Rw5G043820

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005419
Physical Location & Seq
Reverse (-)
76925362 .. 76925973
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043820.1

Sequence Viewer

Length: 612 bp
ATGCAACCGTCCGAATTCCACACATGGTCTCCTACTCTATATAAACCTCAAAACCATCCAGCTTCCACATTCAAAAGCCATCATTTCCAGAACTCAAAGCCTCAACAACTCAGAGCTCCAAAACTGGCTAACCCTCGAGAATGTGGCCTGGAGGGAGAAAAGAAAGGAGACTTGGAGTCTCGCAACAAGCGAGCCGTGACGGCTCTGTACGACGCCTTCAACTCCAAAGACGTTGACGTTGTTCACCTTCTCCTCGCGCCCTACCTAGAGTGGTGGTTCCATGGCCCCCCTACTCATCAGCATTTGAACCGCCTCCTCACCGGTGCGCCGCCGTACGACTCGTCATTCATATTCCTTCCTCTGTCGATTGTTGCATTCGGATCAATGGTGGTGGCTGAGGGATACGAGGAAGGTCATTCGGTGTCGTGGGTGCACGCGTGGACCGTCACAGATGGGATAATTACCCAAGTGAGAGAGTACTACAACACGTCCGTCACGGTTACCAGGTTAATCTCACCGGAAATTACATCACGGTCAGGTAGTTGCCAGAGTGTTTGGGAGAGTAAGCTCTCTGATAATAAGTCTGTACCTGGCCTCGTATTGGCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WI12

Protein Analysis

203

Amino Acids

22.81

Weight (kDa)

6.79

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 104 - 203 1e-37 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 257, 437
AciI CCGC 2 cut(s) 310, 329
AclWI GGATC 1 cut(s) 388
AcsI RAATTY 1 cut(s) 14
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 4 cut(s) 209, 335, 479, 588
AfiI CCNNNNNNNGG 1 cut(s) 601
AflIII ACRYGT 2 cut(s) 435, 486
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 3 cut(s) 73, 220, 307
AhdI GACNNNNNGTC 1 cut(s) 175
AjiI CACGTC 1 cut(s) 489
AjnI CCWGG 3 cut(s) 147, 503, 589
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AluBI AGCT 3 cut(s) 62, 116, 568
AluI AGCT 3 cut(s) 62, 116, 568
Alw21I GWGCWC 2 cut(s) 118, 435
Alw26I GTCTC 3 cut(s) 33, 162, 183
Alw44I GTGCAC 1 cut(s) 431
AlwI GGATC 1 cut(s) 388
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 4 cut(s) 145, 283, 592, 603
ApaLI GTGCAC 1 cut(s) 431
ApoI RAATTY 1 cut(s) 14
AsiGI ACCGGT 1 cut(s) 320
AspLEI GCGC 2 cut(s) 259, 328
AspS9I GGNCC 3 cut(s) 284, 441, 604
AsuHPI GGTGA 3 cut(s) 236, 310, 507
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 435
BanII GRGCYC 1 cut(s) 118
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
Bbv12I GWGCWC 2 cut(s) 118, 435
BbvCI CCTCAGC 1 cut(s) 396
BccI CCATC 3 cut(s) 63, 87, 446
BceAI ACGGC 3 cut(s) 179, 216, 316
BciT130I CCWGG 3 cut(s) 149, 505, 591
BciVI GTATCC 1 cut(s) 395
BcoDI GTCTC 3 cut(s) 33, 162, 183
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 608
BfuI GTATCC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 1 cut(s) 329
BlsI GCNGC 1 cut(s) 330
BmcAI AGTACT 1 cut(s) 479
Bme1390I CCNGG 3 cut(s) 149, 505, 591
Bme18I GGWCC 1 cut(s) 441
BmeRI GACNNNNNGTC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 489
BmgT120I GGNCC 3 cut(s) 284, 441, 604
BmiI GGNNCC 2 cut(s) 278, 286
BmrFI CCNGG 3 cut(s) 149, 505, 591
BpmI CTGGAG 1 cut(s) 170
Bpu10I CCTNAGC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 2 cut(s) 320, 517
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 601
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 129
BseBI CCWGG 3 cut(s) 149, 505, 591
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 601
BseMII CTCAG 2 cut(s) 124, 387
BseNI ACTGG 1 cut(s) 129
BseRI GAGGAG 2 cut(s) 242, 305
BseSI GKGCMC 1 cut(s) 435
Bsh1236I CGCG 2 cut(s) 257, 437
BshFI GGCC 4 cut(s) 147, 285, 594, 605
BshTI ACCGGT 1 cut(s) 320
BsiHKAI GWGCWC 2 cut(s) 118, 435
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 2 cut(s) 321, 518
BsiWI CGTACG 1 cut(s) 333
BslI CCNNNNNNNGG 1 cut(s) 601
BsmAI GTCTC 3 cut(s) 33, 162, 183
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 4 cut(s) 147, 285, 594, 605
Bso31I GGTCTC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 118, 435
Bsp143I GATC 1 cut(s) 380
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 2 cut(s) 310, 329
BspANI GGCC 4 cut(s) 147, 285, 594, 605
BspCNI CTCAG 2 cut(s) 123, 388
BspFNI CGCG 2 cut(s) 257, 437
BspLI GGNNCC 2 cut(s) 278, 286
BspPI GGATC 1 cut(s) 388
BspTNI GGTCTC 1 cut(s) 33
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 1 cut(s) 380
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 280
Bst2UI CCWGG 3 cut(s) 149, 505, 591
Bst4CI ACNGT 4 cut(s) 9, 445, 499, 534
BstACI GRCGYC 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 192, 435
BstDEI CTNAG 2 cut(s) 110, 396
BstDSI CCRYGG 1 cut(s) 280
BstEII GGTNACC 1 cut(s) 499
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 2 cut(s) 257, 437
BstHHI GCGC 2 cut(s) 259, 328
BstKTI GATC 1 cut(s) 383
BstMAI GTCTC 3 cut(s) 33, 162, 183
BstMBI GATC 1 cut(s) 380
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 3 cut(s) 149, 505, 591
BstPI GGTNACC 1 cut(s) 499
BstSCI CCNGG 3 cut(s) 147, 503, 589
BstSFI CTRYAG 1 cut(s) 608
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 2 cut(s) 257, 437
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 4 cut(s) 147, 285, 594, 605
BtgI CCRYGG 1 cut(s) 280
BtrI CACGTC 1 cut(s) 489
BtsCI GGATG 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 192, 435
CfoI GCGC 2 cut(s) 259, 328
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 284, 441, 604
CseI GACGC 1 cut(s) 221
CsiI ACCWGGT 1 cut(s) 503
Csp6I GTAC 4 cut(s) 208, 334, 478, 587
CspAI ACCGGT 1 cut(s) 320
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 2 cut(s) 24, 281
CviQI GTAC 4 cut(s) 208, 334, 478, 587
DdeI CTNAG 2 cut(s) 110, 396
DpnI GATC 1 cut(s) 382
DpnII GATC 1 cut(s) 380
DriI GACNNNNNGTC 1 cut(s) 175
Eam1105I GACNNNNNGTC 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 280
Eco24I GRGCYC 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 135
Eco91I GGTNACC 1 cut(s) 499
EcoICRI GAGCTC 1 cut(s) 116
EcoO65I GGTNACC 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 3 cut(s) 147, 503, 589
EcoT14I CCWWGG 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 2 cut(s) 27, 284
FaiI YATR 6 cut(s) 25, 40, 42, 282, 350, 610
FatI CATG 2 cut(s) 23, 280
Fnu4HI GCNGC 1 cut(s) 329
FokI GGATG 1 cut(s) 42
FriOI GRGCYC 1 cut(s) 118
Fsp4HI GCNGC 1 cut(s) 329
FspBI CTAG 1 cut(s) 266
GlaI GCGC 2 cut(s) 258, 327
GluI GCNGC 1 cut(s) 329
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 4 cut(s) 147, 285, 594, 605
HapII CCGG 2 cut(s) 321, 518
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 259, 328
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 2 cut(s) 27, 284
Hin6I GCGC 2 cut(s) 257, 326
HinP1I GCGC 2 cut(s) 257, 326
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 2 cut(s) 176, 338
HpaII CCGG 2 cut(s) 321, 518
HphI GGTGA 3 cut(s) 236, 310, 507
Hpy166II GTNNAC 4 cut(s) 235, 244, 433, 441
Hpy188I TCNGA 4 cut(s) 13, 113, 380, 574
Hpy188III TCNNGA 2 cut(s) 88, 137
Hpy8I GTNNAC 4 cut(s) 235, 244, 433, 441
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 226, 257, 365, 404
HpyCH4III ACNGT 4 cut(s) 9, 445, 499, 534
HpyCH4IV ACGT 3 cut(s) 231, 237, 488
HpyCH4V TGCA 3 cut(s) 4, 374, 433
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 2 cut(s) 110, 396
HpySE526I ACGT 3 cut(s) 231, 237, 488
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 2 cut(s) 27, 284
HspAI GCGC 2 cut(s) 257, 326
Kzo9I GATC 1 cut(s) 380
LmnI GCTCC 1 cut(s) 121
MabI ACCWGGT 1 cut(s) 503
MaeI CTAG 1 cut(s) 266
MaeII ACGT 3 cut(s) 231, 237, 488
MaeIII GTNAC 4 cut(s) 196, 445, 493, 499
MalI GATC 1 cut(s) 382
MboI GATC 1 cut(s) 380
MhlI GDGCHC 2 cut(s) 118, 435
MluCI AATT 3 cut(s) 14, 459, 522
MluI ACGCGT 1 cut(s) 435
MlyI GAGTC 2 cut(s) 185, 332
MseI TTAA 1 cut(s) 509
MspI CCGG 2 cut(s) 321, 518
MspR9I CCNGG 3 cut(s) 149, 505, 591
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 3 cut(s) 149, 505, 591
MvnI CGCG 2 cut(s) 257, 437
MwoI GCNNNNNNNGC 1 cut(s) 200
NcoI CCATGG 1 cut(s) 280
NdeII GATC 1 cut(s) 380
NlaIII CATG 2 cut(s) 27, 284
NlaIV GGNNCC 2 cut(s) 278, 286
NmuCI GTSAC 3 cut(s) 196, 445, 493
PaeR7I CTCGAG 1 cut(s) 135
PcsI WCGNNNNNNNCGW 2 cut(s) 187, 441
PctI GAATGC 1 cut(s) 374
Pfl23II CGTACG 1 cut(s) 333
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 1 cut(s) 330
PleI GAGTC 2 cut(s) 184, 332
PpsI GAGTC 2 cut(s) 184, 332
Psp124BI GAGCTC 1 cut(s) 118
Psp6I CCWGG 3 cut(s) 147, 503, 589
PspEI GGTNACC 1 cut(s) 499
PspGI CCWGG 3 cut(s) 147, 503, 589
PspLI CGTACG 1 cut(s) 333
PspN4I GGNNCC 2 cut(s) 278, 286
PspPI GGNCC 3 cut(s) 284, 441, 604
RsaI GTAC 4 cut(s) 209, 335, 479, 588
RsaNI GTAC 4 cut(s) 208, 334, 478, 587
SacI GAGCTC 1 cut(s) 118
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 1 cut(s) 329
Sau3AI GATC 1 cut(s) 380
Sau96I GGNCC 3 cut(s) 284, 441, 604
ScaI AGTACT 1 cut(s) 479
SchI GAGTC 2 cut(s) 185, 332
ScrFI CCNGG 3 cut(s) 149, 505, 591
SduI GDGCHC 2 cut(s) 118, 435
SexAI ACCWGGT 1 cut(s) 503
SfcI CTRYAG 1 cut(s) 608
Sfr274I CTCGAG 1 cut(s) 135
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 1 cut(s) 441
SlaI CTCGAG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
Sse9I AATT 3 cut(s) 14, 459, 522
SsiI CCGC 2 cut(s) 310, 329
SspMI CTAG 1 cut(s) 266
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 3 cut(s) 147, 503, 589
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 4 cut(s) 9, 445, 499, 534
TaiI ACGT 3 cut(s) 234, 240, 491
TaqI TCGA 2 cut(s) 136, 365
TasI AATT 3 cut(s) 14, 459, 522
TatI WGTACW 1 cut(s) 477
TauI GCSGC 1 cut(s) 331
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TseFI GTSAC 3 cut(s) 196, 445, 493
Tsp45I GTSAC 3 cut(s) 196, 445, 493
TspDTI ATGAA 1 cut(s) 337
TspGWI ACGGA 1 cut(s) 481
VneI GTGCAC 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 441
XapI RAATTY 1 cut(s) 14
XhoI CTCGAG 1 cut(s) 135
XspI CTAG 1 cut(s) 266
ZrmI AGTACT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.