Plays a complex role in regulating the basal catalytic activity of the alpha subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 12 |
| AciI | CCGC | 1 cut(s) | 278 |
| AcoI | YGGCCR | 1 cut(s) | 418 |
| AdeI | CACNNNGTG | 1 cut(s) | 233 |
| AfaI | GTAC | 2 cut(s) | 306, 389 |
| AgsI | TTSAA | 2 cut(s) | 154, 269 |
| AoxI | GGCC | 1 cut(s) | 418 |
| ApeKI | GCWGC | 3 cut(s) | 171, 275, 335 |
| AseI | ATTAAT | 1 cut(s) | 263 |
| AspLEI | GCGC | 1 cut(s) | 12 |
| AsuHPI | GGTGA | 1 cut(s) | 245 |
| BalI | TGGCCA | 1 cut(s) | 420 |
| BbsI | GAAGAC | 1 cut(s) | 216 |
| BbvI | GCAGC | 3 cut(s) | 183, 287, 322 |
| BccI | CCATC | 1 cut(s) | 53 |
| BfaI | CTAG | 1 cut(s) | 344 |
| BisI | GCNGC | 3 cut(s) | 172, 276, 336 |
| BlpI | GCTNAGC | 1 cut(s) | 167 |
| BlsI | GCNGC | 3 cut(s) | 173, 277, 337 |
| BmrI | ACTGGG | 1 cut(s) | 407 |
| BmsI | GCATC | 1 cut(s) | 273 |
| BmuI | ACTGGG | 1 cut(s) | 407 |
| BpiI | GAAGAC | 1 cut(s) | 216 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 167 |
| BsaJI | CCNNGG | 1 cut(s) | 434 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 393, 423 |
| Bse1I | ACTGG | 1 cut(s) | 413 |
| BseDI | CCNNGG | 1 cut(s) | 434 |
| BseGI | GGATG | 3 cut(s) | 70, 220, 429 |
| BseNI | ACTGG | 1 cut(s) | 413 |
| BseXI | GCAGC | 3 cut(s) | 183, 287, 322 |
| Bsh1236I | CGCG | 1 cut(s) | 12 |
| BshFI | GGCC | 1 cut(s) | 420 |
| BsnI | GGCC | 1 cut(s) | 420 |
| Bsp143I | GATC | 1 cut(s) | 207 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 167 |
| BspACI | CCGC | 1 cut(s) | 278 |
| BspANI | GGCC | 1 cut(s) | 420 |
| BspFNI | CGCG | 1 cut(s) | 12 |
| BsrI | ACTGG | 1 cut(s) | 413 |
| BssECI | CCNNGG | 1 cut(s) | 434 |
| BssMI | GATC | 1 cut(s) | 207 |
| Bst4CI | ACNGT | 3 cut(s) | 7, 438, 448 |
| Bst6I | CTCTTC | 1 cut(s) | 30 |
| BstDEI | CTNAG | 1 cut(s) | 167 |
| BstDSI | CCRYGG | 1 cut(s) | 434 |
| BstF5I | GGATG | 3 cut(s) | 70, 220, 429 |
| BstFNI | CGCG | 1 cut(s) | 12 |
| BstHHI | GCGC | 1 cut(s) | 12 |
| BstKTI | GATC | 1 cut(s) | 210 |
| BstMBI | GATC | 1 cut(s) | 207 |
| BstUI | CGCG | 1 cut(s) | 12 |
| BstV1I | GCAGC | 3 cut(s) | 183, 287, 322 |
| BstV2I | GAAGAC | 1 cut(s) | 216 |
| BsuRI | GGCC | 1 cut(s) | 420 |
| BtgI | CCRYGG | 1 cut(s) | 434 |
| BtsCI | GGATG | 3 cut(s) | 70, 220, 429 |
| BtsIMutI | CAGTG | 1 cut(s) | 444 |
| CfoI | GCGC | 1 cut(s) | 12 |
| Csp6I | GTAC | 2 cut(s) | 305, 388 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 142, 177 |
| CviAII | CATG | 5 cut(s) | 84, 230, 302, 331, 340 |
| CviJI | RGCY | 4 cut(s) | 166, 174, 335, 420 |
| CviKI_1 | RGCY | 4 cut(s) | 166, 174, 335, 420 |
| CviQI | GTAC | 2 cut(s) | 305, 388 |
| DdeI | CTNAG | 1 cut(s) | 167 |
| DpnI | GATC | 1 cut(s) | 209 |
| DpnII | GATC | 1 cut(s) | 207 |
| DraIII | CACNNNGTG | 1 cut(s) | 233 |
| EaeI | YGGCCR | 1 cut(s) | 418 |
| Eam1104I | CTCTTC | 1 cut(s) | 30 |
| EarI | CTCTTC | 1 cut(s) | 30 |
| Eco32I | GATATC | 1 cut(s) | 81 |
| EcoRV | GATATC | 1 cut(s) | 81 |
| FaeI | CATG | 5 cut(s) | 87, 233, 305, 334, 343 |
| FatI | CATG | 5 cut(s) | 83, 229, 301, 330, 339 |
| Fnu4HI | GCNGC | 3 cut(s) | 172, 276, 336 |
| FokI | GGATG | 3 cut(s) | 77, 227, 416 |
| Fsp4HI | GCNGC | 3 cut(s) | 172, 276, 336 |
| FspBI | CTAG | 1 cut(s) | 344 |
| GlaI | GCGC | 1 cut(s) | 11 |
| GluI | GCNGC | 3 cut(s) | 172, 276, 336 |
| HaeIII | GGCC | 1 cut(s) | 420 |
| HhaI | GCGC | 1 cut(s) | 12 |
| Hin1II | CATG | 5 cut(s) | 87, 233, 305, 334, 343 |
| Hin6I | GCGC | 1 cut(s) | 10 |
| HinP1I | GCGC | 1 cut(s) | 10 |
| HinfI | GANTC | 5 cut(s) | 17, 38, 221, 269, 298 |
| HphI | GGTGA | 1 cut(s) | 245 |
| Hpy166II | GTNNAC | 3 cut(s) | 243, 388, 398 |
| Hpy188I | TCNGA | 5 cut(s) | 22, 43, 58, 297, 427 |
| Hpy188III | TCNNGA | 1 cut(s) | 370 |
| Hpy8I | GTNNAC | 3 cut(s) | 243, 388, 398 |
| HpyCH4III | ACNGT | 3 cut(s) | 7, 438, 448 |
| HpyCH4V | TGCA | 2 cut(s) | 93, 197 |
| HpyF3I | CTNAG | 1 cut(s) | 167 |
| Hsp92II | CATG | 5 cut(s) | 87, 233, 305, 334, 343 |
| HspAI | GCGC | 1 cut(s) | 10 |
| Kzo9I | GATC | 1 cut(s) | 207 |
| LpnPI | CCDG | 3 cut(s) | 334, 418, 426 |
| Lsp1109I | GCAGC | 3 cut(s) | 183, 287, 322 |
| LweI | GCATC | 1 cut(s) | 273 |
| MaeI | CTAG | 1 cut(s) | 344 |
| MalI | GATC | 1 cut(s) | 209 |
| MboI | GATC | 1 cut(s) | 207 |
| MboII | GAAGA | 3 cut(s) | 47, 216, 260 |
| MlsI | TGGCCA | 1 cut(s) | 420 |
| MluCI | AATT | 3 cut(s) | 94, 260, 264 |
| MluNI | TGGCCA | 1 cut(s) | 420 |
| MlyI | GAGTC | 2 cut(s) | 47, 230 |
| MnlI | CCTC | 3 cut(s) | 7, 95, 419 |
| Mox20I | TGGCCA | 1 cut(s) | 420 |
| MscI | TGGCCA | 1 cut(s) | 420 |
| MseI | TTAA | 1 cut(s) | 263 |
| Msp20I | TGGCCA | 1 cut(s) | 420 |
| MspA1I | CMGCKG | 1 cut(s) | 278 |
| MvnI | CGCG | 1 cut(s) | 12 |
| NdeII | GATC | 1 cut(s) | 207 |
| NlaIII | CATG | 5 cut(s) | 87, 233, 305, 334, 343 |
| PfeI | GAWTC | 3 cut(s) | 17, 269, 298 |
| PkrI | GCNGC | 3 cut(s) | 173, 277, 337 |
| PleI | GAGTC | 2 cut(s) | 46, 229 |
| PpsI | GAGTC | 2 cut(s) | 46, 229 |
| PshBI | ATTAAT | 1 cut(s) | 263 |
| RsaI | GTAC | 2 cut(s) | 306, 389 |
| RsaNI | GTAC | 2 cut(s) | 305, 388 |
| SaqAI | TTAA | 1 cut(s) | 263 |
| SatI | GCNGC | 3 cut(s) | 172, 276, 336 |
| Sau3AI | GATC | 1 cut(s) | 207 |
| SchI | GAGTC | 2 cut(s) | 47, 230 |
| SetI | ASST | 1 cut(s) | 159 |
| SfaNI | GCATC | 1 cut(s) | 273 |
| Sse9I | AATT | 3 cut(s) | 94, 260, 264 |
| SsiI | CCGC | 1 cut(s) | 278 |
| SspI | AATATT | 1 cut(s) | 457 |
| SspMI | CTAG | 1 cut(s) | 344 |
| TaaI | ACNGT | 3 cut(s) | 7, 438, 448 |
| TasI | AATT | 3 cut(s) | 94, 260, 264 |
| TatI | WGTACW | 1 cut(s) | 387 |
| TfiI | GAWTC | 3 cut(s) | 17, 269, 298 |
| Tru1I | TTAA | 1 cut(s) | 263 |
| Tru9I | TTAA | 1 cut(s) | 263 |
| TscAI | CASTG | 1 cut(s) | 451 |
| TseI | GCWGC | 3 cut(s) | 171, 275, 335 |
| TspDTI | ATGAA | 2 cut(s) | 273, 290 |
| TspGWI | ACGGA | 2 cut(s) | 171, 260 |
| TspRI | CASTG | 1 cut(s) | 451 |
| VspI | ATTAAT | 1 cut(s) | 263 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 328 |
| XspI | CTAG | 1 cut(s) | 344 |