MD03G1262300.v1.1

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002798
Physical Location & Seq
Forward (+)
34821354 .. 34821842
489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1262300.v1.1.491

Sequence Viewer

Length: 489 bp
ATGAAGAAGAAAGTCCACCCTTCGTCCCAAAAGCGTAATACGTTATACGACGTCGTGACATCCTCTTCCACGCCGCCAAAGAAGCTCGGAAGAAGACTCCCCCACCTGTTCGCCAGCGTCCTCGAGCTCCCCTTCCACTCAAACGCCGACGTTTCCATCCAAAAAACCTCTGACTCTTTCATTTTCTCCGTCTCCATGCCCACGCATGTGCCGTCGCCCGCCACCCAACGAATAACTGGCGACCACGTTGCTGTACGGGCTCACACGATAGAGATTTATCCCGGAGTGACAAAGACTGTGATAAGGAAAACGGACGGTAGTGATTTGTGGTGGTTGGACGGAGGAGTAGTGGACGATCTTGATCTCTGGAGGTATCGGCTGCCGGCTTCGACGAGACCGGACTTGGCCAGGACTACGTGTACTGGGGAAGAGCTGGTTGTAACAGTTCCGACGAAGAGGAGGTTTTGGGTGAAGAAAATGGGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.15

Weight (kDa)

9.94

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
AciI CCGC 2 cut(s) 74, 219
AcoI YGGCCR 1 cut(s) 405
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 255, 421
AflIII ACRYGT 1 cut(s) 416
AjnI CCWGG 1 cut(s) 407
AleI CACNNNNGTG 1 cut(s) 206
AluBI AGCT 3 cut(s) 85, 127, 433
AluI AGCT 3 cut(s) 85, 127, 433
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 196, 388
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 1 cut(s) 405
ApeKI GCWGC 1 cut(s) 379
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 122
BalI TGGCCA 1 cut(s) 407
BanII GRGCYC 2 cut(s) 129, 262
BbsI GAAGAC 1 cut(s) 100
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 366
BccI CCATC 1 cut(s) 164
BceAI ACGGC 1 cut(s) 196
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 1 cut(s) 409
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 196, 388
BisI GCNGC 2 cut(s) 74, 380
BlsI GCNGC 2 cut(s) 75, 381
Bme1390I CCNGG 2 cut(s) 282, 409
BmeT110I CYCGRG 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 282, 409
BmrI ACTGGG 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 432
BpiI GAAGAC 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 388
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 397
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 2 cut(s) 241, 427
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 1 cut(s) 409
BseGI GGATG 2 cut(s) 59, 156
BseJI GATNNNNATC 1 cut(s) 360
BseNI ACTGG 2 cut(s) 241, 427
BseRI GAGGAG 2 cut(s) 357, 472
BseXI GCAGC 1 cut(s) 366
BshFI GGCC 1 cut(s) 407
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 3 cut(s) 282, 383, 398
BslFI GGGAC 1 cut(s) 10
BsmAI GTCTC 2 cut(s) 196, 388
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 1 cut(s) 407
Bso31I GGTCTC 1 cut(s) 388
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 129, 262
Bsp143I GATC 2 cut(s) 355, 361
BspACI CCGC 2 cut(s) 74, 219
BspANI GGCC 1 cut(s) 407
BspQI GCTCTTC 1 cut(s) 423
BspTNI GGTCTC 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 2 cut(s) 241, 427
BssAI RCCGGY 1 cut(s) 382
BssMI GATC 2 cut(s) 355, 361
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 409
Bst4CI ACNGT 3 cut(s) 298, 317, 445
Bst6I CTCTTC 3 cut(s) 70, 423, 449
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 417
BstC8I GCNNGC 3 cut(s) 115, 219, 384
BstF5I GGATG 2 cut(s) 59, 156
BstKTI GATC 2 cut(s) 358, 364
BstMAI GTCTC 2 cut(s) 196, 388
BstMBI GATC 2 cut(s) 355, 361
BstMWI GCNNNNNNNGC 2 cut(s) 82, 257
BstNI CCWGG 1 cut(s) 409
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 280, 407
BstV1I GCAGC 1 cut(s) 366
BstV2I GAAGAC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 407
BtsCI GGATG 2 cut(s) 59, 156
Cac8I GCNNGC 3 cut(s) 115, 219, 384
Cfr10I RCCGGY 1 cut(s) 382
CseI GACGC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 254, 420
CviAII CATG 2 cut(s) 196, 206
CviJI RGCY 8 cut(s) 85, 127, 260, 379, 386, 407, 433, 486
CviKI_1 RGCY 8 cut(s) 85, 127, 260, 379, 386, 407, 433, 486
CviQI GTAC 2 cut(s) 254, 420
DpnI GATC 2 cut(s) 357, 363
DpnII GATC 2 cut(s) 355, 361
EaeI YGGCCR 1 cut(s) 405
Eam1104I CTCTTC 3 cut(s) 70, 423, 449
EarI CTCTTC 3 cut(s) 70, 423, 449
Ecl136II GAGCTC 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 129, 262
Eco31I GGTCTC 1 cut(s) 388
Eco53kI GAGCTC 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 122
EcoICRI GAGCTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 407
EcoT38I GRGCYC 2 cut(s) 129, 262
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 2 cut(s) 199, 209
FaiI YATR 3 cut(s) 46, 197, 207
FaqI GGGAC 1 cut(s) 10
FatI CATG 2 cut(s) 195, 205
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 2 cut(s) 74, 380
FokI GGATG 2 cut(s) 46, 143
FriOI GRGCYC 2 cut(s) 129, 262
Fsp4HI GCNGC 2 cut(s) 74, 380
GluI GCNGC 2 cut(s) 74, 380
GsuI CTGGAG 1 cut(s) 388
HaeIII GGCC 1 cut(s) 407
HapII CCGG 3 cut(s) 282, 383, 398
HgaI GACGC 1 cut(s) 106
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 2 cut(s) 199, 209
HinfI GANTC 2 cut(s) 96, 173
HpaII CCGG 3 cut(s) 282, 383, 398
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 3 cut(s) 16, 352, 420
Hpy188I TCNGA 3 cut(s) 89, 172, 450
Hpy188III TCNNGA 3 cut(s) 55, 359, 367
Hpy8I GTNNAC 3 cut(s) 16, 352, 420
Hpy99I CGWCG 6 cut(s) 53, 56, 152, 217, 394, 454
HpyAV CCTTC 2 cut(s) 30, 142
HpyCH4III ACNGT 3 cut(s) 298, 317, 445
HpyCH4IV ACGT 5 cut(s) 41, 51, 150, 246, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 257
HpySE526I ACGT 5 cut(s) 41, 51, 150, 246, 416
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 2 cut(s) 199, 209
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Kzo9I GATC 2 cut(s) 355, 361
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 132
Lsp1109I GCAGC 1 cut(s) 366
MaeII ACGT 5 cut(s) 41, 51, 150, 246, 416
MaeIII GTNAC 3 cut(s) 55, 286, 439
MalI GATC 2 cut(s) 357, 363
MboI GATC 2 cut(s) 355, 361
MboII GAAGA 8 cut(s) 16, 19, 57, 102, 105, 440, 466, 484
MhlI GDGCHC 2 cut(s) 129, 262
MlsI TGGCCA 1 cut(s) 407
MluNI TGGCCA 1 cut(s) 407
MlyI GAGTC 2 cut(s) 90, 167
MmeI TCCRAC 2 cut(s) 315, 473
MnlI CCTC 8 cut(s) 73, 131, 178, 335, 363, 450, 453, 476
Mox20I TGGCCA 1 cut(s) 407
MroNI GCCGGC 1 cut(s) 382
MscI TGGCCA 1 cut(s) 407
MslI CAYNNNNRTG 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 407
MspI CCGG 3 cut(s) 282, 383, 398
MspR9I CCNGG 2 cut(s) 282, 409
MvaI CCWGG 1 cut(s) 409
MwoI GCNNNNNNNGC 2 cut(s) 82, 257
NaeI GCCGGC 1 cut(s) 384
NciI CCSGG 1 cut(s) 282
NdeII GATC 2 cut(s) 355, 361
NgoMIV GCCGGC 1 cut(s) 382
NlaIII CATG 2 cut(s) 199, 209
NmuCI GTSAC 2 cut(s) 55, 286
NspI RCATGY 1 cut(s) 209
OliI CACNNNNGTG 1 cut(s) 206
PaeR7I CTCGAG 1 cut(s) 122
PciSI GCTCTTC 1 cut(s) 423
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PdiI GCCGGC 1 cut(s) 384
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 2 cut(s) 75, 381
PleI GAGTC 2 cut(s) 90, 167
PpsI GAGTC 2 cut(s) 90, 167
Ppu21I YACGTR 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 407
PspGI CCWGG 1 cut(s) 407
PspXI VCTCGAGB 1 cut(s) 122
RsaI GTAC 2 cut(s) 255, 421
RsaNI GTAC 2 cut(s) 254, 420
RseI CAYNNNNRTG 1 cut(s) 206
SacI GAGCTC 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 423
SatI GCNGC 2 cut(s) 74, 380
Sau3AI GATC 2 cut(s) 355, 361
SchI GAGTC 2 cut(s) 90, 167
ScrFI CCNGG 2 cut(s) 282, 409
SduI GDGCHC 2 cut(s) 129, 262
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SsiI CCGC 2 cut(s) 74, 219
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 2 cut(s) 280, 407
TaaI ACNGT 3 cut(s) 298, 317, 445
TaiI ACGT 5 cut(s) 44, 54, 153, 249, 419
TaqI TCGA 2 cut(s) 123, 389
TatI WGTACW 1 cut(s) 419
TauI GCSGC 1 cut(s) 76
TseFI GTSAC 2 cut(s) 55, 286
TseI GCWGC 1 cut(s) 379
Tsp45I GTSAC 2 cut(s) 55, 286
TspDTI ATGAA 2 cut(s) 17, 169
TspGWI ACGGA 3 cut(s) 178, 326, 354
XceI RCATGY 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XhoI CTCGAG 1 cut(s) 122
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.