Rw1G032890

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005419
Physical Location & Seq
Reverse (-)
61093668 .. 61094844
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032890.1

Sequence Viewer

Length: 495 bp
ATGAATGACTCGTGTATAACTTTTATCCAGCTGGTTCTGAAACTCTATGACGCCTTGAGCACCCGTGACGTGGCCACCGTGCACGCCGTCCTCGCCGCCGACCTCGAGTGGTGGTTCCATGGTCCGCCGACCCACCAGTTCTTGAAGCGCCTGCTGACCGGCTGCGACAACAAGAACGACGCGTCGTTTCAGTTCCATCCCCAGTCTATTTGCTCCGTCGGCCCCGTCGTCCTAGTGGAGGGCTGTGACCCAGAGCAGGAGATCTCCTGGGTACACGCCTGGACCGTCACGGATGGGATAATCACCCAGGTCAGGGAGTACTTCAACACTTCCCTCACCGTCACCCGCCTCGGGAACTCCACCACCTCCGCCAAACCCTCCCCGTCCAAGTTCTCCAAACCGACGTCCGAGATTACATCGTACCACTGCCCCTCCGTCTGGGAGAGCTCCAACCGGGTCGGGAAGTCGGTTCCGGGTCTGGTCCTCGCAATCTAA

Protein Analysis

164

Amino Acids

18.12

Weight (kDa)

6.03

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 50 - 164 4.1e-41 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
AccII CGCG 1 cut(s) 182
AciI CCGC 4 cut(s) 96, 125, 346, 369
AcoI YGGCCR 1 cut(s) 72
AcyI GRCGYC 2 cut(s) 51, 404
AfaI GTAC 3 cut(s) 273, 320, 422
AfiI CCNNNNNNNGG 7 cut(s) 70, 238, 256, 312, 313, 351, 438
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 2 cut(s) 145, 325
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 3 cut(s) 266, 278, 306
AluBI AGCT 2 cut(s) 31, 447
AluI AGCT 2 cut(s) 31, 447
Alw21I GWGCWC 3 cut(s) 62, 84, 449
Alw44I GTGCAC 1 cut(s) 80
Ama87I CYCGRG 2 cut(s) 104, 350
AoxI GGCC 2 cut(s) 72, 220
ApaLI GTGCAC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 162
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 4 cut(s) 122, 221, 282, 481
AsuC2I CCSGG 2 cut(s) 455, 474
AsuHPI GGTGA 3 cut(s) 295, 328, 334
AvaI CYCGRG 2 cut(s) 104, 350
AvaII GGWCC 3 cut(s) 122, 282, 481
BaeGI GKGCMC 1 cut(s) 84
BalI TGGCCA 1 cut(s) 74
BanII GRGCYC 1 cut(s) 449
BauI CACGAG 1 cut(s) 10
Bbv12I GWGCWC 3 cut(s) 62, 84, 449
BbvI GCAGC 1 cut(s) 149
BccI CCATC 2 cut(s) 204, 287
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 3 cut(s) 268, 280, 308
BcnI CCSGG 2 cut(s) 455, 474
BfaI CTAG 1 cut(s) 233
BfoI RGCGCY 1 cut(s) 151
BglII AGATCT 1 cut(s) 261
BisI GCNGC 2 cut(s) 96, 163
BlsI GCNGC 2 cut(s) 97, 164
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 5 cut(s) 268, 280, 308, 455, 474
Bme18I GGWCC 3 cut(s) 122, 282, 481
BmeT110I CYCGRG 2 cut(s) 104, 350
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 122, 221, 282, 481
BmiI GGNNCC 3 cut(s) 116, 223, 471
BmrFI CCNGG 5 cut(s) 268, 280, 308, 455, 474
BmrI ACTGGG 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 455, 474
BsaHI GRCGYC 2 cut(s) 51, 404
BsaJI CCNNGG 4 cut(s) 118, 267, 306, 349
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 238, 256, 312, 313, 351, 438
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 136, 202
BseBI CCWGG 3 cut(s) 268, 280, 308
BseDI CCNNGG 4 cut(s) 118, 267, 306, 349
BseGI GGATG 2 cut(s) 196, 298
BseLI CCNNNNNNNGG 7 cut(s) 70, 238, 256, 312, 313, 351, 438
BseNI ACTGG 2 cut(s) 136, 202
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 74, 222
BsiHKAI GWGCWC 3 cut(s) 62, 84, 449
BsiHKCI CYCGRG 2 cut(s) 104, 350
BsiSI CCGG 3 cut(s) 159, 454, 473
BslI CCNNNNNNNGG 7 cut(s) 70, 238, 256, 312, 313, 351, 438
BsnI GGCC 2 cut(s) 74, 222
BsoBI CYCGRG 2 cut(s) 104, 350
Bsp1286I GDGCHC 3 cut(s) 62, 84, 449
Bsp143I GATC 1 cut(s) 261
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 4 cut(s) 96, 125, 346, 369
BspANI GGCC 2 cut(s) 74, 222
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 3 cut(s) 116, 223, 471
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 2 cut(s) 136, 202
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 4 cut(s) 118, 267, 306, 349
BssMI GATC 1 cut(s) 261
BssNI GRCGYC 2 cut(s) 51, 404
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 3 cut(s) 268, 280, 308
Bst4CI ACNGT 3 cut(s) 79, 286, 340
BstACI GRCGYC 2 cut(s) 51, 404
BstC8I GCNNGC 2 cut(s) 84, 152
BstDSI CCRYGG 1 cut(s) 118
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 2 cut(s) 196, 298
BstFNI CGCG 1 cut(s) 182
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 1 cut(s) 264
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 2 cut(s) 92, 219
BstNI CCWGG 3 cut(s) 268, 280, 308
BstSCI CCNGG 5 cut(s) 266, 278, 306, 453, 472
BstSLI GKGCMC 1 cut(s) 84
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 2 cut(s) 74, 222
BtgI CCRYGG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 2 cut(s) 196, 298
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 424
Cac8I GCNNGC 2 cut(s) 84, 152
CfoI GCGC 1 cut(s) 150
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 4 cut(s) 122, 221, 282, 481
CseI GACGC 3 cut(s) 59, 171, 188
Csp6I GTAC 3 cut(s) 272, 319, 421
CviAII CATG 1 cut(s) 119
CviJI RGCY 6 cut(s) 31, 74, 162, 222, 243, 447
CviKI_1 RGCY 6 cut(s) 31, 74, 162, 222, 243, 447
CviQI GTAC 3 cut(s) 272, 319, 421
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
EaeI YGGCCR 1 cut(s) 72
EciI GGCGGA 2 cut(s) 114, 358
Ecl136II GAGCTC 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 449
Eco47I GGWCC 3 cut(s) 122, 282, 481
Eco53kI GAGCTC 1 cut(s) 447
Eco88I CYCGRG 2 cut(s) 104, 350
EcoICRI GAGCTC 1 cut(s) 447
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoRII CCWGG 3 cut(s) 266, 278, 306
EcoT14I CCWWGG 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 1 cut(s) 122
FaiI YATR 3 cut(s) 17, 48, 120
FatI CATG 1 cut(s) 118
FauI CCCGC 1 cut(s) 353
Fnu4HI GCNGC 2 cut(s) 96, 163
FokI GGATG 2 cut(s) 183, 305
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 2 cut(s) 96, 163
FspBI CTAG 1 cut(s) 233
GlaI GCGC 1 cut(s) 149
GluI GCNGC 2 cut(s) 96, 163
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 2 cut(s) 74, 222
HapII CCGG 3 cut(s) 159, 454, 473
HgaI GACGC 3 cut(s) 59, 171, 188
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 2 cut(s) 51, 404
Hin1II CATG 1 cut(s) 122
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HinfI GANTC 1 cut(s) 8
HpaII CCGG 3 cut(s) 159, 454, 473
HphI GGTGA 3 cut(s) 295, 328, 334
Hpy166II GTNNAC 2 cut(s) 82, 274
Hpy188I TCNGA 2 cut(s) 39, 409
Hpy188III TCNNGA 3 cut(s) 142, 352, 460
Hpy8I GTNNAC 2 cut(s) 82, 274
Hpy99I CGWCG 5 cut(s) 182, 187, 221, 230, 406
HpyCH4III ACNGT 3 cut(s) 79, 286, 340
HpyCH4IV ACGT 2 cut(s) 69, 404
HpyCH4V TGCA 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 219
HpySE526I ACGT 2 cut(s) 69, 404
Hsp92I GRCGYC 2 cut(s) 51, 404
Hsp92II CATG 1 cut(s) 122
HspAI GCGC 1 cut(s) 148
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 2 cut(s) 218, 452
Lsp1109I GCAGC 1 cut(s) 149
MaeI CTAG 1 cut(s) 233
MaeII ACGT 2 cut(s) 69, 404
MaeIII GTNAC 4 cut(s) 65, 245, 286, 340
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 3 cut(s) 62, 84, 449
MlsI TGGCCA 1 cut(s) 74
MluI ACGCGT 1 cut(s) 180
MluNI TGGCCA 1 cut(s) 74
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 9 cut(s) 101, 113, 232, 344, 359, 376, 388, 442, 494
Mox20I TGGCCA 1 cut(s) 74
MscI TGGCCA 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 3 cut(s) 159, 454, 473
MspR9I CCNGG 5 cut(s) 268, 280, 308, 455, 474
MvaI CCWGG 3 cut(s) 268, 280, 308
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 2 cut(s) 92, 219
NciI CCSGG 2 cut(s) 455, 474
NcoI CCATGG 1 cut(s) 118
NdeII GATC 1 cut(s) 261
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 3 cut(s) 116, 223, 471
NmuCI GTSAC 4 cut(s) 65, 245, 286, 340
PaeR7I CTCGAG 1 cut(s) 104
PcsI WCGNNNNNNNCGW 3 cut(s) 75, 225, 282
PkrI GCNGC 2 cut(s) 97, 164
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 3 cut(s) 266, 278, 306
PspGI CCWGG 3 cut(s) 266, 278, 306
PspN4I GGNNCC 3 cut(s) 116, 223, 471
PspPI GGNCC 4 cut(s) 122, 221, 282, 481
PspXI VCTCGAGB 1 cut(s) 104
PsuI RGATCY 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 3 cut(s) 273, 320, 422
RsaNI GTAC 3 cut(s) 272, 319, 421
SacI GAGCTC 1 cut(s) 449
SatI GCNGC 2 cut(s) 96, 163
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 4 cut(s) 122, 221, 282, 481
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 5 cut(s) 268, 280, 308, 455, 474
SduI GDGCHC 3 cut(s) 62, 84, 449
SetI ASST 7 cut(s) 33, 72, 105, 312, 368, 407, 449
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 3 cut(s) 122, 282, 481
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 55, 104
SmoI CTYRAG 2 cut(s) 55, 104
SsiI CCGC 4 cut(s) 96, 125, 346, 369
SspMI CTAG 1 cut(s) 233
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 5 cut(s) 266, 278, 306, 453, 472
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 79, 286, 340
TaiI ACGT 2 cut(s) 72, 407
TaqI TCGA 1 cut(s) 105
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 98
TscAI CASTG 1 cut(s) 431
TseFI GTSAC 4 cut(s) 65, 245, 286, 340
TseI GCWGC 1 cut(s) 162
Tsp45I GTSAC 4 cut(s) 65, 245, 286, 340
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 205, 305, 424
TspRI CASTG 1 cut(s) 431
VneI GTGCAC 1 cut(s) 80
VpaK11BI GGWCC 3 cut(s) 122, 282, 481
XagI CCTNNNNNAGG 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 1 cut(s) 233
ZraI GACGTC 1 cut(s) 405
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.