Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001374

Physical Location & Seq

Genomic Coordinates Reverse (-)

31519078 .. 31535119

Gene Structure 16042 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1250500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 681 bp

ATGGAAGCACAGGAGGTGGAGGCGCAGGCGGCGACTGGAAGTGGCCAACAACAACTCAAGCTTTATTCCTACTGGATGAGCTCTTGCTCCTTCCGTGTCCGAATTGCTCTCAACTTGAAAGGGCTGAAATACGAGTACAAAGCTCTGGCAAAGGGAGAGCAATTCAGTCCCGAGTTCAGAAAGCTGAATCCGATGGGGTATGTGCCTGTGCTTGTGGATGGGGACACCGTTGTTGCTGACTCATTTGCCATCATATTGTATTTAGAAGAAAAGTACCCGCAGCATCCATTGTTACCACCAGATCTTCAGAAAAAGGCTATCAATTACCAGGCTGCAAATATTGTTTCCTCAAGCATACAACCTCTACAGAATATGACTGTACTGAAGTACATTGAAGAAAAGGTTAGGCCCGTTGAGAAACTTGAATGGGTTCAATTTCATATTGGAAAAGGCTTTTTAGCACTTGAAGAACTGCTGAACAACCATGCAGGAAAATATGCAACTGGAGATGAAGTTTACATGGCAGATTTGTTTCTGGCACCACAGCTTTATGCAGCCATTACAAGGTTCCAGCTAGACATGACTCAGTTTCCCCTTTTGGCCAGGTTGCATGAGGCATACAATAAGATACCAGCATTCCTAGATGCTTTACCAGAAAAGCAGCCGGATGCTCCTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006725 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009636 GO:0009987 GO:0016034 GO:0016054 GO:0016853 GO:0016859 GO:0018958 GO:0019336 GO:0019439 GO:0019748 GO:0019752 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044248 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0050896 GO:0071704 GO:0072329 GO:0098754 GO:1901360 GO:1901361 GO:1901575 GO:1901615 GO:1901616 GO:1901999 GO:1902000

KEGG Pathways

Metabolic & Signaling

ko00350 ko00643 ko01100 ko01120 map00350 map00643 map01100 map01120

Pfam Domains

Protein Families

GST_C GST_C_2 GST_C_3 GST_N GST_N_3

Protein Analysis

227

Amino Acids

25.6

Weight (kDa)

5.63

Isoelectric Point (pI)

45.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	18 - 89	1.2e-16	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	21 - 95	9.7e-19	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	27 - 90	6.5e-18	Glutathione S-transferase, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001374)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02380 AT2G02390 AT2G02390 AT2G02390 AT2G02390
fragaria_vesca	FvH4_5g27160
malus_domestica	MD08G1250500.v1.1 MD15G1445700.v1.1
prunus_persica	Prupe.1G587700_v2.0.a1 Prupe.1G587700_v2.0.a1
pyrus_communis	pycom08g21730 pycom15g39250
rosa_chinensis	RchiOBHm_Chr7g0218201 RchiOBHm_Chr7g0218231 RchiOBHm_Chr7g0218251
rosa_laevigata	RLG00000002386 RLG00000002388 RLG00000002390
rosa_multiflora	Rmu_sc0004956.1_g000003 Rmu_sc0008593.1_g000004 Rmu_sc0008593.1_g000008 Rmu_sc0011666.1_g000005 Rmu_sc0021950.1_g000001 Rmu_sc0024798.1_g000001
rosa_roxburghii	Rroxscaffold_1G00032070 Rroxscaffold_3G00241200 Rroxscaffold_3G00241210
rosa_rugosa	Rorug07G0174800 Rorug07G0174900 Rorug07G0175000 Rorug07G0175200 Rorug07G0175300 Rorug07G0175400 Rorug07G0175500 Rorug07G0175500 Rorug07G0175600 Rorug07G0175700 Rorug07G0175800
rosa_samantha	Rh7AG304100 Rh7AG304200 Rh7BG308500 Rh7BG308600 Rh7BG308700 Rh7BG308800 Rh7CG322500 Rh7CG322700 Rh7CG334300 Rh7DG304300 Rh7DG316400
rosa_wichuraiana	Rw7G025830 Rw7G026860 Rw7G026940 Rw7G026950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	538
AciI	CCGC	2 cut(s)	29, 278
AcoI	YGGCCR	2 cut(s)	43, 600
AcuI	CTGAAG	2 cut(s)	290, 404
AfaI	GTAC	4 cut(s)	137, 275, 381, 389
AfiI	CCNNNNNNNGG	1 cut(s)	564
AgsI	TTSAA	5 cut(s)	118, 395, 425, 434, 467
AjnI	CCWGG	2 cut(s)	327, 602
AluBI	AGCT	6 cut(s)	61, 81, 143, 184, 547, 574
AluI	AGCT	6 cut(s)	61, 81, 143, 184, 547, 574
Alw21I	GWGCWC	1 cut(s)	83
Ama87I	CYCGRG	1 cut(s)	170
AoxI	GGCC	3 cut(s)	43, 407, 600
ApeKI	GCWGC	4 cut(s)	280, 332, 554, 661
Asp700I	GAANNNNTTC	1 cut(s)	429
AspLEI	GCGC	1 cut(s)	25
AspS9I	GGNCC	1 cut(s)	408
AvaI	CYCGRG	1 cut(s)	170
BalI	TGGCCA	2 cut(s)	45, 602
BanI	GGYRCC	1 cut(s)	538
BanII	GRGCYC	1 cut(s)	83
Bbv12I	GWGCWC	1 cut(s)	83
BbvI	GCAGC	4 cut(s)	292, 319, 566, 673
BccI	CCATC	3 cut(s)	187, 212, 257
BciT130I	CCWGG	2 cut(s)	329, 604
BfaI	CTAG	2 cut(s)	575, 641
BfmI	CTRYAG	1 cut(s)	365
BglII	AGATCT	1 cut(s)	301
BisI	GCNGC	5 cut(s)	30, 281, 333, 555, 662
BlsI	GCNGC	5 cut(s)	31, 282, 334, 556, 663
Bme1390I	CCNGG	2 cut(s)	329, 604
BmeT110I	CYCGRG	1 cut(s)	170
BmgT120I	GGNCC	1 cut(s)	408
BmiI	GGNNCC	2 cut(s)	540, 569
BmrFI	CCNGG	2 cut(s)	329, 604
BmsI	GCATC	3 cut(s)	292, 634, 658
BpmI	CTGGAG	1 cut(s)	525
BpuEI	CTTGAG	2 cut(s)	41, 334
BsaXI	ACNNNNNCTCC	2 cut(s)	498, 528
Bsc4I	CCNNNNNNNGG	1 cut(s)	564
Bse1I	ACTGG	3 cut(s)	40, 77, 508
BseBI	CCWGG	2 cut(s)	329, 604
BseGI	GGATG	4 cut(s)	81, 223, 283, 673
BseLI	CCNNNNNNNGG	1 cut(s)	564
BseMII	CTCAG	1 cut(s)	599
BseNI	ACTGG	3 cut(s)	40, 77, 508
BseXI	GCAGC	4 cut(s)	292, 319, 566, 673
BshFI	GGCC	3 cut(s)	45, 409, 602
BshNI	GGYRCC	1 cut(s)	538
BsiHKAI	GWGCWC	1 cut(s)	83
BsiHKCI	CYCGRG	1 cut(s)	170
BsiSI	CCGG	1 cut(s)	665
BslFI	GGGAC	2 cut(s)	153, 236
BslI	CCNNNNNNNGG	1 cut(s)	564
BsmFI	GGGAC	2 cut(s)	153, 236
BsmI	GAATGC	1 cut(s)	635
BsnI	GGCC	3 cut(s)	45, 409, 602
BsoBI	CYCGRG	1 cut(s)	170
Bsp1286I	GDGCHC	1 cut(s)	83
Bsp143I	GATC	1 cut(s)	301
BspACI	CCGC	2 cut(s)	29, 278
BspANI	GGCC	3 cut(s)	45, 409, 602
BspCNI	CTCAG	1 cut(s)	598
BspLI	GGNNCC	2 cut(s)	540, 569
BspT107I	GGYRCC	1 cut(s)	538
BsrI	ACTGG	3 cut(s)	40, 77, 508
BssMI	GATC	1 cut(s)	301
Bst2UI	CCWGG	2 cut(s)	329, 604
Bst4CI	ACNGT	2 cut(s)	229, 379
BstC8I	GCNNGC	1 cut(s)	27
BstDEI	CTNAG	1 cut(s)	585
BstF5I	GGATG	4 cut(s)	81, 223, 283, 673
BstHHI	GCGC	1 cut(s)	25
BstKTI	GATC	1 cut(s)	304
BstMBI	GATC	1 cut(s)	301
BstMWI	GCNNNNNNNGC	1 cut(s)	29
BstNI	CCWGG	2 cut(s)	329, 604
BstSCI	CCNGG	2 cut(s)	327, 602
BstSFI	CTRYAG	1 cut(s)	365
BstV1I	GCAGC	4 cut(s)	292, 319, 566, 673
BstX2I	RGATCY	1 cut(s)	301
BstYI	RGATCY	1 cut(s)	301
BsuRI	GGCC	3 cut(s)	45, 409, 602
BtsCI	GGATG	4 cut(s)	81, 223, 283, 673
Cac8I	GCNNGC	1 cut(s)	27
CfoI	GCGC	1 cut(s)	25
Cfr13I	GGNCC	1 cut(s)	408
Csp6I	GTAC	4 cut(s)	136, 274, 380, 388
CviAII	CATG	4 cut(s)	485, 520, 580, 611
CviQI	GTAC	4 cut(s)	136, 274, 380, 388
DdeI	CTNAG	1 cut(s)	585
DpnI	GATC	1 cut(s)	303
DpnII	GATC	1 cut(s)	301
EaeI	YGGCCR	2 cut(s)	43, 600
Ecl136II	GAGCTC	1 cut(s)	81
Eco24I	GRGCYC	1 cut(s)	83
Eco53kI	GAGCTC	1 cut(s)	81
Eco57I	CTGAAG	2 cut(s)	290, 404
Eco88I	CYCGRG	1 cut(s)	170
EcoICRI	GAGCTC	1 cut(s)	81
EcoRII	CCWGG	2 cut(s)	327, 602
EcoT38I	GRGCYC	1 cut(s)	83
FaeI	CATG	4 cut(s)	488, 523, 583, 614
FaqI	GGGAC	2 cut(s)	153, 236
FatI	CATG	4 cut(s)	484, 519, 579, 610
FauI	CCCGC	1 cut(s)	285
Fnu4HI	GCNGC	5 cut(s)	30, 281, 333, 555, 662
FokI	GGATG	3 cut(s)	88, 230, 270
FriOI	GRGCYC	1 cut(s)	83
Fsp4HI	GCNGC	5 cut(s)	30, 281, 333, 555, 662
FspBI	CTAG	2 cut(s)	575, 641
GlaI	GCGC	1 cut(s)	24
GluI	GCNGC	5 cut(s)	30, 281, 333, 555, 662
GsuI	CTGGAG	1 cut(s)	525
HaeIII	GGCC	3 cut(s)	45, 409, 602
HapII	CCGG	1 cut(s)	665
HhaI	GCGC	1 cut(s)	25
Hin1II	CATG	4 cut(s)	488, 523, 583, 614
Hin6I	GCGC	1 cut(s)	23
HinP1I	GCGC	1 cut(s)	23
HindIII	AAGCTT	1 cut(s)	59
HinfI	GANTC	3 cut(s)	187, 239, 583
HpaII	CCGG	1 cut(s)	665
Hpy166II	GTNNAC	1 cut(s)	517
Hpy188I	TCNGA	4 cut(s)	101, 179, 192, 309
Hpy188III	TCNNGA	1 cut(s)	170
Hpy8I	GTNNAC	1 cut(s)	517
HpyAV	CCTTC	1 cut(s)	100
HpyCH4III	ACNGT	2 cut(s)	229, 379
HpyCH4V	TGCA	5 cut(s)	335, 488, 500, 554, 610
HpyF10VI	GCNNNNNNNGC	1 cut(s)	29
HpyF3I	CTNAG	1 cut(s)	585
Hsp92II	CATG	4 cut(s)	488, 523, 583, 614
HspAI	GCGC	1 cut(s)	23
Kzo9I	GATC	1 cut(s)	301
LmnI	GCTCC	2 cut(s)	92, 676
Lsp1109I	GCAGC	4 cut(s)	292, 319, 566, 673
LweI	GCATC	3 cut(s)	292, 634, 658
MaeI	CTAG	2 cut(s)	575, 641
MaeIII	GTNAC	1 cut(s)	291
MalI	GATC	1 cut(s)	303
MboI	GATC	1 cut(s)	301
MboII	GAAGA	4 cut(s)	278, 296, 407, 479
MflI	RGATCY	1 cut(s)	301
MhlI	GDGCHC	1 cut(s)	83
MlsI	TGGCCA	2 cut(s)	45, 602
MluCI	AATT	4 cut(s)	102, 161, 322, 434
MluNI	TGGCCA	2 cut(s)	45, 602
MlyI	GAGTC	2 cut(s)	233, 577
MnlI	CCTC	5 cut(s)	7, 13, 358, 372, 607
Mox20I	TGGCCA	2 cut(s)	45, 602
MroXI	GAANNNNTTC	1 cut(s)	429
MscI	TGGCCA	2 cut(s)	45, 602
Msp20I	TGGCCA	2 cut(s)	45, 602
MspI	CCGG	1 cut(s)	665
MspR9I	CCNGG	2 cut(s)	329, 604
Mva1269I	GAATGC	1 cut(s)	635
MvaI	CCWGG	2 cut(s)	329, 604
MwoI	GCNNNNNNNGC	1 cut(s)	29
NdeII	GATC	1 cut(s)	301
NlaIII	CATG	4 cut(s)	488, 523, 583, 614
NlaIV	GGNNCC	2 cut(s)	540, 569
PctI	GAATGC	1 cut(s)	635
PdmI	GAANNNNTTC	1 cut(s)	429
PfeI	GAWTC	1 cut(s)	187
PkrI	GCNGC	5 cut(s)	31, 282, 334, 556, 663
PleI	GAGTC	2 cut(s)	233, 577
PpsI	GAGTC	2 cut(s)	233, 577
Psp124BI	GAGCTC	1 cut(s)	83
Psp6I	CCWGG	2 cut(s)	327, 602
PspGI	CCWGG	2 cut(s)	327, 602
PspN4I	GGNNCC	2 cut(s)	540, 569
PspPI	GGNCC	1 cut(s)	408
PsuI	RGATCY	1 cut(s)	301
RsaI	GTAC	4 cut(s)	137, 275, 381, 389
RsaNI	GTAC	4 cut(s)	136, 274, 380, 388
SacI	GAGCTC	1 cut(s)	83
SatI	GCNGC	5 cut(s)	30, 281, 333, 555, 662
Sau3AI	GATC	1 cut(s)	301
Sau96I	GGNCC	1 cut(s)	408
SchI	GAGTC	2 cut(s)	233, 577
ScrFI	CCNGG	2 cut(s)	329, 604
SduI	GDGCHC	1 cut(s)	83
SfaNI	GCATC	3 cut(s)	292, 634, 658
SfcI	CTRYAG	1 cut(s)	365
SmlI	CTYRAG	2 cut(s)	56, 349
SmoI	CTYRAG	2 cut(s)	56, 349
Sse9I	AATT	4 cut(s)	102, 161, 322, 434
SsiI	CCGC	2 cut(s)	29, 278
SspI	AATATT	1 cut(s)	340
SspMI	CTAG	2 cut(s)	575, 641
SstI	GAGCTC	1 cut(s)	83
StyD4I	CCNGG	2 cut(s)	327, 602
TaaI	ACNGT	2 cut(s)	229, 379
TasI	AATT	4 cut(s)	102, 161, 322, 434
TatI	WGTACW	3 cut(s)	135, 379, 387
TauI	GCSGC	1 cut(s)	32
TfiI	GAWTC	1 cut(s)	187
TseI	GCWGC	4 cut(s)	280, 332, 554, 661
TspDTI	ATGAA	3 cut(s)	428, 525, 666
TspGWI	ACGGA	1 cut(s)	83
XmnI	GAANNNNTTC	1 cut(s)	429
XspI	CTAG	2 cut(s)	575, 641

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.