Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003471

Physical Location & Seq

Genomic Coordinates Reverse (-)

31889712 .. 31895325

Gene Structure 5614 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1239900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGGCTTCCGAACCGGAGGGCGGGAAGAGCTGGGAGGACCTCCTCAGAAAGATGCTGCCTGCCGGCGCACCATTACCCGACGAAGAGCACCTCGACTACTCCATCGCCGTCGAATACGAAGGCTCTCCTCTGCACTACGACCCGCCCAAGGTTGATCCGGTTAACCTCGACTCTCTCGATCTTTACTCTTCTTCCGTGACCGCTGGTTCGGTTGAAGCCCCGTCTTCGATTCCGGTGGCGTCGCCGGTCGGTCAGAAGGTCTCGCGGTTCAGTCGGGTGAGTAACGGCGGCGTTGTGAAGGAGACGAGTAGAACGACGTCGTCTTCGACTCCGCGGACGCAACCGGAGCTGCGAAACCGCAGGGAAGGATCGGAATTTGAAGGAGCAGAGGAAGGTTTCAGCTCTGAGTTGCCGGCTCAAGACTCGAACTTGGAGCACAAACCGACCGGCGGCGCCACCGGAGGAAAGAGGGCGGCTGTCGTCACTTTCAATGAACCAAGAGATTCGGAGAACGAGGACGAGGGTGAAGATAACGACTCGTCCCCGCAATCGAGCGCGGCCACCGAACAGGTGGGATCGCCGGTTGTAGGGGCCTCTTCGCCCGGGAGGCGATCGAGTAAGAGAGGGATTTGCAGCAGGTGCGGGAAAGGGAACAGATTGAAGGAGAGAGAGTGGTGTTTGGTGTGCGACGCCAAGTACTGCAGCAATTGCTTGCTGAAGGCGATGGGGTCGATGCCGGAGGGGAGGAAATGTGTGAGCTGCATTGGGCAGCCAATAGACGAGTCCAAGAGGTCGAACTTGGGGAAGTGTTCGAGGATCCTGTCGAGGGTTTGCAGCCCTTTGGAGATTCGGCAGATAATGAGGGGCGAGAAGGAGTGCCCCGCCAATCAGCTTCGGCCGGAGCAGTTGGTTGTGAATGGAAGCAAGCTGAGGGAAGAAGAACTGGCTGAGATTTTGGGATGTGAATTGCCTCCTCAGAAGTTGAAACCTGGGAGGTATTGGTACGATAAGGATTCCGGTCTTTGGGGAAAGGAGGGTGAGAAGCCGGATAGGATTATTAGCTCGAAACTCAATGTTGGGGGTAAGATTCGGTTTGATGCAAGCAATGGGAACACCAAAGTGTTCATGAATGGCCGAGAGATCACAAAGATTGAGCGTAGGGTGTTGAAGCTGGCCAAAGTGCAGTGCCCTCCGGATACTCATTTCTGGGTGTATGATGATGGTTCTTATGAGGAGGAAGGCCAGAATAACATTAAAGGGAATATATGGGGAAAGGCATCCACTCGTTTCATTTGTTCATTATTCTCATTGCCTGTACCACCTGGTAATCAAAATGGGGCACAAGAAGATCCGATGACACCTTCAAGCAGGTCAGTCCGGGAATTCTGGGAACAGGGAAGAGTTCAGAAACTTCTGTTATTTGGCTTGGAGGGATCTGGAACTAGCACCATCTTCAAGCAGGCTAAGCTCTTATACGGGAACAAGTTCACTTCCGAAGAGCTGCAGAACATCAAACTCATGATTCAGAGCAATATGTATAAGTATCTAAGTATATTACTTGAGGGCCGAGAACGTTTTGAAGAGGAAGCATTGACAGAGAATAGGATATCTTTGTTGGCTGCTCATGAGTCCCCTTCAGTTGATGAAAGTGAGCAATGCATCTATGCAATCAACCAAAGGTTCAAGCACTTTTCTGACTGGTTACTGGATATCATGGCAACTGGAGACTTGGATGCATTCTTTCCTGCTGCGACACGTGAGTATGCTCCTATTGTGGATGAAGTGTGGAGAGATTCTGCCATCCAGGAAACATACAAAAGGAGGGAGGAACTAGACTGCCTTCCTGAAGTTGCCAAATATTTCTTAGATCGGGCTATAGAAATATCAAGTAACGAGTATGAGCCGTCCGAGAAAGACATTTTATATGCTGAGGGCGTTACACAGAGCAATGGGCTTGCCTCTATGGAATTTTCATTTGATGACAGAAGTGCCACGTCTGAGCCATACAATGAAAACTATGAATGTTCCCTTCCCTTGACTAAGTATCAACTAATTCGCATTAATTCCAAGGGACTGAGTGATGGTTGCAAATGGCTGGAAATGTTTGCAGATGTGAGGGCAGTGATTTTTTGTGTTGTGTTGAGCGACTATGATCAAATGTGGGCCCACGGTAATGGTCTTCTTCGTAATAAATTGCTGGCAAGCAGAGATTTGTTTGAGAGTTTGGTACGGCACCCTTGTTTCAGAGACACTCCGTTTGTGCTCTTTCTGAACAAATACGATGCCTTTGAAGGCAAGATAAACCAGGTTCCGTTGTCTGCCTGCGAGTGGCTAAAGGACTTCAGACCTGTTAGGCTTCATGGTAATAGTCAGTCTCTGGCACAGCAAGCATATTATTACGTGGCGGTGAAATTCAAAGAGCTGTATTCATCCATAAGCGGCGAAAAGTTGTTTGTGGCGCAGACCCGAGCTCGGGAGGCTGCCTCAGTTGACGAGGCATTCAAGTACGTACGGGAGGTCCTCAAGTGGGATGAAGAAAAGAATGATAATTTCTTTGCAGTAAATGGGGACGACTCATTTTACAGCACGGAGATGAGTTCTTCTCCATATGTCAGGGAGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200

Pfam Domains

Protein Families

G-alpha

Protein Analysis

875

Amino Acids

98.18

Weight (kDa)

5.18

Isoelectric Point (pI)

53.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	468 - 840	1.5e-69	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003471)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g17740 FvH4_5g17740
malus_domestica	MD06G1232900.v1.1 MD14G1239900.v1.1
prunus_persica	Prupe.5G237500_v2.0.a1 Prupe.5G237500_v2.0.a1
pyrus_communis	pycom06g20790 pycom14g20150
rosa_chinensis	RchiOBHm_Chr5g0016271 RchiOBHm_Chr7g0177881
rosa_laevigata	RLG00000003463 RLG00000005494 RLG00000019305 RLG00000035269
rosa_multiflora	Rmu_sc0000141.1_g000001 Rmu_sc0004510.1_g000005 Rmu_sc0006214.1_g000003 Rmu_sc0006997.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129380 Rroxscaffold_3G00275470
rosa_rugosa	Rorug04G0425800 Rorug06G0410700 Rorug06G0410800
rosa_samantha	Rh1CG219000 Rh4CG242300 Rh5AG324200 Rh5CG136200 Rh7AG009900 Rh7AG127600 Rh7BG010000 Rh7BG128600 Rh7BG129500 Rh7CG130400 Rh7CG131800 Rh7DG010000
rosa_wichuraiana	Rw7G000830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	627
AatII	GACGTC	1 cut(s)	320
Acc36I	ACCTGC	2 cut(s)	627, 1359
AccB1I	GGYRCC	2 cut(s)	452, 2232
AccII	CGCG	3 cut(s)	265, 334, 557
AccIII	TCCGGA	1 cut(s)	1192
AclI	AACGTT	1 cut(s)	1573
AclWI	GGATC	7 cut(s)	149, 376, 583, 811, 824, 1343, 1441
AcoI	YGGCCR	4 cut(s)	558, 896, 1132, 1173
AcsI	RAATTY	4 cut(s)	374, 1382, 1967, 2411
AcuI	CTGAAG	4 cut(s)	737, 1620, 1866, 2326
AcvI	CACGTG	1 cut(s)	1757
AcyI	GRCGYC	4 cut(s)	239, 317, 453, 690
AfaI	GTAC	6 cut(s)	698, 1004, 1317, 2229, 2507, 2511
AflIII	ACRYGT	1 cut(s)	1754
AjiI	CACGTC	1 cut(s)	1995
AjnI	CCWGG	4 cut(s)	988, 1321, 1803, 2304
AleI	CACNNNNGTG	1 cut(s)	1118
Alw21I	GWGCWC	4 cut(s)	90, 438, 2265, 2473
Alw26I	GTCTC	5 cut(s)	265, 296, 1719, 2241, 2379
AlwI	GGATC	7 cut(s)	149, 376, 583, 811, 824, 1343, 1441
AlwNI	CAGNNNCTG	1 cut(s)	2377
Ama87I	CYCGRG	3 cut(s)	602, 2466, 2472
Aor13HI	TCCGGA	1 cut(s)	1192
AoxI	GGCC	8 cut(s)	558, 591, 896, 1132, 1173, 1240, 1564, 2163
ApaI	GGGCCC	1 cut(s)	2167
ApoI	RAATTY	4 cut(s)	374, 1382, 1967, 2411
ArsI	GACNNNNNNTTYG	6 cut(s)	190, 222, 307, 339, 2240, 2272
AseI	ATTAAT	1 cut(s)	2061
Asp700I	GAANNNNTTC	1 cut(s)	1484
AspLEI	GCGC	4 cut(s)	68, 455, 557, 2461
AspS9I	GGNCC	6 cut(s)	37, 591, 1564, 2163, 2164, 2518
AsuC2I	CCSGG	3 cut(s)	603, 604, 1379
AsuHPI	GGTGA	4 cut(s)	289, 536, 1049, 2419
AvaI	CYCGRG	3 cut(s)	602, 2466, 2472
AvaII	GGWCC	2 cut(s)	37, 2518
BaeGI	GKGCMC	4 cut(s)	881, 1190, 1342, 2167
BalI	TGGCCA	1 cut(s)	1175
BamHI	GGATCC	1 cut(s)	816
BanI	GGYRCC	2 cut(s)	452, 2232
BanII	GRGCYC	2 cut(s)	2167, 2473
BbrPI	CACGTG	1 cut(s)	1757
BbsI	GAAGAC	3 cut(s)	216, 315, 2171
Bbv12I	GWGCWC	4 cut(s)	90, 438, 2265, 2473
BbvCI	CCTCAGC	2 cut(s)	929, 1929
BccI	CCATC	6 cut(s)	110, 718, 1214, 1457, 1808, 2075
BceAI	ACGGC	4 cut(s)	92, 301, 1888, 2246
BciT130I	CCWGG	4 cut(s)	990, 1323, 1805, 2306
BciVI	GTATCC	1 cut(s)	1189
BclI	TGATCA	1 cut(s)	2152
BcnI	CCSGG	3 cut(s)	603, 604, 1379
BcoDI	GTCTC	5 cut(s)	265, 296, 1719, 2241, 2379
BfaI	CTAG	2 cut(s)	1443, 1832
BfmI	CTRYAG	3 cut(s)	700, 1502, 1875
BfoI	RGCGCY	1 cut(s)	456
BfuAI	ACCTGC	2 cut(s)	627, 1359
BfuI	GTATCC	1 cut(s)	1189
BglI	GCCNNNNNGGC	1 cut(s)	607
BlpI	GCTNAGC	1 cut(s)	1464
BmcAI	AGTACT	1 cut(s)	698
Bme1390I	CCNGG	7 cut(s)	603, 604, 990, 1323, 1379, 1805, 2306
Bme18I	GGWCC	2 cut(s)	37, 2518
BmeT110I	CYCGRG	3 cut(s)	602, 2466, 2472
BmgBI	CACGTC	1 cut(s)	1995
BmgT120I	GGNCC	6 cut(s)	37, 591, 1564, 2163, 2164, 2518
BmiI	GGNNCC	6 cut(s)	454, 592, 818, 2165, 2234, 2310
BmrFI	CCNGG	7 cut(s)	603, 604, 990, 1323, 1379, 1805, 2306
BmsI	GCATC	7 cut(s)	42, 723, 1087, 1286, 1668, 1723, 2272
BpiI	GAAGAC	3 cut(s)	216, 315, 2171
BplI	GAGNNNNNCTC	4 cut(s)	2468, 2500, 2587, 2619
BpmI	CTGGAG	1 cut(s)	1743
Bpu10I	CCTNAGC	2 cut(s)	929, 1929
Bpu1102I	GCTNAGC	1 cut(s)	1464
BpuEI	CTTGAG	3 cut(s)	402, 1580, 2507
BpuMI	CCSGG	3 cut(s)	603, 604, 1379
BsaAI	YACGTR	3 cut(s)	1757, 2401, 2509
BsaHI	GRCGYC	4 cut(s)	239, 317, 453, 690
BsaI	GGTCTC	1 cut(s)	265
BsaJI	CCNNGG	6 cut(s)	147, 332, 602, 989, 2067, 2167
BsaWI	WCCGGW	7 cut(s)	13, 157, 232, 343, 458, 1016, 1192
BsaXI	ACNNNNNCTCC	4 cut(s)	656, 686, 736, 766
Bse118I	RCCGGY	5 cut(s)	62, 244, 412, 446, 580
Bse1I	ACTGG	4 cut(s)	948, 1703, 1710, 1726
Bse3DI	GCAATG	4 cut(s)	1111, 1307, 1661, 1954
BseAI	TCCGGA	1 cut(s)	1192
BseBI	CCWGG	4 cut(s)	990, 1323, 1805, 2306
BseDI	CCNNGG	6 cut(s)	147, 332, 602, 989, 2067, 2167
BseGI	GGATG	7 cut(s)	965, 1277, 1738, 1783, 1800, 2429, 2536
BseMI	GCAATG	4 cut(s)	1111, 1307, 1661, 1954
BseMII	CTCAG	9 cut(s)	58, 396, 920, 939, 989, 1920, 1989, 2066, 2499
BseNI	ACTGG	4 cut(s)	948, 1703, 1710, 1726
BseRI	GAGGAG	4 cut(s)	32, 117, 963, 1247
BseSI	GKGCMC	4 cut(s)	881, 1190, 1342, 2167
BseX3I	CGGCCG	1 cut(s)	896
BseYI	CCCAGC	1 cut(s)	30
BsgI	GTGCAG	2 cut(s)	116, 1202
Bsh1236I	CGCG	3 cut(s)	265, 334, 557
Bsh1285I	CGRYCG	4 cut(s)	249, 447, 614, 899
BshFI	GGCC	8 cut(s)	560, 593, 898, 1134, 1175, 1242, 1566, 2165
BshNI	GGYRCC	2 cut(s)	452, 2232
BsiEI	CGRYCG	4 cut(s)	249, 447, 614, 899
BsiHKAI	GWGCWC	4 cut(s)	90, 438, 2265, 2473
BsiHKCI	CYCGRG	3 cut(s)	602, 2466, 2472
BsiWI	CGTACG	1 cut(s)	2509
BslFI	GGGAC	4 cut(s)	526, 1615, 2085, 2582
BsmAI	GTCTC	5 cut(s)	265, 296, 1719, 2241, 2379
BsmBI	CGTCTC	1 cut(s)	296
BsmFI	GGGAC	4 cut(s)	526, 1615, 2085, 2582
BsmI	GAATGC	2 cut(s)	1736, 2498
BsnI	GGCC	8 cut(s)	560, 593, 898, 1134, 1175, 1242, 1566, 2165
Bso31I	GGTCTC	1 cut(s)	265
BsoBI	CYCGRG	3 cut(s)	602, 2466, 2472
Bsp120I	GGGCCC	1 cut(s)	2163
Bsp1286I	GDGCHC	8 cut(s)	90, 438, 881, 1190, 1342, 2167, 2265, 2473
Bsp13I	TCCGGA	1 cut(s)	1192
Bsp1720I	GCTNAGC	1 cut(s)	1464
BspANI	GGCC	8 cut(s)	560, 593, 898, 1134, 1175, 1242, 1566, 2165
BspCNI	CTCAG	9 cut(s)	57, 397, 921, 940, 988, 1921, 1990, 2067, 2498
BspEI	TCCGGA	1 cut(s)	1192
BspFNI	CGCG	3 cut(s)	265, 334, 557
BspHI	TCATGA	3 cut(s)	1125, 1518, 1624
BspLI	GGNNCC	6 cut(s)	454, 592, 818, 2165, 2234, 2310
BspMAI	CTGCAG	2 cut(s)	704, 1506
BspMI	ACCTGC	2 cut(s)	627, 1359
BspPI	GGATC	7 cut(s)	149, 376, 583, 811, 824, 1343, 1441
BspQI	GCTCTTC	3 cut(s)	20, 78, 1491
BspT107I	GGYRCC	2 cut(s)	452, 2232
BspTNI	GGTCTC	1 cut(s)	265
BsrDI	GCAATG	4 cut(s)	1111, 1307, 1661, 1954
BsrFI	RCCGGY	5 cut(s)	62, 244, 412, 446, 580
BsrI	ACTGG	4 cut(s)	948, 1703, 1710, 1726
BssAI	RCCGGY	5 cut(s)	62, 244, 412, 446, 580
BssECI	CCNNGG	6 cut(s)	147, 332, 602, 989, 2067, 2167
BssNI	GRCGYC	4 cut(s)	239, 317, 453, 690
BssT1I	CCWWGG	2 cut(s)	147, 2067
Bst2UI	CCWGG	4 cut(s)	990, 1323, 1805, 2306
Bst4CI	ACNGT	1 cut(s)	2171
Bst6I	CTCTTC	7 cut(s)	20, 78, 193, 601, 1393, 1491, 1575
BstACI	GRCGYC	4 cut(s)	239, 317, 453, 690
BstAPI	GCANNNNNTGC	2 cut(s)	639, 708
BstBAI	YACGTR	3 cut(s)	1757, 2401, 2509
BstDSI	CCRYGG	2 cut(s)	332, 2167
BstF5I	GGATG	7 cut(s)	965, 1277, 1738, 1783, 1800, 2429, 2536
BstFNI	CGCG	3 cut(s)	265, 334, 557
BstH2I	RGCGCY	1 cut(s)	456
BstHHI	GCGC	4 cut(s)	68, 455, 557, 2461
BstMAI	GTCTC	5 cut(s)	265, 296, 1719, 2241, 2379
BstMCI	CGRYCG	4 cut(s)	249, 447, 614, 899
BstMWI	GCNNNNNNNGC	8 cut(s)	27, 346, 607, 639, 708, 1465, 2387, 2477
BstNI	CCWGG	4 cut(s)	990, 1323, 1805, 2306
BstSCI	CCNGG	7 cut(s)	601, 602, 988, 1321, 1377, 1803, 2304
BstSFI	CTRYAG	3 cut(s)	700, 1502, 1875
BstSLI	GKGCMC	4 cut(s)	881, 1190, 1342, 2167
BstSNI	TACGTA	1 cut(s)	2509
BstUI	CGCG	3 cut(s)	265, 334, 557
BstV2I	GAAGAC	3 cut(s)	216, 315, 2171
BstX2I	RGATCY	3 cut(s)	816, 1348, 1433
BstXI	CCANNNNNNTGG	1 cut(s)	2174
BstYI	RGATCY	3 cut(s)	816, 1348, 1433
BstZI	CGGCCG	1 cut(s)	896
BsuI	GTATCC	1 cut(s)	1189
BsuRI	GGCC	8 cut(s)	560, 593, 898, 1134, 1175, 1242, 1566, 2165
BtgI	CCRYGG	2 cut(s)	332, 2167
BtgZI	GCGATG	2 cut(s)	88, 737
BtrI	CACGTC	1 cut(s)	1995
BtsCI	GGATG	7 cut(s)	965, 1277, 1738, 1783, 1800, 2429, 2536
BtsI	GCAGTG	2 cut(s)	1190, 2127
BtsIMutI	CAGTG	2 cut(s)	1190, 2127
BveI	ACCTGC	2 cut(s)	627, 1359
CaiI	CAGNNNCTG	1 cut(s)	2377
CciI	TCATGA	3 cut(s)	1125, 1518, 1624
CfoI	GCGC	4 cut(s)	68, 455, 557, 2461
Cfr10I	RCCGGY	5 cut(s)	62, 244, 412, 446, 580
Cfr13I	GGNCC	6 cut(s)	37, 591, 1564, 2163, 2164, 2518
Cfr42I	CCGCGG	1 cut(s)	335
Cfr9I	CCCGGG	1 cut(s)	602
CseI	GACGC	3 cut(s)	228, 346, 698
CsiI	ACCWGGT	2 cut(s)	1321, 2304
Csp6I	GTAC	6 cut(s)	697, 1003, 1316, 2228, 2506, 2510
CviAII	CATG	5 cut(s)	1126, 1519, 1625, 1714, 2360
CviQI	GTAC	6 cut(s)	697, 1003, 1316, 2228, 2506, 2510
DinI	GGCGCC	1 cut(s)	454
EaeI	YGGCCR	4 cut(s)	558, 896, 1132, 1173
EagI	CGGCCG	1 cut(s)	896
Eam1104I	CTCTTC	7 cut(s)	20, 78, 193, 601, 1393, 1491, 1575
EarI	CTCTTC	7 cut(s)	20, 78, 193, 601, 1393, 1491, 1575
Ecl136II	GAGCTC	1 cut(s)	2471
EclXI	CGGCCG	1 cut(s)	896
Eco105I	TACGTA	1 cut(s)	2509
Eco130I	CCWWGG	2 cut(s)	147, 2067
Eco24I	GRGCYC	2 cut(s)	2167, 2473
Eco31I	GGTCTC	1 cut(s)	265
Eco32I	GATATC	2 cut(s)	1608, 1711
Eco47I	GGWCC	2 cut(s)	37, 2518
Eco52I	CGGCCG	1 cut(s)	896
Eco53kI	GAGCTC	1 cut(s)	2471
Eco57I	CTGAAG	4 cut(s)	737, 1620, 1866, 2326
Eco72I	CACGTG	1 cut(s)	1757
Eco88I	CYCGRG	3 cut(s)	602, 2466, 2472
EcoICRI	GAGCTC	1 cut(s)	2471
EcoO109I	RGGNCCY	3 cut(s)	37, 591, 2518
EcoRI	GAATTC	1 cut(s)	1382
EcoRII	CCWGG	4 cut(s)	988, 1321, 1803, 2304
EcoRV	GATATC	2 cut(s)	1608, 1711
EcoT14I	CCWWGG	2 cut(s)	147, 2067
EcoT22I	ATGCAT	2 cut(s)	1661, 1738
EcoT38I	GRGCYC	2 cut(s)	2167, 2473
EgeI	GGCGCC	1 cut(s)	454
EheI	GGCGCC	1 cut(s)	454
ErhI	CCWWGG	2 cut(s)	147, 2067
Esp3I	CGTCTC	1 cut(s)	296
FaeI	CATG	5 cut(s)	1129, 1522, 1628, 1717, 2363
FaqI	GGGAC	4 cut(s)	526, 1615, 2085, 2582
FatI	CATG	5 cut(s)	1125, 1518, 1624, 1713, 2359
FauI	CCCGC	5 cut(s)	14, 150, 552, 635, 889
FauNDI	CATATG	1 cut(s)	2608
FbaI	TGATCA	1 cut(s)	2152
FokI	GGATG	7 cut(s)	972, 1264, 1745, 1787, 1790, 2416, 2543
FriOI	GRGCYC	2 cut(s)	2167, 2473
FspBI	CTAG	2 cut(s)	1443, 1832
GlaI	GCGC	4 cut(s)	67, 454, 556, 2460
GsaI	CCCAGC	1 cut(s)	34
GsuI	CTGGAG	1 cut(s)	1743
HaeII	RGCGCY	1 cut(s)	456
HaeIII	GGCC	8 cut(s)	560, 593, 898, 1134, 1175, 1242, 1566, 2165
HgaI	GACGC	3 cut(s)	228, 346, 698
HhaI	GCGC	4 cut(s)	68, 455, 557, 2461
Hin1I	GRCGYC	4 cut(s)	239, 317, 453, 690
Hin1II	CATG	5 cut(s)	1129, 1522, 1628, 1717, 2363
Hin6I	GCGC	4 cut(s)	66, 453, 555, 2459
HinP1I	GCGC	4 cut(s)	66, 453, 555, 2459
HincII	GTYRAC	2 cut(s)	163, 2491
HindII	GTYRAC	2 cut(s)	163, 2491
HpaI	GTTAAC	1 cut(s)	163
HphI	GGTGA	4 cut(s)	289, 536, 1049, 2419
Hpy166II	GTNNAC	3 cut(s)	163, 1488, 2491
Hpy188III	TCNNGA	9 cut(s)	176, 419, 1126, 1193, 1437, 1519, 1625, 1844, 2474
Hpy8I	GTNNAC	3 cut(s)	163, 1488, 2491
Hpy99I	CGWCG	6 cut(s)	83, 113, 244, 319, 322, 692
HpyCH4III	ACNGT	1 cut(s)	2171
HpyCH4IV	ACGT	6 cut(s)	317, 1573, 1756, 1994, 2400, 2508
HpyF10VI	GCNNNNNNNGC	8 cut(s)	27, 346, 607, 639, 708, 1465, 2387, 2477
HpySE526I	ACGT	6 cut(s)	317, 1573, 1756, 1994, 2400, 2508
Hsp92I	GRCGYC	4 cut(s)	239, 317, 453, 690
Hsp92II	CATG	5 cut(s)	1129, 1522, 1628, 1717, 2363
HspAI	GCGC	4 cut(s)	66, 453, 555, 2459
KasI	GGCGCC	1 cut(s)	452
Kpn2I	TCCGGA	1 cut(s)	1192
KroI	GCCGGC	2 cut(s)	62, 412
KroNI	GCCGGC	2 cut(s)	64, 414
Ksp22I	TGATCA	1 cut(s)	2152
KspAI	GTTAAC	1 cut(s)	163
KspI	CCGCGG	1 cut(s)	335
LguI	GCTCTTC	3 cut(s)	20, 78, 1491
LmnI	GCTCC	5 cut(s)	346, 383, 433, 901, 1771
LweI	GCATC	7 cut(s)	42, 723, 1087, 1286, 1668, 1723, 2272
MabI	ACCWGGT	2 cut(s)	1321, 2304
MaeI	CTAG	2 cut(s)	1443, 1832
MaeII	ACGT	6 cut(s)	317, 1573, 1756, 1994, 2400, 2508
MaeIII	GTNAC	6 cut(s)	196, 281, 481, 1701, 1889, 1936
MfeI	CAATTG	1 cut(s)	706
MflI	RGATCY	3 cut(s)	816, 1348, 1433
MhlI	GDGCHC	8 cut(s)	90, 438, 881, 1190, 1342, 2167, 2265, 2473
MlsI	TGGCCA	1 cut(s)	1175
MluNI	TGGCCA	1 cut(s)	1175
Mly113I	GGCGCC	1 cut(s)	453
MlyI	GAGTC	7 cut(s)	164, 322, 416, 530, 791, 1637, 2567
Mox20I	TGGCCA	1 cut(s)	1175
Mph1103I	ATGCAT	2 cut(s)	1661, 1738
MroI	TCCGGA	1 cut(s)	1192
MroNI	GCCGGC	2 cut(s)	62, 412
MroXI	GAANNNNTTC	1 cut(s)	1484
MscI	TGGCCA	1 cut(s)	1175
MseI	TTAA	3 cut(s)	162, 1254, 2061
MslI	CAYNNNNRTG	3 cut(s)	1118, 2172, 2591
Msp20I	TGGCCA	1 cut(s)	1175
MspA1I	CMGCKG	2 cut(s)	203, 334
MspR9I	CCNGG	7 cut(s)	603, 604, 990, 1323, 1379, 1805, 2306
MunI	CAATTG	1 cut(s)	706
Mva1269I	GAATGC	2 cut(s)	1736, 2498
MvaI	CCWGG	4 cut(s)	990, 1323, 1805, 2306
MvnI	CGCG	3 cut(s)	265, 334, 557
MwoI	GCNNNNNNNGC	8 cut(s)	27, 346, 607, 639, 708, 1465, 2387, 2477
NaeI	GCCGGC	2 cut(s)	64, 414
NarI	GGCGCC	1 cut(s)	453
NciI	CCSGG	3 cut(s)	603, 604, 1379
NdeI	CATATG	1 cut(s)	2608
NgoMIV	GCCGGC	2 cut(s)	62, 412
NlaIII	CATG	5 cut(s)	1129, 1522, 1628, 1717, 2363
NlaIV	GGNNCC	6 cut(s)	454, 592, 818, 2165, 2234, 2310
NmeAIII	GCCGAG	2 cut(s)	1160, 1592
NmuCI	GTSAC	2 cut(s)	196, 481
NsiI	ATGCAT	2 cut(s)	1661, 1738
OliI	CACNNNNGTG	1 cut(s)	1118
PagI	TCATGA	3 cut(s)	1125, 1518, 1624
PaqCI	CACCTGC	1 cut(s)	627
PciSI	GCTCTTC	3 cut(s)	20, 78, 1491
PcsI	WCGNNNNNNNCGW	3 cut(s)	174, 311, 323
PctI	GAATGC	2 cut(s)	1736, 2498
PdiI	GCCGGC	2 cut(s)	64, 414
PdmI	GAANNNNTTC	1 cut(s)	1484
PfeI	GAWTC	7 cut(s)	229, 503, 847, 1013, 1087, 1522, 1793
Pfl23II	CGTACG	1 cut(s)	2509
PflFI	GACNNNGTC	1 cut(s)	319
PfoI	TCCNGGA	2 cut(s)	1377, 1803
Ple19I	CGATCG	1 cut(s)	614
PleI	GAGTC	7 cut(s)	164, 322, 416, 530, 790, 1636, 2567
PluTI	GGCGCC	1 cut(s)	456
PmaCI	CACGTG	1 cut(s)	1757
PmlI	CACGTG	1 cut(s)	1757
PpsI	GAGTC	7 cut(s)	164, 322, 416, 530, 790, 1636, 2567
Ppu21I	YACGTR	3 cut(s)	1757, 2401, 2509
PpuMI	RGGWCCY	2 cut(s)	37, 2518
PshBI	ATTAAT	1 cut(s)	2061
Psp124BI	GAGCTC	1 cut(s)	2473
Psp1406I	AACGTT	1 cut(s)	1573
Psp5II	RGGWCCY	2 cut(s)	37, 2518
Psp6I	CCWGG	4 cut(s)	988, 1321, 1803, 2304
PspCI	CACGTG	1 cut(s)	1757
PspFI	CCCAGC	1 cut(s)	30
PspGI	CCWGG	4 cut(s)	988, 1321, 1803, 2304
PspLI	CGTACG	1 cut(s)	2509
PspN4I	GGNNCC	6 cut(s)	454, 592, 818, 2165, 2234, 2310
PspOMI	GGGCCC	1 cut(s)	2163
PspPI	GGNCC	6 cut(s)	37, 591, 1564, 2163, 2164, 2518
PspPPI	RGGWCCY	2 cut(s)	37, 2518
PstI	CTGCAG	2 cut(s)	704, 1506
PstNI	CAGNNNCTG	1 cut(s)	2377
PsuI	RGATCY	3 cut(s)	816, 1348, 1433
PsyI	GACNNNGTC	1 cut(s)	319
PvuI	CGATCG	1 cut(s)	614
RsaI	GTAC	6 cut(s)	698, 1004, 1317, 2229, 2507, 2511
RsaNI	GTAC	6 cut(s)	697, 1003, 1316, 2228, 2506, 2510
RseI	CAYNNNNRTG	3 cut(s)	1118, 2172, 2591
SacI	GAGCTC	1 cut(s)	2473
SacII	CCGCGG	1 cut(s)	335
SapI	GCTCTTC	3 cut(s)	20, 78, 1491
SaqAI	TTAA	3 cut(s)	162, 1254, 2061
Sau96I	GGNCC	6 cut(s)	37, 591, 1564, 2163, 2164, 2518
ScaI	AGTACT	1 cut(s)	698
SchI	GAGTC	7 cut(s)	164, 322, 416, 530, 791, 1637, 2567
ScrFI	CCNGG	7 cut(s)	603, 604, 990, 1323, 1379, 1805, 2306
SduI	GDGCHC	8 cut(s)	90, 438, 881, 1190, 1342, 2167, 2265, 2473
SexAI	ACCWGGT	2 cut(s)	1321, 2304
SfaNI	GCATC	7 cut(s)	42, 723, 1087, 1286, 1668, 1723, 2272
SfcI	CTRYAG	3 cut(s)	700, 1502, 1875
SfoI	GGCGCC	1 cut(s)	454
Sfr303I	CCGCGG	1 cut(s)	335
SgrBI	CCGCGG	1 cut(s)	335
SinI	GGWCC	2 cut(s)	37, 2518
SmaI	CCCGGG	1 cut(s)	604
SmiMI	CAYNNNNRTG	3 cut(s)	1118, 2172, 2591
SmlI	CTYRAG	3 cut(s)	417, 1559, 2522
SmoI	CTYRAG	3 cut(s)	417, 1559, 2522
SnaBI	TACGTA	1 cut(s)	2509
SspDI	GGCGCC	1 cut(s)	452
SspI	AATATT	1 cut(s)	1859
SspMI	CTAG	2 cut(s)	1443, 1832
SstI	GAGCTC	1 cut(s)	2473
StyD4I	CCNGG	7 cut(s)	601, 602, 988, 1321, 1377, 1803, 2304
StyI	CCWWGG	2 cut(s)	147, 2067
TaaI	ACNGT	1 cut(s)	2171
TaiI	ACGT	6 cut(s)	320, 1576, 1759, 1997, 2403, 2511
TaqII	GACCGA	1 cut(s)	239
TatI	WGTACW	1 cut(s)	696
TauI	GCSGC	5 cut(s)	291, 453, 476, 560, 2442
TfiI	GAWTC	7 cut(s)	229, 503, 847, 1013, 1087, 1522, 1793
Tru1I	TTAA	3 cut(s)	162, 1254, 2061
Tru9I	TTAA	3 cut(s)	162, 1254, 2061
TscAI	CASTG	2 cut(s)	1190, 2127
TseFI	GTSAC	2 cut(s)	196, 481
Tsp45I	GTSAC	2 cut(s)	196, 481
TspGWI	ACGGA	4 cut(s)	184, 2244, 2301, 2603
TspMI	CCCGGG	1 cut(s)	602
TspRI	CASTG	2 cut(s)	1190, 2127
Tth111I	GACNNNGTC	1 cut(s)	319
VpaK11BI	GGWCC	2 cut(s)	37, 2518
VspI	ATTAAT	1 cut(s)	2061
XapI	RAATTY	4 cut(s)	374, 1382, 1967, 2411
XcmI	CCANNNNNNNNNTGG	1 cut(s)	568
XmaI	CCCGGG	1 cut(s)	602
XmnI	GAANNNNTTC	1 cut(s)	1484
XspI	CTAG	2 cut(s)	1443, 1832
ZraI	GACGTC	1 cut(s)	318
ZrmI	AGTACT	1 cut(s)	698
Zsp2I	ATGCAT	2 cut(s)	1661, 1738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.