Rh1AG373900

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005419
Physical Location & Seq
Reverse (-)
60702075 .. 60704741
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG373900.1

Sequence Viewer

Length: 489 bp
ATGGTTAAAAATGACAATCAGCAGCTGGTTCTGAAACTCTATGACGCCTTGAGCACCCGTGACGTGGCCACCGTGCACGCCGTCCTCGCCGCCGACCTCGAGTGGTGGTTCCATGGTCCGCCGACCCACCAGTTCTTGAAGCGCCTGCTGACCGGCTGCGACAACAAGAACGACGCGTCGTTTCAGTTCCATCCCCAGTCTATTTGCTCCGTCGGCCCCGTCGTCCTAGTGGAGGGCTGTGACCCAGAGCAGGAGATCTCCTGGGTACACGCCTGGACCGTCACGGATGGGATAATCACCCAGGTCAGGGAGTACTTCAACACTTCCCTCACCGTCACCCGCCTCGGGAACTCCACCAGCTCCGCCAAACCCGCCCCGTCCAAGTTCTCCAAGTCGACGTCCGAGATTACATCGTACCACTGCCCCTCCGTCTGGGAGAGCTCCAACCGGGTCGGGAAGTCGGTTCCGGGTCTGGTCCTCGCAATCTAA

Protein Analysis

162

Amino Acids

17.89

Weight (kDa)

6.28

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 48 - 162 2.7e-41 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 395
AccII CGCG 1 cut(s) 176
AciI CCGC 5 cut(s) 90, 119, 340, 363, 372
AcoI YGGCCR 1 cut(s) 66
AcyI GRCGYC 2 cut(s) 45, 398
AfaI GTAC 3 cut(s) 267, 314, 416
AfiI CCNNNNNNNGG 7 cut(s) 64, 232, 250, 306, 307, 345, 432
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 2 cut(s) 139, 319
AjiI CACGTC 1 cut(s) 64
AjnI CCWGG 3 cut(s) 260, 272, 300
AluBI AGCT 3 cut(s) 25, 360, 441
AluI AGCT 3 cut(s) 25, 360, 441
Alw21I GWGCWC 3 cut(s) 56, 78, 443
Alw44I GTGCAC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 2 cut(s) 98, 344
AoxI GGCC 2 cut(s) 66, 214
ApaLI GTGCAC 1 cut(s) 74
ApeKI GCWGC 2 cut(s) 22, 156
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 4 cut(s) 116, 215, 276, 475
AsuC2I CCSGG 2 cut(s) 449, 468
AsuHPI GGTGA 3 cut(s) 289, 322, 328
AvaI CYCGRG 2 cut(s) 98, 344
AvaII GGWCC 3 cut(s) 116, 276, 475
BaeGI GKGCMC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 68
BanII GRGCYC 1 cut(s) 443
Bbv12I GWGCWC 3 cut(s) 56, 78, 443
BbvI GCAGC 2 cut(s) 34, 143
BccI CCATC 2 cut(s) 198, 281
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 3 cut(s) 262, 274, 302
BcnI CCSGG 2 cut(s) 449, 468
BfaI CTAG 1 cut(s) 227
BfoI RGCGCY 1 cut(s) 145
BglII AGATCT 1 cut(s) 255
BisI GCNGC 3 cut(s) 23, 90, 157
BlsI GCNGC 3 cut(s) 24, 91, 158
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 5 cut(s) 262, 274, 302, 449, 468
Bme18I GGWCC 3 cut(s) 116, 276, 475
BmeT110I CYCGRG 2 cut(s) 98, 344
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 4 cut(s) 116, 215, 276, 475
BmiI GGNNCC 3 cut(s) 110, 217, 465
BmrFI CCNGG 5 cut(s) 262, 274, 302, 449, 468
BmrI ACTGGG 1 cut(s) 190
BmuI ACTGGG 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 70
BpuMI CCSGG 2 cut(s) 449, 468
BsaHI GRCGYC 2 cut(s) 45, 398
BsaJI CCNNGG 4 cut(s) 112, 261, 300, 343
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 232, 250, 306, 307, 345, 432
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 130, 196
BseBI CCWGG 3 cut(s) 262, 274, 302
BseDI CCNNGG 4 cut(s) 112, 261, 300, 343
BseGI GGATG 2 cut(s) 190, 292
BseLI CCNNNNNNNGG 7 cut(s) 64, 232, 250, 306, 307, 345, 432
BseNI ACTGG 2 cut(s) 130, 196
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 2 cut(s) 34, 143
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 68, 216
BsiHKAI GWGCWC 3 cut(s) 56, 78, 443
BsiHKCI CYCGRG 2 cut(s) 98, 344
BsiSI CCGG 3 cut(s) 153, 448, 467
BslI CCNNNNNNNGG 7 cut(s) 64, 232, 250, 306, 307, 345, 432
BsnI GGCC 2 cut(s) 68, 216
BsoBI CYCGRG 2 cut(s) 98, 344
Bsp1286I GDGCHC 3 cut(s) 56, 78, 443
Bsp143I GATC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 5 cut(s) 90, 119, 340, 363, 372
BspANI GGCC 2 cut(s) 68, 216
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 3 cut(s) 110, 217, 465
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 130, 196
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 4 cut(s) 112, 261, 300, 343
BssMI GATC 1 cut(s) 255
BssNI GRCGYC 2 cut(s) 45, 398
BssT1I CCWWGG 1 cut(s) 112
Bst2UI CCWGG 3 cut(s) 262, 274, 302
Bst4CI ACNGT 3 cut(s) 73, 280, 334
BstACI GRCGYC 2 cut(s) 45, 398
BstC8I GCNNGC 2 cut(s) 78, 146
BstDSI CCRYGG 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 2 cut(s) 190, 292
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 1 cut(s) 258
BstMBI GATC 1 cut(s) 255
BstMWI GCNNNNNNNGC 3 cut(s) 86, 213, 371
BstNI CCWGG 3 cut(s) 262, 274, 302
BstSCI CCNGG 5 cut(s) 260, 272, 300, 447, 466
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 34, 143
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BsuRI GGCC 2 cut(s) 68, 216
BtgI CCRYGG 1 cut(s) 112
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 2 cut(s) 190, 292
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 1 cut(s) 418
Cac8I GCNNGC 2 cut(s) 78, 146
CaiI CAGNNNCTG 1 cut(s) 25
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 4 cut(s) 116, 215, 276, 475
CseI GACGC 3 cut(s) 53, 165, 182
Csp6I GTAC 3 cut(s) 266, 313, 415
CviAII CATG 1 cut(s) 113
CviJI RGCY 7 cut(s) 25, 68, 156, 216, 237, 360, 441
CviKI_1 RGCY 7 cut(s) 25, 68, 156, 216, 237, 360, 441
CviQI GTAC 3 cut(s) 266, 313, 415
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
EaeI YGGCCR 1 cut(s) 66
EciI GGCGGA 2 cut(s) 108, 352
Ecl136II GAGCTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 443
Eco47I GGWCC 3 cut(s) 116, 276, 475
Eco53kI GAGCTC 1 cut(s) 441
Eco88I CYCGRG 2 cut(s) 98, 344
EcoICRI GAGCTC 1 cut(s) 441
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoRII CCWGG 3 cut(s) 260, 272, 300
EcoT14I CCWWGG 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 1 cut(s) 116
FaiI YATR 2 cut(s) 42, 114
FatI CATG 1 cut(s) 112
FauI CCCGC 2 cut(s) 347, 379
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 3 cut(s) 23, 90, 157
FokI GGATG 2 cut(s) 177, 299
FriOI GRGCYC 1 cut(s) 443
Fsp4HI GCNGC 3 cut(s) 23, 90, 157
FspBI CTAG 1 cut(s) 227
GlaI GCGC 1 cut(s) 143
GluI GCNGC 3 cut(s) 23, 90, 157
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 2 cut(s) 68, 216
HapII CCGG 3 cut(s) 153, 448, 467
HgaI GACGC 3 cut(s) 53, 165, 182
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 2 cut(s) 45, 398
Hin1II CATG 1 cut(s) 116
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HpaII CCGG 3 cut(s) 153, 448, 467
HphI GGTGA 3 cut(s) 289, 322, 328
Hpy166II GTNNAC 3 cut(s) 76, 268, 396
Hpy188I TCNGA 2 cut(s) 33, 403
Hpy188III TCNNGA 3 cut(s) 136, 346, 454
Hpy8I GTNNAC 3 cut(s) 76, 268, 396
Hpy99I CGWCG 5 cut(s) 176, 181, 215, 224, 400
HpyCH4III ACNGT 3 cut(s) 73, 280, 334
HpyCH4IV ACGT 2 cut(s) 63, 398
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 213, 371
HpySE526I ACGT 2 cut(s) 63, 398
Hsp92I GRCGYC 2 cut(s) 45, 398
Hsp92II CATG 1 cut(s) 116
HspAI GCGC 1 cut(s) 142
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 3 cut(s) 212, 365, 446
Lsp1109I GCAGC 2 cut(s) 34, 143
MaeI CTAG 1 cut(s) 227
MaeII ACGT 2 cut(s) 63, 398
MaeIII GTNAC 4 cut(s) 59, 239, 280, 334
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 3 cut(s) 56, 78, 443
MlsI TGGCCA 1 cut(s) 68
MluI ACGCGT 1 cut(s) 174
MluNI TGGCCA 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 7 cut(s) 95, 107, 226, 338, 353, 436, 488
Mox20I TGGCCA 1 cut(s) 68
MscI TGGCCA 1 cut(s) 68
MseI TTAA 1 cut(s) 6
Msp20I TGGCCA 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 25
MspI CCGG 3 cut(s) 153, 448, 467
MspR9I CCNGG 5 cut(s) 262, 274, 302, 449, 468
MvaI CCWGG 3 cut(s) 262, 274, 302
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 86, 213, 371
NciI CCSGG 2 cut(s) 449, 468
NcoI CCATGG 1 cut(s) 112
NdeII GATC 1 cut(s) 255
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 3 cut(s) 110, 217, 465
NmuCI GTSAC 4 cut(s) 59, 239, 280, 334
PaeR7I CTCGAG 1 cut(s) 98
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 219, 276
PkrI GCNGC 3 cut(s) 24, 91, 158
Psp124BI GAGCTC 1 cut(s) 443
Psp6I CCWGG 3 cut(s) 260, 272, 300
PspGI CCWGG 3 cut(s) 260, 272, 300
PspN4I GGNNCC 3 cut(s) 110, 217, 465
PspPI GGNCC 4 cut(s) 116, 215, 276, 475
PspXI VCTCGAGB 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 3 cut(s) 267, 314, 416
RsaNI GTAC 3 cut(s) 266, 313, 415
SacI GAGCTC 1 cut(s) 443
SalI GTCGAC 1 cut(s) 394
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 3 cut(s) 23, 90, 157
Sau3AI GATC 1 cut(s) 255
Sau96I GGNCC 4 cut(s) 116, 215, 276, 475
ScaI AGTACT 1 cut(s) 314
ScrFI CCNGG 5 cut(s) 262, 274, 302, 449, 468
SduI GDGCHC 3 cut(s) 56, 78, 443
SetI ASST 7 cut(s) 27, 66, 99, 306, 362, 401, 443
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 3 cut(s) 116, 276, 475
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 49, 98
SmoI CTYRAG 2 cut(s) 49, 98
SsiI CCGC 5 cut(s) 90, 119, 340, 363, 372
SspMI CTAG 1 cut(s) 227
SstI GAGCTC 1 cut(s) 443
StyD4I CCNGG 5 cut(s) 260, 272, 300, 447, 466
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 3 cut(s) 73, 280, 334
TaiI ACGT 2 cut(s) 66, 401
TaqI TCGA 2 cut(s) 99, 395
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 1 cut(s) 425
TseFI GTSAC 4 cut(s) 59, 239, 280, 334
TseI GCWGC 2 cut(s) 22, 156
Tsp45I GTSAC 4 cut(s) 59, 239, 280, 334
TspGWI ACGGA 3 cut(s) 199, 299, 418
TspRI CASTG 1 cut(s) 425
VneI GTGCAC 1 cut(s) 74
VpaK11BI GGWCC 3 cut(s) 116, 276, 475
XagI CCTNNNNNAGG 1 cut(s) 230
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 395
XspI CTAG 1 cut(s) 227
ZraI GACGTC 1 cut(s) 399
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.