RLG00000026965

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005419
Physical Location & Seq
Forward (+)
7294145 .. 7295227
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026965

Sequence Viewer

Length: 582 bp
ATGACCACGACCGGCCAAAAAGGAGTGGCGACTGACCCCGACGCCATTAGCGTAAAGCTAGAACTGGCGTTATCTCAAGAAACCGACGATTCTTCTTCCGATTCCAGCAATCACCGGCTGGTTCTGAAACTCTATGACGCCTTGAGCACCCGTGACGTGGCCACCGTGCACGCCGTCCTCGCCGCCGACCTCGAGTGGTGGTTCCATGGTCCGCCGACCCACCAGTTCTTGATGCGCCTGCTGACCGGCTGCGACAACAAAAACGACGCGTCGTTTCAGTTCAATCCTCAGTCTATTTGCTCCGTCGGCCCCGTCGTCCTAGTGGAGGGCTGTGACCCAGAGCAGGAGATCTCCTGGGTACACGCCTGGACCGTCACGGATGGGATAATCACCCAGGTCAGGGAGTACTTCAACACTTCCCTCACCGTCACCCGCCTCGGGAACTCCACCACCTCCGCCAAATCCACACCGTCCAAGTTCTCCAAGTCGACGTCCGAGATTACATCGTACCACTGCCCCTCCGTCTGGGAGAGCTCCAACCGGGTCGGGAAGTCGGTTCCGGGTCTGGTCCTCGCAATCTAA

Protein Analysis

194

Amino Acids

21.02

Weight (kDa)

5.25

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 78 - 193 1.1e-42 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 494
AccI GTMKAC 1 cut(s) 488
AccII CGCG 1 cut(s) 269
AciI CCGC 4 cut(s) 183, 212, 433, 456
AcoI YGGCCR 2 cut(s) 13, 159
AcyI GRCGYC 3 cut(s) 42, 138, 491
AfaI GTAC 3 cut(s) 360, 407, 509
AfiI CCNNNNNNNGG 7 cut(s) 157, 325, 343, 399, 400, 438, 525
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 2 cut(s) 283, 412
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 3 cut(s) 353, 365, 393
AluBI AGCT 2 cut(s) 58, 534
AluI AGCT 2 cut(s) 58, 534
Alw21I GWGCWC 3 cut(s) 149, 171, 536
Alw44I GTGCAC 1 cut(s) 167
Ama87I CYCGRG 2 cut(s) 191, 437
AoxI GGCC 3 cut(s) 13, 159, 307
ApaLI GTGCAC 1 cut(s) 167
ApeKI GCWGC 1 cut(s) 249
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 4 cut(s) 209, 308, 369, 568
AsuC2I CCSGG 2 cut(s) 542, 561
AsuHPI GGTGA 4 cut(s) 104, 382, 415, 421
AvaI CYCGRG 2 cut(s) 191, 437
AvaII GGWCC 3 cut(s) 209, 369, 568
BaeGI GKGCMC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 161
BanII GRGCYC 1 cut(s) 536
Bbv12I GWGCWC 3 cut(s) 149, 171, 536
BbvI GCAGC 1 cut(s) 236
BccI CCATC 1 cut(s) 374
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 3 cut(s) 355, 367, 395
BcnI CCSGG 2 cut(s) 542, 561
BfaI CTAG 2 cut(s) 59, 320
BglII AGATCT 1 cut(s) 348
BisI GCNGC 2 cut(s) 183, 250
BlsI GCNGC 2 cut(s) 184, 251
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 5 cut(s) 355, 367, 395, 542, 561
Bme18I GGWCC 3 cut(s) 209, 369, 568
BmeT110I CYCGRG 2 cut(s) 191, 437
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 4 cut(s) 209, 308, 369, 568
BmiI GGNNCC 3 cut(s) 203, 310, 558
BmrFI CCNGG 5 cut(s) 355, 367, 395, 542, 561
BmsI GCATC 1 cut(s) 222
BpuEI CTTGAG 2 cut(s) 60, 163
BpuMI CCSGG 2 cut(s) 542, 561
BsaHI GRCGYC 3 cut(s) 42, 138, 491
BsaJI CCNNGG 4 cut(s) 205, 354, 393, 436
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bsc4I CCNNNNNNNGG 7 cut(s) 157, 325, 343, 399, 400, 438, 525
Bse118I RCCGGY 3 cut(s) 11, 114, 245
Bse1I ACTGG 2 cut(s) 69, 223
BseBI CCWGG 3 cut(s) 355, 367, 395
BseDI CCNNGG 4 cut(s) 205, 354, 393, 436
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 7 cut(s) 157, 325, 343, 399, 400, 438, 525
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 2 cut(s) 69, 223
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 269
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 15, 161, 309
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 3 cut(s) 149, 171, 536
BsiHKCI CYCGRG 2 cut(s) 191, 437
BsiSI CCGG 5 cut(s) 12, 115, 246, 541, 560
BslI CCNNNNNNNGG 7 cut(s) 157, 325, 343, 399, 400, 438, 525
BsnI GGCC 3 cut(s) 15, 161, 309
BsoBI CYCGRG 2 cut(s) 191, 437
Bsp1286I GDGCHC 3 cut(s) 149, 171, 536
Bsp143I GATC 1 cut(s) 348
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 4 cut(s) 183, 212, 433, 456
BspANI GGCC 3 cut(s) 15, 161, 309
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 203, 310, 558
BsrFI RCCGGY 3 cut(s) 11, 114, 245
BsrI ACTGG 2 cut(s) 69, 223
BssAI RCCGGY 3 cut(s) 11, 114, 245
BssECI CCNNGG 4 cut(s) 205, 354, 393, 436
BssMI GATC 1 cut(s) 348
BssNI GRCGYC 3 cut(s) 42, 138, 491
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 3 cut(s) 355, 367, 395
Bst4CI ACNGT 4 cut(s) 166, 373, 427, 471
BstACI GRCGYC 3 cut(s) 42, 138, 491
BstC8I GCNNGC 2 cut(s) 171, 239
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 205
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 385
BstFNI CGCG 1 cut(s) 269
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 1 cut(s) 351
BstMBI GATC 1 cut(s) 348
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 179, 306
BstNI CCWGG 3 cut(s) 355, 367, 395
BstSCI CCNGG 5 cut(s) 353, 365, 393, 540, 559
BstSLI GKGCMC 1 cut(s) 171
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 236
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 3 cut(s) 15, 161, 309
BtgI CCRYGG 1 cut(s) 205
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 1 cut(s) 385
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 171, 239
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 3 cut(s) 11, 114, 245
Cfr13I GGNCC 4 cut(s) 209, 308, 369, 568
CseI GACGC 4 cut(s) 50, 146, 258, 275
Csp6I GTAC 3 cut(s) 359, 406, 508
CviAII CATG 1 cut(s) 206
CviJI RGCY 8 cut(s) 15, 58, 118, 161, 249, 309, 330, 534
CviKI_1 RGCY 8 cut(s) 15, 58, 118, 161, 249, 309, 330, 534
CviQI GTAC 3 cut(s) 359, 406, 508
DdeI CTNAG 1 cut(s) 288
DpnI GATC 1 cut(s) 350
DpnII GATC 1 cut(s) 348
EaeI YGGCCR 2 cut(s) 13, 159
EciI GGCGGA 2 cut(s) 201, 445
Ecl136II GAGCTC 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 536
Eco47I GGWCC 3 cut(s) 209, 369, 568
Eco53kI GAGCTC 1 cut(s) 534
Eco88I CYCGRG 2 cut(s) 191, 437
EcoICRI GAGCTC 1 cut(s) 534
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoRII CCWGG 3 cut(s) 353, 365, 393
EcoT14I CCWWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 1 cut(s) 209
FaiI YATR 2 cut(s) 135, 207
FatI CATG 1 cut(s) 205
FauI CCCGC 1 cut(s) 440
FblI GTMKAC 1 cut(s) 488
Fnu4HI GCNGC 2 cut(s) 183, 250
FokI GGATG 1 cut(s) 392
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 2 cut(s) 183, 250
FspBI CTAG 2 cut(s) 59, 320
GlaI GCGC 1 cut(s) 236
GluI GCNGC 2 cut(s) 183, 250
HaeIII GGCC 3 cut(s) 15, 161, 309
HapII CCGG 5 cut(s) 12, 115, 246, 541, 560
HgaI GACGC 4 cut(s) 50, 146, 258, 275
HhaI GCGC 1 cut(s) 237
Hin1I GRCGYC 3 cut(s) 42, 138, 491
Hin1II CATG 1 cut(s) 209
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 2 cut(s) 89, 101
HpaII CCGG 5 cut(s) 12, 115, 246, 541, 560
HphI GGTGA 4 cut(s) 104, 382, 415, 421
Hpy166II GTNNAC 3 cut(s) 169, 361, 489
Hpy188I TCNGA 3 cut(s) 100, 126, 496
Hpy188III TCNNGA 4 cut(s) 77, 229, 439, 547
Hpy8I GTNNAC 3 cut(s) 169, 361, 489
Hpy99I CGWCG 7 cut(s) 44, 89, 269, 274, 308, 317, 493
HpyCH4III ACNGT 4 cut(s) 166, 373, 427, 471
HpyCH4IV ACGT 2 cut(s) 156, 491
HpyCH4V TGCA 1 cut(s) 169
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 306
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 2 cut(s) 156, 491
Hsp92I GRCGYC 3 cut(s) 42, 138, 491
Hsp92II CATG 1 cut(s) 209
HspAI GCGC 1 cut(s) 235
Kzo9I GATC 1 cut(s) 348
LmnI GCTCC 2 cut(s) 305, 539
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 1 cut(s) 222
MaeI CTAG 2 cut(s) 59, 320
MaeII ACGT 2 cut(s) 156, 491
MaeIII GTNAC 4 cut(s) 152, 332, 373, 427
MalI GATC 1 cut(s) 350
MboI GATC 1 cut(s) 348
MboII GAAGA 2 cut(s) 84, 87
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 3 cut(s) 149, 171, 536
MlsI TGGCCA 1 cut(s) 161
MluI ACGCGT 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 561
MnlI CCTC 9 cut(s) 188, 200, 297, 319, 431, 446, 463, 529, 581
Mox20I TGGCCA 1 cut(s) 161
MscI TGGCCA 1 cut(s) 161
Msp20I TGGCCA 1 cut(s) 161
MspI CCGG 5 cut(s) 12, 115, 246, 541, 560
MspR9I CCNGG 5 cut(s) 355, 367, 395, 542, 561
MvaI CCWGG 3 cut(s) 355, 367, 395
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 179, 306
NciI CCSGG 2 cut(s) 542, 561
NcoI CCATGG 1 cut(s) 205
NdeII GATC 1 cut(s) 348
NlaIII CATG 1 cut(s) 209
NlaIV GGNNCC 3 cut(s) 203, 310, 558
NmuCI GTSAC 4 cut(s) 152, 332, 373, 427
PaeR7I CTCGAG 1 cut(s) 191
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 162, 312, 369
PfeI GAWTC 2 cut(s) 89, 101
PkrI GCNGC 2 cut(s) 184, 251
Psp124BI GAGCTC 1 cut(s) 536
Psp6I CCWGG 3 cut(s) 353, 365, 393
PspGI CCWGG 3 cut(s) 353, 365, 393
PspN4I GGNNCC 3 cut(s) 203, 310, 558
PspPI GGNCC 4 cut(s) 209, 308, 369, 568
PspXI VCTCGAGB 1 cut(s) 191
PsuI RGATCY 1 cut(s) 348
RsaI GTAC 3 cut(s) 360, 407, 509
RsaNI GTAC 3 cut(s) 359, 406, 508
SacI GAGCTC 1 cut(s) 536
SalI GTCGAC 1 cut(s) 487
SatI GCNGC 2 cut(s) 183, 250
Sau3AI GATC 1 cut(s) 348
Sau96I GGNCC 4 cut(s) 209, 308, 369, 568
ScaI AGTACT 1 cut(s) 407
ScrFI CCNGG 5 cut(s) 355, 367, 395, 542, 561
SduI GDGCHC 3 cut(s) 149, 171, 536
SetI ASST 7 cut(s) 60, 159, 192, 399, 455, 494, 536
SfaNI GCATC 1 cut(s) 222
Sfr274I CTCGAG 1 cut(s) 191
SinI GGWCC 3 cut(s) 209, 369, 568
SlaI CTCGAG 1 cut(s) 191
SmlI CTYRAG 3 cut(s) 75, 142, 191
SmoI CTYRAG 3 cut(s) 75, 142, 191
SsiI CCGC 4 cut(s) 183, 212, 433, 456
SspMI CTAG 2 cut(s) 59, 320
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 5 cut(s) 353, 365, 393, 540, 559
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 4 cut(s) 166, 373, 427, 471
TaiI ACGT 2 cut(s) 159, 494
TaqI TCGA 2 cut(s) 192, 488
TatI WGTACW 1 cut(s) 405
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 2 cut(s) 89, 101
TscAI CASTG 1 cut(s) 518
TseFI GTSAC 4 cut(s) 152, 332, 373, 427
TseI GCWGC 1 cut(s) 249
Tsp45I GTSAC 4 cut(s) 152, 332, 373, 427
TspGWI ACGGA 3 cut(s) 292, 392, 511
TspRI CASTG 1 cut(s) 518
VneI GTGCAC 1 cut(s) 167
VpaK11BI GGWCC 3 cut(s) 209, 369, 568
XagI CCTNNNNNAGG 1 cut(s) 323
XhoI CTCGAG 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 488
XspI CTAG 2 cut(s) 59, 320
ZraI GACGTC 1 cut(s) 492
ZrmI AGTACT 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.