Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002879

Physical Location & Seq

Genomic Coordinates Forward (+)

22756386 .. 22758195

Gene Structure 1810 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1139400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 666 bp

ATGGCTGAAGTGAAGCTGCTGGGATCTTGGGGAAGCCCATTTAGTAGGAGAGTGGAAGCAGCTCTGAAGCTAAAAAATGTGGAGTATGAATTTGTTGATGAGGACCTGCAGAGCAAGAGTGCTTTGCTTCTGAAATCCAACCCAGTTCATCAGAAGATCCCTGTGCTCCTGCACAATGACAGACCAATAGCTGAGTCCCAGGTCATTCTTGAGTACATTGATGAGACTTGGAAGGAGGGTTTTCCCATTTTGCCTAAAGATCCTTATGAAAGAGCTCAGGCACGTTTCTGGGCTAGGTTCATTGATGAAAAGTGCTTGCCTGCAACATGGAAAGCTCTCTGGGGCAGTGAGGAACCTGAGAAGGCTGTGGAAGAAGCCTGTGAGCTTCTGAAAATATTGGAAAATGAGCTCAAAGACAAGAAGTTCTTCGCAGGAGAAACCGTTGGACTTGTGGACATTGTTGCCAACTTCATAGCCTTCTGGCTTAGAGCCATTCAAGAACTTGTTGGAGTGGAGCTGTTGAGCAAAGAGAAGCTTCCCAAACTCTACAACTGGAGCGATGAGTTTTGTAGTGTTTTTCAGGAAAATCTGCCTCCCAAAGATAGATTAGTTGCTCACTTCCGTGGTCGCCTACAAAGCACCACCACCACTACTACTTCCAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009408 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009739 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016740 GO:0016765 GO:0019748 GO:0033993 GO:0034641 GO:0042221 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0051186 GO:0071704 GO:0097305 GO:0098754 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_N GST_N_3

Protein Analysis

222

Amino Acids

25.41

Weight (kDa)

5.31

Isoelectric Point (pI)

37.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	3 - 75	4.2e-19	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	7 - 76	2.8e-15	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	12 - 76	1.2e-13	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	116 - 191	5.6e-14	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	128 - 187	4.3e-06	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0002879)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09270
fragaria_vesca	FvH4_6g12680 FvH4_6g12690
malus_domestica	MD04G1139400.v1.1 MD04G1139500.v1.1 MD04G1139600.v1.1 MD12G1155200.v1.1
prunus_persica	Prupe.6G264900_v2.0.a1 Prupe.6G265000_v2.0.a1
pyrus_communis	pycom12g14730
rosa_chinensis	RchiOBHm_Chr3g0464521 RchiOBHm_Chr3g0464531 RchiOBHm_Chr3g0464541
rosa_laevigata	RLG00000024684 RLG00000024685 RLG00000024688
rosa_multiflora	Rmu_sc0002031.1_g000017 Rmu_sc0002031.1_g000018 Rmu_sc0002031.1_g000021 Rmu_sc0032314.1_g000002
rosa_roxburghii	Rroxscaffold_4G00321260 Rroxscaffold_6G00415560 Rroxscaffold_6G00415580 Rroxscaffold_6G00415590
rosa_rugosa	Rorug03G0008900 Rorug03G0008900 Rorug03G0072900 Rorug03G0073000 Rorug03G0073000
rosa_samantha	Rh3BG135200 Rh3CG137800 Rh3CG137900 Rh3DG136500 Rh3DG136700 Rh3DG136800
rosa_wichuraiana	Rw3G011050 Rw3G011060 Rw3G011070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	114
AclWI	GGATC	3 cut(s)	31, 151, 254
AcsI	RAATTY	1 cut(s)	89
AcuI	CTGAAG	2 cut(s)	27, 86
AfaI	GTAC	1 cut(s)	215
AgsI	TTSAA	1 cut(s)	497
AjnI	CCWGG	1 cut(s)	198
AleI	CACNNNNGTG	1 cut(s)	621
Alw21I	GWGCWC	3 cut(s)	168, 277, 411
Alw26I	GTCTC	1 cut(s)	218
AlwI	GGATC	3 cut(s)	31, 151, 254
ApeKI	GCWGC	2 cut(s)	16, 59
ApoI	RAATTY	1 cut(s)	89
Asp700I	GAANNNNTTC	1 cut(s)	425
AspS9I	GGNCC	1 cut(s)	103
AvaII	GGWCC	1 cut(s)	103
BanII	GRGCYC	2 cut(s)	277, 411
Bbv12I	GWGCWC	3 cut(s)	168, 277, 411
BbvI	GCAGC	2 cut(s)	3, 71
BciT130I	CCWGG	1 cut(s)	200
BcoDI	GTCTC	1 cut(s)	218
BfaI	CTAG	1 cut(s)	294
BfmI	CTRYAG	1 cut(s)	107
BfuAI	ACCTGC	1 cut(s)	114
BisI	GCNGC	2 cut(s)	17, 60
BlsI	GCNGC	2 cut(s)	18, 61
Bme1390I	CCNGG	1 cut(s)	200
Bme18I	GGWCC	1 cut(s)	103
BmgT120I	GGNCC	1 cut(s)	103
BmiI	GGNNCC	1 cut(s)	354
BmrFI	CCNGG	1 cut(s)	200
BmrI	ACTGGG	1 cut(s)	137
BmuI	ACTGGG	1 cut(s)	137
BpmI	CTGGAG	1 cut(s)	574
Bpu10I	CCTNAGC	1 cut(s)	276
BpuEI	CTTGAG	1 cut(s)	230
BsaJI	CCNNGG	2 cut(s)	198, 622
BsaXI	ACNNNNNCTCC	2 cut(s)	426, 456
Bse1I	ACTGG	2 cut(s)	143, 557
BseBI	CCWGG	1 cut(s)	200
BseDI	CCNNGG	2 cut(s)	198, 622
BseMII	CTCAG	3 cut(s)	183, 290, 348
BseNI	ACTGG	2 cut(s)	143, 557
BseXI	GCAGC	2 cut(s)	3, 71
BseYI	CCCAGC	1 cut(s)	19
BsgI	GTGCAG	1 cut(s)	155
BsiHKAI	GWGCWC	3 cut(s)	168, 277, 411
BslFI	GGGAC	1 cut(s)	181
BsmAI	GTCTC	1 cut(s)	218
BsmFI	GGGAC	1 cut(s)	181
Bsp1286I	GDGCHC	3 cut(s)	168, 277, 411
Bsp143I	GATC	3 cut(s)	23, 156, 259
BspCNI	CTCAG	3 cut(s)	184, 289, 349
BspLI	GGNNCC	1 cut(s)	354
BspMAI	CTGCAG	1 cut(s)	111
BspMI	ACCTGC	1 cut(s)	114
BspPI	GGATC	3 cut(s)	31, 151, 254
BsrI	ACTGG	2 cut(s)	143, 557
BssECI	CCNNGG	2 cut(s)	198, 622
BssMI	GATC	3 cut(s)	23, 156, 259
Bst2UI	CCWGG	1 cut(s)	200
Bst4CI	ACNGT	1 cut(s)	442
BstC8I	GCNNGC	2 cut(s)	317, 321
BstDEI	CTNAG	4 cut(s)	192, 276, 357, 485
BstDSI	CCRYGG	1 cut(s)	622
BstKTI	GATC	3 cut(s)	26, 159, 262
BstMAI	GTCTC	1 cut(s)	218
BstMBI	GATC	3 cut(s)	23, 156, 259
BstMWI	GCNNNNNNNGC	1 cut(s)	636
BstNI	CCWGG	1 cut(s)	200
BstSCI	CCNGG	1 cut(s)	198
BstSFI	CTRYAG	1 cut(s)	107
BstV1I	GCAGC	2 cut(s)	3, 71
BstX2I	RGATCY	3 cut(s)	23, 156, 259
BstYI	RGATCY	3 cut(s)	23, 156, 259
BtgI	CCRYGG	1 cut(s)	622
BtgZI	GCGATG	1 cut(s)	573
BtsI	GCAGTG	1 cut(s)	352
BtsIMutI	CAGTG	1 cut(s)	352
BveI	ACCTGC	1 cut(s)	114
Cac8I	GCNNGC	2 cut(s)	317, 321
Cfr13I	GGNCC	1 cut(s)	103
Csp6I	GTAC	1 cut(s)	214
CviAII	CATG	1 cut(s)	327
CviQI	GTAC	1 cut(s)	214
DdeI	CTNAG	4 cut(s)	192, 276, 357, 485
DpnI	GATC	3 cut(s)	25, 158, 261
DpnII	GATC	3 cut(s)	23, 156, 259
Ecl136II	GAGCTC	2 cut(s)	275, 409
Eco24I	GRGCYC	2 cut(s)	277, 411
Eco47I	GGWCC	1 cut(s)	103
Eco53kI	GAGCTC	2 cut(s)	275, 409
Eco57I	CTGAAG	2 cut(s)	27, 86
EcoICRI	GAGCTC	2 cut(s)	275, 409
EcoO109I	RGGNCCY	1 cut(s)	103
EcoRII	CCWGG	1 cut(s)	198
EcoT38I	GRGCYC	2 cut(s)	277, 411
FaeI	CATG	1 cut(s)	330
FaiI	YATR	4 cut(s)	87, 267, 328, 473
FalI	AAGNNNNNCTT	4 cut(s)	410, 442, 519, 551
FaqI	GGGAC	1 cut(s)	181
FatI	CATG	1 cut(s)	326
Fnu4HI	GCNGC	2 cut(s)	17, 60
FriOI	GRGCYC	2 cut(s)	277, 411
Fsp4HI	GCNGC	2 cut(s)	17, 60
FspBI	CTAG	1 cut(s)	294
GluI	GCNGC	2 cut(s)	17, 60
GsaI	CCCAGC	1 cut(s)	23
GsuI	CTGGAG	1 cut(s)	574
Hin1II	CATG	1 cut(s)	330
HindIII	AAGCTT	1 cut(s)	533
HinfI	GANTC	1 cut(s)	194
Hpy166II	GTNNAC	1 cut(s)	454
Hpy188I	TCNGA	4 cut(s)	66, 132, 153, 390
Hpy188III	TCNNGA	3 cut(s)	209, 497, 581
Hpy8I	GTNNAC	1 cut(s)	454
HpyAV	CCTTC	3 cut(s)	226, 355, 487
HpyCH4III	ACNGT	1 cut(s)	442
HpyCH4IV	ACGT	1 cut(s)	283
HpyCH4V	TGCA	3 cut(s)	109, 172, 323
HpyF10VI	GCNNNNNNNGC	1 cut(s)	636
HpyF3I	CTNAG	4 cut(s)	192, 276, 357, 485
HpySE526I	ACGT	1 cut(s)	283
Hsp92II	CATG	1 cut(s)	330
Kzo9I	GATC	3 cut(s)	23, 156, 259
LmnI	GCTCC	3 cut(s)	171, 514, 555
Lsp1109I	GCAGC	2 cut(s)	3, 71
MaeI	CTAG	1 cut(s)	294
MaeII	ACGT	1 cut(s)	283
MalI	GATC	3 cut(s)	25, 158, 261
MboI	GATC	3 cut(s)	23, 156, 259
MboII	GAAGA	3 cut(s)	166, 383, 418
MflI	RGATCY	3 cut(s)	23, 156, 259
MhlI	GDGCHC	3 cut(s)	168, 277, 411
MluCI	AATT	1 cut(s)	89
MlyI	GAGTC	1 cut(s)	203
MmeI	TCCRAC	3 cut(s)	162, 424, 487
MnlI	CCTC	4 cut(s)	94, 229, 343, 603
MroXI	GAANNNNTTC	1 cut(s)	425
MslI	CAYNNNNRTG	1 cut(s)	621
MspR9I	CCNGG	1 cut(s)	200
MvaI	CCWGG	1 cut(s)	200
MwoI	GCNNNNNNNGC	1 cut(s)	636
NdeII	GATC	3 cut(s)	23, 156, 259
NlaIII	CATG	1 cut(s)	330
NlaIV	GGNNCC	1 cut(s)	354
OliI	CACNNNNGTG	1 cut(s)	621
PdmI	GAANNNNTTC	1 cut(s)	425
PkrI	GCNGC	2 cut(s)	18, 61
PleI	GAGTC	1 cut(s)	202
PpsI	GAGTC	1 cut(s)	202
PpuMI	RGGWCCY	1 cut(s)	103
Psp124BI	GAGCTC	2 cut(s)	277, 411
Psp5II	RGGWCCY	1 cut(s)	103
Psp6I	CCWGG	1 cut(s)	198
PspFI	CCCAGC	1 cut(s)	19
PspGI	CCWGG	1 cut(s)	198
PspN4I	GGNNCC	1 cut(s)	354
PspPI	GGNCC	1 cut(s)	103
PspPPI	RGGWCCY	1 cut(s)	103
PstI	CTGCAG	1 cut(s)	111
PsuI	RGATCY	3 cut(s)	23, 156, 259
RsaI	GTAC	1 cut(s)	215
RsaNI	GTAC	1 cut(s)	214
RseI	CAYNNNNRTG	1 cut(s)	621
SacI	GAGCTC	2 cut(s)	277, 411
SatI	GCNGC	2 cut(s)	17, 60
Sau3AI	GATC	3 cut(s)	23, 156, 259
Sau96I	GGNCC	1 cut(s)	103
SchI	GAGTC	1 cut(s)	203
ScrFI	CCNGG	1 cut(s)	200
SduI	GDGCHC	3 cut(s)	168, 277, 411
SfcI	CTRYAG	1 cut(s)	107
SinI	GGWCC	1 cut(s)	103
SmiMI	CAYNNNNRTG	1 cut(s)	621
SmlI	CTYRAG	1 cut(s)	209
SmoI	CTYRAG	1 cut(s)	209
Sse9I	AATT	1 cut(s)	89
SspI	AATATT	1 cut(s)	396
SspMI	CTAG	1 cut(s)	294
SstI	GAGCTC	2 cut(s)	277, 411
StyD4I	CCNGG	1 cut(s)	198
TaaI	ACNGT	1 cut(s)	442
TaiI	ACGT	1 cut(s)	286
TasI	AATT	1 cut(s)	89
TatI	WGTACW	1 cut(s)	213
TscAI	CASTG	1 cut(s)	352
TseI	GCWGC	2 cut(s)	16, 59
TspDTI	ATGAA	6 cut(s)	102, 137, 282, 289, 321, 460
TspGWI	ACGGA	1 cut(s)	611
TspRI	CASTG	1 cut(s)	352
VpaK11BI	GGWCC	1 cut(s)	103
XapI	RAATTY	1 cut(s)	89
XmnI	GAANNNNTTC	1 cut(s)	425
XspI	CTAG	1 cut(s)	294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.