pycom05g01200

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001021
Physical Location & Seq
Forward (+)
1514947 .. 1518953
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01200.3

Sequence Viewer

Length: 753 bp
ATGGGGGACGGCCTATGGAACCGGCAGCAGCAGCAGCAGCAGCAGCCGCAGCATCAACTGGGTCCCTCTGGCGGCGTGCATAAACGACCTCGTTCTGACTACGGTCAAGAGATGCATGGTTATTTTGCACGAGAAGATGATATTGGCGGTCCTAGGACTGTAAAGGATAGCAGATCAATTGGATCAGCATATGACCGCTATCTTCAGAATGCGGAAATGCCTTCGTACGCTTCTGCAGAAGTGAGTACTTTTGGTGGAGCTGGAATGGGAAGGGCTGTTGGTGGTGGAATGTCAGGTCCTCCAATGGCTGATCATTCTATGAGGGGTCGTCCTGGAGCTATGTCTTCTGATCTACTAACAAATGGTCGAAATATGAATCTTGCTAATCAGCTCCCAGTAGATGCAACGGCTAGGCCTGGACGTGAAACAGTTCCTTTACCTCTGGATGCATCCAGCACTTTATATGTTGAAGGACTTCCTCCTGACAGCACAAGGAGGGAAGTAGCTCATATCTTTCGTCCTTTTGTGGGATACAAAGCAGTGAGGCTTGTGAGCAAAGAGTCCAAACATCGTGGTGGGGACCCTCTTATTCTTTGTTTTGTGGATTTTGAAAATCCAGCCTGTGCAGCAACTGCTATGAGTGCCTTGCAAGGTTATGAAATGGATGAACACGCTCCAGATTCTAATTACTTGCGGCTGCAGTTTTCTCGGTTCCCAGGTCCCAGGTCTGGACCTGGACGTAACAAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000398 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003712 GO:0003713 GO:0003723 GO:0003727 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005685 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006979 GO:0008143 GO:0008150 GO:0008152 GO:0008380 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010494 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010862 GO:0016070 GO:0016071 GO:0017069 GO:0019219 GO:0019220 GO:0019222 GO:0023051 GO:0023056 GO:0030532 GO:0030619 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0034641 GO:0035613 GO:0035614 GO:0035770 GO:0036464 GO:0042325 GO:0042327 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0045937 GO:0046483 GO:0046983 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0060390 GO:0060391 GO:0060393 GO:0065007 GO:0070013 GO:0070717 GO:0071704 GO:0080090 GO:0090092 GO:0090100 GO:0090304 GO:0097159 GO:0097525 GO:0120114 GO:0140110 GO:1901360 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990904 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

251

Amino Acids

27.24

Weight (kDa)

8.9

Isoelectric Point (pI)

64.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 47, 72, 147, 196, 212, 694
AclWI GGATC 1 cut(s) 190
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 2 cut(s) 227, 247
AfiI CCNNNNNNNGG 3 cut(s) 71, 527, 728
AgsI TTSAA 2 cut(s) 470, 611
AjiI CACGTC 1 cut(s) 422
AjnI CCWGG 5 cut(s) 331, 415, 715, 722, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 4 cut(s) 260, 338, 391, 506
AluI AGCT 4 cut(s) 260, 338, 391, 506
AlwI GGATC 1 cut(s) 190
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 2 cut(s) 10, 413
Asp700I GAANNNNTTC 2 cut(s) 429, 474
AspA2I CCTAGG 1 cut(s) 152
AspS9I GGNCC 6 cut(s) 62, 149, 296, 580, 719, 731
AvaII GGWCC 6 cut(s) 62, 149, 296, 580, 719, 731
AvrII CCTAGG 1 cut(s) 152
BauI CACGAG 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 336
BceAI ACGGC 2 cut(s) 25, 423
BcgI CGANNNNNNTGC 2 cut(s) 689, 723
BciT130I CCWGG 5 cut(s) 333, 417, 717, 724, 735
BciVI GTATCC 1 cut(s) 524
BclI TGATCA 1 cut(s) 310
BfaI CTAG 2 cut(s) 153, 411
BfmI CTRYAG 2 cut(s) 234, 698
BfuI GTATCC 1 cut(s) 524
BlnI CCTAGG 1 cut(s) 152
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 5 cut(s) 333, 417, 717, 724, 735
Bme18I GGWCC 6 cut(s) 62, 149, 296, 580, 719, 731
BmgBI CACGTC 1 cut(s) 422
BmgT120I GGNCC 6 cut(s) 62, 149, 296, 580, 719, 731
BmiI GGNNCC 7 cut(s) 20, 63, 64, 581, 582, 713, 721
BmrFI CCNGG 5 cut(s) 333, 417, 717, 724, 735
BmrI ACTGGG 2 cut(s) 68, 389
BmsI GCATC 5 cut(s) 61, 102, 391, 436, 458
BmuI ACTGGG 2 cut(s) 68, 389
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 2 cut(s) 354, 660
BsaJI CCNNGG 3 cut(s) 152, 715, 722
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 527, 728
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 2 cut(s) 63, 395
BseBI CCWGG 5 cut(s) 333, 417, 717, 724, 735
BseDI CCNNGG 3 cut(s) 152, 715, 722
BseGI GGATG 3 cut(s) 449, 451, 670
BseLI CCNNNNNNNGG 3 cut(s) 71, 527, 728
BseNI ACTGG 2 cut(s) 63, 395
BsgI GTGCAG 1 cut(s) 645
BshFI GGCC 2 cut(s) 12, 415
BsiSI CCGG 1 cut(s) 22
BsiWI CGTACG 1 cut(s) 225
BslFI GGGAC 4 cut(s) 20, 48, 593, 705
BslI CCNNNNNNNGG 3 cut(s) 71, 527, 728
BsmFI GGGAC 4 cut(s) 20, 48, 593, 705
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 2 cut(s) 12, 415
Bsp143I GATC 4 cut(s) 173, 182, 310, 349
BspACI CCGC 6 cut(s) 47, 72, 147, 196, 212, 694
BspANI GGCC 2 cut(s) 12, 415
BspLI GGNNCC 7 cut(s) 20, 63, 64, 581, 582, 713, 721
BspMAI CTGCAG 2 cut(s) 238, 702
BspPI GGATC 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 21
BsrI ACTGG 2 cut(s) 63, 395
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 3 cut(s) 152, 715, 722
BssMI GATC 4 cut(s) 173, 182, 310, 349
BssSI CACGAG 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 129
Bst2UI CCWGG 5 cut(s) 333, 417, 717, 724, 735
Bst4CI ACNGT 3 cut(s) 104, 160, 430
BstAPI GCANNNNNTGC 1 cut(s) 632
BstC8I GCNNGC 1 cut(s) 77
BstF5I GGATG 3 cut(s) 449, 451, 670
BstKTI GATC 4 cut(s) 176, 185, 313, 352
BstMBI GATC 4 cut(s) 173, 182, 310, 349
BstNI CCWGG 5 cut(s) 333, 417, 717, 724, 735
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 5 cut(s) 331, 415, 715, 722, 733
BstSFI CTRYAG 2 cut(s) 234, 698
BstV2I GAAGAC 1 cut(s) 336
BsuI GTATCC 1 cut(s) 524
BsuRI GGCC 2 cut(s) 12, 415
BtrI CACGTC 1 cut(s) 422
BtsCI GGATG 3 cut(s) 449, 451, 670
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 546
Cac8I GCNNGC 1 cut(s) 77
CaiI CAGNNNCTG 1 cut(s) 632
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 6 cut(s) 62, 149, 296, 580, 719, 731
Csp6I GTAC 2 cut(s) 226, 246
CspCI CAANNNNNGTGG 2 cut(s) 553, 588
CviAII CATG 1 cut(s) 116
CviQI GTAC 2 cut(s) 226, 246
DpnI GATC 4 cut(s) 175, 184, 312, 351
DpnII GATC 4 cut(s) 173, 182, 310, 349
Eco130I CCWWGG 1 cut(s) 152
Eco147I AGGCCT 1 cut(s) 415
Eco47I GGWCC 6 cut(s) 62, 149, 296, 580, 719, 731
Eco57I CTGAAG 1 cut(s) 188
EcoO109I RGGNCCY 4 cut(s) 62, 296, 580, 719
EcoRII CCWGG 5 cut(s) 331, 415, 715, 722, 733
EcoT14I CCWWGG 1 cut(s) 152
EcoT22I ATGCAT 2 cut(s) 117, 451
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 1 cut(s) 119
FaqI GGGAC 4 cut(s) 20, 48, 593, 705
FatI CATG 1 cut(s) 115
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 310
FokI GGATG 3 cut(s) 436, 458, 677
FspBI CTAG 2 cut(s) 153, 411
GsuI CTGGAG 2 cut(s) 354, 660
HaeIII GGCC 2 cut(s) 12, 415
HapII CCGG 1 cut(s) 22
Hin1II CATG 1 cut(s) 119
HinfI GANTC 3 cut(s) 376, 560, 680
HpaII CCGG 1 cut(s) 22
Hpy188I TCNGA 3 cut(s) 97, 207, 349
Hpy188III TCNNGA 5 cut(s) 107, 443, 482, 677, 729
HpyAV CCTTC 3 cut(s) 231, 264, 464
HpyCH4III ACNGT 3 cut(s) 104, 160, 430
HpyCH4IV ACGT 2 cut(s) 421, 739
HpyCH4V TGCA 9 cut(s) 79, 115, 128, 236, 404, 449, 626, 649, 700
HpySE526I ACGT 2 cut(s) 421, 739
Hsp92II CATG 1 cut(s) 119
KflI GGGWCCC 2 cut(s) 62, 580
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 4 cut(s) 173, 182, 310, 349
LmnI GCTCC 4 cut(s) 257, 335, 396, 679
LweI GCATC 5 cut(s) 61, 102, 391, 436, 458
MaeI CTAG 2 cut(s) 153, 411
MaeII ACGT 2 cut(s) 421, 739
MaeIII GTNAC 1 cut(s) 740
MalI GATC 4 cut(s) 175, 184, 312, 351
MboI GATC 4 cut(s) 173, 182, 310, 349
MboII GAAGA 3 cut(s) 146, 194, 336
MfeI CAATTG 1 cut(s) 177
MluCI AATT 2 cut(s) 177, 685
MlyI GAGTC 1 cut(s) 569
MnlI CCTC 9 cut(s) 76, 99, 309, 315, 450, 489, 489, 537, 594
Mph1103I ATGCAT 2 cut(s) 117, 451
MroXI GAANNNNTTC 2 cut(s) 429, 474
MslI CAYNNNNRTG 1 cut(s) 573
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 5 cut(s) 333, 417, 717, 724, 735
MunI CAATTG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 5 cut(s) 333, 417, 717, 724, 735
NdeI CATATG 1 cut(s) 190
NdeII GATC 4 cut(s) 173, 182, 310, 349
NlaIII CATG 1 cut(s) 119
NlaIV GGNNCC 7 cut(s) 20, 63, 64, 581, 582, 713, 721
NsiI ATGCAT 2 cut(s) 117, 451
PceI AGGCCT 1 cut(s) 415
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 2 cut(s) 429, 474
PfeI GAWTC 2 cut(s) 376, 680
Pfl23II CGTACG 1 cut(s) 225
PfoI TCCNGGA 1 cut(s) 331
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
PpuMI RGGWCCY 4 cut(s) 62, 296, 580, 719
PshAI GACNNNNGTC 1 cut(s) 102
Psp5II RGGWCCY 4 cut(s) 62, 296, 580, 719
Psp6I CCWGG 5 cut(s) 331, 415, 715, 722, 733
PspGI CCWGG 5 cut(s) 331, 415, 715, 722, 733
PspLI CGTACG 1 cut(s) 225
PspN4I GGNNCC 7 cut(s) 20, 63, 64, 581, 582, 713, 721
PspPI GGNCC 6 cut(s) 62, 149, 296, 580, 719, 731
PspPPI RGGWCCY 4 cut(s) 62, 296, 580, 719
PstI CTGCAG 2 cut(s) 238, 702
PstNI CAGNNNCTG 1 cut(s) 632
RsaI GTAC 2 cut(s) 227, 247
RsaNI GTAC 2 cut(s) 226, 246
RseI CAYNNNNRTG 1 cut(s) 573
Sau3AI GATC 4 cut(s) 173, 182, 310, 349
Sau96I GGNCC 6 cut(s) 62, 149, 296, 580, 719, 731
ScaI AGTACT 1 cut(s) 247
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 5 cut(s) 333, 417, 717, 724, 735
SfaNI GCATC 5 cut(s) 61, 102, 391, 436, 458
SfcI CTRYAG 2 cut(s) 234, 698
SinI GGWCC 6 cut(s) 62, 149, 296, 580, 719, 731
SmiMI CAYNNNNRTG 1 cut(s) 573
Sse9I AATT 2 cut(s) 177, 685
SseBI AGGCCT 1 cut(s) 415
SsiI CCGC 6 cut(s) 47, 72, 147, 196, 212, 694
SspMI CTAG 2 cut(s) 153, 411
StuI AGGCCT 1 cut(s) 415
StyD4I CCNGG 5 cut(s) 331, 415, 715, 722, 733
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 3 cut(s) 104, 160, 430
TaiI ACGT 2 cut(s) 424, 742
TaqI TCGA 1 cut(s) 367
TasI AATT 2 cut(s) 177, 685
TatI WGTACW 1 cut(s) 245
TauI GCSGC 3 cut(s) 49, 75, 697
TfiI GAWTC 2 cut(s) 376, 680
TscAI CASTG 1 cut(s) 546
TspDTI ATGAA 3 cut(s) 389, 672, 681
TspRI CASTG 1 cut(s) 546
VpaK11BI GGWCC 6 cut(s) 62, 149, 296, 580, 719, 731
XmaJI CCTAGG 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 429, 474
XspI CTAG 2 cut(s) 153, 411
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 2 cut(s) 117, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.