MD04G1135200.v1.1

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001690
Physical Location & Seq
Forward (+)
22231346 .. 22234376
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1135200.v1.1.491

Sequence Viewer

Length: 783 bp
ATGGCAGGTTTCGACAGCCAAAGATGGTCTCTCAAGGGCATGACTGCCCTCGTCACCGGTGGAACAAAAGGCATCGGATATGCCATTGTGGAGGAACTAGCAGGACTTGGAGCAACCGTGCACACCTGCGCTCGCAACCAGGCACAGATTCTTGAGAGGCTTCAAGAATGGGAAAGTAAGGGATTCAAAGTGACTGGCTCGGTTTGTGACTTAACCTCTAAAGCACAAAGGGAGAACCTCATCAAAACTGTCTCCTCTATTTTTGAAGGCAAACTTAACATCCTTGTAAATAGTGCTGCAACTTGCACACTTAGAGGAACTACAGATTATACTTTGGAAGATTTCTCAAGTATGATGAAAACCAATGTTGAATCTCCATACCATCTTTCTCAACTTGCACATCCTCTCTTGAAGGCCTCAGGAAATGCAAGTATTGTCTTTGTTGCCTCCATTGCCGGTGTGGTAGCTCTACCGAAACTGTCTGCCTATGCAGCAACAAAAAGCGCAGTCATCCAAATTTCGAAGAACTTGGCATGCGAATGGGCAAAAGACAACATTCGTACTAATGCTGTGGCACCATGGGCTGTCAACACCGGAGTTAAAGTTGAATCTGATGGTCATTCTGATGATTTCAGGCGCCTAATAGGTCGAACTCCCATCCAACGCCTTGCACAGCCTAATGAAATATCATCTCTGATCACTTTCCTCTGCCTTCCTGCTGCTTCTTACATCAATGGACAAGTTATTAGTGTTGATGGAGGATTTACAGTTAGCGGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

261

Amino Acids

27.77

Weight (kDa)

8.57

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDR PF23441 13 - 212 3.2e-07 SDR-like rossmann domain
adh_short PF00106 15 - 203 5.5e-47 short chain dehydrogenase
KR PF08659 15 - 169 6.1e-14 KR domain
adh_short_C2 PF13561 23 - 256 2.7e-54 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 134
AccB1I GGYRCC 2 cut(s) 574, 636
AciI CCGC 1 cut(s) 774
AcsI RAATTY 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 637
AfaI GTAC 1 cut(s) 562
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 6 cut(s) 164, 187, 266, 371, 412, 608
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 1 cut(s) 467
AluI AGCT 1 cut(s) 467
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 33, 256
Alw44I GTGCAC 1 cut(s) 119
AoxI GGCC 1 cut(s) 414
ApaLI GTGCAC 1 cut(s) 119
ApeKI GCWGC 3 cut(s) 296, 491, 719
ApoI RAATTY 1 cut(s) 516
AsiGI ACCGGT 1 cut(s) 56
AspLEI GCGC 3 cut(s) 131, 506, 639
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 521
AxyI CCTNAGG 1 cut(s) 418
BaeGI GKGCMC 1 cut(s) 123
BanI GGYRCC 2 cut(s) 574, 636
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 3 cut(s) 283, 503, 706
BccI CCATC 5 cut(s) 18, 390, 608, 665, 749
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 696
BcoDI GTCTC 2 cut(s) 33, 256
BfaI CTAG 2 cut(s) 98, 781
BfmI CTRYAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 640
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 4 cut(s) 297, 492, 720, 775
BlsI GCNGC 4 cut(s) 298, 493, 721, 776
Bme1390I CCNGG 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 576, 638
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 3 cut(s) 17, 173, 331
BsaHI GRCGYC 1 cut(s) 637
BsaI GGTCTC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 578
BsaWI WCCGGW 2 cut(s) 56, 593
BsaXI ACNNNNNCTCC 4 cut(s) 102, 132, 588, 618
Bse118I RCCGGY 2 cut(s) 56, 455
Bse1I ACTGG 1 cut(s) 199
Bse21I CCTNAGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 4 cut(s) 279, 400, 510, 657
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 244
BseSI GKGCMC 1 cut(s) 123
BseXI GCAGC 3 cut(s) 283, 503, 706
BshFI GGCC 1 cut(s) 416
BshNI GGYRCC 2 cut(s) 574, 636
BshTI ACCGGT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 3 cut(s) 57, 456, 594
BsmAI GTCTC 2 cut(s) 33, 256
BsnI GGCC 1 cut(s) 416
Bso31I GGTCTC 1 cut(s) 33
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 1 cut(s) 696
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 1 cut(s) 774
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 576, 638
BspMI ACCTGC 1 cut(s) 134
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 2 cut(s) 574, 636
BspTNI GGTCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 2 cut(s) 56, 455
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 2 cut(s) 56, 455
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 1 cut(s) 696
BssNI GRCGYC 1 cut(s) 637
BssT1I CCWWGG 1 cut(s) 578
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 4 cut(s) 118, 250, 480, 769
BstACI GRCGYC 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 133, 535
BstDEI CTNAG 2 cut(s) 311, 418
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 4 cut(s) 279, 400, 510, 657
BstH2I RGCGCY 1 cut(s) 640
BstHHI GCGC 3 cut(s) 131, 506, 639
BstKTI GATC 1 cut(s) 699
BstMAI GTCTC 2 cut(s) 33, 256
BstMBI GATC 1 cut(s) 696
BstMWI GCNNNNNNNGC 3 cut(s) 452, 491, 581
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 123
BstV1I GCAGC 3 cut(s) 283, 503, 706
Bsu36I CCTNAGG 1 cut(s) 418
BsuRI GGCC 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 578
BtsCI GGATG 4 cut(s) 279, 400, 510, 657
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 2 cut(s) 133, 535
CfoI GCGC 3 cut(s) 131, 506, 639
Cfr10I RCCGGY 2 cut(s) 56, 455
Csp6I GTAC 1 cut(s) 561
CspAI ACCGGT 1 cut(s) 56
CviAII CATG 3 cut(s) 40, 534, 579
CviJI RGCY 8 cut(s) 18, 160, 198, 416, 467, 584, 676, 777
CviKI_1 RGCY 8 cut(s) 18, 160, 198, 416, 467, 584, 676, 777
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 2 cut(s) 311, 418
DinI GGCGCC 1 cut(s) 638
DpnI GATC 1 cut(s) 698
DpnII GATC 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 578
Eco147I AGGCCT 1 cut(s) 416
Eco31I GGTCTC 1 cut(s) 33
Eco81I CCTNAGG 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 578
EgeI GGCGCC 1 cut(s) 638
EheI GGCGCC 1 cut(s) 638
ErhI CCWWGG 1 cut(s) 578
FaeI CATG 3 cut(s) 43, 537, 582
FaiI YATR 8 cut(s) 41, 81, 330, 353, 379, 489, 535, 580
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FatI CATG 3 cut(s) 39, 533, 578
FbaI TGATCA 1 cut(s) 696
Fnu4HI GCNGC 4 cut(s) 297, 492, 720, 775
FokI GGATG 4 cut(s) 266, 387, 497, 644
Fsp4HI GCNGC 4 cut(s) 297, 492, 720, 775
FspBI CTAG 2 cut(s) 98, 781
GlaI GCGC 3 cut(s) 130, 505, 638
GluI GCNGC 4 cut(s) 297, 492, 720, 775
HaeII RGCGCY 1 cut(s) 640
HaeIII GGCC 1 cut(s) 416
HapII CCGG 3 cut(s) 57, 456, 594
HhaI GCGC 3 cut(s) 131, 506, 639
Hin1I GRCGYC 1 cut(s) 637
Hin1II CATG 3 cut(s) 43, 537, 582
Hin6I GCGC 3 cut(s) 129, 504, 637
HinP1I GCGC 3 cut(s) 129, 504, 637
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HinfI GANTC 4 cut(s) 148, 183, 371, 608
HpaII CCGG 3 cut(s) 57, 456, 594
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 121, 589
Hpy188I TCNGA 4 cut(s) 77, 613, 625, 696
Hpy188III TCNNGA 4 cut(s) 152, 164, 409, 420
Hpy8I GTNNAC 2 cut(s) 121, 589
HpyAV CCTTC 3 cut(s) 260, 406, 722
HpyCH4III ACNGT 4 cut(s) 118, 250, 480, 769
HpyCH4V TGCA 7 cut(s) 121, 299, 306, 398, 428, 491, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 491, 581
HpyF3I CTNAG 2 cut(s) 311, 418
Hsp92I GRCGYC 1 cut(s) 637
Hsp92II CATG 3 cut(s) 43, 537, 582
HspAI GCGC 3 cut(s) 129, 504, 637
KasI GGCGCC 1 cut(s) 636
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 1 cut(s) 696
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 3 cut(s) 283, 503, 706
LweI GCATC 1 cut(s) 81
MaeI CTAG 2 cut(s) 98, 781
MaeIII GTNAC 3 cut(s) 52, 190, 206
MalI GATC 1 cut(s) 698
MboI GATC 1 cut(s) 696
MboII GAAGA 2 cut(s) 350, 535
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 1 cut(s) 516
Mly113I GGCGCC 1 cut(s) 637
MmeI TCCRAC 1 cut(s) 685
MseI TTAA 3 cut(s) 212, 276, 600
MslI CAYNNNNRTG 2 cut(s) 538, 624
MspI CCGG 3 cut(s) 57, 456, 594
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 452, 491, 581
NarI GGCGCC 1 cut(s) 637
NcoI CCATGG 1 cut(s) 578
NdeII GATC 1 cut(s) 696
NlaIII CATG 3 cut(s) 43, 537, 582
NlaIV GGNNCC 2 cut(s) 576, 638
NmuCI GTSAC 3 cut(s) 52, 190, 206
NspI RCATGY 1 cut(s) 537
NspV TTCGAA 1 cut(s) 521
PaeI GCATGC 1 cut(s) 537
PaqCI CACCTGC 1 cut(s) 134
PceI AGGCCT 1 cut(s) 416
PfeI GAWTC 4 cut(s) 148, 183, 371, 608
PinAI ACCGGT 1 cut(s) 56
PkrI GCNGC 4 cut(s) 298, 493, 721, 776
PluTI GGCGCC 1 cut(s) 640
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 576, 638
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
RseI CAYNNNNRTG 2 cut(s) 538, 624
SaqAI TTAA 3 cut(s) 212, 276, 600
SatI GCNGC 4 cut(s) 297, 492, 720, 775
Sau3AI GATC 1 cut(s) 696
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 123
SetI ASST 6 cut(s) 10, 128, 218, 240, 469, 649
SfaNI GCATC 1 cut(s) 81
SfcI CTRYAG 1 cut(s) 321
SfoI GGCGCC 1 cut(s) 638
SfuI TTCGAA 1 cut(s) 521
SgrAI CRCCGGYG 1 cut(s) 56
SmiMI CAYNNNNRTG 2 cut(s) 538, 624
SmlI CTYRAG 3 cut(s) 32, 152, 346
SmoI CTYRAG 3 cut(s) 32, 152, 346
SphI GCATGC 1 cut(s) 537
Sse9I AATT 1 cut(s) 516
SseBI AGGCCT 1 cut(s) 416
SsiI CCGC 1 cut(s) 774
SspDI GGCGCC 1 cut(s) 636
SspMI CTAG 2 cut(s) 98, 781
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 4 cut(s) 118, 250, 480, 769
TaqI TCGA 3 cut(s) 12, 521, 649
TasI AATT 1 cut(s) 516
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 4 cut(s) 148, 183, 371, 608
Tru1I TTAA 3 cut(s) 212, 276, 600
Tru9I TTAA 3 cut(s) 212, 276, 600
TseFI GTSAC 3 cut(s) 52, 190, 206
TseI GCWGC 3 cut(s) 296, 491, 719
Tsp45I GTSAC 3 cut(s) 52, 190, 206
TspDTI ATGAA 2 cut(s) 371, 696
VneI GTGCAC 1 cut(s) 119
XapI RAATTY 1 cut(s) 516
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XspI CTAG 2 cut(s) 98, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.