Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001920

Physical Location & Seq

Genomic Coordinates Reverse (-)

9901659 .. 9907770

Gene Structure 6112 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0116800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGCGGAACCGACGTCGTCGGAGCAGATAGTCTATCTGCACGGCGACCTGGACCTCCAAATCATCGAGGCGCGCTACCTGCCCAACATGGACATCTTCTCCGAGCGCCTCCGCCGCTGCTTCACAGCCTGCGACACCGTCAACTGCACCGGCTCCCGCTCCGACAATCCCGATGCCGGCCACGGAGCCGAGGACCGCAAGAAGGTCCACCACCGCAAAATCATCACCAGCGACTCTTACGTCAGCGTCGTCGTGCCCCAGGCCACGGTGGCGCGTACGCGCGTCATCAAGAACTCGCAGAATCCTCAGTGGAAAGAGTCCTTTTCCATCCCGCTCGCGCATCCCGCGGCTAAGCTTGAGTTTCGGGTCAAAGATAACGACATCTTCGGCGCTGAGCTTATCGGCACCGCCTCGATCCCCGCCGCCGAAATCGCCGCCGGAAATGAAATTTCCGGCTGGTATGAGATTATCGGACCCACCGGAAAGCCTCCGAAGCCTGATTGCGCTATCATGGTCAAGTTGAAGTTCACGCCCTTCGAGATAAACCCGGTCTACAGGCACGGCATCGCCGGCGATCCAGAACGGAGGGGAGTCCCCAAGACCTACTTCCCTATGAGGAAGGGAAGTCACGTGAGGCTGTACCAGGACGCGCACGTGCCGGAGGGGTTGCTGCCGCAGATTGAATTGGACGGAAGGAAGGTTTATAAGCCTGAGAATTGCTGGGAGGACATTTGCTATGCGATTTCGGAGGCTCACCATATGATCTACGTGGTGGGATGGTCTGTGTTTCACAAAGTGAGGCTGGTTAGGGAGCCAAGCCGGAAGTTGCCACGCGGCGGCGATTTGATGCTCGGAGATTTGCTCAAGTATAAGTCGGAAGAAGGCGTACGGGTGTTGTTGCTGGTCTGGGATGATAAGACCTCCCACGACAAGTTCTTCTTCAAATCGGCAGGAATGATGCAGACTCATGATGAAGAAACCCGGAAGTTTTTCAAGCATTCATCTGTCACTTGTGTTCTGGCCCCTCGCTATGGCAGTAGTAAGCTTAGCATTATGAAACAACAGGCTGTTGGATCCCTCTTTACACACCATCAAAAATGTGTTATTGTGGACACACAAGCATCTGGTAATAACCGCAAGATAACTACATTTTTAGGAGGTCTTGATCTCTGTGATGGTCGTTATGACACACCTGAGCACAGATTGTTTCGGGATCTCGACACTGTGTTTAAAGAAGATTTTCATCAACCTACGTTTCCTGCTGGGACCAAGGCTCCAAGGCAACCATGGCATGACTTGCACTGTAGAATTGACGGGCCTGCTGCATATGATGTTCTTATAAACTTTGAGCAACGGTGGAGGAGAGCAACACGGTGGAGGGAGTTTGGACTACGTAAAAAGGTTTCGCATTGGCATGATGATGCTTTAATTAAGATAGACCGTATTTCATGGATACTTAGTCCTAAGATGTCTCATTCAAAGGAGTCAAAGGATGTTACAATTGTTCCCGAAGATGACCCAGCTGTATGGGTTCAGAGAGAAGATGATCCCGAAGATTGGCATGTTCAGATTTTCAGGTCTATTGACTCGGGATCACTGAAAGGATTTCCCAAAGATGTCATTCATGCTCAGTCAAAGAACCTTATTTGCTCAAAGGATCTAGTAATAGATAAAAGCATCCAGAAAGCATACATCAATGCAATCAGATCTGCCCAACATTTCATATATATTGAAAATCAGTATTTTCTTGGTTCATCATATGCATGGCCAGATTATAAAAATGCAGGAGCTGACAATCTTATCCCAATGGAGTTGGCACTAAAGATTGCCAGCAAAATTAGAGCTAACGAGAGATTTGCAGTGTATATAGTTCTACCTATGTGGCCTGAAGGTGATCCGAAAACTGGTGCAATGCAAGAAATTCTCTACTGGCAGAGCCAAACAATGCAAATGATGTATGGTGTTGTTGCACGGGAATTGAGGGCCCTGCAGCTTCAGGACTTACATCCTCAAGATTTCCTCAATTTCTATTGCCTTGGTAATCGGGAAAAACTTTCTGAGGAAACATCAAACAACAATGGTGCTACGATCTCCGATGCATACAAGTACCAGCGCTTTATGATTTATGTTCATGCCAAGGGTATGATAGTAGATGACGAGTATGTTATAGTGGGATCTGCCAATATCAACCAAAGATCAATGGCCGGTACAAAAGACACTGAGATAGCTATGGGTGCGTATCAACCCCATCACAGTTGGGCTGAGAAGAAGAGACATCCATGTGGTCAGATATATGGGTATCGAATGTCGCTCTGGGCTGAGCATCTTGGTGAGTTGGACCCATGCTTCAAAGAGGCAGAGAGTTTGGAATGTGTGAGGAATGTGAATGGCATTGCCGAAGAAAACTGGAGGAGATACACATCGCCGGATTTCACAGAACTGCAGGGTCACCTTCTCAGGTATCCGTTGCAGGTAGATGGTGATGGTAACGTGGGACCCCTGCCAGGATATGAAAATTTCCCTGATGTTGGTGGCAAAGTTATTGGAGCTCATTCTGCTGCACTTCCTGATCAATTGACAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-4CXXC_R1

Protein Analysis

861

Amino Acids

98.1

Weight (kDa)

6.73

Isoelectric Point (pI)

35.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	75 - 155	1.5e-15	C2 domain
PLDc_2	PF13091	701 - 744	1.1e-06	PLD-like domain
PLDc	PF00614	708 - 733	3.2e-08	Phospholipase D Active site motif
PLD_C	PF12357	781 - 850	4.4e-28	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001920)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37110 AT4G37110 AT4G37110 AT4G37110
fragaria_vesca	FvH4_1g14770 FvH4_1g14770 FvH4_1g14770 FvH4_2g07200 FvH4_2g07200
malus_domestica	MD02G1160900.v1.1 MD05G1072600.v1.1 MD10G1087200.v1.1 MD10G1087400.v1.1 MD15G1275000.v1.1
prunus_persica	Prupe.7G142700_v2.0.a1 Prupe.7G142700_v2.0.a1 Prupe.8G113300_v2.0.a1 Prupe.8G113300_v2.0.a1 Prupe.8G113400_v2.0.a1 Prupe.8G113400_v2.0.a1
pyrus_communis	pycom02g12700 pycom05g06480 pycom10g06970 pycom10g08140 pycom15g23810
rosa_chinensis	RchiOBHm_Chr2g0103711 RchiOBHm_Chr6g0259631 RchiOBHm_Chr6g0259651
rosa_laevigata	RLG00000014516 RLG00000017251
rosa_multiflora	Rmu_sc0000536.1_g000015
rosa_roxburghii	Rroxscaffold_2G00139460 Rroxscaffold_7G00206930
rosa_rugosa	Rorug02G0116800 Rorug05G0590100
rosa_samantha	Rh2AG165600 Rh2BG172900 Rh2CG171600 Rh2DG171200 Rh6AG106300 Rh6BG100800 Rh6BG100900 Rh6CG095400 Rh6DG089300
rosa_wichuraiana	Rw2G012970 Rw6G009190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	705, 1340, 1776
AasI	GACNNNNNNGTC	1 cut(s)	239
AatII	GACGTC	1 cut(s)	17
Acc36I	ACCTGC	2 cut(s)	87, 2461
AccB1I	GGYRCC	1 cut(s)	404
AccBSI	CCGCTC	2 cut(s)	159, 334
AccI	GTMKAC	1 cut(s)	552
AccII	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
AcoI	YGGCCR	3 cut(s)	178, 1766, 2202
AcsI	RAATTY	3 cut(s)	447, 1920, 2515
AcuI	CTGAAG	2 cut(s)	1908, 1979
AcvI	CACGTG	2 cut(s)	631, 655
AcyI	GRCGYC	1 cut(s)	14
AdeI	CACNNNGTG	1 cut(s)	796
AfaI	GTAC	5 cut(s)	277, 641, 888, 2108, 2209
AfeI	AGCGCT	1 cut(s)	2114
AfiI	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2504, 2528
AgsI	TTSAA	7 cut(s)	523, 683, 943, 994, 1479, 1733, 2350
AjnI	CCWGG	4 cut(s)	48, 258, 642, 2503
AjuI	GAANNNNNNNTTGG	2 cut(s)	590, 622
AluBI	AGCT	9 cut(s)	355, 397, 1045, 1523, 1790, 1844, 1993, 2228, 2549
AluI	AGCT	9 cut(s)	355, 397, 1045, 1523, 1790, 1844, 1993, 2228, 2549
Alw21I	GWGCWC	2 cut(s)	1200, 2551
Alw26I	GTCTC	2 cut(s)	1476, 2266
AlwNI	CAGNNNCTG	1 cut(s)	1790
Ama87I	CYCGRG	1 cut(s)	1588
Aor51HI	AGCGCT	1 cut(s)	2114
AoxI	GGCC	8 cut(s)	178, 261, 1020, 1316, 1766, 1883, 1983, 2202
ApaI	GGGCCC	1 cut(s)	1987
ApeKI	GCWGC	5 cut(s)	117, 670, 1322, 1990, 2558
ApoI	RAATTY	3 cut(s)	447, 1920, 2515
Asp700I	GAANNNNTTC	2 cut(s)	989, 1239
AsuC2I	CCSGG	2 cut(s)	548, 982
AsuHPI	GGTGA	6 cut(s)	217, 746, 1904, 2342, 2441, 2492
AvaI	CYCGRG	1 cut(s)	1588
AvaII	GGWCC	7 cut(s)	52, 193, 205, 473, 1266, 2338, 2495
BaeGI	GKGCMC	2 cut(s)	258, 1987
BalI	TGGCCA	1 cut(s)	1768
BamHI	GGATCC	1 cut(s)	1073
BanI	GGYRCC	1 cut(s)	404
BanII	GRGCYC	2 cut(s)	1987, 2551
BarI	GAAGNNNNNNTAC	2 cut(s)	870, 902
BbrPI	CACGTG	2 cut(s)	631, 655
Bbv12I	GWGCWC	2 cut(s)	1200, 2551
BbvI	GCAGC	5 cut(s)	104, 657, 1309, 2002, 2545
BccI	CCATC	7 cut(s)	335, 771, 1098, 1169, 2256, 2471, 2477
BceAI	ACGGC	2 cut(s)	58, 577
BcgI	CGANNNNNNTGC	2 cut(s)	1838, 1872
BciT130I	CCWGG	4 cut(s)	50, 260, 644, 2505
BciVI	GTATCC	2 cut(s)	1446, 2472
BclI	TGATCA	1 cut(s)	2569
BcnI	CCSGG	2 cut(s)	548, 982
BcoDI	GTCTC	2 cut(s)	1476, 2266
BfaI	CTAG	1 cut(s)	1661
BfmI	CTRYAG	4 cut(s)	553, 1303, 1988, 2441
BfoI	RGCGCY	3 cut(s)	109, 393, 2116
BfuAI	ACCTGC	2 cut(s)	87, 2461
BfuI	GTATCC	2 cut(s)	1446, 2472
BglI	GCCNNNNNGGC	1 cut(s)	269
BglII	AGATCT	1 cut(s)	1706
BlpI	GCTNAGC	4 cut(s)	351, 393, 1046, 2319
Bme1390I	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2505
Bme18I	GGWCC	7 cut(s)	52, 193, 205, 473, 1266, 2338, 2495
BmeT110I	CYCGRG	1 cut(s)	1588
BmrFI	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2505
BplI	GAGNNNNNCTC	2 cut(s)	846, 878
BpmI	CTGGAG	1 cut(s)	2428
Bpu10I	CCTNAGC	1 cut(s)	1194
Bpu1102I	GCTNAGC	4 cut(s)	351, 393, 1046, 2319
BpuEI	CTTGAG	3 cut(s)	377, 848, 1995
BpuMI	CCSGG	2 cut(s)	548, 982
BsaAI	YACGTR	4 cut(s)	631, 655, 769, 1394
BsaBI	GATNNNNATC	2 cut(s)	1242, 2419
BsaHI	GRCGYC	1 cut(s)	14
BsaWI	WCCGGW	1 cut(s)	479
BsaXI	ACNNNNNCTCC	4 cut(s)	83, 113, 1258, 1288
Bsc4I	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2504, 2528
Bse118I	RCCGGY	4 cut(s)	149, 176, 569, 2204
Bse1I	ACTGG	3 cut(s)	1909, 1934, 2411
Bse3DI	GCAATG	2 cut(s)	1917, 2391
Bse8I	GATNNNNATC	2 cut(s)	1242, 2419
BseBI	CCWGG	4 cut(s)	50, 260, 644, 2505
BseGI	GGATG	8 cut(s)	327, 340, 782, 916, 1498, 1677, 2005, 2275
BseJI	GATNNNNATC	2 cut(s)	1242, 2419
BseLI	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2504, 2528
BseMI	GCAATG	2 cut(s)	1917, 2391
BseNI	ACTGG	3 cut(s)	1909, 1934, 2411
BsePI	GCGCGC	1 cut(s)	71
BseRI	GAGGAG	2 cut(s)	1375, 2425
BseSI	GKGCMC	2 cut(s)	258, 1987
BseXI	GCAGC	5 cut(s)	104, 657, 1309, 2002, 2545
BseYI	CCCAGC	3 cut(s)	720, 1262, 1519
BsgI	GTGCAG	3 cut(s)	23, 130, 2544
Bsh1236I	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BshFI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
BshNI	GGYRCC	1 cut(s)	404
BsiHKAI	GWGCWC	2 cut(s)	1200, 2551
BsiHKCI	CYCGRG	1 cut(s)	1588
BsiWI	CGTACG	2 cut(s)	275, 886
BslFI	GGGAC	3 cut(s)	578, 1279, 2508
BslI	CCNNNNNNNGG	9 cut(s)	176, 202, 265, 836, 1031, 1557, 1904, 2504, 2528
BsmAI	GTCTC	2 cut(s)	1476, 2266
BsmFI	GGGAC	3 cut(s)	578, 1279, 2508
BsmI	GAATGC	1 cut(s)	997
BsnI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
BsoBI	CYCGRG	1 cut(s)	1588
Bsp120I	GGGCCC	1 cut(s)	1983
Bsp1286I	GDGCHC	4 cut(s)	258, 1200, 1987, 2551
Bsp1720I	GCTNAGC	4 cut(s)	351, 393, 1046, 2319
Bsp19I	CCATGG	1 cut(s)	1286
BspANI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
BspFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BspHI	TCATGA	1 cut(s)	967
BspMAI	CTGCAG	2 cut(s)	1992, 2445
BspMI	ACCTGC	2 cut(s)	87, 2461
BspT107I	GGYRCC	1 cut(s)	404
BsrBI	CCGCTC	2 cut(s)	159, 334
BsrDI	GCAATG	2 cut(s)	1917, 2391
BsrFI	RCCGGY	4 cut(s)	149, 176, 569, 2204
BsrI	ACTGG	3 cut(s)	1909, 1934, 2411
BssAI	RCCGGY	4 cut(s)	149, 176, 569, 2204
BssHII	GCGCGC	1 cut(s)	71
BssNI	GRCGYC	1 cut(s)	14
BssT1I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
Bst2UI	CCWGG	4 cut(s)	50, 260, 644, 2505
Bst4CI	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2255
Bst6I	CTCTTC	1 cut(s)	2264
BstACI	GRCGYC	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	1297
BstBAI	YACGTR	4 cut(s)	631, 655, 769, 1394
BstC8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1320, 1831
BstDSI	CCRYGG	4 cut(s)	181, 264, 345, 1286
BstEII	GGTNACC	1 cut(s)	2447
BstF5I	GGATG	8 cut(s)	327, 340, 782, 916, 1498, 1677, 2005, 2275
BstFNI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstH2I	RGCGCY	3 cut(s)	109, 393, 2116
BstMAI	GTCTC	2 cut(s)	1476, 2266
BstNI	CCWGG	4 cut(s)	50, 260, 644, 2505
BstNSI	RCATGY	1 cut(s)	1565
BstPI	GGTNACC	1 cut(s)	2447
BstSCI	CCNGG	6 cut(s)	48, 258, 546, 642, 980, 2503
BstSFI	CTRYAG	4 cut(s)	553, 1303, 1988, 2441
BstSLI	GKGCMC	2 cut(s)	258, 1987
BstSNI	TACGTA	1 cut(s)	1394
BstUI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
BstV1I	GCAGC	5 cut(s)	104, 657, 1309, 2002, 2545
BstX2I	RGATCY	5 cut(s)	1073, 1213, 1657, 1706, 2174
BstXI	CCANNNNNNTGG	1 cut(s)	1527
BstYI	RGATCY	5 cut(s)	1073, 1213, 1657, 1706, 2174
BsuI	GTATCC	2 cut(s)	1446, 2472
BsuRI	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
BtgI	CCRYGG	4 cut(s)	181, 264, 345, 1286
BtgZI	GCGATG	2 cut(s)	550, 2406
BtsCI	GGATG	8 cut(s)	327, 340, 782, 916, 1498, 1677, 2005, 2275
BtsI	GCAGTG	1 cut(s)	1866
BtsIMutI	CAGTG	6 cut(s)	314, 1221, 1300, 1595, 1866, 2217
BveI	ACCTGC	2 cut(s)	87, 2461
Cac8I	GCNNGC	7 cut(s)	73, 130, 178, 336, 571, 1320, 1831
CaiI	CAGNNNCTG	1 cut(s)	1790
CciI	TCATGA	1 cut(s)	967
Cfr10I	RCCGGY	4 cut(s)	149, 176, 569, 2204
Cfr42I	CCGCGG	1 cut(s)	348
CseI	GACGC	3 cut(s)	235, 271, 656
Csp6I	GTAC	5 cut(s)	276, 640, 887, 2107, 2208
CviQI	GTAC	5 cut(s)	276, 640, 887, 2107, 2208
DraI	TTTAAA	1 cut(s)	1231
DraIII	CACNNNGTG	1 cut(s)	796
DrdI	GACNNNNNNGTC	1 cut(s)	239
DseDI	GACNNNNNNGTC	1 cut(s)	239
EaeI	YGGCCR	3 cut(s)	178, 1766, 2202
Eam1104I	CTCTTC	1 cut(s)	2264
EarI	CTCTTC	1 cut(s)	2264
EciI	GGCGGA	2 cut(s)	20, 101
Ecl136II	GAGCTC	1 cut(s)	2549
Eco105I	TACGTA	1 cut(s)	1394
Eco130I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
Eco24I	GRGCYC	2 cut(s)	1987, 2551
Eco47I	GGWCC	7 cut(s)	52, 193, 205, 473, 1266, 2338, 2495
Eco47III	AGCGCT	1 cut(s)	2114
Eco53kI	GAGCTC	1 cut(s)	2549
Eco57I	CTGAAG	2 cut(s)	1908, 1979
Eco72I	CACGTG	2 cut(s)	631, 655
Eco88I	CYCGRG	1 cut(s)	1588
Eco91I	GGTNACC	1 cut(s)	2447
EcoICRI	GAGCTC	1 cut(s)	2549
EcoO109I	RGGNCCY	3 cut(s)	1983, 1984, 2495
EcoO65I	GGTNACC	1 cut(s)	2447
EcoRII	CCWGG	4 cut(s)	48, 258, 642, 2503
EcoT14I	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
EcoT22I	ATGCAT	2 cut(s)	1765, 2101
EcoT38I	GRGCYC	2 cut(s)	1987, 2551
ErhI	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
FalI	AAGNNNNNCTT	4 cut(s)	590, 622, 923, 955
FaqI	GGGAC	3 cut(s)	578, 1279, 2508
FauI	CCCGC	4 cut(s)	164, 339, 352, 427
FauNDI	CATATG	3 cut(s)	759, 1327, 1759
FbaI	TGATCA	1 cut(s)	2569
FblI	GTMKAC	1 cut(s)	552
FokI	GGATG	8 cut(s)	314, 327, 789, 923, 1505, 1664, 1992, 2262
FriOI	GRGCYC	2 cut(s)	1987, 2551
FspBI	CTAG	1 cut(s)	1661
GsaI	CCCAGC	3 cut(s)	724, 1266, 1523
GsuI	CTGGAG	1 cut(s)	2428
HaeII	RGCGCY	3 cut(s)	109, 393, 2116
HaeIII	GGCC	8 cut(s)	180, 263, 1022, 1318, 1768, 1885, 1985, 2204
HgaI	GACGC	3 cut(s)	235, 271, 656
Hin1I	GRCGYC	1 cut(s)	14
HincII	GTYRAC	1 cut(s)	142
HindII	GTYRAC	1 cut(s)	142
HindIII	AAGCTT	2 cut(s)	353, 1043
HinfI	GANTC	7 cut(s)	233, 301, 317, 591, 964, 1484, 1586
HphI	GGTGA	6 cut(s)	217, 746, 1904, 2342, 2441, 2492
Hpy166II	GTNNAC	5 cut(s)	142, 208, 528, 553, 1111
Hpy8I	GTNNAC	5 cut(s)	142, 208, 528, 553, 1111
Hpy99I	CGWCG	5 cut(s)	16, 19, 22, 251, 254
HpyAV	CCTTC	8 cut(s)	196, 544, 613, 687, 691, 875, 1883, 2462
HpyCH4III	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2255
HpyCH4IV	ACGT	8 cut(s)	14, 240, 630, 654, 768, 1253, 1393, 2490
HpySE526I	ACGT	8 cut(s)	14, 240, 630, 654, 768, 1253, 1393, 2490
Hsp92I	GRCGYC	1 cut(s)	14
KflI	GGGWCCC	1 cut(s)	2495
KroI	GCCGGC	2 cut(s)	176, 569
KroNI	GCCGGC	2 cut(s)	178, 571
Ksp22I	TGATCA	1 cut(s)	2569
KspI	CCGCGG	1 cut(s)	348
LmnI	GCTCC	8 cut(s)	22, 158, 164, 185, 811, 1279, 1787, 2546
Lsp1109I	GCAGC	5 cut(s)	104, 657, 1309, 2002, 2545
MaeI	CTAG	1 cut(s)	1661
MaeII	ACGT	8 cut(s)	14, 240, 630, 654, 768, 1253, 1393, 2490
MaeIII	GTNAC	5 cut(s)	626, 1006, 1495, 2447, 2486
MbiI	CCGCTC	2 cut(s)	159, 334
MfeI	CAATTG	2 cut(s)	1500, 2573
MflI	RGATCY	5 cut(s)	1073, 1213, 1657, 1706, 2174
MhlI	GDGCHC	4 cut(s)	258, 1200, 1987, 2551
MlsI	TGGCCA	1 cut(s)	1768
MluNI	TGGCCA	1 cut(s)	1768
MlyI	GAGTC	6 cut(s)	227, 326, 600, 958, 1493, 1580
MmeI	TCCRAC	4 cut(s)	186, 855, 1051, 2316
Mox20I	TGGCCA	1 cut(s)	1768
Mph1103I	ATGCAT	2 cut(s)	1765, 2101
MreI	CGCCGGCG	1 cut(s)	569
MroNI	GCCGGC	2 cut(s)	176, 569
MroXI	GAANNNNTTC	2 cut(s)	989, 1239
MscI	TGGCCA	1 cut(s)	1768
MseI	TTAA	3 cut(s)	1230, 1427, 1431
MslI	CAYNNNNRTG	5 cut(s)	1413, 1419, 1762, 2280, 2328
Msp20I	TGGCCA	1 cut(s)	1768
MspA1I	CMGCKG	3 cut(s)	117, 347, 1523
MspR9I	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2505
MunI	CAATTG	2 cut(s)	1500, 2573
Mva1269I	GAATGC	1 cut(s)	997
MvaI	CCWGG	4 cut(s)	50, 260, 644, 2505
MvnI	CGCG	8 cut(s)	73, 274, 280, 282, 338, 347, 650, 834
NaeI	GCCGGC	2 cut(s)	178, 571
NciI	CCSGG	2 cut(s)	548, 982
NcoI	CCATGG	1 cut(s)	1286
NdeI	CATATG	3 cut(s)	759, 1327, 1759
NgoMIV	GCCGGC	2 cut(s)	176, 569
NmeAIII	GCCGAG	1 cut(s)	214
NmuCI	GTSAC	3 cut(s)	626, 1006, 2447
NsiI	ATGCAT	2 cut(s)	1765, 2101
NspI	RCATGY	1 cut(s)	1565
PacI	TTAATTAA	1 cut(s)	1431
PagI	TCATGA	1 cut(s)	967
PauI	GCGCGC	1 cut(s)	71
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PctI	GAATGC	1 cut(s)	997
PdiI	GCCGGC	2 cut(s)	178, 571
PdmI	GAANNNNTTC	2 cut(s)	989, 1239
PfeI	GAWTC	1 cut(s)	301
Pfl23II	CGTACG	2 cut(s)	275, 886
PflFI	GACNNNGTC	2 cut(s)	16, 137
PleI	GAGTC	6 cut(s)	227, 325, 599, 958, 1492, 1580
PmaCI	CACGTG	2 cut(s)	631, 655
PmlI	CACGTG	2 cut(s)	631, 655
PpsI	GAGTC	6 cut(s)	227, 325, 599, 958, 1492, 1580
Ppu21I	YACGTR	4 cut(s)	631, 655, 769, 1394
PpuMI	RGGWCCY	1 cut(s)	2495
PsiI	TTATAA	3 cut(s)	705, 1340, 1776
Psp124BI	GAGCTC	1 cut(s)	2551
Psp5II	RGGWCCY	1 cut(s)	2495
Psp6I	CCWGG	4 cut(s)	48, 258, 642, 2503
PspCI	CACGTG	2 cut(s)	631, 655
PspEI	GGTNACC	1 cut(s)	2447
PspFI	CCCAGC	3 cut(s)	720, 1262, 1519
PspGI	CCWGG	4 cut(s)	48, 258, 642, 2503
PspLI	CGTACG	2 cut(s)	275, 886
PspOMI	GGGCCC	1 cut(s)	1983
PspPPI	RGGWCCY	1 cut(s)	2495
PstI	CTGCAG	2 cut(s)	1992, 2445
PstNI	CAGNNNCTG	1 cut(s)	1790
PsuI	RGATCY	5 cut(s)	1073, 1213, 1657, 1706, 2174
PsyI	GACNNNGTC	2 cut(s)	16, 137
PteI	GCGCGC	1 cut(s)	71
PvuII	CAGCTG	1 cut(s)	1523
RsaI	GTAC	5 cut(s)	277, 641, 888, 2108, 2209
RsaNI	GTAC	5 cut(s)	276, 640, 887, 2107, 2208
RseI	CAYNNNNRTG	5 cut(s)	1413, 1419, 1762, 2280, 2328
SacI	GAGCTC	1 cut(s)	2551
SacII	CCGCGG	1 cut(s)	348
SaqAI	TTAA	3 cut(s)	1230, 1427, 1431
SchI	GAGTC	6 cut(s)	227, 326, 600, 958, 1493, 1580
ScrFI	CCNGG	6 cut(s)	50, 260, 548, 644, 982, 2505
SduI	GDGCHC	4 cut(s)	258, 1200, 1987, 2551
SfcI	CTRYAG	4 cut(s)	553, 1303, 1988, 2441
Sfr303I	CCGCGG	1 cut(s)	348
SgrAI	CRCCGGYG	1 cut(s)	569
SgrBI	CCGCGG	1 cut(s)	348
SinI	GGWCC	7 cut(s)	52, 193, 205, 473, 1266, 2338, 2495
SmiMI	CAYNNNNRTG	5 cut(s)	1413, 1419, 1762, 2280, 2328
SmlI	CTYRAG	3 cut(s)	356, 863, 2010
SmoI	CTYRAG	3 cut(s)	356, 863, 2010
SnaBI	TACGTA	1 cut(s)	1394
SspMI	CTAG	1 cut(s)	1661
SstI	GAGCTC	1 cut(s)	2551
StyD4I	CCNGG	6 cut(s)	48, 258, 546, 642, 980, 2503
StyI	CCWWGG	5 cut(s)	1269, 1277, 1286, 2035, 2136
TaaI	ACNGT	8 cut(s)	139, 268, 1225, 1304, 1356, 1374, 1442, 2255
TaiI	ACGT	8 cut(s)	17, 243, 633, 657, 771, 1256, 1396, 2493
TaqI	TCGA	5 cut(s)	66, 413, 537, 1218, 2302
TauI	GCSGC	7 cut(s)	117, 350, 425, 437, 676, 837, 840
TfiI	GAWTC	1 cut(s)	301
Tru1I	TTAA	3 cut(s)	1230, 1427, 1431
Tru9I	TTAA	3 cut(s)	1230, 1427, 1431
TscAI	CASTG	6 cut(s)	314, 1228, 1307, 1602, 1866, 2224
TseFI	GTSAC	3 cut(s)	626, 1006, 2447
TseI	GCWGC	5 cut(s)	117, 670, 1322, 1990, 2558
Tsp45I	GTSAC	3 cut(s)	626, 1006, 2447
TspGWI	ACGGA	4 cut(s)	198, 598, 705, 2454
TspRI	CASTG	6 cut(s)	314, 1228, 1307, 1602, 1866, 2224
Tth111I	GACNNNGTC	2 cut(s)	16, 137
VpaK11BI	GGWCC	7 cut(s)	52, 193, 205, 473, 1266, 2338, 2495
XapI	RAATTY	3 cut(s)	447, 1920, 2515
XceI	RCATGY	1 cut(s)	1565
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1284
XmiI	GTMKAC	1 cut(s)	552
XmnI	GAANNNNTTC	2 cut(s)	989, 1239
XspI	CTAG	1 cut(s)	1661
ZraI	GACGTC	1 cut(s)	15
Zsp2I	ATGCAT	2 cut(s)	1765, 2101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.