MD05G1217200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004226
Physical Location & Seq
Reverse (-)
34796641 .. 34797615
975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1217200.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGTGTTTTTATCAATTTGTCCTCATTTTACGATTTTTCTGAATTTGGATTATGAAGAAAACAAAGGGGCTTTCTTGGTATTTTCAAATGTTTCACGAAACTGGCCCTCTCCCTTTATATATATAGACGACATGCTTTCAAAACTACACACCAACGTCCCGCCTCACCTGAGCCTGAGCCTTAGCTTCTTGAAAATCTACTGTGATTCCAGCGATTTGCAATGTGTACGCCGCCTGTTCGATCAAATTCCCGAACAAGACCTGCGTGCGTGGACCATGCTCATATCAGGCTACACACGACATGGGTTCCTCAAGGAATCCATAAATCTTTACGCTTCGCTCAGATTTCGGCCCGTTGTGCCCGATAATCTTCTGCTTTTGTCACTTCACGATGATGCAATTGGATTTGGGTTTCATTTGGATGTCTCTTTGGGCAATGCAATGGTCGATATGTTTGGAAAATGTAAATATGTTGATCTGGCGAGACTGGTTTTCGATGCCATGGCTGTGAAAGATGTGGTATCTTGGACCTCTTTTTGTTCTTGTTATGTTAACTGTGGGATGCCTAGACAAGGTTTGGAGGCATTTCACGAGATGGGGTTGAATGGAGTGAGACCCAATGTGGTAACAGTTTCTTCAATCCTACCTGCTTGCTCGGAGTTGAAAGATTTGAATTTGGGAAGTCTGATTCATGGGTTTGTGGTCAGACATGGAATGGAAGAGAATGTTTTCGTTAGCAGCGCCCTTGTAAATATCTATGCTAGTTGTCTGAGCATTAAACAAGCTCAGTTGGTATTTGATATGATGTCTAAACGAGATTTCGTGTCCTGGAATGTACTTCTAACTGCGTATTTTTCGAACAGAGATACCAAAAAAGGGTGTAACTTTTGCTGGCTATGTCTATGGGTCAGCTATGTCCATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

309

Amino Acids

34.95

Weight (kDa)

6.35

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 171 - 219 6.8e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 270, 655
AciI CCGC 2 cut(s) 161, 232
AcsI RAATTY 3 cut(s) 43, 246, 673
AfaI GTAC 2 cut(s) 228, 837
AfiI CCNNNNNNNGG 2 cut(s) 572, 876
AgsI TTSAA 7 cut(s) 87, 141, 193, 604, 639, 664, 673
AjnI CCWGG 1 cut(s) 827
AluBI AGCT 3 cut(s) 186, 785, 912
AluI AGCT 3 cut(s) 186, 785, 912
Alw26I GTCTC 3 cut(s) 430, 478, 607
AoxI GGCC 2 cut(s) 104, 350
ApeKI GCWGC 1 cut(s) 738
ApoI RAATTY 3 cut(s) 43, 246, 673
Asp700I GAANNNNTTC 1 cut(s) 728
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 4 cut(s) 105, 273, 351, 528
AsuHPI GGTGA 1 cut(s) 158
AsuII TTCGAA 1 cut(s) 857
AvaII GGWCC 2 cut(s) 273, 528
BaeGI GKGCMC 1 cut(s) 363
BauI CACGAG 1 cut(s) 590
BbvI GCAGC 1 cut(s) 750
BccI CCATC 1 cut(s) 589
BciT130I CCWGG 1 cut(s) 829
BcoDI GTCTC 3 cut(s) 430, 478, 607
BfaI CTAG 2 cut(s) 567, 762
BfoI RGCGCY 1 cut(s) 744
BfuAI ACCTGC 2 cut(s) 270, 655
BisI GCNGC 2 cut(s) 232, 739
BlsI GCNGC 2 cut(s) 233, 740
Bme1390I CCNGG 1 cut(s) 829
Bme18I GGWCC 2 cut(s) 273, 528
BmgT120I GGNCC 4 cut(s) 105, 273, 351, 528
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 1 cut(s) 829
BmsI GCATC 3 cut(s) 385, 487, 552
Bpu10I CCTNAGC 3 cut(s) 170, 176, 182
Bpu14I TTCGAA 1 cut(s) 857
BpuEI CTTGAG 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 607
BsaJI CCNNGG 2 cut(s) 501, 919
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 876
Bse1I ACTGG 2 cut(s) 107, 492
Bse3DI GCAATG 3 cut(s) 227, 442, 447
BseBI CCWGG 1 cut(s) 829
BseDI CCNNGG 2 cut(s) 501, 919
BseGI GGATG 2 cut(s) 427, 567
BseLI CCNNNNNNNGG 2 cut(s) 572, 876
BseMI GCAATG 3 cut(s) 227, 442, 447
BseMII CTCAG 5 cut(s) 161, 167, 355, 761, 800
BseNI ACTGG 2 cut(s) 107, 492
BseSI GKGCMC 1 cut(s) 363
BseXI GCAGC 1 cut(s) 750
BshFI GGCC 2 cut(s) 106, 352
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 2 cut(s) 572, 876
BsmAI GTCTC 3 cut(s) 430, 478, 607
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 2 cut(s) 106, 352
Bso31I GGTCTC 1 cut(s) 607
Bsp119I TTCGAA 1 cut(s) 857
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 2 cut(s) 241, 475
Bsp19I CCATGG 2 cut(s) 501, 919
BspACI CCGC 2 cut(s) 161, 232
BspANI GGCC 2 cut(s) 106, 352
BspCNI CTCAG 5 cut(s) 162, 168, 354, 762, 799
BspLI GGNNCC 1 cut(s) 308
BspMI ACCTGC 2 cut(s) 270, 655
BspT104I TTCGAA 1 cut(s) 857
BspTNI GGTCTC 1 cut(s) 607
BsrDI GCAATG 3 cut(s) 227, 442, 447
BsrI ACTGG 2 cut(s) 107, 492
BssECI CCNNGG 2 cut(s) 501, 919
BssMI GATC 2 cut(s) 241, 475
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 2 cut(s) 501, 919
Bst2BI CACGAG 1 cut(s) 590
Bst2UI CCWGG 1 cut(s) 829
Bst4CI ACNGT 3 cut(s) 203, 557, 631
Bst6I CTCTTC 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 857
BstC8I GCNNGC 3 cut(s) 267, 652, 893
BstDEI CTNAG 6 cut(s) 170, 176, 182, 341, 770, 786
BstDSI CCRYGG 2 cut(s) 501, 919
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 2 cut(s) 427, 567
BstH2I RGCGCY 1 cut(s) 744
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 2 cut(s) 244, 478
BstMAI GTCTC 3 cut(s) 430, 478, 607
BstMBI GATC 2 cut(s) 241, 475
BstMWI GCNNNNNNNGC 1 cut(s) 358
BstNI CCWGG 1 cut(s) 829
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 827
BstSLI GKGCMC 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 750
BsuRI GGCC 2 cut(s) 106, 352
BtgI CCRYGG 2 cut(s) 501, 919
BtsCI GGATG 2 cut(s) 427, 567
BveI ACCTGC 2 cut(s) 270, 655
Cac8I GCNNGC 3 cut(s) 267, 652, 893
CfoI GCGC 1 cut(s) 743
Cfr13I GGNCC 4 cut(s) 105, 273, 351, 528
Csp6I GTAC 2 cut(s) 227, 836
CviAII CATG 7 cut(s) 133, 277, 302, 502, 692, 710, 920
CviQI GTAC 2 cut(s) 227, 836
DdeI CTNAG 6 cut(s) 170, 176, 182, 341, 770, 786
DpnI GATC 2 cut(s) 243, 477
DpnII GATC 2 cut(s) 241, 475
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 501, 919
Eco31I GGTCTC 1 cut(s) 607
Eco47I GGWCC 2 cut(s) 273, 528
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoRII CCWGG 1 cut(s) 827
EcoT14I CCWWGG 2 cut(s) 501, 919
ErhI CCWWGG 2 cut(s) 501, 919
FaeI CATG 7 cut(s) 136, 280, 305, 505, 695, 713, 923
FaqI GGGAC 1 cut(s) 143
FatI CATG 7 cut(s) 132, 276, 301, 501, 691, 709, 919
FauI CCCGC 1 cut(s) 168
Fnu4HI GCNGC 2 cut(s) 232, 739
FokI GGATG 2 cut(s) 434, 574
Fsp4HI GCNGC 2 cut(s) 232, 739
FspBI CTAG 2 cut(s) 567, 762
GlaI GCGC 1 cut(s) 742
GluI GCNGC 2 cut(s) 232, 739
HaeII RGCGCY 1 cut(s) 744
HaeIII GGCC 2 cut(s) 106, 352
HhaI GCGC 1 cut(s) 743
Hin1II CATG 7 cut(s) 136, 280, 305, 505, 695, 713, 923
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 3 cut(s) 206, 317, 688
HpaI GTTAAC 1 cut(s) 553
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 3 cut(s) 227, 273, 553
Hpy188I TCNGA 6 cut(s) 42, 344, 658, 687, 707, 771
Hpy188III TCNNGA 5 cut(s) 96, 190, 251, 389, 590
Hpy8I GTNNAC 3 cut(s) 227, 273, 553
HpyCH4III ACNGT 3 cut(s) 203, 557, 631
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 3 cut(s) 220, 398, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 358
HpyF3I CTNAG 6 cut(s) 170, 176, 182, 341, 770, 786
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 7 cut(s) 136, 280, 305, 505, 695, 713, 923
HspAI GCGC 1 cut(s) 741
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 2 cut(s) 241, 475
Lsp1109I GCAGC 1 cut(s) 750
LweI GCATC 3 cut(s) 385, 487, 552
MaeI CTAG 2 cut(s) 567, 762
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 3 cut(s) 381, 625, 881
MalI GATC 2 cut(s) 243, 477
MboI GATC 2 cut(s) 241, 475
MboII GAAGA 4 cut(s) 68, 362, 627, 731
MfeI CAATTG 1 cut(s) 399
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 5 cut(s) 15, 43, 246, 399, 673
MnlI CCTC 6 cut(s) 33, 118, 174, 320, 541, 574
MroXI GAANNNNTTC 1 cut(s) 728
MseI TTAA 2 cut(s) 552, 777
MslI CAYNNNNRTG 3 cut(s) 393, 420, 506
MspR9I CCNGG 1 cut(s) 829
MunI CAATTG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 829
MwoI GCNNNNNNNGC 1 cut(s) 358
NcoI CCATGG 2 cut(s) 501, 919
NdeII GATC 2 cut(s) 241, 475
NlaIII CATG 7 cut(s) 136, 280, 305, 505, 695, 713, 923
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 1 cut(s) 381
NspI RCATGY 1 cut(s) 136
NspV TTCGAA 1 cut(s) 857
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 3 cut(s) 206, 317, 688
PfoI TCCNGGA 1 cut(s) 827
PkrI GCNGC 2 cut(s) 233, 740
Psp6I CCWGG 1 cut(s) 827
PspGI CCWGG 1 cut(s) 827
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 4 cut(s) 105, 273, 351, 528
RsaI GTAC 2 cut(s) 228, 837
RsaNI GTAC 2 cut(s) 227, 836
RseI CAYNNNNRTG 3 cut(s) 393, 420, 506
SaqAI TTAA 2 cut(s) 552, 777
SatI GCNGC 2 cut(s) 232, 739
Sau3AI GATC 2 cut(s) 241, 475
Sau96I GGNCC 4 cut(s) 105, 273, 351, 528
ScrFI CCNGG 1 cut(s) 829
SduI GDGCHC 1 cut(s) 363
SetI ASST 9 cut(s) 159, 171, 188, 264, 533, 577, 649, 787, 914
SfaNI GCATC 3 cut(s) 385, 487, 552
SfuI TTCGAA 1 cut(s) 857
SinI GGWCC 2 cut(s) 273, 528
SmiMI CAYNNNNRTG 3 cut(s) 393, 420, 506
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 5 cut(s) 15, 43, 246, 399, 673
SsiI CCGC 2 cut(s) 161, 232
SspMI CTAG 2 cut(s) 567, 762
StyD4I CCNGG 1 cut(s) 827
StyI CCWWGG 2 cut(s) 501, 919
TaaI ACNGT 3 cut(s) 203, 557, 631
TaiI ACGT 1 cut(s) 159
TaqI TCGA 4 cut(s) 240, 447, 495, 857
TasI AATT 5 cut(s) 15, 43, 246, 399, 673
TatI WGTACW 1 cut(s) 835
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 3 cut(s) 206, 317, 688
Tru1I TTAA 2 cut(s) 552, 777
Tru9I TTAA 2 cut(s) 552, 777
TseFI GTSAC 1 cut(s) 381
TseI GCWGC 1 cut(s) 738
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 3 cut(s) 69, 404, 680
VpaK11BI GGWCC 2 cut(s) 273, 528
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 3 cut(s) 43, 246, 673
XceI RCATGY 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 2 cut(s) 567, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.