Rroxscaffold_6G00415060

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001690
Physical Location & Seq
Reverse (-)
37326781 .. 37328031
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00415060.1

Sequence Viewer

Length: 744 bp
ATGACCGCCCTCGTCACTGGTGGAACTAAAGGCATTGGATATGCCATCGTGGAAGAGCTAGCCGGACTAGGGGCTACAGTGCATACCTGCGCTCGTAATCAAGAACTGATTGACGAGAGGGTTCGAGAATGGAAGAGCAAGGGTTTTAAAGTGAGTGGCTCAGTGTGTGACCTCACATCTAAATCTCAAAGAGAGGAGCTCATGAGAACTGTGTCCTCTAATTTTGACGGCGAACTTAACATCCTTGTAAATAATGCAGCAACAGTCACTCTAAGAAATACTACAGAATATGATCTGGAAGATTTGTCAAGTATGATGAGCACAAATGTTGAATCCCCCTACCATCTTTGTCAACTTGCATATCCTCTCCTCAAGGCCTCCGGAAATGGAAGTATTGTATTCGTCGCCTCCATCGCTGGTTTAAAAGCTCTACCTAGACTATCCGCCTATGCAGCAACGAAAAGTGCCGTCATCCAGATTTCAAAGAACTTGGCGTGTGAATGGGCTAAAGACGACATTCGAACAAACGCTGTAGCACCTTGGGCTGTTAACACCGGAGTTGAAGTTGAAAACGATGACCACTCTGACGACTTCAGACGGCTAATAAGTCGAACTCCTATCCGTCGCGTTGCGGAACCTAATGAAATCTCATCACTGGTCACTTTTCTCTGCCTTCCTGCTGCTTCTTACATCAATGGACAAGTTGTTAGTGTTGATGTAGGATTTACCGTCAGCGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

247

Amino Acids

26.7

Weight (kDa)

5.32

Isoelectric Point (pI)

29.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 2 - 192 4.8e-50 short chain dehydrogenase
KR PF08659 2 - 156 3e-12 KR domain
SDR PF23441 2 - 186 2.3e-06 SDR-like rossmann domain
adh_short_C2 PF13561 10 - 243 9e-54 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 95
AccII CGCG 1 cut(s) 627
AccIII TCCGGA 1 cut(s) 380
AciI CCGC 4 cut(s) 6, 444, 632, 735
AcuI CTGAAG 1 cut(s) 577
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 4 cut(s) 332, 483, 563, 569
AloI GAACNNNNNNTCC 2 cut(s) 225, 257
AluBI AGCT 3 cut(s) 58, 199, 428
AluI AGCT 3 cut(s) 58, 199, 428
Alw21I GWGCWC 2 cut(s) 201, 323
Aor13HI TCCGGA 1 cut(s) 380
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 3 cut(s) 257, 452, 680
AspLEI GCGC 1 cut(s) 92
AsuII TTCGAA 1 cut(s) 520
AsuNHI GCTAGC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 323
BbvI GCAGC 3 cut(s) 269, 464, 667
BccI CCATC 3 cut(s) 53, 351, 419
BceAI ACGGC 3 cut(s) 244, 452, 614
BfaI CTAG 3 cut(s) 59, 68, 435
BfmI CTRYAG 3 cut(s) 75, 282, 531
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 3 cut(s) 258, 453, 681
BlsI GCNGC 3 cut(s) 259, 454, 682
BmiI GGNNCC 1 cut(s) 636
BmtI GCTAGC 1 cut(s) 62
BplI GAGNNNNNCTC 2 cut(s) 183, 215
Bpu14I TTCGAA 1 cut(s) 520
BpuEI CTTGAG 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 539
BsaWI WCCGGW 2 cut(s) 380, 554
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 22, 660
BseAI TCCGGA 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 2 cut(s) 240, 471
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 22, 660
BseRI GAGGAG 2 cut(s) 209, 359
BseXI GCAGC 3 cut(s) 269, 464, 667
Bsh1236I CGCG 1 cut(s) 627
BshFI GGCC 1 cut(s) 377
BsiHKAI GWGCWC 2 cut(s) 201, 323
BsiSI CCGG 3 cut(s) 63, 381, 555
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 1 cut(s) 377
Bsp119I TTCGAA 1 cut(s) 520
Bsp1286I GDGCHC 2 cut(s) 201, 323
Bsp13I TCCGGA 1 cut(s) 380
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 4 cut(s) 6, 444, 632, 735
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 1 cut(s) 173
BspEI TCCGGA 1 cut(s) 380
BspFNI CGCG 1 cut(s) 627
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 636
BspMI ACCTGC 1 cut(s) 95
BspOI GCTAGC 1 cut(s) 62
BspQI GCTCTTC 2 cut(s) 48, 128
BspT104I TTCGAA 1 cut(s) 520
BsrI ACTGG 2 cut(s) 22, 660
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 1 cut(s) 292
BssT1I CCWWGG 1 cut(s) 539
Bst4CI ACNGT 4 cut(s) 79, 211, 265, 730
Bst6I CTCTTC 2 cut(s) 48, 128
BstBI TTCGAA 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 160, 272
BstF5I GGATG 2 cut(s) 240, 471
BstFNI CGCG 1 cut(s) 627
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 3 cut(s) 413, 452, 542
BstSFI CTRYAG 3 cut(s) 75, 282, 531
BstUI CGCG 1 cut(s) 627
BstV1I GCAGC 3 cut(s) 269, 464, 667
BsuRI GGCC 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 397
BtsCI GGATG 2 cut(s) 240, 471
BtsIMutI CAGTG 4 cut(s) 15, 84, 168, 653
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 1 cut(s) 60
CciI TCATGA 1 cut(s) 201
CfoI GCGC 1 cut(s) 92
CviAII CATG 1 cut(s) 202
DdeI CTNAG 2 cut(s) 160, 272
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
DraI TTTAAA 2 cut(s) 148, 423
Eam1104I CTCTTC 2 cut(s) 48, 128
EarI CTCTTC 2 cut(s) 48, 128
EciI GGCGGA 1 cut(s) 433
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 539
Eco147I AGGCCT 1 cut(s) 377
Eco24I GRGCYC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 577
EcoICRI GAGCTC 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 539
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 1 cut(s) 205
FaiI YATR 7 cut(s) 42, 84, 203, 291, 314, 361, 450
FatI CATG 1 cut(s) 201
Fnu4HI GCNGC 3 cut(s) 258, 453, 681
FokI GGATG 2 cut(s) 227, 458
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 3 cut(s) 258, 453, 681
FspBI CTAG 3 cut(s) 59, 68, 435
GlaI GCGC 1 cut(s) 91
GluI GCNGC 3 cut(s) 258, 453, 681
HaeIII GGCC 1 cut(s) 377
HapII CCGG 3 cut(s) 63, 381, 555
HhaI GCGC 1 cut(s) 92
Hin1II CATG 1 cut(s) 205
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 2 cut(s) 353, 550
HindII GTYRAC 2 cut(s) 353, 550
HinfI GANTC 1 cut(s) 332
HpaI GTTAAC 1 cut(s) 550
HpaII CCGG 3 cut(s) 63, 381, 555
Hpy166II GTNNAC 2 cut(s) 353, 550
Hpy188I TCNGA 2 cut(s) 586, 596
Hpy188III TCNNGA 6 cut(s) 101, 125, 202, 296, 381, 475
Hpy8I GTNNAC 2 cut(s) 353, 550
Hpy99I CGWCG 2 cut(s) 407, 627
HpyAV CCTTC 1 cut(s) 683
HpyCH4III ACNGT 4 cut(s) 79, 211, 265, 730
HpyCH4V TGCA 4 cut(s) 82, 257, 359, 452
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 452, 542
HpyF3I CTNAG 2 cut(s) 160, 272
Hsp92II CATG 1 cut(s) 205
HspAI GCGC 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 380
KspAI GTTAAC 1 cut(s) 550
Kzo9I GATC 1 cut(s) 292
LguI GCTCTTC 2 cut(s) 48, 128
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 3 cut(s) 269, 464, 667
MaeI CTAG 3 cut(s) 59, 68, 435
MaeIII GTNAC 4 cut(s) 13, 167, 265, 658
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 3 cut(s) 65, 145, 311
MhlI GDGCHC 2 cut(s) 201, 323
MluCI AATT 1 cut(s) 220
MnlI CCTC 9 cut(s) 20, 111, 182, 187, 226, 375, 380, 388, 418
MroI TCCGGA 1 cut(s) 380
MseI TTAA 4 cut(s) 147, 237, 422, 549
MspA1I CMGCKG 1 cut(s) 735
MspI CCGG 3 cut(s) 63, 381, 555
MvnI CGCG 1 cut(s) 627
MwoI GCNNNNNNNGC 3 cut(s) 413, 452, 542
NdeII GATC 1 cut(s) 292
NheI GCTAGC 1 cut(s) 58
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 1 cut(s) 636
NmuCI GTSAC 4 cut(s) 13, 167, 265, 658
NspV TTCGAA 1 cut(s) 520
PagI TCATGA 1 cut(s) 201
PceI AGGCCT 1 cut(s) 377
PciSI GCTCTTC 2 cut(s) 48, 128
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 3 cut(s) 259, 454, 682
Psp124BI GAGCTC 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 636
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 2 cut(s) 48, 128
SaqAI TTAA 4 cut(s) 147, 237, 422, 549
SatI GCNGC 3 cut(s) 258, 453, 681
Sau3AI GATC 1 cut(s) 292
SduI GDGCHC 2 cut(s) 201, 323
SetI ASST 8 cut(s) 60, 89, 174, 201, 430, 436, 541, 640
SfcI CTRYAG 3 cut(s) 75, 282, 531
SfuI TTCGAA 1 cut(s) 520
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 1 cut(s) 220
SseBI AGGCCT 1 cut(s) 377
SsiI CCGC 4 cut(s) 6, 444, 632, 735
SspMI CTAG 3 cut(s) 59, 68, 435
SstI GAGCTC 1 cut(s) 201
StuI AGGCCT 1 cut(s) 377
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 4 cut(s) 79, 211, 265, 730
TaqI TCGA 3 cut(s) 124, 520, 610
TasI AATT 1 cut(s) 220
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 4 cut(s) 147, 237, 422, 549
Tru9I TTAA 4 cut(s) 147, 237, 422, 549
TscAI CASTG 4 cut(s) 22, 84, 168, 660
TseFI GTSAC 4 cut(s) 13, 167, 265, 658
TseI GCWGC 3 cut(s) 257, 452, 680
Tsp45I GTSAC 4 cut(s) 13, 167, 265, 658
TspDTI ATGAA 1 cut(s) 657
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 4 cut(s) 22, 84, 168, 660
XspI CTAG 3 cut(s) 59, 68, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.