RchiOBHm_Chr7g0177881

Extra-large guanine nucleotide-binding protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003471
Physical Location & Seq
Forward (+)
683111 .. 687682
4572 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15849

Sequence Viewer

Length: 2556 bp
ATGGCGGCTCCGGAACCCGAGGACGAGAAAAGCTGGCAGGAGCTACTCAGAAAGATGCTGCCTTCCGGCGTGCCGTTTCCGGACGACGACCATCTCGACTACTCCATCGCAGTTGAGTACGAAGGCCCTCCGCTGCCCTACGAAGTCCCCAAGGTTGATCCAGTCGACATCGACTCGCTTCACAACGCTGCGTCCGGAGCTTCGGGTTCGGGTTCGGAGCTTTCTTCGATTCCGGTGGCCGAGCCGGTCGATCGGGACGCCTCTCGGTTCAGCCGGATCAGGAACGGCGACGCCGCAAACGAAACGCAGACTTCGACGTCGTTTTCGGTAGGTTTCAGCTCTGAATTTTCAGCTCAGGACTCGAACTCGGAGCCGAAACCGGTCGAGGACGACGCCGGAGGAGGAGGAAAGAGAGCGGCGGTCGTGACTTTCAACACGCCGAGAGAATCGGAGACGGAAGACGACGGCGACCGGTCCTCGTCGCCGCAATCGGAGCCGGTGGGATCGCCGGTTGCTTGGGCCTCGCCGCTGAAGGTAGCGAGTAAGAGAGGGATTTGCAGCAGGTGTGGAAAAGGGAACAGATTGAAGGAGAGGGAGTGGTGCTTGGTGTGCGACTCAAAGTACTGTAGCAACTGTTTGCTTAAAGCTATGGGATCAATGCCGGAGGGGCGAAAATGTGTGAGCTGCATTGGGCAGCCTATAGATGAGTCCAAGAGGCCTAGTTTGGGCAAGTGTTCAAAGCTTCTTTCTAGGGTTTGTAGTCCTTTGGAGATTGGGCAGATAATGAGGGCCGAGAAGGAGTGTCCGGCCAATCAGCTCCGGCCACAGCAGTTGGTTGTGAATGGGAGGGAGTTGAGGGAAGAAGAGCTGGGTGAGATTTTGGGATGTGAAGTACCTCCTCAGAAGTTGAAGCCTGGGAGGTACTGGTACGATAAGGATTCAGGCCTCTGGGGAAAGGAGGGAGAGAAGCCTGATTGTATCATTACTCCTAAACTGAATGTTGGGGGTAAGCTTCGGGCTGATGCCAGCAATGGGAACACTAATGTCTTCATGAATGGCAGAGAGATCACAAAGATTGAGCTTAGGGTGCTCAAGCTGGCCAATGTGCAGTGCCCTCCAGATACTCATTTCTGGGTGTATGAAGATGGTACATATGAGGAGGAAGGGCAGAATAACATTAAAGGAAACATATGGGGAAAGGCAACCACCCGTTTCATGTGTTCATTATTCTCATTGCCTGTACCATCTCGTAATCAAAATGGGGCAAGAGAAGATCCTATGACACAACCTTCAAGTAGGTCCATCCCTGAATACTTGGATCAGGCAAGAGTTCAAAAACTTTTGTTATTTGGTCTGGAGGGATCTGGAACTAGCACCATCTTCAAGCAGGCCAAGTTCTTATATGGGAACGAGTTCACTCCCGAGGAGGTGCAGAACATGAAGCTCATGATTCAAAGCAATATGTACAAGTACTTAAGTATATTACTTGAGGGCCGAGAACGTTTTGAAGAGGAAGCTTCATTGCAGAATACTTCTGATTCCTTGGCCACTGACGAGACGCCATCAGTAGTCGATGGAAGTAAGCAATGCATCTATTCTATCAACCAAAGATTCAAGCACTTTTCTGACTGGTTGCTGGATATCATGGCAACCGGAGACTTGGATGCATTCTTCCCTGCTGCTACACGTGAGTATGCTCCTATTGTAGATGAAGTATGGAAAGATTCTGCCATCCAGGAAACATACAAAAGAAGGGAGGAATTACATTGTCTTCCAGAAGTTGCCAAGTATTTCTTAGATCGGGCTATAGAAATATCAAGTAATGAGTATGAGCCAACCGAGAAGGACATTTTATATGCTGAAGGAGTTACTCAAAGCAATGGCCTTACCTCTCTAGAATTTTCATTTGATGATAGAAGCTCCATGTCTGAGCTATACAACGAAAACTATGAATGCCCCCTTCCGTTAACCAAGTACCAACTAATTTGTCTCAGTTCTAAGGGACTAAATGAGGGGTGCAAATGGCTTGCAATGTTTGAAGACGTGAGGGCAGTGATTTTTTGTGTTGCTTTGAGTGACTATGATCAAATGTGGGCCCTTGGTAATGGGCGTCTTCATAATAAATTGCTGGCAAGCAGAGATTTGTTCGAGAGTTTGGTGAGGCACCCTTGTTTTAGTAACACTCCATTTGTTCTTGTGCTGAACAAATATGATGCATTTGAAGACAAGATAGGCCAGGTTCCATTATCAGCTTGTGAGTGGTTCAATGACTTCAACCCTGTTAAGCCGCACAATAATAGTCAGGCTCTAGCTCAGCAAGCATATTACTATGTGGCAGTGAAATTCAAAGAGCTCTATTTATCCTTAAGCGGTCAAAAGTTATTTGTAGTGCAAACCCGAGCTAGAGATGGTAACTCGGTTGATGTGGCATTCAAGTACATAAGGGAGGTCCTCAAGTGGGATGAAGAAAAGGATGATAATTTCTTTGGAATAAATGGGGATGACTCCTATTTCAGTACAGAAATGAGTTCTTCTCCATATCTCAGGCAAGAATAA

Protein Analysis

851

Amino Acids

95.51

Weight (kDa)

4.89

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 442 - 817 2.8e-72 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 552
AasI GACNNNNNNGTC 1 cut(s) 316
AatII GACGTC 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 552
AccB1I GGYRCC 1 cut(s) 2163
AccBSI CCGCTC 1 cut(s) 416
AccI GTMKAC 1 cut(s) 165
AccIII TCCGGA 3 cut(s) 10, 79, 194
AciI CCGC 9 cut(s) 5, 131, 294, 416, 419, 485, 527, 2288, 2370
AclI AACGTT 1 cut(s) 1501
AclWI GGATC 7 cut(s) 152, 284, 511, 661, 1268, 1326, 1369
AcoI YGGCCR 5 cut(s) 237, 807, 821, 1098, 1545
AcsI RAATTY 3 cut(s) 344, 1898, 2342
AcuI CTGAAG 2 cut(s) 551, 1881
AcvI CACGTG 1 cut(s) 1688
AcyI GRCGYC 6 cut(s) 258, 291, 317, 393, 1559, 2110
AfiI CCNNNNNNNGG 3 cut(s) 725, 1032, 2458
AflII CTTAAG 2 cut(s) 1474, 2365
AflIII ACRYGT 1 cut(s) 1685
AgeI ACCGGT 2 cut(s) 379, 471
AjiI CACGTC 1 cut(s) 2044
AjnI CCWGG 3 cut(s) 913, 1734, 2235
Alw21I GWGCWC 2 cut(s) 1092, 2355
Alw26I GTCTC 4 cut(s) 446, 1550, 1650, 1994
AlwI GGATC 7 cut(s) 152, 284, 511, 661, 1268, 1326, 1369
Ama87I CYCGRG 3 cut(s) 17, 1421, 2397
Aor13HI TCCGGA 3 cut(s) 10, 79, 194
ApaI GGGCCC 1 cut(s) 2098
ApeKI GCWGC 7 cut(s) 58, 133, 188, 558, 684, 694, 1679
ApoI RAATTY 3 cut(s) 344, 1898, 2342
ArsI GACNNNNNNTTYG 2 cut(s) 307, 339
AsiGI ACCGGT 2 cut(s) 379, 471
Asp700I GAANNNNTTC 1 cut(s) 1412
AspS9I GGNCC 9 cut(s) 125, 474, 519, 789, 1299, 1492, 2094, 2095, 2449
AsuHPI GGTGA 2 cut(s) 884, 2170
AvaI CYCGRG 3 cut(s) 17, 1421, 2397
AvaII GGWCC 3 cut(s) 474, 1299, 2449
BaeGI GKGCMC 2 cut(s) 1115, 2098
BalI TGGCCA 2 cut(s) 1100, 1547
BanI GGYRCC 1 cut(s) 2163
BanII GRGCYC 2 cut(s) 2098, 2355
BbrPI CACGTG 1 cut(s) 1688
BbsI GAAGAC 6 cut(s) 465, 1039, 1763, 2046, 2105, 2229
Bbv12I GWGCWC 2 cut(s) 1092, 2355
BbvI GCAGC 7 cut(s) 45, 120, 175, 570, 671, 706, 1666
BceAI ACGGC 3 cut(s) 58, 301, 481
BciT130I CCWGG 3 cut(s) 915, 1736, 2237
BclI TGATCA 1 cut(s) 2083
BcoDI GTCTC 4 cut(s) 446, 1550, 1650, 1994
BfaI CTAG 6 cut(s) 720, 750, 1371, 1895, 2309, 2403
BfmI CTRYAG 3 cut(s) 625, 699, 1806
BfrI CTTAAG 2 cut(s) 1474, 2365
BfuAI ACCTGC 1 cut(s) 552
BglI GCCNNNNNGGC 1 cut(s) 667
BlpI GCTNAGC 1 cut(s) 2313
BmcAI AGTACT 2 cut(s) 623, 1472
Bme1390I CCNGG 3 cut(s) 915, 1736, 2237
Bme18I GGWCC 3 cut(s) 474, 1299, 2449
BmeT110I CYCGRG 3 cut(s) 17, 1421, 2397
BmgBI CACGTC 1 cut(s) 2044
BmgT120I GGNCC 9 cut(s) 125, 474, 519, 789, 1299, 1492, 2094, 2095, 2449
BmiI GGNNCC 7 cut(s) 9, 15, 372, 495, 2096, 2165, 2241
BmrFI CCNGG 3 cut(s) 915, 1736, 2237
BmsI GCATC 5 cut(s) 45, 1012, 1599, 1654, 2203
BpiI GAAGAC 6 cut(s) 465, 1039, 1763, 2046, 2105, 2229
BplI GAGNNNNNCTC 2 cut(s) 2518, 2550
BpmI CTGGAG 2 cut(s) 1101, 1376
Bpu10I CCTNAGC 2 cut(s) 354, 1082
Bpu1102I GCTNAGC 1 cut(s) 2313
BpuEI CTTGAG 3 cut(s) 1076, 1508, 2438
BsaAI YACGTR 1 cut(s) 1688
BsaHI GRCGYC 6 cut(s) 258, 291, 317, 393, 1559, 2110
BsaJI CCNNGG 6 cut(s) 18, 150, 914, 1422, 1542, 2098
BsaWI WCCGGW 7 cut(s) 10, 79, 194, 232, 379, 471, 1652
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 3 cut(s) 725, 1032, 2458
Bse118I RCCGGY 5 cut(s) 244, 379, 471, 496, 508
Bse1I ACTGG 3 cut(s) 161, 929, 1634
Bse3DI GCAATG 6 cut(s) 1036, 1232, 1520, 1592, 1885, 2037
BseAI TCCGGA 3 cut(s) 10, 79, 194
BseBI CCWGG 3 cut(s) 915, 1736, 2237
BseDI CCNNGG 6 cut(s) 18, 150, 914, 1422, 1542, 2098
BseGI GGATG 7 cut(s) 890, 1302, 1669, 1731, 2467, 2479, 2506
BseLI CCNNNNNNNGG 3 cut(s) 725, 1032, 2458
BseMI GCAATG 6 cut(s) 1036, 1232, 1520, 1592, 1885, 2037
BseMII CTCAG 6 cut(s) 61, 368, 914, 1920, 2005, 2327
BseNI ACTGG 3 cut(s) 161, 929, 1634
BseRI GAGGAG 5 cut(s) 414, 417, 888, 1172, 1439
BseSI GKGCMC 2 cut(s) 1115, 2098
BseXI GCAGC 7 cut(s) 45, 120, 175, 570, 671, 706, 1666
BseYI CCCAGC 1 cut(s) 868
BsgI GTGCAG 2 cut(s) 1127, 1451
Bsh1285I CGRYCG 5 cut(s) 249, 253, 384, 423, 472
BshNI GGYRCC 1 cut(s) 2163
BshTI ACCGGT 2 cut(s) 379, 471
BsiEI CGRYCG 5 cut(s) 249, 253, 384, 423, 472
BsiHKAI GWGCWC 2 cut(s) 1092, 2355
BsiHKCI CYCGRG 3 cut(s) 17, 1421, 2397
BslFI GGGAC 3 cut(s) 131, 269, 2016
BslI CCNNNNNNNGG 3 cut(s) 725, 1032, 2458
BsmAI GTCTC 4 cut(s) 446, 1550, 1650, 1994
BsmBI CGTCTC 2 cut(s) 446, 1550
BsmFI GGGAC 3 cut(s) 131, 269, 2016
BsmI GAATGC 3 cut(s) 1667, 1958, 2429
BsoBI CYCGRG 3 cut(s) 17, 1421, 2397
Bsp120I GGGCCC 1 cut(s) 2094
Bsp1286I GDGCHC 4 cut(s) 1092, 1115, 2098, 2355
Bsp13I TCCGGA 3 cut(s) 10, 79, 194
Bsp1407I TGTACA 1 cut(s) 1464
Bsp1720I GCTNAGC 1 cut(s) 2313
BspACI CCGC 9 cut(s) 5, 131, 294, 416, 419, 485, 527, 2288, 2370
BspCNI CTCAG 7 cut(s) 60, 367, 913, 1921, 2004, 2326, 2556
BspEI TCCGGA 3 cut(s) 10, 79, 194
BspHI TCATGA 2 cut(s) 1050, 1446
BspLI GGNNCC 7 cut(s) 9, 15, 372, 495, 2096, 2165, 2241
BspMI ACCTGC 1 cut(s) 552
BspPI GGATC 7 cut(s) 152, 284, 511, 661, 1268, 1326, 1369
BspQI GCTCTTC 1 cut(s) 858
BspT107I GGYRCC 1 cut(s) 2163
BspTI CTTAAG 2 cut(s) 1474, 2365
BsrBI CCGCTC 1 cut(s) 416
BsrDI GCAATG 6 cut(s) 1036, 1232, 1520, 1592, 1885, 2037
BsrFI RCCGGY 5 cut(s) 244, 379, 471, 496, 508
BsrGI TGTACA 1 cut(s) 1464
BsrI ACTGG 3 cut(s) 161, 929, 1634
BssAI RCCGGY 5 cut(s) 244, 379, 471, 496, 508
BssECI CCNNGG 6 cut(s) 18, 150, 914, 1422, 1542, 2098
BssNI GRCGYC 6 cut(s) 258, 291, 317, 393, 1559, 2110
BssT1I CCWWGG 3 cut(s) 150, 1542, 2098
Bst2UI CCWGG 3 cut(s) 915, 1736, 2237
Bst4CI ACNGT 2 cut(s) 626, 635
Bst6I CTCTTC 2 cut(s) 858, 1503
BstACI GRCGYC 6 cut(s) 258, 291, 317, 393, 1559, 2110
BstAFI CTTAAG 2 cut(s) 1474, 2365
BstAUI TGTACA 1 cut(s) 1464
BstBAI YACGTR 1 cut(s) 1688
BstC8I GCNNGC 9 cut(s) 35, 71, 1027, 1098, 1389, 2028, 2130, 2134, 2319
BstF5I GGATG 7 cut(s) 890, 1302, 1669, 1731, 2467, 2479, 2506
BstMAI GTCTC 4 cut(s) 446, 1550, 1650, 1994
BstMCI CGRYCG 5 cut(s) 249, 253, 384, 423, 472
BstMWI GCNNNNNNNGC 6 cut(s) 197, 493, 609, 667, 1087, 2318
BstNI CCWGG 3 cut(s) 915, 1736, 2237
BstSCI CCNGG 3 cut(s) 913, 1734, 2235
BstSFI CTRYAG 3 cut(s) 625, 699, 1806
BstSLI GKGCMC 2 cut(s) 1115, 2098
BstV1I GCAGC 7 cut(s) 45, 120, 175, 570, 671, 706, 1666
BstV2I GAAGAC 6 cut(s) 465, 1039, 1763, 2046, 2105, 2229
BstX2I RGATCY 2 cut(s) 1273, 1361
BstYI RGATCY 2 cut(s) 1273, 1361
BtgZI GCGATG 1 cut(s) 91
BtrI CACGTC 1 cut(s) 2044
BtsCI GGATG 7 cut(s) 890, 1302, 1669, 1731, 2467, 2479, 2506
BtsI GCAGTG 3 cut(s) 1115, 2058, 2343
BtsIMutI CAGTG 4 cut(s) 1115, 1548, 2058, 2343
BveI ACCTGC 1 cut(s) 552
Cac8I GCNNGC 9 cut(s) 35, 71, 1027, 1098, 1389, 2028, 2130, 2134, 2319
CciI TCATGA 2 cut(s) 1050, 1446
Cfr10I RCCGGY 5 cut(s) 244, 379, 471, 496, 508
Cfr13I GGNCC 9 cut(s) 125, 474, 519, 789, 1299, 1492, 2094, 2095, 2449
CseI GACGC 6 cut(s) 180, 266, 299, 401, 1567, 2099
CspAI ACCGGT 2 cut(s) 379, 471
CspCI CAANNNNNGTGG 2 cut(s) 813, 848
CviAII CATG 6 cut(s) 1051, 1216, 1438, 1447, 1645, 1924
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
EaeI YGGCCR 5 cut(s) 237, 807, 821, 1098, 1545
Eam1104I CTCTTC 2 cut(s) 858, 1503
EarI CTCTTC 2 cut(s) 858, 1503
Ecl136II GAGCTC 1 cut(s) 2353
Eco130I CCWWGG 3 cut(s) 150, 1542, 2098
Eco147I AGGCCT 2 cut(s) 718, 945
Eco24I GRGCYC 2 cut(s) 2098, 2355
Eco32I GATATC 1 cut(s) 1642
Eco47I GGWCC 3 cut(s) 474, 1299, 2449
Eco53kI GAGCTC 1 cut(s) 2353
Eco57I CTGAAG 2 cut(s) 551, 1881
Eco72I CACGTG 1 cut(s) 1688
Eco88I CYCGRG 3 cut(s) 17, 1421, 2397
EcoICRI GAGCTC 1 cut(s) 2353
EcoO109I RGGNCCY 3 cut(s) 125, 2095, 2449
EcoRII CCWGG 3 cut(s) 913, 1734, 2235
EcoRV GATATC 1 cut(s) 1642
EcoT14I CCWWGG 3 cut(s) 150, 1542, 2098
EcoT22I ATGCAT 3 cut(s) 1592, 1669, 2218
EcoT38I GRGCYC 2 cut(s) 2098, 2355
ErhI CCWWGG 3 cut(s) 150, 1542, 2098
Esp3I CGTCTC 2 cut(s) 446, 1550
FaeI CATG 6 cut(s) 1054, 1219, 1441, 1450, 1648, 1927
FalI AAGNNNNNCTT 2 cut(s) 1778, 1810
FaqI GGGAC 3 cut(s) 131, 269, 2016
FatI CATG 6 cut(s) 1050, 1215, 1437, 1446, 1644, 1923
FauNDI CATATG 2 cut(s) 1153, 1190
FbaI TGATCA 1 cut(s) 2083
FblI GTMKAC 1 cut(s) 165
FokI GGATG 7 cut(s) 897, 1289, 1676, 1718, 2474, 2486, 2513
FriOI GRGCYC 2 cut(s) 2098, 2355
FspBI CTAG 6 cut(s) 720, 750, 1371, 1895, 2309, 2403
GsaI CCCAGC 1 cut(s) 872
GsuI CTGGAG 2 cut(s) 1101, 1376
HgaI GACGC 6 cut(s) 180, 266, 299, 401, 1567, 2099
Hin1I GRCGYC 6 cut(s) 258, 291, 317, 393, 1559, 2110
Hin1II CATG 6 cut(s) 1054, 1219, 1441, 1450, 1648, 1927
HincII GTYRAC 2 cut(s) 166, 1968
HindII GTYRAC 2 cut(s) 166, 1968
HindIII AAGCTT 3 cut(s) 740, 1010, 1515
HpaI GTTAAC 1 cut(s) 1968
HphI GGTGA 2 cut(s) 884, 2170
Hpy166II GTNNAC 3 cut(s) 166, 1416, 1968
Hpy8I GTNNAC 3 cut(s) 166, 1416, 1968
Hpy99I CGWCG 7 cut(s) 89, 293, 319, 322, 395, 467, 484
HpyCH4III ACNGT 2 cut(s) 626, 635
HpyCH4IV ACGT 4 cut(s) 317, 1501, 1687, 2043
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 493, 609, 667, 1087, 2318
HpySE526I ACGT 4 cut(s) 317, 1501, 1687, 2043
Hsp92I GRCGYC 6 cut(s) 258, 291, 317, 393, 1559, 2110
Hsp92II CATG 6 cut(s) 1054, 1219, 1441, 1450, 1648, 1927
Kpn2I TCCGGA 3 cut(s) 10, 79, 194
Ksp22I TGATCA 1 cut(s) 2083
KspAI GTTAAC 1 cut(s) 1968
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 9 cut(s) 13, 40, 197, 217, 370, 493, 822, 1702, 1925
Lsp1109I GCAGC 7 cut(s) 45, 120, 175, 570, 671, 706, 1666
LweI GCATC 5 cut(s) 45, 1012, 1599, 1654, 2203
MaeI CTAG 6 cut(s) 720, 750, 1371, 1895, 2309, 2403
MaeII ACGT 4 cut(s) 317, 1501, 1687, 2043
MaeIII GTNAC 5 cut(s) 424, 1867, 2075, 2177, 2411
MbiI CCGCTC 1 cut(s) 416
MflI RGATCY 2 cut(s) 1273, 1361
MhlI GDGCHC 4 cut(s) 1092, 1115, 2098, 2355
MlsI TGGCCA 2 cut(s) 1100, 1547
MluCI AATT 7 cut(s) 344, 1760, 1898, 1983, 2123, 2342, 2479
MluNI TGGCCA 2 cut(s) 1100, 1547
MlyI GAGTC 5 cut(s) 167, 353, 608, 716, 2498
Mox20I TGGCCA 2 cut(s) 1100, 1547
Mph1103I ATGCAT 3 cut(s) 1592, 1669, 2218
MroI TCCGGA 3 cut(s) 10, 79, 194
MroXI GAANNNNTTC 1 cut(s) 1412
MscI TGGCCA 2 cut(s) 1100, 1547
MseI TTAA 6 cut(s) 642, 1179, 1475, 1967, 2283, 2366
Msp20I TGGCCA 2 cut(s) 1100, 1547
MspA1I CMGCKG 2 cut(s) 133, 529
MspCI CTTAAG 2 cut(s) 1474, 2365
MspR9I CCNGG 3 cut(s) 915, 1736, 2237
Mva1269I GAATGC 3 cut(s) 1667, 1958, 2429
MvaI CCWGG 3 cut(s) 915, 1736, 2237
MwoI GCNNNNNNNGC 6 cut(s) 197, 493, 609, 667, 1087, 2318
NdeI CATATG 2 cut(s) 1153, 1190
NlaIII CATG 6 cut(s) 1054, 1219, 1441, 1450, 1648, 1927
NlaIV GGNNCC 7 cut(s) 9, 15, 372, 495, 2096, 2165, 2241
NmeAIII GCCGAG 4 cut(s) 265, 465, 817, 1520
NmuCI GTSAC 2 cut(s) 424, 2075
NsiI ATGCAT 3 cut(s) 1592, 1669, 2218
PagI TCATGA 2 cut(s) 1050, 1446
PaqCI CACCTGC 1 cut(s) 552
PceI AGGCCT 2 cut(s) 718, 945
PciSI GCTCTTC 1 cut(s) 858
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 297, 311
PctI GAATGC 3 cut(s) 1667, 1958, 2429
PdmI GAANNNNTTC 1 cut(s) 1412
PfeI GAWTC 7 cut(s) 229, 446, 938, 1450, 1538, 1611, 1724
PfoI TCCNGGA 1 cut(s) 1734
PinAI ACCGGT 2 cut(s) 379, 471
Ple19I CGATCG 1 cut(s) 253
PleI GAGTC 5 cut(s) 167, 353, 608, 715, 2498
PmaCI CACGTG 1 cut(s) 1688
PmlI CACGTG 1 cut(s) 1688
PpsI GAGTC 5 cut(s) 167, 353, 608, 715, 2498
Ppu21I YACGTR 1 cut(s) 1688
PpuMI RGGWCCY 1 cut(s) 2449
Psp124BI GAGCTC 1 cut(s) 2355
Psp1406I AACGTT 1 cut(s) 1501
Psp5II RGGWCCY 1 cut(s) 2449
Psp6I CCWGG 3 cut(s) 913, 1734, 2235
PspCI CACGTG 1 cut(s) 1688
PspFI CCCAGC 1 cut(s) 868
PspGI CCWGG 3 cut(s) 913, 1734, 2235
PspN4I GGNNCC 7 cut(s) 9, 15, 372, 495, 2096, 2165, 2241
PspOMI GGGCCC 1 cut(s) 2094
PspPI GGNCC 9 cut(s) 125, 474, 519, 789, 1299, 1492, 2094, 2095, 2449
PspPPI RGGWCCY 1 cut(s) 2449
PsuI RGATCY 2 cut(s) 1273, 1361
PvuI CGATCG 1 cut(s) 253
SacI GAGCTC 1 cut(s) 2355
SalI GTCGAC 1 cut(s) 164
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 6 cut(s) 642, 1179, 1475, 1967, 2283, 2366
Sau96I GGNCC 9 cut(s) 125, 474, 519, 789, 1299, 1492, 2094, 2095, 2449
ScaI AGTACT 2 cut(s) 623, 1472
SchI GAGTC 5 cut(s) 167, 353, 608, 716, 2498
ScrFI CCNGG 3 cut(s) 915, 1736, 2237
SduI GDGCHC 4 cut(s) 1092, 1115, 2098, 2355
SfaNI GCATC 5 cut(s) 45, 1012, 1599, 1654, 2203
SfcI CTRYAG 3 cut(s) 625, 699, 1806
SinI GGWCC 3 cut(s) 474, 1299, 2449
SmlI CTYRAG 5 cut(s) 1091, 1474, 1487, 2365, 2453
SmoI CTYRAG 5 cut(s) 1091, 1474, 1487, 2365, 2453
Sse9I AATT 7 cut(s) 344, 1760, 1898, 1983, 2123, 2342, 2479
SseBI AGGCCT 2 cut(s) 718, 945
SsiI CCGC 9 cut(s) 5, 131, 294, 416, 419, 485, 527, 2288, 2370
SspMI CTAG 6 cut(s) 720, 750, 1371, 1895, 2309, 2403
SstI GAGCTC 1 cut(s) 2355
StuI AGGCCT 2 cut(s) 718, 945
StyD4I CCNGG 3 cut(s) 913, 1734, 2235
StyI CCWWGG 3 cut(s) 150, 1542, 2098
TaaI ACNGT 2 cut(s) 626, 635
TaiI ACGT 4 cut(s) 320, 1504, 1690, 2046
TasI AATT 7 cut(s) 344, 1760, 1898, 1983, 2123, 2342, 2479
TatI WGTACW 5 cut(s) 621, 1464, 1470, 2436, 2516
TauI GCSGC 6 cut(s) 8, 296, 419, 487, 529, 2290
TfiI GAWTC 7 cut(s) 229, 446, 938, 1450, 1538, 1611, 1724
Tru1I TTAA 6 cut(s) 642, 1179, 1475, 1967, 2283, 2366
Tru9I TTAA 6 cut(s) 642, 1179, 1475, 1967, 2283, 2366
TscAI CASTG 4 cut(s) 1115, 1555, 2058, 2343
TseFI GTSAC 2 cut(s) 424, 2075
TseI GCWGC 7 cut(s) 58, 133, 188, 558, 684, 694, 1679
Tsp45I GTSAC 2 cut(s) 424, 2075
TspGWI ACGGA 2 cut(s) 470, 1953
TspRI CASTG 4 cut(s) 1115, 1555, 2058, 2343
Vha464I CTTAAG 2 cut(s) 1474, 2365
VpaK11BI GGWCC 3 cut(s) 474, 1299, 2449
XapI RAATTY 3 cut(s) 344, 1898, 2342
XbaI TCTAGA 1 cut(s) 1894
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 1412
XspI CTAG 6 cut(s) 720, 750, 1371, 1895, 2309, 2403
ZraI GACGTC 1 cut(s) 318
ZrmI AGTACT 2 cut(s) 623, 1472
Zsp2I ATGCAT 3 cut(s) 1592, 1669, 2218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.