MD05G1214600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004226
Physical Location & Seq
Forward (+)
34569263 .. 34569670
408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1214600.v1.1.491

Sequence Viewer

Length: 408 bp
ATGTTAACTGCGGGATGCCCAGACACGGTTTGGAGGCATTTCGCGAATGGAGTGAGACCCAATATAGTAACAGTTTCTTCAATCCTACCTGCTTGCTCGGAGTTGAAAGATTTGAATTTGGAAAGAGTGATTCATGGGTTTCTGGTCAGACATGGAATGGAAGAGAATGTTTTGGTTAGCAGCGCCCTTGTAAATATCTATGCTAGTTGTCTGAGCACTAAACAAGCTCAGTTGGTATTTGATATGATGTCTAGACGAGATGTCGTGTCCTGGAATGTACATCTAACTGCGTATTTTTCGAACAGAGATTGCAAAAAGGGTGTAACTTTATTTTCTAGGATGAGAAGGGAAGGTGTTAAATTGGATGGTGCTTCATGGAATGCTGTTATTGGTGGGGTGCTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

136

Amino Acids

15.02

Weight (kDa)

8.82

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 86 - 132 1.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 97
AccII CGCG 1 cut(s) 44
AciI CCGC 1 cut(s) 11
AcsI RAATTY 1 cut(s) 115
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 4 cut(s) 81, 106, 115, 404
AhdI GACNNNNNGTC 1 cut(s) 260
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 1 cut(s) 227
AluI AGCT 1 cut(s) 227
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 1 cut(s) 115
AspLEI GCGC 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 192
BccI CCATC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 3 cut(s) 204, 252, 336
BfoI RGCGCY 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 271
BmeRI GACNNNNNGTC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 5
Bpu14I TTCGAA 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 25
BseBI CCWGG 1 cut(s) 271
BseGI GGATG 3 cut(s) 20, 345, 370
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 203, 242
BseXI GCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 385
Bso31I GGTCTC 1 cut(s) 49
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 218
Bsp1407I TGTACA 1 cut(s) 277
Bsp68I TCGCGA 1 cut(s) 44
BspACI CCGC 1 cut(s) 11
BspCNI CTCAG 2 cut(s) 204, 241
BspFNI CGCG 1 cut(s) 44
BspMI ACCTGC 1 cut(s) 97
BspT104I TTCGAA 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 2 cut(s) 28, 73
Bst6I CTCTTC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 277
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 2 cut(s) 212, 228
BstF5I GGATG 3 cut(s) 20, 345, 370
BstFNI CGCG 1 cut(s) 44
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstMAI GTCTC 1 cut(s) 49
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 269
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 192
BtsCI GGATG 3 cut(s) 20, 345, 370
BtuMI TCGCGA 1 cut(s) 44
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 94
CfoI GCGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 278
CviAII CATG 3 cut(s) 134, 152, 375
CviJI RGCY 1 cut(s) 227
CviKI_1 RGCY 1 cut(s) 227
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 2 cut(s) 212, 228
DriI GACNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 156
Eam1105I GACNNNNNGTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 269
FaeI CATG 3 cut(s) 137, 155, 378
FaiI YATR 6 cut(s) 65, 135, 153, 201, 245, 376
FatI CATG 3 cut(s) 133, 151, 374
FauI CCCGC 1 cut(s) 4
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 3 cut(s) 27, 352, 377
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 3 cut(s) 204, 252, 336
GlaI GCGC 1 cut(s) 184
GluI GCNGC 1 cut(s) 181
HaeII RGCGCY 1 cut(s) 186
HhaI GCGC 1 cut(s) 185
Hin1II CATG 3 cut(s) 137, 155, 378
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 130
HpaI GTTAAC 1 cut(s) 6
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 100, 149, 213
Hpy188III TCNNGA 2 cut(s) 43, 252
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 2 cut(s) 339, 344
HpyCH4III ACNGT 2 cut(s) 28, 73
HpyCH4V TGCA 1 cut(s) 312
HpyF3I CTNAG 2 cut(s) 212, 228
Hsp92II CATG 3 cut(s) 137, 155, 378
HspAI GCGC 1 cut(s) 183
KspAI GTTAAC 1 cut(s) 6
LpnPI CCDG 5 cut(s) 33, 102, 128, 256, 283
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 1 cut(s) 5
MaeI CTAG 3 cut(s) 204, 252, 336
MaeIII GTNAC 2 cut(s) 67, 322
MboII GAAGA 2 cut(s) 69, 173
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 2 cut(s) 115, 359
MnlI CCTC 1 cut(s) 27
MseI TTAA 2 cut(s) 5, 357
MspR9I CCNGG 1 cut(s) 271
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 1 cut(s) 271
MvnI CGCG 1 cut(s) 44
NlaIII CATG 3 cut(s) 137, 155, 378
NruI TCGCGA 1 cut(s) 44
NspV TTCGAA 1 cut(s) 299
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 130
PfoI TCCNGGA 1 cut(s) 269
PkrI GCNGC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 269
RruI TCGCGA 1 cut(s) 44
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 5, 357
SatI GCNGC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 1 cut(s) 218
SetI ASST 3 cut(s) 91, 229, 355
SfaNI GCATC 1 cut(s) 5
SfuI TTCGAA 1 cut(s) 299
Sse9I AATT 2 cut(s) 115, 359
SsiI CCGC 1 cut(s) 11
SspMI CTAG 3 cut(s) 204, 252, 336
StyD4I CCNGG 1 cut(s) 269
TaaI ACNGT 2 cut(s) 28, 73
TaqI TCGA 1 cut(s) 299
TasI AATT 2 cut(s) 115, 359
TatI WGTACW 1 cut(s) 277
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 2 cut(s) 5, 357
Tru9I TTAA 2 cut(s) 5, 357
TseI GCWGC 1 cut(s) 180
TspDTI ATGAA 2 cut(s) 122, 363
XapI RAATTY 1 cut(s) 115
XbaI TCTAGA 1 cut(s) 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 27
XspI CTAG 3 cut(s) 204, 252, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.