Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003471

Physical Location & Seq

Genomic Coordinates Reverse (-)

68084071 .. 68088148

Gene Structure 4078 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005494

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGGCGGCTCCGGAACCCGAGGACGAGAAAAGCTGGCAGGAGCTACTCAGAAAGATGCTGCCTTCCGGCGCGCCGTTGCCGGACGACGACCATCTCGACTACTCCATCGCAGTTGAGTACGAAGGCCCTCCGCTGCCCTACGAAGTGCCCAAGGTTGATCCAGTCGACATCGATTCGCTTCACAGCGCTGCTTCCGGAGCTTCGGGTTCGGTTTCGGAGCTTTCTTCGATTCAGGTGGCCGAGCCGGTCGATCGGGAAGCCTCTCGGTTCAGCCGGATCAGGAACGGCGACGCCGCAAACGAAACGCAGACTTCGACGTCGTTGTCGGTAGGTTTCAGCTCTGAATTTTCAGCTCAGGACTCGAACTCGGAGCCGAAACCGGTCGAGGACGACGCCGGAGGAGGAAAGAGAGCGGCGGTCGTGACTTTCAACACACCGAGAGAATCGGAGACGGAAGACGACGGCGACCGGTCCTCGTCGCCGCAATCGGAGCCGGTGGGATCGCCGGTTGCTTGGGCCTCGCCGCTGAAGGTAGCGAGTAAGAGAGGGATTTGCAGCAGGTGTGGAAAAGGGAACAGATTGAAGGAGAGGGAGTGGTGCTTGGTGTGCGACTCAAAGTACTGTAGCAACTGTTTGCTTAAAGCTATGGGATCAATGCCGGAGGGGCGAAAATGTGTGAGCTGCATTGGGCAGCCTATAGATGAGTCCAAGAGGGCTAGTTTGGGCAAGTGTTCAAAGCTTCTTTCTAGGGTTTGTAGTCCTTTGGAGATTGGGCAGATAATGAGGGCCGAGAAGGAGTGTCCGGCCAATCAGCTCCGGCCGCAGCAGTTGGTTGTGAATGGGAGGGAGTTGAGGGAAGAAGAGCTGGCTGAGATTTTGGGATGTGAAGTACCTCCTCAGAAGTTGAAGCCTGGGAGGTACTGGTACGATAAGGATTCGGGCCTCTGGGGAAAGGAGGGAGAGAAGCCTGATTGTATCATTACTCCGAAACTGAATGTTGGGGGTAAGCTTCGGGCTGATGCCAGCAATGGGAACACTAATGTATTCATGAATGGCAGAGAGATCACAAAGATTGAGCTTAGGGTGCTCAAGCTGGCCAAAGTGCAGTGCCCTCCGGATACTCATTTCTGGGTGTATGAAGATGGTACATATGAGGAGGAAGGGCAGAATAACATTAAAGGAAACATATGGGGAAAGGCATCCACCCGTTTCATTTGTTCATTATTCTCATTGCCTGTACCATCTCGTAATCAAAATGGGGCAAGCGAAGATCCTATGACACAACCTTCAAGTAGGTCCATCCCTGAATACTTGGATCAGGCAAGAGTTCAAAAACTTTTGTTATTTGGTCTGGAGGGATCTGGAACTAGCACCATCTTCAAGCAGGCCAAGTTCTTATATGGGAACGAGTTCACTCCCGAGGAGGTGCAGAACATGAAGCTCATGATTCAAAGCAATATGTACAAGTACTTAAGTATATTACTTGAGGGCCGAGAACGTTTTGAAGAGGAAGCTTCATTGCAGAATACTTCTGATTCGTTGGCCACTGACGAGACGCCATCAGTAGTCGATGAAAGTAAGCAATGCATCTATTCTATCAACCAAAGATTCAAGCACTTTTCTGACTGGTTGCTGGATATCATGGCAACCGGAGACTTGGATGCATTCTTCCCTGCTGCTACACGTGAGTATGCTCCTATTGTAGATGAAGTATGGAAAGATTCTGCCATCCAGGAAACATACAAAAGAAGGGAGGAATTACATTGTCTTCCAGAAGTTGCCAAGTATTTCTTAGATCGGGCTATAGAAATATCAAGTAATGAGTATGAGCCAACCGAGAAGGACATTTTATATGCTGAAGGAGTTACTCAAAGCAATGGCCTTACCTCTCTAGAATTTTCATTTGATGATAGAAGCTCCATGTCTGAGCTATACAATGAAAACTATGAATGCCCCCTTCCGTTAACCAAGTATCAACTAATTTGTCTTAGTTCTAAGGGACTAAATGAGGGGTGCAAATGGCTTGCAATGTTTGAAGACGTGAGGGCAGTGATTTTTTGTGTTGCTTTGAGTGACTATGATCAAATGTGGGCCCTTGGTAATGGGCGTCTTCATAATAAATTGCTGGCAAGCAGAGATTTGTTCGAGAGTTTGGTGAGGCACCCTTGTTTTAGTAACACTCCATTTGTTCTTGTGCTGAACAAATATGATGCATTTGAAGACAAGATAGGCCAGGTTCCATTGTCAGCTTGTGAGTGGTTCAATGACTTCAACCCTGTTAAGCCGCACAATAATAGTCAGGCTCTAGCTCAGCAAGCATATTACTATGTGGCAGTGAAATTCAAAGAGCTCTATTTATCCTTAAGCGGTCAAAAGTTATTTGTAGTGCAAACCCGAGCTAGAGATGGTAACTCAGTTGATGTGGCATTCAAGTACATAAGGGAGGTCCTCAAGTGGGATGAAGAAAAGGATGATAATTTCTTTGGAATAAATGGGGATGACTCCTATTTCAGTACAGAAATGAGTTCTTCTCCATATGTCAGGCAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200

Pfam Domains

Protein Families

G-alpha

Protein Analysis

851

Amino Acids

95.38

Weight (kDa)

4.88

Isoelectric Point (pI)

46.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	441 - 816	1.3e-72	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003471)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g17740 FvH4_5g17740
malus_domestica	MD06G1232900.v1.1 MD14G1239900.v1.1
prunus_persica	Prupe.5G237500_v2.0.a1 Prupe.5G237500_v2.0.a1
pyrus_communis	pycom06g20790 pycom14g20150
rosa_chinensis	RchiOBHm_Chr5g0016271 RchiOBHm_Chr7g0177881
rosa_laevigata	RLG00000003463 RLG00000005494 RLG00000019305 RLG00000035269
rosa_multiflora	Rmu_sc0000141.1_g000001 Rmu_sc0004510.1_g000005 Rmu_sc0006214.1_g000003 Rmu_sc0006997.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129380 Rroxscaffold_3G00275470
rosa_rugosa	Rorug04G0425800 Rorug06G0410700 Rorug06G0410800
rosa_samantha	Rh1CG219000 Rh4CG242300 Rh5AG324200 Rh5CG136200 Rh7AG009900 Rh7AG127600 Rh7BG010000 Rh7BG128600 Rh7BG129500 Rh7CG130400 Rh7CG131800 Rh7DG010000
rosa_wichuraiana	Rw7G000830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	549
AasI	GACNNNNNNGTC	2 cut(s)	316, 322
AatII	GACGTC	1 cut(s)	320
Acc36I	ACCTGC	1 cut(s)	549
AccB1I	GGYRCC	1 cut(s)	2160
AccBSI	CCGCTC	1 cut(s)	413
AccI	GTMKAC	1 cut(s)	165
AccII	CGCG	1 cut(s)	71
AccIII	TCCGGA	3 cut(s)	10, 194, 1114
AclI	AACGTT	1 cut(s)	1498
AclWI	GGATC	7 cut(s)	152, 284, 508, 658, 1265, 1323, 1366
AcoI	YGGCCR	5 cut(s)	237, 804, 818, 1095, 1542
AcsI	RAATTY	3 cut(s)	344, 1895, 2339
AcuI	CTGAAG	2 cut(s)	548, 1878
AcvI	CACGTG	1 cut(s)	1685
AcyI	GRCGYC	5 cut(s)	291, 317, 393, 1556, 2107
AfeI	AGCGCT	1 cut(s)	187
AfiI	CCNNNNNNNGG	2 cut(s)	1029, 2455
AflII	CTTAAG	2 cut(s)	1471, 2362
AflIII	ACRYGT	1 cut(s)	1682
AgeI	ACCGGT	2 cut(s)	379, 468
AjiI	CACGTC	1 cut(s)	2041
AjnI	CCWGG	3 cut(s)	910, 1731, 2232
Alw21I	GWGCWC	2 cut(s)	1089, 2352
Alw26I	GTCTC	3 cut(s)	443, 1547, 1647
AlwI	GGATC	7 cut(s)	152, 284, 508, 658, 1265, 1323, 1366
Ama87I	CYCGRG	3 cut(s)	17, 1418, 2394
Aor13HI	TCCGGA	3 cut(s)	10, 194, 1114
Aor51HI	AGCGCT	1 cut(s)	187
ApaI	GGGCCC	1 cut(s)	2095
ApeKI	GCWGC	8 cut(s)	58, 133, 188, 555, 681, 691, 823, 1676
ApoI	RAATTY	3 cut(s)	344, 1895, 2339
AscI	GGCGCGCC	1 cut(s)	69
AsiGI	ACCGGT	2 cut(s)	379, 468
Asp700I	GAANNNNTTC	1 cut(s)	1409
AspLEI	GCGC	3 cut(s)	71, 73, 188
AsuHPI	GGTGA	1 cut(s)	2167
AvaI	CYCGRG	3 cut(s)	17, 1418, 2394
AvaII	GGWCC	3 cut(s)	471, 1296, 2446
BaeGI	GKGCMC	3 cut(s)	150, 1112, 2095
BalI	TGGCCA	2 cut(s)	1097, 1544
BanI	GGYRCC	1 cut(s)	2160
BanII	GRGCYC	2 cut(s)	2095, 2352
BbrPI	CACGTG	1 cut(s)	1685
BbsI	GAAGAC	5 cut(s)	462, 1760, 2043, 2102, 2226
Bbv12I	GWGCWC	2 cut(s)	1089, 2352
BbvI	GCAGC	8 cut(s)	45, 120, 175, 567, 668, 703, 835, 1663
BccI	CCATC	9 cut(s)	99, 113, 1136, 1249, 1307, 1382, 1567, 1736, 2399
BceAI	ACGGC	3 cut(s)	58, 301, 478
BciT130I	CCWGG	3 cut(s)	912, 1733, 2234
BciVI	GTATCC	1 cut(s)	1111
BclI	TGATCA	1 cut(s)	2080
BcoDI	GTCTC	3 cut(s)	443, 1547, 1647
BfaI	CTAG	6 cut(s)	717, 747, 1368, 1892, 2306, 2400
BfmI	CTRYAG	3 cut(s)	622, 696, 1803
BfoI	RGCGCY	1 cut(s)	189
BfrI	CTTAAG	2 cut(s)	1471, 2362
BfuAI	ACCTGC	1 cut(s)	549
BfuI	GTATCC	1 cut(s)	1111
BglI	GCCNNNNNGGC	1 cut(s)	664
BlpI	GCTNAGC	1 cut(s)	2310
BmcAI	AGTACT	2 cut(s)	620, 1469
Bme1390I	CCNGG	3 cut(s)	912, 1733, 2234
Bme18I	GGWCC	3 cut(s)	471, 1296, 2446
BmeT110I	CYCGRG	3 cut(s)	17, 1418, 2394
BmgBI	CACGTC	1 cut(s)	2041
BmiI	GGNNCC	7 cut(s)	9, 15, 372, 492, 2093, 2162, 2238
BmrFI	CCNGG	3 cut(s)	912, 1733, 2234
BmsI	GCATC	6 cut(s)	45, 1009, 1208, 1596, 1651, 2200
BpiI	GAAGAC	5 cut(s)	462, 1760, 2043, 2102, 2226
BplI	GAGNNNNNCTC	2 cut(s)	2515, 2547
BpmI	CTGGAG	1 cut(s)	1373
Bpu10I	CCTNAGC	2 cut(s)	354, 1079
Bpu1102I	GCTNAGC	1 cut(s)	2310
BpuEI	CTTGAG	3 cut(s)	1073, 1505, 2435
Bsa29I	ATCGAT	1 cut(s)	171
BsaAI	YACGTR	1 cut(s)	1685
BsaHI	GRCGYC	5 cut(s)	291, 317, 393, 1556, 2107
BsaJI	CCNNGG	5 cut(s)	18, 150, 911, 1419, 2095
BsaWI	WCCGGW	6 cut(s)	10, 194, 379, 468, 1114, 1649
BsaXI	ACNNNNNCTCC	2 cut(s)	578, 608
Bsc4I	CCNNNNNNNGG	2 cut(s)	1029, 2455
Bse118I	RCCGGY	5 cut(s)	244, 379, 468, 493, 505
Bse1I	ACTGG	3 cut(s)	161, 926, 1631
Bse3DI	GCAATG	6 cut(s)	1033, 1229, 1517, 1589, 1882, 2034
BseAI	TCCGGA	3 cut(s)	10, 194, 1114
BseBI	CCWGG	3 cut(s)	912, 1733, 2234
BseCI	ATCGAT	1 cut(s)	171
BseDI	CCNNGG	5 cut(s)	18, 150, 911, 1419, 2095
BseGI	GGATG	8 cut(s)	887, 1199, 1299, 1666, 1728, 2464, 2476, 2503
BseLI	CCNNNNNNNGG	2 cut(s)	1029, 2455
BseMI	GCAATG	6 cut(s)	1033, 1229, 1517, 1589, 1882, 2034
BseMII	CTCAG	7 cut(s)	61, 368, 861, 911, 1917, 2324, 2427
BseNI	ACTGG	3 cut(s)	161, 926, 1631
BsePI	GCGCGC	1 cut(s)	69
BseRI	GAGGAG	4 cut(s)	414, 885, 1169, 1436
BseSI	GKGCMC	3 cut(s)	150, 1112, 2095
BseX3I	CGGCCG	1 cut(s)	818
BseXI	GCAGC	8 cut(s)	45, 120, 175, 567, 668, 703, 835, 1663
BsgI	GTGCAG	2 cut(s)	1124, 1448
Bsh1236I	CGCG	1 cut(s)	71
Bsh1285I	CGRYCG	6 cut(s)	249, 253, 384, 420, 469, 821
BshNI	GGYRCC	1 cut(s)	2160
BshTI	ACCGGT	2 cut(s)	379, 468
BshVI	ATCGAT	1 cut(s)	171
BsiEI	CGRYCG	6 cut(s)	249, 253, 384, 420, 469, 821
BsiHKAI	GWGCWC	2 cut(s)	1089, 2352
BsiHKCI	CYCGRG	3 cut(s)	17, 1418, 2394
BslFI	GGGAC	1 cut(s)	2013
BslI	CCNNNNNNNGG	2 cut(s)	1029, 2455
BsmAI	GTCTC	3 cut(s)	443, 1547, 1647
BsmBI	CGTCTC	2 cut(s)	443, 1547
BsmFI	GGGAC	1 cut(s)	2013
BsmI	GAATGC	3 cut(s)	1664, 1955, 2426
BsoBI	CYCGRG	3 cut(s)	17, 1418, 2394
Bsp120I	GGGCCC	1 cut(s)	2091
Bsp1286I	GDGCHC	5 cut(s)	150, 1089, 1112, 2095, 2352
Bsp13I	TCCGGA	3 cut(s)	10, 194, 1114
Bsp1407I	TGTACA	1 cut(s)	1461
Bsp1720I	GCTNAGC	1 cut(s)	2310
BspCNI	CTCAG	7 cut(s)	60, 367, 862, 910, 1918, 2323, 2426
BspDI	ATCGAT	1 cut(s)	171
BspEI	TCCGGA	3 cut(s)	10, 194, 1114
BspFNI	CGCG	1 cut(s)	71
BspHI	TCATGA	2 cut(s)	1047, 1443
BspLI	GGNNCC	7 cut(s)	9, 15, 372, 492, 2093, 2162, 2238
BspMI	ACCTGC	1 cut(s)	549
BspPI	GGATC	7 cut(s)	152, 284, 508, 658, 1265, 1323, 1366
BspQI	GCTCTTC	1 cut(s)	855
BspT107I	GGYRCC	1 cut(s)	2160
BspTI	CTTAAG	2 cut(s)	1471, 2362
BsrBI	CCGCTC	1 cut(s)	413
BsrDI	GCAATG	6 cut(s)	1033, 1229, 1517, 1589, 1882, 2034
BsrFI	RCCGGY	5 cut(s)	244, 379, 468, 493, 505
BsrGI	TGTACA	1 cut(s)	1461
BsrI	ACTGG	3 cut(s)	161, 926, 1631
BssAI	RCCGGY	5 cut(s)	244, 379, 468, 493, 505
BssECI	CCNNGG	5 cut(s)	18, 150, 911, 1419, 2095
BssHII	GCGCGC	1 cut(s)	69
BssNI	GRCGYC	5 cut(s)	291, 317, 393, 1556, 2107
BssT1I	CCWWGG	2 cut(s)	150, 2095
Bst2UI	CCWGG	3 cut(s)	912, 1733, 2234
Bst4CI	ACNGT	2 cut(s)	623, 632
Bst6I	CTCTTC	2 cut(s)	855, 1500
BstACI	GRCGYC	5 cut(s)	291, 317, 393, 1556, 2107
BstAFI	CTTAAG	2 cut(s)	1471, 2362
BstAUI	TGTACA	1 cut(s)	1461
BstBAI	YACGTR	1 cut(s)	1685
BstF5I	GGATG	8 cut(s)	887, 1199, 1299, 1666, 1728, 2464, 2476, 2503
BstFNI	CGCG	1 cut(s)	71
BstH2I	RGCGCY	1 cut(s)	189
BstHHI	GCGC	3 cut(s)	71, 73, 188
BstMAI	GTCTC	3 cut(s)	443, 1547, 1647
BstMCI	CGRYCG	6 cut(s)	249, 253, 384, 420, 469, 821
BstMWI	GCNNNNNNNGC	7 cut(s)	197, 490, 606, 664, 820, 1084, 2315
BstNI	CCWGG	3 cut(s)	912, 1733, 2234
BstSCI	CCNGG	3 cut(s)	910, 1731, 2232
BstSFI	CTRYAG	3 cut(s)	622, 696, 1803
BstSLI	GKGCMC	3 cut(s)	150, 1112, 2095
BstUI	CGCG	1 cut(s)	71
BstV1I	GCAGC	8 cut(s)	45, 120, 175, 567, 668, 703, 835, 1663
BstV2I	GAAGAC	5 cut(s)	462, 1760, 2043, 2102, 2226
BstX2I	RGATCY	2 cut(s)	1270, 1358
BstYI	RGATCY	2 cut(s)	1270, 1358
BstZI	CGGCCG	1 cut(s)	818
Bsu15I	ATCGAT	1 cut(s)	171
BsuI	GTATCC	1 cut(s)	1111
BsuTUI	ATCGAT	1 cut(s)	171
BtgZI	GCGATG	1 cut(s)	91
BtrI	CACGTC	1 cut(s)	2041
BtsCI	GGATG	8 cut(s)	887, 1199, 1299, 1666, 1728, 2464, 2476, 2503
BtsI	GCAGTG	3 cut(s)	1112, 2055, 2340
BtsIMutI	CAGTG	4 cut(s)	1112, 1545, 2055, 2340
BveI	ACCTGC	1 cut(s)	549
CciI	TCATGA	2 cut(s)	1047, 1443
CfoI	GCGC	3 cut(s)	71, 73, 188
Cfr10I	RCCGGY	5 cut(s)	244, 379, 468, 493, 505
ClaI	ATCGAT	1 cut(s)	171
CseI	GACGC	4 cut(s)	299, 401, 1564, 2096
CspAI	ACCGGT	2 cut(s)	379, 468
CviAII	CATG	5 cut(s)	1048, 1435, 1444, 1642, 1921
DrdI	GACNNNNNNGTC	2 cut(s)	316, 322
DseDI	GACNNNNNNGTC	2 cut(s)	316, 322
EaeI	YGGCCR	5 cut(s)	237, 804, 818, 1095, 1542
EagI	CGGCCG	1 cut(s)	818
Eam1104I	CTCTTC	2 cut(s)	855, 1500
EarI	CTCTTC	2 cut(s)	855, 1500
Ecl136II	GAGCTC	1 cut(s)	2350
EclXI	CGGCCG	1 cut(s)	818
Eco130I	CCWWGG	2 cut(s)	150, 2095
Eco24I	GRGCYC	2 cut(s)	2095, 2352
Eco32I	GATATC	1 cut(s)	1639
Eco47I	GGWCC	3 cut(s)	471, 1296, 2446
Eco47III	AGCGCT	1 cut(s)	187
Eco52I	CGGCCG	1 cut(s)	818
Eco53kI	GAGCTC	1 cut(s)	2350
Eco57I	CTGAAG	2 cut(s)	548, 1878
Eco72I	CACGTG	1 cut(s)	1685
Eco88I	CYCGRG	3 cut(s)	17, 1418, 2394
EcoICRI	GAGCTC	1 cut(s)	2350
EcoO109I	RGGNCCY	3 cut(s)	125, 2092, 2446
EcoRII	CCWGG	3 cut(s)	910, 1731, 2232
EcoRV	GATATC	1 cut(s)	1639
EcoT14I	CCWWGG	2 cut(s)	150, 2095
EcoT22I	ATGCAT	3 cut(s)	1589, 1666, 2215
EcoT38I	GRGCYC	2 cut(s)	2095, 2352
ErhI	CCWWGG	2 cut(s)	150, 2095
Esp3I	CGTCTC	2 cut(s)	443, 1547
FaeI	CATG	5 cut(s)	1051, 1438, 1447, 1645, 1924
FalI	AAGNNNNNCTT	2 cut(s)	1775, 1807
FaqI	GGGAC	1 cut(s)	2013
FatI	CATG	5 cut(s)	1047, 1434, 1443, 1641, 1920
FauNDI	CATATG	3 cut(s)	1150, 1187, 2536
FbaI	TGATCA	1 cut(s)	2080
FblI	GTMKAC	1 cut(s)	165
FokI	GGATG	8 cut(s)	894, 1186, 1286, 1673, 1715, 2471, 2483, 2510
FriOI	GRGCYC	2 cut(s)	2095, 2352
FspBI	CTAG	6 cut(s)	717, 747, 1368, 1892, 2306, 2400
GlaI	GCGC	3 cut(s)	70, 72, 187
GsuI	CTGGAG	1 cut(s)	1373
HaeII	RGCGCY	1 cut(s)	189
HgaI	GACGC	4 cut(s)	299, 401, 1564, 2096
HhaI	GCGC	3 cut(s)	71, 73, 188
Hin1I	GRCGYC	5 cut(s)	291, 317, 393, 1556, 2107
Hin1II	CATG	5 cut(s)	1051, 1438, 1447, 1645, 1924
Hin6I	GCGC	3 cut(s)	69, 71, 186
HinP1I	GCGC	3 cut(s)	69, 71, 186
HincII	GTYRAC	2 cut(s)	166, 1965
HindII	GTYRAC	2 cut(s)	166, 1965
HindIII	AAGCTT	3 cut(s)	737, 1007, 1512
HpaI	GTTAAC	1 cut(s)	1965
HphI	GGTGA	1 cut(s)	2167
Hpy166II	GTNNAC	3 cut(s)	166, 1413, 1965
Hpy8I	GTNNAC	3 cut(s)	166, 1413, 1965
Hpy99I	CGWCG	7 cut(s)	89, 293, 319, 322, 395, 464, 481
HpyCH4III	ACNGT	2 cut(s)	623, 632
HpyCH4IV	ACGT	4 cut(s)	317, 1498, 1684, 2040
HpyF10VI	GCNNNNNNNGC	7 cut(s)	197, 490, 606, 664, 820, 1084, 2315
HpySE526I	ACGT	4 cut(s)	317, 1498, 1684, 2040
Hsp92I	GRCGYC	5 cut(s)	291, 317, 393, 1556, 2107
Hsp92II	CATG	5 cut(s)	1051, 1438, 1447, 1645, 1924
HspAI	GCGC	3 cut(s)	69, 71, 186
Kpn2I	TCCGGA	3 cut(s)	10, 194, 1114
Ksp22I	TGATCA	1 cut(s)	2080
KspAI	GTTAAC	1 cut(s)	1965
LguI	GCTCTTC	1 cut(s)	855
LmnI	GCTCC	9 cut(s)	13, 40, 197, 217, 370, 490, 819, 1699, 1922
Lsp1109I	GCAGC	8 cut(s)	45, 120, 175, 567, 668, 703, 835, 1663
LweI	GCATC	6 cut(s)	45, 1009, 1208, 1596, 1651, 2200
MaeI	CTAG	6 cut(s)	717, 747, 1368, 1892, 2306, 2400
MaeII	ACGT	4 cut(s)	317, 1498, 1684, 2040
MaeIII	GTNAC	5 cut(s)	421, 1864, 2072, 2174, 2408
MbiI	CCGCTC	1 cut(s)	413
MflI	RGATCY	2 cut(s)	1270, 1358
MhlI	GDGCHC	5 cut(s)	150, 1089, 1112, 2095, 2352
MlsI	TGGCCA	2 cut(s)	1097, 1544
MluCI	AATT	7 cut(s)	344, 1757, 1895, 1980, 2120, 2339, 2476
MluNI	TGGCCA	2 cut(s)	1097, 1544
MlyI	GAGTC	4 cut(s)	353, 605, 713, 2495
Mox20I	TGGCCA	2 cut(s)	1097, 1544
Mph1103I	ATGCAT	3 cut(s)	1589, 1666, 2215
MroI	TCCGGA	3 cut(s)	10, 194, 1114
MroXI	GAANNNNTTC	1 cut(s)	1409
MscI	TGGCCA	2 cut(s)	1097, 1544
MseI	TTAA	6 cut(s)	639, 1176, 1472, 1964, 2280, 2363
Msp20I	TGGCCA	2 cut(s)	1097, 1544
MspA1I	CMGCKG	2 cut(s)	133, 526
MspCI	CTTAAG	2 cut(s)	1471, 2362
MspR9I	CCNGG	3 cut(s)	912, 1733, 2234
Mva1269I	GAATGC	3 cut(s)	1664, 1955, 2426
MvaI	CCWGG	3 cut(s)	912, 1733, 2234
MvnI	CGCG	1 cut(s)	71
MwoI	GCNNNNNNNGC	7 cut(s)	197, 490, 606, 664, 820, 1084, 2315
NdeI	CATATG	3 cut(s)	1150, 1187, 2536
NlaIII	CATG	5 cut(s)	1051, 1438, 1447, 1645, 1924
NlaIV	GGNNCC	7 cut(s)	9, 15, 372, 492, 2093, 2162, 2238
NmeAIII	GCCGAG	3 cut(s)	265, 814, 1517
NmuCI	GTSAC	2 cut(s)	421, 2072
NsiI	ATGCAT	3 cut(s)	1589, 1666, 2215
PagI	TCATGA	2 cut(s)	1047, 1443
PalAI	GGCGCGCC	1 cut(s)	69
PaqCI	CACCTGC	1 cut(s)	549
PauI	GCGCGC	1 cut(s)	69
PciSI	GCTCTTC	1 cut(s)	855
PcsI	WCGNNNNNNNCGW	3 cut(s)	93, 297, 311
PctI	GAATGC	3 cut(s)	1664, 1955, 2426
PdmI	GAANNNNTTC	1 cut(s)	1409
PfeI	GAWTC	8 cut(s)	173, 229, 443, 935, 1447, 1535, 1608, 1721
PfoI	TCCNGGA	1 cut(s)	1731
PinAI	ACCGGT	2 cut(s)	379, 468
Ple19I	CGATCG	1 cut(s)	253
PleI	GAGTC	4 cut(s)	353, 605, 712, 2495
PmaCI	CACGTG	1 cut(s)	1685
PmlI	CACGTG	1 cut(s)	1685
PpsI	GAGTC	4 cut(s)	353, 605, 712, 2495
Ppu21I	YACGTR	1 cut(s)	1685
PpuMI	RGGWCCY	1 cut(s)	2446
Psp124BI	GAGCTC	1 cut(s)	2352
Psp1406I	AACGTT	1 cut(s)	1498
Psp5II	RGGWCCY	1 cut(s)	2446
Psp6I	CCWGG	3 cut(s)	910, 1731, 2232
PspCI	CACGTG	1 cut(s)	1685
PspGI	CCWGG	3 cut(s)	910, 1731, 2232
PspN4I	GGNNCC	7 cut(s)	9, 15, 372, 492, 2093, 2162, 2238
PspOMI	GGGCCC	1 cut(s)	2091
PspPPI	RGGWCCY	1 cut(s)	2446
PsuI	RGATCY	2 cut(s)	1270, 1358
PteI	GCGCGC	1 cut(s)	69
PvuI	CGATCG	1 cut(s)	253
SacI	GAGCTC	1 cut(s)	2352
SalI	GTCGAC	1 cut(s)	164
SapI	GCTCTTC	1 cut(s)	855
SaqAI	TTAA	6 cut(s)	639, 1176, 1472, 1964, 2280, 2363
ScaI	AGTACT	2 cut(s)	620, 1469
SchI	GAGTC	4 cut(s)	353, 605, 713, 2495
ScrFI	CCNGG	3 cut(s)	912, 1733, 2234
SduI	GDGCHC	5 cut(s)	150, 1089, 1112, 2095, 2352
SfaNI	GCATC	6 cut(s)	45, 1009, 1208, 1596, 1651, 2200
SfcI	CTRYAG	3 cut(s)	622, 696, 1803
SgsI	GGCGCGCC	1 cut(s)	69
SinI	GGWCC	3 cut(s)	471, 1296, 2446
SmlI	CTYRAG	5 cut(s)	1088, 1471, 1484, 2362, 2450
SmoI	CTYRAG	5 cut(s)	1088, 1471, 1484, 2362, 2450
Sse9I	AATT	7 cut(s)	344, 1757, 1895, 1980, 2120, 2339, 2476
SspMI	CTAG	6 cut(s)	717, 747, 1368, 1892, 2306, 2400
SstI	GAGCTC	1 cut(s)	2352
StyD4I	CCNGG	3 cut(s)	910, 1731, 2232
StyI	CCWWGG	2 cut(s)	150, 2095
TaaI	ACNGT	2 cut(s)	623, 632
TaiI	ACGT	4 cut(s)	320, 1501, 1687, 2043
TasI	AATT	7 cut(s)	344, 1757, 1895, 1980, 2120, 2339, 2476
TatI	WGTACW	5 cut(s)	618, 1461, 1467, 2433, 2513
TauI	GCSGC	7 cut(s)	8, 296, 416, 484, 526, 823, 2287
TfiI	GAWTC	8 cut(s)	173, 229, 443, 935, 1447, 1535, 1608, 1721
Tru1I	TTAA	6 cut(s)	639, 1176, 1472, 1964, 2280, 2363
Tru9I	TTAA	6 cut(s)	639, 1176, 1472, 1964, 2280, 2363
TscAI	CASTG	4 cut(s)	1112, 1552, 2055, 2340
TseFI	GTSAC	2 cut(s)	421, 2072
TseI	GCWGC	8 cut(s)	58, 133, 188, 555, 681, 691, 823, 1676
Tsp45I	GTSAC	2 cut(s)	421, 2072
TspGWI	ACGGA	2 cut(s)	467, 1950
TspRI	CASTG	4 cut(s)	1112, 1552, 2055, 2340
Vha464I	CTTAAG	2 cut(s)	1471, 2362
VpaK11BI	GGWCC	3 cut(s)	471, 1296, 2446
XapI	RAATTY	3 cut(s)	344, 1895, 2339
XbaI	TCTAGA	1 cut(s)	1891
XmiI	GTMKAC	1 cut(s)	165
XmnI	GAANNNNTTC	1 cut(s)	1409
XspI	CTAG	6 cut(s)	717, 747, 1368, 1892, 2306, 2400
ZraI	GACGTC	1 cut(s)	318
ZrmI	AGTACT	2 cut(s)	620, 1469
Zsp2I	ATGCAT	3 cut(s)	1589, 1666, 2215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.