, 1076, 1397
Bse1I ACTGG 2 cut(s) 320, 739
Bse21I CCTNAGG 1 cut(s) 1095
Bse3DI GCAATG 1 cut(s) 866
BseBI CCWGG 2 cut(s) 457, 1077
BseDI CCNNGG 2 cut(s) 589, 682
BseGI GGATG 5 cut(s) 361, 478, 728, 738, 1351
BseLI CCNNNNNNNGG 5 cut(s) 11, 515, 734, 1076, 1397
BseMI GCAATG 1 cut(s) 866
BseMII CTCAG 4 cut(s) 54, 1086, 1137, 1143
BseNI ACTGG 2 cut(s) 320, 739
BseRI GAGGAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 1403
BseX3I CGGCCG 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 4 cut(s) 225, 403, 555, 727
BshNI GGYRCC 1 cut(s) 1066
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 75, 1410
BsiSI CCGG 3 cut(s) 222, 404, 509
BslI CCNNNNNNNGG 5 cut(s) 11, 515, 734, 1076, 1397
BsmAI GTCTC 4 cut(s) 96, 617, 1142, 1312
BsmBI CGTCTC 1 cut(s) 1312
BsmI GAATGC 2 cut(s) 147, 316
BsnI GGCC 4 cut(s) 225, 403, 555, 727
Bsp1286I GDGCHC 3 cut(s) 75, 1403, 1410
Bsp143I GATC 4 cut(s) 115, 203, 1088, 1168
BspACI CCGC 2 cut(s) 133, 496
BspANI GGCC 4 cut(s) 225, 403, 555, 727
BspCNI CTCAG 4 cut(s) 53, 1087, 1138, 1144
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 4 cut(s) 247, 271, 587, 1068
BspPI GGATC 1 cut(s) 1096
BspQI GCTCTTC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 1066
BspTI CTTAAG 1 cut(s) 1340
BsrDI GCAATG 1 cut(s) 866
BsrI ACTGG 2 cut(s) 320, 739
BssECI CCNNGG 2 cut(s) 589, 682
BssMI GATC 4 cut(s) 115, 203, 1088, 1168
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 2 cut(s) 457, 1077
Bst4CI ACNGT 2 cut(s) 25, 698
Bst6I CTCTTC 2 cut(s) 177, 206
BstAFI CTTAAG 1 cut(s) 1340
BstAPI GCANNNNNTGC 1 cut(s) 576
BstC8I GCNNGC 2 cut(s) 539, 1266
BstDEI CTNAG 5 cut(s) 40, 1095, 1146, 1152, 1310
BstDSI CCRYGG 1 cut(s) 682
BstEII GGTNACC 1 cut(s) 1115
BstF5I GGATG 5 cut(s) 361, 478, 728, 738, 1351
BstFNI CGCG 1 cut(s) 135
BstHHI GCGC 1 cut(s) 1476
BstKTI GATC 4 cut(s) 118, 206, 1091, 1171
BstMAI GTCTC 4 cut(s) 96, 617, 1142, 1312
BstMBI GATC 4 cut(s) 115, 203, 1088, 1168
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 6 cut(s) 222, 430, 534, 576, 678, 1043
BstNI CCWGG 2 cut(s) 457, 1077
BstNSI RCATGY 3 cut(s) 344, 622, 638
BstPI GGTNACC 1 cut(s) 1115
BstSCI CCNGG 3 cut(s) 455, 507, 1075
BstSFI CTRYAG 1 cut(s) 697
BstSLI GKGCMC 1 cut(s) 1403
BstUI CGCG 1 cut(s) 135
BstV2I GAAGAC 2 cut(s) 26, 1312
BstXI CCANNNNNNTGG 1 cut(s) 1112
BstZI CGGCCG 1 cut(s) 223
Bsu36I CCTNAGG 1 cut(s) 1095
BsuRI GGCC 4 cut(s) 225, 403, 555, 727
BtgI CCRYGG 1 cut(s) 682
BtsCI GGATG 5 cut(s) 361, 478, 728, 738, 1351
BtsIMutI CAGTG 3 cut(s) 327, 444, 1287
Cac8I GCNNGC 2 cut(s) 539, 1266
CfoI GCGC 1 cut(s) 1476
Cfr13I GGNCC 3 cut(s) 245, 269, 553
CseI GACGC 1 cut(s) 1329
Csp6I GTAC 4 cut(s) 97, 231, 367, 1067
CviAII CATG 6 cut(s) 341, 370, 425, 619, 635, 894
CviQI GTAC 4 cut(s) 97, 231, 367, 1067
DdeI CTNAG 5 cut(s) 40, 1095, 1146, 1152, 1310
DpnI GATC 4 cut(s) 117, 205, 1090, 1170
DpnII GATC 4 cut(s) 115, 203, 1088, 1168
DrdI GACNNNNNNGTC 1 cut(s) 276
DseDI GACNNNNNNGTC 1 cut(s) 276
EaeI YGGCCR 1 cut(s) 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 177, 206
EarI CTCTTC 2 cut(s) 177, 206
Ecl136II GAGCTC 1 cut(s) 1408
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 589
Eco24I GRGCYC 1 cut(s) 1410
Eco32I GATATC 3 cut(s) 166, 756, 994
Eco47I GGWCC 2 cut(s) 245, 269
Eco52I CGGCCG 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 1408
Eco57I CTGAAG 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 1095
Eco91I GGTNACC 1 cut(s) 1115
EcoICRI GAGCTC 1 cut(s) 1408
EcoO109I RGGNCCY 1 cut(s) 269
EcoO65I GGTNACC 1 cut(s) 1115
EcoRII CCWGG 2 cut(s) 455, 1075
EcoRV GATATC 3 cut(s) 166, 756, 994
EcoT14I CCWWGG 1 cut(s) 589
EcoT22I ATGCAT 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 1410
ErhI CCWWGG 1 cut(s) 589
Esp3I CGTCTC 1 cut(s) 1312
FaeI CATG 6 cut(s) 344, 373, 428, 622, 638, 897
FatI CATG 6 cut(s) 340, 369, 424, 618, 634, 893
FokI GGATG 5 cut(s) 368, 485, 725, 735, 1358
FriOI GRGCYC 1 cut(s) 1410
FspBI CTAG 5 cut(s) 32, 288, 764, 1013, 1242
GlaI GCGC 1 cut(s) 1475
GsuI CTGGAG 1 cut(s) 407
HaeIII GGCC 4 cut(s) 225, 403, 555, 727
HapII CCGG 3 cut(s) 222, 404, 509
HgaI GACGC 1 cut(s) 1329
HhaI GCGC 1 cut(s) 1476
Hin1II CATG 6 cut(s) 344, 373, 428, 622, 638, 897
Hin6I GCGC 1 cut(s) 1474
HinP1I GCGC 1 cut(s) 1474
HincII GTYRAC 1 cut(s) 1102
HindII GTYRAC 1 cut(s) 1102
HindIII AAGCTT 2 cut(s) 1136, 1337
HinfI GANTC 5 cut(s) 35, 120, 460, 655, 818
HpaII CCGG 3 cut(s) 222, 404, 509
HphI GGTGA 3 cut(s) 1083, 1127, 1190
Hpy166II GTNNAC 1 cut(s) 1102
Hpy188I TCNGA 7 cut(s) 125, 654, 711, 823, 1060, 1147, 1255
Hpy8I GTNNAC 1 cut(s) 1102
Hpy99I CGWCG 1 cut(s) 282
HpyAV CCTTC 3 cut(s) 346, 386, 976
HpyCH4III ACNGT 2 cut(s) 25, 698
HpyCH4IV ACGT 1 cut(s) 1420
HpyF10VI GCNNNNNNNGC 6 cut(s) 222, 430, 534, 576, 678, 1043
HpyF3I CTNAG 5 cut(s) 40, 1095, 1146, 1152, 1310
HpySE526I ACGT 1 cut(s) 1420
Hsp92II CATG 6 cut(s) 344, 373, 428, 622, 638, 897
HspAI GCGC 1 cut(s) 1474
KpnI GGTACC 1 cut(s) 1070
Kzo9I GATC 4 cut(s) 115, 203, 1088, 1168
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 2 cut(s) 421, 929
LweI GCATC 5 cut(s) 165, 225, 747, 877, 899
MaeI CTAG 5 cut(s) 32, 288, 764, 1013, 1242
MaeII ACGT 1 cut(s) 1420
MaeIII GTNAC 2 cut(s) 261, 1115
MalI GATC 4 cut(s) 117, 205, 1090, 1170
MboI GATC 4 cut(s) 115, 203, 1088, 1168
MhlI GDGCHC 3 cut(s) 75, 1403, 1410
MluCI AATT 5 cut(s) 639, 687, 872, 903, 1374
MlyI GAGTC 1 cut(s) 454
MmeI TCCRAC 2 cut(s) 689, 968
Mph1103I ATGCAT 1 cut(s) 892
MseI TTAA 3 cut(s) 972, 1020, 1341
MspCI CTTAAG 1 cut(s) 1340
MspI CCGG 3 cut(s) 222, 404, 509
MspR9I CCNGG 3 cut(s) 457, 509, 1077
Mva1269I GAATGC 2 cut(s) 147, 316
MvaI CCWGG 2 cut(s) 457, 1077
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 6 cut(s) 222, 430, 534, 576, 678, 1043
NciI CCSGG 1 cut(s) 509
NdeII GATC 4 cut(s) 115, 203, 1088, 1168
NlaIII CATG 6 cut(s) 344, 373, 428, 622, 638, 897
NlaIV GGNNCC 4 cut(s) 247, 271, 587, 1068
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 261, 1115
NsiI ATGCAT 1 cut(s) 892
NspI RCATGY 3 cut(s) 344, 622, 638
PciI ACATGT 1 cut(s) 634
PciSI GCTCTTC 1 cut(s) 206
PctI GAATGC 2 cut(s) 147, 316
PfeI GAWTC 4 cut(s) 35, 120, 655, 818
PflMI CCANNNNNTGG 1 cut(s) 1076
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 269
PscI ACATGT 1 cut(s) 634
Psp124BI GAGCTC 1 cut(s) 1410
Psp5II RGGWCCY 1 cut(s) 269
Psp6I CCWGG 2 cut(s) 455, 1075
PspEI GGTNACC 1 cut(s) 1115
PspGI CCWGG 2 cut(s) 455, 1075
PspN4I GGNNCC 4 cut(s) 247, 271, 587, 1068
PspPI GGNCC 3 cut(s) 245, 269, 553
PspPPI RGGWCCY 1 cut(s) 269
RsaI GTAC 4 cut(s) 98, 232, 368, 1068
RsaNI GTAC 4 cut(s) 97, 231, 367, 1067
SacI GAGCTC 1 cut(s) 1410
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 3 cut(s) 972, 1020, 1341
Sau3AI GATC 4 cut(s) 115, 203, 1088, 1168
Sau96I GGNCC 3 cut(s) 245, 269, 553
SchI GAGTC 1 cut(s) 454
ScrFI CCNGG 3 cut(s) 457, 509, 1077
SduI GDGCHC 3 cut(s) 75, 1403, 1410
SfaNI GCATC 5 cut(s) 165, 225, 747, 877, 899
SfcI CTRYAG 1 cut(s) 697
SinI GGWCC 2 cut(s) 245, 269
SmlI CTYRAG 2 cut(s) 1340, 1409
SmoI CTYRAG 2 cut(s) 1340, 1409
Sse9I AATT 5 cut(s) 639, 687, 872, 903, 1374
SsiI CCGC 2 cut(s) 133, 496
SspMI CTAG 5 cut(s) 32, 288, 764, 1013, 1242
SstI GAGCTC 1 cut(s) 1410
StyD4I CCNGG 3 cut(s) 455, 507, 1075
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 2 cut(s) 25, 698
TaiI ACGT 1 cut(s) 1423
TaqI TCGA 5 cut(s) 118, 197, 347, 909, 996
TaqII GACCGA 1 cut(s) 249
TasI AATT 5 cut(s) 639, 687, 872, 903, 1374
TfiI GAWTC 4 cut(s) 35, 120, 655, 818
Tru1I TTAA 3 cut(s) 972, 1020, 1341
Tru9I TTAA 3 cut(s) 972, 1020, 1341
TscAI CASTG 3 cut(s) 327, 451, 1294
TseFI GTSAC 2 cut(s) 261, 1115
Tsp45I GTSAC 2 cut(s) 261, 1115
TspDTI ATGAA 3 cut(s) 32, 386, 1226
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 3 cut(s) 327, 451, 1294
Van91I CCANNNNNTGG 1 cut(s) 1076
Vha464I CTTAAG 1 cut(s) 1340
VpaK11BI GGWCC 2 cut(s) 245, 269
XapI RAATTY 2 cut(s) 687, 1374
XbaI TCTAGA 2 cut(s) 287, 1012
XceI RCATGY 3 cut(s) 344, 622, 638
XspI CTAG 5 cut(s) 32, 288, 764, 1013, 1242
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.