Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001690

Physical Location & Seq

Genomic Coordinates Forward (+)

22895566 .. 22896850

Gene Structure 1285 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1148800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 783 bp

ATGGCAGGTTTCGACAGCCAAAGATGGTCACTACAGGGTATGACTGCCCTCGTCACCGGTGGAACCAAAGGCATAGGATATGCCATAGTGGAAGAACTAGCCGGACTTGGAGCAACCGTGCATACCTGTGCTCGTAACAAAACACAGATTCTTGAGAGGCTTAAAGAATGGGAAAATAAGGGATTCAACGTGACTGGCTCAGTTTGTGATCTCACCTCTAAAGCACAAAGAGAGAACCTCATCAAAACTGTTTCCTCTATTTTTGATGGAAAACTTAACATCCTTGTAAATAGTGCCGCAACATGCACACTTAGAGGAACCACAGATTACACCTTGGAAGATTTCTCAACTATGATGGAAACCAATGTCGAATCTCCCTACCATCTTTCTCAACTTGCACACCCTCTCTTGAAGGCCTCAGGAAATGCAAGTATTGTCTTTGTTGCCTCTATTGCCGGTGTGGTAGCTCTACCGAAACTCTCTGCCTATGCAGCAACAAAAAGTGCAGTCATCCAAATTTCGAAGAATTTGGCATGTGAATGGGCAAAAGACAGCATTCGTACTAATGCTGTGGCGCCATGGGCTGTCAACACCGGAGTTAAAGTTGAATCTGACGGTCATTCTGATGATTTCAGACGCCTAATAGGTCGAACTCCCATCCGTCGCCTTGCACAGCCTAATGAAATCTCATCTCTGGTCACTTTCCTCTGCCTTCCTGCTGCTTCTTACATCAATGGACAAGTTATTAGTGTTGACGGAGGATTTACCGTTAGCGGTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00960 ko01100 ko01110 map00960 map01100 map01110

Pfam Domains

Protein Families

adh_short adh_short_C2

Protein Analysis

261

Amino Acids

27.8

Weight (kDa)

8.25

Isoelectric Point (pI)

32.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SDR	PF23441	12 - 215	2.5e-06	SDR-like rossmann domain
adh_short	PF00106	15 - 203	3.1e-46	short chain dehydrogenase
KR	PF08659	15 - 169	5.7e-14	KR domain
adh_short_C2	PF13561	23 - 256	5.3e-54	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001690)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g13100 FvH4_6g13100 FvH4_6g13110 FvH4_6g13120 FvH4_6g21191
malus_domestica	MD04G1134900.v1.1 MD04G1135200.v1.1 MD04G1135300.v1.1 MD12G1148400.v1.1 MD12G1148500.v1.1 MD12G1148600.v1.1 MD12G1148800.v1.1 MD12G1149000.v1.1
pyrus_communis	pycom04g12230 pycom04g12240 pycom12g14270 pycom12g14280
rosa_chinensis	RchiOBHm_Chr3g0465411 RchiOBHm_Chr3g0465421 RchiOBHm_Chr3g0465491 RchiOBHm_Chr3g0477681
rosa_laevigata	RLG00000023701
rosa_multiflora	Rmu_sc0006187.1_g000004 Rmu_sc0011781.1_g000007 Rmu_sc0011781.1_g000008 Rmu_sc0011781.1_g000009
rosa_roxburghii	Rroxscaffold_6G00403760 Rroxscaffold_6G00415030 Rroxscaffold_6G00415050 Rroxscaffold_6G00415060
rosa_rugosa	Rorug03G0076400 Rorug03G0076500 Rorug03G0076600 Rorug03G0076700.1 Rorug03G0076800
rosa_samantha	Rh3AG212800 Rh3BG141700 Rh3BG141800 Rh3BG142100 Rh3BG246400 Rh3CG142800 Rh3CG142900 Rh3CG143400 Rh3CG240000 Rh3DG142800 Rh3DG142900 Rh3DG143200
rosa_wichuraiana	Rw3G011590 Rw3G019260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	574
AciI	CCGC	2 cut(s)	297, 774
AcsI	RAATTY	2 cut(s)	516, 526
AcyI	GRCGYC	2 cut(s)	575, 637
AfaI	GTAC	1 cut(s)	562
AgeI	ACCGGT	1 cut(s)	56
AgsI	TTSAA	3 cut(s)	187, 412, 608
AluBI	AGCT	1 cut(s)	467
AluI	AGCT	1 cut(s)	467
Alw21I	GWGCWC	1 cut(s)	133
AoxI	GGCC	1 cut(s)	414
ApeKI	GCWGC	2 cut(s)	491, 719
ApoI	RAATTY	2 cut(s)	516, 526
AsiGI	ACCGGT	1 cut(s)	56
AspLEI	GCGC	1 cut(s)	577
AsuHPI	GGTGA	2 cut(s)	46, 205
AsuII	TTCGAA	1 cut(s)	521
AxyI	CCTNAGG	1 cut(s)	418
BanI	GGYRCC	1 cut(s)	574
Bbv12I	GWGCWC	1 cut(s)	133
BbvI	GCAGC	2 cut(s)	503, 706
BccI	CCATC	5 cut(s)	18, 260, 349, 390, 665
BfaI	CTAG	2 cut(s)	98, 781
BfmI	CTRYAG	1 cut(s)	32
BfoI	RGCGCY	1 cut(s)	578
BisI	GCNGC	3 cut(s)	297, 492, 720
BlsI	GCNGC	3 cut(s)	298, 493, 721
BmiI	GGNNCC	3 cut(s)	64, 319, 576
BplI	GAGNNNNNCTC	2 cut(s)	222, 254
Bpu14I	TTCGAA	1 cut(s)	521
BpuEI	CTTGAG	1 cut(s)	173
BsaHI	GRCGYC	2 cut(s)	575, 637
BsaJI	CCNNGG	2 cut(s)	333, 578
BsaWI	WCCGGW	2 cut(s)	56, 593
BsaXI	ACNNNNNCTCC	4 cut(s)	102, 132, 588, 618
Bse118I	RCCGGY	2 cut(s)	56, 455
Bse1I	ACTGG	1 cut(s)	199
Bse21I	CCTNAGG	1 cut(s)	418
BseDI	CCNNGG	2 cut(s)	333, 578
BseGI	GGATG	3 cut(s)	279, 510, 657
BseMII	CTCAG	2 cut(s)	213, 432
BseNI	ACTGG	1 cut(s)	199
BseXI	GCAGC	2 cut(s)	503, 706
BsgI	GTGCAG	1 cut(s)	525
BshFI	GGCC	1 cut(s)	416
BshNI	GGYRCC	1 cut(s)	574
BshTI	ACCGGT	1 cut(s)	56
BsiHKAI	GWGCWC	1 cut(s)	133
BsiSI	CCGG	4 cut(s)	57, 102, 456, 594
BsmI	GAATGC	1 cut(s)	555
BsnI	GGCC	1 cut(s)	416
Bsp119I	TTCGAA	1 cut(s)	521
Bsp1286I	GDGCHC	1 cut(s)	133
Bsp143I	GATC	1 cut(s)	208
Bsp19I	CCATGG	1 cut(s)	578
BspACI	CCGC	2 cut(s)	297, 774
BspANI	GGCC	1 cut(s)	416
BspCNI	CTCAG	2 cut(s)	212, 431
BspLI	GGNNCC	3 cut(s)	64, 319, 576
BspT104I	TTCGAA	1 cut(s)	521
BspT107I	GGYRCC	1 cut(s)	574
BsrFI	RCCGGY	2 cut(s)	56, 455
BsrI	ACTGG	1 cut(s)	199
BssAI	RCCGGY	2 cut(s)	56, 455
BssECI	CCNNGG	2 cut(s)	333, 578
BssMI	GATC	1 cut(s)	208
BssNI	GRCGYC	2 cut(s)	575, 637
BssT1I	CCWWGG	2 cut(s)	333, 578
Bst4CI	ACNGT	4 cut(s)	118, 250, 617, 769
BstACI	GRCGYC	2 cut(s)	575, 637
BstBI	TTCGAA	1 cut(s)	521
BstDEI	CTNAG	3 cut(s)	199, 311, 418
BstDSI	CCRYGG	1 cut(s)	578
BstF5I	GGATG	3 cut(s)	279, 510, 657
BstH2I	RGCGCY	1 cut(s)	578
BstHHI	GCGC	1 cut(s)	577
BstKTI	GATC	1 cut(s)	211
BstMBI	GATC	1 cut(s)	208
BstMWI	GCNNNNNNNGC	3 cut(s)	452, 491, 581
BstNSI	RCATGY	2 cut(s)	306, 537
BstSFI	CTRYAG	1 cut(s)	32
BstV1I	GCAGC	2 cut(s)	503, 706
Bsu36I	CCTNAGG	1 cut(s)	418
BsuRI	GGCC	1 cut(s)	416
BtgI	CCRYGG	1 cut(s)	578
BtsCI	GGATG	3 cut(s)	279, 510, 657
CfoI	GCGC	1 cut(s)	577
Cfr10I	RCCGGY	2 cut(s)	56, 455
CseI	GACGC	1 cut(s)	645
Csp6I	GTAC	1 cut(s)	561
CspAI	ACCGGT	1 cut(s)	56
CviAII	CATG	3 cut(s)	303, 534, 579
CviJI	RGCY	8 cut(s)	18, 101, 160, 198, 416, 467, 584, 676
CviKI_1	RGCY	8 cut(s)	18, 101, 160, 198, 416, 467, 584, 676
CviQI	GTAC	1 cut(s)	561
DdeI	CTNAG	3 cut(s)	199, 311, 418
DinI	GGCGCC	1 cut(s)	576
DpnI	GATC	1 cut(s)	210
DpnII	GATC	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	333, 578
Eco147I	AGGCCT	1 cut(s)	416
Eco81I	CCTNAGG	1 cut(s)	418
EcoT14I	CCWWGG	2 cut(s)	333, 578
EgeI	GGCGCC	1 cut(s)	576
EheI	GGCGCC	1 cut(s)	576
ErhI	CCWWGG	2 cut(s)	333, 578
FaeI	CATG	3 cut(s)	306, 537, 582
FatI	CATG	3 cut(s)	302, 533, 578
Fnu4HI	GCNGC	3 cut(s)	297, 492, 720
FokI	GGATG	3 cut(s)	266, 497, 644
Fsp4HI	GCNGC	3 cut(s)	297, 492, 720
FspBI	CTAG	2 cut(s)	98, 781
GlaI	GCGC	1 cut(s)	576
GluI	GCNGC	3 cut(s)	297, 492, 720
HaeII	RGCGCY	1 cut(s)	578
HaeIII	GGCC	1 cut(s)	416
HapII	CCGG	4 cut(s)	57, 102, 456, 594
HgaI	GACGC	1 cut(s)	645
HhaI	GCGC	1 cut(s)	577
Hin1I	GRCGYC	2 cut(s)	575, 637
Hin1II	CATG	3 cut(s)	306, 537, 582
Hin6I	GCGC	1 cut(s)	575
HinP1I	GCGC	1 cut(s)	575
HincII	GTYRAC	2 cut(s)	589, 754
HindII	GTYRAC	2 cut(s)	589, 754
HinfI	GANTC	4 cut(s)	148, 183, 371, 608
HpaII	CCGG	4 cut(s)	57, 102, 456, 594
HphI	GGTGA	2 cut(s)	46, 205
Hpy166II	GTNNAC	2 cut(s)	589, 754
Hpy188I	TCNGA	3 cut(s)	613, 625, 635
Hpy188III	TCNNGA	3 cut(s)	152, 409, 420
Hpy8I	GTNNAC	2 cut(s)	589, 754
Hpy99I	CGWCG	1 cut(s)	666
HpyAV	CCTTC	2 cut(s)	406, 722
HpyCH4III	ACNGT	4 cut(s)	118, 250, 617, 769
HpyCH4IV	ACGT	1 cut(s)	189
HpyCH4V	TGCA	7 cut(s)	121, 306, 398, 428, 491, 506, 671
HpyF10VI	GCNNNNNNNGC	3 cut(s)	452, 491, 581
HpyF3I	CTNAG	3 cut(s)	199, 311, 418
HpySE526I	ACGT	1 cut(s)	189
Hsp92I	GRCGYC	2 cut(s)	575, 637
Hsp92II	CATG	3 cut(s)	306, 537, 582
HspAI	GCGC	1 cut(s)	575
KasI	GGCGCC	1 cut(s)	574
Kzo9I	GATC	1 cut(s)	208
LmnI	GCTCC	1 cut(s)	110
Lsp1109I	GCAGC	2 cut(s)	503, 706
MaeI	CTAG	2 cut(s)	98, 781
MaeII	ACGT	1 cut(s)	189
MaeIII	GTNAC	5 cut(s)	27, 52, 134, 190, 697
MalI	GATC	1 cut(s)	210
MboI	GATC	1 cut(s)	208
MboII	GAAGA	3 cut(s)	104, 350, 535
MhlI	GDGCHC	1 cut(s)	133
MluCI	AATT	2 cut(s)	516, 526
Mly113I	GGCGCC	1 cut(s)	575
MseI	TTAA	3 cut(s)	162, 276, 600
MslI	CAYNNNNRTG	3 cut(s)	126, 538, 624
MspI	CCGG	4 cut(s)	57, 102, 456, 594
Mva1269I	GAATGC	1 cut(s)	555
MwoI	GCNNNNNNNGC	3 cut(s)	452, 491, 581
NarI	GGCGCC	1 cut(s)	575
NcoI	CCATGG	1 cut(s)	578
NdeII	GATC	1 cut(s)	208
NlaIII	CATG	3 cut(s)	306, 537, 582
NlaIV	GGNNCC	3 cut(s)	64, 319, 576
NmuCI	GTSAC	4 cut(s)	27, 52, 190, 697
NspI	RCATGY	2 cut(s)	306, 537
NspV	TTCGAA	1 cut(s)	521
PceI	AGGCCT	1 cut(s)	416
PctI	GAATGC	1 cut(s)	555
PfeI	GAWTC	4 cut(s)	148, 183, 371, 608
PinAI	ACCGGT	1 cut(s)	56
PkrI	GCNGC	3 cut(s)	298, 493, 721
PluTI	GGCGCC	1 cut(s)	578
PspN4I	GGNNCC	3 cut(s)	64, 319, 576
RsaI	GTAC	1 cut(s)	562
RsaNI	GTAC	1 cut(s)	561
RseI	CAYNNNNRTG	3 cut(s)	126, 538, 624
SaqAI	TTAA	3 cut(s)	162, 276, 600
SatI	GCNGC	3 cut(s)	297, 492, 720
Sau3AI	GATC	1 cut(s)	208
SduI	GDGCHC	1 cut(s)	133
SetI	ASST	8 cut(s)	10, 128, 192, 218, 240, 335, 469, 649
SfcI	CTRYAG	1 cut(s)	32
SfoI	GGCGCC	1 cut(s)	576
SfuI	TTCGAA	1 cut(s)	521
SgrAI	CRCCGGYG	1 cut(s)	56
SmiMI	CAYNNNNRTG	3 cut(s)	126, 538, 624
SmlI	CTYRAG	1 cut(s)	152
SmoI	CTYRAG	1 cut(s)	152
Sse9I	AATT	2 cut(s)	516, 526
SseBI	AGGCCT	1 cut(s)	416
SsiI	CCGC	2 cut(s)	297, 774
SspDI	GGCGCC	1 cut(s)	574
SspMI	CTAG	2 cut(s)	98, 781
StuI	AGGCCT	1 cut(s)	416
StyI	CCWWGG	2 cut(s)	333, 578
TaaI	ACNGT	4 cut(s)	118, 250, 617, 769
TaiI	ACGT	1 cut(s)	192
TaqI	TCGA	4 cut(s)	12, 369, 521, 649
TasI	AATT	2 cut(s)	516, 526
TauI	GCSGC	1 cut(s)	299
TfiI	GAWTC	4 cut(s)	148, 183, 371, 608
Tru1I	TTAA	3 cut(s)	162, 276, 600
Tru9I	TTAA	3 cut(s)	162, 276, 600
TseFI	GTSAC	4 cut(s)	27, 52, 190, 697
TseI	GCWGC	2 cut(s)	491, 719
Tsp45I	GTSAC	4 cut(s)	27, 52, 190, 697
TspDTI	ATGAA	1 cut(s)	696
TspGWI	ACGGA	2 cut(s)	650, 771
XapI	RAATTY	2 cut(s)	516, 526
XceI	RCATGY	2 cut(s)	306, 537
XspI	CTAG	2 cut(s)	98, 781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.