MD05G1020700.v1.1

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001021
Physical Location & Seq
Forward (+)
3521807 .. 3526513
4707 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1020700.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGGGGACGGCCTATGGAACCGGCAGCAGCAGCAGCAGCATCAACTTGGTCCCTCTGGCGGCGTGCATAAACGACCTCGTTCTGACTACGTTTCTTTGTGGTTTTCTTTTTGGTGTCAAGAGATGCATGGTTATTTTGCACGAGAGGATGATATTGGCGGTCCTAGGACTGTAAAGGATAGCAGATCAATCGGATCAGCATATGACCGCTATCTTCAGAATGCGGAAATGCCTTCGTACACTTCTGGAGAAGCGAGTACTTTTGGTGGAGCTGGAATGGGAAGGGCTGTTGGTGGTGGAATGTCAGGTCCTCCAATGGCTGATCATTCTATGAGGGGTCGTCCTGGAGCTATGTCTTCTGATCTACTAACAAATGGTCGAAATATGAATCTTGCTAATCAGCTCCCAGTAGATGCAACGGCTAGGCCTGGACGTGAAACAGTTCCTTTGCCTCTGGATGCATCCAGCACTTTATATGTTGAAGGACTTCCTCCTGACAGCACAAGGAGGGAAGTAGCTCATATCTTTCGTCCTTTTGTGGGATACAAAGCAGTGAGGCTTGTGAGCAAAGAGTCCAAACATCGTGGTGGGGACCCTCTTATTCTTTGTTTTGTGGATTTTGAAAATCCAGCCTGTGCAGCAACTGCTATGAGTGCGTTGCAAGGTTATGAAATGGACGAACACGCTCCAGATTCTAATTACTTGCGGCTGCAGTTTTCTCGGTTCCCAGGTCCCAGGTCTGGACCTGGACGTAACAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003712 GO:0003713 GO:0003723 GO:0003727 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006979 GO:0008143 GO:0008150 GO:0008152 GO:0008380 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010494 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010862 GO:0016070 GO:0016071 GO:0017069 GO:0019219 GO:0019220 GO:0019222 GO:0023051 GO:0023056 GO:0030532 GO:0030619 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0034641 GO:0035613 GO:0035614 GO:0035770 GO:0036464 GO:0042325 GO:0042327 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0045937 GO:0046483 GO:0046983 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0060390 GO:0060391 GO:0060393 GO:0065007 GO:0070013 GO:0070717 GO:0071704 GO:0080090 GO:0090092 GO:0090100 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:0140110 GO:1901360 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990904 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

255

Amino Acids

27.84

Weight (kDa)

8.79

Isoelectric Point (pI)

60.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 158 - 228 2.9e-10 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 60, 159, 208, 224, 706
AclWI GGATC 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 2 cut(s) 239, 259
AfiI CCNNNNNNNGG 3 cut(s) 59, 539, 740
AgsI TTSAA 2 cut(s) 482, 623
AjiI CACGTC 1 cut(s) 434
AjnI CCWGG 5 cut(s) 343, 427, 727, 734, 745
AluBI AGCT 4 cut(s) 272, 350, 403, 518
AluI AGCT 4 cut(s) 272, 350, 403, 518
AlwI GGATC 1 cut(s) 202
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 2 cut(s) 10, 425
ApeKI GCWGC 7 cut(s) 25, 28, 31, 34, 37, 638, 709
Asp700I GAANNNNTTC 2 cut(s) 441, 486
AspA2I CCTAGG 1 cut(s) 164
AspS9I GGNCC 6 cut(s) 50, 161, 308, 592, 731, 743
AvaII GGWCC 6 cut(s) 50, 161, 308, 592, 731, 743
AvrII CCTAGG 1 cut(s) 164
BauI CACGAG 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 348
BbvI GCAGC 7 cut(s) 37, 40, 43, 46, 49, 650, 696
BceAI ACGGC 2 cut(s) 25, 435
BcgI CGANNNNNNTGC 4 cut(s) 172, 206, 701, 735
BciT130I CCWGG 5 cut(s) 345, 429, 729, 736, 747
BciVI GTATCC 1 cut(s) 536
BclI TGATCA 1 cut(s) 322
BfaI CTAG 2 cut(s) 165, 423
BfmI CTRYAG 1 cut(s) 710
BfuI GTATCC 1 cut(s) 536
BisI GCNGC 9 cut(s) 26, 29, 32, 35, 38, 61, 639, 707, 710
BlnI CCTAGG 1 cut(s) 164
BlsI GCNGC 9 cut(s) 27, 30, 33, 36, 39, 62, 640, 708, 711
BmcAI AGTACT 1 cut(s) 259
Bme1390I CCNGG 5 cut(s) 345, 429, 729, 736, 747
Bme18I GGWCC 6 cut(s) 50, 161, 308, 592, 731, 743
BmgBI CACGTC 1 cut(s) 434
BmgT120I GGNCC 6 cut(s) 50, 161, 308, 592, 731, 743
BmiI GGNNCC 6 cut(s) 20, 52, 593, 594, 725, 733
BmrFI CCNGG 5 cut(s) 345, 429, 729, 736, 747
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 5 cut(s) 49, 114, 403, 448, 470
BmuI ACTGGG 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 3 cut(s) 267, 366, 672
BsaJI CCNNGG 3 cut(s) 164, 727, 734
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 539, 740
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 1 cut(s) 407
BseBI CCWGG 5 cut(s) 345, 429, 729, 736, 747
BseDI CCNNGG 3 cut(s) 164, 727, 734
BseGI GGATG 3 cut(s) 154, 461, 463
BseLI CCNNNNNNNGG 3 cut(s) 59, 539, 740
BseNI ACTGG 1 cut(s) 407
BseXI GCAGC 7 cut(s) 37, 40, 43, 46, 49, 650, 696
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 2 cut(s) 12, 427
BsiSI CCGG 1 cut(s) 22
BslFI GGGAC 4 cut(s) 20, 36, 605, 717
BslI CCNNNNNNNGG 3 cut(s) 59, 539, 740
BsmFI GGGAC 4 cut(s) 20, 36, 605, 717
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 2 cut(s) 12, 427
Bsp143I GATC 4 cut(s) 185, 194, 322, 361
BspACI CCGC 5 cut(s) 60, 159, 208, 224, 706
BspANI GGCC 2 cut(s) 12, 427
BspLI GGNNCC 6 cut(s) 20, 52, 593, 594, 725, 733
BspMAI CTGCAG 1 cut(s) 714
BspPI GGATC 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 21
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 3 cut(s) 164, 727, 734
BssMI GATC 4 cut(s) 185, 194, 322, 361
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 164
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 5 cut(s) 345, 429, 729, 736, 747
Bst4CI ACNGT 2 cut(s) 172, 442
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 1 cut(s) 65
BstF5I GGATG 3 cut(s) 154, 461, 463
BstKTI GATC 4 cut(s) 188, 197, 325, 364
BstMBI GATC 4 cut(s) 185, 194, 322, 361
BstMWI GCNNNNNNNGC 6 cut(s) 31, 34, 37, 638, 644, 653
BstNI CCWGG 5 cut(s) 345, 429, 729, 736, 747
BstSCI CCNGG 5 cut(s) 343, 427, 727, 734, 745
BstSFI CTRYAG 1 cut(s) 710
BstV1I GCAGC 7 cut(s) 37, 40, 43, 46, 49, 650, 696
BstV2I GAAGAC 1 cut(s) 348
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 2 cut(s) 12, 427
BtrI CACGTC 1 cut(s) 434
BtsCI GGATG 3 cut(s) 154, 461, 463
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 65
CaiI CAGNNNCTG 1 cut(s) 644
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 6 cut(s) 50, 161, 308, 592, 731, 743
Csp6I GTAC 2 cut(s) 238, 258
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 1 cut(s) 128
CviQI GTAC 2 cut(s) 238, 258
DpnI GATC 4 cut(s) 187, 196, 324, 363
DpnII GATC 4 cut(s) 185, 194, 322, 361
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 427
Eco47I GGWCC 6 cut(s) 50, 161, 308, 592, 731, 743
Eco57I CTGAAG 1 cut(s) 200
EcoO109I RGGNCCY 3 cut(s) 308, 592, 731
EcoRII CCWGG 5 cut(s) 343, 427, 727, 734, 745
EcoT14I CCWWGG 1 cut(s) 164
EcoT22I ATGCAT 2 cut(s) 129, 463
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 1 cut(s) 131
FaqI GGGAC 4 cut(s) 20, 36, 605, 717
FatI CATG 1 cut(s) 127
FauNDI CATATG 1 cut(s) 202
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 9 cut(s) 26, 29, 32, 35, 38, 61, 639, 707, 710
FokI GGATG 3 cut(s) 161, 448, 470
Fsp4HI GCNGC 9 cut(s) 26, 29, 32, 35, 38, 61, 639, 707, 710
FspBI CTAG 2 cut(s) 165, 423
GluI GCNGC 9 cut(s) 26, 29, 32, 35, 38, 61, 639, 707, 710
GsuI CTGGAG 3 cut(s) 267, 366, 672
HaeIII GGCC 2 cut(s) 12, 427
HapII CCGG 1 cut(s) 22
Hin1II CATG 1 cut(s) 131
HinfI GANTC 3 cut(s) 388, 572, 692
HpaII CCGG 1 cut(s) 22
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 4 cut(s) 85, 194, 219, 361
Hpy188III TCNNGA 6 cut(s) 119, 246, 455, 494, 689, 741
Hpy8I GTNNAC 1 cut(s) 240
HpyAV CCTTC 3 cut(s) 243, 276, 476
HpyCH4III ACNGT 2 cut(s) 172, 442
HpyCH4IV ACGT 3 cut(s) 90, 433, 751
HpyCH4V TGCA 8 cut(s) 67, 127, 140, 416, 461, 638, 661, 712
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 34, 37, 638, 644, 653
HpySE526I ACGT 3 cut(s) 90, 433, 751
Hsp92II CATG 1 cut(s) 131
KflI GGGWCCC 1 cut(s) 592
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 4 cut(s) 185, 194, 322, 361
LmnI GCTCC 4 cut(s) 269, 347, 408, 691
Lsp1109I GCAGC 7 cut(s) 37, 40, 43, 46, 49, 650, 696
LweI GCATC 5 cut(s) 49, 114, 403, 448, 470
MaeI CTAG 2 cut(s) 165, 423
MaeII ACGT 3 cut(s) 90, 433, 751
MaeIII GTNAC 1 cut(s) 752
MalI GATC 4 cut(s) 187, 196, 324, 363
MboI GATC 4 cut(s) 185, 194, 322, 361
MboII GAAGA 2 cut(s) 206, 348
MluCI AATT 1 cut(s) 697
MlyI GAGTC 1 cut(s) 581
Mph1103I ATGCAT 2 cut(s) 129, 463
MroXI GAANNNNTTC 2 cut(s) 441, 486
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 5 cut(s) 345, 429, 729, 736, 747
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 5 cut(s) 345, 429, 729, 736, 747
MwoI GCNNNNNNNGC 6 cut(s) 31, 34, 37, 638, 644, 653
NdeI CATATG 1 cut(s) 202
NdeII GATC 4 cut(s) 185, 194, 322, 361
NlaIII CATG 1 cut(s) 131
NlaIV GGNNCC 6 cut(s) 20, 52, 593, 594, 725, 733
NsiI ATGCAT 2 cut(s) 129, 463
PceI AGGCCT 1 cut(s) 427
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 2 cut(s) 441, 486
PfeI GAWTC 2 cut(s) 388, 692
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 9 cut(s) 27, 30, 33, 36, 39, 62, 640, 708, 711
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PpuMI RGGWCCY 3 cut(s) 308, 592, 731
Psp5II RGGWCCY 3 cut(s) 308, 592, 731
Psp6I CCWGG 5 cut(s) 343, 427, 727, 734, 745
PspGI CCWGG 5 cut(s) 343, 427, 727, 734, 745
PspN4I GGNNCC 6 cut(s) 20, 52, 593, 594, 725, 733
PspPI GGNCC 6 cut(s) 50, 161, 308, 592, 731, 743
PspPPI RGGWCCY 3 cut(s) 308, 592, 731
PstI CTGCAG 1 cut(s) 714
PstNI CAGNNNCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 239, 259
RsaNI GTAC 2 cut(s) 238, 258
RseI CAYNNNNRTG 1 cut(s) 585
SatI GCNGC 9 cut(s) 26, 29, 32, 35, 38, 61, 639, 707, 710
Sau3AI GATC 4 cut(s) 185, 194, 322, 361
Sau96I GGNCC 6 cut(s) 50, 161, 308, 592, 731, 743
ScaI AGTACT 1 cut(s) 259
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 5 cut(s) 345, 429, 729, 736, 747
SfaNI GCATC 5 cut(s) 49, 114, 403, 448, 470
SfcI CTRYAG 1 cut(s) 710
SinI GGWCC 6 cut(s) 50, 161, 308, 592, 731, 743
SmiMI CAYNNNNRTG 1 cut(s) 585
Sse9I AATT 1 cut(s) 697
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 5 cut(s) 60, 159, 208, 224, 706
SspMI CTAG 2 cut(s) 165, 423
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 5 cut(s) 343, 427, 727, 734, 745
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 2 cut(s) 172, 442
TaiI ACGT 3 cut(s) 93, 436, 754
TaqI TCGA 1 cut(s) 379
TasI AATT 1 cut(s) 697
TatI WGTACW 1 cut(s) 257
TauI GCSGC 2 cut(s) 63, 709
TfiI GAWTC 2 cut(s) 388, 692
TscAI CASTG 1 cut(s) 558
TseI GCWGC 7 cut(s) 25, 28, 31, 34, 37, 638, 709
TspDTI ATGAA 2 cut(s) 401, 684
TspRI CASTG 1 cut(s) 558
VpaK11BI GGWCC 6 cut(s) 50, 161, 308, 592, 731, 743
XmaJI CCTAGG 1 cut(s) 164
XmnI GAANNNNTTC 2 cut(s) 441, 486
XspI CTAG 2 cut(s) 165, 423
ZrmI AGTACT 1 cut(s) 259
Zsp2I ATGCAT 2 cut(s) 129, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.