Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001562

Physical Location & Seq

Genomic Coordinates Reverse (-)

35501475 .. 35508090

Gene Structure 6616 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1222500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGGCTTCTTTCTTGTCTGGTGATTCCTCTAATTATGGAATGAGTGAAAGTGGTTCCTATAAACAAGCTCATGACATATCAGAAGAAGGAGCCAATTGGTATTTTTCCAGGAAGGAAATTGAAGAAAACTCTCCATCTAAACAAGATGGCATTGACTTAAAAAAAGAGGCATATCTGCGCAAGTCATACTGTACTTTTTTACAAGACCTGGGGATGAGACTTAAAGTACCTCAGGTAACAATTGCGACGGCCATAATTTTCTGCCACCGATTCTTCCTTTGCCAATCCCATGCAAAGAATGATAGATGGACCATCGCAACGGTGTGTATGTTTCTCGCTGGGAAGGTTGAAGAAACTCCCAGGCCACTGAAAGATGTTATTCTTGTCTCATATGAAATTATTCATAAAAAGGATCCTGCAGCAGTTCATAGAATCAAGCAAAAGGAGGTATACGAACAGCAGAAAGAATTAATTTTACTAGGAGAGAGGGTTGTTCTTGGAACCCTTGCTTTCGATTTTAATGTACACCATCCGTATAAACCCCTGGTTGAGGCAATAAAGAAATTCAAAGTAGCTCAAAATGCCCTTGCCCAAGTTGCATGGAATTTTGTAAATGATGGACTTCGGACATCACTTTGCCTGCAATTTAAGCCCCACCACATTGCGGCTGGTGCCATTTTTCTTGCTGCTAAGTTCCTCAAAGTAAAGCTTCCATCAGATGGCGAGAAGGTCTGGTGGCAAGAGTTTGATGTCACCCCACGCCAATTGGAGGAGGTTAGCAATCAAATGCTGGAACTATATGAACAAAATCGGTTACCGCCTTCCAATGAAGTTGAAGGAAGTTCAGTGAGTGGTGCTAATCACCAGGCTTCTGCAAAACCTGCGACTAGCAATGATGAACAAGCAACTGGCAACAGTTACTTGCAGGCTGGAAGTACAAGACCTGGAACCTCAAAGCTAGCGTCGAGGCAAGGGTCTGAGCATTCATATGTTGAGAATGGTGGACCTGCTAGAATTACCCAAAGTCAAAATAATGACTACAGAAACGTTGAATTGAAAGGTGCCTTGGAGCAGAATATGAATGCTGAAGTGAAAGATATTCAACCCCCTGAAGCGCGGCACCAGGAGAAGATGGGTGAAGCTCAAAATGCATCAAGGTTTGTATCAGAGGGACGTAGTGAAGATGGTCAAGAAAGAGAGAGCAATATCGGGAAAGGTGAAACAAGGGAAGGATGGGAACCAAAGGATAAACATTTTGGCCGAAATCTGGATAATAGGCAAAGTGCACACGGCCGATCACCTCAGGAAGCTATCAAAAAAATTGACAAGGACAAGGTAAGGGCAGCACTGGAGAAACGAAGGAAATCTGTTGCCGATGTCACTCGAAAGACAGATGTTCTGGATGAGGATGATTTGATTGAGAGAGAACTGGAAGATGGTATTGAACTGGCTTCGGGGAGTGAGAAAAACAAGCGAGACCGGAGGCAAAGCTGGCCAAAGCCCTCAAATAGACCAGAACTTGGAGATGGACCACAATTAAACAATATAGAAGAAGGTGAGGTATCGGCACTTGATTCTGGACATGAATTTCATTCACCCAAATCAAGCAGTCGCAAGAGAAGGGCAGGAAGCCCACCAGATGATCACCGTGCTCACCGCAATCACAGTGATTATACAGATGATCACAACAGAGTGGGTCGGGCTGGCCACACAGAAAGAGATAACAAAAGACATGCACATGAGAATCATCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000079 GO:0000307 GO:0001932 GO:0001934 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0016538 GO:0019207 GO:0019219 GO:0019220 GO:0019222 GO:0019887 GO:0030234 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032991 GO:0033674 GO:0042325 GO:0042327 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0060255 GO:0061695 GO:0065007 GO:0065009 GO:0071900 GO:0071902 GO:0080090 GO:0098772 GO:1901407 GO:1901409 GO:1902494 GO:1902554 GO:1902680 GO:1902911 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko05202 map05202

Pfam Domains

Protein Families

Cyclin_N

Protein Analysis

585

Amino Acids

65.95

Weight (kDa)

6.8

Isoelectric Point (pI)

52.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyclin_N	PF00134	39 - 173	2.6e-15	Cyclin, N-terminal domain
CycT2-like_C	PF21797	177 - 264	2.3e-07	Cyclin-T2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001562)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19600 AT5G45190 AT5G45190 AT5G45190
fragaria_vesca	FvH4_3g15520 FvH4_3g15520 FvH4_3g15520 FvH4_3g15521 FvH4_3g15521
malus_domestica	MD05G1222500.v1.1 MD05G1222600.v1.1 MD10G1204100.v1.1 MD10G1204200.v1.1
prunus_persica	Prupe.4G137700_v2.0.a1 Prupe.4G137800_v2.0.a1
pyrus_communis	pycom05g20520 pycom10g17480 pycom10g17490
rosa_chinensis	RchiOBHm_Chr5g0026141 RchiOBHm_Chr5g0026151
rosa_laevigata	RLG00000032906 RLG00000032907
rosa_multiflora	Rmu_co8230253.1_g000001 Rmu_co8464075.1_g000001 Rmu_sc0002806.1_g000001 Rmu_sc0002909.1_g000016 Rmu_sc0009679.1_g000001 Rmu_sc0009679.1_g000002 Rmu_sc0029141.1_g000001
rosa_roxburghii	Rroxscaffold_1G00053050 Rroxscaffold_1G00053160 Rroxscaffold_1G00053170
rosa_rugosa	Rorug05G0091000.1
rosa_samantha	Rh2CG288400 Rh5AG183800 Rh5AG183900 Rh5AG184000 Rh5BG180600 Rh5BG180700 Rh5BG180800 Rh5CG199600 Rh5CG199700 Rh5CG199800 Rh5CG201400 Rh5DG182200 Rh5DG182300 Rh5DG182400
rosa_wichuraiana	Rw5G016640 Rw5G016650 Rw5G016810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	179
Acc36I	ACCTGC	2 cut(s)	889, 1015
AccB1I	GGYRCC	3 cut(s)	671, 1061, 1119
AccB7I	CCANNNNNTGG	2 cut(s)	719, 1520
AccI	GTMKAC	1 cut(s)	450
AccII	CGCG	1 cut(s)	1117
AciI	CCGC	4 cut(s)	665, 818, 1117, 1657
AclI	AACGTT	1 cut(s)	1047
AclWI	GGATC	2 cut(s)	407, 420
AcoI	YGGCCR	5 cut(s)	249, 1258, 1291, 1493, 1705
AcsI	RAATTY	3 cut(s)	563, 604, 1586
AcuI	CTGAAG	2 cut(s)	1107, 1131
AfaI	GTAC	4 cut(s)	193, 228, 525, 937
AfiI	CCNNNNNNNGG	6 cut(s)	550, 664, 719, 769, 1267, 1520
AgsI	TTSAA	8 cut(s)	122, 350, 568, 836, 1052, 1057, 1103, 1445
AjnI	CCWGG	7 cut(s)	107, 207, 359, 543, 864, 943, 1122
AjuI	GAANNNNNNNTTGG	2 cut(s)	1049, 1081
AluBI	AGCT	7 cut(s)	68, 575, 709, 958, 1142, 1310, 1491
AluI	AGCT	7 cut(s)	68, 575, 709, 958, 1142, 1310, 1491
Alw21I	GWGCWC	2 cut(s)	1288, 1654
Alw26I	GTCTC	3 cut(s)	211, 391, 1470
Alw44I	GTGCAC	1 cut(s)	1284
AlwI	GGATC	2 cut(s)	407, 420
AoxI	GGCC	6 cut(s)	249, 362, 1258, 1291, 1493, 1705
ApaLI	GTGCAC	1 cut(s)	1284
ApeKI	GCWGC	3 cut(s)	419, 686, 1343
ApoI	RAATTY	3 cut(s)	563, 604, 1586
ArsI	GACNNNNNNTTYG	2 cut(s)	947, 979
AseI	ATTAAT	1 cut(s)	470
Asp700I	GAANNNNTTC	1 cut(s)	399
AspLEI	GCGC	2 cut(s)	180, 1117
AspS9I	GGNCC	3 cut(s)	309, 1004, 1529
AsuNHI	GCTAGC	1 cut(s)	958
AvaII	GGWCC	3 cut(s)	309, 1004, 1529
AxyI	CCTNAGG	2 cut(s)	231, 1302
BaeGI	GKGCMC	1 cut(s)	1288
BalI	TGGCCA	2 cut(s)	1495, 1707
BamHI	GGATCC	1 cut(s)	412
BanI	GGYRCC	3 cut(s)	671, 1061, 1119
Bbv12I	GWGCWC	2 cut(s)	1288, 1654
BbvI	GCAGC	3 cut(s)	431, 673, 1355
BceAI	ACGGC	2 cut(s)	264, 1306
BciT130I	CCWGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
BclI	TGATCA	2 cut(s)	1642, 1681
BcoDI	GTCTC	3 cut(s)	211, 391, 1470
BfaI	CTAG	4 cut(s)	479, 888, 959, 1011
BfmI	CTRYAG	2 cut(s)	417, 1039
BfuAI	ACCTGC	2 cut(s)	889, 1015
BisI	GCNGC	5 cut(s)	420, 666, 687, 1118, 1344
BlsI	GCNGC	5 cut(s)	421, 667, 688, 1119, 1345
Bme1390I	CCNGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
Bme18I	GGWCC	3 cut(s)	309, 1004, 1529
BmgT120I	GGNCC	3 cut(s)	309, 1004, 1529
BmiI	GGNNCC	9 cut(s)	55, 91, 414, 502, 673, 949, 1063, 1121, 1239
BmrFI	CCNGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
BmsI	GCATC	1 cut(s)	1160
BmtI	GCTAGC	1 cut(s)	962
BpmI	CTGGAG	1 cut(s)	1370
BsaI	GGTCTC	1 cut(s)	1470
BsaJI	CCNNGG	4 cut(s)	208, 359, 543, 1065
BsaWI	WCCGGW	1 cut(s)	1479
BsaXI	ACNNNNNCTCC	2 cut(s)	474, 504
Bsc4I	CCNNNNNNNGG	6 cut(s)	550, 664, 719, 769, 1267, 1520
Bse1I	ACTGG	4 cut(s)	913, 1353, 1434, 1452
Bse21I	CCTNAGG	2 cut(s)	231, 1302
Bse3DI	GCAATG	2 cut(s)	660, 898
BseBI	CCWGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
BseDI	CCNNGG	4 cut(s)	208, 359, 543, 1065
BseGI	GGATG	5 cut(s)	219, 529, 1238, 1408, 1414
BseLI	CCNNNNNNNGG	6 cut(s)	550, 664, 719, 769, 1267, 1520
BseMI	GCAATG	2 cut(s)	660, 898
BseMII	CTCAG	3 cut(s)	245, 969, 1316
BseNI	ACTGG	4 cut(s)	913, 1353, 1434, 1452
BseRI	GAGGAG	1 cut(s)	785
BseSI	GKGCMC	1 cut(s)	1288
BseX3I	CGGCCG	1 cut(s)	1291
BseXI	GCAGC	3 cut(s)	431, 673, 1355
BseYI	CCCAGC	1 cut(s)	338
Bsh1236I	CGCG	1 cut(s)	1117
Bsh1285I	CGRYCG	1 cut(s)	1294
BshFI	GGCC	6 cut(s)	251, 364, 1260, 1293, 1495, 1707
BshNI	GGYRCC	3 cut(s)	671, 1061, 1119
BsiEI	CGRYCG	1 cut(s)	1294
BsiHKAI	GWGCWC	2 cut(s)	1288, 1654
BsiSI	CCGG	1 cut(s)	1480
BslFI	GGGAC	1 cut(s)	1185
BslI	CCNNNNNNNGG	6 cut(s)	550, 664, 719, 769, 1267, 1520
BsmAI	GTCTC	3 cut(s)	211, 391, 1470
BsmFI	GGGAC	1 cut(s)	1185
BsmI	GAATGC	2 cut(s)	982, 1087
BsnI	GGCC	6 cut(s)	251, 364, 1260, 1293, 1495, 1707
Bso31I	GGTCTC	1 cut(s)	1470
Bsp1286I	GDGCHC	2 cut(s)	1288, 1654
Bsp1407I	TGTACA	1 cut(s)	523
Bsp143I	GATC	4 cut(s)	412, 1295, 1642, 1681
BspACI	CCGC	4 cut(s)	665, 818, 1117, 1657
BspANI	GGCC	6 cut(s)	251, 364, 1260, 1293, 1495, 1707
BspCNI	CTCAG	3 cut(s)	244, 970, 1315
BspFNI	CGCG	1 cut(s)	1117
BspHI	TCATGA	1 cut(s)	70
BspLI	GGNNCC	9 cut(s)	55, 91, 414, 502, 673, 949, 1063, 1121, 1239
BspMAI	CTGCAG	1 cut(s)	421
BspMI	ACCTGC	2 cut(s)	889, 1015
BspOI	GCTAGC	1 cut(s)	962
BspPI	GGATC	2 cut(s)	407, 420
BspT107I	GGYRCC	3 cut(s)	671, 1061, 1119
BspTNI	GGTCTC	1 cut(s)	1470
BsrDI	GCAATG	2 cut(s)	660, 898
BsrGI	TGTACA	1 cut(s)	523
BsrI	ACTGG	4 cut(s)	913, 1353, 1434, 1452
BssECI	CCNNGG	4 cut(s)	208, 359, 543, 1065
BssMI	GATC	4 cut(s)	412, 1295, 1642, 1681
BssNAI	GTATAC	1 cut(s)	451
BssT1I	CCWWGG	1 cut(s)	1065
Bst1107I	GTATAC	1 cut(s)	451
Bst2UI	CCWGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
Bst4CI	ACNGT	5 cut(s)	191, 322, 917, 1649, 1667
BstAPI	GCANNNNNTGC	1 cut(s)	881
BstAUI	TGTACA	1 cut(s)	523
BstC8I	GCNNGC	5 cut(s)	641, 927, 960, 1493, 1705
BstDEI	CTNAG	4 cut(s)	231, 690, 978, 1302
BstEII	GGTNACC	1 cut(s)	813
BstENI	CCTNNNNNAGG	1 cut(s)	548
BstF5I	GGATG	5 cut(s)	219, 529, 1238, 1408, 1414
BstFNI	CGCG	1 cut(s)	1117
BstHHI	GCGC	2 cut(s)	180, 1117
BstKTI	GATC	4 cut(s)	415, 1298, 1645, 1684
BstMAI	GTCTC	3 cut(s)	211, 391, 1470
BstMBI	GATC	4 cut(s)	412, 1295, 1642, 1681
BstMCI	CGRYCG	1 cut(s)	1294
BstMWI	GCNNNNNNNGC	7 cut(s)	581, 596, 649, 671, 881, 1148, 1492
BstNI	CCWGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
BstNSI	RCATGY	1 cut(s)	1736
BstPI	GGTNACC	1 cut(s)	813
BstSCI	CCNGG	7 cut(s)	107, 207, 359, 543, 864, 943, 1122
BstSFI	CTRYAG	2 cut(s)	417, 1039
BstSLI	GKGCMC	1 cut(s)	1288
BstUI	CGCG	1 cut(s)	1117
BstV1I	GCAGC	3 cut(s)	431, 673, 1355
BstX2I	RGATCY	1 cut(s)	412
BstYI	RGATCY	1 cut(s)	412
BstZ17I	GTATAC	1 cut(s)	451
BstZI	CGGCCG	1 cut(s)	1291
Bsu36I	CCTNAGG	2 cut(s)	231, 1302
BsuRI	GGCC	6 cut(s)	251, 364, 1260, 1293, 1495, 1707
BtgZI	GCGATG	1 cut(s)	298
BtsCI	GGATG	5 cut(s)	219, 529, 1238, 1408, 1414
BtsIMutI	CAGTG	4 cut(s)	365, 852, 1346, 1672
BveI	ACCTGC	2 cut(s)	889, 1015
Cac8I	GCNNGC	5 cut(s)	641, 927, 960, 1493, 1705
CciI	TCATGA	1 cut(s)	70
CfoI	GCGC	2 cut(s)	180, 1117
Cfr13I	GGNCC	3 cut(s)	309, 1004, 1529
CseI	GACGC	1 cut(s)	951
Csp6I	GTAC	4 cut(s)	192, 227, 524, 936
CspCI	CAANNNNNGTGG	2 cut(s)	747, 782
CviAII	CATG	6 cut(s)	71, 290, 600, 1583, 1733, 1739
CviQI	GTAC	4 cut(s)	192, 227, 524, 936
DdeI	CTNAG	4 cut(s)	231, 690, 978, 1302
DpnI	GATC	4 cut(s)	414, 1297, 1644, 1683
DpnII	GATC	4 cut(s)	412, 1295, 1642, 1681
EaeI	YGGCCR	5 cut(s)	249, 1258, 1291, 1493, 1705
EagI	CGGCCG	1 cut(s)	1291
EclXI	CGGCCG	1 cut(s)	1291
Eco130I	CCWWGG	1 cut(s)	1065
Eco31I	GGTCTC	1 cut(s)	1470
Eco47I	GGWCC	3 cut(s)	309, 1004, 1529
Eco52I	CGGCCG	1 cut(s)	1291
Eco57I	CTGAAG	2 cut(s)	1107, 1131
Eco81I	CCTNAGG	2 cut(s)	231, 1302
Eco91I	GGTNACC	1 cut(s)	813
EcoNI	CCTNNNNNAGG	1 cut(s)	548
EcoO65I	GGTNACC	1 cut(s)	813
EcoRII	CCWGG	7 cut(s)	107, 207, 359, 543, 864, 943, 1122
EcoT14I	CCWWGG	1 cut(s)	1065
EcoT22I	ATGCAT	1 cut(s)	1153
ErhI	CCWWGG	1 cut(s)	1065
FaeI	CATG	6 cut(s)	74, 293, 603, 1586, 1736, 1742
FalI	AAGNNNNNCTT	2 cut(s)	693, 725
FaqI	GGGAC	1 cut(s)	1185
FatI	CATG	6 cut(s)	70, 289, 599, 1582, 1732, 1738
FauNDI	CATATG	2 cut(s)	391, 988
FbaI	TGATCA	2 cut(s)	1642, 1681
FblI	GTMKAC	1 cut(s)	450
Fnu4HI	GCNGC	5 cut(s)	420, 666, 687, 1118, 1344
FokI	GGATG	5 cut(s)	226, 516, 1245, 1415, 1421
Fsp4HI	GCNGC	5 cut(s)	420, 666, 687, 1118, 1344
FspBI	CTAG	4 cut(s)	479, 888, 959, 1011
FspI	TGCGCA	1 cut(s)	179
GlaI	GCGC	2 cut(s)	179, 1116
GluI	GCNGC	5 cut(s)	420, 666, 687, 1118, 1344
GsaI	CCCAGC	1 cut(s)	342
GsuI	CTGGAG	1 cut(s)	1370
HaeIII	GGCC	6 cut(s)	251, 364, 1260, 1293, 1495, 1707
HapII	CCGG	1 cut(s)	1480
HgaI	GACGC	1 cut(s)	951
HhaI	GCGC	2 cut(s)	180, 1117
Hin1II	CATG	6 cut(s)	74, 293, 603, 1586, 1736, 1742
Hin6I	GCGC	2 cut(s)	178, 1115
HinP1I	GCGC	2 cut(s)	178, 1115
HindIII	AAGCTT	1 cut(s)	707
HinfI	GANTC	5 cut(s)	23, 270, 432, 1574, 1744
HpaII	CCGG	1 cut(s)	1480
Hpy166II	GTNNAC	4 cut(s)	451, 526, 1004, 1286
Hpy188I	TCNGA	5 cut(s)	82, 627, 718, 979, 1168
Hpy188III	TCNNGA	7 cut(s)	71, 1190, 1210, 1268, 1304, 1400, 1578
Hpy8I	GTNNAC	4 cut(s)	451, 526, 1004, 1286
Hpy99I	CGWCG	2 cut(s)	250, 967
HpyCH4III	ACNGT	5 cut(s)	191, 322, 917, 1649, 1667
HpyCH4IV	ACGT	2 cut(s)	1047, 1174
HpyCH4V	TGCA	9 cut(s)	293, 419, 599, 643, 875, 925, 1151, 1286, 1736
HpyF10VI	GCNNNNNNNGC	7 cut(s)	581, 596, 649, 671, 881, 1148, 1492
HpyF3I	CTNAG	4 cut(s)	231, 690, 978, 1302
HpySE526I	ACGT	2 cut(s)	1047, 1174
Hsp92II	CATG	6 cut(s)	74, 293, 603, 1586, 1736, 1742
HspAI	GCGC	2 cut(s)	178, 1115
Ksp22I	TGATCA	2 cut(s)	1642, 1681
Kzo9I	GATC	4 cut(s)	412, 1295, 1642, 1681
LmnI	GCTCC	2 cut(s)	89, 1069
Lsp1109I	GCAGC	3 cut(s)	431, 673, 1355
LweI	GCATC	1 cut(s)	1160
MaeI	CTAG	4 cut(s)	479, 888, 959, 1011
MaeII	ACGT	2 cut(s)	1047, 1174
MaeIII	GTNAC	5 cut(s)	235, 751, 813, 917, 1378
MalI	GATC	4 cut(s)	414, 1297, 1644, 1683
MboI	GATC	4 cut(s)	412, 1295, 1642, 1681
MboII	GAAGA	8 cut(s)	95, 134, 265, 362, 1141, 1193, 1445, 1562
MfeI	CAATTG	3 cut(s)	94, 240, 764
MflI	RGATCY	1 cut(s)	412
MhlI	GDGCHC	2 cut(s)	1288, 1654
MlsI	TGGCCA	2 cut(s)	1495, 1707
MluNI	TGGCCA	2 cut(s)	1495, 1707
Mox20I	TGGCCA	2 cut(s)	1495, 1707
Mph1103I	ATGCAT	1 cut(s)	1153
MroXI	GAANNNNTTC	1 cut(s)	399
MscI	TGGCCA	2 cut(s)	1495, 1707
MseI	TTAA	6 cut(s)	158, 222, 470, 519, 648, 1538
MslI	CAYNNNNRTG	2 cut(s)	987, 1737
Msp20I	TGGCCA	2 cut(s)	1495, 1707
MspI	CCGG	1 cut(s)	1480
MspR9I	CCNGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
MunI	CAATTG	3 cut(s)	94, 240, 764
Mva1269I	GAATGC	2 cut(s)	982, 1087
MvaI	CCWGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
MvnI	CGCG	1 cut(s)	1117
MwoI	GCNNNNNNNGC	7 cut(s)	581, 596, 649, 671, 881, 1148, 1492
NdeI	CATATG	2 cut(s)	391, 988
NdeII	GATC	4 cut(s)	412, 1295, 1642, 1681
NheI	GCTAGC	1 cut(s)	958
NlaIII	CATG	6 cut(s)	74, 293, 603, 1586, 1736, 1742
NlaIV	GGNNCC	9 cut(s)	55, 91, 414, 502, 673, 949, 1063, 1121, 1239
NmuCI	GTSAC	2 cut(s)	751, 1378
NsbI	TGCGCA	1 cut(s)	179
NsiI	ATGCAT	1 cut(s)	1153
NspI	RCATGY	1 cut(s)	1736
PagI	TCATGA	1 cut(s)	70
PctI	GAATGC	2 cut(s)	982, 1087
PdmI	GAANNNNTTC	1 cut(s)	399
PfeI	GAWTC	5 cut(s)	23, 270, 432, 1574, 1744
PflMI	CCANNNNNTGG	2 cut(s)	719, 1520
PfoI	TCCNGGA	1 cut(s)	107
PkrI	GCNGC	5 cut(s)	421, 667, 688, 1119, 1345
PshBI	ATTAAT	1 cut(s)	470
Psp1406I	AACGTT	1 cut(s)	1047
Psp6I	CCWGG	7 cut(s)	107, 207, 359, 543, 864, 943, 1122
PspEI	GGTNACC	1 cut(s)	813
PspFI	CCCAGC	1 cut(s)	338
PspGI	CCWGG	7 cut(s)	107, 207, 359, 543, 864, 943, 1122
PspN4I	GGNNCC	9 cut(s)	55, 91, 414, 502, 673, 949, 1063, 1121, 1239
PspPI	GGNCC	3 cut(s)	309, 1004, 1529
PstI	CTGCAG	1 cut(s)	421
PsuI	RGATCY	1 cut(s)	412
RsaI	GTAC	4 cut(s)	193, 228, 525, 937
RsaNI	GTAC	4 cut(s)	192, 227, 524, 936
RseI	CAYNNNNRTG	2 cut(s)	987, 1737
SaqAI	TTAA	6 cut(s)	158, 222, 470, 519, 648, 1538
SatI	GCNGC	5 cut(s)	420, 666, 687, 1118, 1344
Sau3AI	GATC	4 cut(s)	412, 1295, 1642, 1681
Sau96I	GGNCC	3 cut(s)	309, 1004, 1529
ScrFI	CCNGG	7 cut(s)	109, 209, 361, 545, 866, 945, 1124
SduI	GDGCHC	2 cut(s)	1288, 1654
SfaNI	GCATC	1 cut(s)	1160
SfcI	CTRYAG	2 cut(s)	417, 1039
SinI	GGWCC	3 cut(s)	309, 1004, 1529
SmiMI	CAYNNNNRTG	2 cut(s)	987, 1737
SsiI	CCGC	4 cut(s)	665, 818, 1117, 1657
SspMI	CTAG	4 cut(s)	479, 888, 959, 1011
StyD4I	CCNGG	7 cut(s)	107, 207, 359, 543, 864, 943, 1122
StyI	CCWWGG	1 cut(s)	1065
TaaI	ACNGT	5 cut(s)	191, 322, 917, 1649, 1667
TaiI	ACGT	2 cut(s)	1050, 1177
TaqI	TCGA	3 cut(s)	513, 965, 1384
TatI	WGTACW	3 cut(s)	191, 523, 935
TauI	GCSGC	2 cut(s)	668, 1120
TfiI	GAWTC	5 cut(s)	23, 270, 432, 1574, 1744
Tru1I	TTAA	6 cut(s)	158, 222, 470, 519, 648, 1538
Tru9I	TTAA	6 cut(s)	158, 222, 470, 519, 648, 1538
TscAI	CASTG	4 cut(s)	372, 852, 1353, 1672
TseFI	GTSAC	2 cut(s)	751, 1378
TseI	GCWGC	3 cut(s)	419, 686, 1343
Tsp45I	GTSAC	2 cut(s)	751, 1378
TspGWI	ACGGA	1 cut(s)	522
TspRI	CASTG	4 cut(s)	372, 852, 1353, 1672
Van91I	CCANNNNNTGG	2 cut(s)	719, 1520
VneI	GTGCAC	1 cut(s)	1284
VpaK11BI	GGWCC	3 cut(s)	309, 1004, 1529
VspI	ATTAAT	1 cut(s)	470
XagI	CCTNNNNNAGG	1 cut(s)	548
XapI	RAATTY	3 cut(s)	563, 604, 1586
XceI	RCATGY	1 cut(s)	1736
XcmI	CCANNNNNNNNNTGG	1 cut(s)	665
XmiI	GTMKAC	1 cut(s)	450
XmnI	GAANNNNTTC	1 cut(s)	399
XspI	CTAG	4 cut(s)	479, 888, 959, 1011
Zsp2I	ATGCAT	1 cut(s)	1153

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.