Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002950

Physical Location & Seq

Genomic Coordinates Forward (+)

3182332 .. 3185447

Gene Structure 3116 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1025500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2181 bp

ATGAAACGCTCAGCCGATGGCGTTCCCATCGCCGCTTCCTCCGCCACTGCCTCCTCCTCGCTGCCGCCCAAGAAACCTGTTTTCTTAACTAAAGCCCAACGCGAGCAATTAGCCCTCCAACGCCGTCAAGAACAAACCGAAGATCAAAAGCGCCACCAGAACCATCTCCTCTCCAGTCTCTCCCACTCGCGCCCTTCATCCGACGCTGCACCGCCCGTAACCACCCCCAATTCTGACCGAGACCGAGATCGAGACAGAGACAGAGACCGAGATCATAGGGACCGAGAGCGGGACAGAGACAGGGACCGATACTCCCGTGACCGGGAACGAGACCGGGAACGAGATTCCGAGCGGCGGAACCGAGACCGAGACCGAGATAGGGAGCGGGAAGAGGAGGCCAAGGCTCGAGAGCGTGCCCGCTTAGACAAATTGGCGGAGCGCGAGCGGGACAAAGAGCTGGAAGCCATTAAAGAGCAGTATCTCGGATCGAAGAAGCCCAAGAAGCGAGTCATCAAACCCAGCGAGAAATTCCGATTCTCTTTCGATTGGGAGAACACGGAGGACACCTCTCGGGATATGAATGCTTTGTACCAAAACCCTCATGAGGCCCAGCTTTTGTTTGGGCGAGGGTTTCGGGCCGGGATGGACAGAAGGGAGCAGAAGAAGCTTGCTGTAAAGTACGAGAGGGAGCTGCGTGAGGAGATTCGGAAGAAGGACGGCGTAGAGGAGAGGCCTGAGGAGGCCGCTGCGCAGAGGCTCAAGGAGGAGGCTGCGGAAATGTATGACACTTTTGACATGAGGGTTGATCGCCATTGGACTGATAAGAAGCTTGAAGAGATGACTGAGAGGGATTGGAGGATTTTTAGGGAGGATTTTAACATTTCGTACAAGGGGTCGAGGATTCCGAGGCCAATGAGGAGTTGGGCTGAGAGTAAATTGAGTGAGGAGTTGCTTAAGACTGTGGAGAAAGCTGGGTACAAAAAGCCTTCTCCTATTCAGATGGCGGCGATTCCACTTGGGATGCAACAGCGCGATGTTATTGGCATTGCTGAGACTGGTTCGGGTAAGACTGCTGCCTTTGTTCTTCCTATGTTGACTTATATTTCGAGGTTGCCTCCCATTAGTGAGGAGAATGCGGCCGAAGGGCCTTATGCTGTTGTTATGGCGCCTACTCGTGAGCTTGCACAACAGATTGAGGATGAGACTAACAAGTTTGCGCAATACTTGGGTATCAAAGTGGTTTCCATTGTTGGCGGGCAGTCAATCGAGGATCAAGGTTTTAGAATTAGGCAAGGCTGTGCGGTTGTAATTGCTACTCCAGGGCGTTTGATTGATTGCTTGGAGAGGCGTTATGCTGTTCTTAACCAGTGCAATTATGTTGTTCTTGATGAGGCTGATAGGATGATTGATATGGGGTTTGAGCCACAGGTTGTCGGTGTTTTAGATGCAATGCCCTCCAGCAATTTCAAACCTGAGAATGAGGATGAAGAACTTGATGAGAAGAAGATTTATAGAACCACTTATATGTTCAGTGCCACCATGCCCCCTGCTGTGGAGCGGCTTGCTAGGAAGTACTTGAGGAATCCTGTTGTGGTCACAATTGGAACAGCTGGAAAGACAACTGATTTAATTACTCAGAATGTAATCATGACCAAGGAATCAGAAAAGTTTGAGAGGTTAAAGAGATTGCTTGACGAACTTGGTGATAAGACTGCTATTGTGTTCGTTAACACCAAGAAGAATGCAGACTACGTTGCTAAGAGTTTGGATAAAAATGGATATCGTGTGACCACTTTGCATGGAGGGAAGTCACAGGAGCAGAGAGAAATTAGCCTTGAAGGTTTTCGGACCAAGAAATACAATGTTCTAGTTGCGACCGATGTTGCAGGTCGTGGGATTGACATACCTGATGTGGCCTATGTTATTAATTACGATACGCCTGGGAATATCGAACAGTACACTCATCGTATTGGGCGTACTGGCCGTGCAGGAAAGACTGGTGTGGCCACAACATTCTTGACTATGCACGACACAGATGTATTTTATGATCTCAAGCAGATGCTTATTCAGAGTAATAGTCATGTTCCTCCTGAACTGGCGAAACATGAGGCCTCAAAGTTCAAGCCAGGTTCAGTGCCTGATAGACCACCAAGGCGTAATGATACTATTTTTACTCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0090304 GO:0097525 GO:0120114 GO:0140098 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

727

Amino Acids

83.72

Weight (kDa)

8.91

Isoelectric Point (pI)

41.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DDX23	PF25430	179 - 261	1.1e-22	DDX23-like domain
DEAD	PF00270	330 - 520	1.7e-45	DEAD/DEAH box helicase
Helicase_C	PF00271	555 - 664	6.9e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002950)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28180 AT1G28180 AT2G33730
fragaria_vesca	FvH4_1g07870 FvH4_2g13750
malus_domestica	MD05G1025300.v1.1 MD10G1025500.v1.1
prunus_persica	Prupe.8G029000_v2.0.a1 Prupe.8G029000_v2.0.a1
pyrus_communis	pycom05g01650 pycom10g01680
rosa_chinensis	RchiOBHm_Chr1g0333041 RchiOBHm_Chr2g0093751 RchiOBHm_Chr2g0093761 RchiOBHm_Chr6g0275441
rosa_laevigata	RLG00000013755 RLG00000016434
rosa_multiflora	Rmu_sc0004143.1_g000018 Rmu_sc0021925.1_g000003
rosa_roxburghii	Rroxscaffold_3G00237200 Rroxscaffold_7G00193300
rosa_rugosa	Rorug02G0039500 Rorug06G0092300 Rorug06G0092400 Rorug07G0206900
rosa_samantha	Rh1AG129900 Rh1CG123700 Rh1DG135400 Rh2BG086300 Rh2CG088700 Rh2DG084300 Rh2DG084400 Rh6AG205700 Rh6CG210900 Rh6DG201100 Rh7BG338800
rosa_wichuraiana	Rw1G010290 Rw6G017840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	750, 1218
Acc36I	ACCTGC	1 cut(s)	1877
AccB1I	GGYRCC	1 cut(s)	1165
AccBSI	CCGCTC	5 cut(s)	289, 352, 385, 445, 1558
AccII	CGCG	4 cut(s)	102, 190, 441, 1032
AclWI	GGATC	2 cut(s)	493, 1278
AcoI	YGGCCR	3 cut(s)	1137, 1981, 2004
AcsI	RAATTY	1 cut(s)	527
AcyI	GRCGYC	1 cut(s)	1166
AfaI	GTAC	7 cut(s)	590, 680, 887, 977, 1574, 1958, 1978
AfiI	CCNNNNNNNGG	7 cut(s)	289, 321, 322, 354, 379, 604, 1552
AflII	CTTAAG	1 cut(s)	953
AgsI	TTSAA	4 cut(s)	833, 1468, 1838, 2122
AjnI	CCWGG	3 cut(s)	1318, 1939, 2125
AluBI	AGCT	8 cut(s)	457, 613, 667, 691, 829, 971, 1180, 1610
AluI	AGCT	8 cut(s)	457, 613, 667, 691, 829, 971, 1180, 1610
AlwI	GGATC	2 cut(s)	493, 1278
AlwNI	CAGNNNCTG	1 cut(s)	2138
Ama87I	CYCGRG	2 cut(s)	405, 570
ApeKI	GCWGC	6 cut(s)	61, 206, 691, 746, 770, 1073
ApoI	RAATTY	1 cut(s)	527
ArsI	GACNNNNNNTTYG	2 cut(s)	1087, 1119
AseI	ATTAAT	1 cut(s)	1926
Asp700I	GAANNNNTTC	1 cut(s)	1842
AspLEI	GCGC	7 cut(s)	153, 192, 441, 751, 1032, 1168, 1219
AspS9I	GGNCC	6 cut(s)	280, 304, 607, 636, 1145, 1848
AsuC2I	CCSGG	3 cut(s)	323, 335, 640
AsuHPI	GGTGA	1 cut(s)	1715
AvaI	CYCGRG	2 cut(s)	405, 570
AvaII	GGWCC	3 cut(s)	280, 304, 1848
AxyI	CCTNAGG	1 cut(s)	735
BaeGI	GKGCMC	1 cut(s)	418
BaeI	ACNNNNGTAYC	4 cut(s)	301, 334, 1213, 1246
BalI	TGGCCA	1 cut(s)	2006
BanI	GGYRCC	1 cut(s)	1165
BauI	CACGAG	1 cut(s)	1173
BbvI	GCAGC	6 cut(s)	48, 193, 678, 733, 757, 1060
BccI	CCATC	5 cut(s)	11, 35, 171, 637, 994
BceAI	ACGGC	3 cut(s)	108, 733, 1968
BciT130I	CCWGG	3 cut(s)	1320, 1941, 2127
BcnI	CCSGG	3 cut(s)	323, 335, 640
BfaI	CTAG	2 cut(s)	1566, 1868
BfoI	RGCGCY	2 cut(s)	154, 1169
BfrI	CTTAAG	1 cut(s)	953
BfuAI	ACCTGC	1 cut(s)	1877
BlpI	GCTNAGC	1 cut(s)	10
BmcAI	AGTACT	1 cut(s)	1574
Bme1390I	CCNGG	6 cut(s)	323, 335, 640, 1320, 1941, 2127
Bme18I	GGWCC	3 cut(s)	280, 304, 1848
BmeT110I	CYCGRG	2 cut(s)	405, 570
BmgT120I	GGNCC	6 cut(s)	280, 304, 607, 636, 1145, 1848
BmiI	GGNNCC	4 cut(s)	281, 305, 359, 1167
BmrFI	CCNGG	6 cut(s)	323, 335, 640, 1320, 1941, 2127
BmsI	GCATC	3 cut(s)	1011, 1435, 2049
BplI	GAGNNNNNCTC	4 cut(s)	551, 583, 1099, 1131
BpmI	CTGGAG	3 cut(s)	157, 1302, 1441
Bpu1102I	GCTNAGC	1 cut(s)	10
BpuEI	CTTGAG	3 cut(s)	743, 1597, 2036
BpuMI	CCSGG	3 cut(s)	323, 335, 640
BsaHI	GRCGYC	1 cut(s)	1166
BsaI	GGTCTC	5 cut(s)	234, 258, 324, 357, 363
BsaJI	CCNNGG	6 cut(s)	399, 905, 1319, 1653, 1940, 2150
BsaXI	ACNNNNNCTCC	2 cut(s)	296, 326
Bsc4I	CCNNNNNNNGG	7 cut(s)	289, 321, 322, 354, 379, 604, 1552
Bse1I	ACTGG	6 cut(s)	174, 1060, 1366, 1984, 2002, 2100
Bse21I	CCTNAGG	1 cut(s)	735
Bse3DI	GCAATG	2 cut(s)	1044, 1455
BseBI	CCWGG	3 cut(s)	1320, 1941, 2127
BseDI	CCNNGG	6 cut(s)	399, 905, 1319, 1653, 1940, 2150
BseGI	GGATG	6 cut(s)	197, 648, 1026, 1204, 1407, 1489
BseLI	CCNNNNNNNGG	7 cut(s)	289, 321, 322, 354, 379, 604, 1552
BseMI	GCAATG	2 cut(s)	1044, 1455
BseMII	CTCAG	7 cut(s)	24, 726, 834, 918, 1041, 1464, 1649
BseNI	ACTGG	6 cut(s)	174, 1060, 1366, 1984, 2002, 2100
BseSI	GKGCMC	1 cut(s)	418
BseX3I	CGGCCG	1 cut(s)	1137
BseXI	GCAGC	6 cut(s)	48, 193, 678, 733, 757, 1060
BseYI	CCCAGC	3 cut(s)	518, 609, 971
BsgI	GTGCAG	2 cut(s)	192, 2007
Bsh1236I	CGCG	4 cut(s)	102, 190, 441, 1032
Bsh1285I	CGRYCG	2 cut(s)	1140, 1878
BshNI	GGYRCC	1 cut(s)	1165
BsiEI	CGRYCG	2 cut(s)	1140, 1878
BsiHKCI	CYCGRG	2 cut(s)	405, 570
BsiSI	CCGG	3 cut(s)	322, 334, 639
BslFI	GGGAC	4 cut(s)	293, 305, 317, 461
BslI	CCNNNNNNNGG	7 cut(s)	289, 321, 322, 354, 379, 604, 1552
BsmFI	GGGAC	4 cut(s)	293, 305, 317, 461
BsmI	GAATGC	3 cut(s)	586, 1138, 1747
Bso31I	GGTCTC	5 cut(s)	234, 258, 324, 357, 363
BsoBI	CYCGRG	2 cut(s)	405, 570
Bsp1286I	GDGCHC	1 cut(s)	418
Bsp143I	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
Bsp1720I	GCTNAGC	1 cut(s)	10
BspCNI	CTCAG	7 cut(s)	23, 727, 835, 919, 1042, 1465, 1648
BspFNI	CGCG	4 cut(s)	102, 190, 441, 1032
BspHI	TCATGA	2 cut(s)	601, 1647
BspLI	GGNNCC	4 cut(s)	281, 305, 359, 1167
BspMI	ACCTGC	1 cut(s)	1877
BspPI	GGATC	2 cut(s)	493, 1278
BspT107I	GGYRCC	1 cut(s)	1165
BspTI	CTTAAG	1 cut(s)	953
BspTNI	GGTCTC	5 cut(s)	234, 258, 324, 357, 363
BsrBI	CCGCTC	5 cut(s)	289, 352, 385, 445, 1558
BsrDI	GCAATG	2 cut(s)	1044, 1455
BsrI	ACTGG	6 cut(s)	174, 1060, 1366, 1984, 2002, 2100
BssECI	CCNNGG	6 cut(s)	399, 905, 1319, 1653, 1940, 2150
BssMI	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
BssNI	GRCGYC	1 cut(s)	1166
BssSI	CACGAG	1 cut(s)	1173
BssT1I	CCWWGG	3 cut(s)	399, 1653, 2150
Bst2BI	CACGAG	1 cut(s)	1173
Bst2UI	CCWGG	3 cut(s)	1320, 1941, 2127
Bst4CI	ACNGT	2 cut(s)	961, 1956
Bst6I	CTCTTC	2 cut(s)	384, 828
BstACI	GRCGYC	1 cut(s)	1166
BstAFI	CTTAAG	1 cut(s)	953
BstC8I	GCNNGC	8 cut(s)	104, 414, 418, 443, 669, 1182, 1256, 1563
BstDEI	CTNAG	9 cut(s)	10, 421, 735, 843, 927, 1050, 1473, 1635, 1758
BstF5I	GGATG	6 cut(s)	197, 648, 1026, 1204, 1407, 1489
BstFNI	CGCG	4 cut(s)	102, 190, 441, 1032
BstH2I	RGCGCY	2 cut(s)	154, 1169
BstHHI	GCGC	7 cut(s)	153, 192, 441, 751, 1032, 1168, 1219
BstKTI	GATC	7 cut(s)	145, 250, 274, 488, 808, 1273, 2050
BstMBI	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
BstMCI	CGRYCG	2 cut(s)	1140, 1878
BstMWI	GCNNNNNNNGC	3 cut(s)	41, 502, 664
BstNI	CCWGG	3 cut(s)	1320, 1941, 2127
BstSCI	CCNGG	6 cut(s)	321, 333, 638, 1318, 1939, 2125
BstSLI	GKGCMC	1 cut(s)	418
BstUI	CGCG	4 cut(s)	102, 190, 441, 1032
BstV1I	GCAGC	6 cut(s)	48, 193, 678, 733, 757, 1060
BstZI	CGGCCG	1 cut(s)	1137
Bsu36I	CCTNAGG	1 cut(s)	735
BtgZI	GCGATG	2 cut(s)	13, 1047
BtsCI	GGATG	6 cut(s)	197, 648, 1026, 1204, 1407, 1489
BtsI	GCAGTG	1 cut(s)	45
BtsIMutI	CAGTG	4 cut(s)	45, 1373, 1537, 2139
BveI	ACCTGC	1 cut(s)	1877
Cac8I	GCNNGC	8 cut(s)	104, 414, 418, 443, 669, 1182, 1256, 1563
CaiI	CAGNNNCTG	1 cut(s)	2138
CciI	TCATGA	2 cut(s)	601, 1647
CfoI	GCGC	7 cut(s)	153, 192, 441, 751, 1032, 1168, 1219
Cfr13I	GGNCC	6 cut(s)	280, 304, 607, 636, 1145, 1848
CseI	GACGC	1 cut(s)	212
Csp6I	GTAC	7 cut(s)	589, 679, 886, 976, 1573, 1957, 1977
CviAII	CATG	7 cut(s)	602, 796, 1540, 1648, 1799, 2081, 2105
CviQI	GTAC	7 cut(s)	589, 679, 886, 976, 1573, 1957, 1977
DdeI	CTNAG	9 cut(s)	10, 421, 735, 843, 927, 1050, 1473, 1635, 1758
DinI	GGCGCC	1 cut(s)	1167
DpnI	GATC	7 cut(s)	144, 249, 273, 487, 807, 1272, 2049
DpnII	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
EaeI	YGGCCR	3 cut(s)	1137, 1981, 2004
EagI	CGGCCG	1 cut(s)	1137
Eam1104I	CTCTTC	2 cut(s)	384, 828
EarI	CTCTTC	2 cut(s)	384, 828
EciI	GGCGGA	3 cut(s)	31, 370, 449
EclXI	CGGCCG	1 cut(s)	1137
Eco130I	CCWWGG	3 cut(s)	399, 1653, 2150
Eco147I	AGGCCT	2 cut(s)	733, 2111
Eco31I	GGTCTC	5 cut(s)	234, 258, 324, 357, 363
Eco32I	GATATC	1 cut(s)	1781
Eco47I	GGWCC	3 cut(s)	280, 304, 1848
Eco52I	CGGCCG	1 cut(s)	1137
Eco81I	CCTNAGG	1 cut(s)	735
Eco88I	CYCGRG	2 cut(s)	405, 570
EcoO109I	RGGNCCY	1 cut(s)	1145
EcoRII	CCWGG	3 cut(s)	1318, 1939, 2125
EcoRV	GATATC	1 cut(s)	1781
EcoT14I	CCWWGG	3 cut(s)	399, 1653, 2150
EgeI	GGCGCC	1 cut(s)	1167
EheI	GGCGCC	1 cut(s)	1167
ErhI	CCWWGG	3 cut(s)	399, 1653, 2150
FaeI	CATG	7 cut(s)	605, 799, 1543, 1651, 1802, 2084, 2108
FaqI	GGGAC	4 cut(s)	293, 305, 317, 461
FatI	CATG	7 cut(s)	601, 795, 1539, 1647, 1798, 2080, 2104
FauI	CCCGC	5 cut(s)	282, 378, 425, 438, 1247
FokI	GGATG	6 cut(s)	184, 655, 1033, 1211, 1414, 1496
FspBI	CTAG	2 cut(s)	1566, 1868
FspI	TGCGCA	2 cut(s)	750, 1218
GlaI	GCGC	7 cut(s)	152, 191, 440, 750, 1031, 1167, 1218
GsaI	CCCAGC	3 cut(s)	522, 613, 975
GsuI	CTGGAG	3 cut(s)	157, 1302, 1441
HaeII	RGCGCY	2 cut(s)	154, 1169
HapII	CCGG	3 cut(s)	322, 334, 639
HgaI	GACGC	1 cut(s)	212
HhaI	GCGC	7 cut(s)	153, 192, 441, 751, 1032, 1168, 1219
Hin1I	GRCGYC	1 cut(s)	1166
Hin1II	CATG	7 cut(s)	605, 799, 1543, 1651, 1802, 2084, 2108
Hin6I	GCGC	7 cut(s)	151, 190, 439, 749, 1030, 1166, 1217
HinP1I	GCGC	7 cut(s)	151, 190, 439, 749, 1030, 1166, 1217
HincII	GTYRAC	2 cut(s)	1095, 1729
HindII	GTYRAC	2 cut(s)	1095, 1729
HindIII	AAGCTT	2 cut(s)	665, 827
HinfI	GANTC	8 cut(s)	344, 507, 534, 703, 901, 1009, 1582, 1658
HpaI	GTTAAC	1 cut(s)	1729
HpaII	CCGG	3 cut(s)	322, 334, 639
HphI	GGTGA	1 cut(s)	1715
Hpy166II	GTNNAC	3 cut(s)	1095, 1729, 1959
Hpy8I	GTNNAC	3 cut(s)	1095, 1729, 1959
Hpy99I	CGWCG	1 cut(s)	206
HpyAV	CCTTC	6 cut(s)	204, 645, 706, 996, 1136, 1832
HpyCH4III	ACNGT	2 cut(s)	961, 1956
HpyCH4IV	ACGT	1 cut(s)	1752
HpyF10VI	GCNNNNNNNGC	3 cut(s)	41, 502, 664
HpyF3I	CTNAG	9 cut(s)	10, 421, 735, 843, 927, 1050, 1473, 1635, 1758
HpySE526I	ACGT	1 cut(s)	1752
Hsp92I	GRCGYC	1 cut(s)	1166
Hsp92II	CATG	7 cut(s)	605, 799, 1543, 1651, 1802, 2084, 2108
HspAI	GCGC	7 cut(s)	151, 190, 439, 749, 1030, 1166, 1217
KasI	GGCGCC	1 cut(s)	1165
KspAI	GTTAAC	1 cut(s)	1729
Kzo9I	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
LmnI	GCTCC	6 cut(s)	382, 436, 655, 688, 1555, 1816
Lsp1109I	GCAGC	6 cut(s)	48, 193, 678, 733, 757, 1060
LweI	GCATC	3 cut(s)	1011, 1435, 2049
MaeI	CTAG	2 cut(s)	1566, 1868
MaeII	ACGT	1 cut(s)	1752
MaeIII	GTNAC	5 cut(s)	217, 317, 1594, 1786, 1809
MalI	GATC	7 cut(s)	144, 249, 273, 487, 807, 1272, 2049
MbiI	CCGCTC	5 cut(s)	289, 352, 385, 445, 1558
MboI	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
MfeI	CAATTG	1 cut(s)	1599
MhlI	GDGCHC	1 cut(s)	418
MlsI	TGGCCA	1 cut(s)	2006
MluNI	TGGCCA	1 cut(s)	2006
Mly113I	GGCGCC	1 cut(s)	1166
MlyI	GAGTC	1 cut(s)	516
MmeI	TCCRAC	2 cut(s)	142, 225
Mox20I	TGGCCA	1 cut(s)	2006
MroXI	GAANNNNTTC	1 cut(s)	1842
MscI	TGGCCA	1 cut(s)	2006
MseI	TTAA	9 cut(s)	86, 468, 876, 954, 1362, 1628, 1679, 1728, 1926
MslI	CAYNNNNRTG	1 cut(s)	1523
Msp20I	TGGCCA	1 cut(s)	2006
MspA1I	CMGCKG	2 cut(s)	746, 1610
MspCI	CTTAAG	1 cut(s)	953
MspI	CCGG	3 cut(s)	322, 334, 639
MspR9I	CCNGG	6 cut(s)	323, 335, 640, 1320, 1941, 2127
MunI	CAATTG	1 cut(s)	1599
Mva1269I	GAATGC	3 cut(s)	586, 1138, 1747
MvaI	CCWGG	3 cut(s)	1320, 1941, 2127
MvnI	CGCG	4 cut(s)	102, 190, 441, 1032
MwoI	GCNNNNNNNGC	3 cut(s)	41, 502, 664
NarI	GGCGCC	1 cut(s)	1166
NciI	CCSGG	3 cut(s)	323, 335, 640
NdeII	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
NlaIII	CATG	7 cut(s)	605, 799, 1543, 1651, 1802, 2084, 2108
NlaIV	GGNNCC	4 cut(s)	281, 305, 359, 1167
NmuCI	GTSAC	4 cut(s)	317, 1594, 1786, 1809
NsbI	TGCGCA	2 cut(s)	750, 1218
PaeR7I	CTCGAG	1 cut(s)	405
PagI	TCATGA	2 cut(s)	601, 1647
PceI	AGGCCT	2 cut(s)	733, 2111
PcsI	WCGNNNNNNNCGW	2 cut(s)	902, 1972
PctI	GAATGC	3 cut(s)	586, 1138, 1747
PdmI	GAANNNNTTC	1 cut(s)	1842
PfeI	GAWTC	7 cut(s)	344, 534, 703, 901, 1009, 1582, 1658
PleI	GAGTC	1 cut(s)	515
PluTI	GGCGCC	1 cut(s)	1169
PpsI	GAGTC	1 cut(s)	515
PshBI	ATTAAT	1 cut(s)	1926
Psp6I	CCWGG	3 cut(s)	1318, 1939, 2125
PspFI	CCCAGC	3 cut(s)	518, 609, 971
PspGI	CCWGG	3 cut(s)	1318, 1939, 2125
PspN4I	GGNNCC	4 cut(s)	281, 305, 359, 1167
PspPI	GGNCC	6 cut(s)	280, 304, 607, 636, 1145, 1848
PstNI	CAGNNNCTG	1 cut(s)	2138
PvuII	CAGCTG	1 cut(s)	1610
RsaI	GTAC	7 cut(s)	590, 680, 887, 977, 1574, 1958, 1978
RsaNI	GTAC	7 cut(s)	589, 679, 886, 976, 1573, 1957, 1977
RseI	CAYNNNNRTG	1 cut(s)	1523
SaqAI	TTAA	9 cut(s)	86, 468, 876, 954, 1362, 1628, 1679, 1728, 1926
Sau3AI	GATC	7 cut(s)	142, 247, 271, 485, 805, 1270, 2047
Sau96I	GGNCC	6 cut(s)	280, 304, 607, 636, 1145, 1848
ScaI	AGTACT	1 cut(s)	1574
SchI	GAGTC	1 cut(s)	516
ScrFI	CCNGG	6 cut(s)	323, 335, 640, 1320, 1941, 2127
SduI	GDGCHC	1 cut(s)	418
SfaNI	GCATC	3 cut(s)	1011, 1435, 2049
SfoI	GGCGCC	1 cut(s)	1167
Sfr274I	CTCGAG	1 cut(s)	405
SinI	GGWCC	3 cut(s)	280, 304, 1848
SlaI	CTCGAG	1 cut(s)	405
SmiMI	CAYNNNNRTG	1 cut(s)	1523
SmlI	CTYRAG	5 cut(s)	405, 758, 953, 1576, 2051
SmoI	CTYRAG	5 cut(s)	405, 758, 953, 1576, 2051
SseBI	AGGCCT	2 cut(s)	733, 2111
SspDI	GGCGCC	1 cut(s)	1165
SspMI	CTAG	2 cut(s)	1566, 1868
StuI	AGGCCT	2 cut(s)	733, 2111
StyD4I	CCNGG	6 cut(s)	321, 333, 638, 1318, 1939, 2125
StyI	CCWWGG	3 cut(s)	399, 1653, 2150
TaaI	ACNGT	2 cut(s)	961, 1956
TaiI	ACGT	1 cut(s)	1755
TaqI	TCGA	8 cut(s)	250, 406, 488, 543, 896, 1106, 1266, 1950
TaqII	GACCGA	8 cut(s)	252, 258, 282, 297, 321, 381, 387, 1892
TatI	WGTACW	2 cut(s)	1572, 1956
TauI	GCSGC	7 cut(s)	35, 67, 355, 746, 1007, 1139, 1561
TfiI	GAWTC	7 cut(s)	344, 534, 703, 901, 1009, 1582, 1658
Tru1I	TTAA	9 cut(s)	86, 468, 876, 954, 1362, 1628, 1679, 1728, 1926
Tru9I	TTAA	9 cut(s)	86, 468, 876, 954, 1362, 1628, 1679, 1728, 1926
TscAI	CASTG	4 cut(s)	52, 1373, 1537, 2139
TseFI	GTSAC	4 cut(s)	317, 1594, 1786, 1809
TseI	GCWGC	6 cut(s)	61, 206, 691, 746, 770, 1073
Tsp45I	GTSAC	4 cut(s)	317, 1594, 1786, 1809
TspDTI	ATGAA	4 cut(s)	17, 186, 593, 1500
TspGWI	ACGGA	1 cut(s)	572
TspRI	CASTG	4 cut(s)	52, 1373, 1537, 2139
Vha464I	CTTAAG	1 cut(s)	953
VpaK11BI	GGWCC	3 cut(s)	280, 304, 1848
VspI	ATTAAT	1 cut(s)	1926
XapI	RAATTY	1 cut(s)	527
XcmI	CCANNNNNNNNNTGG	2 cut(s)	617, 918
XhoI	CTCGAG	1 cut(s)	405
XmnI	GAANNNNTTC	1 cut(s)	1842
XspI	CTAG	2 cut(s)	1566, 1868
ZrmI	AGTACT	1 cut(s)	1574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.