MD03G1057800.v1.1

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005419
Physical Location & Seq
Reverse (-)
4636487 .. 4636945
459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1057800.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGAGGGAGAAGATTCCGAAGAAGCCAAAAACAAAACGACAGTGACGGCTTTATACCAAGCCTTAACCTCCAAGGACGTTGATGTCGTCCACCTTCTCCTCGCACCCTACCTCGAGTGGTGGTTCCATGGCCCCCCAACTCACCAGCATCTGAACCGCCTACTCACTGGTGCACCACCGTATGACTCATCGTTCAAATTCGTCCCTCTCTCGATCATTGCATTTGGATCAATGGTGCTGGCTGAAGGATACGACGAGGTTCGTTCCGTTTCGTGGGTGCATGCGTGGATCTTCACTGATGGGATTATTACCCAGGTCAGGGAATACTACAACACGTCCGTCACGGTCACCCGGTTATCCTCACCGCATATCAGATCACAACCGGGGAATTGCCAGTGTCTCTGGCAGAGTAAGCTCTCGGATAACAAGTCTGTGCCTGGCCTCGTTTTGGCGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WI12

Protein Analysis

153

Amino Acids

17.06

Weight (kDa)

5.8

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 53 - 152 1.2e-37 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AciI CCGC 2 cut(s) 157, 365
AclWI GGATC 2 cut(s) 235, 296
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 1 cut(s) 264
AfiI CCNNNNNNNGG 4 cut(s) 273, 318, 319, 448
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 1 cut(s) 196
AjiI CACGTC 1 cut(s) 336
AjnI CCWGG 2 cut(s) 312, 436
AluBI AGCT 1 cut(s) 415
AluI AGCT 1 cut(s) 415
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 1 cut(s) 404
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 2 cut(s) 235, 296
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 2 cut(s) 130, 439
ApaLI GTGCAC 1 cut(s) 171
ApoI RAATTY 1 cut(s) 197
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 131
AsuC2I CCSGG 2 cut(s) 352, 384
AsuHPI GGTGA 3 cut(s) 134, 340, 354
AvaI CYCGRG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 175
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 63
BciT130I CCWGG 2 cut(s) 314, 438
BciVI GTATCC 1 cut(s) 242
BcnI CCSGG 2 cut(s) 352, 384
BcoDI GTCTC 1 cut(s) 404
BfoI RGCGCY 1 cut(s) 455
BfuI GTATCC 1 cut(s) 242
Bme1390I CCNGG 4 cut(s) 314, 352, 384, 438
BmeT110I CYCGRG 1 cut(s) 113
BmgBI CACGTC 1 cut(s) 336
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 125, 133
BmrFI CCNGG 4 cut(s) 314, 352, 384, 438
BmsI GCATC 1 cut(s) 157
BpuMI CCSGG 2 cut(s) 352, 384
BsaJI CCNNGG 4 cut(s) 72, 127, 312, 383
Bsc4I CCNNNNNNNGG 4 cut(s) 273, 318, 319, 448
Bse1I ACTGG 2 cut(s) 172, 394
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 2 cut(s) 314, 438
BseDI CCNNGG 4 cut(s) 72, 127, 312, 383
BseLI CCNNNNNNNGG 4 cut(s) 273, 318, 319, 448
BseMI GCAATG 1 cut(s) 216
BseNI ACTGG 2 cut(s) 172, 394
BseRI GAGGAG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 175
BshFI GGCC 2 cut(s) 132, 441
BsiHKAI GWGCWC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 2 cut(s) 352, 383
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 4 cut(s) 273, 318, 319, 448
BsmAI GTCTC 1 cut(s) 404
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 2 cut(s) 132, 441
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 4 cut(s) 213, 227, 288, 374
Bsp19I CCATGG 1 cut(s) 127
BspACI CCGC 2 cut(s) 157, 365
BspANI GGCC 2 cut(s) 132, 441
BspLI GGNNCC 2 cut(s) 125, 133
BspPI GGATC 2 cut(s) 235, 296
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 2 cut(s) 172, 394
BssECI CCNNGG 4 cut(s) 72, 127, 312, 383
BssMI GATC 4 cut(s) 213, 227, 288, 374
BssT1I CCWWGG 2 cut(s) 72, 127
Bst2UI CCWGG 2 cut(s) 314, 438
Bst4CI ACNGT 3 cut(s) 43, 180, 346
BstC8I GCNNGC 2 cut(s) 240, 282
BstDSI CCRYGG 1 cut(s) 127
BstEII GGTNACC 1 cut(s) 346
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 4 cut(s) 216, 230, 291, 377
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 4 cut(s) 213, 227, 288, 374
BstMWI GCNNNNNNNGC 1 cut(s) 412
BstNI CCWGG 2 cut(s) 314, 438
BstNSI RCATGY 1 cut(s) 284
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 4 cut(s) 312, 350, 382, 436
BstSLI GKGCMC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 2 cut(s) 132, 441
BtgI CCRYGG 1 cut(s) 127
BtrI CACGTC 1 cut(s) 336
BtsIMutI CAGTG 4 cut(s) 48, 165, 294, 401
Cac8I GCNNGC 2 cut(s) 240, 282
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 131
CviAII CATG 2 cut(s) 128, 281
CviJI RGCY 7 cut(s) 26, 50, 62, 132, 242, 415, 441
CviKI_1 RGCY 7 cut(s) 26, 50, 62, 132, 242, 415, 441
DpnI GATC 4 cut(s) 215, 229, 290, 376
DpnII GATC 4 cut(s) 213, 227, 288, 374
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
Eco130I CCWWGG 2 cut(s) 72, 127
Eco57I CTGAAG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 113
Eco91I GGTNACC 1 cut(s) 346
EcoO65I GGTNACC 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 312, 436
EcoT14I CCWWGG 2 cut(s) 72, 127
ErhI CCWWGG 2 cut(s) 72, 127
FaeI CATG 2 cut(s) 131, 284
FaiI YATR 6 cut(s) 55, 129, 183, 282, 369, 457
FaqI GGGAC 1 cut(s) 188
FatI CATG 2 cut(s) 127, 280
GlaI GCGC 1 cut(s) 453
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 2 cut(s) 132, 441
HapII CCGG 2 cut(s) 352, 383
HhaI GCGC 1 cut(s) 454
Hin1II CATG 2 cut(s) 131, 284
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HinfI GANTC 2 cut(s) 14, 185
HpaII CCGG 2 cut(s) 352, 383
HphI GGTGA 3 cut(s) 134, 340, 354
Hpy166II GTNNAC 2 cut(s) 91, 173
Hpy188I TCNGA 4 cut(s) 19, 153, 374, 421
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 2 cut(s) 91, 173
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 2 cut(s) 104, 239
HpyCH4III ACNGT 3 cut(s) 43, 180, 346
HpyCH4IV ACGT 2 cut(s) 78, 335
HpyCH4V TGCA 3 cut(s) 173, 221, 280
HpyF10VI GCNNNNNNNGC 1 cut(s) 412
HpySE526I ACGT 2 cut(s) 78, 335
Hsp92II CATG 2 cut(s) 131, 284
HspAI GCGC 1 cut(s) 452
Kzo9I GATC 4 cut(s) 213, 227, 288, 374
LweI GCATC 1 cut(s) 157
MaeII ACGT 2 cut(s) 78, 335
MaeIII GTNAC 3 cut(s) 43, 340, 346
MalI GATC 4 cut(s) 215, 229, 290, 376
MboI GATC 4 cut(s) 213, 227, 288, 374
MboII GAAGA 3 cut(s) 23, 32, 283
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 2 cut(s) 197, 388
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 7 cut(s) 79, 110, 122, 216, 250, 370, 452
MseI TTAA 1 cut(s) 65
MspI CCGG 2 cut(s) 352, 383
MspR9I CCNGG 4 cut(s) 314, 352, 384, 438
MvaI CCWGG 2 cut(s) 314, 438
MwoI GCNNNNNNNGC 1 cut(s) 412
NciI CCSGG 2 cut(s) 352, 384
NcoI CCATGG 1 cut(s) 127
NdeII GATC 4 cut(s) 213, 227, 288, 374
NlaIII CATG 2 cut(s) 131, 284
NlaIV GGNNCC 2 cut(s) 125, 133
NmuCI GTSAC 3 cut(s) 43, 340, 346
NspI RCATGY 1 cut(s) 284
PaeI GCATGC 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 14
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp6I CCWGG 2 cut(s) 312, 436
PspEI GGTNACC 1 cut(s) 346
PspGI CCWGG 2 cut(s) 312, 436
PspN4I GGNNCC 2 cut(s) 125, 133
PspPI GGNCC 1 cut(s) 131
PspXI VCTCGAGB 1 cut(s) 113
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 288
SaqAI TTAA 1 cut(s) 65
Sau3AI GATC 4 cut(s) 213, 227, 288, 374
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 4 cut(s) 314, 352, 384, 438
SduI GDGCHC 1 cut(s) 175
SetI ASST 8 cut(s) 71, 81, 96, 114, 261, 318, 338, 417
SfaNI GCATC 1 cut(s) 157
Sfr274I CTCGAG 1 cut(s) 113
SlaI CTCGAG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SphI GCATGC 1 cut(s) 284
Sse9I AATT 2 cut(s) 197, 388
SsiI CCGC 2 cut(s) 157, 365
StyD4I CCNGG 4 cut(s) 312, 350, 382, 436
StyI CCWWGG 2 cut(s) 72, 127
TaaI ACNGT 3 cut(s) 43, 180, 346
TaiI ACGT 2 cut(s) 81, 338
TaqI TCGA 2 cut(s) 114, 212
TasI AATT 2 cut(s) 197, 388
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TscAI CASTG 4 cut(s) 48, 172, 301, 401
TseFI GTSAC 3 cut(s) 43, 340, 346
Tsp45I GTSAC 3 cut(s) 43, 340, 346
TspGWI ACGGA 2 cut(s) 256, 328
TspRI CASTG 4 cut(s) 48, 172, 301, 401
VneI GTGCAC 1 cut(s) 171
XapI RAATTY 1 cut(s) 197
XceI RCATGY 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.