Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002950

Physical Location & Seq

Genomic Coordinates Forward (+)

4120458 .. 4124987

Gene Structure 4530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1025300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGAAACGCTCAGCTGAGGCCGTTCCCATCGCCGCCTCCTCCGCCACCGCCTCCTCCTCGCTGCCGCCCAAGAAACCCGTTTTCTTAACCAAAGCCCAACGGGAGCAATTAGCCCTCCAACGCCGCCAAGAACAATCCGAAGACCAGAAACGCCACCAACACCATCTTCTCTCGAGTCTCTCCCACTCGCGCCCTTCCTCCGACAACGCACCGCCCGCAACCACCACTAGTTCTGACCGAGACAGAGATCGAGATAGAGACAGAGACCGAGATCATAGGGACCGAGACCGAGACAGGGATCGATACTCCCGTGACCGGGAGCGAGATTCGGAGCGGCGGAACCGCGACCGCGACCGAGATAGGGAGCGCGAAGAGGAGGCCAAGGCTAGAGAGCGTGCCCGCTTAGACAAATTGGCCGAGCGCGAGCGGGACAAAGAGCTGGAAGCCATTAAAGAGCAGTATCTTGGATCCAAGAAGCCCAAGAAGCGAGTGATCAAACCCAGCGAGAAGTTCCGATTTTCTTTCGATTGGGAGAACACAGAGGACACTTCTCGGGATATGAATGCTCTGTATCAAAACCCTCACGAGGCCCAGCTTCTGTTTGGGCGAGGGTTTCGGGCCGGGATGGACCGAAGGGAGCAGAAGAAGCTTGCTGTGAAGTACGAGAGGGAGCTGCGGGAGGAGATTCGGAAGAAGGACGGCGTCGAGGAGAGGCCTGAGGAGGCCGCTGCTCAGAGGCTTAAGGAGGAGGCTGCGGAAATGTATGACACTTTCGACATGAGGGTCGATCGCCATTGGACTGATAAGAAGCTTGAAGAGATAACTGAGAGGGACTGGAGGATTTTTCGAGAGGATTTTAACATTTCGTATAAGGGGTCAAGGATTCCGAGGCCAATGAGGAGTTGGGCAGAGAGTAAATTGAGTGAGGAGTTGCTTAAGACTGTGGAGAAGGCTGGGTACAAAAAGCCTTCTCCGATTCAGATGGCGGCGATTCCACTTGGCATGCAACAGCGCGATGTTATTGGCATTGCTGAGACTGGTTCTGGTAAGACTGCTGCCTTTGTTCTTCCTATGTTGACTTATATTTCGAGGTTGCCTCCCATTAGTGAGGAGAATGCGGCAGAAGGGCCTTATGCTGTTGTTATGGCGCCTACTCGTGAGCTTGCACAGCAGATTGAGGATGAGACTAACAAGTTTGCGCAATTCTTGGGTATCAAAGTGGTTTCCATTGTTGGTGGGCAGTCCATCGAGGATCAAGGGTTTAGAATTAGGCAAGGATGTGCAGTTGTAATTGCCACTCCAGGGCGTTTGATTGATTGCTTGGAGAGGCGTTATGCAGTTCTTAACCAGTGCAATTATGTTGTGCTTGATGAGGCTGATAGGATGATTGATATGGGGTTCGAGCCACAGGTTGTGGGAGTTTTAGATGCAATGCCCTCCAGCAATTTCAAACCTGAGAATGAGGACGAAGAACTTGATGAGAAGAAGATATACAGAACCACTTATATGTTCAGTGCCACCATGCCACCTGCTGTGGAGCGGCTTGCTAGGAAGTACTTGAGGAATCCTGTTGTGGTCACCATTGGCACGGCTGGAAAGACAACTGATTTGATCACTCAGAATGTGATCATGAGCAAGGAATCTGAGAAACTTGAGAGATTAAAGAGATTGCTTGACGAACTTGGTGATAAGACTGCTATTGTGTTCGTTAACACCAAGAAGAATGCTGACTATGTTGCTAAGAGTTTGGATAAGAATGGATATCGTGTTACCACTTTGCATGGAGGGAAGTCACAGGAGCAGAGAGAAATTAGCCTTGAAGGTTTTCGGACCAAGAAATACAATGTTCTAGTTGCAACCGATGTTGCAGGTCGTGGGATTGACATACCTGATGTGGCCTATGTTATTAATTACGATATGCCTGGGAATATCGAACAGTACACTCATCGTATTGGGCGTACTGGCCGTGCAGGGAAGACTGGTGTGGCCACAACATTCTTGACTATGCATGACACGGATGTATTTTATGATCTCAAGCAGATGCTTATTCAGAGTAATAGTCATGTTCCTCCTGAACTGGCGAAACATGAGGCCTCAAAGTTCAAGCCAGGTTCAGTTCCTGATAGACCACCAAGGCGTAATGATACTATTTTTACTCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0090304 GO:0097525 GO:0120114 GO:0140098 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

721

Amino Acids

82.86

Weight (kDa)

8.91

Isoelectric Point (pI)

44.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DDX23	PF25430	173 - 255	1.1e-22	DDX23-like domain
DEAD	PF00270	324 - 514	1.8e-45	DEAD/DEAH box helicase
Helicase_C	PF00271	549 - 658	3.3e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002950)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28180 AT1G28180 AT2G33730
fragaria_vesca	FvH4_1g07870 FvH4_2g13750
malus_domestica	MD05G1025300.v1.1 MD10G1025500.v1.1
prunus_persica	Prupe.8G029000_v2.0.a1 Prupe.8G029000_v2.0.a1
pyrus_communis	pycom05g01650 pycom10g01680
rosa_chinensis	RchiOBHm_Chr1g0333041 RchiOBHm_Chr2g0093751 RchiOBHm_Chr2g0093761 RchiOBHm_Chr6g0275441
rosa_laevigata	RLG00000013755 RLG00000016434
rosa_multiflora	Rmu_sc0004143.1_g000018 Rmu_sc0021925.1_g000003
rosa_roxburghii	Rroxscaffold_3G00237200 Rroxscaffold_7G00193300
rosa_rugosa	Rorug02G0039500 Rorug06G0092300 Rorug06G0092400 Rorug07G0206900
rosa_samantha	Rh1AG129900 Rh1CG123700 Rh1DG135400 Rh2BG086300 Rh2CG088700 Rh2DG084300 Rh2DG084400 Rh6AG205700 Rh6CG210900 Rh6DG201100 Rh7BG338800
rosa_wichuraiana	Rw1G010290 Rw6G017840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1537
AasI	GACNNNNNNGTC	1 cut(s)	782
Acc16I	TGCGCA	1 cut(s)	1200
Acc36I	ACCTGC	2 cut(s)	1537, 1859
AccB1I	GGYRCC	1 cut(s)	1147
AccBSI	CCGCTC	3 cut(s)	334, 427, 1540
AccII	CGCG	6 cut(s)	190, 345, 351, 369, 423, 1014
AclWI	GGATC	4 cut(s)	306, 462, 475, 1260
AcoI	YGGCCR	3 cut(s)	414, 1963, 1986
AcyI	GRCGYC	2 cut(s)	702, 1148
AfaI	GTAC	5 cut(s)	662, 959, 1556, 1940, 1960
AfiI	CCNNNNNNNGG	6 cut(s)	295, 315, 316, 361, 586, 1302
AflII	CTTAAG	2 cut(s)	740, 935
AgsI	TTSAA	4 cut(s)	815, 1450, 1820, 2104
AhlI	ACTAGT	1 cut(s)	227
AjnI	CCWGG	3 cut(s)	1300, 1921, 2107
AjuI	GAANNNNNNNTTGG	2 cut(s)	150, 182
AluBI	AGCT	7 cut(s)	14, 439, 595, 649, 673, 811, 1162
AluI	AGCT	7 cut(s)	14, 439, 595, 649, 673, 811, 1162
Alw26I	GTCTC	8 cut(s)	182, 234, 252, 258, 279, 285, 1028, 1178
AlwI	GGATC	4 cut(s)	306, 462, 475, 1260
AlwNI	CAGNNNCTG	2 cut(s)	598, 2120
Ama87I	CYCGRG	2 cut(s)	172, 552
ApeKI	GCWGC	5 cut(s)	61, 673, 728, 752, 1055
ArsI	GACNNNNNNTTYG	4 cut(s)	1069, 1101, 1591, 1623
AseI	ATTAAT	1 cut(s)	1908
Asp700I	GAANNNNTTC	1 cut(s)	1824
AspLEI	GCGC	6 cut(s)	192, 369, 423, 1014, 1150, 1201
AspS9I	GGNCC	6 cut(s)	280, 589, 618, 628, 1127, 1830
AsuC2I	CCSGG	2 cut(s)	317, 622
AsuHPI	GGTGA	2 cut(s)	1570, 1697
AvaI	CYCGRG	2 cut(s)	172, 552
AvaII	GGWCC	3 cut(s)	280, 628, 1830
AxyI	CCTNAGG	1 cut(s)	717
BaeGI	GKGCMC	1 cut(s)	400
BaeI	ACNNNNGTAYC	2 cut(s)	295, 328
BalI	TGGCCA	1 cut(s)	1988
BamHI	GGATCC	1 cut(s)	467
BanI	GGYRCC	1 cut(s)	1147
BarI	GAAGNNNNNNTAC	4 cut(s)	941, 973, 1475, 1507
BauI	CACGAG	2 cut(s)	584, 1155
BbsI	GAAGAC	2 cut(s)	147, 1982
BbvCI	CCTCAGC	1 cut(s)	15
BbvI	GCAGC	5 cut(s)	48, 660, 715, 739, 1042
BccI	CCATC	5 cut(s)	35, 171, 619, 976, 1253
BceAI	ACGGC	4 cut(s)	5, 715, 1605, 1950
BciT130I	CCWGG	3 cut(s)	1302, 1923, 2109
BclI	TGATCA	3 cut(s)	492, 1611, 1626
BcnI	CCSGG	2 cut(s)	317, 622
BcoDI	GTCTC	8 cut(s)	182, 234, 252, 258, 279, 285, 1028, 1178
BcuI	ACTAGT	1 cut(s)	227
BfaI	CTAG	4 cut(s)	228, 387, 1548, 1850
BfoI	RGCGCY	1 cut(s)	1151
BfrI	CTTAAG	2 cut(s)	740, 935
BfuAI	ACCTGC	2 cut(s)	1537, 1859
BlpI	GCTNAGC	1 cut(s)	10
BmcAI	AGTACT	1 cut(s)	1556
Bme1390I	CCNGG	5 cut(s)	317, 622, 1302, 1923, 2109
Bme18I	GGWCC	3 cut(s)	280, 628, 1830
BmeT110I	CYCGRG	2 cut(s)	172, 552
BmgT120I	GGNCC	6 cut(s)	280, 589, 618, 628, 1127, 1830
BmiI	GGNNCC	4 cut(s)	281, 341, 469, 1149
BmrFI	CCNGG	5 cut(s)	317, 622, 1302, 1923, 2109
BmsI	GCATC	2 cut(s)	1417, 2031
BpiI	GAAGAC	2 cut(s)	147, 1982
BplI	GAGNNNNNCTC	2 cut(s)	1081, 1113
BpmI	CTGGAG	3 cut(s)	856, 1284, 1423
Bpu10I	CCTNAGC	1 cut(s)	15
Bpu1102I	GCTNAGC	1 cut(s)	10
BpuEI	CTTGAG	3 cut(s)	1579, 1673, 2018
BpuMI	CCSGG	2 cut(s)	317, 622
Bsa29I	ATCGAT	1 cut(s)	301
BsaHI	GRCGYC	2 cut(s)	702, 1148
BsaI	GGTCTC	2 cut(s)	258, 279
BsaJI	CCNNGG	5 cut(s)	381, 887, 1301, 1922, 2132
Bsc4I	CCNNNNNNNGG	6 cut(s)	295, 315, 316, 361, 586, 1302
Bse1I	ACTGG	6 cut(s)	839, 1042, 1348, 1966, 1984, 2082
Bse21I	CCTNAGG	1 cut(s)	717
Bse3DI	GCAATG	2 cut(s)	1026, 1437
BseBI	CCWGG	3 cut(s)	1302, 1923, 2109
BseCI	ATCGAT	1 cut(s)	301
BseDI	CCNNGG	5 cut(s)	381, 887, 1301, 1922, 2132
BseGI	GGATG	5 cut(s)	630, 1186, 1283, 1389, 2023
BseLI	CCNNNNNNNGG	6 cut(s)	295, 315, 316, 361, 586, 1302
BseMI	GCAATG	2 cut(s)	1026, 1437
BseMII	CTCAG	9 cut(s)	6, 24, 708, 746, 816, 1023, 1446, 1631, 1635
BseNI	ACTGG	6 cut(s)	839, 1042, 1348, 1966, 1984, 2082
BseSI	GKGCMC	1 cut(s)	400
BseXI	GCAGC	5 cut(s)	48, 660, 715, 739, 1042
BseYI	CCCAGC	3 cut(s)	500, 591, 953
BsgI	GTGCAG	2 cut(s)	1302, 1989
Bsh1236I	CGCG	6 cut(s)	190, 345, 351, 369, 423, 1014
Bsh1285I	CGRYCG	3 cut(s)	349, 355, 790
BshNI	GGYRCC	1 cut(s)	1147
BshVI	ATCGAT	1 cut(s)	301
BsiEI	CGRYCG	3 cut(s)	349, 355, 790
BsiHKCI	CYCGRG	2 cut(s)	172, 552
BsiSI	CCGG	2 cut(s)	316, 621
BslFI	GGGAC	3 cut(s)	293, 443, 845
BslI	CCNNNNNNNGG	6 cut(s)	295, 315, 316, 361, 586, 1302
BsmAI	GTCTC	8 cut(s)	182, 234, 252, 258, 279, 285, 1028, 1178
BsmFI	GGGAC	3 cut(s)	293, 443, 845
BsmI	GAATGC	3 cut(s)	568, 1120, 1729
Bso31I	GGTCTC	2 cut(s)	258, 279
BsoBI	CYCGRG	2 cut(s)	172, 552
Bsp1286I	GDGCHC	1 cut(s)	400
Bsp1720I	GCTNAGC	1 cut(s)	10
BspCNI	CTCAG	9 cut(s)	7, 23, 709, 745, 817, 1024, 1447, 1630, 1636
BspDI	ATCGAT	1 cut(s)	301
BspFNI	CGCG	6 cut(s)	190, 345, 351, 369, 423, 1014
BspHI	TCATGA	1 cut(s)	1629
BspLI	GGNNCC	4 cut(s)	281, 341, 469, 1149
BspMI	ACCTGC	2 cut(s)	1537, 1859
BspPI	GGATC	4 cut(s)	306, 462, 475, 1260
BspT107I	GGYRCC	1 cut(s)	1147
BspTI	CTTAAG	2 cut(s)	740, 935
BspTNI	GGTCTC	2 cut(s)	258, 279
BsrBI	CCGCTC	3 cut(s)	334, 427, 1540
BsrDI	GCAATG	2 cut(s)	1026, 1437
BsrI	ACTGG	6 cut(s)	839, 1042, 1348, 1966, 1984, 2082
BssECI	CCNNGG	5 cut(s)	381, 887, 1301, 1922, 2132
BssNI	GRCGYC	2 cut(s)	702, 1148
BssSI	CACGAG	2 cut(s)	584, 1155
BssT1I	CCWWGG	2 cut(s)	381, 2132
Bst2BI	CACGAG	2 cut(s)	584, 1155
Bst2UI	CCWGG	3 cut(s)	1302, 1923, 2109
Bst4CI	ACNGT	2 cut(s)	943, 1938
Bst6I	CTCTTC	2 cut(s)	366, 810
BstACI	GRCGYC	2 cut(s)	702, 1148
BstAFI	CTTAAG	2 cut(s)	740, 935
BstC8I	GCNNGC	8 cut(s)	216, 396, 400, 425, 651, 1004, 1164, 1545
BstEII	GGTNACC	1 cut(s)	1576
BstF5I	GGATG	5 cut(s)	630, 1186, 1283, 1389, 2023
BstFNI	CGCG	6 cut(s)	190, 345, 351, 369, 423, 1014
BstH2I	RGCGCY	1 cut(s)	1151
BstHHI	GCGC	6 cut(s)	192, 369, 423, 1014, 1150, 1201
BstMAI	GTCTC	8 cut(s)	182, 234, 252, 258, 279, 285, 1028, 1178
BstMCI	CGRYCG	3 cut(s)	349, 355, 790
BstMWI	GCNNNNNNNGC	5 cut(s)	41, 215, 484, 646, 1168
BstNI	CCWGG	3 cut(s)	1302, 1923, 2109
BstNSI	RCATGY	1 cut(s)	1006
BstPI	GGTNACC	1 cut(s)	1576
BstSCI	CCNGG	5 cut(s)	315, 620, 1300, 1921, 2107
BstSLI	GKGCMC	1 cut(s)	400
BstUI	CGCG	6 cut(s)	190, 345, 351, 369, 423, 1014
BstV1I	GCAGC	5 cut(s)	48, 660, 715, 739, 1042
BstV2I	GAAGAC	2 cut(s)	147, 1982
BstX2I	RGATCY	1 cut(s)	467
BstYI	RGATCY	1 cut(s)	467
Bsu15I	ATCGAT	1 cut(s)	301
Bsu36I	CCTNAGG	1 cut(s)	717
BsuTUI	ATCGAT	1 cut(s)	301
BtgZI	GCGATG	2 cut(s)	13, 1029
BtsCI	GGATG	5 cut(s)	630, 1186, 1283, 1389, 2023
BtsIMutI	CAGTG	3 cut(s)	1355, 1519, 2158
BveI	ACCTGC	2 cut(s)	1537, 1859
Cac8I	GCNNGC	8 cut(s)	216, 396, 400, 425, 651, 1004, 1164, 1545
CaiI	CAGNNNCTG	2 cut(s)	598, 2120
CciI	TCATGA	1 cut(s)	1629
CfoI	GCGC	6 cut(s)	192, 369, 423, 1014, 1150, 1201
Cfr13I	GGNCC	6 cut(s)	280, 589, 618, 628, 1127, 1830
ClaI	ATCGAT	1 cut(s)	301
CseI	GACGC	1 cut(s)	691
Csp6I	GTAC	5 cut(s)	661, 958, 1555, 1939, 1959
CviAII	CATG	8 cut(s)	778, 1003, 1522, 1630, 1781, 2009, 2063, 2087
CviQI	GTAC	5 cut(s)	661, 958, 1555, 1939, 1959
DinI	GGCGCC	1 cut(s)	1149
DrdI	GACNNNNNNGTC	1 cut(s)	782
DseDI	GACNNNNNNGTC	1 cut(s)	782
EaeI	YGGCCR	3 cut(s)	414, 1963, 1986
Eam1104I	CTCTTC	2 cut(s)	366, 810
EarI	CTCTTC	2 cut(s)	366, 810
EciI	GGCGGA	2 cut(s)	31, 352
Eco130I	CCWWGG	2 cut(s)	381, 2132
Eco147I	AGGCCT	2 cut(s)	715, 2093
Eco31I	GGTCTC	2 cut(s)	258, 279
Eco32I	GATATC	1 cut(s)	1763
Eco47I	GGWCC	3 cut(s)	280, 628, 1830
Eco81I	CCTNAGG	1 cut(s)	717
Eco88I	CYCGRG	2 cut(s)	172, 552
Eco91I	GGTNACC	1 cut(s)	1576
EcoO109I	RGGNCCY	1 cut(s)	1127
EcoO65I	GGTNACC	1 cut(s)	1576
EcoRII	CCWGG	3 cut(s)	1300, 1921, 2107
EcoRV	GATATC	1 cut(s)	1763
EcoT14I	CCWWGG	2 cut(s)	381, 2132
EcoT22I	ATGCAT	1 cut(s)	2010
EgeI	GGCGCC	1 cut(s)	1149
EheI	GGCGCC	1 cut(s)	1149
ErhI	CCWWGG	2 cut(s)	381, 2132
FaeI	CATG	8 cut(s)	781, 1006, 1525, 1633, 1784, 2012, 2066, 2090
FaqI	GGGAC	3 cut(s)	293, 443, 845
FatI	CATG	8 cut(s)	777, 1002, 1521, 1629, 1780, 2008, 2062, 2086
FauI	CCCGC	4 cut(s)	223, 407, 420, 669
FbaI	TGATCA	3 cut(s)	492, 1611, 1626
FokI	GGATG	5 cut(s)	637, 1193, 1290, 1396, 2030
FspBI	CTAG	4 cut(s)	228, 387, 1548, 1850
FspI	TGCGCA	1 cut(s)	1200
GlaI	GCGC	6 cut(s)	191, 368, 422, 1013, 1149, 1200
GsaI	CCCAGC	3 cut(s)	504, 595, 957
GsuI	CTGGAG	3 cut(s)	856, 1284, 1423
HaeII	RGCGCY	1 cut(s)	1151
HapII	CCGG	2 cut(s)	316, 621
HgaI	GACGC	1 cut(s)	691
HhaI	GCGC	6 cut(s)	192, 369, 423, 1014, 1150, 1201
Hin1I	GRCGYC	2 cut(s)	702, 1148
Hin1II	CATG	8 cut(s)	781, 1006, 1525, 1633, 1784, 2012, 2066, 2090
Hin6I	GCGC	6 cut(s)	190, 367, 421, 1012, 1148, 1199
HinP1I	GCGC	6 cut(s)	190, 367, 421, 1012, 1148, 1199
HincII	GTYRAC	2 cut(s)	1077, 1711
HindII	GTYRAC	2 cut(s)	1077, 1711
HindIII	AAGCTT	2 cut(s)	647, 809
HinfI	GANTC	8 cut(s)	175, 326, 685, 883, 976, 991, 1564, 1640
HpaI	GTTAAC	1 cut(s)	1711
HpaII	CCGG	2 cut(s)	316, 621
HphI	GGTGA	2 cut(s)	1570, 1697
Hpy166II	GTNNAC	3 cut(s)	1077, 1711, 1941
Hpy8I	GTNNAC	3 cut(s)	1077, 1711, 1941
Hpy99I	CGWCG	1 cut(s)	707
HpyAV	CCTTC	7 cut(s)	204, 627, 688, 943, 978, 1118, 1814
HpyCH4III	ACNGT	2 cut(s)	943, 1938
HpyF10VI	GCNNNNNNNGC	5 cut(s)	41, 215, 484, 646, 1168
Hsp92I	GRCGYC	2 cut(s)	702, 1148
Hsp92II	CATG	8 cut(s)	781, 1006, 1525, 1633, 1784, 2012, 2066, 2090
HspAI	GCGC	6 cut(s)	190, 367, 421, 1012, 1148, 1199
KasI	GGCGCC	1 cut(s)	1147
Ksp22I	TGATCA	3 cut(s)	492, 1611, 1626
KspAI	GTTAAC	1 cut(s)	1711
LmnI	GCTCC	8 cut(s)	103, 319, 331, 364, 637, 670, 1537, 1798
Lsp1109I	GCAGC	5 cut(s)	48, 660, 715, 739, 1042
LweI	GCATC	2 cut(s)	1417, 2031
MaeI	CTAG	4 cut(s)	228, 387, 1548, 1850
MaeIII	GTNAC	4 cut(s)	311, 1576, 1768, 1791
MbiI	CCGCTC	3 cut(s)	334, 427, 1540
MflI	RGATCY	1 cut(s)	467
MhlI	GDGCHC	1 cut(s)	400
MlsI	TGGCCA	1 cut(s)	1988
MluNI	TGGCCA	1 cut(s)	1988
Mly113I	GGCGCC	1 cut(s)	1148
MlyI	GAGTC	1 cut(s)	184
MmeI	TCCRAC	2 cut(s)	142, 225
Mox20I	TGGCCA	1 cut(s)	1988
Mph1103I	ATGCAT	1 cut(s)	2010
MroXI	GAANNNNTTC	1 cut(s)	1824
MscI	TGGCCA	1 cut(s)	1988
MseI	TTAA	9 cut(s)	86, 450, 741, 858, 936, 1344, 1661, 1710, 1908
MslI	CAYNNNNRTG	1 cut(s)	1505
Msp20I	TGGCCA	1 cut(s)	1988
MspA1I	CMGCKG	2 cut(s)	14, 728
MspCI	CTTAAG	2 cut(s)	740, 935
MspI	CCGG	2 cut(s)	316, 621
MspR9I	CCNGG	5 cut(s)	317, 622, 1302, 1923, 2109
Mva1269I	GAATGC	3 cut(s)	568, 1120, 1729
MvaI	CCWGG	3 cut(s)	1302, 1923, 2109
MvnI	CGCG	6 cut(s)	190, 345, 351, 369, 423, 1014
MwoI	GCNNNNNNNGC	5 cut(s)	41, 215, 484, 646, 1168
NarI	GGCGCC	1 cut(s)	1148
NciI	CCSGG	2 cut(s)	317, 622
NlaIII	CATG	8 cut(s)	781, 1006, 1525, 1633, 1784, 2012, 2066, 2090
NlaIV	GGNNCC	4 cut(s)	281, 341, 469, 1149
NmeAIII	GCCGAG	1 cut(s)	442
NmuCI	GTSAC	3 cut(s)	311, 1576, 1791
NsbI	TGCGCA	1 cut(s)	1200
NsiI	ATGCAT	1 cut(s)	2010
NspI	RCATGY	1 cut(s)	1006
PaeI	GCATGC	1 cut(s)	1006
PaeR7I	CTCGAG	1 cut(s)	172
PagI	TCATGA	1 cut(s)	1629
PaqCI	CACCTGC	1 cut(s)	1537
PceI	AGGCCT	2 cut(s)	715, 2093
PcsI	WCGNNNNNNNCGW	2 cut(s)	307, 1954
PctI	GAATGC	3 cut(s)	568, 1120, 1729
PdmI	GAANNNNTTC	1 cut(s)	1824
PfeI	GAWTC	7 cut(s)	326, 685, 883, 976, 991, 1564, 1640
PflFI	GACNNNGTC	1 cut(s)	701
Ple19I	CGATCG	1 cut(s)	790
PleI	GAGTC	1 cut(s)	183
PluTI	GGCGCC	1 cut(s)	1151
PpsI	GAGTC	1 cut(s)	183
PshBI	ATTAAT	1 cut(s)	1908
Psp6I	CCWGG	3 cut(s)	1300, 1921, 2107
PspEI	GGTNACC	1 cut(s)	1576
PspFI	CCCAGC	3 cut(s)	500, 591, 953
PspGI	CCWGG	3 cut(s)	1300, 1921, 2107
PspN4I	GGNNCC	4 cut(s)	281, 341, 469, 1149
PspPI	GGNCC	6 cut(s)	280, 589, 618, 628, 1127, 1830
PstNI	CAGNNNCTG	2 cut(s)	598, 2120
PsuI	RGATCY	1 cut(s)	467
PsyI	GACNNNGTC	1 cut(s)	701
PvuI	CGATCG	1 cut(s)	790
PvuII	CAGCTG	1 cut(s)	14
RsaI	GTAC	5 cut(s)	662, 959, 1556, 1940, 1960
RsaNI	GTAC	5 cut(s)	661, 958, 1555, 1939, 1959
RseI	CAYNNNNRTG	1 cut(s)	1505
SaqAI	TTAA	9 cut(s)	86, 450, 741, 858, 936, 1344, 1661, 1710, 1908
Sau96I	GGNCC	6 cut(s)	280, 589, 618, 628, 1127, 1830
ScaI	AGTACT	1 cut(s)	1556
SchI	GAGTC	1 cut(s)	184
ScrFI	CCNGG	5 cut(s)	317, 622, 1302, 1923, 2109
SduI	GDGCHC	1 cut(s)	400
SfaNI	GCATC	2 cut(s)	1417, 2031
SfoI	GGCGCC	1 cut(s)	1149
Sfr274I	CTCGAG	1 cut(s)	172
SinI	GGWCC	3 cut(s)	280, 628, 1830
SlaI	CTCGAG	1 cut(s)	172
SmiMI	CAYNNNNRTG	1 cut(s)	1505
SmlI	CTYRAG	6 cut(s)	172, 740, 935, 1558, 1652, 2033
SmoI	CTYRAG	6 cut(s)	172, 740, 935, 1558, 1652, 2033
SpeI	ACTAGT	1 cut(s)	227
SphI	GCATGC	1 cut(s)	1006
SseBI	AGGCCT	2 cut(s)	715, 2093
SspDI	GGCGCC	1 cut(s)	1147
SspMI	CTAG	4 cut(s)	228, 387, 1548, 1850
StuI	AGGCCT	2 cut(s)	715, 2093
StyD4I	CCNGG	5 cut(s)	315, 620, 1300, 1921, 2107
StyI	CCWWGG	2 cut(s)	381, 2132
TaaI	ACNGT	2 cut(s)	943, 1938
TaqII	GACCGA	6 cut(s)	252, 282, 297, 303, 369, 645
TatI	WGTACW	2 cut(s)	1554, 1938
TauI	GCSGC	8 cut(s)	35, 67, 126, 337, 728, 989, 1121, 1543
TfiI	GAWTC	7 cut(s)	326, 685, 883, 976, 991, 1564, 1640
Tru1I	TTAA	9 cut(s)	86, 450, 741, 858, 936, 1344, 1661, 1710, 1908
Tru9I	TTAA	9 cut(s)	86, 450, 741, 858, 936, 1344, 1661, 1710, 1908
TscAI	CASTG	2 cut(s)	1355, 1519
TseFI	GTSAC	3 cut(s)	311, 1576, 1791
TseI	GCWGC	5 cut(s)	61, 673, 728, 752, 1055
Tsp45I	GTSAC	3 cut(s)	311, 1576, 1791
TspDTI	ATGAA	2 cut(s)	17, 575
TspGWI	ACGGA	1 cut(s)	2030
TspRI	CASTG	2 cut(s)	1355, 1519
Tth111I	GACNNNGTC	1 cut(s)	701
Vha464I	CTTAAG	2 cut(s)	740, 935
VpaK11BI	GGWCC	3 cut(s)	280, 628, 1830
VspI	ATTAAT	1 cut(s)	1908
XceI	RCATGY	1 cut(s)	1006
XcmI	CCANNNNNNNNNTGG	2 cut(s)	599, 900
XhoI	CTCGAG	1 cut(s)	172
XmnI	GAANNNNTTC	1 cut(s)	1824
XspI	CTAG	4 cut(s)	228, 387, 1548, 1850
ZrmI	AGTACT	1 cut(s)	1556
Zsp2I	ATGCAT	1 cut(s)	2010

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.