MD10G1199000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004226
Physical Location & Seq
Reverse (-)
29683101 .. 29685161
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1199000.v1.1.491

Sequence Viewer

Length: 2061 bp
ATGCTTTCAAAACTACCCACCAACGTCCCGCCTCACCTGAGCCTTAGGTTCTTGAAAATCTACTGTAATTCCGGCGATCTACAACGTGCACGCCACCTGTTCGATCAAATTCCCGAACCAGACCTACATGCGTGGACCGTGCTCGTATCAGGCTACACGCGACATGGGTTCCTCAAGGAATCTATGAGTCTTTACGCTTCACTCAGAGCTCGGCGAATAGCGCCCGATAGCCTTCTGCTTTTATCAGTTGCTAAGGCGTGTGCTGCTCTTGGTGATGTTAGAAATGCTAAAGAGCTTCACGATGAAACAATTCGATTTGGGTTTCATTCGGATGTCGCTTTGGGTAATGCAATGGTCGATATGTTTGGAAAATGTAGATATGTTGATGGGGCGAGACGGGTTTTCGACAGCATGGCGGTGAAAGATGTGGTGTCTTGGACCTCTTTGTGTTCTTGTTATGTTAACTGCGGGCTGCCTGGGCAAGGTTTGGCGGCATTTCGAGAGATGGGGTTGAATGGAGTGAGACCCAATGCTGTGACAGTGTCTTCAATCCTACCTGCTTGCTCAGAACTGAAAGATTTGAAATTGGGAAGAGAGATTCATGGGTTTGTTCTCAAACATGGAATGGAAGAGAATGTTTTCGTTAGCAGCGCCCTTGTAAATATCTATGCTAGTTGTCTGAGCATTAAACAAGCTCAGTTGGTATTTGATATGATGTCTAAACGAGATGTCGTATCCTGGAATGTACTTCTAACTGCATATTTCTCGAACAGAGATTGCAAAAAGGGCGTAGCTTTATTTTGTAGGATGAGAAGGGAAGGTGTTAAATTGGATGGTGCTTCATGGAATGCTGTTATTGGTGGGTGCTTGAATAATGGGCAGACAATACAGGCTCTGAAAATGCTCGGACAGATGCAAGAATCAGGAATTAAGCCCAATCAGATCACAATTACTAGTTTGTTACCAGCTTGCAAAGATTTGGAGAGCTTAAGGGCAGGCAAAGAGGTGCATAGTTACATCTTTAGGACTTGTTTAATGGAGGACTTGGCTACCACAACAGCTTTGGTTTTCATGTATGCAAAATGTGGTGAGTTGGAACTTTCTCGGAGGGTCTTTGATATGATGCCAAGAACGGATACAGTTGCTTGGAATACGATGATCATTGCAAACTCGATGCATGGGGATGGAGAAGAAGCCTTGTCGCTGTTCAGGAAAATGTTAGACTCCAAGGTGAAGCCCAATTCTGTTACTTTCACTGGTGTTTTGTGTGGTTGTAGCCATTCTCGGCTGGTTGATGAAGGCATCGTGGTTTTTGATTCAATGAGGACGGATCACTCAATAGAACCTGATGCAGATCATTACTCATGCATGGTTGATGTGCTTAGCCGTGCTGGTCGTCTAGTTGAGGCATATCAGTTCATACAAAGAATGCCAATGGAACCTACTCCCGGTGCTTGGGGAGCATTGCTTGGTGCGTGTAGAGTGCATAAAAATGTGGACTTAGCAAAAATTGCAGCAAACAAGCTGTTCGAAATTGAACCAGATAACCCCGGCAACTATGTACTGTTGTCCAACATTTTAGTCACTGCCAATCGATGGGAAGAAGCTTCGGAAACCAGGAAGTTGATGAGAGACAGAGGAATCACAAAAACACCGGGTTGTTCTTGGGTTCAGGTTAGAAATAGAGTCTACTCTTTTGTTTCCGGTGATAGGAGTAACGAGCGTAGCGAGGAGATATACAAGTTTTTGGTTGACATCGGTGAGAAGATGAGATTGGCAGGGTATGTCCCCAACACTGATTTTGTTCTGCAAGATGTGGATCAAGAGGAGAAAGTGGGGATCCTTTGCAACCATAGTGAGAAGCTTGCTGTAGCTTTTGGGATACTCAATTTGAATGGCGAATCGACTATTCGGGTATTTAAGAATTTGAGGATATGTGGGGACTGCCATAATGCCATCAAGTTCATGGCCAAGATTGTTGGTGTGCAGATTATTGTTAGAGATTCCTTGAGGTTTCACCATTTCAAAAATGGAGATTGTTCTTGTAGAGATTTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

687

Amino Acids

76.61

Weight (kDa)

8.4

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 141 - 189 2e-06 PPR repeat family
PPR_2 PF13041 242 - 278 1.5e-06 PPR repeat family
PPR_3 PF13812 267 - 324 9.7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 280 - 324 5e-10 PPR repeat family
PPR_2 PF13041 379 - 426 1.1e-11 PPR repeat family
PPR PF01535 381 - 410 1.5e-06 PPR repeat
E_motif PF20431 496 - 558 4.3e-22 E motif
DYW_deaminase PF14432 594 - 686 1.9e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB7I CCANNNNNTGG 1 cut(s) 1596
AccI GTMKAC 1 cut(s) 1689
AccII CGCG 1 cut(s) 160
AciI CCGC 4 cut(s) 29, 416, 468, 491
AclWI GGATC 4 cut(s) 1338, 1827, 1834, 1847
AcoI YGGCCR 1 cut(s) 1968
AcsI RAATTY 2 cut(s) 108, 1924
AfaI GTAC 2 cut(s) 747, 1563
AfiI CCNNNNNNNGG 5 cut(s) 482, 1448, 1455, 1596, 1710
AflII CTTAAG 1 cut(s) 988
AhlI ACTAGT 1 cut(s) 953
AjnI CCWGG 3 cut(s) 475, 737, 1616
AjuI GAANNNNNNNTTGG 2 cut(s) 1757, 1789
Alw21I GWGCWC 3 cut(s) 91, 144, 211
Alw26I GTCTC 3 cut(s) 388, 517, 1626
Alw44I GTGCAC 1 cut(s) 87
AlwI GGATC 4 cut(s) 1338, 1827, 1834, 1847
AlwNI CAGNNNCTG 1 cut(s) 895
AoxI GGCC 1 cut(s) 1968
ApaLI GTGCAC 1 cut(s) 87
ApeKI GCWGC 4 cut(s) 263, 472, 648, 1514
ApoI RAATTY 2 cut(s) 108, 1924
Asp700I GAANNNNTTC 2 cut(s) 309, 638
AspLEI GCGC 2 cut(s) 223, 653
AspS9I GGNCC 2 cut(s) 135, 438
AsuC2I CCSGG 3 cut(s) 1449, 1551, 1656
AsuHPI GGTGA 8 cut(s) 26, 284, 430, 1100, 1243, 1718, 1772, 2009
AsuII TTCGAA 1 cut(s) 1530
AvaII GGWCC 2 cut(s) 135, 438
AxyI CCTNAGG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 91
BalI TGGCCA 1 cut(s) 1970
BamHI GGATCC 1 cut(s) 1839
BanII GRGCYC 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 3 cut(s) 91, 144, 211
BbvI GCAGC 4 cut(s) 250, 459, 660, 1526
BccI CCATC 6 cut(s) 380, 499, 827, 1178, 1590, 1964
BceAI ACGGC 1 cut(s) 1371
BcgI CGANNNNNNTGC 2 cut(s) 747, 781
BciT130I CCWGG 3 cut(s) 477, 739, 1618
BciVI GTATCC 3 cut(s) 745, 1129, 1875
BclI TGATCA 1 cut(s) 1158
BcnI CCSGG 3 cut(s) 1449, 1551, 1656
BcoDI GTCTC 3 cut(s) 388, 517, 1626
BcuI ACTAGT 1 cut(s) 953
BfaI CTAG 3 cut(s) 672, 954, 1400
BfmI CTRYAG 1 cut(s) 1869
BfoI RGCGCY 2 cut(s) 224, 654
BfrI CTTAAG 1 cut(s) 988
BfuAI ACCTGC 1 cut(s) 565
BfuI GTATCC 3 cut(s) 745, 1129, 1875
BisI GCNGC 5 cut(s) 264, 473, 492, 649, 1515
BlpI GCTNAGC 1 cut(s) 1382
BlsI GCNGC 5 cut(s) 265, 474, 493, 650, 1516
Bme1390I CCNGG 6 cut(s) 477, 739, 1449, 1551, 1618, 1656
Bme18I GGWCC 2 cut(s) 135, 438
BmgT120I GGNCC 2 cut(s) 135, 438
BmiI GGNNCC 3 cut(s) 170, 1440, 1841
BmrFI CCNGG 6 cut(s) 477, 739, 1449, 1551, 1618, 1656
BmsI GCATC 5 cut(s) 903, 1113, 1164, 1311, 1339
BpiI GAAGAC 1 cut(s) 537
Bpu10I CCTNAGC 2 cut(s) 38, 252
Bpu1102I GCTNAGC 1 cut(s) 1382
Bpu14I TTCGAA 1 cut(s) 1530
BpuEI CTTGAG 2 cut(s) 158, 2029
BpuMI CCSGG 3 cut(s) 1449, 1551, 1656
Bsa29I ATCGAT 1 cut(s) 1594
BsaBI GATNNNNATC 2 cut(s) 1353, 1818
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 3 cut(s) 476, 1227, 1549
BsaWI WCCGGW 1 cut(s) 1703
Bsc4I CCNNNNNNNGG 5 cut(s) 482, 1448, 1455, 1596, 1710
Bse1I ACTGG 1 cut(s) 1261
Bse21I CCTNAGG 1 cut(s) 44
Bse3DI GCAATG 3 cut(s) 357, 1161, 1463
Bse8I GATNNNNATC 2 cut(s) 1353, 1818
BseBI CCWGG 3 cut(s) 477, 739, 1618
BseCI ATCGAT 1 cut(s) 1594
BseDI CCNNGG 3 cut(s) 476, 1227, 1549
BseGI GGATG 4 cut(s) 337, 813, 838, 1189
BseJI GATNNNNATC 2 cut(s) 1353, 1818
BseLI CCNNNNNNNGG 5 cut(s) 482, 1448, 1455, 1596, 1710
BseMI GCAATG 3 cut(s) 357, 1161, 1463
BseMII CTCAG 5 cut(s) 29, 217, 579, 671, 710
BseNI ACTGG 1 cut(s) 1261
BseRI GAGGAG 2 cut(s) 1745, 1841
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 4 cut(s) 250, 459, 660, 1526
BsgI GTGCAG 1 cut(s) 2006
Bsh1236I CGCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 1970
BshVI ATCGAT 1 cut(s) 1594
BsiHKAI GWGCWC 3 cut(s) 91, 144, 211
BsiSI CCGG 5 cut(s) 72, 1449, 1551, 1655, 1704
BslFI GGGAC 3 cut(s) 11, 1772, 1955
BslI CCNNNNNNNGG 5 cut(s) 482, 1448, 1455, 1596, 1710
BsmAI GTCTC 3 cut(s) 388, 517, 1626
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 3 cut(s) 11, 1772, 1955
BsmI GAATGC 2 cut(s) 853, 1434
BsnI GGCC 1 cut(s) 1970
Bso31I GGTCTC 1 cut(s) 517
Bsp119I TTCGAA 1 cut(s) 1530
Bsp1286I GDGCHC 3 cut(s) 91, 144, 211
Bsp143I GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
Bsp1720I GCTNAGC 1 cut(s) 1382
BspACI CCGC 4 cut(s) 29, 416, 468, 491
BspANI GGCC 1 cut(s) 1970
BspCNI CTCAG 5 cut(s) 30, 216, 578, 672, 709
BspDI ATCGAT 1 cut(s) 1594
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 170, 1440, 1841
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 4 cut(s) 1338, 1827, 1834, 1847
BspT104I TTCGAA 1 cut(s) 1530
BspTI CTTAAG 1 cut(s) 988
BspTNI GGTCTC 1 cut(s) 517
BsrDI GCAATG 3 cut(s) 357, 1161, 1463
BsrI ACTGG 1 cut(s) 1261
BssECI CCNNGG 3 cut(s) 476, 1227, 1549
BssMI GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
BssT1I CCWWGG 1 cut(s) 1227
Bst2UI CCWGG 3 cut(s) 477, 739, 1618
Bst4CI ACNGT 5 cut(s) 65, 139, 541, 1141, 1566
Bst6I CTCTTC 2 cut(s) 586, 624
BstAFI CTTAAG 1 cut(s) 988
BstAPI GCANNNNNTGC 1 cut(s) 1511
BstBI TTCGAA 1 cut(s) 1530
BstC8I GCNNGC 6 cut(s) 91, 470, 562, 970, 997, 1866
BstDEI CTNAG 9 cut(s) 38, 44, 203, 252, 565, 680, 696, 1382, 1501
BstENI CCTNNNNNAGG 1 cut(s) 480
BstF5I GGATG 4 cut(s) 337, 813, 838, 1189
BstFNI CGCG 1 cut(s) 160
BstH2I RGCGCY 2 cut(s) 224, 654
BstHHI GCGC 2 cut(s) 223, 653
BstKTI GATC 8 cut(s) 79, 106, 945, 1161, 1333, 1357, 1822, 1842
BstMAI GTCTC 3 cut(s) 388, 517, 1626
BstMBI GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
BstMWI GCNNNNNNNGC 6 cut(s) 220, 263, 478, 786, 1460, 1511
BstNI CCWGG 3 cut(s) 477, 739, 1618
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 6 cut(s) 475, 737, 1447, 1549, 1616, 1654
BstSFI CTRYAG 1 cut(s) 1869
BstSLI GKGCMC 1 cut(s) 91
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 4 cut(s) 250, 459, 660, 1526
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 1839
BstYI RGATCY 1 cut(s) 1839
Bsu15I ATCGAT 1 cut(s) 1594
Bsu36I CCTNAGG 1 cut(s) 44
BsuI GTATCC 3 cut(s) 745, 1129, 1875
BsuRI GGCC 1 cut(s) 1970
BsuTUI ATCGAT 1 cut(s) 1594
BtsCI GGATG 4 cut(s) 337, 813, 838, 1189
BtsI GCAGTG 1 cut(s) 1584
BtsIMutI CAGTG 4 cut(s) 546, 1254, 1584, 1794
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 6 cut(s) 91, 470, 562, 970, 997, 1866
CaiI CAGNNNCTG 1 cut(s) 895
CfoI GCGC 2 cut(s) 223, 653
Cfr13I GGNCC 2 cut(s) 135, 438
ClaI ATCGAT 1 cut(s) 1594
Csp6I GTAC 2 cut(s) 746, 1562
CviQI GTAC 2 cut(s) 746, 1562
DdeI CTNAG 9 cut(s) 38, 44, 203, 252, 565, 680, 696, 1382, 1501
DpnI GATC 8 cut(s) 78, 105, 944, 1160, 1332, 1356, 1821, 1841
DpnII GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
EaeI YGGCCR 1 cut(s) 1968
Eam1104I CTCTTC 2 cut(s) 586, 624
EarI CTCTTC 2 cut(s) 586, 624
Ecl136II GAGCTC 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 1227
Eco24I GRGCYC 1 cut(s) 211
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 2 cut(s) 135, 438
Eco53kI GAGCTC 1 cut(s) 209
Eco81I CCTNAGG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 209
EcoNI CCTNNNNNAGG 1 cut(s) 480
EcoRII CCWGG 3 cut(s) 475, 737, 1616
EcoT14I CCWWGG 1 cut(s) 1227
EcoT22I ATGCAT 2 cut(s) 1179, 1370
EcoT38I GRGCYC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 1227
Esp3I CGTCTC 1 cut(s) 388
FaqI GGGAC 3 cut(s) 11, 1772, 1955
FauI CCCGC 2 cut(s) 36, 461
FbaI TGATCA 1 cut(s) 1158
FblI GTMKAC 1 cut(s) 1689
Fnu4HI GCNGC 5 cut(s) 264, 473, 492, 649, 1515
FokI GGATG 4 cut(s) 344, 820, 845, 1196
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 5 cut(s) 264, 473, 492, 649, 1515
FspBI CTAG 3 cut(s) 672, 954, 1400
GlaI GCGC 2 cut(s) 222, 652
GluI GCNGC 5 cut(s) 264, 473, 492, 649, 1515
HaeII RGCGCY 2 cut(s) 224, 654
HaeIII GGCC 1 cut(s) 1970
HapII CCGG 5 cut(s) 72, 1449, 1551, 1655, 1704
HhaI GCGC 2 cut(s) 223, 653
Hin6I GCGC 2 cut(s) 221, 651
HinP1I GCGC 2 cut(s) 221, 651
HincII GTYRAC 2 cut(s) 463, 1753
HindII GTYRAC 2 cut(s) 463, 1753
HindIII AAGCTT 2 cut(s) 1605, 1862
HpaI GTTAAC 1 cut(s) 463
HpaII CCGG 5 cut(s) 72, 1449, 1551, 1655, 1704
HphI GGTGA 8 cut(s) 26, 284, 430, 1100, 1243, 1718, 1772, 2009
Hpy166II GTNNAC 6 cut(s) 89, 135, 463, 1498, 1690, 1753
Hpy188I TCNGA 9 cut(s) 206, 331, 568, 681, 897, 908, 942, 1107, 1612
Hpy188III TCNNGA 8 cut(s) 52, 113, 299, 500, 766, 924, 1210, 1823
Hpy8I GTNNAC 6 cut(s) 89, 135, 463, 1498, 1690, 1753
HpyAV CCTTC 4 cut(s) 242, 807, 812, 1292
HpyCH4III ACNGT 5 cut(s) 65, 139, 541, 1141, 1566
HpyCH4IV ACGT 2 cut(s) 24, 85
HpyF10VI GCNNNNNNNGC 6 cut(s) 220, 263, 478, 786, 1460, 1511
HpyF3I CTNAG 9 cut(s) 38, 44, 203, 252, 565, 680, 696, 1382, 1501
HpySE526I ACGT 2 cut(s) 24, 85
HspAI GCGC 2 cut(s) 221, 651
Ksp22I TGATCA 1 cut(s) 1158
KspAI GTTAAC 1 cut(s) 463
Kzo9I GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
LmnI GCTCC 1 cut(s) 1460
Lsp1109I GCAGC 4 cut(s) 250, 459, 660, 1526
LweI GCATC 5 cut(s) 903, 1113, 1164, 1311, 1339
MaeI CTAG 3 cut(s) 672, 954, 1400
MaeII ACGT 2 cut(s) 24, 85
MaeIII GTNAC 6 cut(s) 535, 960, 1013, 1246, 1582, 1715
MalI GATC 8 cut(s) 78, 105, 944, 1160, 1332, 1356, 1821, 1841
MboI GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
MboII GAAGA 6 cut(s) 537, 603, 641, 1202, 1613, 1777
MflI RGATCY 1 cut(s) 1839
MhlI GDGCHC 3 cut(s) 91, 144, 211
MlsI TGGCCA 1 cut(s) 1970
MluNI TGGCCA 1 cut(s) 1970
MlyI GAGTC 3 cut(s) 196, 1217, 1695
MmeI TCCRAC 2 cut(s) 1074, 1596
Mox20I TGGCCA 1 cut(s) 1970
Mph1103I ATGCAT 2 cut(s) 1179, 1370
MroXI GAANNNNTTC 2 cut(s) 309, 638
MscI TGGCCA 1 cut(s) 1970
MseI TTAA 7 cut(s) 462, 687, 825, 930, 989, 1034, 1920
MslI CAYNNNNRTG 4 cut(s) 330, 416, 1182, 1491
Msp20I TGGCCA 1 cut(s) 1970
MspCI CTTAAG 1 cut(s) 988
MspI CCGG 5 cut(s) 72, 1449, 1551, 1655, 1704
MspR9I CCNGG 6 cut(s) 477, 739, 1449, 1551, 1618, 1656
Mva1269I GAATGC 2 cut(s) 853, 1434
MvaI CCWGG 3 cut(s) 477, 739, 1618
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 6 cut(s) 220, 263, 478, 786, 1460, 1511
NciI CCSGG 3 cut(s) 1449, 1551, 1656
NdeII GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
NlaIV GGNNCC 3 cut(s) 170, 1440, 1841
NmeAIII GCCGAG 2 cut(s) 190, 1264
NmuCI GTSAC 2 cut(s) 535, 1582
NsiI ATGCAT 2 cut(s) 1179, 1370
NspI RCATGY 1 cut(s) 131
NspV TTCGAA 1 cut(s) 1530
PcsI WCGNNNNNNNCGW 1 cut(s) 1725
PctI GAATGC 2 cut(s) 853, 1434
PdmI GAANNNNTTC 2 cut(s) 309, 638
PfeI GAWTC 7 cut(s) 179, 598, 920, 1316, 1641, 1901, 2003
PflFI GACNNNGTC 1 cut(s) 541
PflMI CCANNNNNTGG 1 cut(s) 1596
PfoI TCCNGGA 1 cut(s) 737
PkrI GCNGC 5 cut(s) 265, 474, 493, 650, 1516
PleI GAGTC 3 cut(s) 195, 1217, 1694
PpsI GAGTC 3 cut(s) 195, 1217, 1694
Psp124BI GAGCTC 1 cut(s) 211
Psp6I CCWGG 3 cut(s) 475, 737, 1616
PspGI CCWGG 3 cut(s) 475, 737, 1616
PspN4I GGNNCC 3 cut(s) 170, 1440, 1841
PspPI GGNCC 2 cut(s) 135, 438
PsrI GAACNNNNNNTAC 2 cut(s) 108, 140
PstNI CAGNNNCTG 1 cut(s) 895
PsuI RGATCY 1 cut(s) 1839
PsyI GACNNNGTC 1 cut(s) 541
RsaI GTAC 2 cut(s) 747, 1563
RsaNI GTAC 2 cut(s) 746, 1562
RseI CAYNNNNRTG 4 cut(s) 330, 416, 1182, 1491
SacI GAGCTC 1 cut(s) 211
SaqAI TTAA 7 cut(s) 462, 687, 825, 930, 989, 1034, 1920
SatI GCNGC 5 cut(s) 264, 473, 492, 649, 1515
Sau3AI GATC 8 cut(s) 76, 103, 942, 1158, 1330, 1354, 1819, 1839
Sau96I GGNCC 2 cut(s) 135, 438
SchI GAGTC 3 cut(s) 196, 1217, 1695
ScrFI CCNGG 6 cut(s) 477, 739, 1449, 1551, 1618, 1656
SduI GDGCHC 3 cut(s) 91, 144, 211
SfaNI GCATC 5 cut(s) 903, 1113, 1164, 1311, 1339
SfcI CTRYAG 1 cut(s) 1869
SfuI TTCGAA 1 cut(s) 1530
SinI GGWCC 2 cut(s) 135, 438
SmiMI CAYNNNNRTG 4 cut(s) 330, 416, 1182, 1491
SmlI CTYRAG 3 cut(s) 173, 988, 2008
SmoI CTYRAG 3 cut(s) 173, 988, 2008
SpeI ACTAGT 1 cut(s) 953
SsiI CCGC 4 cut(s) 29, 416, 468, 491
SspMI CTAG 3 cut(s) 672, 954, 1400
SstI GAGCTC 1 cut(s) 211
StyD4I CCNGG 6 cut(s) 475, 737, 1447, 1549, 1616, 1654
StyI CCWWGG 1 cut(s) 1227
TaaI ACNGT 5 cut(s) 65, 139, 541, 1141, 1566
TaiI ACGT 2 cut(s) 27, 88
TatI WGTACW 2 cut(s) 745, 1561
TauI GCSGC 1 cut(s) 494
TfiI GAWTC 7 cut(s) 179, 598, 920, 1316, 1641, 1901, 2003
Tru1I TTAA 7 cut(s) 462, 687, 825, 930, 989, 1034, 1920
Tru9I TTAA 7 cut(s) 462, 687, 825, 930, 989, 1034, 1920
TscAI CASTG 4 cut(s) 546, 1261, 1591, 1801
TseFI GTSAC 2 cut(s) 535, 1582
TseI GCWGC 4 cut(s) 263, 472, 648, 1514
Tsp45I GTSAC 2 cut(s) 535, 1582
TspDTI ATGAA 8 cut(s) 314, 318, 590, 831, 1060, 1311, 1408, 1954
TspGWI ACGGA 2 cut(s) 1148, 1343
TspRI CASTG 4 cut(s) 546, 1261, 1591, 1801
Tth111I GACNNNGTC 1 cut(s) 541
Van91I CCANNNNNTGG 1 cut(s) 1596
Vha464I CTTAAG 1 cut(s) 988
VneI GTGCAC 1 cut(s) 87
VpaK11BI GGWCC 2 cut(s) 135, 438
XagI CCTNNNNNAGG 1 cut(s) 480
XapI RAATTY 2 cut(s) 108, 1924
XceI RCATGY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 3 cut(s) 1060, 1963, 2027
XmiI GTMKAC 1 cut(s) 1689
XmnI GAANNNNTTC 2 cut(s) 309, 638
XspI CTAG 3 cut(s) 672, 954, 1400
Zsp2I ATGCAT 2 cut(s) 1179, 1370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.