Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001417

Physical Location & Seq

Genomic Coordinates Forward (+)

25807631 .. 25811906

Gene Structure 4276 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1221400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 915 bp

ATGTATAGGGAACGTGGCGGTGGAGGCGTTGGCGGGTCATCGAAAACAGAGATTGTTGGTGGGTCGCTGGACCGCAAGCGCATCAACGATGCGCTGGAACGCAGGCGCATCAACGATGCGCTGGACAAGCACCTAGTGAATTCCTCGACTTCCACATCGAGAGCTTTGGCTAACAGTAAAGACAAAGAGAGGCTCTCTGTGCCCTCCACTTCCGCTGGTAAATCTCAACTCGATCACCGTGCTGCTGCCGCCGCGGCCGCGTCGCTCGCTGAGAACAAATGCTCTGATGAGGAATCTGAAACAGACAGTGAAGAATCAGATGTCAGTGGTTCTGATGGAGATGACACATCTTGGATTTCATGGTTCTGCAATTTGCGAGGAAATGAATTTTTTTGTGAAGTGGATGATGAATATATTCAAGATGATTTTAATCTCTGCGGTTTGAGCAGCCAAGTTCCATATTATGATTATGCACTTGATCTTATTTTGGATGTTGAATCTTCTCATGGTGACATGTTCACTGAAGAGCAGAATGAGTTGGTTGAATCAGCAGCGGAGATGCTATACGGTCTTATACATGTTCGATACATACTGACTAGCAAAGGAATGTCTGCAATGTTGGAGAAATACAAGAGCTATGACTTTGGAAGATGCCCAAGAGTTTACTGCTGTGGACAACCCTGCCTTCCAGTTGGTCAATCGGACATTCCTCGTTCAAGCACTGTAAAAATATACTGCCCCAAATGCGAAGATATATATTACCCTCGATCGAAGTACCAAGGCAATATTGATGGAGCATATTTTGGAACCACATTTCCGCACTTGTTCTTGATGACATATGGGCACCTTAAGCCACAAAAGGCGTCACAGAGTTACGTGCCAAGAGTTTTTGGTTTCAAGCTCCACAAGCCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009987 GO:0010228 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0032501 GO:0032502 GO:0036211 GO:0042752 GO:0042753 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048511 GO:0048518 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0061458 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03008 ko04064 ko04137 ko04139 ko04310 ko04520 ko04712 ko05162 ko05168 ko05169 map03008 map04064 map04137 map04139 map04310 map04520 map04712 map05162 map05168 map05169

Pfam Domains

Protein Families

CK_II_beta

Protein Analysis

305

Amino Acids

33.87

Weight (kDa)

5.18

Isoelectric Point (pI)

47.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CK_II_beta	PF01214	117 - 300	3.3e-77	Casein kinase II regulatory subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001417)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44680 AT2G44680 AT2G44680 AT3G60250 AT3G60250 AT3G60250
fragaria_vesca	FvH4_5g20700 FvH4_5g20700 FvH4_5g20700 FvH4_5g20700 FvH4_5g20700 FvH4_5g20700 FvH4_7g09090
malus_domestica	MD02G1221400.v1.1 MD06G1044800.v1.1 MD07G1094900.v1.1
prunus_persica	Prupe.2G119400_v2.0.a1 Prupe.2G119400_v2.0.a1 Prupe.5G053000_v2.0.a1 Prupe.5G053000_v2.0.a1 Prupe.5G053000_v2.0.a1 Prupe.5G053000_v2.0.a1
pyrus_communis	pycom02g18690 pycom04g04720 pycom06g03810 pycom07g07820
rosa_chinensis	RchiOBHm_Chr1g0344941 RchiOBHm_Chr7g0206431
rosa_laevigata	RLG00000003312 RLG00000028865
rosa_multiflora	Rmu_sc0000390.1_g000005 Rmu_sc0003179.1_g000030 Rmu_sc0003652.1_g000022 Rmu_sc0005065.1_g000027 Rmu_sc0010057.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251510 Rroxscaffold_4G00309500
rosa_rugosa	Rorug01G0175200 Rorug07G0097500.1 Rorug07G0097600 Rorug07G0097600 Rorug07G0097700 Rorug07G0097700
rosa_samantha	Rh4DG119000 Rh7AG229200 Rh7BG225600 Rh7CG243700 Rh7DG236100
rosa_wichuraiana	Rw1G008730 Rw1G015900 Rw2G005570 Rw7G019720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	843
AccII	CGCG	2 cut(s)	254, 260
AcoI	YGGCCR	1 cut(s)	255
AcsI	RAATTY	2 cut(s)	139, 386
AcuI	CTGAAG	1 cut(s)	543
AcyI	GRCGYC	1 cut(s)	863
AdeI	CACNNNGTG	1 cut(s)	136
AfaI	GTAC	1 cut(s)	776
AflII	CTTAAG	1 cut(s)	848
AflIII	ACRYGT	2 cut(s)	513, 577
AgsI	TTSAA	5 cut(s)	419, 497, 545, 717, 898
AloI	GAACNNNNNNTCC	2 cut(s)	799, 831
AluBI	AGCT	3 cut(s)	164, 636, 901
AluI	AGCT	3 cut(s)	164, 636, 901
AoxI	GGCC	1 cut(s)	255
ApeKI	GCWGC	4 cut(s)	242, 245, 447, 551
ApoI	RAATTY	2 cut(s)	139, 386
Asp700I	GAANNNNTTC	1 cut(s)	414
AspLEI	GCGC	4 cut(s)	81, 94, 108, 121
AspS9I	GGNCC	1 cut(s)	70
AsuHPI	GGTGA	2 cut(s)	227, 521
AvaII	GGWCC	1 cut(s)	70
BaeGI	GKGCMC	2 cut(s)	204, 846
BanI	GGYRCC	1 cut(s)	843
BbvI	GCAGC	4 cut(s)	229, 232, 459, 563
BccI	CCATC	2 cut(s)	329, 785
BceAI	ACGGC	1 cut(s)	895
BcgI	CGANNNNNNTGC	2 cut(s)	221, 255
BfaI	CTAG	2 cut(s)	134, 597
BfrI	CTTAAG	1 cut(s)	848
BglI	GCCNNNNNGGC	1 cut(s)	254
BisI	GCNGC	8 cut(s)	243, 246, 249, 252, 255, 258, 448, 552
BlsI	GCNGC	8 cut(s)	244, 247, 250, 253, 256, 259, 449, 553
Bme18I	GGWCC	1 cut(s)	70
BmgT120I	GGNCC	1 cut(s)	70
BmiI	GGNNCC	2 cut(s)	808, 845
BmsI	GCATC	6 cut(s)	79, 90, 106, 117, 549, 641
BplI	GAGNNNNNCTC	2 cut(s)	179, 211
BsaAI	YACGTR	1 cut(s)	877
BsaBI	GATNNNNATC	1 cut(s)	429
BsaHI	GRCGYC	1 cut(s)	863
BsaJI	CCNNGG	2 cut(s)	252, 778
Bse1I	ACTGG	1 cut(s)	689
Bse3DI	GCAATG	1 cut(s)	621
Bse8I	GATNNNNATC	1 cut(s)	429
BseDI	CCNNGG	2 cut(s)	252, 778
BseGI	GGATG	2 cut(s)	409, 496
BseJI	GATNNNNATC	1 cut(s)	429
BseMI	GCAATG	1 cut(s)	621
BseMII	CTCAG	1 cut(s)	261
BseNI	ACTGG	1 cut(s)	689
BseSI	GKGCMC	2 cut(s)	204, 846
BseX3I	CGGCCG	1 cut(s)	255
BseXI	GCAGC	4 cut(s)	229, 232, 459, 563
Bsh1236I	CGCG	2 cut(s)	254, 260
Bsh1285I	CGRYCG	2 cut(s)	258, 770
BshFI	GGCC	1 cut(s)	257
BshNI	GGYRCC	1 cut(s)	843
BsiEI	CGRYCG	2 cut(s)	258, 770
BsnI	GGCC	1 cut(s)	257
Bsp1286I	GDGCHC	2 cut(s)	204, 846
Bsp143I	GATC	3 cut(s)	232, 478, 767
BspANI	GGCC	1 cut(s)	257
BspCNI	CTCAG	1 cut(s)	262
BspFNI	CGCG	2 cut(s)	254, 260
BspLI	GGNNCC	2 cut(s)	808, 845
BspQI	GCTCTTC	1 cut(s)	519
BspT107I	GGYRCC	1 cut(s)	843
BspTI	CTTAAG	1 cut(s)	848
BsrDI	GCAATG	1 cut(s)	621
BsrI	ACTGG	1 cut(s)	689
BssECI	CCNNGG	2 cut(s)	252, 778
BssMI	GATC	3 cut(s)	232, 478, 767
BssNI	GRCGYC	1 cut(s)	863
BssT1I	CCWWGG	1 cut(s)	778
Bst4CI	ACNGT	5 cut(s)	176, 239, 308, 569, 724
Bst6I	CTCTTC	1 cut(s)	519
BstACI	GRCGYC	1 cut(s)	863
BstAFI	CTTAAG	1 cut(s)	848
BstBAI	YACGTR	1 cut(s)	877
BstC8I	GCNNGC	3 cut(s)	77, 104, 267
BstDEI	CTNAG	1 cut(s)	270
BstDSI	CCRYGG	1 cut(s)	252
BstF5I	GGATG	2 cut(s)	409, 496
BstFNI	CGCG	2 cut(s)	254, 260
BstHHI	GCGC	4 cut(s)	81, 94, 108, 121
BstKTI	GATC	3 cut(s)	235, 481, 770
BstMBI	GATC	3 cut(s)	232, 478, 767
BstMCI	CGRYCG	2 cut(s)	258, 770
BstNSI	RCATGY	2 cut(s)	517, 581
BstSLI	GKGCMC	2 cut(s)	204, 846
BstUI	CGCG	2 cut(s)	254, 260
BstV1I	GCAGC	4 cut(s)	229, 232, 459, 563
BstZI	CGGCCG	1 cut(s)	255
BsuRI	GGCC	1 cut(s)	257
BtgI	CCRYGG	1 cut(s)	252
BtsCI	GGATG	2 cut(s)	409, 496
BtsIMutI	CAGTG	4 cut(s)	313, 331, 519, 720
Cac8I	GCNNGC	3 cut(s)	77, 104, 267
CciNI	GCGGCCGC	1 cut(s)	255
CfoI	GCGC	4 cut(s)	81, 94, 108, 121
Cfr13I	GGNCC	1 cut(s)	70
Cfr42I	CCGCGG	1 cut(s)	255
CseI	GACGC	2 cut(s)	249, 852
Csp6I	GTAC	1 cut(s)	775
CviAII	CATG	4 cut(s)	360, 506, 514, 578
CviJI	RGCY	9 cut(s)	164, 170, 193, 257, 450, 636, 853, 901, 910
CviKI_1	RGCY	9 cut(s)	164, 170, 193, 257, 450, 636, 853, 901, 910
CviQI	GTAC	1 cut(s)	775
DdeI	CTNAG	1 cut(s)	270
DpnI	GATC	3 cut(s)	234, 480, 769
DpnII	GATC	3 cut(s)	232, 478, 767
DraIII	CACNNNGTG	1 cut(s)	136
EaeI	YGGCCR	1 cut(s)	255
EagI	CGGCCG	1 cut(s)	255
Eam1104I	CTCTTC	1 cut(s)	519
EarI	CTCTTC	1 cut(s)	519
EclXI	CGGCCG	1 cut(s)	255
Eco130I	CCWWGG	1 cut(s)	778
Eco47I	GGWCC	1 cut(s)	70
Eco52I	CGGCCG	1 cut(s)	255
Eco57I	CTGAAG	1 cut(s)	543
EcoRI	GAATTC	1 cut(s)	139
EcoT14I	CCWWGG	1 cut(s)	778
ErhI	CCWWGG	1 cut(s)	778
FaeI	CATG	4 cut(s)	363, 509, 517, 581
FatI	CATG	4 cut(s)	359, 505, 513, 577
FauI	CCCGC	1 cut(s)	26
FauNDI	CATATG	1 cut(s)	838
Fnu4HI	GCNGC	8 cut(s)	243, 246, 249, 252, 255, 258, 448, 552
FokI	GGATG	2 cut(s)	416, 503
Fsp4HI	GCNGC	8 cut(s)	243, 246, 249, 252, 255, 258, 448, 552
FspBI	CTAG	2 cut(s)	134, 597
GlaI	GCGC	4 cut(s)	80, 93, 107, 120
GluI	GCNGC	8 cut(s)	243, 246, 249, 252, 255, 258, 448, 552
HaeIII	GGCC	1 cut(s)	257
HgaI	GACGC	2 cut(s)	249, 852
HhaI	GCGC	4 cut(s)	81, 94, 108, 121
Hin1I	GRCGYC	1 cut(s)	863
Hin1II	CATG	4 cut(s)	363, 509, 517, 581
Hin6I	GCGC	4 cut(s)	79, 92, 106, 119
HinP1I	GCGC	4 cut(s)	79, 92, 106, 119
HinfI	GANTC	4 cut(s)	293, 314, 497, 545
HphI	GGTGA	2 cut(s)	227, 521
Hpy166II	GTNNAC	3 cut(s)	519, 664, 674
Hpy188I	TCNGA	5 cut(s)	286, 298, 319, 334, 703
Hpy188III	TCNNGA	3 cut(s)	159, 419, 829
Hpy8I	GTNNAC	3 cut(s)	519, 664, 674
Hpy99I	CGWCG	1 cut(s)	265
HpyAV	CCTTC	1 cut(s)	695
HpyCH4III	ACNGT	5 cut(s)	176, 239, 308, 569, 724
HpyCH4IV	ACGT	2 cut(s)	13, 876
HpyCH4V	TGCA	3 cut(s)	369, 473, 614
HpyF3I	CTNAG	1 cut(s)	270
HpySE526I	ACGT	2 cut(s)	13, 876
Hsp92I	GRCGYC	1 cut(s)	863
Hsp92II	CATG	4 cut(s)	363, 509, 517, 581
HspAI	GCGC	4 cut(s)	79, 92, 106, 119
KspI	CCGCGG	1 cut(s)	255
Kzo9I	GATC	3 cut(s)	232, 478, 767
LguI	GCTCTTC	1 cut(s)	519
LmnI	GCTCC	2 cut(s)	794, 906
LpnPI	CCDG	7 cut(s)	53, 80, 88, 107, 201, 694, 702
Lsp1109I	GCAGC	4 cut(s)	229, 232, 459, 563
LweI	GCATC	6 cut(s)	79, 90, 106, 117, 549, 641
MaeI	CTAG	2 cut(s)	134, 597
MaeII	ACGT	2 cut(s)	13, 876
MaeIII	GTNAC	3 cut(s)	509, 864, 872
MalI	GATC	3 cut(s)	234, 480, 769
MboI	GATC	3 cut(s)	232, 478, 767
MboII	GAAGA	5 cut(s)	323, 492, 536, 660, 761
MhlI	GDGCHC	2 cut(s)	204, 846
MluCI	AATT	3 cut(s)	139, 370, 386
MmeI	TCCRAC	1 cut(s)	600
MnlI	CCTC	8 cut(s)	17, 154, 183, 214, 283, 371, 720, 774
MroXI	GAANNNNTTC	1 cut(s)	414
MseI	TTAA	2 cut(s)	429, 849
MspA1I	CMGCKG	3 cut(s)	215, 254, 554
MspCI	CTTAAG	1 cut(s)	848
MvnI	CGCG	2 cut(s)	254, 260
NdeI	CATATG	1 cut(s)	838
NdeII	GATC	3 cut(s)	232, 478, 767
NlaIII	CATG	4 cut(s)	363, 509, 517, 581
NlaIV	GGNNCC	2 cut(s)	808, 845
NmuCI	GTSAC	2 cut(s)	509, 864
NotI	GCGGCCGC	1 cut(s)	255
NspI	RCATGY	2 cut(s)	517, 581
PciI	ACATGT	2 cut(s)	513, 577
PciSI	GCTCTTC	1 cut(s)	519
PdmI	GAANNNNTTC	1 cut(s)	414
PfeI	GAWTC	4 cut(s)	293, 314, 497, 545
PkrI	GCNGC	8 cut(s)	244, 247, 250, 253, 256, 259, 449, 553
Ple19I	CGATCG	1 cut(s)	770
Ppu21I	YACGTR	1 cut(s)	877
PscI	ACATGT	2 cut(s)	513, 577
PspN4I	GGNNCC	2 cut(s)	808, 845
PspPI	GGNCC	1 cut(s)	70
PvuI	CGATCG	1 cut(s)	770
RsaI	GTAC	1 cut(s)	776
RsaNI	GTAC	1 cut(s)	775
SacII	CCGCGG	1 cut(s)	255
SapI	GCTCTTC	1 cut(s)	519
SaqAI	TTAA	2 cut(s)	429, 849
SatI	GCNGC	8 cut(s)	243, 246, 249, 252, 255, 258, 448, 552
Sau3AI	GATC	3 cut(s)	232, 478, 767
Sau96I	GGNCC	1 cut(s)	70
SduI	GDGCHC	2 cut(s)	204, 846
SetI	ASST	7 cut(s)	16, 135, 166, 638, 849, 879, 903
SfaNI	GCATC	6 cut(s)	79, 90, 106, 117, 549, 641
Sfr303I	CCGCGG	1 cut(s)	255
SgrBI	CCGCGG	1 cut(s)	255
SinI	GGWCC	1 cut(s)	70
SmlI	CTYRAG	1 cut(s)	848
SmoI	CTYRAG	1 cut(s)	848
Sse9I	AATT	3 cut(s)	139, 370, 386
SspI	AATATT	1 cut(s)	787
SspMI	CTAG	2 cut(s)	134, 597
StyI	CCWWGG	1 cut(s)	778
TaaI	ACNGT	5 cut(s)	176, 239, 308, 569, 724
TaiI	ACGT	2 cut(s)	16, 879
TaqI	TCGA	7 cut(s)	41, 146, 158, 231, 583, 766, 770
TasI	AATT	3 cut(s)	139, 370, 386
TauI	GCSGC	4 cut(s)	251, 254, 257, 260
TfiI	GAWTC	4 cut(s)	293, 314, 497, 545
Tru1I	TTAA	2 cut(s)	429, 849
Tru9I	TTAA	2 cut(s)	429, 849
TscAI	CASTG	4 cut(s)	313, 331, 526, 727
TseFI	GTSAC	2 cut(s)	509, 864
TseI	GCWGC	4 cut(s)	242, 245, 447, 551
Tsp45I	GTSAC	2 cut(s)	509, 864
TspDTI	ATGAA	3 cut(s)	348, 399, 423
TspRI	CASTG	4 cut(s)	313, 331, 526, 727
Vha464I	CTTAAG	1 cut(s)	848
VpaK11BI	GGWCC	1 cut(s)	70
XapI	RAATTY	2 cut(s)	139, 386
XceI	RCATGY	2 cut(s)	517, 581
XmnI	GAANNNNTTC	1 cut(s)	414
XspI	CTAG	2 cut(s)	134, 597

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.