Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003471

Physical Location & Seq

Genomic Coordinates Reverse (-)

36422003 .. 36427631

Gene Structure 5629 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1232900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGGCTTCCGAACCGGAGGACGAGAAGAGCTGGGAGGGCCTCCTCAGAAAGATGCTGCCTGCCGGCGCACCATTGCCCGACGAAGAGCACCTCGATTACTCCATCGCCATCGAGTACGAAGGTCCCCCGCTGCCCTACGACCTCCCTGAGGTTGATCCCGTTAACATCGACTCTCTCAATCTTCACTCTTCTTCCGTGGCCGCCGGTTCGGTTGAAGCCCCGTCTTCGATTCCGGTGGCTTCCCCGGTTGGTCAGAAGGTCTCGCGGTTCAATCGGGCGAGGAACGGCGGCGTTGTGAAGGAGACAAGTAGAACGGTGTCGTTTCCGACTCCGAGGACGCAACCGGAGCTGCGGAACAGCAGGGAAGGATCGGAATTTGAAGGAGCGGAGGAAGGTTTCATCTCTGAGTTGCCGGTTCAAGACTCGAACTTGGAGCAAAAACCGATCGGCGGCGCCACCGGAGGAAAGAGGGCGGCTGTCGTGACTTTCAATGAACCAAGAGATTTGGAGAACGAGGACGACCCCGAAAACGATAACGACGGCGGTAGGTCGTCGTCGCCGCAATCGAGCGCCGCCACCGAACAGGTGGGGTCGCCGGTTGCATGGGCCTCTTCGCCCGGGAGGCGATCGAGTAAGAGAGGAATTTGCAGCAGGTGCGGGAAAGGGAACAGATTGAAGGAGAGAGAATGGTGTTTGGTGTGCGACGCCAAGTACTGCAGTAATTGCCTTCTGAAGGCGATGGGGTCGATGCCGGAGGGGAGGAAATGTGTGAGCTGCATTGGGCAGCCGATAGACGAGTCGAAAAGGTCGAGCTTGGGCAAGTGTTCGAGGATTCTGTCGAGGGTTTGCAGCCCTTTGGAGATTGGGCAGATAATGAGGGCCGAGAAGGAGTGCCCCGCCAATCAGCTCCGGCCGGAGCAGTTGGTTGTGAATGGGAGGCAGCTGAGGGAAGAAGAACTGGCTGAGATTTTGGGATGTGAATTGCCTCCTCAGAAGTTGAAGCCGGGGAGGTATTGGTACGATAAGGATTCTGGTCTTTGGGGAAAGGAAGGTGAGAAGCCGGGTAGGATCATTAGCTCGAAACTCAATGTTGGGGGTAAGCTTAGGTCCGATGCTAGCAATGGGAACACCAAAGTGTTCATGAATGGTCGAGAGATCACAAAGATTGAGCGTAGGATGTTGAAGCTGGCCAACGTGCAGTGCCCTCCGGATACTCATTTCTGGGTGTATGATGACGGCTCTTATGAGGAGGAAGGTCAAAATAACATTAAAGGGAATATATGGGGAAAGGCATCCACTCGTTTCATTTGTTCATTATTCTCATTGCCCGTACCATCTGGTAATCAAAGTGGGGCAAAAGAAGATCCTACGACACCTTCAAGCAGGTCAGTCCCCGAATACTTGGAACAGGGAAGAGTTCAGAAACTTCTGTTATTTGGCTTGGAGGGATCTGGAACTAGCACAATCTTCAAGCAGGCTAAGTTATTATATGGGAACAAGTTCACTTCCGAAGAGCTACAAAACATCAGACTCATGATTCAAAGCAATATGTATAAGTATCTAAGTATATTACTTGAGGGCCGAGAACGTTTTGAAGAGGAGGCATTGACAGAGAATAGGATATCTTTGTCAGCTGCTCATGATTCCCCTTCAGTTGATGAAAGTGAGCAATGCATCTATGCAATCAACCAAAGGTTCAAGCACTTTTCTGACTGGTTACTGGATATCATGGCAACCGGAGACTTGGATGCATTCTTTCCTGCTGCCACACGTGAGTATGCTCCTATTGTGTATGAAGTGTGGAGAGATCCTGCCATCCAGGAAACATACAAAAGGAGGGAGGAATTACACTGTTTTCCTGAAGTTGCCAAATATTTCTTAGATAGGGCTATAGAAATATCAAGTAACGAGTATGAGCCTTCTGAGAAGGACATTTTATATGCTGAGGGTGTTACTCAGAGCAATGGACTTGCCTCTATGGAATTTTCATTTGATGACAGAAGTGCCATGTCTGAGCTATACAATGAAACTTATGAATACCCCCTTGCCTTGACTAAGTATCAACTAATTCGCATCAATTCCAAGGGACTGAGTGAAGGTTTCAAATGGCTCGAAATGTTTGAAGACATGAGGGCAGTGATATTTTGTGTTGCGTTGAGTGACTATGATCAAATGTGGGCCGACGGCAATGGTCTTCTTCATAATAAAATGCTGGCAAGCAGAGATTTGTTTGAGAGTTTGGTACGGCACCCTTGTTTCAGAGACACCGCATTTGTGCTCTTTCTTAACAAATATGATGCCTTTGAAGGCAAGATAAACCAGGTTCCATTGTCTGCCTGCGAGTGGCTAAGGGACTTCAGTCCTGTTAAGCCCCATGGTAATAATCAGTCTATGGCACAGCAAGCATATTATTATGTGGCGGTGAAATTCAAAGAGCTCTATTCATCCATAACTGGCGAAAAGTTGTTTGTGGCACAGACCCGAGCTTGGGAGGGTGCCTCAGTTGACGAGGCATTCAAGTACGTGAGGGAGGTCCTTAAGTGGGATGAAGAAAAGAATGACAATTTCTTTGCAATAAACGGGGACGACTCATTTTACAGCACAGAGATGAGTTCTCCTCCTGTCAGGCAGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200

Pfam Domains

Protein Families

G-alpha

Protein Analysis

879

Amino Acids

98.72

Weight (kDa)

5.08

Isoelectric Point (pI)

56.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	473 - 845	1.6e-72	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003471)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g17740 FvH4_5g17740
malus_domestica	MD06G1232900.v1.1 MD14G1239900.v1.1
prunus_persica	Prupe.5G237500_v2.0.a1 Prupe.5G237500_v2.0.a1
pyrus_communis	pycom06g20790 pycom14g20150
rosa_chinensis	RchiOBHm_Chr5g0016271 RchiOBHm_Chr7g0177881
rosa_laevigata	RLG00000003463 RLG00000005494 RLG00000019305 RLG00000035269
rosa_multiflora	Rmu_sc0000141.1_g000001 Rmu_sc0004510.1_g000005 Rmu_sc0006214.1_g000003 Rmu_sc0006997.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129380 Rroxscaffold_3G00275470
rosa_rugosa	Rorug04G0425800 Rorug06G0410700 Rorug06G0410800
rosa_samantha	Rh1CG219000 Rh4CG242300 Rh5AG324200 Rh5CG136200 Rh7AG009900 Rh7AG127600 Rh7BG010000 Rh7BG128600 Rh7BG129500 Rh7CG130400 Rh7CG131800 Rh7DG010000
rosa_wichuraiana	Rw7G000830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	642
Acc36I	ACCTGC	2 cut(s)	642, 1374
AccB1I	GGYRCC	3 cut(s)	452, 2247, 2495
AccBSI	CCGCTC	1 cut(s)	386
AccII	CGCG	1 cut(s)	265
AccIII	TCCGGA	1 cut(s)	1207
AclI	AACGTT	1 cut(s)	1588
AclWI	GGATC	6 cut(s)	149, 376, 1076, 1358, 1456, 1802
AcoI	YGGCCR	3 cut(s)	198, 911, 1188
AcsI	RAATTY	4 cut(s)	374, 642, 1982, 2426
AcuI	CTGAAG	4 cut(s)	752, 1635, 1881, 2341
AcvI	CACGTG	1 cut(s)	1772
AcyI	GRCGYC	2 cut(s)	453, 705
AfaI	GTAC	6 cut(s)	116, 713, 1019, 1332, 2244, 2522
AfiI	CCNNNNNNNGG	7 cut(s)	148, 449, 733, 2343, 2487, 2488, 2542
AflII	CTTAAG	1 cut(s)	2537
AflIII	ACRYGT	1 cut(s)	1769
AjnI	CCWGG	2 cut(s)	1818, 2319
AleI	CACNNNNGTG	1 cut(s)	1133
Alw21I	GWGCWC	3 cut(s)	90, 2280, 2439
Alw26I	GTCTC	4 cut(s)	265, 296, 1734, 2256
AlwI	GGATC	6 cut(s)	149, 376, 1076, 1358, 1456, 1802
Ama87I	CYCGRG	2 cut(s)	617, 2481
Aor13HI	TCCGGA	1 cut(s)	1207
AoxI	GGCC	8 cut(s)	37, 198, 606, 879, 911, 1188, 1579, 2178
ApoI	RAATTY	4 cut(s)	374, 642, 1982, 2426
ArsI	GACNNNNNNTTYG	4 cut(s)	2255, 2287, 2552, 2584
Asp700I	GAANNNNTTC	1 cut(s)	1499
AspLEI	GCGC	3 cut(s)	68, 455, 572
AspS9I	GGNCC	8 cut(s)	37, 122, 606, 879, 1107, 1579, 2178, 2533
AsuC2I	CCSGG	5 cut(s)	245, 618, 619, 1005, 1062
AsuHPI	GGTGA	2 cut(s)	1064, 2434
AsuNHI	GCTAGC	1 cut(s)	1115
AvaI	CYCGRG	2 cut(s)	617, 2481
AvaII	GGWCC	3 cut(s)	122, 1107, 2533
AxyI	CCTNAGG	1 cut(s)	147
BaeGI	GKGCMC	2 cut(s)	896, 1205
BalI	TGGCCA	1 cut(s)	1190
BanI	GGYRCC	3 cut(s)	452, 2247, 2495
BanII	GRGCYC	1 cut(s)	2439
BbrPI	CACGTG	1 cut(s)	1772
BbsI	GAAGAC	3 cut(s)	216, 2130, 2186
Bbv12I	GWGCWC	3 cut(s)	90, 2280, 2439
BbvCI	CCTCAGC	2 cut(s)	944, 1944
BccI	CCATC	5 cut(s)	110, 116, 733, 1342, 1823
BceAI	ACGGC	5 cut(s)	301, 556, 1252, 2200, 2261
BciT130I	CCWGG	2 cut(s)	1820, 2321
BciVI	GTATCC	1 cut(s)	1204
BclI	TGATCA	1 cut(s)	2167
BcnI	CCSGG	5 cut(s)	245, 618, 619, 1005, 1062
BcoDI	GTCTC	4 cut(s)	265, 296, 1734, 2256
BfaI	CTAG	2 cut(s)	1116, 1458
BfmI	CTRYAG	2 cut(s)	715, 1890
BfoI	RGCGCY	2 cut(s)	456, 573
BfrI	CTTAAG	1 cut(s)	2537
BfuAI	ACCTGC	2 cut(s)	642, 1374
BfuI	GTATCC	1 cut(s)	1204
BglI	GCCNNNNNGGC	1 cut(s)	622
BmcAI	AGTACT	1 cut(s)	713
Bme1390I	CCNGG	7 cut(s)	245, 618, 619, 1005, 1062, 1820, 2321
Bme18I	GGWCC	3 cut(s)	122, 1107, 2533
BmeT110I	CYCGRG	2 cut(s)	617, 2481
BmgT120I	GGNCC	8 cut(s)	37, 122, 606, 879, 1107, 1579, 2178, 2533
BmiI	GGNNCC	5 cut(s)	124, 454, 2249, 2325, 2497
BmrFI	CCNGG	7 cut(s)	245, 618, 619, 1005, 1062, 1820, 2321
BmsI	GCATC	8 cut(s)	42, 738, 1102, 1301, 1683, 1738, 2082, 2287
BmtI	GCTAGC	1 cut(s)	1119
BoxI	GACNNNNGTC	1 cut(s)	2358
BpiI	GAAGAC	3 cut(s)	216, 2130, 2186
BplI	GAGNNNNNCTC	4 cut(s)	2483, 2515, 2602, 2634
Bpu10I	CCTNAGC	4 cut(s)	944, 1103, 1944, 2348
BpuEI	CTTGAG	1 cut(s)	1595
BpuMI	CCSGG	5 cut(s)	245, 618, 619, 1005, 1062
BsaAI	YACGTR	2 cut(s)	1772, 2524
BsaHI	GRCGYC	2 cut(s)	453, 705
BsaI	GGTCTC	1 cut(s)	265
BsaJI	CCNNGG	7 cut(s)	195, 243, 332, 617, 1004, 2082, 2374
BsaWI	WCCGGW	6 cut(s)	13, 232, 343, 458, 1207, 1736
BsaXI	ACNNNNNCTCC	2 cut(s)	751, 781
Bsc4I	CCNNNNNNNGG	7 cut(s)	148, 449, 733, 2343, 2487, 2488, 2542
Bse118I	RCCGGY	4 cut(s)	62, 203, 412, 595
Bse1I	ACTGG	4 cut(s)	963, 1718, 1725, 2458
Bse21I	CCTNAGG	1 cut(s)	147
Bse3DI	GCAATG	6 cut(s)	71, 1126, 1322, 1676, 1969, 2194
BseAI	TCCGGA	1 cut(s)	1207
BseBI	CCWGG	2 cut(s)	1820, 2321
BseDI	CCNNGG	7 cut(s)	195, 243, 332, 617, 1004, 2082, 2374
BseGI	GGATG	7 cut(s)	980, 1182, 1292, 1753, 1815, 2444, 2551
BseLI	CCNNNNNNNGG	7 cut(s)	148, 449, 733, 2343, 2487, 2488, 2542
BseMI	GCAATG	6 cut(s)	71, 1126, 1322, 1676, 1969, 2194
BseNI	ACTGG	4 cut(s)	963, 1718, 1725, 2458
BseRI	GAGGAG	5 cut(s)	32, 978, 1262, 1613, 2607
BseSI	GKGCMC	2 cut(s)	896, 1205
BseX3I	CGGCCG	1 cut(s)	911
BseYI	CCCAGC	1 cut(s)	30
BsgI	GTGCAG	1 cut(s)	1217
Bsh1236I	CGCG	1 cut(s)	265
Bsh1285I	CGRYCG	3 cut(s)	447, 629, 914
BshFI	GGCC	8 cut(s)	39, 200, 608, 881, 913, 1190, 1581, 2180
BshNI	GGYRCC	3 cut(s)	452, 2247, 2495
BsiEI	CGRYCG	3 cut(s)	447, 629, 914
BsiHKAI	GWGCWC	3 cut(s)	90, 2280, 2439
BsiHKCI	CYCGRG	2 cut(s)	617, 2481
BslFI	GGGAC	5 cut(s)	108, 1376, 2100, 2366, 2597
BslI	CCNNNNNNNGG	7 cut(s)	148, 449, 733, 2343, 2487, 2488, 2542
BsmAI	GTCTC	4 cut(s)	265, 296, 1734, 2256
BsmFI	GGGAC	5 cut(s)	108, 1376, 2100, 2366, 2597
BsmI	GAATGC	2 cut(s)	1751, 2513
BsnI	GGCC	8 cut(s)	39, 200, 608, 881, 913, 1190, 1581, 2180
Bso31I	GGTCTC	1 cut(s)	265
BsoBI	CYCGRG	2 cut(s)	617, 2481
Bsp1286I	GDGCHC	5 cut(s)	90, 896, 1205, 2280, 2439
Bsp13I	TCCGGA	1 cut(s)	1207
Bsp19I	CCATGG	1 cut(s)	2374
BspANI	GGCC	8 cut(s)	39, 200, 608, 881, 913, 1190, 1581, 2180
BspEI	TCCGGA	1 cut(s)	1207
BspFNI	CGCG	1 cut(s)	265
BspHI	TCATGA	3 cut(s)	1140, 1533, 1639
BspLI	GGNNCC	5 cut(s)	124, 454, 2249, 2325, 2497
BspMAI	CTGCAG	1 cut(s)	719
BspMI	ACCTGC	2 cut(s)	642, 1374
BspOI	GCTAGC	1 cut(s)	1119
BspPI	GGATC	6 cut(s)	149, 376, 1076, 1358, 1456, 1802
BspQI	GCTCTTC	3 cut(s)	20, 78, 1506
BspT107I	GGYRCC	3 cut(s)	452, 2247, 2495
BspTI	CTTAAG	1 cut(s)	2537
BspTNI	GGTCTC	1 cut(s)	265
BsrBI	CCGCTC	1 cut(s)	386
BsrDI	GCAATG	6 cut(s)	71, 1126, 1322, 1676, 1969, 2194
BsrFI	RCCGGY	4 cut(s)	62, 203, 412, 595
BsrI	ACTGG	4 cut(s)	963, 1718, 1725, 2458
BssAI	RCCGGY	4 cut(s)	62, 203, 412, 595
BssECI	CCNNGG	7 cut(s)	195, 243, 332, 617, 1004, 2082, 2374
BssNI	GRCGYC	2 cut(s)	453, 705
BssT1I	CCWWGG	2 cut(s)	2082, 2374
Bst2UI	CCWGG	2 cut(s)	1820, 2321
Bst4CI	ACNGT	2 cut(s)	316, 1853
Bst6I	CTCTTC	7 cut(s)	20, 78, 193, 616, 1408, 1506, 1590
BstACI	GRCGYC	2 cut(s)	453, 705
BstAFI	CTTAAG	1 cut(s)	2537
BstAPI	GCANNNNNTGC	2 cut(s)	654, 723
BstBAI	YACGTR	2 cut(s)	1772, 2524
BstC8I	GCNNGC	9 cut(s)	60, 64, 1117, 1188, 1476, 2214, 2218, 2338, 2403
BstDSI	CCRYGG	2 cut(s)	195, 2374
BstENI	CCTNNNNNAGG	2 cut(s)	146, 731
BstF5I	GGATG	7 cut(s)	980, 1182, 1292, 1753, 1815, 2444, 2551
BstFNI	CGCG	1 cut(s)	265
BstH2I	RGCGCY	2 cut(s)	456, 573
BstHHI	GCGC	3 cut(s)	68, 455, 572
BstMAI	GTCTC	4 cut(s)	265, 296, 1734, 2256
BstMCI	CGRYCG	3 cut(s)	447, 629, 914
BstMWI	GCNNNNNNNGC	6 cut(s)	36, 346, 622, 654, 723, 2402
BstNI	CCWGG	2 cut(s)	1820, 2321
BstPAI	GACNNNNGTC	1 cut(s)	2358
BstSCI	CCNGG	7 cut(s)	243, 616, 617, 1003, 1060, 1818, 2319
BstSFI	CTRYAG	2 cut(s)	715, 1890
BstSLI	GKGCMC	2 cut(s)	896, 1205
BstUI	CGCG	1 cut(s)	265
BstV2I	GAAGAC	3 cut(s)	216, 2130, 2186
BstX2I	RGATCY	3 cut(s)	1363, 1448, 1807
BstYI	RGATCY	3 cut(s)	1363, 1448, 1807
BstZI	CGGCCG	1 cut(s)	911
Bsu36I	CCTNAGG	1 cut(s)	147
BsuI	GTATCC	1 cut(s)	1204
BsuRI	GGCC	8 cut(s)	39, 200, 608, 881, 913, 1190, 1581, 2180
BtgI	CCRYGG	2 cut(s)	195, 2374
BtgZI	GCGATG	2 cut(s)	88, 752
BtsCI	GGATG	7 cut(s)	980, 1182, 1292, 1753, 1815, 2444, 2551
BtsI	GCAGTG	2 cut(s)	1205, 2142
BtsIMutI	CAGTG	3 cut(s)	1205, 1849, 2142
BveI	ACCTGC	2 cut(s)	642, 1374
Cac8I	GCNNGC	9 cut(s)	60, 64, 1117, 1188, 1476, 2214, 2218, 2338, 2403
CciI	TCATGA	3 cut(s)	1140, 1533, 1639
CfoI	GCGC	3 cut(s)	68, 455, 572
Cfr10I	RCCGGY	4 cut(s)	62, 203, 412, 595
Cfr13I	GGNCC	8 cut(s)	37, 122, 606, 879, 1107, 1579, 2178, 2533
Cfr9I	CCCGGG	1 cut(s)	617
CseI	GACGC	2 cut(s)	346, 713
CsiI	ACCWGGT	1 cut(s)	2319
Csp6I	GTAC	6 cut(s)	115, 712, 1018, 1331, 2243, 2521
CviAII	CATG	8 cut(s)	603, 1141, 1534, 1640, 1729, 2008, 2128, 2375
CviQI	GTAC	6 cut(s)	115, 712, 1018, 1331, 2243, 2521
DinI	GGCGCC	1 cut(s)	454
EaeI	YGGCCR	3 cut(s)	198, 911, 1188
EagI	CGGCCG	1 cut(s)	911
Eam1104I	CTCTTC	7 cut(s)	20, 78, 193, 616, 1408, 1506, 1590
EarI	CTCTTC	7 cut(s)	20, 78, 193, 616, 1408, 1506, 1590
Ecl136II	GAGCTC	1 cut(s)	2437
EclXI	CGGCCG	1 cut(s)	911
Eco130I	CCWWGG	2 cut(s)	2082, 2374
Eco24I	GRGCYC	1 cut(s)	2439
Eco31I	GGTCTC	1 cut(s)	265
Eco32I	GATATC	2 cut(s)	1623, 1726
Eco47I	GGWCC	3 cut(s)	122, 1107, 2533
Eco52I	CGGCCG	1 cut(s)	911
Eco53kI	GAGCTC	1 cut(s)	2437
Eco57I	CTGAAG	4 cut(s)	752, 1635, 1881, 2341
Eco72I	CACGTG	1 cut(s)	1772
Eco81I	CCTNAGG	1 cut(s)	147
Eco88I	CYCGRG	2 cut(s)	617, 2481
EcoICRI	GAGCTC	1 cut(s)	2437
EcoNI	CCTNNNNNAGG	2 cut(s)	146, 731
EcoO109I	RGGNCCY	3 cut(s)	37, 122, 2533
EcoRII	CCWGG	2 cut(s)	1818, 2319
EcoRV	GATATC	2 cut(s)	1623, 1726
EcoT14I	CCWWGG	2 cut(s)	2082, 2374
EcoT22I	ATGCAT	2 cut(s)	1676, 1753
EcoT38I	GRGCYC	1 cut(s)	2439
EgeI	GGCGCC	1 cut(s)	454
EheI	GGCGCC	1 cut(s)	454
ErhI	CCWWGG	2 cut(s)	2082, 2374
FaeI	CATG	8 cut(s)	606, 1144, 1537, 1643, 1732, 2011, 2131, 2378
FaqI	GGGAC	5 cut(s)	108, 1376, 2100, 2366, 2597
FatI	CATG	8 cut(s)	602, 1140, 1533, 1639, 1728, 2007, 2127, 2374
FauI	CCCGC	3 cut(s)	135, 650, 904
FbaI	TGATCA	1 cut(s)	2167
FokI	GGATG	7 cut(s)	987, 1189, 1279, 1760, 1802, 2431, 2558
FriOI	GRGCYC	1 cut(s)	2439
FspBI	CTAG	2 cut(s)	1116, 1458
GlaI	GCGC	3 cut(s)	67, 454, 571
GsaI	CCCAGC	1 cut(s)	34
HaeII	RGCGCY	2 cut(s)	456, 573
HaeIII	GGCC	8 cut(s)	39, 200, 608, 881, 913, 1190, 1581, 2180
HgaI	GACGC	2 cut(s)	346, 713
HhaI	GCGC	3 cut(s)	68, 455, 572
Hin1I	GRCGYC	2 cut(s)	453, 705
Hin1II	CATG	8 cut(s)	606, 1144, 1537, 1643, 1732, 2011, 2131, 2378
Hin6I	GCGC	3 cut(s)	66, 453, 570
HinP1I	GCGC	3 cut(s)	66, 453, 570
HincII	GTYRAC	2 cut(s)	163, 2506
HindII	GTYRAC	2 cut(s)	163, 2506
HindIII	AAGCTT	1 cut(s)	1100
HpaI	GTTAAC	1 cut(s)	163
HphI	GGTGA	2 cut(s)	1064, 2434
Hpy166II	GTNNAC	3 cut(s)	163, 1503, 2506
Hpy188III	TCNNGA	9 cut(s)	419, 481, 1141, 1151, 1208, 1452, 1534, 1640, 1859
Hpy8I	GTNNAC	3 cut(s)	163, 1503, 2506
Hpy99I	CGWCG	6 cut(s)	83, 542, 556, 559, 707, 2186
HpyCH4III	ACNGT	2 cut(s)	316, 1853
HpyCH4IV	ACGT	4 cut(s)	1194, 1588, 1771, 2523
HpyF10VI	GCNNNNNNNGC	6 cut(s)	36, 346, 622, 654, 723, 2402
HpySE526I	ACGT	4 cut(s)	1194, 1588, 1771, 2523
Hsp92I	GRCGYC	2 cut(s)	453, 705
Hsp92II	CATG	8 cut(s)	606, 1144, 1537, 1643, 1732, 2011, 2131, 2378
HspAI	GCGC	3 cut(s)	66, 453, 570
KasI	GGCGCC	1 cut(s)	452
Kpn2I	TCCGGA	1 cut(s)	1207
KroI	GCCGGC	1 cut(s)	62
KroNI	GCCGGC	1 cut(s)	64
Ksp22I	TGATCA	1 cut(s)	2167
KspAI	GTTAAC	1 cut(s)	163
LguI	GCTCTTC	3 cut(s)	20, 78, 1506
LmnI	GCTCC	6 cut(s)	346, 383, 433, 912, 916, 1786
LweI	GCATC	8 cut(s)	42, 738, 1102, 1301, 1683, 1738, 2082, 2287
MabI	ACCWGGT	1 cut(s)	2319
MaeI	CTAG	2 cut(s)	1116, 1458
MaeII	ACGT	4 cut(s)	1194, 1588, 1771, 2523
MaeIII	GTNAC	5 cut(s)	481, 1716, 1904, 1951, 2159
MbiI	CCGCTC	1 cut(s)	386
MflI	RGATCY	3 cut(s)	1363, 1448, 1807
MhlI	GDGCHC	5 cut(s)	90, 896, 1205, 2280, 2439
MlsI	TGGCCA	1 cut(s)	1190
MluNI	TGGCCA	1 cut(s)	1190
Mly113I	GGCGCC	1 cut(s)	453
MlyI	GAGTC	6 cut(s)	164, 322, 416, 806, 1524, 2582
MmeI	TCCRAC	1 cut(s)	350
Mox20I	TGGCCA	1 cut(s)	1190
Mph1103I	ATGCAT	2 cut(s)	1676, 1753
MroI	TCCGGA	1 cut(s)	1207
MroNI	GCCGGC	1 cut(s)	62
MroXI	GAANNNNTTC	1 cut(s)	1499
MscI	TGGCCA	1 cut(s)	1190
MseI	TTAA	5 cut(s)	162, 1269, 2286, 2367, 2538
MslI	CAYNNNNRTG	2 cut(s)	1133, 2606
Msp20I	TGGCCA	1 cut(s)	1190
MspA1I	CMGCKG	3 cut(s)	130, 943, 1634
MspCI	CTTAAG	1 cut(s)	2537
MspR9I	CCNGG	7 cut(s)	245, 618, 619, 1005, 1062, 1820, 2321
Mva1269I	GAATGC	2 cut(s)	1751, 2513
MvaI	CCWGG	2 cut(s)	1820, 2321
MvnI	CGCG	1 cut(s)	265
MwoI	GCNNNNNNNGC	6 cut(s)	36, 346, 622, 654, 723, 2402
NaeI	GCCGGC	1 cut(s)	64
NarI	GGCGCC	1 cut(s)	453
NciI	CCSGG	5 cut(s)	245, 618, 619, 1005, 1062
NcoI	CCATGG	1 cut(s)	2374
NgoMIV	GCCGGC	1 cut(s)	62
NheI	GCTAGC	1 cut(s)	1115
NlaIII	CATG	8 cut(s)	606, 1144, 1537, 1643, 1732, 2011, 2131, 2378
NlaIV	GGNNCC	5 cut(s)	124, 454, 2249, 2325, 2497
NmeAIII	GCCGAG	2 cut(s)	907, 1607
NmuCI	GTSAC	2 cut(s)	481, 2159
NsiI	ATGCAT	2 cut(s)	1676, 1753
OliI	CACNNNNGTG	1 cut(s)	1133
PagI	TCATGA	3 cut(s)	1140, 1533, 1639
PaqCI	CACCTGC	1 cut(s)	642
PciSI	GCTCTTC	3 cut(s)	20, 78, 1506
PcsI	WCGNNNNNNNCGW	2 cut(s)	563, 806
PctI	GAATGC	2 cut(s)	1751, 2513
PdiI	GCCGGC	1 cut(s)	64
PdmI	GAANNNNTTC	1 cut(s)	1499
PfeI	GAWTC	5 cut(s)	229, 832, 1028, 1537, 1643
PfoI	TCCNGGA	1 cut(s)	1818
Ple19I	CGATCG	2 cut(s)	447, 629
PleI	GAGTC	6 cut(s)	164, 322, 416, 805, 1524, 2582
PluTI	GGCGCC	1 cut(s)	456
PmaCI	CACGTG	1 cut(s)	1772
PmlI	CACGTG	1 cut(s)	1772
PpsI	GAGTC	6 cut(s)	164, 322, 416, 805, 1524, 2582
Ppu21I	YACGTR	2 cut(s)	1772, 2524
PpuMI	RGGWCCY	2 cut(s)	122, 2533
PshAI	GACNNNNGTC	1 cut(s)	2358
Psp124BI	GAGCTC	1 cut(s)	2439
Psp1406I	AACGTT	1 cut(s)	1588
Psp5II	RGGWCCY	2 cut(s)	122, 2533
Psp6I	CCWGG	2 cut(s)	1818, 2319
PspCI	CACGTG	1 cut(s)	1772
PspFI	CCCAGC	1 cut(s)	30
PspGI	CCWGG	2 cut(s)	1818, 2319
PspN4I	GGNNCC	5 cut(s)	124, 454, 2249, 2325, 2497
PspPI	GGNCC	8 cut(s)	37, 122, 606, 879, 1107, 1579, 2178, 2533
PspPPI	RGGWCCY	2 cut(s)	122, 2533
PstI	CTGCAG	1 cut(s)	719
PsuI	RGATCY	3 cut(s)	1363, 1448, 1807
PvuI	CGATCG	2 cut(s)	447, 629
PvuII	CAGCTG	2 cut(s)	943, 1634
RsaI	GTAC	6 cut(s)	116, 713, 1019, 1332, 2244, 2522
RsaNI	GTAC	6 cut(s)	115, 712, 1018, 1331, 2243, 2521
RseI	CAYNNNNRTG	2 cut(s)	1133, 2606
SacI	GAGCTC	1 cut(s)	2439
SapI	GCTCTTC	3 cut(s)	20, 78, 1506
SaqAI	TTAA	5 cut(s)	162, 1269, 2286, 2367, 2538
Sau96I	GGNCC	8 cut(s)	37, 122, 606, 879, 1107, 1579, 2178, 2533
ScaI	AGTACT	1 cut(s)	713
SchI	GAGTC	6 cut(s)	164, 322, 416, 806, 1524, 2582
ScrFI	CCNGG	7 cut(s)	245, 618, 619, 1005, 1062, 1820, 2321
SduI	GDGCHC	5 cut(s)	90, 896, 1205, 2280, 2439
SexAI	ACCWGGT	1 cut(s)	2319
SfaNI	GCATC	8 cut(s)	42, 738, 1102, 1301, 1683, 1738, 2082, 2287
SfcI	CTRYAG	2 cut(s)	715, 1890
SfoI	GGCGCC	1 cut(s)	454
SinI	GGWCC	3 cut(s)	122, 1107, 2533
SmaI	CCCGGG	1 cut(s)	619
SmiMI	CAYNNNNRTG	2 cut(s)	1133, 2606
SmlI	CTYRAG	2 cut(s)	1574, 2537
SmoI	CTYRAG	2 cut(s)	1574, 2537
SspDI	GGCGCC	1 cut(s)	452
SspI	AATATT	1 cut(s)	1874
SspMI	CTAG	2 cut(s)	1116, 1458
SstI	GAGCTC	1 cut(s)	2439
StyD4I	CCNGG	7 cut(s)	243, 616, 617, 1003, 1060, 1818, 2319
StyI	CCWWGG	2 cut(s)	2082, 2374
TaaI	ACNGT	2 cut(s)	316, 1853
TaiI	ACGT	4 cut(s)	1197, 1591, 1774, 2526
TatI	WGTACW	1 cut(s)	711
TauI	GCSGC	6 cut(s)	203, 291, 453, 476, 562, 575
TfiI	GAWTC	5 cut(s)	229, 832, 1028, 1537, 1643
Tru1I	TTAA	5 cut(s)	162, 1269, 2286, 2367, 2538
Tru9I	TTAA	5 cut(s)	162, 1269, 2286, 2367, 2538
TscAI	CASTG	3 cut(s)	1205, 1856, 2142
TseFI	GTSAC	2 cut(s)	481, 2159
Tsp45I	GTSAC	2 cut(s)	481, 2159
TspGWI	ACGGA	1 cut(s)	184
TspMI	CCCGGG	1 cut(s)	617
TspRI	CASTG	3 cut(s)	1205, 1856, 2142
Vha464I	CTTAAG	1 cut(s)	2537
VpaK11BI	GGWCC	3 cut(s)	122, 1107, 2533
XagI	CCTNNNNNAGG	2 cut(s)	146, 731
XapI	RAATTY	4 cut(s)	374, 642, 1982, 2426
XcmI	CCANNNNNNNNNTGG	1 cut(s)	583
XmaI	CCCGGG	1 cut(s)	617
XmnI	GAANNNNTTC	1 cut(s)	1499
XspI	CTAG	2 cut(s)	1116, 1458
ZrmI	AGTACT	1 cut(s)	713
Zsp2I	ATGCAT	2 cut(s)	1676, 1753

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.