Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002879

Physical Location & Seq

Genomic Coordinates Forward (+)

22779085 .. 22780589

Gene Structure 1505 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1139600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 666 bp

ATGGCTGAAGAAGTGAAGCTGTTGGGAGTTTGGGGAAGCCCATTTAGTAGGAGGGTGGAAATAGCTCTGAAGCTGAAAGGAGTGAAATATGAATACTTTGAGGAGGACCTACAGAGCAAGAGCGCTTTGCTTCTGAAATCCAACCCAGTTCATCAGAAGATCCCTGTGCTGCTGCACAATGACAGACCAATAGCTGAGTCCCAGGTCATTCTTGAGTACATTGATCAGACTTGGAAGGAGGGTTTTCCCATTTTGCCTAAAGATCCTTATGAAAGAGCCCAGGCACGTTTCTGGGCTAGGTTCATTGATGAAAAGTGCTTGCCTGCAGCATGGAAAGCTCTCTGGGGCAGTGAGGAACCTGAGAAGGCTGTGGAAGAAGCCTGTGAGCTTCTGAAAATACTGGAAAATGAGCTCAAAGATAAGAAATTCTTCGGGGGAGAAACGGTTGGACTTGTGGACATTGTTGCCAACTTCATAGCCTACTGGCTTGGAGCCATTCAAGAAGTTGTGGGAGTGGAGCTGTTGACCAAAGAGAAGCTTCCCGATCTGTACGATTGGAGTGATGAGTTTTGCAGTGCTTTTCACGAAAGTCTGCCTCCCAGAGATAAACTAGTTACTTTCTTCCGTCGTCGCTTTCAGAGCACCACCACCGCTACTTCCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009408 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009739 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016740 GO:0016765 GO:0019748 GO:0033993 GO:0034641 GO:0042221 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0051186 GO:0071704 GO:0097305 GO:0098754 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_N GST_N_3

Protein Analysis

222

Amino Acids

25.5

Weight (kDa)

5.21

Isoelectric Point (pI)

45.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	4 - 76	1.2e-18	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	8 - 77	4.1e-15	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	13 - 76	6.9e-14	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	118 - 192	5.1e-13	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0002879)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09270
fragaria_vesca	FvH4_6g12680 FvH4_6g12690
malus_domestica	MD04G1139400.v1.1 MD04G1139500.v1.1 MD04G1139600.v1.1 MD12G1155200.v1.1
prunus_persica	Prupe.6G264900_v2.0.a1 Prupe.6G265000_v2.0.a1
pyrus_communis	pycom12g14730
rosa_chinensis	RchiOBHm_Chr3g0464521 RchiOBHm_Chr3g0464531 RchiOBHm_Chr3g0464541
rosa_laevigata	RLG00000024684 RLG00000024685 RLG00000024688
rosa_multiflora	Rmu_sc0002031.1_g000017 Rmu_sc0002031.1_g000018 Rmu_sc0002031.1_g000021 Rmu_sc0032314.1_g000002
rosa_roxburghii	Rroxscaffold_4G00321260 Rroxscaffold_6G00415560 Rroxscaffold_6G00415580 Rroxscaffold_6G00415590
rosa_rugosa	Rorug03G0008900 Rorug03G0008900 Rorug03G0072900 Rorug03G0073000 Rorug03G0073000
rosa_samantha	Rh3BG135200 Rh3CG137800 Rh3CG137900 Rh3DG136500 Rh3DG136700 Rh3DG136800
rosa_wichuraiana	Rw3G011050 Rw3G011060 Rw3G011070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	651
AclWI	GGATC	2 cut(s)	154, 257
AcsI	RAATTY	1 cut(s)	425
AcuI	CTGAAG	2 cut(s)	27, 89
AfaI	GTAC	2 cut(s)	218, 551
AfeI	AGCGCT	1 cut(s)	124
AgsI	TTSAA	1 cut(s)	500
AhlI	ACTAGT	1 cut(s)	610
AjnI	CCWGG	2 cut(s)	201, 279
AluBI	AGCT	9 cut(s)	19, 65, 73, 194, 338, 388, 412, 520, 538
AluI	AGCT	9 cut(s)	19, 65, 73, 194, 338, 388, 412, 520, 538
Alw21I	GWGCWC	2 cut(s)	414, 644
AlwI	GGATC	2 cut(s)	154, 257
Aor51HI	AGCGCT	1 cut(s)	124
ApeKI	GCWGC	3 cut(s)	169, 172, 326
ApoI	RAATTY	1 cut(s)	425
Asp700I	GAANNNNTTC	1 cut(s)	428
AspLEI	GCGC	1 cut(s)	125
AspS9I	GGNCC	1 cut(s)	106
AvaII	GGWCC	1 cut(s)	106
BanII	GRGCYC	2 cut(s)	280, 414
Bbv12I	GWGCWC	2 cut(s)	414, 644
BbvI	GCAGC	3 cut(s)	156, 159, 338
BciT130I	CCWGG	2 cut(s)	203, 281
BclI	TGATCA	1 cut(s)	223
BcuI	ACTAGT	1 cut(s)	610
BfaI	CTAG	2 cut(s)	297, 611
BfmI	CTRYAG	2 cut(s)	110, 324
BfoI	RGCGCY	1 cut(s)	126
BisI	GCNGC	3 cut(s)	170, 173, 327
BlsI	GCNGC	3 cut(s)	171, 174, 328
Bme1390I	CCNGG	2 cut(s)	203, 281
Bme18I	GGWCC	1 cut(s)	106
BmgT120I	GGNCC	1 cut(s)	106
BmiI	GGNNCC	2 cut(s)	357, 493
BmrFI	CCNGG	2 cut(s)	203, 281
BmrI	ACTGGG	1 cut(s)	140
BmuI	ACTGGG	1 cut(s)	140
BpuEI	CTTGAG	1 cut(s)	233
BsaJI	CCNNGG	2 cut(s)	201, 279
BsaXI	ACNNNNNCTCC	2 cut(s)	429, 459
Bse1I	ACTGG	3 cut(s)	146, 405, 488
BseBI	CCWGG	2 cut(s)	203, 281
BseDI	CCNNGG	2 cut(s)	201, 279
BseMII	CTCAG	2 cut(s)	186, 351
BseNI	ACTGG	3 cut(s)	146, 405, 488
BseRI	GAGGAG	1 cut(s)	116
BseXI	GCAGC	3 cut(s)	156, 159, 338
BsgI	GTGCAG	1 cut(s)	158
BsiHKAI	GWGCWC	2 cut(s)	414, 644
BslFI	GGGAC	1 cut(s)	184
BsmFI	GGGAC	1 cut(s)	184
Bsp1286I	GDGCHC	3 cut(s)	280, 414, 644
Bsp143I	GATC	4 cut(s)	159, 223, 262, 544
BspACI	CCGC	1 cut(s)	651
BspCNI	CTCAG	2 cut(s)	187, 352
BspLI	GGNNCC	2 cut(s)	357, 493
BspMAI	CTGCAG	1 cut(s)	328
BspPI	GGATC	2 cut(s)	154, 257
BsrI	ACTGG	3 cut(s)	146, 405, 488
BssECI	CCNNGG	2 cut(s)	201, 279
BssMI	GATC	4 cut(s)	159, 223, 262, 544
Bst2UI	CCWGG	2 cut(s)	203, 281
Bst4CI	ACNGT	1 cut(s)	445
BstC8I	GCNNGC	2 cut(s)	320, 324
BstDEI	CTNAG	2 cut(s)	195, 360
BstH2I	RGCGCY	1 cut(s)	126
BstHHI	GCGC	1 cut(s)	125
BstKTI	GATC	4 cut(s)	162, 226, 265, 547
BstMBI	GATC	4 cut(s)	159, 223, 262, 544
BstMWI	GCNNNNNNNGC	2 cut(s)	335, 639
BstNI	CCWGG	2 cut(s)	203, 281
BstSCI	CCNGG	2 cut(s)	201, 279
BstSFI	CTRYAG	2 cut(s)	110, 324
BstV1I	GCAGC	3 cut(s)	156, 159, 338
BstX2I	RGATCY	2 cut(s)	159, 262
BstYI	RGATCY	2 cut(s)	159, 262
BtsI	GCAGTG	2 cut(s)	355, 580
BtsIMutI	CAGTG	2 cut(s)	355, 580
Cac8I	GCNNGC	2 cut(s)	320, 324
CfoI	GCGC	1 cut(s)	125
Cfr13I	GGNCC	1 cut(s)	106
Csp6I	GTAC	2 cut(s)	217, 550
CviAII	CATG	1 cut(s)	330
CviQI	GTAC	2 cut(s)	217, 550
DdeI	CTNAG	2 cut(s)	195, 360
DpnI	GATC	4 cut(s)	161, 225, 264, 546
DpnII	GATC	4 cut(s)	159, 223, 262, 544
Ecl136II	GAGCTC	1 cut(s)	412
Eco24I	GRGCYC	2 cut(s)	280, 414
Eco47I	GGWCC	1 cut(s)	106
Eco47III	AGCGCT	1 cut(s)	124
Eco53kI	GAGCTC	1 cut(s)	412
Eco57I	CTGAAG	2 cut(s)	27, 89
EcoICRI	GAGCTC	1 cut(s)	412
EcoO109I	RGGNCCY	1 cut(s)	106
EcoRII	CCWGG	2 cut(s)	201, 279
EcoT38I	GRGCYC	2 cut(s)	280, 414
FaeI	CATG	1 cut(s)	333
FaiI	YATR	4 cut(s)	90, 270, 331, 476
FalI	AAGNNNNNCTT	4 cut(s)	413, 445, 522, 554
FaqI	GGGAC	1 cut(s)	184
FatI	CATG	1 cut(s)	329
FbaI	TGATCA	1 cut(s)	223
Fnu4HI	GCNGC	3 cut(s)	170, 173, 327
FriOI	GRGCYC	2 cut(s)	280, 414
Fsp4HI	GCNGC	3 cut(s)	170, 173, 327
FspBI	CTAG	2 cut(s)	297, 611
GlaI	GCGC	1 cut(s)	124
GluI	GCNGC	3 cut(s)	170, 173, 327
HaeII	RGCGCY	1 cut(s)	126
HhaI	GCGC	1 cut(s)	125
Hin1II	CATG	1 cut(s)	333
Hin6I	GCGC	1 cut(s)	123
HinP1I	GCGC	1 cut(s)	123
HincII	GTYRAC	1 cut(s)	525
HindII	GTYRAC	1 cut(s)	525
HindIII	AAGCTT	1 cut(s)	536
HinfI	GANTC	1 cut(s)	197
Hpy166II	GTNNAC	2 cut(s)	457, 525
Hpy188I	TCNGA	6 cut(s)	69, 135, 156, 228, 393, 639
Hpy188III	TCNNGA	4 cut(s)	212, 500, 542, 584
Hpy8I	GTNNAC	2 cut(s)	457, 525
Hpy99I	CGWCG	2 cut(s)	630, 633
HpyAV	CCTTC	2 cut(s)	229, 358
HpyCH4III	ACNGT	1 cut(s)	445
HpyCH4IV	ACGT	1 cut(s)	286
HpyCH4V	TGCA	3 cut(s)	175, 326, 573
HpyF10VI	GCNNNNNNNGC	2 cut(s)	335, 639
HpyF3I	CTNAG	2 cut(s)	195, 360
HpySE526I	ACGT	1 cut(s)	286
Hsp92II	CATG	1 cut(s)	333
HspAI	GCGC	1 cut(s)	123
Ksp22I	TGATCA	1 cut(s)	223
Kzo9I	GATC	4 cut(s)	159, 223, 262, 544
LmnI	GCTCC	2 cut(s)	491, 517
Lsp1109I	GCAGC	3 cut(s)	156, 159, 338
MaeI	CTAG	2 cut(s)	297, 611
MaeII	ACGT	1 cut(s)	286
MaeIII	GTNAC	1 cut(s)	613
MalI	GATC	4 cut(s)	161, 225, 264, 546
MboI	GATC	4 cut(s)	159, 223, 262, 544
MboII	GAAGA	5 cut(s)	20, 169, 386, 421, 613
MflI	RGATCY	2 cut(s)	159, 262
MhlI	GDGCHC	3 cut(s)	280, 414, 644
MluCI	AATT	1 cut(s)	425
MlyI	GAGTC	1 cut(s)	206
MmeI	TCCRAC	2 cut(s)	165, 427
MnlI	CCTC	6 cut(s)	45, 94, 97, 232, 346, 606
MroXI	GAANNNNTTC	1 cut(s)	428
MspR9I	CCNGG	2 cut(s)	203, 281
MvaI	CCWGG	2 cut(s)	203, 281
MwoI	GCNNNNNNNGC	2 cut(s)	335, 639
NdeII	GATC	4 cut(s)	159, 223, 262, 544
NlaIII	CATG	1 cut(s)	333
NlaIV	GGNNCC	2 cut(s)	357, 493
PdmI	GAANNNNTTC	1 cut(s)	428
PkrI	GCNGC	3 cut(s)	171, 174, 328
PleI	GAGTC	1 cut(s)	205
PpsI	GAGTC	1 cut(s)	205
PpuMI	RGGWCCY	1 cut(s)	106
Psp124BI	GAGCTC	1 cut(s)	414
Psp5II	RGGWCCY	1 cut(s)	106
Psp6I	CCWGG	2 cut(s)	201, 279
PspGI	CCWGG	2 cut(s)	201, 279
PspN4I	GGNNCC	2 cut(s)	357, 493
PspPI	GGNCC	1 cut(s)	106
PspPPI	RGGWCCY	1 cut(s)	106
PstI	CTGCAG	1 cut(s)	328
PsuI	RGATCY	2 cut(s)	159, 262
RsaI	GTAC	2 cut(s)	218, 551
RsaNI	GTAC	2 cut(s)	217, 550
SacI	GAGCTC	1 cut(s)	414
SatI	GCNGC	3 cut(s)	170, 173, 327
Sau3AI	GATC	4 cut(s)	159, 223, 262, 544
Sau96I	GGNCC	1 cut(s)	106
SchI	GAGTC	1 cut(s)	206
ScrFI	CCNGG	2 cut(s)	203, 281
SduI	GDGCHC	3 cut(s)	280, 414, 644
SfcI	CTRYAG	2 cut(s)	110, 324
SinI	GGWCC	1 cut(s)	106
SmlI	CTYRAG	1 cut(s)	212
SmoI	CTYRAG	1 cut(s)	212
SpeI	ACTAGT	1 cut(s)	610
Sse9I	AATT	1 cut(s)	425
SsiI	CCGC	1 cut(s)	651
SspMI	CTAG	2 cut(s)	297, 611
SstI	GAGCTC	1 cut(s)	414
StyD4I	CCNGG	2 cut(s)	201, 279
TaaI	ACNGT	1 cut(s)	445
TaiI	ACGT	1 cut(s)	289
TasI	AATT	1 cut(s)	425
TatI	WGTACW	1 cut(s)	216
TscAI	CASTG	2 cut(s)	355, 580
TseI	GCWGC	3 cut(s)	169, 172, 326
TspDTI	ATGAA	6 cut(s)	105, 140, 285, 292, 324, 463
TspGWI	ACGGA	1 cut(s)	614
TspRI	CASTG	2 cut(s)	355, 580
VpaK11BI	GGWCC	1 cut(s)	106
XapI	RAATTY	1 cut(s)	425
XmnI	GAANNNNTTC	1 cut(s)	428
XspI	CTAG	2 cut(s)	297, 611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.