MD06G1044800.v1.1

Plays a complex role in regulating the basal catalytic activity of the alpha subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001417
Physical Location & Seq
Reverse (-)
6117538 .. 6121302
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1044800.v1.1.491

Sequence Viewer

Length: 789 bp
ATGCACAGAGAGCGAGGGTTGGGTGGGTCTAAGGCGACGGAGGTGGTGGGACCCGCCGCTGATCGGAAGCAGCGGATCAACGACGCCTTGGACAAGCAGCTCGAGCGATCCTCGCCCTCCACTTCTAGGGCAATCAACGGCAAGGATAAGCCGCTTATTATGGGTGAAGAATCTGAGACAGACAGTGAAGAGTCAGATGTTAGTGGTTCCGATGGGGATGACACATCTTGGATTTCATGGTTTTGCAATTTGCGAGGAAATGAATTTTTCTGTGAAGTGGATGATGATTACATACAAGATGATTTTAACCTCTGCGGGTTAAGCAGCCAAGTGCCGTACTATGATTATGCACTTGATTTAATTTTGGATGTTGAATCTTCTCATGGTGATATGTTCACAGAAGAACAAAATGAATTGATTGAATCAGCAGCAGAGATGCTTTACGGTCTTATTCATGTTCGATATATATTGACAAGCAAAGGCTTGGCTGCCATGCTAGACAAGTACAAGAACTATGATTTTGGTAGATGCCCAAGAGTTTTCTGCTGCGGACAACCCTGCCTCCCAGTTGGCCAGTCGGATATCCCACGGTCAAGCACTGTAAAAATATACTGCCCCAAGTGTGAAGATATTTACTACCCTCGATCGAAGTATCAAGACATTGATGGAGCCTATTTCGGAAGTACATTCCCTCACCTGTTCTTGATGACATACGGGCACCTGAAGCCTCAGAAAGCATCACAGAGTTATGTTCCTAGAGTGTTCGGATTCAAGCTTCACAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko04064 ko04137 ko04139 ko04310 ko04520 ko04712 ko05162 ko05168 ko05169 map03008 map04064 map04137 map04139 map04310 map04520 map04712 map05162 map05168 map05169
Pfam Domains
Protein Families
CK_II_beta

Protein Analysis

263

Amino Acids

29.61

Weight (kDa)

4.9

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CK_II_beta PF01214 76 - 258 3.9e-76 Casein kinase II regulatory subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 717
AciI CCGC 6 cut(s) 54, 57, 73, 152, 315, 549
AclWI GGATC 2 cut(s) 83, 102
AcoI YGGCCR 1 cut(s) 571
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 1 cut(s) 743
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 3 cut(s) 338, 506, 685
AfiI CCNNNNNNNGG 2 cut(s) 63, 126
AgsI TTSAA 3 cut(s) 374, 422, 772
AluBI AGCT 2 cut(s) 100, 775
AluI AGCT 2 cut(s) 100, 775
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 2 cut(s) 83, 102
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 1 cut(s) 571
ApeKI GCWGC 6 cut(s) 70, 97, 324, 428, 488, 546
ApoI RAATTY 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 3 cut(s) 176, 398, 686
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 1 cut(s) 50
BaeGI GKGCMC 1 cut(s) 720
BalI TGGCCA 1 cut(s) 573
BanI GGYRCC 1 cut(s) 717
BbvI GCAGC 6 cut(s) 82, 109, 336, 440, 475, 533
BccI CCATC 2 cut(s) 206, 659
BceAI ACGGC 2 cut(s) 154, 319
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 3 cut(s) 126, 497, 756
BisI GCNGC 8 cut(s) 57, 71, 98, 152, 325, 429, 489, 547
BlsI GCNGC 8 cut(s) 58, 72, 99, 153, 326, 430, 490, 548
Bme18I GGWCC 1 cut(s) 50
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 5 cut(s) 51, 52, 208, 670, 719
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 3 cut(s) 426, 518, 746
BmuI ACTGGG 1 cut(s) 560
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 87, 587
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 126
Bse1I ACTGG 2 cut(s) 566, 574
BseDI CCNNGG 2 cut(s) 87, 587
BseGI GGATG 3 cut(s) 223, 286, 373
BseLI CCNNNNNNNGG 2 cut(s) 63, 126
BseMII CTCAG 2 cut(s) 165, 743
BseNI ACTGG 2 cut(s) 566, 574
BseSI GKGCMC 1 cut(s) 720
BseXI GCAGC 6 cut(s) 82, 109, 336, 440, 475, 533
Bsh1285I CGRYCG 1 cut(s) 647
BshFI GGCC 1 cut(s) 573
BshNI GGYRCC 1 cut(s) 717
BsiEI CGRYCG 1 cut(s) 647
BsiHKCI CYCGRG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 63, 126
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 1 cut(s) 573
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 720
Bsp143I GATC 4 cut(s) 61, 75, 107, 644
BspACI CCGC 6 cut(s) 54, 57, 73, 152, 315, 549
BspANI GGCC 1 cut(s) 573
BspCNI CTCAG 2 cut(s) 166, 742
BspLI GGNNCC 5 cut(s) 51, 52, 208, 670, 719
BspPI GGATC 2 cut(s) 83, 102
BspT107I GGYRCC 1 cut(s) 717
BsrI ACTGG 2 cut(s) 566, 574
BssECI CCNNGG 2 cut(s) 87, 587
BssMI GATC 4 cut(s) 61, 75, 107, 644
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 4 cut(s) 185, 446, 591, 601
Bst6I CTCTTC 1 cut(s) 183
BstACI GRCGYC 1 cut(s) 84
BstDEI CTNAG 3 cut(s) 30, 174, 729
BstDSI CCRYGG 1 cut(s) 587
BstF5I GGATG 3 cut(s) 223, 286, 373
BstKTI GATC 4 cut(s) 64, 78, 110, 647
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 4 cut(s) 61, 75, 107, 644
BstMCI CGRYCG 1 cut(s) 647
BstMWI GCNNNNNNNGC 6 cut(s) 10, 103, 112, 321, 724, 781
BstSLI GKGCMC 1 cut(s) 720
BstV1I GCAGC 6 cut(s) 82, 109, 336, 440, 475, 533
BsuRI GGCC 1 cut(s) 573
BtgI CCRYGG 1 cut(s) 587
BtsCI GGATG 3 cut(s) 223, 286, 373
BtsIMutI CAGTG 2 cut(s) 190, 597
Cfr13I GGNCC 1 cut(s) 50
CseI GACGC 1 cut(s) 92
Csp6I GTAC 3 cut(s) 337, 505, 684
CviAII CATG 5 cut(s) 237, 383, 455, 493, 786
CviQI GTAC 3 cut(s) 337, 505, 684
DdeI CTNAG 3 cut(s) 30, 174, 729
DpnI GATC 4 cut(s) 63, 77, 109, 646
DpnII GATC 4 cut(s) 61, 75, 107, 644
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 87
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 50
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 240, 386, 458, 496, 789
FaqI GGGAC 1 cut(s) 63
FatI CATG 5 cut(s) 236, 382, 454, 492, 785
FauI CCCGC 2 cut(s) 61, 308
Fnu4HI GCNGC 8 cut(s) 57, 71, 98, 152, 325, 429, 489, 547
FokI GGATG 3 cut(s) 230, 293, 380
Fsp4HI GCNGC 8 cut(s) 57, 71, 98, 152, 325, 429, 489, 547
FspBI CTAG 3 cut(s) 126, 497, 756
GluI GCNGC 8 cut(s) 57, 71, 98, 152, 325, 429, 489, 547
HaeIII GGCC 1 cut(s) 573
HgaI GACGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 5 cut(s) 240, 386, 458, 496, 789
HindIII AAGCTT 1 cut(s) 773
HinfI GANTC 5 cut(s) 170, 191, 374, 422, 768
HphI GGTGA 3 cut(s) 176, 398, 686
Hpy166II GTNNAC 1 cut(s) 396
Hpy188I TCNGA 8 cut(s) 66, 175, 196, 211, 580, 680, 732, 767
Hpy188III TCNNGA 2 cut(s) 656, 703
Hpy8I GTNNAC 1 cut(s) 396
Hpy99I CGWCG 2 cut(s) 40, 86
HpyCH4III ACNGT 4 cut(s) 185, 446, 591, 601
HpyCH4V TGCA 3 cut(s) 4, 246, 350
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 103, 112, 321, 724, 781
HpyF3I CTNAG 3 cut(s) 30, 174, 729
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 5 cut(s) 240, 386, 458, 496, 789
KflI GGGWCCC 1 cut(s) 50
Kzo9I GATC 4 cut(s) 61, 75, 107, 644
LmnI GCTCC 1 cut(s) 668
LpnPI CCDG 5 cut(s) 571, 579, 587, 710, 734
Lsp1109I GCAGC 6 cut(s) 82, 109, 336, 440, 475, 533
LweI GCATC 3 cut(s) 426, 518, 746
MaeI CTAG 3 cut(s) 126, 497, 756
MalI GATC 4 cut(s) 63, 77, 109, 646
MboI GATC 4 cut(s) 61, 75, 107, 644
MboII GAAGA 5 cut(s) 179, 200, 369, 413, 638
MhlI GDGCHC 1 cut(s) 720
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 4 cut(s) 247, 263, 360, 413
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 558
Mox20I TGGCCA 1 cut(s) 573
MscI TGGCCA 1 cut(s) 573
MseI TTAA 3 cut(s) 306, 320, 359
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 2 cut(s) 59, 73
MwoI GCNNNNNNNGC 6 cut(s) 10, 103, 112, 321, 724, 781
NdeII GATC 4 cut(s) 61, 75, 107, 644
NlaIII CATG 5 cut(s) 240, 386, 458, 496, 789
NlaIV GGNNCC 5 cut(s) 51, 52, 208, 670, 719
PaeR7I CTCGAG 1 cut(s) 101
PfeI GAWTC 4 cut(s) 170, 374, 422, 768
PkrI GCNGC 8 cut(s) 58, 72, 99, 153, 326, 430, 490, 548
Ple19I CGATCG 1 cut(s) 647
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PpuMI RGGWCCY 1 cut(s) 50
Psp5II RGGWCCY 1 cut(s) 50
PspN4I GGNNCC 5 cut(s) 51, 52, 208, 670, 719
PspPI GGNCC 1 cut(s) 50
PspPPI RGGWCCY 1 cut(s) 50
PspXI VCTCGAGB 1 cut(s) 101
PvuI CGATCG 1 cut(s) 647
RsaI GTAC 3 cut(s) 338, 506, 685
RsaNI GTAC 3 cut(s) 337, 505, 684
SaqAI TTAA 3 cut(s) 306, 320, 359
SatI GCNGC 8 cut(s) 57, 71, 98, 152, 325, 429, 489, 547
Sau3AI GATC 4 cut(s) 61, 75, 107, 644
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 1 cut(s) 200
SduI GDGCHC 1 cut(s) 720
SetI ASST 6 cut(s) 45, 102, 312, 699, 723, 777
SfaNI GCATC 3 cut(s) 426, 518, 746
Sfr274I CTCGAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 50
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 4 cut(s) 247, 263, 360, 413
SsiI CCGC 6 cut(s) 54, 57, 73, 152, 315, 549
SspMI CTAG 3 cut(s) 126, 497, 756
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 4 cut(s) 185, 446, 591, 601
TaqI TCGA 4 cut(s) 102, 460, 643, 647
TasI AATT 4 cut(s) 247, 263, 360, 413
TatI WGTACW 2 cut(s) 504, 683
TauI GCSGC 2 cut(s) 59, 154
TfiI GAWTC 4 cut(s) 170, 374, 422, 768
Tru1I TTAA 3 cut(s) 306, 320, 359
Tru9I TTAA 3 cut(s) 306, 320, 359
TscAI CASTG 2 cut(s) 190, 604
TseI GCWGC 6 cut(s) 70, 97, 324, 428, 488, 546
TspDTI ATGAA 4 cut(s) 225, 276, 426, 443
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 2 cut(s) 190, 604
VpaK11BI GGWCC 1 cut(s) 50
XapI RAATTY 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 101
XspI CTAG 3 cut(s) 126, 497, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.