MD11G1283000.v1.1

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002798
Physical Location & Seq
Forward (+)
40136823 .. 40137371
549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1283000.v1.1.491

Sequence Viewer

Length: 549 bp
ATGAAGAAAAAAGTCTACCCCTCCCCCAAAAAGCGCAACTTGTTATACGACGTCGTCGCATCATCTTCTTCCATGCCGCCGAAGAAGCTCCGGAGACTCCCCCACGTGTTCGCCAGCGTTCTCGAGCTCCCCTTCCACTCAAACGCCGACGTTTCCGTCCAAGAAACCTCCGACTCTTTCATTTTCTCAGTCGCCATGCCCGTGCATGTGACCACACCCACCACCGCGCGACCAGTTATTGGCGACCGCGTTGCTGTCCAAGCCCACACGATAGAGATTTATCCCGGGGTGACAAAGACTGTGATAAGGAAAACGGAAGGCGGTGATTTGTGGAGGTTGGACGAAGGAGTAGTAGACGATCTTGATCTCTGGAGGTATCGGCTGCCGGCATCGACGAGACCGGAGTTGGCCAGGGCCACGTGTACTAGGGAAGAACTGGTTGTGACAGTTCCAAAGGACCATGTTCATGATCGTTATGATCGTAATAAGCAAGGTGTAGAAGAAGAGGTTTTGGGTGAAGAAAATGGGAGGCATATTATTGTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.58

Weight (kDa)

7.9

Isoelectric Point (pI)

60.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 155
AatII GACGTC 1 cut(s) 54
AccB7I CCANNNNNTGG 1 cut(s) 239
AccI GTMKAC 2 cut(s) 15, 354
AccII CGCG 3 cut(s) 227, 229, 249
AccIII TCCGGA 1 cut(s) 90
AciI CCGC 4 cut(s) 77, 225, 247, 321
AcoI YGGCCR 1 cut(s) 408
AcvI CACGTG 2 cut(s) 106, 420
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 424, 543
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 2 cut(s) 105, 419
AjnI CCWGG 1 cut(s) 410
AluBI AGCT 2 cut(s) 88, 127
AluI AGCT 2 cut(s) 88, 127
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 88, 391
Ama87I CYCGRG 2 cut(s) 122, 284
Aor13HI TCCGGA 1 cut(s) 90
AoxI GGCC 2 cut(s) 408, 414
ApeKI GCWGC 1 cut(s) 382
AspLEI GCGC 2 cut(s) 36, 229
AspS9I GGNCC 2 cut(s) 414, 457
AsuC2I CCSGG 2 cut(s) 285, 286
AsuHPI GGTGA 3 cut(s) 301, 335, 527
AvaI CYCGRG 2 cut(s) 122, 284
AvaII GGWCC 1 cut(s) 457
BalI TGGCCA 1 cut(s) 410
BanII GRGCYC 1 cut(s) 129
BbrPI CACGTG 2 cut(s) 106, 420
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 369
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 1 cut(s) 412
BcnI CCSGG 2 cut(s) 285, 286
BcoDI GTCTC 2 cut(s) 88, 391
BfaI CTAG 1 cut(s) 426
BisI GCNGC 2 cut(s) 77, 383
BlsI GCNGC 2 cut(s) 78, 384
Bme1390I CCNGG 3 cut(s) 285, 286, 412
Bme18I GGWCC 1 cut(s) 457
BmeT110I CYCGRG 2 cut(s) 122, 284
BmgT120I GGNCC 2 cut(s) 414, 457
BmrFI CCNGG 3 cut(s) 285, 286, 412
BmsI GCATC 2 cut(s) 68, 398
BpmI CTGGAG 1 cut(s) 391
BpuMI CCSGG 2 cut(s) 285, 286
BsaAI YACGTR 2 cut(s) 106, 420
BsaBI GATNNNNATC 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 3 cut(s) 284, 285, 411
BsaWI WCCGGW 2 cut(s) 90, 400
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 2 cut(s) 233, 441
Bse8I GATNNNNATC 1 cut(s) 363
BseAI TCCGGA 1 cut(s) 90
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 284, 285, 411
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 2 cut(s) 233, 441
BseXI GCAGC 1 cut(s) 369
Bsh1236I CGCG 3 cut(s) 227, 229, 249
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 2 cut(s) 410, 416
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 2 cut(s) 122, 284
BsiSI CCGG 4 cut(s) 91, 285, 386, 401
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 88, 391
BsnI GGCC 2 cut(s) 410, 416
Bso31I GGTCTC 1 cut(s) 391
BsoBI CYCGRG 2 cut(s) 122, 284
Bsp1286I GDGCHC 1 cut(s) 129
Bsp13I TCCGGA 1 cut(s) 90
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 4 cut(s) 358, 364, 469, 478
BspACI CCGC 4 cut(s) 77, 225, 247, 321
BspANI GGCC 2 cut(s) 410, 416
BspCNI CTCAG 1 cut(s) 200
BspEI TCCGGA 1 cut(s) 90
BspFNI CGCG 3 cut(s) 227, 229, 249
BspHI TCATGA 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 385
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 2 cut(s) 233, 441
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 284, 285, 411
BssMI GATC 4 cut(s) 358, 364, 469, 478
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 301, 448
Bst6I CTCTTC 1 cut(s) 498
BstACI GRCGYC 1 cut(s) 51
BstAUI TGTACA 1 cut(s) 541
BstBAI YACGTR 2 cut(s) 106, 420
BstC8I GCNNGC 2 cut(s) 115, 387
BstDEI CTNAG 1 cut(s) 187
BstFNI CGCG 3 cut(s) 227, 229, 249
BstHHI GCGC 2 cut(s) 36, 229
BstKTI GATC 4 cut(s) 361, 367, 472, 481
BstMAI GTCTC 2 cut(s) 88, 391
BstMBI GATC 4 cut(s) 358, 364, 469, 478
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 2 cut(s) 85, 260
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 3 cut(s) 283, 284, 410
BstUI CGCG 3 cut(s) 227, 229, 249
BstV1I GCAGC 1 cut(s) 369
BsuRI GGCC 2 cut(s) 410, 416
Cac8I GCNNGC 2 cut(s) 115, 387
CciI TCATGA 1 cut(s) 466
CfoI GCGC 2 cut(s) 36, 229
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 414, 457
Cfr9I CCCGGG 1 cut(s) 284
Csp6I GTAC 2 cut(s) 423, 542
CviAII CATG 5 cut(s) 73, 196, 206, 461, 467
CviJI RGCY 6 cut(s) 88, 127, 263, 382, 410, 416
CviKI_1 RGCY 6 cut(s) 88, 127, 263, 382, 410, 416
CviQI GTAC 2 cut(s) 423, 542
DdeI CTNAG 1 cut(s) 187
DpnI GATC 4 cut(s) 360, 366, 471, 480
DpnII GATC 4 cut(s) 358, 364, 469, 478
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Ecl136II GAGCTC 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 457
Eco53kI GAGCTC 1 cut(s) 127
Eco72I CACGTG 2 cut(s) 106, 420
Eco88I CYCGRG 2 cut(s) 122, 284
EcoICRI GAGCTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 410
EcoT38I GRGCYC 1 cut(s) 129
FaeI CATG 5 cut(s) 76, 199, 209, 464, 470
FaiI YATR 8 cut(s) 46, 74, 197, 207, 462, 468, 477, 534
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FatI CATG 5 cut(s) 72, 195, 205, 460, 466
FblI GTMKAC 2 cut(s) 15, 354
Fnu4HI GCNGC 2 cut(s) 77, 383
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 2 cut(s) 77, 383
FspBI CTAG 1 cut(s) 426
GlaI GCGC 2 cut(s) 35, 228
GluI GCNGC 2 cut(s) 77, 383
GsuI CTGGAG 1 cut(s) 391
HaeIII GGCC 2 cut(s) 410, 416
HapII CCGG 4 cut(s) 91, 285, 386, 401
HhaI GCGC 2 cut(s) 36, 229
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 5 cut(s) 76, 199, 209, 464, 470
Hin6I GCGC 2 cut(s) 34, 227
HinP1I GCGC 2 cut(s) 34, 227
HinfI GANTC 2 cut(s) 96, 173
HpaII CCGG 4 cut(s) 91, 285, 386, 401
HphI GGTGA 3 cut(s) 301, 335, 527
Hpy166II GTNNAC 3 cut(s) 16, 355, 423
Hpy188I TCNGA 1 cut(s) 172
Hpy188III TCNNGA 5 cut(s) 91, 122, 362, 370, 467
Hpy8I GTNNAC 3 cut(s) 16, 355, 423
Hpy99I CGWCG 5 cut(s) 53, 56, 59, 152, 397
HpyAV CCTTC 3 cut(s) 142, 311, 338
HpyCH4III ACNGT 2 cut(s) 301, 448
HpyCH4IV ACGT 4 cut(s) 51, 105, 150, 419
HpyCH4V TGCA 1 cut(s) 205
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 260
HpyF3I CTNAG 1 cut(s) 187
HpySE526I ACGT 4 cut(s) 51, 105, 150, 419
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 5 cut(s) 76, 199, 209, 464, 470
HspAI GCGC 2 cut(s) 34, 227
Kpn2I TCCGGA 1 cut(s) 90
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 4 cut(s) 358, 364, 469, 478
LmnI GCTCC 2 cut(s) 93, 132
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 2 cut(s) 68, 398
MaeI CTAG 1 cut(s) 426
MaeII ACGT 4 cut(s) 51, 105, 150, 419
MaeIII GTNAC 3 cut(s) 208, 289, 442
MalI GATC 4 cut(s) 360, 366, 471, 480
MboI GATC 4 cut(s) 358, 364, 469, 478
MboII GAAGA 8 cut(s) 16, 57, 60, 94, 443, 512, 515, 530
MhlI GDGCHC 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 410
MluNI TGGCCA 1 cut(s) 410
MlyI GAGTC 2 cut(s) 90, 167
MmeI TCCRAC 2 cut(s) 195, 318
MnlI CCTC 6 cut(s) 31, 178, 327, 366, 499, 522
Mox20I TGGCCA 1 cut(s) 410
MroI TCCGGA 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 410
MslI CAYNNNNRTG 2 cut(s) 200, 465
Msp20I TGGCCA 1 cut(s) 410
MspI CCGG 4 cut(s) 91, 285, 386, 401
MspR9I CCNGG 3 cut(s) 285, 286, 412
MvaI CCWGG 1 cut(s) 412
MvnI CGCG 3 cut(s) 227, 229, 249
MwoI GCNNNNNNNGC 2 cut(s) 85, 260
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 2 cut(s) 285, 286
NdeII GATC 4 cut(s) 358, 364, 469, 478
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 5 cut(s) 76, 199, 209, 464, 470
NmuCI GTSAC 3 cut(s) 208, 289, 442
NspI RCATGY 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 1 cut(s) 466
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 478
PdiI GCCGGC 1 cut(s) 387
PflFI GACNNNGTC 1 cut(s) 53
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 2 cut(s) 78, 384
PleI GAGTC 2 cut(s) 90, 167
PmaCI CACGTG 2 cut(s) 106, 420
PmlI CACGTG 2 cut(s) 106, 420
PpsI GAGTC 2 cut(s) 90, 167
Ppu21I YACGTR 2 cut(s) 106, 420
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 410
PspCI CACGTG 2 cut(s) 106, 420
PspGI CCWGG 1 cut(s) 410
PspPI GGNCC 2 cut(s) 414, 457
PsyI GACNNNGTC 1 cut(s) 53
RsaI GTAC 2 cut(s) 424, 543
RsaNI GTAC 2 cut(s) 423, 542
RseI CAYNNNNRTG 2 cut(s) 200, 465
SacI GAGCTC 1 cut(s) 129
SatI GCNGC 2 cut(s) 77, 383
Sau3AI GATC 4 cut(s) 358, 364, 469, 478
Sau96I GGNCC 2 cut(s) 414, 457
SchI GAGTC 2 cut(s) 90, 167
ScrFI CCNGG 3 cut(s) 285, 286, 412
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 2 cut(s) 68, 398
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 457
SlaI CTCGAG 1 cut(s) 122
SmaI CCCGGG 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 200, 465
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SsiI CCGC 4 cut(s) 77, 225, 247, 321
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 283, 284, 410
TaaI ACNGT 2 cut(s) 301, 448
TaiI ACGT 4 cut(s) 54, 108, 153, 422
TaqI TCGA 2 cut(s) 123, 392
TatI WGTACW 2 cut(s) 422, 541
TauI GCSGC 1 cut(s) 79
TseFI GTSAC 3 cut(s) 208, 289, 442
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 3 cut(s) 208, 289, 442
TspDTI ATGAA 3 cut(s) 17, 169, 455
TspGWI ACGGA 2 cut(s) 145, 329
TspMI CCCGGG 1 cut(s) 284
Tth111I GACNNNGTC 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 457
XceI RCATGY 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 122
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 2 cut(s) 15, 354
XspI CTAG 1 cut(s) 426
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.