MD15G1275000.v1.1

Cell division cycle-associated protein 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001920
Physical Location & Seq
Reverse (-)
24149038 .. 24152601
3564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1275000.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGGAAATTGAAAAGTTGCGCCCCCCTAGTCTCTCTCCCTTGCTGCAAATAAGGGAAAATCCACAATTCGGGCTCAAAATTCGACCGTTGGAAGCGCAGGATTCAAATCACCCACTGCCACTTAGAATCCAAGAGAGAGATATAAATTCAAATCACCCACACCCACACCCACAGAGACTTGTCAGATTCCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTTCACAATGCCTACTCTGGGAAAGAAGACCGTTGCCCTGGAATCCTCAGCAAACCCTAACCCTAGAAGCAACAATGAGCACCTCCAAACCCCCAAGTCTTTGTACGAGCAGTCCAGAGAAGATCGAATCAGAGAAAACCGCGAAAGAATGCAAAAGCTCGGCATCGTTGACATCTCTCTTCAGCTCAAATCTAACATTCACTCGAAGAGATCAACAGCAAAGAGCTACTCCAACCGCTGTACGACGCCGTCTGGGCCCCCTTCCATTCGGACGCTAGGACCCACTCGTCGTTCTTCTAGATTGAAGAATGCTACACCAGTGAGCTACGCGGAGGTGCATGTGTCGAAGAAGGATGACGCTTCGTACATGAAGGGTATTATGCTGGAGGAGGGTGAAAGGCCGGAGATTTACACGGAGGAGCATGAGAAGCTGTTAGGCAACACTGATAAGACCTGGACTCTGTTTGTGGATGGTTATGGAAAAGATGGAAAACGGATTTATGACCCGGTTAGAGGAAAGACTTGCCATCAATGCAGGCAGAAAACTCTGGGTCATCATACTCAATGTAACCAATGCAACAGGCTCCAAGGACAGTTTTGTGGTGATTGTTTGTACATGAGATATGGGGAACATGTAATTGAAGCCAATCAGAATCCAGATTGGATTTGCCCTGTCTGCCGTGGAATCTGCAACTGTAGTTTTTGTCGGACGAAAAAAGGGTGGCCTCCTACTGGCATCCTCTACAAGAAGATATCGCAACTAGGTTTCAAATCAGTTGCACACTATCTCATTCACACCCAACGGGGACAGACAAATTCAGGAGAAAATCTGGAAACCACCAATCCAGTTTCTGCAAAGAGGTCATTGCAATTCCCAGATGTAGATGCATCATGTGAAGAAATTGTCAAGGACGACAATGATATTGGAGCAATAAAACCTCTGGTTGGGCAAGAAAGAGATGACGAGTTTAAGACTGAGAAGGAGAATGAAATCAAGAGCAGCACAAATCTAGATATTAACAACCAAACTTCTGCTGAGAGGGTATTGTCCTTTTCAGATATGGTACAGCAGTCTGAAAATTTTGGATTGTCTGAGGTCGACCACAAGGTTGGTGACCACCTTGGATTGTCAAAGCTTCAATCTGAAAGTGGCAGAGATGATCGTCAAGGTGAGAAAGAGAATACAATGCCCTTGATGGATATGAAACTTGGTGATAGCAGTAATGCATTGGAAACTAGCTCAAAGTCTAAAATGAAGCGTGCTCTGGATATTGAACCGAGCACTGATAGTATTGGTGGAAGACTGAGGCAGAGACGCAGGAAAGGAAACAAGCTTAGTGATGATGAATTACAAGAGGCAAAAGTGGAAATTCTAGATAACAGTCCTGCATTGGGCCCAAGATCAAGGCTCAGGAAGAAGCATGTTCTTGCTGAACCAAGTCCAGACAGCATTGGTGGAAGATTGAGGCAGAGGCGTAAGGTCAACACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-4CXXC_R1

Protein Analysis

570

Amino Acids

64.82

Weight (kDa)

9.08

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 240 - 336 4.7e-41 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 504
AccI GTMKAC 1 cut(s) 1317
AccII CGCG 2 cut(s) 360, 548
AciI CCGC 3 cut(s) 358, 454, 548
AcsI RAATTY 5 cut(s) 78, 145, 1033, 1297, 1587
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 464
AfaI GTAC 5 cut(s) 323, 460, 584, 833, 1284
AfiI CCNNNNNNNGG 6 cut(s) 68, 236, 731, 950, 1163, 1610
AflIII ACRYGT 2 cut(s) 850, 1704
AgsI TTSAA 8 cut(s) 11, 105, 150, 523, 860, 988, 1358, 1493
AjnI CCWGG 2 cut(s) 255, 671
AluBI AGCT 8 cut(s) 376, 403, 444, 543, 649, 1354, 1458, 1552
AluI AGCT 8 cut(s) 376, 403, 444, 543, 649, 1354, 1458, 1552
Alw21I GWGCWC 3 cut(s) 300, 1483, 1502
Alw26I GTCTC 3 cut(s) 35, 169, 1525
AlwNI CAGNNNCTG 1 cut(s) 1070
AoxI GGCC 4 cut(s) 473, 617, 941, 1612
ApaI GGGCCC 2 cut(s) 477, 1616
ApeKI GCWGC 2 cut(s) 43, 1218
ApoI RAATTY 5 cut(s) 78, 145, 1033, 1297, 1587
AspLEI GCGC 2 cut(s) 21, 97
AspS9I GGNCC 5 cut(s) 473, 474, 497, 1612, 1613
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 7 cut(s) 101, 146, 623, 833, 1343, 1400, 1442
AvaII GGWCC 1 cut(s) 497
BaeGI GKGCMC 2 cut(s) 477, 1616
BaeI ACNNNNGTAYC 2 cut(s) 1274, 1307
BanII GRGCYC 3 cut(s) 75, 477, 1616
BbsI GAAGAC 2 cut(s) 251, 1525
Bbv12I GWGCWC 3 cut(s) 300, 1483, 1502
BbvCI CCTCAGC 1 cut(s) 265
BbvI GCAGC 2 cut(s) 30, 1230
BccI CCATC 4 cut(s) 683, 698, 753, 1408
BceAI ACGGC 2 cut(s) 451, 882
BciT130I CCWGG 2 cut(s) 257, 673
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 3 cut(s) 35, 169, 1525
BfaI CTAG 8 cut(s) 27, 282, 494, 516, 980, 1229, 1455, 1592
BfmI CTRYAG 1 cut(s) 913
BglI GCCNNNNNGGC 1 cut(s) 472
BisI GCNGC 2 cut(s) 44, 1219
BlsI GCNGC 2 cut(s) 45, 1220
Bme1390I CCNGG 3 cut(s) 257, 673, 725
Bme18I GGWCC 1 cut(s) 497
BmgT120I GGNCC 5 cut(s) 473, 474, 497, 1612, 1613
BmiI GGNNCC 5 cut(s) 475, 476, 499, 803, 1614
BmrFI CCNGG 3 cut(s) 257, 673, 725
BmsI GCATC 4 cut(s) 390, 963, 1093, 1115
BpiI GAAGAC 2 cut(s) 251, 1525
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 2 cut(s) 265, 1628
BpuMI CCSGG 1 cut(s) 725
BsaAI YACGTR 1 cut(s) 1707
BsaBI GATNNNNATC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 464
BsaJI CCNNGG 4 cut(s) 255, 805, 898, 1339
Bsc4I CCNNNNNNNGG 6 cut(s) 68, 236, 731, 950, 1163, 1610
Bse1I ACTGG 3 cut(s) 536, 955, 1064
Bse3DI GCAATG 1 cut(s) 1082
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 2 cut(s) 257, 673
BseDI CCNNGG 4 cut(s) 255, 805, 898, 1339
BseGI GGATG 3 cut(s) 577, 694, 954
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 6 cut(s) 68, 236, 731, 950, 1163, 1610
BseMI GCAATG 1 cut(s) 1082
BseMII CTCAG 6 cut(s) 279, 1185, 1245, 1302, 1514, 1642
BseNI ACTGG 3 cut(s) 536, 955, 1064
BseRI GAGGAG 2 cut(s) 620, 650
BseSI GKGCMC 2 cut(s) 477, 1616
BseXI GCAGC 2 cut(s) 30, 1230
Bsh1236I CGCG 2 cut(s) 360, 548
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 4 cut(s) 475, 619, 943, 1614
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 3 cut(s) 300, 1483, 1502
BsiSI CCGG 2 cut(s) 620, 725
BslFI GGGAC 1 cut(s) 1038
BslI CCNNNNNNNGG 6 cut(s) 68, 236, 731, 950, 1163, 1610
BsmAI GTCTC 3 cut(s) 35, 169, 1525
BsmBI CGTCTC 1 cut(s) 1525
BsmFI GGGAC 1 cut(s) 1038
BsmI GAATGC 2 cut(s) 372, 532
BsnI GGCC 4 cut(s) 475, 619, 943, 1614
Bsp120I GGGCCC 2 cut(s) 473, 1612
Bsp1286I GDGCHC 6 cut(s) 75, 300, 477, 1483, 1502, 1616
Bsp1407I TGTACA 1 cut(s) 831
Bsp143I GATC 4 cut(s) 340, 428, 1378, 1619
BspACI CCGC 3 cut(s) 358, 454, 548
BspANI GGCC 4 cut(s) 475, 619, 943, 1614
BspCNI CTCAG 6 cut(s) 278, 1186, 1246, 1303, 1515, 1641
BspFNI CGCG 2 cut(s) 360, 548
BspLI GGNNCC 5 cut(s) 475, 476, 499, 803, 1614
BsrDI GCAATG 1 cut(s) 1082
BsrGI TGTACA 1 cut(s) 831
BsrI ACTGG 3 cut(s) 536, 955, 1064
BssECI CCNNGG 4 cut(s) 255, 805, 898, 1339
BssMI GATC 4 cut(s) 340, 428, 1378, 1619
BssNI GRCGYC 1 cut(s) 464
BssT1I CCWWGG 2 cut(s) 805, 1339
Bst2UI CCWGG 2 cut(s) 257, 673
Bst4CI ACNGT 5 cut(s) 87, 250, 813, 914, 1601
Bst6I CTCTTC 2 cut(s) 402, 419
BstACI GRCGYC 1 cut(s) 464
BstAUI TGTACA 1 cut(s) 831
BstBAI YACGTR 1 cut(s) 1707
BstC8I GCNNGC 2 cut(s) 755, 1479
BstDEI CTNAG 8 cut(s) 122, 265, 1194, 1254, 1311, 1523, 1553, 1628
BstDSI CCRYGG 1 cut(s) 898
BstEII GGTNACC 1 cut(s) 1331
BstF5I GGATG 3 cut(s) 577, 694, 954
BstFNI CGCG 2 cut(s) 360, 548
BstHHI GCGC 2 cut(s) 21, 97
BstKTI GATC 4 cut(s) 343, 431, 1381, 1622
BstMAI GTCTC 3 cut(s) 35, 169, 1525
BstMBI GATC 4 cut(s) 340, 428, 1378, 1619
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 4 cut(s) 472, 646, 750, 894
BstNI CCWGG 2 cut(s) 257, 673
BstNSI RCATGY 3 cut(s) 560, 854, 1643
BstPI GGTNACC 1 cut(s) 1331
BstSCI CCNGG 3 cut(s) 255, 671, 723
BstSFI CTRYAG 1 cut(s) 913
BstSLI GKGCMC 2 cut(s) 477, 1616
BstUI CGCG 2 cut(s) 360, 548
BstV1I GCAGC 2 cut(s) 30, 1230
BstV2I GAAGAC 2 cut(s) 251, 1525
BstXI CCANNNNNNTGG 1 cut(s) 1328
BsuRI GGCC 4 cut(s) 475, 619, 943, 1614
BtgI CCRYGG 1 cut(s) 898
BtsCI GGATG 3 cut(s) 577, 694, 954
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 4 cut(s) 113, 543, 660, 1500
Cac8I GCNNGC 2 cut(s) 755, 1479
CaiI CAGNNNCTG 1 cut(s) 1070
CfoI GCGC 2 cut(s) 21, 97
Cfr13I GGNCC 5 cut(s) 473, 474, 497, 1612, 1613
CseI GACGC 4 cut(s) 472, 499, 584, 1542
Csp6I GTAC 5 cut(s) 322, 459, 583, 832, 1283
CviAII CATG 7 cut(s) 557, 586, 641, 835, 851, 1110, 1640
CviQI GTAC 5 cut(s) 322, 459, 583, 832, 1283
DdeI CTNAG 8 cut(s) 122, 265, 1194, 1254, 1311, 1523, 1553, 1628
DpnI GATC 4 cut(s) 342, 430, 1380, 1621
DpnII GATC 4 cut(s) 340, 428, 1378, 1619
DrdI GACNNNNNNGTC 1 cut(s) 504
DseDI GACNNNNNNGTC 1 cut(s) 504
Eam1104I CTCTTC 2 cut(s) 402, 419
EarI CTCTTC 2 cut(s) 402, 419
Eco130I CCWWGG 2 cut(s) 805, 1339
Eco24I GRGCYC 3 cut(s) 75, 477, 1616
Eco32I GATATC 1 cut(s) 972
Eco47I GGWCC 1 cut(s) 497
Eco57I CTGAAG 1 cut(s) 383
Eco91I GGTNACC 1 cut(s) 1331
EcoO109I RGGNCCY 2 cut(s) 474, 497
EcoO65I GGTNACC 1 cut(s) 1331
EcoRII CCWGG 2 cut(s) 255, 671
EcoRV GATATC 1 cut(s) 972
EcoT14I CCWWGG 2 cut(s) 805, 1339
EcoT22I ATGCAT 2 cut(s) 1108, 1447
EcoT38I GRGCYC 3 cut(s) 75, 477, 1616
ErhI CCWWGG 2 cut(s) 805, 1339
Esp3I CGTCTC 1 cut(s) 1525
FaeI CATG 7 cut(s) 560, 589, 644, 838, 854, 1113, 1643
FaqI GGGAC 1 cut(s) 1038
FatI CATG 7 cut(s) 556, 585, 640, 834, 850, 1109, 1639
FblI GTMKAC 1 cut(s) 1317
Fnu4HI GCNGC 2 cut(s) 44, 1219
FokI GGATG 3 cut(s) 584, 701, 941
FriOI GRGCYC 3 cut(s) 75, 477, 1616
Fsp4HI GCNGC 2 cut(s) 44, 1219
FspBI CTAG 8 cut(s) 27, 282, 494, 516, 980, 1229, 1455, 1592
GlaI GCGC 2 cut(s) 20, 96
GluI GCNGC 2 cut(s) 44, 1219
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 4 cut(s) 475, 619, 943, 1614
HapII CCGG 2 cut(s) 620, 725
HgaI GACGC 4 cut(s) 472, 499, 584, 1542
HhaI GCGC 2 cut(s) 21, 97
Hin1I GRCGYC 1 cut(s) 464
Hin1II CATG 7 cut(s) 560, 589, 644, 838, 854, 1113, 1643
Hin6I GCGC 2 cut(s) 19, 95
HinP1I GCGC 2 cut(s) 19, 95
HincII GTYRAC 3 cut(s) 388, 1318, 1702
HindII GTYRAC 3 cut(s) 388, 1318, 1702
HindIII AAGCTT 2 cut(s) 1352, 1550
HinfI GANTC 8 cut(s) 101, 126, 186, 260, 345, 676, 871, 903
HpaII CCGG 2 cut(s) 620, 725
HphI GGTGA 7 cut(s) 101, 146, 623, 833, 1343, 1400, 1442
Hpy166II GTNNAC 4 cut(s) 222, 388, 1318, 1702
Hpy8I GTNNAC 4 cut(s) 222, 388, 1318, 1702
Hpy99I CGWCG 2 cut(s) 466, 510
HpyAV CCTTC 4 cut(s) 489, 562, 583, 1192
HpyCH4III ACNGT 5 cut(s) 87, 250, 813, 914, 1601
HpyCH4IV ACGT 1 cut(s) 1706
HpyF10VI GCNNNNNNNGC 4 cut(s) 472, 646, 750, 894
HpyF3I CTNAG 8 cut(s) 122, 265, 1194, 1254, 1311, 1523, 1553, 1628
HpySE526I ACGT 1 cut(s) 1706
Hsp92I GRCGYC 1 cut(s) 464
Hsp92II CATG 7 cut(s) 560, 589, 644, 838, 854, 1113, 1643
HspAI GCGC 2 cut(s) 19, 95
Kzo9I GATC 4 cut(s) 340, 428, 1378, 1619
LmnI GCTCC 3 cut(s) 637, 807, 1145
Lsp1109I GCAGC 2 cut(s) 30, 1230
LweI GCATC 4 cut(s) 390, 963, 1093, 1115
MaeI CTAG 8 cut(s) 27, 282, 494, 516, 980, 1229, 1455, 1592
MaeII ACGT 1 cut(s) 1706
MaeIII GTNAC 2 cut(s) 785, 1331
MalI GATC 4 cut(s) 342, 430, 1380, 1621
MboI GATC 4 cut(s) 340, 428, 1378, 1619
MhlI GDGCHC 6 cut(s) 75, 300, 477, 1483, 1502, 1616
MlyI GAGTC 1 cut(s) 670
MmeI TCCRAC 3 cut(s) 69, 474, 905
Mph1103I ATGCAT 2 cut(s) 1108, 1447
MseI TTAA 2 cut(s) 1188, 1236
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 2 cut(s) 620, 725
MspR9I CCNGG 3 cut(s) 257, 673, 725
Mva1269I GAATGC 2 cut(s) 372, 532
MvaI CCWGG 2 cut(s) 257, 673
MvnI CGCG 2 cut(s) 360, 548
MwoI GCNNNNNNNGC 4 cut(s) 472, 646, 750, 894
NciI CCSGG 1 cut(s) 725
NdeII GATC 4 cut(s) 340, 428, 1378, 1619
NlaIII CATG 7 cut(s) 560, 589, 644, 838, 854, 1113, 1643
NlaIV GGNNCC 5 cut(s) 475, 476, 499, 803, 1614
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 1331
NsiI ATGCAT 2 cut(s) 1108, 1447
NspI RCATGY 3 cut(s) 560, 854, 1643
PciI ACATGT 1 cut(s) 850
PctI GAATGC 2 cut(s) 372, 532
PfeI GAWTC 7 cut(s) 101, 126, 186, 260, 345, 871, 903
PflFI GACNNNGTC 1 cut(s) 466
PkrI GCNGC 2 cut(s) 45, 1220
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 1707
PpuMI RGGWCCY 1 cut(s) 497
PscI ACATGT 1 cut(s) 850
Psp5II RGGWCCY 1 cut(s) 497
Psp6I CCWGG 2 cut(s) 255, 671
PspEI GGTNACC 1 cut(s) 1331
PspGI CCWGG 2 cut(s) 255, 671
PspN4I GGNNCC 5 cut(s) 475, 476, 499, 803, 1614
PspOMI GGGCCC 2 cut(s) 473, 1612
PspPI GGNCC 5 cut(s) 473, 474, 497, 1612, 1613
PspPPI RGGWCCY 1 cut(s) 497
PstNI CAGNNNCTG 1 cut(s) 1070
PsyI GACNNNGTC 1 cut(s) 466
RsaI GTAC 5 cut(s) 323, 460, 584, 833, 1284
RsaNI GTAC 5 cut(s) 322, 459, 583, 832, 1283
SalI GTCGAC 1 cut(s) 1316
SaqAI TTAA 2 cut(s) 1188, 1236
SatI GCNGC 2 cut(s) 44, 1219
Sau3AI GATC 4 cut(s) 340, 428, 1378, 1619
Sau96I GGNCC 5 cut(s) 473, 474, 497, 1612, 1613
SchI GAGTC 1 cut(s) 670
ScrFI CCNGG 3 cut(s) 257, 673, 725
SduI GDGCHC 6 cut(s) 75, 300, 477, 1483, 1502, 1616
SfaNI GCATC 4 cut(s) 390, 963, 1093, 1115
SfcI CTRYAG 1 cut(s) 913
SinI GGWCC 1 cut(s) 497
SsiI CCGC 3 cut(s) 358, 454, 548
SspMI CTAG 8 cut(s) 27, 282, 494, 516, 980, 1229, 1455, 1592
StyD4I CCNGG 3 cut(s) 255, 671, 723
StyI CCWWGG 2 cut(s) 805, 1339
TaaI ACNGT 5 cut(s) 87, 250, 813, 914, 1601
TaiI ACGT 1 cut(s) 1709
TaqI TCGA 5 cut(s) 82, 343, 422, 563, 1317
TatI WGTACW 1 cut(s) 831
TfiI GAWTC 7 cut(s) 101, 126, 186, 260, 345, 871, 903
Tru1I TTAA 2 cut(s) 1188, 1236
Tru9I TTAA 2 cut(s) 1188, 1236
TscAI CASTG 4 cut(s) 120, 543, 667, 1507
TseFI GTSAC 1 cut(s) 1331
TseI GCWGC 2 cut(s) 43, 1218
Tsp45I GTSAC 1 cut(s) 1331
TspDTI ATGAA 5 cut(s) 602, 1221, 1436, 1487, 1578
TspGWI ACGGA 2 cut(s) 647, 727
TspRI CASTG 4 cut(s) 120, 543, 667, 1507
Tth111I GACNNNGTC 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 497
XapI RAATTY 5 cut(s) 78, 145, 1033, 1297, 1587
XbaI TCTAGA 3 cut(s) 515, 1228, 1591
XceI RCATGY 3 cut(s) 560, 854, 1643
XmiI GTMKAC 1 cut(s) 1317
XspI CTAG 8 cut(s) 27, 282, 494, 516, 980, 1229, 1455, 1592
Zsp2I ATGCAT 2 cut(s) 1108, 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.