Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003529

Physical Location & Seq

Genomic Coordinates Forward (+)

18668713 .. 18671037

Gene Structure 2325 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1087300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1170 bp

ATGGCAATGAATCACGACCAACACCAAAACCCACAAAGCCATACACTTTTGCTGGATGCTTTGCTCTGTGAGGAGGACAAGTGGGAGGAGGAGGACGATGGAGAGGTGGTGGAACAAGAAGTGAGTAGTGGTGAGAACTACAACAATGGCGTCAGTGGGAAAAACCCAAACTGCCCTTCTCTGTTTCCCATTCTCCTGTTGGAACAAGATCTGTTCTGGGAGGATGATGAGCTTGTTTCTCTCTTTTCCAAGGAGGAGCAGGAACAGACGCATTTCGTCAAACACATGGAGGGCATGGGAACAGAGGACTCTTTGATCTCCGCGGCTCGCCGTGATGCCGTGCTGTGGATGCTCAAAGTGAACGCCCATCACGGGTTCACAGCCCTCACTGCAATCCTAGCCGTTAACTACTTCGATCGGTTCATCTCAAGCTTCCATTTCCAGAGAGACAAACCGTGGATGGTACAGCTTGTGGCCGTCACTTGCCTCTCTCTGGCTGCCAAAGTGGAAGAAACAGAAGTTCCTCTGCTTTTAGACCTTCAAGTGGAGGACACGAAGTACTTTTTCGAGGCGAAAACGATTCAGAGAATGGAGATTTTGGTGCTCTCAGCTCTCCAGTGGCGGATGCATCCAGTCACACCACTTTCGTTCCTCGACCACATCATAAGAAGGCTCGGATTGAAAACCCACCTCCATTGGGAGTTCCTCAAGCGCTGCGAGCGTCTCCTTCTCTCGGTAGTCTTCGATTCGAGATTTGTCCGTTATTTGCCTTCTGTGTTGGCCACTGCAACAATGATGCGCGTTATAGACCAGGTCGAGCCTTGGAATGCCATTGAATACCAGAACCAGCTTCTTGGTGTTCTCAAAATAAGCAAGGAAAAAGTGAACCACTGTTACAGTCTGATACTCGACTATGATTACGCCTGCTGCAACAACAAAAACAGCACCAACAAACGCAAGCATGATCAAATCCCGGGCAGCCCAAGTGGGGTGATTGATGCATATTTCAGCTATGAAAGCTCAAATGATTCGTGGCCAGTGGGATCATCAGTTTCTTCATCCCCAGAAGCACTTTTCAAGAGGGCCAGAAAAGCAGAAGCAGAAGAGAAAGAGACGAAATTGGCCTCACTCAGCAGGCTCTTTGTGGGCACTGTTGGTAGCCCTCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000082 GO:0000278 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007049 GO:0007275 GO:0008150 GO:0009719 GO:0009725 GO:0009735 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009987 GO:0010033 GO:0010154 GO:0010374 GO:0010440 GO:0010444 GO:0022402 GO:0022414 GO:0030154 GO:0032501 GO:0032502 GO:0034285 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0061458 GO:0090558 GO:0090627 GO:1901700 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Cyclin_C Cyclin_N

Protein Analysis

390

Amino Acids

44.51

Weight (kDa)

5.24

Isoelectric Point (pI)

49.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyclin_N	PF00134	85 - 209	2.9e-27	Cyclin, N-terminal domain
Cyclin_C	PF02984	212 - 319	9.8e-17	Cyclin, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003529)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34160
fragaria_vesca	FvH4_2g05460 FvH4_2g33760 FvH4_2g33760
malus_domestica	MD05G1087300.v1.1 MD08G1092900.v1.1 MD10G1095900.v1.1 MD15G1077100.v1.1
prunus_persica	Prupe.1G430500_v2.0.a1 Prupe.1G430500_v2.0.a1 Prupe.8G131900_v2.0.a1
pyrus_communis	pycom05g08680 pycom08g07550 pycom15g07180
rosa_chinensis	RchiOBHm_Chr6g0255741 RchiOBHm_Chr6g0308791
rosa_laevigata	RLG00000010618 RLG00000014817 RLG00000030143
rosa_multiflora	Rmu_co8197358.1_g000001 Rmu_sc0004046.1_g000004
rosa_roxburghii	Rroxscaffold_7G00159920 Rroxscaffold_7G00209710
rosa_rugosa	Rorug05G0560900 Rorug06G0368300
rosa_samantha	Rh6AG079000 Rh6AG479500 Rh6BG072100 Rh6BG489000 Rh6CG070200 Rh6CG494500 Rh6DG067400 Rh6DG479800
rosa_wichuraiana	Rw6G006810 Rw6G041800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	323, 801
AciI	CCGC	3 cut(s)	321, 323, 622
AclWI	GGATC	1 cut(s)	1051
AcoI	YGGCCR	3 cut(s)	474, 780, 1034
AcyI	GRCGYC	1 cut(s)	150
AfaI	GTAC	2 cut(s)	465, 560
AfeI	AGCGCT	1 cut(s)	713
AfiI	CCNNNNNNNGG	7 cut(s)	345, 372, 493, 544, 697, 733, 988
AgsI	TTSAA	4 cut(s)	542, 682, 836, 1078
AjnI	CCWGG	1 cut(s)	810
AjuI	GAANNNNNNNTTGG	2 cut(s)	160, 192
AluBI	AGCT	7 cut(s)	232, 432, 469, 611, 850, 1011, 1020
AluI	AGCT	7 cut(s)	232, 432, 469, 611, 850, 1011, 1020
Alw21I	GWGCWC	1 cut(s)	606
Alw26I	GTCTC	3 cut(s)	441, 728, 1106
AlwI	GGATC	1 cut(s)	1051
Ama87I	CYCGRG	1 cut(s)	973
Aor51HI	AGCGCT	1 cut(s)	713
AoxI	GGCC	5 cut(s)	474, 780, 1034, 1083, 1122
ApeKI	GCWGC	4 cut(s)	497, 714, 927, 978
AspLEI	GCGC	2 cut(s)	714, 801
AspS9I	GGNCC	1 cut(s)	1083
AsuC2I	CCSGG	2 cut(s)	974, 975
AsuHPI	GGTGA	2 cut(s)	143, 1003
AvaI	CYCGRG	1 cut(s)	973
BaeGI	GKGCMC	1 cut(s)	1151
BalI	TGGCCA	2 cut(s)	782, 1036
BbsI	GAAGAC	1 cut(s)	733
Bbv12I	GWGCWC	1 cut(s)	606
BbvI	GCAGC	4 cut(s)	484, 701, 914, 990
BccI	CCATC	3 cut(s)	92, 375, 454
BceAI	ACGGC	4 cut(s)	315, 323, 386, 461
BciT130I	CCWGG	1 cut(s)	812
BclI	TGATCA	1 cut(s)	964
BcnI	CCSGG	2 cut(s)	974, 975
BcoDI	GTCTC	3 cut(s)	441, 728, 1106
BfaI	CTAG	1 cut(s)	398
BfoI	RGCGCY	1 cut(s)	715
BglII	AGATCT	1 cut(s)	208
BisI	GCNGC	5 cut(s)	324, 498, 715, 928, 979
BlsI	GCNGC	5 cut(s)	325, 499, 716, 929, 980
BmcAI	AGTACT	1 cut(s)	560
Bme1390I	CCNGG	3 cut(s)	812, 974, 975
BmeT110I	CYCGRG	1 cut(s)	973
BmgT120I	GGNCC	1 cut(s)	1083
BmrFI	CCNGG	3 cut(s)	812, 974, 975
BmsI	GCATC	7 cut(s)	46, 325, 339, 615, 637, 786, 988
BpiI	GAAGAC	1 cut(s)	733
BpmI	CTGGAG	1 cut(s)	599
BpuEI	CTTGAG	2 cut(s)	412, 692
BpuMI	CCSGG	2 cut(s)	974, 975
BsaHI	GRCGYC	1 cut(s)	150
BsaJI	CCNNGG	5 cut(s)	249, 321, 455, 821, 973
BsaXI	ACNNNNNCTCC	4 cut(s)	65, 93, 95, 123
Bsc4I	CCNNNNNNNGG	7 cut(s)	345, 372, 493, 544, 697, 733, 988
Bse1I	ACTGG	3 cut(s)	616, 632, 1037
Bse3DI	GCAATG	1 cut(s)	12
BseBI	CCWGG	1 cut(s)	812
BseDI	CCNNGG	5 cut(s)	249, 321, 455, 821, 973
BseGI	GGATG	7 cut(s)	61, 229, 354, 465, 628, 630, 1058
BseLI	CCNNNNNNNGG	7 cut(s)	345, 372, 493, 544, 697, 733, 988
BseMI	GCAATG	1 cut(s)	12
BseMII	CTCAG	2 cut(s)	621, 1144
BseNI	ACTGG	3 cut(s)	616, 632, 1037
BseRI	GAGGAG	4 cut(s)	86, 101, 104, 269
BseSI	GKGCMC	1 cut(s)	1151
BseXI	GCAGC	4 cut(s)	484, 701, 914, 990
Bsh1236I	CGCG	2 cut(s)	323, 801
Bsh1285I	CGRYCG	1 cut(s)	418
BshFI	GGCC	5 cut(s)	476, 782, 1036, 1085, 1124
BsiEI	CGRYCG	1 cut(s)	418
BsiHKAI	GWGCWC	1 cut(s)	606
BsiHKCI	CYCGRG	1 cut(s)	973
BsiSI	CCGG	1 cut(s)	974
BslI	CCNNNNNNNGG	7 cut(s)	345, 372, 493, 544, 697, 733, 988
BsmAI	GTCTC	3 cut(s)	441, 728, 1106
BsmBI	CGTCTC	2 cut(s)	728, 1106
BsmI	GAATGC	1 cut(s)	832
BsnI	GGCC	5 cut(s)	476, 782, 1036, 1085, 1124
BsoBI	CYCGRG	1 cut(s)	973
Bsp1286I	GDGCHC	2 cut(s)	606, 1151
Bsp143I	GATC	5 cut(s)	208, 315, 415, 964, 1043
BspACI	CCGC	3 cut(s)	321, 323, 622
BspANI	GGCC	5 cut(s)	476, 782, 1036, 1085, 1124
BspCNI	CTCAG	2 cut(s)	620, 1143
BspFNI	CGCG	2 cut(s)	323, 801
BspPI	GGATC	1 cut(s)	1051
BsrDI	GCAATG	1 cut(s)	12
BsrI	ACTGG	3 cut(s)	616, 632, 1037
BssECI	CCNNGG	5 cut(s)	249, 321, 455, 821, 973
BssMI	GATC	5 cut(s)	208, 315, 415, 964, 1043
BssNI	GRCGYC	1 cut(s)	150
BssT1I	CCWWGG	2 cut(s)	249, 821
Bst2UI	CCWGG	1 cut(s)	812
Bst4CI	ACNGT	4 cut(s)	456, 893, 899, 1153
Bst6I	CTCTTC	1 cut(s)	1098
BstACI	GRCGYC	1 cut(s)	150
BstC8I	GCNNGC	5 cut(s)	328, 719, 925, 959, 1136
BstDEI	CTNAG	2 cut(s)	607, 1130
BstDSI	CCRYGG	2 cut(s)	321, 455
BstF5I	GGATG	7 cut(s)	61, 229, 354, 465, 628, 630, 1058
BstFNI	CGCG	2 cut(s)	323, 801
BstH2I	RGCGCY	1 cut(s)	715
BstHHI	GCGC	2 cut(s)	714, 801
BstKTI	GATC	5 cut(s)	211, 318, 418, 967, 1046
BstMAI	GTCTC	3 cut(s)	441, 728, 1106
BstMBI	GATC	5 cut(s)	208, 315, 415, 964, 1043
BstMCI	CGRYCG	1 cut(s)	418
BstMWI	GCNNNNNNNGC	6 cut(s)	349, 389, 398, 718, 1017, 1091
BstNI	CCWGG	1 cut(s)	812
BstSCI	CCNGG	3 cut(s)	810, 972, 973
BstSLI	GKGCMC	1 cut(s)	1151
BstUI	CGCG	2 cut(s)	323, 801
BstV1I	GCAGC	4 cut(s)	484, 701, 914, 990
BstV2I	GAAGAC	1 cut(s)	733
BstX2I	RGATCY	1 cut(s)	208
BstXI	CCANNNNNNTGG	1 cut(s)	854
BstYI	RGATCY	1 cut(s)	208
BsuRI	GGCC	5 cut(s)	476, 782, 1036, 1085, 1124
BtgI	CCRYGG	2 cut(s)	321, 455
BtsCI	GGATG	7 cut(s)	61, 229, 354, 465, 628, 630, 1058
BtsI	GCAGTG	2 cut(s)	387, 783
BtsIMutI	CAGTG	7 cut(s)	160, 387, 623, 783, 889, 1044, 1149
Cac8I	GCNNGC	5 cut(s)	328, 719, 925, 959, 1136
CfoI	GCGC	2 cut(s)	714, 801
Cfr13I	GGNCC	1 cut(s)	1083
Cfr42I	CCGCGG	1 cut(s)	324
Cfr9I	CCCGGG	1 cut(s)	973
CseI	GACGC	3 cut(s)	139, 277, 710
CsiI	ACCWGGT	1 cut(s)	810
Csp6I	GTAC	2 cut(s)	464, 559
CspCI	CAANNNNNGTGG	2 cut(s)	677, 712
CviAII	CATG	3 cut(s)	286, 295, 962
CviQI	GTAC	2 cut(s)	464, 559
DdeI	CTNAG	2 cut(s)	607, 1130
DpnI	GATC	5 cut(s)	210, 317, 417, 966, 1045
DpnII	GATC	5 cut(s)	208, 315, 415, 964, 1043
EaeI	YGGCCR	3 cut(s)	474, 780, 1034
Eam1104I	CTCTTC	1 cut(s)	1098
EarI	CTCTTC	1 cut(s)	1098
EciI	GGCGGA	1 cut(s)	637
Eco130I	CCWWGG	2 cut(s)	249, 821
Eco47III	AGCGCT	1 cut(s)	713
Eco88I	CYCGRG	1 cut(s)	973
EcoRII	CCWGG	1 cut(s)	810
EcoT14I	CCWWGG	2 cut(s)	249, 821
EcoT22I	ATGCAT	2 cut(s)	630, 1003
ErhI	CCWWGG	2 cut(s)	249, 821
Esp3I	CGTCTC	2 cut(s)	728, 1106
FaeI	CATG	3 cut(s)	289, 298, 965
FaiI	YATR	9 cut(s)	42, 287, 296, 665, 806, 915, 963, 1003, 1014
FatI	CATG	3 cut(s)	285, 294, 961
FbaI	TGATCA	1 cut(s)	964
Fnu4HI	GCNGC	5 cut(s)	324, 498, 715, 928, 979
FokI	GGATG	7 cut(s)	68, 236, 361, 472, 615, 637, 1045
Fsp4HI	GCNGC	5 cut(s)	324, 498, 715, 928, 979
FspBI	CTAG	1 cut(s)	398
GlaI	GCGC	2 cut(s)	713, 800
GluI	GCNGC	5 cut(s)	324, 498, 715, 928, 979
GsuI	CTGGAG	1 cut(s)	599
HaeII	RGCGCY	1 cut(s)	715
HaeIII	GGCC	5 cut(s)	476, 782, 1036, 1085, 1124
HapII	CCGG	1 cut(s)	974
HgaI	GACGC	3 cut(s)	139, 277, 710
HhaI	GCGC	2 cut(s)	714, 801
Hin1I	GRCGYC	1 cut(s)	150
Hin1II	CATG	3 cut(s)	289, 298, 965
Hin6I	GCGC	2 cut(s)	712, 799
HinP1I	GCGC	2 cut(s)	712, 799
HincII	GTYRAC	1 cut(s)	406
HindII	GTYRAC	1 cut(s)	406
HindIII	AAGCTT	1 cut(s)	430
HinfI	GANTC	5 cut(s)	10, 308, 580, 746, 1028
HpaI	GTTAAC	1 cut(s)	406
HpaII	CCGG	1 cut(s)	974
HphI	GGTGA	2 cut(s)	143, 1003
Hpy166II	GTNNAC	4 cut(s)	361, 378, 406, 886
Hpy188I	TCNGA	3 cut(s)	585, 677, 903
Hpy188III	TCNNGA	4 cut(s)	14, 442, 750, 1078
Hpy8I	GTNNAC	4 cut(s)	361, 378, 406, 886
HpyAV	CCTTC	5 cut(s)	186, 548, 663, 737, 780
HpyCH4III	ACNGT	4 cut(s)	456, 893, 899, 1153
HpyCH4V	TGCA	5 cut(s)	392, 628, 788, 930, 1001
HpyF10VI	GCNNNNNNNGC	6 cut(s)	349, 389, 398, 718, 1017, 1091
HpyF3I	CTNAG	2 cut(s)	607, 1130
Hsp92I	GRCGYC	1 cut(s)	150
Hsp92II	CATG	3 cut(s)	289, 298, 965
HspAI	GCGC	2 cut(s)	712, 799
Ksp22I	TGATCA	1 cut(s)	964
KspAI	GTTAAC	1 cut(s)	406
KspI	CCGCGG	1 cut(s)	324
Kzo9I	GATC	5 cut(s)	208, 315, 415, 964, 1043
LmnI	GCTCC	1 cut(s)	256
Lsp1109I	GCAGC	4 cut(s)	484, 701, 914, 990
LweI	GCATC	7 cut(s)	46, 325, 339, 615, 637, 786, 988
MabI	ACCWGGT	1 cut(s)	810
MaeI	CTAG	1 cut(s)	398
MaeIII	GTNAC	3 cut(s)	478, 634, 893
MalI	GATC	5 cut(s)	210, 317, 417, 966, 1045
MboI	GATC	5 cut(s)	208, 315, 415, 964, 1043
MboII	GAAGA	4 cut(s)	521, 733, 1047, 1115
MflI	RGATCY	1 cut(s)	208
MhlI	GDGCHC	2 cut(s)	606, 1151
MlsI	TGGCCA	2 cut(s)	782, 1036
MluCI	AATT	1 cut(s)	1118
MluNI	TGGCCA	2 cut(s)	782, 1036
MlyI	GAGTC	1 cut(s)	302
MmeI	TCCRAC	1 cut(s)	180
Mox20I	TGGCCA	2 cut(s)	782, 1036
Mph1103I	ATGCAT	2 cut(s)	630, 1003
MscI	TGGCCA	2 cut(s)	782, 1036
MseI	TTAA	2 cut(s)	405, 1168
Msp20I	TGGCCA	2 cut(s)	782, 1036
MspA1I	CMGCKG	1 cut(s)	323
MspI	CCGG	1 cut(s)	974
MspR9I	CCNGG	3 cut(s)	812, 974, 975
Mva1269I	GAATGC	1 cut(s)	832
MvaI	CCWGG	1 cut(s)	812
MvnI	CGCG	2 cut(s)	323, 801
MwoI	GCNNNNNNNGC	6 cut(s)	349, 389, 398, 718, 1017, 1091
NciI	CCSGG	2 cut(s)	974, 975
NdeII	GATC	5 cut(s)	208, 315, 415, 964, 1043
NlaIII	CATG	3 cut(s)	289, 298, 965
NmuCI	GTSAC	2 cut(s)	478, 634
NsiI	ATGCAT	2 cut(s)	630, 1003
PctI	GAATGC	1 cut(s)	832
PfeI	GAWTC	4 cut(s)	10, 580, 746, 1028
PflFI	GACNNNGTC	1 cut(s)	812
PkrI	GCNGC	5 cut(s)	325, 499, 716, 929, 980
Ple19I	CGATCG	1 cut(s)	418
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
Psp6I	CCWGG	1 cut(s)	810
PspGI	CCWGG	1 cut(s)	810
PspPI	GGNCC	1 cut(s)	1083
PsrI	GAACNNNNNNTAC	2 cut(s)	878, 910
PsuI	RGATCY	1 cut(s)	208
PsyI	GACNNNGTC	1 cut(s)	812
PvuI	CGATCG	1 cut(s)	418
RsaI	GTAC	2 cut(s)	465, 560
RsaNI	GTAC	2 cut(s)	464, 559
SacII	CCGCGG	1 cut(s)	324
SaqAI	TTAA	2 cut(s)	405, 1168
SatI	GCNGC	5 cut(s)	324, 498, 715, 928, 979
Sau3AI	GATC	5 cut(s)	208, 315, 415, 964, 1043
Sau96I	GGNCC	1 cut(s)	1083
ScaI	AGTACT	1 cut(s)	560
SchI	GAGTC	1 cut(s)	302
ScrFI	CCNGG	3 cut(s)	812, 974, 975
SduI	GDGCHC	2 cut(s)	606, 1151
SexAI	ACCWGGT	1 cut(s)	810
SfaNI	GCATC	7 cut(s)	46, 325, 339, 615, 637, 786, 988
Sfr303I	CCGCGG	1 cut(s)	324
SgrBI	CCGCGG	1 cut(s)	324
SmaI	CCCGGG	1 cut(s)	975
SmlI	CTYRAG	2 cut(s)	427, 707
SmoI	CTYRAG	2 cut(s)	427, 707
Sse9I	AATT	1 cut(s)	1118
SsiI	CCGC	3 cut(s)	321, 323, 622
SspMI	CTAG	1 cut(s)	398
StyD4I	CCNGG	3 cut(s)	810, 972, 973
StyI	CCWWGG	2 cut(s)	249, 821
TaaI	ACNGT	4 cut(s)	456, 893, 899, 1153
TaqI	TCGA	7 cut(s)	414, 567, 654, 744, 749, 816, 909
TasI	AATT	1 cut(s)	1118
TatI	WGTACW	1 cut(s)	558
TauI	GCSGC	1 cut(s)	326
TfiI	GAWTC	4 cut(s)	10, 580, 746, 1028
Tru1I	TTAA	2 cut(s)	405, 1168
Tru9I	TTAA	2 cut(s)	405, 1168
TscAI	CASTG	7 cut(s)	160, 394, 623, 790, 896, 1044, 1156
TseFI	GTSAC	2 cut(s)	478, 634
TseI	GCWGC	4 cut(s)	497, 714, 927, 978
Tsp45I	GTSAC	2 cut(s)	478, 634
TspDTI	ATGAA	4 cut(s)	23, 412, 1029, 1047
TspGWI	ACGGA	1 cut(s)	749
TspMI	CCCGGG	1 cut(s)	973
TspRI	CASTG	7 cut(s)	160, 394, 623, 790, 896, 1044, 1156
Tth111I	GACNNNGTC	1 cut(s)	812
XcmI	CCANNNNNNNNNTGG	1 cut(s)	196
XmaI	CCCGGG	1 cut(s)	973
XspI	CTAG	1 cut(s)	398
ZrmI	AGTACT	1 cut(s)	560
Zsp2I	ATGCAT	2 cut(s)	630, 1003

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.