pycom04g12240

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001690
Physical Location & Seq
Forward (+)
15229518 .. 15230993
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12240.2

Sequence Viewer

Length: 783 bp
ATGGCCGGTTTCAACAGCCAAAGATGGTCTCTAAAGGGTATGACCGCCCTCGTCACTGGTGGAACTAAAGGCATCGGATATGCCATAGTAGAAGAGCTAGCAGGACTTGGTGCAACCGTGCATACTTGTGCTCGTAACGAAGCACAGATTGTTGAGAGGCTTCAAGAATGGGAAGATAAGGGATTCAAAGTGACAGGCTCAGTTTGTGATCTCACTTCTAAAGCTCAAAGAGAAAACCTCATCAAAACCGTCTCCTGTATTTTCAATGGCAAGCTTAACATCCTTGTAAATAATGCTGCAATATGTACTCTCAGAAGTACTGCAGATTACACTTTGGAAGATTTTTCAGGTATGATGGGCACCAATGTTGAATCTCCCTACCATCTTTCTCAACTTGCATATCCTCTCTTGAAAGCCTCAGAAGATGCAAGTATTGTATTTGTCTCCTCTATTGCTGGTCTGAAAGCTCTACCCAGACTATCTGCCTATGCAGCAACCAAAGGAGCAGTGATACAGATTGCAAAAAATTTGGCATGTGAATGGGCAAAAGACAACATTCGTACAAACGCTGTGGCACCATGGGCTGTCAACACTGGGGCTAAAGTCGAAAATGATGGTCATGCTGAAGATTTCAGACGGCTGATAGGTCGAACTCCCATCCGTCGTCTTGCACAGCCTAATGAAATTTCGTCTCTGGTGTCCTTCCTTTGCCTCCCTGCTGCTTCTTTCATCAATGGACAAGTGATTAGCGTTGATGGAGGATTCACTGTTAGTGGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

261

Amino Acids

27.85

Weight (kDa)

8.19

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 359, 574
AciI CCGC 1 cut(s) 45
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 526, 684
AcuI CTGAAG 1 cut(s) 645
AfaI GTAC 3 cut(s) 307, 319, 562
AgsI TTSAA 6 cut(s) 13, 164, 187, 265, 371, 412
AluBI AGCT 4 cut(s) 97, 224, 274, 467
AluI AGCT 4 cut(s) 97, 224, 274, 467
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 4 cut(s) 33, 256, 448, 696
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 296, 491, 719
ApoI RAATTY 2 cut(s) 526, 684
AsuNHI GCTAGC 1 cut(s) 97
BaeGI GKGCMC 1 cut(s) 362
BanI GGYRCC 2 cut(s) 359, 574
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 3 cut(s) 283, 503, 706
BccI CCATC 6 cut(s) 18, 349, 390, 608, 665, 749
BceAI ACGGC 1 cut(s) 653
BcoDI GTCTC 4 cut(s) 33, 256, 448, 696
BfaI CTAG 2 cut(s) 98, 781
BfmI CTRYAG 1 cut(s) 321
BisI GCNGC 3 cut(s) 297, 492, 720
BlsI GCNGC 3 cut(s) 298, 493, 721
BmcAI AGTACT 1 cut(s) 319
BmiI GGNNCC 2 cut(s) 361, 576
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 2 cut(s) 81, 415
BmtI GCTAGC 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 603
BplI GAGNNNNNCTC 2 cut(s) 222, 254
BsaI GGTCTC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 578
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 2 cut(s) 61, 598
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 2 cut(s) 279, 657
BseMII CTCAG 3 cut(s) 213, 325, 432
BseNI ACTGG 2 cut(s) 61, 598
BseRI GAGGAG 1 cut(s) 436
BseSI GKGCMC 1 cut(s) 362
BseXI GCAGC 3 cut(s) 283, 503, 706
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 359, 574
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 1 cut(s) 6
BsmAI GTCTC 4 cut(s) 33, 256, 448, 696
BsmBI CGTCTC 2 cut(s) 256, 696
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 133, 362
Bsp143I GATC 1 cut(s) 208
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 212, 324, 431
BspLI GGNNCC 2 cut(s) 361, 576
BspMAI CTGCAG 1 cut(s) 325
BspOI GCTAGC 1 cut(s) 101
BspQI GCTCTTC 1 cut(s) 87
BspT107I GGYRCC 2 cut(s) 359, 574
BspTNI GGTCTC 1 cut(s) 33
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 2 cut(s) 61, 598
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 1 cut(s) 208
BssT1I CCWWGG 1 cut(s) 578
Bst4CI ACNGT 3 cut(s) 118, 250, 769
Bst6I CTCTTC 1 cut(s) 87
BstC8I GCNNGC 2 cut(s) 99, 272
BstDEI CTNAG 3 cut(s) 199, 311, 418
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 2 cut(s) 279, 657
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 4 cut(s) 33, 256, 448, 696
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 2 cut(s) 491, 581
BstNSI RCATGY 1 cut(s) 537
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 362
BstV1I GCAGC 3 cut(s) 283, 503, 706
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 578
BtsCI GGATG 2 cut(s) 279, 657
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 4 cut(s) 54, 513, 591, 765
Cac8I GCNNGC 2 cut(s) 99, 272
Cfr10I RCCGGY 1 cut(s) 5
Csp6I GTAC 3 cut(s) 306, 318, 561
CspCI CAANNNNNGTGG 2 cut(s) 552, 587
CviAII CATG 3 cut(s) 534, 579, 620
CviQI GTAC 3 cut(s) 306, 318, 561
DdeI CTNAG 3 cut(s) 199, 311, 418
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 578
Eco31I GGTCTC 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 578
Esp3I CGTCTC 2 cut(s) 256, 696
FaeI CATG 3 cut(s) 537, 582, 623
FatI CATG 3 cut(s) 533, 578, 619
Fnu4HI GCNGC 3 cut(s) 297, 492, 720
FokI GGATG 2 cut(s) 266, 644
Fsp4HI GCNGC 3 cut(s) 297, 492, 720
FspBI CTAG 2 cut(s) 98, 781
GluI GCNGC 3 cut(s) 297, 492, 720
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 6
Hin1II CATG 3 cut(s) 537, 582, 623
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 3 cut(s) 183, 371, 762
HpaII CCGG 1 cut(s) 6
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 5 cut(s) 77, 314, 421, 462, 635
Hpy188III TCNNGA 2 cut(s) 164, 409
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 666
HpyAV CCTTC 1 cut(s) 712
HpyCH4III ACNGT 3 cut(s) 118, 250, 769
HpyCH4V TGCA 9 cut(s) 113, 121, 299, 323, 398, 428, 491, 521, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 581
HpyF3I CTNAG 3 cut(s) 199, 311, 418
Hsp92II CATG 3 cut(s) 537, 582, 623
Kzo9I GATC 1 cut(s) 208
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 3 cut(s) 283, 503, 706
LweI GCATC 2 cut(s) 81, 415
MaeI CTAG 2 cut(s) 98, 781
MaeIII GTNAC 3 cut(s) 52, 134, 190
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 5 cut(s) 104, 185, 350, 434, 638
MhlI GDGCHC 2 cut(s) 133, 362
MluCI AATT 2 cut(s) 526, 684
MnlI CCTC 8 cut(s) 59, 150, 248, 414, 427, 457, 722, 752
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 2 cut(s) 126, 538
MspI CCGG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 491, 581
NcoI CCATGG 1 cut(s) 578
NdeII GATC 1 cut(s) 208
NheI GCTAGC 1 cut(s) 97
NlaIII CATG 3 cut(s) 537, 582, 623
NlaIV GGNNCC 2 cut(s) 361, 576
NmuCI GTSAC 2 cut(s) 52, 190
NspI RCATGY 1 cut(s) 537
PciSI GCTCTTC 1 cut(s) 87
PfeI GAWTC 3 cut(s) 183, 371, 762
PkrI GCNGC 3 cut(s) 298, 493, 721
PspN4I GGNNCC 2 cut(s) 361, 576
PstI CTGCAG 1 cut(s) 325
RsaI GTAC 3 cut(s) 307, 319, 562
RsaNI GTAC 3 cut(s) 306, 318, 561
RseI CAYNNNNRTG 2 cut(s) 126, 538
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 3 cut(s) 297, 492, 720
Sau3AI GATC 1 cut(s) 208
ScaI AGTACT 1 cut(s) 319
SduI GDGCHC 2 cut(s) 133, 362
SetI ASST 7 cut(s) 99, 226, 240, 276, 352, 469, 649
SfaNI GCATC 2 cut(s) 81, 415
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 2 cut(s) 126, 538
Sse9I AATT 2 cut(s) 526, 684
SsiI CCGC 1 cut(s) 45
SspMI CTAG 2 cut(s) 98, 781
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 3 cut(s) 118, 250, 769
TaqI TCGA 2 cut(s) 606, 649
TasI AATT 2 cut(s) 526, 684
TatI WGTACW 2 cut(s) 305, 317
TfiI GAWTC 3 cut(s) 183, 371, 762
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 4 cut(s) 61, 513, 598, 772
TseFI GTSAC 2 cut(s) 52, 190
TseI GCWGC 3 cut(s) 296, 491, 719
Tsp45I GTSAC 2 cut(s) 52, 190
TspDTI ATGAA 2 cut(s) 696, 718
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 4 cut(s) 61, 513, 598, 772
XapI RAATTY 2 cut(s) 526, 684
XceI RCATGY 1 cut(s) 537
XspI CTAG 2 cut(s) 98, 781
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.