pycom05g06480

Cell division cycle-associated 7-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001920
Physical Location & Seq
Forward (+)
8951329 .. 8953936
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g06480.1

Sequence Viewer

Length: 930 bp
ATGGCGGTTCTGAGAAATCGAGGCGGAGGAAACTGCGGAGAGAACGGCGGAGCAACCGTAGAGAACGAAACCGCAGGGACTTCAGGGTACGAGCAGTTCAGGGACCATAGGATCAGAGAGAACAAGGAGAGATTGCAAAAGCTGGGCATTTTGGACCTTGCCCTGAAGCTCAAGACCGAAGCTGCCGCCCCAAAACGTCCCCACCGGGCAAAAACCCACAGCCCACTTCCTGGTTCCGGATCCCCCAGGCGCTCTTCTAGGTTGAAGAGTGTGGCTCCTGTAGATTATGTGCAAAAGAAGAGGCCTATGAAAAAAGACGAGAACCCAAAGAGCCCAAAGAGGAGGAGTGTTGAAATTTTTATAAAGGAAGGTTCAAAGCCAGAGATCTATACAGAGGAGCATGAGAAGCTGTTAGGGGATTGCGTTGAAAGCTGGGAGTTGGGTGTTGATGGCTATGGGGAAGATGGGAGGCGTATTTACGATCCGGTTAAGGGAGAATTTTGCCACCAATGCAGATATGGAGAGAATGTTATGGAAGCCAGCGAAAATCCTGATTGGACGTGTCCTGTTTGTCGGGGCATCTGTAACTGCAATTGCTGCCGTCAAGCGAAGGGGTGGGAACCCACTGGTAATGTGTATAGAAAGGTCGTAAAACTGGGTTACAAATCTGTGGCACATTATCTCATTCAAAACCGCCATCCCTCAACAAACACAGAAACCACAGGTGAAGAGGTTCTTGCAGAGGGATCAACTCCTTCAGTGGAGACCTTTCCACACAATGAATCTTCAGGAATTGGCAGTGGCTCTGATACTAGCAAGCATGTTGGTGACAACGATGAGGGTAATGCAGATGCTGTTGCTGCTGATGTTGCTGTTGACAATGAGGCAAAGAAAAAGCTGAGAAGGAGTACAAGGCTGAGCGGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-4CXXC_R1

Protein Analysis

310

Amino Acids

34.08

Weight (kDa)

8.44

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 163 - 228 3e-23 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 230
AccBSI CCGCTC 1 cut(s) 921
AccIII TCCGGA 1 cut(s) 236
AciI CCGC 8 cut(s) 5, 24, 36, 48, 72, 186, 694, 921
AclWI GGATC 5 cut(s) 119, 234, 247, 476, 754
AcsI RAATTY 2 cut(s) 354, 497
AcuI CTGAAG 4 cut(s) 66, 185, 741, 771
AfaI GTAC 2 cut(s) 89, 910
AfiI CCNNNNNNNGG 3 cut(s) 230, 236, 491
AflIII ACRYGT 1 cut(s) 560
AgsI TTSAA 5 cut(s) 265, 353, 375, 428, 689
AjiI CACGTC 1 cut(s) 561
AjnI CCWGG 2 cut(s) 229, 245
AluBI AGCT 6 cut(s) 142, 169, 182, 409, 432, 898
AluI AGCT 6 cut(s) 142, 169, 182, 409, 432, 898
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 5 cut(s) 119, 234, 247, 476, 754
AlwNI CAGNNNCTG 1 cut(s) 854
Aor13HI TCCGGA 1 cut(s) 236
AoxI GGCC 1 cut(s) 302
ApeKI GCWGC 3 cut(s) 182, 597, 860
ApoI RAATTY 2 cut(s) 354, 497
Asp700I GAANNNNTTC 1 cut(s) 732
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 103, 154
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 737, 839
AvaII GGWCC 2 cut(s) 103, 154
BamHI GGATCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 335
BbvI GCAGC 3 cut(s) 169, 584, 847
BccI CCATC 3 cut(s) 443, 458, 705
BceAI ACGGC 2 cut(s) 61, 585
BciT130I CCWGG 2 cut(s) 231, 247
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 2 cut(s) 258, 813
BfmI CTRYAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 253
BglII AGATCT 1 cut(s) 384
BisI GCNGC 4 cut(s) 183, 186, 598, 861
BlpI GCTNAGC 1 cut(s) 917
BlsI GCNGC 4 cut(s) 184, 187, 599, 862
Bme1390I CCNGG 3 cut(s) 206, 231, 247
Bme18I GGWCC 2 cut(s) 103, 154
BmgBI CACGTC 1 cut(s) 561
BmgT120I GGNCC 2 cut(s) 103, 154
BmiI GGNNCC 5 cut(s) 104, 235, 241, 276, 621
BmrFI CCNGG 3 cut(s) 206, 231, 247
BmrI ACTGGG 1 cut(s) 665
BmsI GCATC 2 cut(s) 588, 841
BmuI ACTGGG 1 cut(s) 665
BplI GAGNNNNNCTC 2 cut(s) 259, 291
Bpu1102I GCTNAGC 1 cut(s) 917
BpuEI CTTGAG 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 206
BsaI GGTCTC 1 cut(s) 758
BsaJI CCNNGG 1 cut(s) 245
BsaWI WCCGGW 2 cut(s) 236, 484
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 513, 543
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 236, 491
Bse1I ACTGG 2 cut(s) 631, 660
BseAI TCCGGA 1 cut(s) 236
BseBI CCWGG 2 cut(s) 231, 247
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 1 cut(s) 697
BseLI CCNNNNNNNGG 3 cut(s) 230, 236, 491
BseMII CTCAG 2 cut(s) 890, 908
BseNI ACTGG 2 cut(s) 631, 660
BseRI GAGGAG 3 cut(s) 355, 358, 410
BseXI GCAGC 3 cut(s) 169, 584, 847
BseYI CCCAGC 2 cut(s) 142, 432
BshFI GGCC 1 cut(s) 304
BsiSI CCGG 3 cut(s) 205, 237, 485
BslFI GGGAC 3 cut(s) 91, 116, 183
BslI CCNNNNNNNGG 3 cut(s) 230, 236, 491
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 3 cut(s) 91, 116, 183
BsnI GGCC 1 cut(s) 304
Bso31I GGTCTC 1 cut(s) 758
Bsp1286I GDGCHC 1 cut(s) 335
Bsp13I TCCGGA 1 cut(s) 236
Bsp143I GATC 5 cut(s) 111, 239, 384, 481, 746
Bsp1720I GCTNAGC 1 cut(s) 917
BspACI CCGC 8 cut(s) 5, 24, 36, 48, 72, 186, 694, 921
BspANI GGCC 1 cut(s) 304
BspCNI CTCAG 2 cut(s) 891, 909
BspEI TCCGGA 1 cut(s) 236
BspLI GGNNCC 5 cut(s) 104, 235, 241, 276, 621
BspPI GGATC 5 cut(s) 119, 234, 247, 476, 754
BspQI GCTCTTC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 758
BsrBI CCGCTC 1 cut(s) 921
BsrI ACTGG 2 cut(s) 631, 660
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 5 cut(s) 111, 239, 384, 481, 746
Bst2UI CCWGG 2 cut(s) 231, 247
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 4 cut(s) 259, 260, 293, 723
BstAPI GCANNNNNTGC 1 cut(s) 597
BstC8I GCNNGC 2 cut(s) 541, 818
BstDEI CTNAG 3 cut(s) 11, 899, 917
BstF5I GGATG 1 cut(s) 697
BstH2I RGCGCY 1 cut(s) 253
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 5 cut(s) 114, 242, 387, 484, 749
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 5 cut(s) 111, 239, 384, 481, 746
BstMWI GCNNNNNNNGC 6 cut(s) 406, 429, 510, 597, 860, 869
BstNI CCWGG 2 cut(s) 231, 247
BstNSI RCATGY 1 cut(s) 824
BstSCI CCNGG 3 cut(s) 204, 229, 245
BstSFI CTRYAG 1 cut(s) 279
BstV1I GCAGC 3 cut(s) 169, 584, 847
BstX2I RGATCY 2 cut(s) 239, 384
BstYI RGATCY 2 cut(s) 239, 384
BsuRI GGCC 1 cut(s) 304
BtrI CACGTC 1 cut(s) 561
BtsCI GGATG 1 cut(s) 697
BtsI GCAGTG 1 cut(s) 805
BtsIMutI CAGTG 3 cut(s) 624, 765, 805
Cac8I GCNNGC 2 cut(s) 541, 818
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 1 cut(s) 252
Cfr13I GGNCC 2 cut(s) 103, 154
Csp6I GTAC 2 cut(s) 88, 909
CviAII CATG 2 cut(s) 401, 821
CviQI GTAC 2 cut(s) 88, 909
DdeI CTNAG 3 cut(s) 11, 899, 917
DpnI GATC 5 cut(s) 113, 241, 386, 483, 748
DpnII GATC 5 cut(s) 111, 239, 384, 481, 746
Eam1104I CTCTTC 4 cut(s) 259, 260, 293, 723
EarI CTCTTC 4 cut(s) 259, 260, 293, 723
EciI GGCGGA 2 cut(s) 39, 63
Eco147I AGGCCT 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 758
Eco47I GGWCC 2 cut(s) 103, 154
Eco57I CTGAAG 4 cut(s) 66, 185, 741, 771
EcoRII CCWGG 2 cut(s) 229, 245
EcoT38I GRGCYC 1 cut(s) 335
FaeI CATG 2 cut(s) 404, 824
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 3 cut(s) 91, 116, 183
FatI CATG 2 cut(s) 400, 820
FauI CCCGC 1 cut(s) 914
Fnu4HI GCNGC 4 cut(s) 183, 186, 598, 861
FokI GGATG 1 cut(s) 684
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 4 cut(s) 183, 186, 598, 861
FspBI CTAG 2 cut(s) 258, 813
GlaI GCGC 1 cut(s) 251
GluI GCNGC 4 cut(s) 183, 186, 598, 861
GsaI CCCAGC 2 cut(s) 146, 436
HaeII RGCGCY 1 cut(s) 253
HaeIII GGCC 1 cut(s) 304
HapII CCGG 3 cut(s) 205, 237, 485
HhaI GCGC 1 cut(s) 252
Hin1II CATG 2 cut(s) 404, 824
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 1 cut(s) 782
HpaII CCGG 3 cut(s) 205, 237, 485
HphI GGTGA 2 cut(s) 737, 839
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 3 cut(s) 12, 116, 808
Hpy188III TCNNGA 4 cut(s) 172, 237, 551, 789
Hpy8I GTNNAC 1 cut(s) 877
HpyAV CCTTC 4 cut(s) 362, 604, 765, 897
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 2 cut(s) 196, 560
HpyCH4V TGCA 6 cut(s) 136, 292, 513, 591, 740, 848
HpyF10VI GCNNNNNNNGC 6 cut(s) 406, 429, 510, 597, 860, 869
HpyF3I CTNAG 3 cut(s) 11, 899, 917
HpySE526I ACGT 2 cut(s) 196, 560
Hsp92II CATG 2 cut(s) 404, 824
HspAI GCGC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 236
Kzo9I GATC 5 cut(s) 111, 239, 384, 481, 746
LguI GCTCTTC 1 cut(s) 259
LmnI GCTCC 3 cut(s) 50, 280, 397
Lsp1109I GCAGC 3 cut(s) 169, 584, 847
LweI GCATC 2 cut(s) 588, 841
MaeI CTAG 2 cut(s) 258, 813
MaeII ACGT 2 cut(s) 196, 560
MaeIII GTNAC 3 cut(s) 584, 659, 827
MalI GATC 5 cut(s) 113, 241, 386, 483, 748
MbiI CCGCTC 1 cut(s) 921
MboI GATC 5 cut(s) 111, 239, 384, 481, 746
MboII GAAGA 6 cut(s) 246, 277, 310, 473, 740, 777
MfeI CAATTG 1 cut(s) 592
MflI RGATCY 2 cut(s) 239, 384
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 5 cut(s) 354, 497, 592, 792, 925
MroI TCCGGA 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 2 cut(s) 489, 928
MslI CAYNNNNRTG 1 cut(s) 825
MspI CCGG 3 cut(s) 205, 237, 485
MspR9I CCNGG 3 cut(s) 206, 231, 247
MunI CAATTG 1 cut(s) 592
MvaI CCWGG 2 cut(s) 231, 247
MwoI GCNNNNNNNGC 6 cut(s) 406, 429, 510, 597, 860, 869
NciI CCSGG 1 cut(s) 206
NdeII GATC 5 cut(s) 111, 239, 384, 481, 746
NlaIII CATG 2 cut(s) 404, 824
NlaIV GGNNCC 5 cut(s) 104, 235, 241, 276, 621
NmuCI GTSAC 1 cut(s) 827
NspI RCATGY 1 cut(s) 824
PceI AGGCCT 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 1 cut(s) 782
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 4 cut(s) 184, 187, 599, 862
PsiI TTATAA 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 229, 245
PspFI CCCAGC 2 cut(s) 142, 432
PspGI CCWGG 2 cut(s) 229, 245
PspN4I GGNNCC 5 cut(s) 104, 235, 241, 276, 621
PspPI GGNCC 2 cut(s) 103, 154
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 2 cut(s) 239, 384
RsaI GTAC 2 cut(s) 89, 910
RsaNI GTAC 2 cut(s) 88, 909
RseI CAYNNNNRTG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 259
SaqAI TTAA 2 cut(s) 489, 928
SatI GCNGC 4 cut(s) 183, 186, 598, 861
Sau3AI GATC 5 cut(s) 111, 239, 384, 481, 746
Sau96I GGNCC 2 cut(s) 103, 154
ScrFI CCNGG 3 cut(s) 206, 231, 247
SduI GDGCHC 1 cut(s) 335
SfaNI GCATC 2 cut(s) 588, 841
SfcI CTRYAG 1 cut(s) 279
SinI GGWCC 2 cut(s) 103, 154
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 5 cut(s) 354, 497, 592, 792, 925
SseBI AGGCCT 1 cut(s) 304
SsiI CCGC 8 cut(s) 5, 24, 36, 48, 72, 186, 694, 921
SspMI CTAG 2 cut(s) 258, 813
StuI AGGCCT 1 cut(s) 304
StyD4I CCNGG 3 cut(s) 204, 229, 245
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 2 cut(s) 199, 563
TaqI TCGA 1 cut(s) 19
TaqII GACCGA 1 cut(s) 191
TasI AATT 5 cut(s) 354, 497, 592, 792, 925
TatI WGTACW 1 cut(s) 908
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 1 cut(s) 782
Tru1I TTAA 2 cut(s) 489, 928
Tru9I TTAA 2 cut(s) 489, 928
TscAI CASTG 3 cut(s) 631, 765, 805
TseFI GTSAC 1 cut(s) 827
TseI GCWGC 3 cut(s) 182, 597, 860
Tsp45I GTSAC 1 cut(s) 827
TspDTI ATGAA 2 cut(s) 323, 795
TspRI CASTG 3 cut(s) 631, 765, 805
Van91I CCANNNNNTGG 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 103, 154
XapI RAATTY 2 cut(s) 354, 497
XceI RCATGY 1 cut(s) 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 2 cut(s) 258, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.