Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001374

Physical Location & Seq

Genomic Coordinates Reverse (-)

54879768 .. 54883933

Gene Structure 4166 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1445700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 702 bp

ATGATGGAAGCACAGGAGGTGGAGGTGGAGGTGGAGGCGCAGGCGGCGACTGAAAGTGGCGATCAACAAATCAAGCTTTATTCCTACTGGAGAAGCTCTTGCTCCTACCGTGTCCGAATTGCTCTCAACTTGAAAGGGTTGAAATACGAGTACAAAGCTGTAAACCTGCTGAAGGGAGAGCAATTCAGTCCCGAGTTCAGAAAGCTGAATCCGGTCGGGTATGTGCCTGTGCTTGTGGATGGGGACACCCTTGTTGCTGACTCATTTGCCATCCTAATGCATTTAGAAGAAAAGTACCCGCAACATCCATTGTTACCGCCCGATCTTCAGAAAAGGGCTATCAATTACCAGGCTGCAAATATTGTTTCCTCAAGCATACAACCTCTACAGAATCTGGCTGTACTGAAGTACCTTGAAGAAAAAGTTAGTCCCGATGAGAAAATTGAATGGGTTAAATTTCATATTGGAAAAGGCTTTTCAGCACTTGAAGAACTGCTGAACAACCATGCAGGAAAATATGCCACTGGAGATGAAGTTTACATGGCAGATTTGTTTCTGGCACCCCAGGTTTATACAGCCATTAGGTTCCAGCTAGACATGACTCAGTTCCCCCTTTTGGCCAGGTTGCATGAGGCATACAAAAAGATACCGGCATTCCTAGATGCTATACCAGAAAAGCAGCCGGATGCTCCTTCTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006725 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009636 GO:0009987 GO:0016034 GO:0016054 GO:0016853 GO:0016859 GO:0018958 GO:0019336 GO:0019439 GO:0019748 GO:0019752 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044248 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0050896 GO:0071704 GO:0072329 GO:0098754 GO:1901360 GO:1901361 GO:1901575 GO:1901615 GO:1901616 GO:1901999 GO:1902000

KEGG Pathways

Metabolic & Signaling

ko00350 ko00643 ko01100 ko01120 map00350 map00643 map01100 map01120

Pfam Domains

Protein Families

GST_C GST_C_2 GST_C_3 GST_N GST_N_3

Protein Analysis

234

Amino Acids

26.45

Weight (kDa)

5.32

Isoelectric Point (pI)

45.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	23 - 96	1.5e-16	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	26 - 102	5.9e-17	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	32 - 97	1.9e-15	Glutathione S-transferase, N-terminal domain
GST_C_3	PF14497	137 - 217	2.5e-06	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001374)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02380 AT2G02390 AT2G02390 AT2G02390 AT2G02390
fragaria_vesca	FvH4_5g27160
malus_domestica	MD08G1250500.v1.1 MD15G1445700.v1.1
prunus_persica	Prupe.1G587700_v2.0.a1 Prupe.1G587700_v2.0.a1
pyrus_communis	pycom08g21730 pycom15g39250
rosa_chinensis	RchiOBHm_Chr7g0218201 RchiOBHm_Chr7g0218231 RchiOBHm_Chr7g0218251
rosa_laevigata	RLG00000002386 RLG00000002388 RLG00000002390
rosa_multiflora	Rmu_sc0004956.1_g000003 Rmu_sc0008593.1_g000004 Rmu_sc0008593.1_g000008 Rmu_sc0011666.1_g000005 Rmu_sc0021950.1_g000001 Rmu_sc0024798.1_g000001
rosa_roxburghii	Rroxscaffold_1G00032070 Rroxscaffold_3G00241200 Rroxscaffold_3G00241210
rosa_rugosa	Rorug07G0174800 Rorug07G0174900 Rorug07G0175000 Rorug07G0175200 Rorug07G0175300 Rorug07G0175400 Rorug07G0175500 Rorug07G0175500 Rorug07G0175600 Rorug07G0175700 Rorug07G0175800
rosa_samantha	Rh7AG304100 Rh7AG304200 Rh7BG308500 Rh7BG308600 Rh7BG308700 Rh7BG308800 Rh7CG322500 Rh7CG322700 Rh7CG334300 Rh7DG304300 Rh7DG316400
rosa_wichuraiana	Rw7G025830 Rw7G026860 Rw7G026940 Rw7G026950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	174
AccB1I	GGYRCC	1 cut(s)	559
AciI	CCGC	3 cut(s)	44, 299, 317
AcoI	YGGCCR	1 cut(s)	618
AcsI	RAATTY	1 cut(s)	455
AcuI	CTGAAG	3 cut(s)	191, 311, 425
AfaI	GTAC	4 cut(s)	152, 296, 402, 410
AfiI	CCNNNNNNNGG	2 cut(s)	172, 616
AgsI	TTSAA	5 cut(s)	133, 142, 416, 446, 488
AjnI	CCWGG	3 cut(s)	348, 564, 620
AluBI	AGCT	5 cut(s)	76, 96, 158, 205, 592
AluI	AGCT	5 cut(s)	76, 96, 158, 205, 592
AlwNI	CAGNNNCTG	1 cut(s)	394
Ama87I	CYCGRG	1 cut(s)	191
AoxI	GGCC	1 cut(s)	618
ApeKI	GCWGC	2 cut(s)	353, 679
ApoI	RAATTY	1 cut(s)	455
AspLEI	GCGC	1 cut(s)	40
AvaI	CYCGRG	1 cut(s)	191
BaeI	ACNNNNGTAYC	2 cut(s)	392, 425
BalI	TGGCCA	1 cut(s)	620
BanI	GGYRCC	1 cut(s)	559
BbvI	GCAGC	2 cut(s)	340, 691
BccI	CCATC	2 cut(s)	233, 278
BciT130I	CCWGG	3 cut(s)	350, 566, 622
BfaI	CTAG	2 cut(s)	593, 659
BfmI	CTRYAG	1 cut(s)	386
BfuAI	ACCTGC	1 cut(s)	174
BisI	GCNGC	3 cut(s)	45, 354, 680
BlsI	GCNGC	3 cut(s)	46, 355, 681
Bme1390I	CCNGG	3 cut(s)	350, 566, 622
BmeT110I	CYCGRG	1 cut(s)	191
BmiI	GGNNCC	2 cut(s)	561, 587
BmrFI	CCNGG	3 cut(s)	350, 566, 622
BmsI	GCATC	2 cut(s)	652, 676
BpmI	CTGGAG	2 cut(s)	109, 546
BpuEI	CTTGAG	1 cut(s)	355
BsaJI	CCNNGG	1 cut(s)	564
BsaWI	WCCGGW	1 cut(s)	211
BsaXI	ACNNNNNCTCC	2 cut(s)	519, 549
Bsc4I	CCNNNNNNNGG	2 cut(s)	172, 616
Bse118I	RCCGGY	1 cut(s)	649
Bse1I	ACTGG	2 cut(s)	92, 529
BseBI	CCWGG	3 cut(s)	350, 566, 622
BseDI	CCNNGG	1 cut(s)	564
BseGI	GGATG	4 cut(s)	244, 270, 304, 691
BseLI	CCNNNNNNNGG	2 cut(s)	172, 616
BseMII	CTCAG	1 cut(s)	617
BseNI	ACTGG	2 cut(s)	92, 529
BseXI	GCAGC	2 cut(s)	340, 691
Bsh1285I	CGRYCG	1 cut(s)	216
BshFI	GGCC	1 cut(s)	620
BshNI	GGYRCC	1 cut(s)	559
BsiEI	CGRYCG	1 cut(s)	216
BsiHKCI	CYCGRG	1 cut(s)	191
BsiSI	CCGG	3 cut(s)	212, 650, 683
BslFI	GGGAC	3 cut(s)	174, 257, 414
BslI	CCNNNNNNNGG	2 cut(s)	172, 616
BsmFI	GGGAC	3 cut(s)	174, 257, 414
BsmI	GAATGC	1 cut(s)	653
BsnI	GGCC	1 cut(s)	620
BsoBI	CYCGRG	1 cut(s)	191
Bsp143I	GATC	2 cut(s)	61, 322
BspACI	CCGC	3 cut(s)	44, 299, 317
BspANI	GGCC	1 cut(s)	620
BspCNI	CTCAG	1 cut(s)	616
BspLI	GGNNCC	2 cut(s)	561, 587
BspMI	ACCTGC	1 cut(s)	174
BspT107I	GGYRCC	1 cut(s)	559
BsrFI	RCCGGY	1 cut(s)	649
BsrI	ACTGG	2 cut(s)	92, 529
BssAI	RCCGGY	1 cut(s)	649
BssECI	CCNNGG	1 cut(s)	564
BssMI	GATC	2 cut(s)	61, 322
Bst2UI	CCWGG	3 cut(s)	350, 566, 622
Bst4CI	ACNGT	1 cut(s)	110
BstC8I	GCNNGC	1 cut(s)	42
BstDEI	CTNAG	1 cut(s)	603
BstENI	CCTNNNNNAGG	1 cut(s)	170
BstF5I	GGATG	4 cut(s)	244, 270, 304, 691
BstHHI	GCGC	1 cut(s)	40
BstKTI	GATC	2 cut(s)	64, 325
BstMBI	GATC	2 cut(s)	61, 322
BstMCI	CGRYCG	1 cut(s)	216
BstMWI	GCNNNNNNNGC	1 cut(s)	44
BstNI	CCWGG	3 cut(s)	350, 566, 622
BstSCI	CCNGG	3 cut(s)	348, 564, 620
BstSFI	CTRYAG	1 cut(s)	386
BstV1I	GCAGC	2 cut(s)	340, 691
BsuRI	GGCC	1 cut(s)	620
BtsCI	GGATG	4 cut(s)	244, 270, 304, 691
BtsIMutI	CAGTG	1 cut(s)	522
BveI	ACCTGC	1 cut(s)	174
Cac8I	GCNNGC	1 cut(s)	42
CaiI	CAGNNNCTG	1 cut(s)	394
CfoI	GCGC	1 cut(s)	40
Cfr10I	RCCGGY	1 cut(s)	649
Csp6I	GTAC	4 cut(s)	151, 295, 401, 409
CviAII	CATG	4 cut(s)	506, 541, 598, 629
CviQI	GTAC	4 cut(s)	151, 295, 401, 409
DdeI	CTNAG	1 cut(s)	603
DpnI	GATC	2 cut(s)	63, 324
DpnII	GATC	2 cut(s)	61, 322
EaeI	YGGCCR	1 cut(s)	618
Eco57I	CTGAAG	3 cut(s)	191, 311, 425
Eco88I	CYCGRG	1 cut(s)	191
EcoNI	CCTNNNNNAGG	1 cut(s)	170
EcoRII	CCWGG	3 cut(s)	348, 564, 620
EcoT22I	ATGCAT	1 cut(s)	282
FaeI	CATG	4 cut(s)	509, 544, 601, 632
FaqI	GGGAC	3 cut(s)	174, 257, 414
FatI	CATG	4 cut(s)	505, 540, 597, 628
FauI	CCCGC	1 cut(s)	306
Fnu4HI	GCNGC	3 cut(s)	45, 354, 680
FokI	GGATG	4 cut(s)	251, 257, 291, 698
Fsp4HI	GCNGC	3 cut(s)	45, 354, 680
FspBI	CTAG	2 cut(s)	593, 659
GlaI	GCGC	1 cut(s)	39
GluI	GCNGC	3 cut(s)	45, 354, 680
GsuI	CTGGAG	2 cut(s)	109, 546
HaeIII	GGCC	1 cut(s)	620
HapII	CCGG	3 cut(s)	212, 650, 683
HhaI	GCGC	1 cut(s)	40
Hin1II	CATG	4 cut(s)	509, 544, 601, 632
Hin6I	GCGC	1 cut(s)	38
HinP1I	GCGC	1 cut(s)	38
HindIII	AAGCTT	1 cut(s)	74
HinfI	GANTC	4 cut(s)	208, 260, 391, 601
HpaII	CCGG	3 cut(s)	212, 650, 683
Hpy166II	GTNNAC	2 cut(s)	163, 538
Hpy188I	TCNGA	3 cut(s)	116, 200, 330
Hpy188III	TCNNGA	2 cut(s)	191, 431
Hpy8I	GTNNAC	2 cut(s)	163, 538
HpyAV	CCTTC	2 cut(s)	166, 702
HpyCH4III	ACNGT	1 cut(s)	110
HpyCH4V	TGCA	4 cut(s)	280, 356, 509, 628
HpyF10VI	GCNNNNNNNGC	1 cut(s)	44
HpyF3I	CTNAG	1 cut(s)	603
Hsp92II	CATG	4 cut(s)	509, 544, 601, 632
HspAI	GCGC	1 cut(s)	38
Kzo9I	GATC	2 cut(s)	61, 322
LmnI	GCTCC	2 cut(s)	107, 694
Lsp1109I	GCAGC	2 cut(s)	340, 691
LweI	GCATC	2 cut(s)	652, 676
MaeI	CTAG	2 cut(s)	593, 659
MaeIII	GTNAC	1 cut(s)	312
MalI	GATC	2 cut(s)	63, 324
MboI	GATC	2 cut(s)	61, 322
MboII	GAAGA	4 cut(s)	299, 317, 428, 500
MlsI	TGGCCA	1 cut(s)	620
MluCI	AATT	5 cut(s)	117, 182, 343, 441, 455
MluNI	TGGCCA	1 cut(s)	620
MlyI	GAGTC	2 cut(s)	254, 595
MnlI	CCTC	7 cut(s)	10, 16, 22, 28, 379, 393, 625
Mox20I	TGGCCA	1 cut(s)	620
Mph1103I	ATGCAT	1 cut(s)	282
MscI	TGGCCA	1 cut(s)	620
MseI	TTAA	1 cut(s)	453
MslI	CAYNNNNRTG	1 cut(s)	275
Msp20I	TGGCCA	1 cut(s)	620
MspI	CCGG	3 cut(s)	212, 650, 683
MspR9I	CCNGG	3 cut(s)	350, 566, 622
Mva1269I	GAATGC	1 cut(s)	653
MvaI	CCWGG	3 cut(s)	350, 566, 622
MwoI	GCNNNNNNNGC	1 cut(s)	44
NdeII	GATC	2 cut(s)	61, 322
NlaIII	CATG	4 cut(s)	509, 544, 601, 632
NlaIV	GGNNCC	2 cut(s)	561, 587
NsiI	ATGCAT	1 cut(s)	282
PctI	GAATGC	1 cut(s)	653
PfeI	GAWTC	2 cut(s)	208, 391
PkrI	GCNGC	3 cut(s)	46, 355, 681
PleI	GAGTC	2 cut(s)	254, 595
PpsI	GAGTC	2 cut(s)	254, 595
Psp6I	CCWGG	3 cut(s)	348, 564, 620
PspGI	CCWGG	3 cut(s)	348, 564, 620
PspN4I	GGNNCC	2 cut(s)	561, 587
PstNI	CAGNNNCTG	1 cut(s)	394
RsaI	GTAC	4 cut(s)	152, 296, 402, 410
RsaNI	GTAC	4 cut(s)	151, 295, 401, 409
RseI	CAYNNNNRTG	1 cut(s)	275
SaqAI	TTAA	1 cut(s)	453
SatI	GCNGC	3 cut(s)	45, 354, 680
Sau3AI	GATC	2 cut(s)	61, 322
SchI	GAGTC	2 cut(s)	254, 595
ScrFI	CCNGG	3 cut(s)	350, 566, 622
SfaNI	GCATC	2 cut(s)	652, 676
SfcI	CTRYAG	1 cut(s)	386
SmiMI	CAYNNNNRTG	1 cut(s)	275
SmlI	CTYRAG	1 cut(s)	370
SmoI	CTYRAG	1 cut(s)	370
Sse9I	AATT	5 cut(s)	117, 182, 343, 441, 455
SsiI	CCGC	3 cut(s)	44, 299, 317
SspI	AATATT	1 cut(s)	361
SspMI	CTAG	2 cut(s)	593, 659
StyD4I	CCNGG	3 cut(s)	348, 564, 620
TaaI	ACNGT	1 cut(s)	110
TasI	AATT	5 cut(s)	117, 182, 343, 441, 455
TatI	WGTACW	2 cut(s)	150, 400
TauI	GCSGC	1 cut(s)	47
TfiI	GAWTC	2 cut(s)	208, 391
Tru1I	TTAA	1 cut(s)	453
Tru9I	TTAA	1 cut(s)	453
TscAI	CASTG	1 cut(s)	529
TseI	GCWGC	2 cut(s)	353, 679
TspDTI	ATGAA	2 cut(s)	449, 546
TspRI	CASTG	1 cut(s)	529
XagI	CCTNNNNNAGG	1 cut(s)	170
XapI	RAATTY	1 cut(s)	455
XspI	CTAG	2 cut(s)	593, 659
Zsp2I	ATGCAT	1 cut(s)	282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.