Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002879

Physical Location & Seq

Genomic Coordinates Forward (+)

22765396 .. 22774986

Gene Structure 9591 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1139500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGGCTGAAGAGGTGAAGCTGTTGGGGGTTTGGGGAAGCCCATATAGTAGGAGAGTGGAAATAGCTCTGAAGCTGAAAAATGTGGAGTATGAATTTGTTGAGGAGGACCTGCAGAGCAAGAGTGCTTTGCTTCTGAAATCCAACCCAGTTCATCAGAAGATCCCTGTGCTCCTGCACAATGGCAAACCATTAGCTGAGTCCCAGGTCATTCTCGAGTACATTGATGAGACCTGGAAGGAGGGTTTCCCCATTTTGCCTAAAGATCCTTATGAAAGAGCCCAGGCACGTTTCTGGGCTAGGTTCATTGATGAAAAGTGCTTGCCTGCAACATATAAAGTTCTGTGGGGCTGTGAGGAACATGAAAAGGCTGTGGAAGAAGCTTGTGAGCTTCTGAAAATACTGGAAAATGAGCTCAAAGACAAGAAGTTCTTCGCAGGAGAGACGGTTGGACTTGTGGACATTGTTGCCAACTTCATAGGCTACTGGCTTAGAGCCATTCAAGAAGTTGTGGGAGTGGAGCTGTTGACCAAAGAGAAGCTTCCCAAACTGTACAATTGGAGCGATGAGTTTTGCAGTGTTTTTCAGGAAAGTCTGCCTCCCAAAGATAAACTAGTTGCACACATCCGTGGTCGCCTTCAAAGCACCAACACCACTACTACTTCAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009408 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009739 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016740 GO:0016765 GO:0019748 GO:0033993 GO:0034641 GO:0042221 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0051186 GO:0071704 GO:0097305 GO:0098754 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_N GST_N_3

Protein Analysis

223

Amino Acids

25.52

Weight (kDa)

5.43

Isoelectric Point (pI)

37.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	4 - 76	2.1e-20	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	8 - 77	7.2e-17	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	13 - 77	5.6e-15	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	119 - 192	6e-13	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0002879)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09270
fragaria_vesca	FvH4_6g12680 FvH4_6g12690
malus_domestica	MD04G1139400.v1.1 MD04G1139500.v1.1 MD04G1139600.v1.1 MD12G1155200.v1.1
prunus_persica	Prupe.6G264900_v2.0.a1 Prupe.6G265000_v2.0.a1
pyrus_communis	pycom12g14730
rosa_chinensis	RchiOBHm_Chr3g0464521 RchiOBHm_Chr3g0464531 RchiOBHm_Chr3g0464541
rosa_laevigata	RLG00000024684 RLG00000024685 RLG00000024688
rosa_multiflora	Rmu_sc0002031.1_g000017 Rmu_sc0002031.1_g000018 Rmu_sc0002031.1_g000021 Rmu_sc0032314.1_g000002
rosa_roxburghii	Rroxscaffold_4G00321260 Rroxscaffold_6G00415560 Rroxscaffold_6G00415580 Rroxscaffold_6G00415590
rosa_rugosa	Rorug03G0008900 Rorug03G0008900 Rorug03G0072900 Rorug03G0073000 Rorug03G0073000
rosa_samantha	Rh3BG135200 Rh3CG137800 Rh3CG137900 Rh3DG136500 Rh3DG136700 Rh3DG136800
rosa_wichuraiana	Rw3G011050 Rw3G011060 Rw3G011070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	117
AclWI	GGATC	2 cut(s)	154, 257
AcsI	RAATTY	1 cut(s)	92
AcuI	CTGAAG	2 cut(s)	27, 89
AfaI	GTAC	2 cut(s)	218, 551
AgsI	TTSAA	3 cut(s)	500, 638, 663
AhlI	ACTAGT	1 cut(s)	610
AjnI	CCWGG	3 cut(s)	201, 230, 279
AleI	CACNNNNGTG	1 cut(s)	624
Alw21I	GWGCWC	2 cut(s)	171, 414
Alw26I	GTCTC	2 cut(s)	221, 434
AlwI	GGATC	2 cut(s)	154, 257
Ama87I	CYCGRG	1 cut(s)	212
ApoI	RAATTY	1 cut(s)	92
Asp700I	GAANNNNTTC	1 cut(s)	428
AspS9I	GGNCC	1 cut(s)	106
AsuHPI	GGTGA	1 cut(s)	25
AvaI	CYCGRG	1 cut(s)	212
AvaII	GGWCC	1 cut(s)	106
BanII	GRGCYC	2 cut(s)	280, 414
Bbv12I	GWGCWC	2 cut(s)	171, 414
BciT130I	CCWGG	3 cut(s)	203, 232, 281
BcoDI	GTCTC	2 cut(s)	221, 434
BcuI	ACTAGT	1 cut(s)	610
BfaI	CTAG	2 cut(s)	297, 611
BfmI	CTRYAG	1 cut(s)	110
BfuAI	ACCTGC	1 cut(s)	117
Bme1390I	CCNGG	3 cut(s)	203, 232, 281
Bme18I	GGWCC	1 cut(s)	106
BmeT110I	CYCGRG	1 cut(s)	212
BmgT120I	GGNCC	1 cut(s)	106
BmrFI	CCNGG	3 cut(s)	203, 232, 281
BmrI	ACTGGG	1 cut(s)	140
BmuI	ACTGGG	1 cut(s)	140
BsaI	GGTCTC	1 cut(s)	221
BsaJI	CCNNGG	3 cut(s)	201, 279, 625
BsaXI	ACNNNNNCTCC	2 cut(s)	429, 459
Bse1I	ACTGG	3 cut(s)	146, 405, 488
BseBI	CCWGG	3 cut(s)	203, 232, 281
BseDI	CCNNGG	3 cut(s)	201, 279, 625
BseGI	GGATG	1 cut(s)	621
BseMII	CTCAG	1 cut(s)	186
BseNI	ACTGG	3 cut(s)	146, 405, 488
BseRI	GAGGAG	1 cut(s)	116
BsgI	GTGCAG	1 cut(s)	158
BsiHKAI	GWGCWC	2 cut(s)	171, 414
BsiHKCI	CYCGRG	1 cut(s)	212
BslFI	GGGAC	1 cut(s)	184
BsmAI	GTCTC	2 cut(s)	221, 434
BsmBI	CGTCTC	1 cut(s)	434
BsmFI	GGGAC	1 cut(s)	184
Bso31I	GGTCTC	1 cut(s)	221
BsoBI	CYCGRG	1 cut(s)	212
Bsp1286I	GDGCHC	3 cut(s)	171, 280, 414
Bsp1407I	TGTACA	1 cut(s)	549
Bsp143I	GATC	2 cut(s)	159, 262
BspCNI	CTCAG	1 cut(s)	187
BspMAI	CTGCAG	1 cut(s)	114
BspMI	ACCTGC	1 cut(s)	117
BspPI	GGATC	2 cut(s)	154, 257
BspTNI	GGTCTC	1 cut(s)	221
BsrGI	TGTACA	1 cut(s)	549
BsrI	ACTGG	3 cut(s)	146, 405, 488
BssECI	CCNNGG	3 cut(s)	201, 279, 625
BssMI	GATC	2 cut(s)	159, 262
Bst2UI	CCWGG	3 cut(s)	203, 232, 281
Bst4CI	ACNGT	2 cut(s)	445, 549
Bst6I	CTCTTC	1 cut(s)	3
BstAUI	TGTACA	1 cut(s)	549
BstC8I	GCNNGC	2 cut(s)	320, 324
BstDEI	CTNAG	2 cut(s)	195, 488
BstDSI	CCRYGG	1 cut(s)	625
BstF5I	GGATG	1 cut(s)	621
BstKTI	GATC	2 cut(s)	162, 265
BstMAI	GTCTC	2 cut(s)	221, 434
BstMBI	GATC	2 cut(s)	159, 262
BstMWI	GCNNNNNNNGC	1 cut(s)	639
BstNI	CCWGG	3 cut(s)	203, 232, 281
BstSCI	CCNGG	3 cut(s)	201, 230, 279
BstSFI	CTRYAG	1 cut(s)	110
BstX2I	RGATCY	2 cut(s)	159, 262
BstYI	RGATCY	2 cut(s)	159, 262
BtgI	CCRYGG	1 cut(s)	625
BtgZI	GCGATG	1 cut(s)	576
BtsCI	GGATG	1 cut(s)	621
BtsI	GCAGTG	1 cut(s)	580
BtsIMutI	CAGTG	1 cut(s)	580
BveI	ACCTGC	1 cut(s)	117
Cac8I	GCNNGC	2 cut(s)	320, 324
Cfr13I	GGNCC	1 cut(s)	106
Csp6I	GTAC	2 cut(s)	217, 550
CviAII	CATG	1 cut(s)	359
CviQI	GTAC	2 cut(s)	217, 550
DdeI	CTNAG	2 cut(s)	195, 488
DpnI	GATC	2 cut(s)	161, 264
DpnII	GATC	2 cut(s)	159, 262
Eam1104I	CTCTTC	1 cut(s)	3
EarI	CTCTTC	1 cut(s)	3
Ecl136II	GAGCTC	1 cut(s)	412
Eco24I	GRGCYC	2 cut(s)	280, 414
Eco31I	GGTCTC	1 cut(s)	221
Eco47I	GGWCC	1 cut(s)	106
Eco53kI	GAGCTC	1 cut(s)	412
Eco57I	CTGAAG	2 cut(s)	27, 89
Eco88I	CYCGRG	1 cut(s)	212
EcoICRI	GAGCTC	1 cut(s)	412
EcoO109I	RGGNCCY	1 cut(s)	106
EcoRII	CCWGG	3 cut(s)	201, 230, 279
EcoT38I	GRGCYC	2 cut(s)	280, 414
Esp3I	CGTCTC	1 cut(s)	434
FaeI	CATG	1 cut(s)	362
FaiI	YATR	8 cut(s)	43, 45, 90, 270, 331, 333, 360, 476
FalI	AAGNNNNNCTT	4 cut(s)	413, 445, 522, 554
FaqI	GGGAC	1 cut(s)	184
FatI	CATG	1 cut(s)	358
FokI	GGATG	1 cut(s)	608
FriOI	GRGCYC	2 cut(s)	280, 414
FspBI	CTAG	2 cut(s)	297, 611
Hin1II	CATG	1 cut(s)	362
HincII	GTYRAC	1 cut(s)	525
HindII	GTYRAC	1 cut(s)	525
HindIII	AAGCTT	2 cut(s)	378, 536
HinfI	GANTC	1 cut(s)	197
HphI	GGTGA	1 cut(s)	25
Hpy166II	GTNNAC	2 cut(s)	457, 525
Hpy188I	TCNGA	4 cut(s)	69, 135, 156, 393
Hpy188III	TCNNGA	3 cut(s)	212, 500, 584
Hpy8I	GTNNAC	2 cut(s)	457, 525
HpyAV	CCTTC	2 cut(s)	229, 644
HpyCH4III	ACNGT	2 cut(s)	445, 549
HpyCH4IV	ACGT	1 cut(s)	286
HpyCH4V	TGCA	5 cut(s)	112, 175, 326, 573, 617
HpyF10VI	GCNNNNNNNGC	1 cut(s)	639
HpyF3I	CTNAG	2 cut(s)	195, 488
HpySE526I	ACGT	1 cut(s)	286
Hsp92II	CATG	1 cut(s)	362
Kzo9I	GATC	2 cut(s)	159, 262
LmnI	GCTCC	3 cut(s)	174, 517, 558
MaeI	CTAG	2 cut(s)	297, 611
MaeII	ACGT	1 cut(s)	286
MalI	GATC	2 cut(s)	161, 264
MboI	GATC	2 cut(s)	159, 262
MboII	GAAGA	4 cut(s)	20, 169, 386, 421
MfeI	CAATTG	1 cut(s)	553
MflI	RGATCY	2 cut(s)	159, 262
MhlI	GDGCHC	3 cut(s)	171, 280, 414
MluCI	AATT	2 cut(s)	92, 553
MlyI	GAGTC	1 cut(s)	206
MmeI	TCCRAC	2 cut(s)	165, 427
MnlI	CCTC	6 cut(s)	4, 94, 97, 232, 346, 606
MroXI	GAANNNNTTC	1 cut(s)	428
MslI	CAYNNNNRTG	1 cut(s)	624
MspR9I	CCNGG	3 cut(s)	203, 232, 281
MunI	CAATTG	1 cut(s)	553
MvaI	CCWGG	3 cut(s)	203, 232, 281
MwoI	GCNNNNNNNGC	1 cut(s)	639
NdeII	GATC	2 cut(s)	159, 262
NlaIII	CATG	1 cut(s)	362
OliI	CACNNNNGTG	1 cut(s)	624
PaeR7I	CTCGAG	1 cut(s)	212
PdmI	GAANNNNTTC	1 cut(s)	428
PleI	GAGTC	1 cut(s)	205
PpsI	GAGTC	1 cut(s)	205
PpuMI	RGGWCCY	1 cut(s)	106
Psp124BI	GAGCTC	1 cut(s)	414
Psp5II	RGGWCCY	1 cut(s)	106
Psp6I	CCWGG	3 cut(s)	201, 230, 279
PspGI	CCWGG	3 cut(s)	201, 230, 279
PspPI	GGNCC	1 cut(s)	106
PspPPI	RGGWCCY	1 cut(s)	106
PstI	CTGCAG	1 cut(s)	114
PsuI	RGATCY	2 cut(s)	159, 262
RsaI	GTAC	2 cut(s)	218, 551
RsaNI	GTAC	2 cut(s)	217, 550
RseI	CAYNNNNRTG	1 cut(s)	624
SacI	GAGCTC	1 cut(s)	414
Sau3AI	GATC	2 cut(s)	159, 262
Sau96I	GGNCC	1 cut(s)	106
SchI	GAGTC	1 cut(s)	206
ScrFI	CCNGG	3 cut(s)	203, 232, 281
SduI	GDGCHC	3 cut(s)	171, 280, 414
SfcI	CTRYAG	1 cut(s)	110
Sfr274I	CTCGAG	1 cut(s)	212
SinI	GGWCC	1 cut(s)	106
SlaI	CTCGAG	1 cut(s)	212
SmiMI	CAYNNNNRTG	1 cut(s)	624
SmlI	CTYRAG	1 cut(s)	212
SmoI	CTYRAG	1 cut(s)	212
SpeI	ACTAGT	1 cut(s)	610
Sse9I	AATT	2 cut(s)	92, 553
SspMI	CTAG	2 cut(s)	297, 611
SstI	GAGCTC	1 cut(s)	414
StyD4I	CCNGG	3 cut(s)	201, 230, 279
TaaI	ACNGT	2 cut(s)	445, 549
TaiI	ACGT	1 cut(s)	289
TaqI	TCGA	1 cut(s)	213
TasI	AATT	2 cut(s)	92, 553
TatI	WGTACW	2 cut(s)	216, 549
TscAI	CASTG	1 cut(s)	580
TspDTI	ATGAA	7 cut(s)	105, 140, 285, 292, 324, 375, 463
TspGWI	ACGGA	1 cut(s)	614
TspRI	CASTG	1 cut(s)	580
VpaK11BI	GGWCC	1 cut(s)	106
XapI	RAATTY	1 cut(s)	92
XhoI	CTCGAG	1 cut(s)	212
XmnI	GAANNNNTTC	1 cut(s)	428
XspI	CTAG	2 cut(s)	297, 611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.