pycom12g14270

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001690
Physical Location & Seq
Forward (+)
16844742 .. 16845980
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14270.5

Sequence Viewer

Length: 783 bp
ATGGCAGGTTTCGACAGCCAAAGATGGTCACTACAGGGTATGACTGCCCTCGTCACCGGTGGAACCAAAGGCATAGGATATGCCATAGTGGAAGAACTAGCCGGACTTGGAGCAACCGTGCATACCTGTGCTCGTAACAAAACACAGATTCTTGAGAGGCTTAAAGAATGGGAAAATAAGGGATTCAACGTGACCGGCTCAGTTTGTGATCTCACCTCTAAAGCACAAAGAGAGAACCTCATCAAAACTGTTTCCTCTATTTTTGATGGAAAACTTAACATCCTTGTAAATAGTGCCGCAACATGCACACTTAGAGGAACCACAGATTACACTTTGGAAGATTTCTCAACTATGATGGAAACCAATGTTGAATCTCCCTACCATCTTTCTCAACTTGCACACCCCCTCTTGAAGGCCTCAGGAAATGCAAGTATTGTCTTTGTTGCCTCCATTGCCGGTGTGGTAGCTCTACCGAAACTCTCTGCCTATGCAGCAACAAAAAGTGCAGTCATCCAAATTTCGAAGAACTTGGCATGTGAATGGGCAAAAGACAGCATTCGTACTAATGCTGTGGCGCCATGGGCTGTCAACACCGGAGTTAAAGTTGAATCTGACGGTCATTCTGATGATTTCAGACGCCTAATAGGTCGAACTCCCATCCGTCGCCTTGCACAGCCTAATGAAATCTCATCTCTGGTCACTTTCCTCTGCCTTCCTGCTGCGTCTTACATCAATGGACAAGTTATTAGCGTTGACGGAGGATTTACCGTTAGCGGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

261

Amino Acids

27.8

Weight (kDa)

8.25

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 574
AciI CCGC 2 cut(s) 297, 774
AcsI RAATTY 1 cut(s) 516
AcyI GRCGYC 2 cut(s) 575, 637
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 412
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 4 cut(s) 187, 371, 412, 608
AluBI AGCT 1 cut(s) 467
AluI AGCT 1 cut(s) 467
Alw21I GWGCWC 1 cut(s) 133
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 491, 719
ApoI RAATTY 1 cut(s) 516
AsiGI ACCGGT 1 cut(s) 56
AspLEI GCGC 1 cut(s) 577
AsuHPI GGTGA 2 cut(s) 46, 205
AsuII TTCGAA 1 cut(s) 521
AxyI CCTNAGG 1 cut(s) 418
BanI GGYRCC 1 cut(s) 574
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 503, 706
BccI CCATC 5 cut(s) 18, 260, 349, 390, 665
BfaI CTAG 2 cut(s) 98, 781
BfmI CTRYAG 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 578
BisI GCNGC 3 cut(s) 297, 492, 720
BlsI GCNGC 3 cut(s) 298, 493, 721
BmiI GGNNCC 3 cut(s) 64, 319, 576
BplI GAGNNNNNCTC 2 cut(s) 222, 254
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 1 cut(s) 173
BsaHI GRCGYC 2 cut(s) 575, 637
BsaJI CCNNGG 1 cut(s) 578
BsaWI WCCGGW 2 cut(s) 56, 593
BsaXI ACNNNNNCTCC 4 cut(s) 102, 132, 588, 618
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse118I RCCGGY 3 cut(s) 56, 194, 455
Bse21I CCTNAGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 450
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 3 cut(s) 279, 510, 657
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 2 cut(s) 213, 432
BseXI GCAGC 2 cut(s) 503, 706
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 1 cut(s) 416
BshNI GGYRCC 1 cut(s) 574
BshTI ACCGGT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 5 cut(s) 57, 102, 195, 456, 594
BslI CCNNNNNNNGG 1 cut(s) 412
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 1 cut(s) 416
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 1 cut(s) 208
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 2 cut(s) 297, 774
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 2 cut(s) 212, 431
BspLI GGNNCC 3 cut(s) 64, 319, 576
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 1 cut(s) 574
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 3 cut(s) 56, 194, 455
BssAI RCCGGY 3 cut(s) 56, 194, 455
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 1 cut(s) 208
BssNI GRCGYC 2 cut(s) 575, 637
BssT1I CCWWGG 1 cut(s) 578
Bst4CI ACNGT 4 cut(s) 118, 250, 617, 769
BstACI GRCGYC 2 cut(s) 575, 637
BstBI TTCGAA 1 cut(s) 521
BstDEI CTNAG 3 cut(s) 199, 311, 418
BstDSI CCRYGG 1 cut(s) 578
BstENI CCTNNNNNAGG 1 cut(s) 410
BstF5I GGATG 3 cut(s) 279, 510, 657
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 3 cut(s) 452, 491, 581
BstNSI RCATGY 2 cut(s) 306, 537
BstSFI CTRYAG 1 cut(s) 32
BstV1I GCAGC 2 cut(s) 503, 706
Bsu36I CCTNAGG 1 cut(s) 418
BsuRI GGCC 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 578
BtsCI GGATG 3 cut(s) 279, 510, 657
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 3 cut(s) 56, 194, 455
CseI GACGC 2 cut(s) 645, 711
Csp6I GTAC 1 cut(s) 561
CspAI ACCGGT 1 cut(s) 56
CviAII CATG 3 cut(s) 303, 534, 579
CviJI RGCY 8 cut(s) 18, 101, 160, 198, 416, 467, 584, 676
CviKI_1 RGCY 8 cut(s) 18, 101, 160, 198, 416, 467, 584, 676
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 3 cut(s) 199, 311, 418
DinI GGCGCC 1 cut(s) 576
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 578
Eco147I AGGCCT 1 cut(s) 416
Eco81I CCTNAGG 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 578
EgeI GGCGCC 1 cut(s) 576
EheI GGCGCC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 578
FaeI CATG 3 cut(s) 306, 537, 582
FatI CATG 3 cut(s) 302, 533, 578
Fnu4HI GCNGC 3 cut(s) 297, 492, 720
FokI GGATG 3 cut(s) 266, 497, 644
Fsp4HI GCNGC 3 cut(s) 297, 492, 720
FspBI CTAG 2 cut(s) 98, 781
GlaI GCGC 1 cut(s) 576
GluI GCNGC 3 cut(s) 297, 492, 720
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 1 cut(s) 416
HapII CCGG 5 cut(s) 57, 102, 195, 456, 594
HgaI GACGC 2 cut(s) 645, 711
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 2 cut(s) 575, 637
Hin1II CATG 3 cut(s) 306, 537, 582
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 2 cut(s) 589, 754
HindII GTYRAC 2 cut(s) 589, 754
HinfI GANTC 4 cut(s) 148, 183, 371, 608
HpaII CCGG 5 cut(s) 57, 102, 195, 456, 594
HphI GGTGA 2 cut(s) 46, 205
Hpy166II GTNNAC 2 cut(s) 589, 754
Hpy188I TCNGA 3 cut(s) 613, 625, 635
Hpy188III TCNNGA 3 cut(s) 152, 409, 420
Hpy8I GTNNAC 2 cut(s) 589, 754
Hpy99I CGWCG 1 cut(s) 666
HpyAV CCTTC 2 cut(s) 406, 722
HpyCH4III ACNGT 4 cut(s) 118, 250, 617, 769
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 7 cut(s) 121, 306, 398, 428, 491, 506, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 491, 581
HpyF3I CTNAG 3 cut(s) 199, 311, 418
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 2 cut(s) 575, 637
Hsp92II CATG 3 cut(s) 306, 537, 582
HspAI GCGC 1 cut(s) 575
KasI GGCGCC 1 cut(s) 574
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 2 cut(s) 503, 706
MaeI CTAG 2 cut(s) 98, 781
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 5 cut(s) 27, 52, 134, 190, 697
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 3 cut(s) 104, 350, 535
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 1 cut(s) 516
Mly113I GGCGCC 1 cut(s) 575
MseI TTAA 3 cut(s) 162, 276, 600
MslI CAYNNNNRTG 3 cut(s) 126, 538, 624
MspI CCGG 5 cut(s) 57, 102, 195, 456, 594
Mva1269I GAATGC 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 452, 491, 581
NarI GGCGCC 1 cut(s) 575
NcoI CCATGG 1 cut(s) 578
NdeII GATC 1 cut(s) 208
NlaIII CATG 3 cut(s) 306, 537, 582
NlaIV GGNNCC 3 cut(s) 64, 319, 576
NmuCI GTSAC 4 cut(s) 27, 52, 190, 697
NspI RCATGY 2 cut(s) 306, 537
NspV TTCGAA 1 cut(s) 521
PceI AGGCCT 1 cut(s) 416
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 4 cut(s) 148, 183, 371, 608
PinAI ACCGGT 1 cut(s) 56
PkrI GCNGC 3 cut(s) 298, 493, 721
PluTI GGCGCC 1 cut(s) 578
PspN4I GGNNCC 3 cut(s) 64, 319, 576
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
RseI CAYNNNNRTG 3 cut(s) 126, 538, 624
SaqAI TTAA 3 cut(s) 162, 276, 600
SatI GCNGC 3 cut(s) 297, 492, 720
Sau3AI GATC 1 cut(s) 208
SduI GDGCHC 1 cut(s) 133
SetI ASST 7 cut(s) 10, 128, 192, 218, 240, 469, 649
SfcI CTRYAG 1 cut(s) 32
SfoI GGCGCC 1 cut(s) 576
SfuI TTCGAA 1 cut(s) 521
SgrAI CRCCGGYG 1 cut(s) 56
SmiMI CAYNNNNRTG 3 cut(s) 126, 538, 624
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 1 cut(s) 516
SseBI AGGCCT 1 cut(s) 416
SsiI CCGC 2 cut(s) 297, 774
SspDI GGCGCC 1 cut(s) 574
SspMI CTAG 2 cut(s) 98, 781
StuI AGGCCT 1 cut(s) 416
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 4 cut(s) 118, 250, 617, 769
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 12, 521, 649
TasI AATT 1 cut(s) 516
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 4 cut(s) 148, 183, 371, 608
Tru1I TTAA 3 cut(s) 162, 276, 600
Tru9I TTAA 3 cut(s) 162, 276, 600
TseFI GTSAC 4 cut(s) 27, 52, 190, 697
TseI GCWGC 2 cut(s) 491, 719
Tsp45I GTSAC 4 cut(s) 27, 52, 190, 697
TspDTI ATGAA 1 cut(s) 696
TspGWI ACGGA 2 cut(s) 650, 771
XagI CCTNNNNNAGG 1 cut(s) 410
XapI RAATTY 1 cut(s) 516
XceI RCATGY 2 cut(s) 306, 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XspI CTAG 2 cut(s) 98, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.